RLG00000017387

Enoyl-(Acyl carrier protein) reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014176
Physical Location & Seq
Reverse (-)
15992073 .. 15993110
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017387

Sequence Viewer

Length: 774 bp
ATGATGCGCAACATGGCAAGCTCTGGTTCCAGTCGGGGCATCGCGGCCATTGTGGGCGTTGGACCGAATCTAGGCCGGTCCATCGCTCGGAAGTTCGCCCATGAAGGCTACACGGTGGCCATCCTTGCCCGTGATCTTGACAGACTGTCGAAATTTGCCGATGAGATTGCAAGGGAGGAGAAATCTCAAGTGTTTGCCATAAGAATCGACTGCTCCGAGTCTCGGAGCGTGAGGGAGGCCTTTGAGGGTGTTCTTTCCCTTGGTTTTGTGGAGGTGCTGGTCTACAATGCTTACCAGCCGGTTTCTTGGAAACCCACAAAGTTCACCGACATTCGGATCGACTCCTTGGAGAAGTCCCTCGCCGTCTCTTCTGTCGGAGCCTTTCACTGTGCTCAACAGGTTCTTCCTGGTATGGTTGAAAGGGGAAGGGGTACGATTTTGTTCACCGGATGTTCAGCTTCACTTAAAGGCATTGCTGGCTACTCTGAACTATGCTGTGGGAAATTTGCACTGAGAGCTCTATCACAATGTTTGTCCATGGAGTTCCAACCTTACGGAGTACATATTGCGCATGTTATTGTTGACGGGGTCATGGGACCGCCTAGGGGTGGCTCATCATCGAGTTCGCAGAGAATTGCCGGAGTAGGAGTCAGCGATGGATCAATGGACCCGGACGCGGTGGCTCAAACCTATTGGCACTTGCACATTCAAGACCGGAGTGCTTGGACCCAAGAAATCGACCTCCGTCCTTCAATCCCTCCCAGATTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

258

Amino Acids

27.82

Weight (kDa)

