MD02G1168700.v1.1

Enoyl-(Acyl carrier protein) reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014176
Physical Location & Seq
Reverse (-)
14461680 .. 14463756
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1168700.v1.1.491

Sequence Viewer

Length: 792 bp
ATGAAGAGCATGGCAAGCTCAGCTCCTCAGCGAGGTATAGCCGCCATCGTGGGCGTCGGGCCGAAGCTGGGCCGCTCCATAGCCCGCAAGTTCGTCCACGAAGGCTATACCGTCGCTATTCTCGCCCGCGATCTCGGGAGGCTCTCGAAATTCGCAGACGAAATCGCGAGGGAAGAGAAGGCCCAGGTTTTTGCCATTAGGATAGACTGCTCCGAGTCTAGAAGCGTGAGGGAGGCATTTGAAGGGGTTTTGTCTTTGGGTTTTGTGGAGGTGCTGGTCTACAATGCGTACCAGCAGCCGCCCAATTGGCACCCCACCAACTTCACTGACATCCGCCTCGACTCGTTTGAGAAGTCCCTTGCCGTTTCTTCCGTCGGAGCCTTTCACTGCGCTCAACAGGTGCTTCCGGGAATGGTGGAAAGAGGAAGGGGAACAATTTTGTTTACGGGGTGTTCCGGTTCACTCAAGGGCATTGCTGGCCGTTCTGAACTCTGTTGCGGAAAATTCGCATTGAGAGCGTTATCACAATGTTTGTCAATGGAGTTTCAACCGCAGGGGATACACATAGCTCACGTGATCATTGACGGTGTGATTGGCCCACCTCGAGAGGGCCAATCATCATCGAGTGCTTCACAGAGGACGACGACGTTAGGTACAACGCGGTTAGGGGTTGACGTGATAATGGATCCCGACGCGGTGGCTCAAACCTACTGGCAGTTGCACGTCCAAGACCGGAGTGCTTGGACCCAAGAAATCGACCTCCGTCCATCTAACCCCCCCAAATTCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

264

Amino Acids

28.61

Weight (kDa)

