MD02G1155400.v1.1

Belongs to the glycosyltransferase 8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013971
Physical Location & Seq
Forward (+)
12880492 .. 12881562
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1155400.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGCTCTCTTTCCTCATCAGCTTCATCCTTCTCTTTACGACCACCACCAATGCCACCATCACTACTTCCGAACGACTCAGGGAAGCGCTCGCCACCGCAACCACTCAACGATTCAAGGAAGCACCGAAATTCTACAACTCCCCCAGCTGCCCGGACCTCAACAACCACGAGCACACAGCGGTGGCCATGTGCTCGGATGAGGCGGTCCACGTGGCAATGACCCTCGACGCCGCTTACCTCCGTGGCTCAATGGCCGCCATTCTCTCCGTCCTCCAACACTCCTCCTGCCCGGAAAATGTCATCTTCCACTTCGTCTCCTCCTCCTCCACCTCCAACTCCCAACTCCTCCAGCAAATTATTGCGACGTCATTTCCCTACTTGAAATTCCGAGTCTACCCCTTCGACGACTCGGCCGTGGCGGGGCTCATATCGACGTCGATCCGCTCCGCCCTCGACTGCCCGCTCAACTACGCTCGCAACTACCTCGCAAACATCCTCCCCACATGTGTCCGCCGAGTCGTCTACCTCGACTCCGACCTCATCCTCGTCGACGACATTTCCAAGCTGGCGTCCACCCCGCTGGGCCCCACCCAAGTCCTCGCCGCGCCGGAGTACTGCAACGCCAACTTCTCCTCCTACTTTACGCCCACGTTTTGGTCCAACCCCACGCTCTCCCTCACCTTCGAGAACCGCAAGGCCTGCTACTTCAACACCGGCGTCATGGTCATCGACTTGGACAAGTGGAGATCCGGTGGGTACACGGAACGGATCGTGGAGTGGATGGAGCTACAGAAGCGGATGCGGATCTACGAGCTCGGGTCCCTCCCGCCTTTCCTATTGGTTTTCGCCGGAAACATCGCCGCAGTCGACCACCGATGGAACCAGCACGGACTGGGCGGAGATAACTTTCGTGGGTTGTGTCGGGATTTGCATCCGGGTCCGGTGAGCTTGCTGCATTGGAGCGGGAAGGGGAAGCCGTGGGTGAGACTGGACGCCAACCGACCGTGCCCATTGGATGCTCTGTGGGGCCCATATGATCTGTTACAGACACCCTTCGCGCTGGAGTCTTGA

Protein Analysis

357

Amino Acids

39.75

Weight (kDa)

6.3

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_8 PF01501 70 - 329 1.2e-50 Glycosyl transferase family 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013971)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50760
fragaria_vesca FvH4_1g14120
malus_domestica MD02G1155400.v1.1 MD15G1269900.v1.1
prunus_persica Prupe.7G148000_v2.0.a1
pyrus_communis pycom02g12300 pycom15g23400
rosa_chinensis RchiOBHm_Chr2g0102811
rosa_laevigata RLG00000017180
rosa_multiflora Rmu_sc0006537.1_g000025
rosa_roxburghii Rroxscaffold_2G00140310
rosa_rugosa Rorug02G0109500
rosa_samantha Rh2AG158000 Rh2BG164500 Rh2CG164200 Rh2DG163300
rosa_wichuraiana Rw2G012280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 368, 437
AccB7I CCANNNNNTGG 1 cut(s) 654
AccBSI CCGCTC 3 cut(s) 444, 463, 963
AccI GTMKAC 4 cut(s) 393, 522, 549, 867
AccII CGCG 2 cut(s) 605, 1058