8.17

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 14 - 193 7.4e-30 short chain dehydrogenase
adh_short_C2 PF13561 23 - 193 9.6e-21 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014176)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50560
fragaria_vesca FvH4_1g15680
malus_domestica MD02G1168700.v1.1
prunus_persica Prupe.7G136500_v2.0.a1 Prupe.7G136500_v2.0.a1
pyrus_communis pycom02g13560 pycom15g24410
rosa_chinensis RchiOBHm_Chr2g0104991
rosa_laevigata RLG00000017387
rosa_multiflora Rmu_sc0000641.1_g000031
rosa_roxburghii Rroxscaffold_2G00138600
rosa_rugosa Rorug02G0124800
rosa_samantha Rh2AG175200 Rh2BG183100 Rh2CG179800 Rh2DG181600
rosa_wichuraiana Rw2G013770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 8, 570
AccI GTMKAC 1 cut(s) 282
AccII CGCG 2 cut(s) 44, 677
AciI CCGC 3 cut(s) 44, 599, 677
AclWI GGATC 2 cut(s) 344, 667
AcoI YGGCCR 2 cut(s) 45, 117
AcsI RAATTY 2 cut(s) 152, 503
AfaI GTAC 2 cut(s) 433, 561
AfiI CCNNNNNNNGG 7 cut(s) 71, 87, 222, 333, 605, 608, 676
AgsI TTSAA 3 cut(s) 419, 710, 753
AhdI GACNNNNNGTC 1 cut(s) 145
AjnI CCWGG 1 cut(s) 406
AluBI AGCT 3 cut(s) 21, 458, 518
AluI AGCT 3 cut(s) 21, 458, 518
Alw21I GWGCWC 2 cut(s) 394, 520
Alw26I GTCTC 2 cut(s) 225, 370
AlwI GGATC 2 cut(s) 344, 667
AoxI GGCC 4 cut(s) 45, 73, 117, 237
ApoI RAATTY 2 cut(s) 152, 503
AspA2I CCTAGG 1 cut(s) 602
AspLEI GCGC 2 cut(s) 9, 571
AspS9I GGNCC 5 cut(s) 62, 78, 596, 667, 726
AsuC2I CCSGG 1 cut(s) 671
AsuHPI GGTGA 2 cut(s) 316, 436
AvaII GGWCC 5 cut(s) 62, 78, 596, 667, 726
AvrII CCTAGG 1 cut(s) 602
BalI TGGCCA 1 cut(s) 119
BanII GRGCYC 1 cut(s) 520
Bbv12I GWGCWC 2 cut(s) 394, 520
BccI CCATC 3 cut(s) 89, 128, 650
BceAI ACGGC 1 cut(s) 347
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BciT130I CCWGG 1 cut(s) 408
BcnI CCSGG 1 cut(s) 671
BcoDI GTCTC 2 cut(s) 225, 370
BfaI CTAG 3 cut(s) 71, 603, 772
BisI GCNGC 1 cut(s) 45
BlnI CCTAGG 1 cut(s) 602
BlsI GCNGC 1 cut(s) 46
Bme1390I CCNGG 2 cut(s) 408, 671
Bme18I GGWCC 5 cut(s) 62, 78, 596, 667, 726
BmeRI GACNNNNNGTC 1 cut(s) 145
BmgT120I GGNCC 5 cut(s) 62, 78, 596, 667, 726
BmiI GGNNCC 5 cut(s) 28, 379, 597, 669, 728
BmrFI CCNGG 2 cut(s) 408, 671
BmsI GCATC 1 cut(s) 48
BoxI GACNNNNGTC 1 cut(s) 744
BpuEI CTTGAG 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 671
BsaJI CCNNGG 4 cut(s) 259, 345, 537, 602
BsaWI WCCGGW 2 cut(s) 446, 714
BsaXI ACNNNNNCTCC 2 cut(s) 633, 663
Bsc4I CCNNNNNNNGG 7 cut(s) 71, 87, 222, 333, 605, 608, 676
Bse118I RCCGGY 2 cut(s) 75, 298
Bse1I ACTGG 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 471
BseBI CCWGG 1 cut(s) 408
BseDI CCNNGG 4 cut(s) 259, 345, 537, 602
BseGI GGATG 2 cut(s) 120, 455
BseLI CCNNNNNNNGG 7 cut(s) 71, 87, 222, 333, 605, 608, 676
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 1 cut(s) 503
BseNI ACTGG 1 cut(s) 30
BseRI GAGGAG 1 cut(s) 191
Bsh1236I CGCG 2 cut(s) 44, 677
BshFI GGCC 4 cut(s) 47, 75, 119, 239
BsiHKAI GWGCWC 2 cut(s) 394, 520
BsiSI CCGG 6 cut(s) 76, 299, 447, 639, 671, 715
BslFI GGGAC 2 cut(s) 340, 609
BslI CCNNNNNNNGG 7 cut(s) 71, 87, 222, 333, 605, 608, 676
BsmAI GTCTC 2 cut(s) 225, 370
BsmBI CGTCTC 1 cut(s) 370
BsmFI GGGAC 2 cut(s) 340, 609