8.49

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 193 2.2e-28 short chain dehydrogenase
adh_short_C2 PF13561 23 - 246 9.2e-22 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014176)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50560
fragaria_vesca FvH4_1g15680
malus_domestica MD02G1168700.v1.1
prunus_persica Prupe.7G136500_v2.0.a1 Prupe.7G136500_v2.0.a1
pyrus_communis pycom02g13560 pycom15g24410
rosa_chinensis RchiOBHm_Chr2g0104991
rosa_laevigata RLG00000017387
rosa_multiflora Rmu_sc0000641.1_g000031
rosa_roxburghii Rroxscaffold_2G00138600
rosa_rugosa Rorug02G0124800
rosa_samantha Rh2AG175200 Rh2BG183100 Rh2CG179800 Rh2DG181600
rosa_wichuraiana Rw2G013770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 309
AccBSI CCGCTC 1 cut(s) 75
AccI GTMKAC 1 cut(s) 279
AccII CGCG 4 cut(s) 129, 167, 661, 695
AclWI GGATC 2 cut(s) 680, 693
AcoI YGGCCR 1 cut(s) 478
AcsI RAATTY 3 cut(s) 149, 503, 782
AcvI CACGTG 1 cut(s) 574
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 290, 655
AfiI CCNNNNNNNGG 3 cut(s) 32, 68, 608
AgsI TTSAA 2 cut(s) 242, 548
AjiI CACGTC 2 cut(s) 676, 724
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 4 cut(s) 18, 23, 67, 569
AluI AGCT 4 cut(s) 18, 23, 67, 569
AlwI GGATC 2 cut(s) 680, 693
Ama87I CYCGRG 2 cut(s) 134, 603
AoxI GGCC 6 cut(s) 59, 70, 180, 478, 595, 610
ApeKI GCWGC 1 cut(s) 295
ApoI RAATTY 3 cut(s) 149, 503, 782
AspLEI GCGC 1 cut(s) 392
AspS9I GGNCC 6 cut(s) 59, 70, 181, 596, 610, 744
AsuC2I CCSGG 1 cut(s) 408
AvaI CYCGRG 2 cut(s) 134, 603
AvaII GGWCC 1 cut(s) 744
BamHI GGATCC 1 cut(s) 685
BanI GGYRCC 1 cut(s) 309
BbrPI CACGTG 1 cut(s) 574
BbvCI CCTCAGC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 307
BccI CCATC 2 cut(s) 53, 775
BceAI ACGGC 2 cut(s) 347, 465
BciT130I CCWGG 1 cut(s) 185
BciVI GTATCC 1 cut(s) 552
BclI TGATCA 1 cut(s) 576
BcnI CCSGG 1 cut(s) 408
BfaI CTAG 1 cut(s) 219
BfuI GTATCC 1 cut(s) 552
BglI GCCNNNNNGGC 1 cut(s) 307
BisI GCNGC 4 cut(s) 42, 73, 296, 299
BlpI GCTNAGC 1 cut(s) 19
BlsI GCNGC 4 cut(s) 43, 74, 297, 300
Bme1390I CCNGG 2 cut(s) 185, 408
Bme18I GGWCC 1 cut(s) 744
BmeT110I CYCGRG 2 cut(s) 134, 603
BmgBI CACGTC 2 cut(s) 676, 724
BmgT120I GGNCC 6 cut(s) 59, 70, 181, 596, 610, 744
BmiI GGNNCC 4 cut(s) 311, 379, 687, 746
BmrFI CCNGG 2 cut(s) 185, 408
BoxI GACNNNNGTC 1 cut(s) 762
Bpu10I CCTNAGC 1 cut(s) 27
Bpu1102I GCTNAGC 1 cut(s) 19
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 574
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 183
BsaWI WCCGGW 2 cut(s) 455, 732
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 68, 608
Bse1I ACTGG 1 cut(s) 716
Bse3DI GCAATG 1 cut(s) 471
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 1 cut(s) 183
BseGI GGATG 1 cut(s) 330
BseLI CCNNNNNNNGG 3 cut(s) 32, 68, 608
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 2 cut(s) 33, 41
BseNI ACTGG 1 cut(s) 716
BseRI GAGGAG 1 cut(s) 15
BseXI GCAGC 1 cut(s) 307
BseYI CCCAGC 1 cut(s) 67
Bsh1236I CGCG 4 cut(s) 129, 167, 661, 695
BshFI GGCC 6 cut(s) 61, 72, 182, 480, 597, 612
