Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010745

Physical Location & Seq

Genomic Coordinates Forward (+)

17103632 .. 17108050

Gene Structure 4419 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1213000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3360 bp

ATGTTGTCAAATTTGGAAGGTGGGAAGAAGAGGGGCGGAGATTCCGGTAATCGGAAATTGTTGAAAGAGATTGAGAGTATAAGCAAAGCCCTATATGTAGACAAAAAACCGCCTAAGAGTTCAATTCCGGCAGGGAGTAACCCGTCAATGTCTCTAGGGAGGAACCGTTTACCTGACCCGAAATCGAAAAATAAGTATGGTGGTGAAAACTTGTTAAACAAAGAGAAGAGGTCCTTTTGGAATTGGAAGCCCTTGAAGGCGATTACTCATATCCGAAACCGTAGGTTTAACTGCTGCTTTTCTCTTCAAGTCCATTCCATTGAAGGGTTGCCCTCCACTTTGAATGAGATTAGTCTATGTGTACATTGGAAGAGGCGGGATGGTATTTTCGTTACTCATCCGGCAAAGATTGTTCAAGGAACGGCAAAATTTGAAGAGAAGTTGACTCACACATGCTCAGTATATGGTAGTAGGAGTGGGCCCCACCATTCGGCAAAGTATGAGGCGAAGCATTTTTTGCTATATGCTTCTGTGTTTGGTGCACCAGAACTAGATTTGGGGAAGCATAGGATTGACCTCACGAGGCTGCTTCCCCTGACATTGGAGGAGTTAGAGGAGGAGAAAAGCTCAGGGAACTGGACGACGAGTTTCAAATTATCAGGAAAGGCTAAAGGTGGTTCATTGAATGTTAGTTTTGGGTATACAGTTCTTGAAGATAATCCCAGTGCGACAGGAAATAGCCAGAATGTTTCTGAAGCATTGACTTCGAGACACAATAACTCAAGTATAAGGCGTGCTGAAACTCTTCCTAATCAGCAGTCACAGGCCTTGTCTCAATCTGTAGAGGGCATAAAGGATCTTCATGAGGTTTTGCCAGTATCAAGGTCGGAACTTTCTAGTTCGGTAAACACACTTTATCAGAAATTTGATGAAGAAGAGAAGTCAGATACTCCAGTTGACAAGCATCTTGATCCCATAAAAAGGAGTTCTTTCCCATCACCTGATTCTGGTAAAGAAGTAGAAAATGAATGTGAAGACAATGAATTTTCCATAGTTGAACAGGGCATAGAATTACCATCAAAGGAACTGGCGGAATCAGAAGTTGTTACACAGGCTGCTGATGCTTCTCCAGCTGAAAGCCATTTTTCTGAAATCACCACTGGCGTACAGGTAGCAGTTGAAGATGAAGTTGAGCTTGAATCCCAGGCTGAGGAAAAGGGTCGTACCAATGACCTTGTGGTAAGTGAGTCCACTTCCAACAGAGATGCCTTATGCACCAAAGAATCACTCATGAAAGAGCTAGAGTCTGCTTTAGGTGTTGTATCCAATTTGGAGAGAGCAGCACTAGAATCTTCTCCTGAAGACCAAAGATGTTATGTGGAAGGGAAATTAGACTCGAAAAAAAATATGATGGGGAGGTCCCATAGCTTGGATGATGTTACAGAATCTGTTGCTAATGAGTTCTTGAGCATGCTAGGGATAGAGCATAGTCCATTTTCATTGAGTTCTGAGAGTGATCCCGAGTCTCCAAGAGAGCGTCTGCTAAGACAATTTGAGAAGGAAGCCCTTGCTGGAGGTTGTTCTTTGTTTGATTTTGATGCAGGCATCAGTGACCAAACAGACTATGGCTACACACCTTCAACTGAGTCTGGTTGGGAGAATTTATCTGATAGTTTTGATTTCTCGTCTGTGATTCAAGCTGCAGAGGAAGAGCATCAGATAGCAGCTCAGGCAGTAAAAAGTAAAGCAAAGGCGAAAATGTTGGAGGACTTGGAGACAGAAGCATTAATGCGGGAGTGGGGCTTAAATGAGATGGCCTTTCAACATTCTCCACCAAAAAGTTGTGCTAGTTTTGGAAGTTCAATGGATTTGCCTGCTGAAGAACCTTTTGAATTGCCTCCTCTTGGAGATGGGTTAGGTCCTTTTCTTCAGACAAAGAATGGAGGGTTTGTGCGGTCCATGAATCCCTCACTTTTCAGCGAGGCCAAAAGTGGCGGGAACTTGATCATGCAGGTTTCTAGTCCAGTTGTGGTACCGGCAGAAATGGGTTCTGGGGTAATGGAGATATTGCAGCATTTGGCATCAGTAGGAATTGAAAAGCTTTCTATGCAAGCAAATAAGTTAATGCCCTTGGAAGATATCACTGGAAAGACAATGGAGCAAGTAGCATGGGAAGCTGCACCTACTTTGGAAGGACCTCAGAGGGAATTCGTGGTGCAGCATGAATCGGTTGGGCAGCATACATCCGATGGGCTAACAAGAGCTAAAGGAATTTCATCTGGGCCTAAGTCTAATAAGTTAAGTTCGAGCGCAGCTGGCAATGAGATGGGCTTAGAGTATGTCTCTTTAGAAGACCTTGCTCCTTTAGCCATGGATAAAATTGAAGCACTCTCAATTGAAGGGTTGAGGATACAAGCCGGGATGTCCGATGCAGATGCACCTTCAAACATCAGCGCACAGTCTGCTGCGGACATGTCAGCTCTCCAGGGCAAGGGTGTCAATGTTGGTGAATCCCTTGGTTTGGAAGGAGCTGCTGGGATGCAGTTGTTAGACATAAAAGACACTGGCAATGATGTTGACGGATTAATGGGCCTCTCATTAACTCTTGACGAATGGTTGAAACTTGATTCCGGTGAAATTGATGATGGTGATCATATTAGCGAACGGACTTCTCAAATCCTTGCGGCCCATCATGCAAACTCTTTGGACATGATTCGTGGAGGGTCAAGGGGAGAGAGAAGGCGTGGCAAAGGAGCTAGAAAGTGTGGCTTATTAGGGAACAATTTCACAGTAGCACTGATGGTGCAACTTCGTGATCCACTACGAAACTATGAACCTGTTGGAGCACCAATGCTTTCTCTTATTCAAGTGGAAAGAGTGTTTCTCCCACCGAAGCCAAGAATATACATCTCGGTTTCAGAACCGAGGAAGAATAATCAAGAGGATGAAGAGTCTGAGTCAGTGGGAAAAGAAGAAATAAAGGAGGAGATGAAAGATGAGAAATTGGCCGAAGTGGAAGCTATTCCTCAGTTTAGAATCACTGAAGTCCATGTTGCAGGTCTGAAAACCGAGCCGGATAAAAAGAAGCCATGGGGTACTGCAAACCAGAAGCAGTCCGGTTCCCGCTGGTTGCTTGCTAATGGAATGGGGAAAAGCAATAAGCATCCATTTATGAAGTCAAAAGCTGCTCCAAAATCTTCTGGCCCAGCAACGACAAAGGTGCAGCCTGGTGATACCTTGTGGAGCATCTCAGCACGCGTTCATGGTACTGGAGAAAAATGGAAGGAATTGGCGGCTCTAAATCCACATATAAGGAACCCAAACGTTATTTTCCCGAATGAGACTCTCAGATTAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006810 GO:0006928 GO:0006996 GO:0007097 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009657 GO:0009658 GO:0009902 GO:0009903 GO:0009987 GO:0016043 GO:0019750 GO:0030029 GO:0030048 GO:0030705 GO:0031022 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051644 GO:0051647 GO:0051649 GO:0051656 GO:0051667 GO:0071840 GO:0099515

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LysM NT-C2

Protein Analysis

1120

Amino Acids

122.16

Weight (kDa)

