Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0014425

Physical Location & Seq

Genomic Coordinates Forward (+)

12557394 .. 12558377

Gene Structure 984 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1152200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 984 bp

ATGAGAGTGCCTGTTTCTTCCCCACACAGCAACAACAACAACCAATCTCCCAAGCACAGCACCCAGACTAATAACCAAAGTGTCAACTTTCACCTAGGTATTTCGAACTTGGGGGGCTACGAGCCCACCTCGGTTCTCGACCTCCGCCACAGCCCCAGCCCTGTCTCCGCCGAGAAACCCTCCAAAATTTTGGCAATCTCCGACGTGCTGTCCCACTCGAACCATGACCCTGTGGAGTGGGAGGAGCAAGTGGACTGGGACTCAATCATGAAAGACTTAGGGTTGCACGACGACAACGTCCCGAACCTCAAGAATTCAATCCCGCAATTGAATTCAAACGATCCTCAGATTTCGGAGTACCTGCATCCTCAACCGTTCGATCCCACCCAGCTCGTCCACTCCGATTACAGCATCAATCTCTCCGAAGTGTACTCCTCCCAGAATTGTTACGACCATGCGATCAACGACTTCCATCAATCGGGCAACTGGAACGTAGGGGTTGATTTCATAGAGGAATTAATCCGCGCCGCGGACTGTTTCGACTCGGACGAGTTGCAACTCGCTCAGGTGATCCTAGACCGTTTGAACAACAGGATGCGATCCTCCTCCCCGTCGAAACCCGTCGGAAAGCCACTCCAGCGCGCCGCGGTTTATTTCAAGGACGCTCTTCAATCCCTCCTCAATGGCTCTGATACGGCGGCGCGCGAGTTCTCTTCCTCGTCCGACATCGTCCATACAATCCGCGCCTACAAGAGCTTTTCCGGAATTTCGCCGGTCCCGATGTTCTCTCACTTCACAACAAACCAGGCCCTGCTCGAAGCCCTCAACGGATCAGCCTTCATCCACGTCATCGACTTTGACATCGGCCTGGGCGGCCAGTACGCTTCCCTAATGAAAGAGCTCGCCGAGAAAGCTGACGTGGCGAGCCGAAATAAAGGCAACCAGGTCCCACCGGTGCTCCGCATCACCGCCGTCGTGCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048831 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000026 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

328

Amino Acids

36.11

Weight (kDa)

5.26

Isoelectric Point (pI)