BshNI GGYRCC 1 cut(s) 309
BsiHKCI CYCGRG 2 cut(s) 134, 603
BsiSI CCGG 3 cut(s) 407, 456, 733
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 3 cut(s) 32, 68, 608
BsmFI GGGAC 1 cut(s) 340
BsnI GGCC 6 cut(s) 61, 72, 182, 480, 597, 612
BsoBI CYCGRG 2 cut(s) 134, 603
Bsp143I GATC 3 cut(s) 130, 576, 685
Bsp1720I GCTNAGC 1 cut(s) 19
Bsp68I TCGCGA 1 cut(s) 167
BspANI GGCC 6 cut(s) 61, 72, 182, 480, 597, 612
BspCNI CTCAG 2 cut(s) 32, 40
BspFNI CGCG 4 cut(s) 129, 167, 661, 695
BspLI GGNNCC 4 cut(s) 311, 379, 687, 746
BspPI GGATC 2 cut(s) 680, 693
BspT107I GGYRCC 1 cut(s) 309
BsrBI CCGCTC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 471
BsrI ACTGG 1 cut(s) 716
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 3 cut(s) 130, 576, 685
BssNI GRCGYC 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 2 cut(s) 112, 587
Bst6I CTCTTC 1 cut(s) 168
BstACI GRCGYC 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 574
BstC8I GCNNGC 4 cut(s) 16, 85, 127, 478
BstDEI CTNAG 2 cut(s) 19, 27
BstENI CCTNNNNNAGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 330
BstFNI CGCG 4 cut(s) 129, 167, 661, 695
BstHHI GCGC 1 cut(s) 392
BstKTI GATC 3 cut(s) 133, 579, 688
BstMBI GATC 3 cut(s) 130, 576, 685
BstMWI GCNNNNNNNGC 6 cut(s) 15, 20, 122, 307, 477, 515
BstNI CCWGG 1 cut(s) 185
BstPAI GACNNNNGTC 1 cut(s) 762
BstSCI CCNGG 2 cut(s) 183, 406
BstUI CGCG 4 cut(s) 129, 167, 661, 695
BstV1I GCAGC 1 cut(s) 307
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
BsuI GTATCC 1 cut(s) 552
BsuRI GGCC 6 cut(s) 61, 72, 182, 480, 597, 612
BtrI CACGTC 2 cut(s) 676, 724
BtsCI GGATG 1 cut(s) 330
BtsI GCAGTG 1 cut(s) 385
BtsIMutI CAGTG 2 cut(s) 324, 385
BtuMI TCGCGA 1 cut(s) 167
Cac8I GCNNGC 4 cut(s) 16, 85, 127, 478
CfoI GCGC 1 cut(s) 392
Cfr13I GGNCC 6 cut(s) 59, 70, 181, 596, 610, 744
CseI GACGC 2 cut(s) 43, 701
Csp6I GTAC 2 cut(s) 289, 654
CviAII CATG 1 cut(s) 10
CviQI GTAC 2 cut(s) 289, 654
DdeI CTNAG 2 cut(s) 19, 27
DpnI GATC 3 cut(s) 132, 578, 687
DpnII GATC 3 cut(s) 130, 576, 685
EaeI YGGCCR 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
EciI GGCGGA 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 744
Eco72I CACGTG 1 cut(s) 574
Eco88I CYCGRG 2 cut(s) 134, 603
EcoNI CCTNNNNNAGG 1 cut(s) 30
EcoRII CCWGG 1 cut(s) 183
FaeI CATG 1 cut(s) 13
FaiI YATR 5 cut(s) 11, 38, 80, 108, 566
FaqI GGGAC 1 cut(s) 340
FatI CATG 1 cut(s) 9
FauI CCCGC 2 cut(s) 92, 134
FbaI TGATCA 1 cut(s) 576
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 4 cut(s) 42, 73, 296, 299
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 4 cut(s) 42, 73, 296, 299
FspBI CTAG 1 cut(s) 219
GlaI GCGC 1 cut(s) 391
GluI GCNGC 4 cut(s) 42, 73, 296, 299
GsaI CCCAGC 1 cut(s) 71
HaeIII GGCC 6 cut(s) 61, 72, 182, 480, 597, 612
HapII CCGG 3 cut(s) 407, 456, 733
HgaI GACGC 2 cut(s) 43, 701
HhaI GCGC 1 cut(s) 392
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 1 cut(s) 13
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HinfI GANTC 2 cut(s) 215, 341