AclWI GGATC 4 cut(s) 433, 741, 776, 812
AcoI YGGCCR 3 cut(s) 183, 252, 411
AcsI RAATTY 2 cut(s) 128, 383
AcvI CACGTG 1 cut(s) 211
AcyI GRCGYC 6 cut(s) 228, 365, 434, 569, 717, 993
AfaI GTAC 2 cut(s) 614, 758
AfeI AGCGCT 1 cut(s) 87
AfiI CCNNNNNNNGG 2 cut(s) 420, 654
AflIII ACRYGT 1 cut(s) 503
AgsI TTSAA 3 cut(s) 115, 382, 709
AleI CACNNNNGTG 1 cut(s) 179
AluBI AGCT 6 cut(s) 21, 147, 565, 787, 814, 948
AluI AGCT 6 cut(s) 21, 147, 565, 787, 814, 948
Alw21I GWGCWC 3 cut(s) 174, 194, 816
Alw26I GTCTC 2 cut(s) 319, 979
AlwI GGATC 4 cut(s) 433, 741, 776, 812
Ama87I CYCGRG 1 cut(s) 815
Aor51HI AGCGCT 1 cut(s) 87
AoxI GGCC 6 cut(s) 183, 252, 411, 583, 696, 1027
ApaI GGGCCC 2 cut(s) 587, 1031
ApeKI GCWGC 2 cut(s) 147, 952
ApoI RAATTY 2 cut(s) 128, 383
AspLEI GCGC 3 cut(s) 88, 607, 1060
AspS9I GGNCC 9 cut(s) 154, 205, 583, 584, 657, 819, 938, 1027, 1028
AsuC2I CCSGG 3 cut(s) 152, 290, 936
AsuHPI GGTGA 3 cut(s) 670, 955, 994
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 5 cut(s) 154, 205, 657, 819, 938
BaeGI GKGCMC 3 cut(s) 587, 1010, 1031
BalI TGGCCA 1 cut(s) 185
BanII GRGCYC 4 cut(s) 426, 587, 816, 1031
BauI CACGAG 1 cut(s) 167
BbrPI CACGTG 1 cut(s) 211
Bbv12I GWGCWC 3 cut(s) 174, 194, 816
BbvI GCAGC 2 cut(s) 134, 939
BccI CCATC 3 cut(s) 65, 775, 870
BceAI ACGGC 2 cut(s) 398, 961
BcgI CGANNNNNNTGC 2 cut(s) 466, 500
BcnI CCSGG 3 cut(s) 152, 290, 936
BcoDI GTCTC 2 cut(s) 319, 979
BfmI CTRYAG 1 cut(s) 788
BfoI RGCGCY 1 cut(s) 89
BisI GCNGC 6 cut(s) 148, 231, 255, 603, 861, 953
BlsI GCNGC 6 cut(s) 149, 232, 256, 604, 862, 954
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 3 cut(s) 152, 290, 936
Bme18I GGWCC 5 cut(s) 154, 205, 657, 819, 938
BmeT110I CYCGRG 1 cut(s) 815
BmgT120I GGNCC 9 cut(s) 154, 205, 583, 584, 657, 819, 938, 1027, 1028
BmiI GGNNCC 8 cut(s) 585, 586, 820, 821, 881, 939, 1028, 1029
BmrFI CCNGG 3 cut(s) 152, 290, 936
BmrI ACTGGG 1 cut(s) 902
BmsI GCATC 3 cut(s) 789, 940, 1006
BmuI ACTGGG 1 cut(s) 902
BpmI CTGGAG 1 cut(s) 332
BpuMI CCSGG 3 cut(s) 152, 290, 936
BsaAI YACGTR 1 cut(s) 211
BsaBI GATNNNNATC 2 cut(s) 803, 930
BsaHI GRCGYC 6 cut(s) 228, 365, 434, 569, 717, 993
BsaJI CCNNGG 3 cut(s) 241, 414, 977
BsaWI WCCGGW 2 cut(s) 749, 940
BsaXI ACNNNNNCTCC 8 cut(s) 299, 329, 515, 545, 617, 647, 736, 766
Bsc4I CCNNNNNNNGG 2 cut(s) 420, 654