45.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	171 - 326	2.8e-38	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36710
fragaria_vesca	FvH4_1g13740
malus_domestica	MD02G1152200.v1.1 MD15G1266500.v1.1
prunus_persica	Prupe.7G150900_v2.0.a1
pyrus_communis	pycom02g11950
rosa_chinensis	RchiOBHm_Chr2g0102171
rosa_laevigata	RLG00000017134
rosa_multiflora	Rmu_co8491613.1_g000001 Rmu_sc0000157.1_g000043
rosa_roxburghii	Rroxscaffold_2G00140730
rosa_rugosa	Rorug02G0104800
rosa_samantha	Rh2AG153200 Rh2BG159100 Rh2CG158600 Rh2DG158800
rosa_wichuraiana	Rw2G011960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	296
Acc36I	ACCTGC	1 cut(s)	369
AccII	CGCG	7 cut(s)	525, 530, 642, 647, 703, 705, 744
AccIII	TCCGGA	1 cut(s)	761
AclWI	GGATC	5 cut(s)	335, 374, 565, 594, 838
AcoI	YGGCCR	1 cut(s)	874
AcsI	RAATTY	4 cut(s)	186, 313, 331, 765
AfaI	GTAC	3 cut(s)	359, 431, 881
AfiI	CCNNNNNNNGG	2 cut(s)	478, 529
AgeI	ACCGGT	1 cut(s)	952
AgsI	TTSAA	6 cut(s)	318, 331, 336, 586, 658, 671
AhdI	GACNNNNNGTC	1 cut(s)	208
AjiI	CACGTC	3 cut(s)	205, 847, 919
AjnI	CCWGG	3 cut(s)	804, 867, 942
AluBI	AGCT	4 cut(s)	391, 756, 901, 914
AluI	AGCT	4 cut(s)	391, 756, 901, 914
Alw21I	GWGCWC	2 cut(s)	903, 960
Alw26I	GTCTC	1 cut(s)	169
AlwI	GGATC	5 cut(s)	335, 374, 565, 594, 838
AlwNI	CAGNNNCTG	1 cut(s)	811
Aor13HI	TCCGGA	1 cut(s)	761
AoxI	GGCC	3 cut(s)	807, 865, 874
ApoI	RAATTY	4 cut(s)	186, 313, 331, 765
AseI	ATTAAT	1 cut(s)	518
AsiGI	ACCGGT	1 cut(s)	952
AspA2I	CCTAGG	1 cut(s)	94
AspLEI	GCGC	6 cut(s)	527, 642, 644, 703, 705, 746
AspS9I	GGNCC	3 cut(s)	775, 808, 946
AsuHPI	GGTGA	3 cut(s)	83, 580, 958
AsuII	TTCGAA	1 cut(s)	104
AvaII	GGWCC	2 cut(s)	775, 946
AvrII	CCTAGG	1 cut(s)	94
BanII	GRGCYC	2 cut(s)	126, 903
Bbv12I	GWGCWC	2 cut(s)	903, 960
BccI	CCATC	1 cut(s)	480
BceAI	ACGGC	2 cut(s)	711, 956
BciT130I	CCWGG	3 cut(s)	806, 869, 944
BcoDI	GTCTC	1 cut(s)	169
BfaI	CTAG	2 cut(s)	95, 575
BfuAI	ACCTGC	1 cut(s)	369
BglI	GCCNNNNNGGC	1 cut(s)	873
BisI	GCNGC	4 cut(s)	528, 645, 699, 874
BlnI	CCTAGG	1 cut(s)	94
BlsI	GCNGC	4 cut(s)	529, 646, 700, 875
Bme1390I	CCNGG	3 cut(s)	806, 869, 944
Bme18I	GGWCC	2 cut(s)	775, 946
BmeRI	GACNNNNNGTC	1 cut(s)	208
BmgBI	CACGTC	3 cut(s)	205, 847, 919
BmgT120I	GGNCC	3 cut(s)	775, 808, 946