5.28

Isoelectric Point (pI)

49.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NT-C2	PF10358	93 - 241	4e-18	N-terminal C2 in EEIG1 and EHBP1 proteins
PMI1_PMIR1-2_C	PF21745	667 - 818	3.8e-61	PMI1/PMIR1-2, C-terminal domain
LysM	PF01476	1073 - 1118	8.3e-10	LysM domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010745)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20610
fragaria_vesca	FvH4_1g08130
malus_domestica	MD02G1085800.v1.1 MD15G1213000.v1.1
prunus_persica	Prupe.7G203900_v2.0.a1
pyrus_communis	pycom02g06870 pycom02g06880 pycom15g18930 pycom15g18940
rosa_chinensis	RchiOBHm_Chr2g0094181
rosa_laevigata	RLG00000016463
rosa_multiflora	Rmu_sc0002193.1_g000003 Rmu_sc0013176.1_g000002
rosa_roxburghii	Rroxscaffold_2G00147410
rosa_rugosa	Rorug02G0041600 Rorug02G0041700
rosa_samantha	Rh2AG089300 Rh2BG089400 Rh2CG091900 Rh2DG088400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	2002, 3047
Acc65I	GGTACC	1 cut(s)	2032
AccB1I	GGYRCC	1 cut(s)	2032
AccB7I	CCANNNNNTGG	1 cut(s)	1429
AccI	GTMKAC	2 cut(s)	99, 701
AccII	CGCG	1 cut(s)	3258
AclI	AACGTT	1 cut(s)	3324
AclWI	GGATC	4 cut(s)	864, 965, 1511, 2810
AcoI	YGGCCR	1 cut(s)	3006
AcsI	RAATTY	7 cut(s)	10, 428, 923, 1043, 1660, 2207, 2271
AcuI	CTGAAG	5 cut(s)	774, 1380, 1899, 1913, 3063
AfaI	GTAC	6 cut(s)	363, 1167, 1225, 2034, 3097, 3268
AflIII	ACRYGT	2 cut(s)	2472, 3256
AjnI	CCWGG	3 cut(s)	1203, 2484, 3226
Alw21I	GWGCWC	2 cut(s)	544, 2848
Alw26I	GTCTC	7 cut(s)	156, 763, 837, 1530, 1769, 2347, 3335
Alw44I	GTGCAC	1 cut(s)	540
AlwI	GGATC	4 cut(s)	864, 965, 1511, 2810
AlwNI	CAGNNNCTG	1 cut(s)	1448
Ama87I	CYCGRG	1 cut(s)	1520
AoxI	GGCC	9 cut(s)	479, 825, 1815, 1983, 2282, 2590, 2685, 3006, 3202
ApaI	GGGCCC	1 cut(s)	483
ApaLI	GTGCAC	1 cut(s)	540
ApoI	RAATTY	7 cut(s)	10, 428, 923, 1043, 1660, 2207, 2271
AseI	ATTAAT	2 cut(s)	1787, 2585
Asp700I	GAANNNNTTC	3 cut(s)	804, 2783, 3021
Asp718I	GGTACC	1 cut(s)	2032
AspLEI	GCGC	2 cut(s)	2312, 2456
AsuC2I	CCSGG	1 cut(s)	2419
AsuHPI	GGTGA	7 cut(s)	215, 990, 1147, 2519, 2645, 2660, 3242
AvaI	CYCGRG	1 cut(s)	1520
AvaII	GGWCC	5 cut(s)	231, 1419, 1919, 1956, 2195
BaeGI	GKGCMC	2 cut(s)	483, 544
BaeI	ACNNNNGTAYC	2 cut(s)	939, 972