Bse118I RCCGGY 1 cut(s) 713
Bse1I ACTGG 2 cut(s) 897, 993
Bse3DI GCAATG 1 cut(s) 222
Bse8I GATNNNNATC 2 cut(s) 803, 930
BseDI CCNNGG 3 cut(s) 241, 414, 977
BseGI GGATG 8 cut(s) 24, 202, 492, 540, 786, 804, 931, 1021
BseJI GATNNNNATC 2 cut(s) 803, 930
BseLI CCNNNNNNNGG 2 cut(s) 420, 654
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 1 cut(s) 91
BseNI ACTGG 2 cut(s) 897, 993
BseRI GAGGAG 6 cut(s) 271, 307, 310, 313, 335, 622
BseSI GKGCMC 3 cut(s) 587, 1010, 1031
BseX3I CGGCCG 1 cut(s) 411
BseXI GCAGC 2 cut(s) 134, 939
BseYI CCCAGC 2 cut(s) 143, 580
Bsh1236I CGCG 2 cut(s) 605, 1058
Bsh1285I CGRYCG 2 cut(s) 414, 1004
BshFI GGCC 6 cut(s) 185, 254, 413, 585, 698, 1029
BsiEI CGRYCG 2 cut(s) 414, 1004
BsiHKAI GWGCWC 3 cut(s) 174, 194, 816
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 8 cut(s) 152, 290, 608, 714, 750, 849, 935, 941
BslFI GGGAC 1 cut(s) 805
BslI CCNNNNNNNGG 2 cut(s) 420, 654
BsmAI GTCTC 2 cut(s) 319, 979
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 805
BsnI GGCC 6 cut(s) 185, 254, 413, 585, 698, 1029
BsoBI CYCGRG 1 cut(s) 815
Bsp120I GGGCCC 2 cut(s) 583, 1027
Bsp1286I GDGCHC 7 cut(s) 174, 194, 426, 587, 816, 1010, 1031
Bsp143I GATC 5 cut(s) 438, 746, 768, 804, 1036
BspANI GGCC 6 cut(s) 185, 254, 413, 585, 698, 1029
BspCNI CTCAG 1 cut(s) 90
BspFNI CGCG 2 cut(s) 605, 1058
BspLI GGNNCC 8 cut(s) 585, 586, 820, 821, 881, 939, 1028, 1029
BspPI GGATC 4 cut(s) 433, 741, 776, 812
BsrBI CCGCTC 3 cut(s) 444, 463, 963
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 713
BsrI ACTGG 2 cut(s) 897, 993
BssAI RCCGGY 1 cut(s) 713
BssECI CCNNGG 3 cut(s) 241, 414, 977
BssMI GATC 5 cut(s) 438, 746, 768, 804, 1036
BssNI GRCGYC 6 cut(s) 228, 365, 434, 569, 717, 993
BssSI CACGAG 1 cut(s) 167
Bst2BI CACGAG 1 cut(s) 167
Bst4CI ACNGT 1 cut(s) 1005
BstACI GRCGYC 6 cut(s) 228, 365, 434, 569, 717, 993
BstAPI GCANNNNNTGC 1 cut(s) 699
BstBAI YACGTR 1 cut(s) 211
BstC8I GCNNGC 6 cut(s) 90, 461, 475, 567, 700, 950
BstDEI CTNAG 1 cut(s) 77
BstDSI CCRYGG 3 cut(s) 241, 414, 977
BstF5I GGATG 8 cut(s) 24, 202, 492, 540, 786, 804, 931, 1021
BstFNI CGCG 2 cut(s) 605, 1058
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 3 cut(s) 88, 607, 1060
BstKTI GATC 5 cut(s) 441, 749, 771, 807, 1039
BstMAI GTCTC 2 cut(s) 319, 979