BsnI GGCC 4 cut(s) 47, 75, 119, 239
Bsp1286I GDGCHC 2 cut(s) 394, 520
Bsp143I GATC 3 cut(s) 133, 336, 659
Bsp19I CCATGG 1 cut(s) 537
BspACI CCGC 3 cut(s) 44, 599, 677
BspANI GGCC 4 cut(s) 47, 75, 119, 239
BspCNI CTCAG 1 cut(s) 504
BspFNI CGCG 2 cut(s) 44, 677
BspLI GGNNCC 5 cut(s) 28, 379, 597, 669, 728
BspPI GGATC 2 cut(s) 344, 667
BsrDI GCAATG 1 cut(s) 471
BsrFI RCCGGY 2 cut(s) 75, 298
BsrI ACTGG 1 cut(s) 30
BssAI RCCGGY 2 cut(s) 75, 298
BssECI CCNNGG 4 cut(s) 259, 345, 537, 602
BssMI GATC 3 cut(s) 133, 336, 659
BssT1I CCWWGG 4 cut(s) 259, 345, 537, 602
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 3 cut(s) 115, 147, 389
Bst6I CTCTTC 1 cut(s) 373
BstC8I GCNNGC 2 cut(s) 19, 478
BstDEI CTNAG 1 cut(s) 512
BstDSI CCRYGG 1 cut(s) 537
BstF5I GGATG 2 cut(s) 120, 455
BstFNI CGCG 2 cut(s) 44, 677
BstHHI GCGC 2 cut(s) 9, 571
BstKTI GATC 3 cut(s) 136, 339, 662
BstMAI GTCTC 2 cut(s) 225, 370
BstMBI GATC 3 cut(s) 133, 336, 659
BstMWI GCNNNNNNNGC 3 cut(s) 125, 477, 515
BstNI CCWGG 1 cut(s) 408
BstNSI RCATGY 1 cut(s) 575
BstPAI GACNNNNGTC 1 cut(s) 744
BstSCI CCNGG 2 cut(s) 406, 669
BstUI CGCG 2 cut(s) 44, 677
BsuRI GGCC 4 cut(s) 47, 75, 119, 239
BtgI CCRYGG 1 cut(s) 537
BtgZI GCGATG 3 cut(s) 25, 67, 669
BtsCI GGATG 2 cut(s) 120, 455
BtsIMutI CAGTG 2 cut(s) 385, 509
Cac8I GCNNGC 2 cut(s) 19, 478
CfoI GCGC 2 cut(s) 9, 571
Cfr10I RCCGGY 2 cut(s) 75, 298
Cfr13I GGNCC 5 cut(s) 62, 78, 596, 667, 726
CseI GACGC 1 cut(s) 683
Csp6I GTAC 2 cut(s) 432, 560
CviAII CATG 5 cut(s) 13, 101, 538, 572, 592
CviQI GTAC 2 cut(s) 432, 560
DdeI CTNAG 1 cut(s) 512
DpnI GATC 3 cut(s) 135, 338, 661
DpnII GATC 3 cut(s) 133, 336, 659
DriI GACNNNNNGTC 1 cut(s) 145
EaeI YGGCCR 2 cut(s) 45, 117
Eam1104I CTCTTC 1 cut(s) 373
Eam1105I GACNNNNNGTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 373
Ecl136II GAGCTC 1 cut(s) 518
Eco130I CCWWGG 4 cut(s) 259, 345, 537, 602
Eco147I AGGCCT 1 cut(s) 239
Eco24I GRGCYC 1 cut(s) 520
Eco47I GGWCC 5 cut(s) 62, 78, 596, 667, 726
Eco53kI GAGCTC 1 cut(s) 518
EcoICRI GAGCTC 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 406
EcoT14I CCWWGG 4 cut(s) 259, 345, 537, 602
EcoT38I GRGCYC 1 cut(s) 520
ErhI CCWWGG 4 cut(s) 259, 345, 537, 602
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 5 cut(s) 16, 104, 541, 575, 595
FaiI YATR 9 cut(s) 14, 102, 200, 413, 493, 539, 564, 573, 593
FaqI GGGAC 2 cut(s) 340, 609
FatI CATG 5 cut(s) 12, 100, 537, 571, 591
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 45
FokI GGATG 2 cut(s) 107, 462
FriOI GRGCYC 1 cut(s) 520
Fsp4HI GCNGC 1 cut(s) 45
FspBI CTAG 3 cut(s) 71, 603, 772
FspI TGCGCA 2 cut(s) 8, 570
GlaI GCGC 2 cut(s) 8, 570
GluI GCNGC 1 cut(s) 45
HaeIII GGCC 4 cut(s) 47, 75, 119, 239
HapII CCGG 6 cut(s) 76, 299, 447, 639, 671, 715
HgaI GACGC 1 cut(s) 683
HhaI GCGC 2 cut(s) 9, 571
Hin1II CATG 5 cut(s) 16, 104, 541, 575, 595
Hin6I GCGC 2 cut(s) 7, 569
HinP1I GCGC 2 cut(s) 7, 569
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HinfI GANTC 5 cut(s) 67, 204, 218, 341, 648
HpaII CCGG 6 cut(s) 76, 299, 447, 639, 671, 715
HphI GGTGA 2 cut(s) 316, 436
Hpy166II GTNNAC 4 cut(s) 283, 324, 444, 583
Hpy188I TCNGA 6 cut(s) 90, 217, 225, 336, 377, 487