BanI	GGYRCC	1 cut(s)	2032
BanII	GRGCYC	1 cut(s)	483
BauI	CACGAG	1 cut(s)	580
BbsI	GAAGAC	3 cut(s)	1041, 1368, 2358
Bbv12I	GWGCWC	2 cut(s)	544, 2848
BbvCI	CCTCAGC	1 cut(s)	1209
BceAI	ACGGC	1 cut(s)	438
BcgI	CGANNNNNNTGC	6 cut(s)	747, 781, 2417, 2451, 3202, 3236
BciT130I	CCWGG	3 cut(s)	1205, 2486, 3228
BciVI	GTATCC	2 cut(s)	1333, 2403
BclI	TGATCA	2 cut(s)	2004, 2650
BcnI	CCSGG	1 cut(s)	2419
BcoDI	GTCTC	7 cut(s)	156, 763, 837, 1530, 1769, 2347, 3335
BfaI	CTAG	9 cut(s)	155, 551, 897, 1301, 1346, 1475, 1848, 2019, 2757
BfmI	CTRYAG	2 cut(s)	840, 1701
BfuAI	ACCTGC	2 cut(s)	2002, 3047
BfuI	GTATCC	2 cut(s)	1333, 2403
Bme1390I	CCNGG	4 cut(s)	1205, 2419, 2486, 3228
Bme18I	GGWCC	5 cut(s)	231, 1419, 1919, 1956, 2195
BmeT110I	CYCGRG	1 cut(s)	1520
BmiI	GGNNCC	7 cut(s)	164, 481, 482, 1421, 2034, 3121, 3317
BmrFI	CCNGG	4 cut(s)	1205, 2419, 2486, 3228
BmrI	ACTGGG	1 cut(s)	717
BmuI	ACTGGG	1 cut(s)	717
BpiI	GAAGAC	3 cut(s)	1041, 1368, 2358
BplI	GAGNNNNNCTC	6 cut(s)	611, 643, 2327, 2359, 2868, 2900
BpmI	CTGGAG	5 cut(s)	936, 1113, 1593, 2468, 3291
Bpu10I	CCTNAGC	3 cut(s)	628, 1209, 1728
BpuEI	CTTGAG	2 cut(s)	766, 1486
BpuMI	CCSGG	1 cut(s)	2419
BsaBI	GATNNNNATC	1 cut(s)	2649
BsaJI	CCNNGG	7 cut(s)	1203, 2130, 2370, 2485, 2515, 2924, 3089
BsaWI	WCCGGW	3 cut(s)	44, 2630, 3116
BsaXI	ACNNNNNCTCC	4 cut(s)	151, 181, 1935, 1965
Bse118I	RCCGGY	1 cut(s)	2035
Bse3DI	GCAATG	2 cut(s)	2326, 2575
Bse8I	GATNNNNATC	1 cut(s)	2649
BseBI	CCWGG	3 cut(s)	1205, 2486, 3228
BseDI	CCNNGG	7 cut(s)	1203, 2130, 2370, 2485, 2515, 2924, 3089
BseGI	GGATG	8 cut(s)	385, 397, 1438, 2243, 2427, 2544, 2950, 3163
BseJI	GATNNNNATC	1 cut(s)	2649
BseMI	GCAATG	2 cut(s)	2326, 2575
BseRI	GAGGAG	5 cut(s)	620, 629, 632, 1890, 2999
BseSI	GKGCMC	2 cut(s)	483, 544
BseYI	CCCAGC	2 cut(s)	2534, 3205
BsgI	GTGCAG	3 cut(s)	2163, 2237, 3242
Bsh1236I	CGCG	1 cut(s)	3258
BshFI	GGCC	9 cut(s)	481, 827, 1817, 1985, 2284, 2592, 2687, 3008, 3204
BshNI	GGYRCC	1 cut(s)	2032
BsiHKAI	GWGCWC	2 cut(s)	544, 2848
BsiHKCI	CYCGRG	1 cut(s)	1520
BsiSI	CCGG	8 cut(s)	45, 128, 401, 2036, 2418, 2631, 3074, 3117
BslFI	GGGAC	1 cut(s)	1405
BsmAI	GTCTC	7 cut(s)	156, 763, 837, 1530, 1769, 2347, 3335