BstMBI GATC 5 cut(s) 438, 746, 768, 804, 1036
BstMCI CGRYCG 2 cut(s) 414, 1004
BstMWI GCNNNNNNNGC 2 cut(s) 699, 793
BstNSI RCATGY 1 cut(s) 507
BstSCI CCNGG 3 cut(s) 150, 288, 934
BstSFI CTRYAG 1 cut(s) 788
BstSLI GKGCMC 3 cut(s) 587, 1010, 1031
BstUI CGCG 2 cut(s) 605, 1058
BstV1I GCAGC 2 cut(s) 134, 939
BstX2I RGATCY 2 cut(s) 746, 804
BstXI CCANNNNNNTGG 1 cut(s) 580
BstYI RGATCY 2 cut(s) 746, 804
BstZI CGGCCG 1 cut(s) 411
BsuRI GGCC 6 cut(s) 185, 254, 413, 585, 698, 1029
BtgI CCRYGG 3 cut(s) 241, 414, 977
BtgZI GCGATG 1 cut(s) 841
BtsCI GGATG 8 cut(s) 24, 202, 492, 540, 786, 804, 931, 1021
Cac8I GCNNGC 6 cut(s) 90, 461, 475, 567, 700, 950
CfoI GCGC 3 cut(s) 88, 607, 1060
Cfr10I RCCGGY 1 cut(s) 713
Cfr13I GGNCC 9 cut(s) 154, 205, 583, 584, 657, 819, 938, 1027, 1028
CseI GACGC 4 cut(s) 236, 558, 706, 1001
Csp6I GTAC 2 cut(s) 613, 757
CviAII CATG 3 cut(s) 187, 504, 721
CviQI GTAC 2 cut(s) 613, 757
DdeI CTNAG 1 cut(s) 77
DpnI GATC 5 cut(s) 440, 748, 770, 806, 1038
DpnII GATC 5 cut(s) 438, 746, 768, 804, 1036
EaeI YGGCCR 3 cut(s) 183, 252, 411
EagI CGGCCG 1 cut(s) 411
EciI GGCGGA 3 cut(s) 436, 500, 912
Ecl136II GAGCTC 1 cut(s) 814
EclXI CGGCCG 1 cut(s) 411
Eco147I AGGCCT 1 cut(s) 698
Eco24I GRGCYC 4 cut(s) 426, 587, 816, 1031
Eco47I GGWCC 5 cut(s) 154, 205, 657, 819, 938
Eco47III AGCGCT 1 cut(s) 87
Eco52I CGGCCG 1 cut(s) 411
Eco53kI GAGCTC 1 cut(s) 814
Eco72I CACGTG 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 815
EcoICRI GAGCTC 1 cut(s) 814
EcoO109I RGGNCCY 3 cut(s) 584, 819, 1027
EcoT38I GRGCYC 4 cut(s) 426, 587, 816, 1031
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 3 cut(s) 190, 507, 724
FaiI YATR 6 cut(s) 188, 428, 505, 722, 1033, 1035
FaqI GGGAC 1 cut(s) 805
FatI CATG 3 cut(s) 186, 503, 720
FauI CCCGC 5 cut(s) 412, 468, 585, 834, 956
FauNDI CATATG 1 cut(s) 1033
FblI GTMKAC 4 cut(s) 393, 522, 549, 867
Fnu4HI GCNGC 6 cut(s) 148, 231, 255, 603, 861, 953
FokI GGATG 8 cut(s) 11, 209, 479, 527, 793, 811, 918, 1028
FriOI GRGCYC 4 cut(s) 426, 587, 816, 1031
Fsp4HI GCNGC 6 cut(s) 148, 231, 255, 603, 861, 953
GlaI GCGC 3 cut(s) 87, 606, 1059
GluI GCNGC 6 cut(s) 148, 231, 255, 603, 861, 953
GsaI CCCAGC 2 cut(s) 147, 584
GsuI CTGGAG 1 cut(s) 332
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 6 cut(s) 185, 254, 413, 585, 698, 1029