Hpy188III TCNNGA 2 cut(s) 137, 710
Hpy8I GTNNAC 4 cut(s) 283, 324, 444, 583
HpyAV CCTTC 3 cut(s) 98, 420, 759
HpyCH4III ACNGT 3 cut(s) 115, 147, 389
HpyCH4V TGCA 3 cut(s) 170, 509, 703
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 477, 515
HpyF3I CTNAG 1 cut(s) 512
Hsp92II CATG 5 cut(s) 16, 104, 541, 575, 595
HspAI GCGC 2 cut(s) 7, 569
Kzo9I GATC 3 cut(s) 133, 336, 659
LmnI GCTCC 3 cut(s) 218, 225, 377
LweI GCATC 1 cut(s) 48
MaeI CTAG 3 cut(s) 71, 603, 772
MalI GATC 3 cut(s) 135, 338, 661
MboI GATC 3 cut(s) 133, 336, 659
MboII GAAGA 2 cut(s) 360, 395
MhlI GDGCHC 2 cut(s) 394, 520
MlsI TGGCCA 1 cut(s) 119
MluCI AATT 3 cut(s) 152, 503, 633
MluNI TGGCCA 1 cut(s) 119
MlyI GAGTC 3 cut(s) 227, 335, 657
MmeI TCCRAC 3 cut(s) 40, 355, 571
MnlI CCTC 8 cut(s) 169, 225, 229, 238, 265, 368, 752, 768
Mox20I TGGCCA 1 cut(s) 119
MscI TGGCCA 1 cut(s) 119
MseI TTAA 1 cut(s) 465
Msp20I TGGCCA 1 cut(s) 119
MspI CCGG 6 cut(s) 76, 299, 447, 639, 671, 715
MspR9I CCNGG 2 cut(s) 408, 671
MvaI CCWGG 1 cut(s) 408
MvnI CGCG 2 cut(s) 44, 677
MwoI GCNNNNNNNGC 3 cut(s) 125, 477, 515
NciI CCSGG 1 cut(s) 671
NcoI CCATGG 1 cut(s) 537
NdeII GATC 3 cut(s) 133, 336, 659
NlaIII CATG 5 cut(s) 16, 104, 541, 575, 595
NlaIV GGNNCC 5 cut(s) 28, 379, 597, 669, 728
NsbI TGCGCA 2 cut(s) 8, 570
NspI RCATGY 1 cut(s) 575
PceI AGGCCT 1 cut(s) 239
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PfeI GAWTC 2 cut(s) 67, 204
PflFI GACNNNGTC 1 cut(s) 587
PkrI GCNGC 1 cut(s) 46
PleI GAGTC 3 cut(s) 226, 335, 656
PpsI GAGTC 3 cut(s) 226, 335, 656
PshAI GACNNNNGTC 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 520
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 5 cut(s) 28, 379, 597, 669, 728
PspPI GGNCC 5 cut(s) 62, 78, 596, 667, 726
PsyI GACNNNGTC 1 cut(s) 587
RsaI GTAC 2 cut(s) 433, 561
RsaNI GTAC 2 cut(s) 432, 560
SacI GAGCTC 1 cut(s) 520
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 1 cut(s) 45
Sau3AI GATC 3 cut(s) 133, 336, 659
Sau96I GGNCC 5 cut(s) 62, 78, 596, 667, 726
SchI GAGTC 3 cut(s) 227, 335, 657
ScrFI CCNGG 2 cut(s) 408, 671
SduI GDGCHC 2 cut(s) 394, 520
SetI ASST 8 cut(s) 23, 276, 402, 460, 520, 553, 692, 744
SfaNI GCATC 1 cut(s) 48
SinI GGWCC 5 cut(s) 62, 78, 596, 667, 726
SmlI CTYRAG 1 cut(s) 186
SmoI CTYRAG 1 cut(s) 186
Sse9I AATT 3 cut(s) 152, 503, 633
SseBI AGGCCT 1 cut(s) 239
SsiI CCGC 3 cut(s) 44, 599, 677
SspMI CTAG 3 cut(s) 71, 603, 772
SstI GAGCTC 1 cut(s) 520
StuI AGGCCT 1 cut(s) 239
StyD4I CCNGG 2 cut(s) 406, 669
StyI CCWWGG 4 cut(s) 259, 345, 537, 602
TaaI ACNGT 3 cut(s) 115, 147, 389
TaqI TCGA 5 cut(s) 149, 207, 339, 620, 738
TaqII GACCGA 1 cut(s) 79
TasI AATT 3 cut(s) 152, 503, 633
TatI WGTACW 1 cut(s) 559
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 2 cut(s) 67, 204
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 2 cut(s) 392, 516
TspDTI ATGAA 1 cut(s) 117
TspGWI ACGGA 2 cut(s) 570, 734
TspRI CASTG 2 cut(s) 392, 516
Tth111I GACNNNGTC 1 cut(s) 587
VpaK11BI GGWCC 5 cut(s) 62, 78, 596, 667, 726
XapI RAATTY 2 cut(s) 152, 503
XceI RCATGY 1 cut(s) 575
XmaJI CCTAGG 1 cut(s) 602
XmiI GTMKAC 1 cut(s) 282
XspI CTAG 3 cut(s) 71, 603, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.