BsmFI	GGGAC	1 cut(s)	1405
BsnI	GGCC	9 cut(s)	481, 827, 1817, 1985, 2284, 2592, 2687, 3008, 3204
BsoBI	CYCGRG	1 cut(s)	1520
Bsp120I	GGGCCC	1 cut(s)	479
Bsp1286I	GDGCHC	3 cut(s)	483, 544, 2848
Bsp1407I	TGTACA	1 cut(s)	361
Bsp143I	GATC	6 cut(s)	856, 970, 1516, 2004, 2650, 2815
Bsp19I	CCATGG	2 cut(s)	2370, 3089
BspANI	GGCC	9 cut(s)	481, 827, 1817, 1985, 2284, 2592, 2687, 3008, 3204
BspFNI	CGCG	1 cut(s)	3258
BspHI	TCATGA	2 cut(s)	862, 1290
BspLI	GGNNCC	7 cut(s)	164, 481, 482, 1421, 2034, 3121, 3317
BspMAI	CTGCAG	1 cut(s)	1705
BspMI	ACCTGC	2 cut(s)	2002, 3047
BspPI	GGATC	4 cut(s)	864, 965, 1511, 2810
BspQI	GCTCTTC	1 cut(s)	1704
BspT107I	GGYRCC	1 cut(s)	2032
BsrDI	GCAATG	2 cut(s)	2326, 2575
BsrFI	RCCGGY	1 cut(s)	2035
BsrGI	TGTACA	1 cut(s)	361
BssAI	RCCGGY	1 cut(s)	2035
BssECI	CCNNGG	7 cut(s)	1203, 2130, 2370, 2485, 2515, 2924, 3089
BssMI	GATC	6 cut(s)	856, 970, 1516, 2004, 2650, 2815
BssNAI	GTATAC	1 cut(s)	702
BssSI	CACGAG	1 cut(s)	580
BssT1I	CCWWGG	4 cut(s)	2130, 2370, 2515, 3089
Bst1107I	GTATAC	1 cut(s)	702
Bst2BI	CACGAG	1 cut(s)	580
Bst2UI	CCWGG	3 cut(s)	1205, 2486, 3228
Bst4CI	ACNGT	5 cut(s)	167, 281, 706, 2460, 2791
Bst6I	CTCTTC	9 cut(s)	23, 221, 309, 365, 429, 810, 930, 1704, 2943
BstAPI	GCANNNNNTGC	2 cut(s)	517, 2462
BstAUI	TGTACA	1 cut(s)	361
BstC8I	GCNNGC	8 cut(s)	795, 1472, 1603, 1875, 2112, 2317, 3135, 3256
BstDSI	CCRYGG	2 cut(s)	2370, 3089
BstF5I	GGATG	8 cut(s)	385, 397, 1438, 2243, 2427, 2544, 2950, 3163
BstFNI	CGCG	1 cut(s)	3258
BstHHI	GCGC	2 cut(s)	2312, 2456
BstKTI	GATC	6 cut(s)	859, 973, 1519, 2007, 2653, 2818
BstMAI	GTCTC	7 cut(s)	156, 763, 837, 1530, 1769, 2347, 3335
BstMBI	GATC	6 cut(s)	856, 970, 1516, 2004, 2650, 2815
BstNI	CCWGG	3 cut(s)	1205, 2486, 3228
BstNSI	RCATGY	3 cut(s)	456, 1474, 2476
BstSCI	CCNGG	4 cut(s)	1203, 2417, 2484, 3226
BstSFI	CTRYAG	2 cut(s)	840, 1701
BstSLI	GKGCMC	2 cut(s)	483, 544
BstUI	CGCG	1 cut(s)	3258
BstV2I	GAAGAC	3 cut(s)	1041, 1368, 2358
BstX2I	RGATCY	1 cut(s)	856
BstYI	RGATCY	1 cut(s)	856
BstZ17I	GTATAC	1 cut(s)	702
BsuI	GTATCC	2 cut(s)	1333, 2403
BsuRI	GGCC	9 cut(s)	481, 827, 1817, 1985, 2284, 2592, 2687, 3008, 3204
BtgI	CCRYGG	2 cut(s)	2370, 3089