HapII CCGG 8 cut(s) 152, 290, 608, 714, 750, 849, 935, 941
HgaI GACGC 4 cut(s) 236, 558, 706, 1001
HhaI GCGC 3 cut(s) 88, 607, 1060
Hin1I GRCGYC 6 cut(s) 228, 365, 434, 569, 717, 993
Hin1II CATG 3 cut(s) 190, 507, 724
Hin6I GCGC 3 cut(s) 86, 605, 1058
HinP1I GCGC 3 cut(s) 86, 605, 1058
HincII GTYRAC 2 cut(s) 550, 868
HindII GTYRAC 2 cut(s) 550, 868
HinfI GANTC 7 cut(s) 75, 111, 390, 407, 516, 530, 1064
HpaII CCGG 8 cut(s) 152, 290, 608, 714, 750, 849, 935, 941
HphI GGTGA 3 cut(s) 670, 955, 994
Hpy166II GTNNAC 7 cut(s) 208, 394, 523, 550, 573, 759, 868
Hpy188I TCNGA 4 cut(s) 70, 196, 389, 535
Hpy188III TCNNGA 3 cut(s) 685, 923, 1068
Hpy8I GTNNAC 7 cut(s) 208, 394, 523, 550, 573, 759, 868
Hpy99I CGWCG 7 cut(s) 230, 367, 407, 436, 439, 551, 554
HpyAV CCTTC 5 cut(s) 38, 409, 691, 961, 1063
HpyCH4III ACNGT 1 cut(s) 1005
HpyCH4IV ACGT 4 cut(s) 210, 365, 434, 650
HpyCH4V TGCA 3 cut(s) 618, 931, 955
HpyF10VI GCNNNNNNNGC 2 cut(s) 699, 793
HpyF3I CTNAG 1 cut(s) 77
HpySE526I ACGT 4 cut(s) 210, 365, 434, 650
Hsp92I GRCGYC 6 cut(s) 228, 365, 434, 569, 717, 993
Hsp92II CATG 3 cut(s) 190, 507, 724
HspAI GCGC 3 cut(s) 86, 605, 1058
KflI GGGWCCC 1 cut(s) 819
Kzo9I GATC 5 cut(s) 438, 746, 768, 804, 1036
LmnI GCTCC 3 cut(s) 449, 784, 960
Lsp1109I GCAGC 2 cut(s) 134, 939
LweI GCATC 3 cut(s) 789, 940, 1006
MaeII ACGT 4 cut(s) 210, 365, 434, 650
MaeIII GTNAC 1 cut(s) 1041
MalI GATC 5 cut(s) 440, 748, 770, 806, 1038
MbiI CCGCTC 3 cut(s) 444, 463, 963
MboI GATC 5 cut(s) 438, 746, 768, 804, 1036
MboII GAAGA 1 cut(s) 295
MflI RGATCY 2 cut(s) 746, 804
MhlI GDGCHC 7 cut(s) 174, 194, 426, 587, 816, 1010, 1031
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 3 cut(s) 128, 354, 383
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 5 cut(s) 69, 399, 401, 524, 525
MmeI TCCRAC 4 cut(s) 298, 357, 558, 684
Mox20I TGGCCA 1 cut(s) 185
MscI TGGCCA 1 cut(s) 185
MslI CAYNNNNRTG 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 185
MspA1I CMGCKG 3 cut(s) 147, 179, 580
MspI CCGG 8 cut(s) 152, 290, 608, 714, 750, 849, 935, 941
MspR9I CCNGG 3 cut(s) 152, 290, 936
MvnI CGCG 2 cut(s) 605, 1058
MwoI GCNNNNNNNGC 2 cut(s) 699, 793
NciI CCSGG 3 cut(s) 152, 290, 936
NdeI CATATG 1 cut(s) 1033
NdeII GATC 5 cut(s) 438, 746, 768, 804, 1036
NlaIII CATG 3 cut(s) 190, 507, 724