BmiI	GGNNCC	2 cut(s)	777, 948
BmrFI	CCNGG	3 cut(s)	806, 869, 944
BmrI	ACTGGG	1 cut(s)	265
BmsI	GCATC	4 cut(s)	373, 420, 585, 972
BmuI	ACTGGG	1 cut(s)	265
BplI	GAGNNNNNCTC	4 cut(s)	113, 145, 164, 196
BpmI	CTGGAG	1 cut(s)	620
Bpu10I	CCTNAGC	1 cut(s)	564
Bpu14I	TTCGAA	1 cut(s)	104
BpuEI	CTTGAG	1 cut(s)	293
BsaJI	CCNNGG	5 cut(s)	94, 129, 528, 645, 868
BsaWI	WCCGGW	2 cut(s)	761, 952
BsaXI	ACNNNNNCTCC	2 cut(s)	942, 972
Bsc4I	CCNNNNNNNGG	2 cut(s)	478, 529
Bse118I	RCCGGY	2 cut(s)	772, 952
Bse1I	ACTGG	3 cut(s)	260, 491, 877
BseAI	TCCGGA	1 cut(s)	761
BseBI	CCWGG	3 cut(s)	806, 869, 944
BseDI	CCNNGG	5 cut(s)	94, 129, 528, 645, 868
BseGI	GGATG	3 cut(s)	364, 600, 840
BseLI	CCNNNNNNNGG	2 cut(s)	478, 529
BseMII	CTCAG	2 cut(s)	359, 578
BseNI	ACTGG	3 cut(s)	260, 491, 877
BsePI	GCGCGC	2 cut(s)	640, 701
BseRI	GAGGAG	4 cut(s)	257, 424, 595, 668
BseYI	CCCAGC	2 cut(s)	155, 387
Bsh1236I	CGCG	7 cut(s)	525, 530, 642, 647, 703, 705, 744
BshFI	GGCC	3 cut(s)	809, 867, 876
BshTI	ACCGGT	1 cut(s)	952
BsiHKAI	GWGCWC	2 cut(s)	903, 960
BsiSI	CCGG	3 cut(s)	762, 773, 953
BslFI	GGGAC	5 cut(s)	196, 272, 284, 761, 932
BslI	CCNNNNNNNGG	2 cut(s)	478, 529
BsmAI	GTCTC	1 cut(s)	169
BsmFI	GGGAC	5 cut(s)	196, 272, 284, 761, 932
BsnI	GGCC	3 cut(s)	809, 867, 876
Bsp119I	TTCGAA	1 cut(s)	104
Bsp1286I	GDGCHC	3 cut(s)	126, 903, 960
Bsp13I	TCCGGA	1 cut(s)	761
Bsp143I	GATC	6 cut(s)	340, 379, 459, 570, 599, 830
BspANI	GGCC	3 cut(s)	809, 867, 876
BspCNI	CTCAG	2 cut(s)	358, 577
BspEI	TCCGGA	1 cut(s)	761
BspFNI	CGCG	7 cut(s)	525, 530, 642, 647, 703, 705, 744
BspHI	TCATGA	1 cut(s)	267
BspLI	GGNNCC	2 cut(s)	777, 948
BspMI	ACCTGC	1 cut(s)	369
BspPI	GGATC	5 cut(s)	335, 374, 565, 594, 838
BspQI	GCTCTTC	1 cut(s)	672
BspT104I	TTCGAA	1 cut(s)	104
BsrFI	RCCGGY	2 cut(s)	772, 952
BsrI	ACTGG	3 cut(s)	260, 491, 877
BssAI	RCCGGY	2 cut(s)	772, 952
BssECI	CCNNGG	5 cut(s)	94, 129, 528, 645, 868
BssHII	GCGCGC	2 cut(s)	640, 701
BssMI	GATC	6 cut(s)	340, 379, 459, 570, 599, 830
BssT1I	CCWWGG	1 cut(s)	94
Bst2UI	CCWGG	3 cut(s)	806, 869, 944
Bst4CI	ACNGT	3 cut(s)	375, 536, 581
Bst6I	CTCTTC	2 cut(s)	672, 718
BstBI	TTCGAA	1 cut(s)	104
BstC8I	GCNNGC	4 cut(s)	642, 703, 903, 925
BstDEI	CTNAG	3 cut(s)	277, 345, 564
BstDSI	CCRYGG	2 cut(s)	528, 645