BtsCI	GGATG	8 cut(s)	385, 397, 1438, 2243, 2427, 2544, 2950, 3163
BtsIMutI	CAGTG	8 cut(s)	730, 1158, 1615, 2142, 2562, 2795, 2967, 3039
BveI	ACCTGC	2 cut(s)	2002, 3047
Cac8I	GCNNGC	8 cut(s)	795, 1472, 1603, 1875, 2112, 2317, 3135, 3256
CaiI	CAGNNNCTG	1 cut(s)	1448
CciI	TCATGA	2 cut(s)	862, 1290
CfoI	GCGC	2 cut(s)	2312, 2456
Cfr10I	RCCGGY	1 cut(s)	2035
CseI	GACGC	1 cut(s)	1526
Csp6I	GTAC	6 cut(s)	362, 1166, 1224, 2033, 3096, 3267
CviQI	GTAC	6 cut(s)	362, 1166, 1224, 2033, 3096, 3267
DpnI	GATC	6 cut(s)	858, 972, 1518, 2006, 2652, 2817
DpnII	GATC	6 cut(s)	856, 970, 1516, 2004, 2650, 2815
EaeI	YGGCCR	1 cut(s)	3006
Eam1104I	CTCTTC	9 cut(s)	23, 221, 309, 365, 429, 810, 930, 1704, 2943
EarI	CTCTTC	9 cut(s)	23, 221, 309, 365, 429, 810, 930, 1704, 2943
EciI	GGCGGA	2 cut(s)	51, 1106
Eco130I	CCWWGG	4 cut(s)	2130, 2370, 2515, 3089
Eco147I	AGGCCT	1 cut(s)	827
Eco24I	GRGCYC	1 cut(s)	483
Eco32I	GATATC	1 cut(s)	2140
Eco47I	GGWCC	5 cut(s)	231, 1419, 1919, 1956, 2195
Eco57I	CTGAAG	5 cut(s)	774, 1380, 1899, 1913, 3063
Eco88I	CYCGRG	1 cut(s)	1520
EcoO109I	RGGNCCY	5 cut(s)	231, 480, 1419, 1919, 2195
EcoRI	GAATTC	1 cut(s)	2207
EcoRII	CCWGG	3 cut(s)	1203, 2484, 3226
EcoRV	GATATC	1 cut(s)	2140
EcoT14I	CCWWGG	4 cut(s)	2130, 2370, 2515, 3089
EcoT38I	GRGCYC	1 cut(s)	483
ErhI	CCWWGG	4 cut(s)	2130, 2370, 2515, 3089
FalI	AAGNNNNNCTT	8 cut(s)	218, 250, 973, 1005, 1179, 1211, 2753, 2785
FaqI	GGGAC	1 cut(s)	1405
FauI	CCCGC	4 cut(s)	369, 1785, 1988, 3131
FbaI	TGATCA	2 cut(s)	2004, 2650
FblI	GTMKAC	2 cut(s)	99, 701
FokI	GGATG	8 cut(s)	384, 392, 1445, 2230, 2434, 2551, 2957, 3150
FriOI	GRGCYC	1 cut(s)	483
FspBI	CTAG	9 cut(s)	155, 551, 897, 1301, 1346, 1475, 1848, 2019, 2757
GlaI	GCGC	2 cut(s)	2311, 2455
GsaI	CCCAGC	2 cut(s)	2538, 3209
GsuI	CTGGAG	5 cut(s)	936, 1113, 1593, 2468, 3291
HaeIII	GGCC	9 cut(s)	481, 827, 1817, 1985, 2284, 2592, 2687, 3008, 3204
HapII	CCGG	8 cut(s)	45, 128, 401, 2036, 2418, 2631, 3074, 3117
HgaI	GACGC	1 cut(s)	1526
HhaI	GCGC	2 cut(s)	2312, 2456
Hin6I	GCGC	2 cut(s)	2310, 2454
HinP1I	GCGC	2 cut(s)	2310, 2454
HincII	GTYRAC	3 cut(s)	444, 958, 2578
HindII	GTYRAC	3 cut(s)	444, 958, 2578
HindIII	AAGCTT	1 cut(s)	2099