NlaIV GGNNCC 8 cut(s) 585, 586, 820, 821, 881, 939, 1028, 1029
NmeAIII GCCGAG 2 cut(s) 389, 539
NspI RCATGY 1 cut(s) 507
OliI CACNNNNGTG 1 cut(s) 179
PceI AGGCCT 1 cut(s) 698
PciI ACATGT 1 cut(s) 503
PcsI WCGNNNNNNNCGW 3 cut(s) 411, 525, 864
PfeI GAWTC 1 cut(s) 111
PflMI CCANNNNNTGG 1 cut(s) 654
PkrI GCNGC 6 cut(s) 149, 232, 256, 604, 862, 954
PleI GAGTC 5 cut(s) 69, 398, 401, 524, 524
PmaCI CACGTG 1 cut(s) 211
PmlI CACGTG 1 cut(s) 211
PpsI GAGTC 5 cut(s) 69, 398, 401, 524, 524
Ppu21I YACGTR 1 cut(s) 211
PpuMI RGGWCCY 1 cut(s) 819
PscI ACATGT 1 cut(s) 503
Psp124BI GAGCTC 1 cut(s) 816
Psp5II RGGWCCY 1 cut(s) 819
PspCI CACGTG 1 cut(s) 211
PspFI CCCAGC 2 cut(s) 143, 580
PspN4I GGNNCC 8 cut(s) 585, 586, 820, 821, 881, 939, 1028, 1029
PspOMI GGGCCC 2 cut(s) 583, 1027
PspPI GGNCC 9 cut(s) 154, 205, 583, 584, 657, 819, 938, 1027, 1028
PspPPI RGGWCCY 1 cut(s) 819
PsuI RGATCY 2 cut(s) 746, 804
PvuII CAGCTG 1 cut(s) 147
RsaI GTAC 2 cut(s) 614, 758
RsaNI GTAC 2 cut(s) 613, 757
RseI CAYNNNNRTG 1 cut(s) 179
SacI GAGCTC 1 cut(s) 816
SalI GTCGAC 2 cut(s) 548, 866
SatI GCNGC 6 cut(s) 148, 231, 255, 603, 861, 953
Sau3AI GATC 5 cut(s) 438, 746, 768, 804, 1036
Sau96I GGNCC 9 cut(s) 154, 205, 583, 584, 657, 819, 938, 1027, 1028
ScaI AGTACT 1 cut(s) 614
SchI GAGTC 5 cut(s) 69, 399, 401, 524, 525
ScrFI CCNGG 3 cut(s) 152, 290, 936
SduI GDGCHC 7 cut(s) 174, 194, 426, 587, 816, 1010, 1031
SfaNI GCATC 3 cut(s) 789, 940, 1006
SfcI CTRYAG 1 cut(s) 788
SgrAI CRCCGGYG 1 cut(s) 713
SgrDI CGTCGACG 1 cut(s) 548
SinI GGWCC 5 cut(s) 154, 205, 657, 819, 938
SmiMI CAYNNNNRTG 1 cut(s) 179
Sse9I AATT 3 cut(s) 128, 354, 383
SseBI AGGCCT 1 cut(s) 698
SstI GAGCTC 1 cut(s) 816
StuI AGGCCT 1 cut(s) 698
StyD4I CCNGG 3 cut(s) 150, 288, 934
TaaI ACNGT 1 cut(s) 1005
TaiI ACGT 4 cut(s) 213, 368, 437, 653
TasI AATT 3 cut(s) 128, 354, 383
TatI WGTACW 1 cut(s) 612
TauI GCSGC 4 cut(s) 233, 257, 605, 863
TfiI GAWTC 1 cut(s) 111
TseI GCWGC 2 cut(s) 147, 952
TspDTI ATGAA 1 cut(s) 13
TspGWI ACGGA 5 cut(s) 230, 256, 776, 781, 903
Van91I CCANNNNNTGG 1 cut(s) 654
VpaK11BI GGWCC 5 cut(s) 154, 205, 657, 819, 938
XapI RAATTY 2 cut(s) 128, 383
XceI RCATGY 1 cut(s) 507
XmiI GTMKAC 4 cut(s) 393, 522, 549, 867
ZraI GACGTC 2 cut(s) 366, 435
ZrmI AGTACT 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.