BstF5I	GGATG	3 cut(s)	364, 600, 840
BstFNI	CGCG	7 cut(s)	525, 530, 642, 647, 703, 705, 744
BstHHI	GCGC	6 cut(s)	527, 642, 644, 703, 705, 746
BstKTI	GATC	6 cut(s)	343, 382, 462, 573, 602, 833
BstMAI	GTCTC	1 cut(s)	169
BstMBI	GATC	6 cut(s)	340, 379, 459, 570, 599, 830
BstMWI	GCNNNNNNNGC	4 cut(s)	637, 873, 911, 920
BstNI	CCWGG	3 cut(s)	806, 869, 944
BstSCI	CCNGG	3 cut(s)	804, 867, 942
BstUI	CGCG	7 cut(s)	525, 530, 642, 647, 703, 705, 744
BstXI	CCANNNNNNTGG	1 cut(s)	190
BsuRI	GGCC	3 cut(s)	809, 867, 876
BtgI	CCRYGG	2 cut(s)	528, 645
BtrI	CACGTC	3 cut(s)	205, 847, 919
BtsCI	GGATG	3 cut(s)	364, 600, 840
BveI	ACCTGC	1 cut(s)	369
Cac8I	GCNNGC	4 cut(s)	642, 703, 903, 925
CaiI	CAGNNNCTG	1 cut(s)	811
CciI	TCATGA	1 cut(s)	267
CfoI	GCGC	6 cut(s)	527, 642, 644, 703, 705, 746
Cfr10I	RCCGGY	2 cut(s)	772, 952
Cfr13I	GGNCC	3 cut(s)	775, 808, 946
Cfr42I	CCGCGG	2 cut(s)	531, 648
CseI	GACGC	1 cut(s)	671
CsiI	ACCWGGT	1 cut(s)	942
Csp6I	GTAC	3 cut(s)	358, 430, 880
CspAI	ACCGGT	1 cut(s)	952
CviAII	CATG	4 cut(s)	224, 268, 455, 981
CviQI	GTAC	3 cut(s)	358, 430, 880
DdeI	CTNAG	3 cut(s)	277, 345, 564
DpnI	GATC	6 cut(s)	342, 381, 461, 572, 601, 832
DpnII	GATC	6 cut(s)	340, 379, 459, 570, 599, 830
DrdI	GACNNNNNNGTC	1 cut(s)	296
DriI	GACNNNNNGTC	1 cut(s)	208
DseDI	GACNNNNNNGTC	1 cut(s)	296
EaeI	YGGCCR	1 cut(s)	874
Eam1104I	CTCTTC	2 cut(s)	672, 718
Eam1105I	GACNNNNNGTC	1 cut(s)	208
EarI	CTCTTC	2 cut(s)	672, 718
EciI	GGCGGA	2 cut(s)	134, 157
Ecl136II	GAGCTC	1 cut(s)	901
Eco130I	CCWWGG	1 cut(s)	94
Eco24I	GRGCYC	2 cut(s)	126, 903
Eco47I	GGWCC	2 cut(s)	775, 946
Eco53kI	GAGCTC	1 cut(s)	901
EcoICRI	GAGCTC	1 cut(s)	901
EcoO109I	RGGNCCY	2 cut(s)	808, 946
EcoRI	GAATTC	2 cut(s)	313, 331
EcoRII	CCWGG	3 cut(s)	804, 867, 942
EcoT14I	CCWWGG	1 cut(s)	94
EcoT38I	GRGCYC	2 cut(s)	126, 903
ErhI	CCWWGG	1 cut(s)	94
FaeI	CATG	4 cut(s)	227, 271, 458, 984
FaiI	YATR	6 cut(s)	225, 269, 456, 509, 735, 982
FaqI	GGGAC	5 cut(s)	196, 272, 284, 761, 932
FatI	CATG	4 cut(s)	223, 267, 454, 980
FauI	CCCGC	1 cut(s)	330
Fnu4HI	GCNGC	4 cut(s)	528, 645, 699, 874
FokI	GGATG	3 cut(s)	351, 607, 827
FriOI	GRGCYC	2 cut(s)	126, 903
Fsp4HI	GCNGC	4 cut(s)	528, 645, 699, 874
FspBI	CTAG	2 cut(s)	95, 575
GlaI	GCGC	6 cut(s)	526, 641, 643, 702, 704, 745