HpaII	CCGG	8 cut(s)	45, 128, 401, 2036, 2418, 2631, 3074, 3117
HphI	GGTGA	7 cut(s)	215, 990, 1147, 2519, 2645, 2660, 3242
Hpy99I	CGWCG	1 cut(s)	646
HpyCH4III	ACNGT	5 cut(s)	167, 281, 706, 2460, 2791
HpyCH4IV	ACGT	1 cut(s)	3324
HpySE526I	ACGT	1 cut(s)	3324
HspAI	GCGC	2 cut(s)	2310, 2454
KpnI	GGTACC	1 cut(s)	2036
Ksp22I	TGATCA	2 cut(s)	2004, 2650
Kzo9I	GATC	6 cut(s)	856, 970, 1516, 2004, 2650, 2815
LguI	GCTCTTC	1 cut(s)	1704
LmnI	GCTCC	7 cut(s)	2158, 2365, 2528, 2753, 2843, 3193, 3243
MaeI	CTAG	9 cut(s)	155, 551, 897, 1301, 1346, 1475, 1848, 2019, 2757
MaeII	ACGT	1 cut(s)	3324
MaeIII	GTNAC	6 cut(s)	137, 391, 819, 1105, 1438, 1610
MalI	GATC	6 cut(s)	858, 972, 1518, 2006, 2652, 2817
MboI	GATC	6 cut(s)	856, 970, 1516, 2004, 2650, 2815
MfeI	CAATTG	1 cut(s)	2394
MflI	RGATCY	1 cut(s)	856
MhlI	GDGCHC	3 cut(s)	483, 544, 2848
MluI	ACGCGT	1 cut(s)	3256
MlyI	GAGTC	9 cut(s)	439, 1256, 1313, 1388, 1532, 1655, 2960, 2966, 3337
MmeI	TCCRAC	4 cut(s)	867, 1281, 1743, 2821
MroXI	GAANNNNTTC	3 cut(s)	804, 2783, 3021
MseI	TTAA	9 cut(s)	215, 288, 1787, 1805, 2123, 2300, 2585, 2600, 3353
MspA1I	CMGCKG	3 cut(s)	1133, 2315, 3126
MspI	CCGG	8 cut(s)	45, 128, 401, 2036, 2418, 2631, 3074, 3117
MspR9I	CCNGG	4 cut(s)	1205, 2419, 2486, 3228
MunI	CAATTG	1 cut(s)	2394
MvaI	CCWGG	3 cut(s)	1205, 2486, 3228
MvnI	CGCG	1 cut(s)	3258
NciI	CCSGG	1 cut(s)	2419
NcoI	CCATGG	2 cut(s)	2370, 3089
NdeII	GATC	6 cut(s)	856, 970, 1516, 2004, 2650, 2815
NlaIV	GGNNCC	7 cut(s)	164, 481, 482, 1421, 2034, 3121, 3317
NmuCI	GTSAC	2 cut(s)	819, 1610
NspI	RCATGY	3 cut(s)	456, 1474, 2476
PaeI	GCATGC	1 cut(s)	1474
PagI	TCATGA	2 cut(s)	862, 1290
PceI	AGGCCT	1 cut(s)	827
PciI	ACATGT	1 cut(s)	2472
PciSI	GCTCTTC	1 cut(s)	1704
PdmI	GAANNNNTTC	3 cut(s)	804, 2783, 3021
PflMI	CCANNNNNTGG	1 cut(s)	1429
PleI	GAGTC	9 cut(s)	439, 1255, 1312, 1388, 1531, 1654, 2959, 2965, 3337
PpsI	GAGTC	9 cut(s)	439, 1255, 1312, 1388, 1531, 1654, 2959, 2965, 3337
PpuMI	RGGWCCY	4 cut(s)	231, 1419, 1919, 2195
PscI	ACATGT	1 cut(s)	2472
PshBI	ATTAAT	2 cut(s)	1787, 2585
Psp1406I	AACGTT	1 cut(s)	3324
Psp5II	RGGWCCY	4 cut(s)	231, 1419, 1919, 2195
Psp6I	CCWGG	3 cut(s)	1203, 2484, 3226