HpaII CCGG 3 cut(s) 407, 456, 733
Hpy166II GTNNAC 5 cut(s) 97, 280, 444, 461, 673
Hpy188I TCNGA 3 cut(s) 214, 377, 487
Hpy188III TCNNGA 6 cut(s) 136, 145, 166, 219, 605, 689
Hpy8I GTNNAC 5 cut(s) 97, 280, 444, 461, 673
Hpy99I CGWCG 6 cut(s) 59, 116, 377, 646, 649, 695
HpyAV CCTTC 4 cut(s) 95, 172, 236, 420
HpyCH4III ACNGT 2 cut(s) 112, 587
HpyCH4IV ACGT 4 cut(s) 573, 647, 675, 723
HpyCH4V TGCA 1 cut(s) 721
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 20, 122, 307, 477, 515
HpyF3I CTNAG 2 cut(s) 19, 27
HpySE526I ACGT 4 cut(s) 573, 647, 675, 723
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 1 cut(s) 13
HspAI GCGC 1 cut(s) 390
Ksp22I TGATCA 1 cut(s) 576
Kzo9I GATC 3 cut(s) 130, 576, 685
LmnI GCTCC 4 cut(s) 28, 80, 215, 377
Lsp1109I GCAGC 1 cut(s) 307
MaeI CTAG 1 cut(s) 219
MaeII ACGT 4 cut(s) 573, 647, 675, 723
MalI GATC 3 cut(s) 132, 578, 687
MbiI CCGCTC 1 cut(s) 75
MboI GATC 3 cut(s) 130, 576, 685
MboII GAAGA 3 cut(s) 16, 185, 360
MfeI CAATTG 1 cut(s) 304
MflI RGATCY 1 cut(s) 685
MluCI AATT 5 cut(s) 149, 304, 435, 503, 782
MlyI GAGTC 2 cut(s) 224, 335
MmeI TCCRAC 1 cut(s) 355
MspI CCGG 3 cut(s) 407, 456, 733
MspR9I CCNGG 2 cut(s) 185, 408
MunI CAATTG 1 cut(s) 304
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 4 cut(s) 129, 167, 661, 695
MwoI GCNNNNNNNGC 6 cut(s) 15, 20, 122, 307, 477, 515
NciI CCSGG 1 cut(s) 408
NdeII GATC 3 cut(s) 130, 576, 685
NlaIII CATG 1 cut(s) 13
NlaIV GGNNCC 4 cut(s) 311, 379, 687, 746
NruI TCGCGA 1 cut(s) 167
PaeR7I CTCGAG 1 cut(s) 603
PfoI TCCNGGA 1 cut(s) 406
PkrI GCNGC 4 cut(s) 43, 74, 297, 300
PleI GAGTC 2 cut(s) 223, 335
PmaCI CACGTG 1 cut(s) 574
PmlI CACGTG 1 cut(s) 574
PpsI GAGTC 2 cut(s) 223, 335
Ppu21I YACGTR 1 cut(s) 574
PshAI GACNNNNGTC 1 cut(s) 762
Psp6I CCWGG 1 cut(s) 183
PspCI CACGTG 1 cut(s) 574
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 4 cut(s) 311, 379, 687, 746
PspPI GGNCC 6 cut(s) 59, 70, 181, 596, 610, 744
PsuI RGATCY 1 cut(s) 685
RruI TCGCGA 1 cut(s) 167
RsaI GTAC 2 cut(s) 290, 655
RsaNI GTAC 2 cut(s) 289, 654
SatI GCNGC 4 cut(s) 42, 73, 296, 299
Sau3AI GATC 3 cut(s) 130, 576, 685
Sau96I GGNCC 6 cut(s) 59, 70, 181, 596, 610, 744
SchI GAGTC 2 cut(s) 224, 335
ScrFI CCNGG 2 cut(s) 185, 408
Sfr274I CTCGAG 1 cut(s) 603
SinI GGWCC 1 cut(s) 744
SlaI CTCGAG 1 cut(s) 603
SmlI CTYRAG 2 cut(s) 464, 603
SmoI CTYRAG 2 cut(s) 464, 603
Sse9I AATT 5 cut(s) 149, 304, 435, 503, 782
SspMI CTAG 1 cut(s) 219
StyD4I CCNGG 2 cut(s) 183, 406
TaaI ACNGT 2 cut(s) 112, 587
TaiI ACGT 4 cut(s) 576, 650, 678, 726
TaqI TCGA 5 cut(s) 146, 339, 604, 623, 756
TasI AATT 5 cut(s) 149, 304, 435, 503, 782
TauI GCSGC 3 cut(s) 44, 75, 301
TscAI CASTG 2 cut(s) 331, 392
TseI GCWGC 1 cut(s) 295
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 361, 752
TspRI CASTG 2 cut(s) 331, 392
VpaK11BI GGWCC 1 cut(s) 744
XagI CCTNNNNNAGG 1 cut(s) 30
XapI RAATTY 3 cut(s) 149, 503, 782
XbaI TCTAGA 1 cut(s) 218
XhoI CTCGAG 1 cut(s) 603
XmiI GTMKAC 1 cut(s) 279
XspI CTAG 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.