GluI	GCNGC	4 cut(s)	528, 645, 699, 874
GsaI	CCCAGC	2 cut(s)	159, 391
GsuI	CTGGAG	1 cut(s)	620
HaeIII	GGCC	3 cut(s)	809, 867, 876
HapII	CCGG	3 cut(s)	762, 773, 953
HgaI	GACGC	1 cut(s)	671
HhaI	GCGC	6 cut(s)	527, 642, 644, 703, 705, 746
Hin1II	CATG	4 cut(s)	227, 271, 458, 984
Hin6I	GCGC	6 cut(s)	525, 640, 642, 701, 703, 744
HinP1I	GCGC	6 cut(s)	525, 640, 642, 701, 703, 744
HincII	GTYRAC	1 cut(s)	85
HindII	GTYRAC	1 cut(s)	85
HinfI	GANTC	2 cut(s)	260, 542
HpaII	CCGG	3 cut(s)	762, 773, 953
HphI	GGTGA	3 cut(s)	83, 580, 958
Hpy166II	GTNNAC	4 cut(s)	85, 253, 397, 430
Hpy188I	TCNGA	9 cut(s)	202, 348, 355, 403, 424, 547, 626, 691, 724
Hpy188III	TCNNGA	6 cut(s)	137, 268, 301, 310, 762, 778
Hpy8I	GTNNAC	4 cut(s)	85, 253, 397, 430
Hpy99I	CGWCG	5 cut(s)	206, 293, 616, 626, 977
HpyAV	CCTTC	1 cut(s)	847
HpyCH4III	ACNGT	3 cut(s)	375, 536, 581
HpyCH4IV	ACGT	5 cut(s)	204, 297, 492, 846, 918
HpyCH4V	TGCA	3 cut(s)	286, 364, 556
HpyF10VI	GCNNNNNNNGC	4 cut(s)	637, 873, 911, 920
HpyF3I	CTNAG	3 cut(s)	277, 345, 564
HpySE526I	ACGT	5 cut(s)	204, 297, 492, 846, 918
Hsp92II	CATG	4 cut(s)	227, 271, 458, 984
HspAI	GCGC	6 cut(s)	525, 640, 642, 701, 703, 744
Kpn2I	TCCGGA	1 cut(s)	761
KspI	CCGCGG	2 cut(s)	531, 648
Kzo9I	GATC	6 cut(s)	340, 379, 459, 570, 599, 830
LguI	GCTCTTC	1 cut(s)	672
LmnI	GCTCC	2 cut(s)	244, 963
LweI	GCATC	4 cut(s)	373, 420, 585, 972
MabI	ACCWGGT	1 cut(s)	942
MaeI	CTAG	2 cut(s)	95, 575
MaeII	ACGT	5 cut(s)	204, 297, 492, 846, 918
MaeIII	GTNAC	1 cut(s)	446
MalI	GATC	6 cut(s)	342, 381, 461, 572, 601, 832
MboI	GATC	6 cut(s)	340, 379, 459, 570, 599, 830
MboII	GAAGA	3 cut(s)	9, 659, 705
MfeI	CAATTG	1 cut(s)	326
MhlI	GDGCHC	3 cut(s)	126, 903, 960
MluCI	AATT	7 cut(s)	186, 313, 326, 331, 442, 515, 765
MlyI	GAGTC	2 cut(s)	254, 536
MmeI	TCCRAC	3 cut(s)	225, 604, 747
MroI	TCCGGA	1 cut(s)	761
MseI	TTAA	1 cut(s)	518
MspA1I	CMGCKG	2 cut(s)	530, 647
MspI	CCGG	3 cut(s)	762, 773, 953
MspR9I	CCNGG	3 cut(s)	806, 869, 944
MunI	CAATTG	1 cut(s)	326
MvaI	CCWGG	3 cut(s)	806, 869, 944
MvnI	CGCG	7 cut(s)	525, 530, 642, 647, 703, 705, 744
MwoI	GCNNNNNNNGC	4 cut(s)	637, 873, 911, 920
NdeII	GATC	6 cut(s)	340, 379, 459, 570, 599, 830
NlaIII	CATG	4 cut(s)	227, 271, 458, 984
NlaIV	GGNNCC	2 cut(s)	777, 948