PspFI	CCCAGC	2 cut(s)	2534, 3205
PspGI	CCWGG	3 cut(s)	1203, 2484, 3226
PspN4I	GGNNCC	7 cut(s)	164, 481, 482, 1421, 2034, 3121, 3317
PspOMI	GGGCCC	1 cut(s)	479
PspPPI	RGGWCCY	4 cut(s)	231, 1419, 1919, 2195
PstI	CTGCAG	1 cut(s)	1705
PstNI	CAGNNNCTG	1 cut(s)	1448
PsuI	RGATCY	1 cut(s)	856
PvuII	CAGCTG	2 cut(s)	1133, 2315
RsaI	GTAC	6 cut(s)	363, 1167, 1225, 2034, 3097, 3268
RsaNI	GTAC	6 cut(s)	362, 1166, 1224, 2033, 3096, 3267
SapI	GCTCTTC	1 cut(s)	1704
SaqAI	TTAA	9 cut(s)	215, 288, 1787, 1805, 2123, 2300, 2585, 2600, 3353
Sau3AI	GATC	6 cut(s)	856, 970, 1516, 2004, 2650, 2815
SchI	GAGTC	9 cut(s)	439, 1256, 1313, 1388, 1532, 1655, 2960, 2966, 3337
ScrFI	CCNGG	4 cut(s)	1205, 2419, 2486, 3228
SduI	GDGCHC	3 cut(s)	483, 544, 2848
SfcI	CTRYAG	2 cut(s)	840, 1701
SinI	GGWCC	5 cut(s)	231, 1419, 1919, 1956, 2195
SmlI	CTYRAG	2 cut(s)	781, 1465
SmoI	CTYRAG	2 cut(s)	781, 1465
SphI	GCATGC	1 cut(s)	1474
SseBI	AGGCCT	1 cut(s)	827
SspMI	CTAG	9 cut(s)	155, 551, 897, 1301, 1346, 1475, 1848, 2019, 2757
StuI	AGGCCT	1 cut(s)	827
StyD4I	CCNGG	4 cut(s)	1203, 2417, 2484, 3226
StyI	CCWWGG	4 cut(s)	2130, 2370, 2515, 3089
TaaI	ACNGT	5 cut(s)	167, 281, 706, 2460, 2791
TaiI	ACGT	1 cut(s)	3327
TaqI	TCGA	4 cut(s)	185, 767, 1397, 2306
TatI	WGTACW	1 cut(s)	361
TauI	GCSGC	2 cut(s)	2687, 3296
Tru1I	TTAA	9 cut(s)	215, 288, 1787, 1805, 2123, 2300, 2585, 2600, 3353
Tru9I	TTAA	9 cut(s)	215, 288, 1787, 1805, 2123, 2300, 2585, 2600, 3353
TscAI	CASTG	8 cut(s)	730, 1165, 1615, 2149, 2569, 2802, 2967, 3046
TseFI	GTSAC	2 cut(s)	819, 1610
Tsp45I	GTSAC	2 cut(s)	819, 1610
TspGWI	ACGGA	2 cut(s)	2595, 2680
TspRI	CASTG	8 cut(s)	730, 1165, 1615, 2149, 2569, 2802, 2967, 3046
Van91I	CCANNNNNTGG	1 cut(s)	1429
VneI	GTGCAC	1 cut(s)	540
VpaK11BI	GGWCC	5 cut(s)	231, 1419, 1919, 1956, 2195
VspI	ATTAAT	2 cut(s)	1787, 2585
XapI	RAATTY	7 cut(s)	10, 428, 923, 1043, 1660, 2207, 2271
XceI	RCATGY	3 cut(s)	456, 1474, 2476
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1234, 1622
XmiI	GTMKAC	2 cut(s)	99, 701
XmnI	GAANNNNTTC	3 cut(s)	804, 2783, 3021
XspI	CTAG	9 cut(s)	155, 551, 897, 1301, 1346, 1475, 1848, 2019, 2757

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.