NmeAIII	GCCGAG	2 cut(s)	196, 931
NspV	TTCGAA	1 cut(s)	104
PagI	TCATGA	1 cut(s)	267
PauI	GCGCGC	2 cut(s)	640, 701
PciSI	GCTCTTC	1 cut(s)	672
PcsI	WCGNNNNNNNCGW	4 cut(s)	294, 399, 546, 776
PflFI	GACNNNGTC	2 cut(s)	296, 728
PinAI	ACCGGT	1 cut(s)	952
PkrI	GCNGC	4 cut(s)	529, 646, 700, 875
PleI	GAGTC	2 cut(s)	254, 536
PpsI	GAGTC	2 cut(s)	254, 536
PpuMI	RGGWCCY	1 cut(s)	946
PshBI	ATTAAT	1 cut(s)	518
Psp124BI	GAGCTC	1 cut(s)	903
Psp5II	RGGWCCY	1 cut(s)	946
Psp6I	CCWGG	3 cut(s)	804, 867, 942
PspFI	CCCAGC	2 cut(s)	155, 387
PspGI	CCWGG	3 cut(s)	804, 867, 942
PspN4I	GGNNCC	2 cut(s)	777, 948
PspPI	GGNCC	3 cut(s)	775, 808, 946
PspPPI	RGGWCCY	1 cut(s)	946
PstNI	CAGNNNCTG	1 cut(s)	811
PsyI	GACNNNGTC	2 cut(s)	296, 728
PteI	GCGCGC	2 cut(s)	640, 701
RsaI	GTAC	3 cut(s)	359, 431, 881
RsaNI	GTAC	3 cut(s)	358, 430, 880
SacI	GAGCTC	1 cut(s)	903
SacII	CCGCGG	2 cut(s)	531, 648
SapI	GCTCTTC	1 cut(s)	672
SaqAI	TTAA	1 cut(s)	518
SatI	GCNGC	4 cut(s)	528, 645, 699, 874
Sau3AI	GATC	6 cut(s)	340, 379, 459, 570, 599, 830
Sau96I	GGNCC	3 cut(s)	775, 808, 946
SchI	GAGTC	2 cut(s)	254, 536
ScrFI	CCNGG	3 cut(s)	806, 869, 944
SduI	GDGCHC	3 cut(s)	126, 903, 960
SexAI	ACCWGGT	1 cut(s)	942
SfaNI	GCATC	4 cut(s)	373, 420, 585, 972
SfiI	GGCCNNNNNGGCC	1 cut(s)	873
Sfr303I	CCGCGG	2 cut(s)	531, 648
SfuI	TTCGAA	1 cut(s)	104
SgrAI	CRCCGGYG	1 cut(s)	952
SgrBI	CCGCGG	2 cut(s)	531, 648
SinI	GGWCC	2 cut(s)	775, 946
SmlI	CTYRAG	1 cut(s)	308
SmoI	CTYRAG	1 cut(s)	308
Sse9I	AATT	7 cut(s)	186, 313, 326, 331, 442, 515, 765
SspMI	CTAG	2 cut(s)	95, 575
SstI	GAGCTC	1 cut(s)	903
StyD4I	CCNGG	3 cut(s)	804, 867, 942
StyI	CCWWGG	1 cut(s)	94
TaaI	ACNGT	3 cut(s)	375, 536, 581
TaiI	ACGT	5 cut(s)	207, 300, 495, 849, 921
TaqI	TCGA	8 cut(s)	104, 138, 218, 378, 540, 614, 816, 852
TasI	AATT	7 cut(s)	186, 313, 326, 331, 442, 515, 765
TatI	WGTACW	1 cut(s)	429
TauI	GCSGC	4 cut(s)	530, 647, 701, 876
Tru1I	TTAA	1 cut(s)	518
Tru9I	TTAA	1 cut(s)	518
TspDTI	ATGAA	4 cut(s)	284, 496, 829, 908
TspGWI	ACGGA	1 cut(s)	843
Tth111I	GACNNNGTC	2 cut(s)	296, 728
VpaK11BI	GGWCC	2 cut(s)	775, 946
VspI	ATTAAT	1 cut(s)	518
XapI	RAATTY	4 cut(s)	186, 313, 331, 765
XmaJI	CCTAGG	1 cut(s)	94
XspI	CTAG	2 cut(s)	95, 575

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.