MD02G1185300.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013082
Physical Location & Seq
Forward (+)
16826500 .. 16831141
4642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1185300.v1.1.491

Sequence Viewer

Length: 1509 bp
ATGAGGGGTTGGAAAAATCTTGGAGTTGTTGAGACCATCTACGAGGAAGAAGACTATGAATTCTCTTCGGCTTCTCCTTCTCTCTCCCCTGCCCTCTCTTCTCCTTCAACTCCCCTCCACTCCAGAGTAGAATCATGGTCTCGGGCAACAGGGCAAAAAGCGGAAGTATTAATCCGAGTTCAGGGAAAATGTTTCCATCTGCACAAGGATCCTCTGACATCAAGAAGCACATATTTAAAACGTCAGCTAACGGAAATAGTCTCAGATTTCACGATGCCTTTAAATATTTCAGCCGAAACATTCACTGTCGTGGCCGAATTCTGCTACGGAGCTCACCTCGTCATAACGCTGTTCAACGTCGCTGCTCTAAGGACAGCAGCTGAATTGCTTGAGATGACGGAGACCGACAACAATGGCGATGACAACTTGCTCCACTTAACAGACACCTACTTCCGCCAAGTAGTTGCCGTTAATGGAGATTACGCATCTGTTGTCTTCCGTTCGTGTCTATCTTTGCTTCCTGAAGCAGAAACAGCAGCGTTCCTTGTTAGCAGGTGCATTGAGGCGTTGGCTCTGTCGAGCGAGGGTGACAGCGGTGCTGATTGGTTGAACGAGGTTATAACGGTGTGCCCAGAGGATTTTCAAATAGTTGCGGAGGCCATTCAACGCTGTTTTGGAAACCACGACGTTGTTTATAAGCTCGTTGATCTTTATCTTATGAGATACAGTGGAAAGATTACAGAGGATGAGAAAACTCAAATATGCAGTTCAATTGACTGCAGCAAGCTCTCACCACAACTCCTCCTTGAGGCAGTCCGAAACCCCATAATGCCCCTCCGGTTCATAGTCCGAGCCATGTTGATTGAGCAGCTCAACACTCGCCGCACCATTTTCTCCGCAGCAGCAGCAGTAACTGATCACACCCAAATCCAACCTCATCACCGCGACCATGATGGTCATCATCACCGCGACAATAGCAAACAAATAGAGCCCATCGCAGTCAACACTCTAGGCTCTCTCCTTCAACGTGACACAGTGCACCGCCAATCTGCGCATCTCAAGGCCACAATGGCGGCCACAAGCTCGCGGATACAAAGTTTGGAGGAAGAACTTAATGGCATGAAGATGTTGCTGCACGAGTGTCATGAGAAACAGGAAAGAAACATATTAATGGACTCGGGGCGTAGGTCTTCGAGCTTCCATTTTAGCACTAGTGAGAACAATAAGATTGTAAAGGGTGACAAAGCATCTGCTTCTTCGGCTAGTTTTCGGATTATCACTAGTAAGGGATCGGTGGAGACAACTGGTGGGACTTCTAATTGGTCTTCTTCTTGCAAGGGGACTCCGAGATCGACCAAGAAAATTAGTGAGAGGTTGATAATTGGATTGAAGAAGGCGTTTAGAGTTTCGGGTTCGGCTAAAAAGCTCGTCAAGGAGAACAATGTCTATGGGGATACTGGACGTGGATTCGAAGACGGCATTGCTAAAGATATTATGGTGATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

503

Amino Acids

55.76

Weight (kDa)

6.53

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 48 - 135 6.1e-07 BTB/POZ domain
NPH3 PF03000 211 - 265 2.5e-07 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013082)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49900 AT3G49900
fragaria_vesca FvH4_1g17650 FvH4_1g17650
malus_domestica MD02G1185300.v1.1
prunus_persica Prupe.6G221500_v2.0.a1
pyrus_communis pycom02g15020
rosa_chinensis RchiOBHm_Chr2g0107971
rosa_laevigata RLG00000017630
rosa_multiflora Rmu_co8394919.1_g000001 Rmu_sc0001484.1_g000029
rosa_roxburghii Rroxscaffold_2G00135770
rosa_rugosa Rorug02G0149100
rosa_samantha Rh2AG198400 Rh2BG211200 Rh2CG203000 Rh2DG206000
rosa_wichuraiana Rw2G015740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 620, 696
AarI CACCTGC 1 cut(s) 543
Acc16I TGCGCA 1 cut(s) 1053
Acc36I ACCTGC 1 cut(s) 543
AccII CGCG 3 cut(s) 945, 969, 1087
AclWI GGATC 3 cut(s) 203, 216, 1297
AcoI YGGCCR 2 cut(s) 312, 1074
AcsI RAATTY 2 cut(s) 59, 317
AcuI CTGAAG 1 cut(s) 543
AfiI CCNNNNNNNGG 2 cut(s) 181, 808
AgsI TTSAA 8 cut(s) 108, 355, 610, 644, 665, 771, 1025, 1390
AhlI ACTAGT 2 cut(s) 1211, 1280
AjiI CACGTC 1 cut(s) 1463
AleI CACNNNNGTG 1 cut(s) 308
AluBI AGCT 9 cut(s) 247, 332, 380, 700, 787, 871, 1083, 1197, 1426
AluI AGCT 9 cut(s) 247, 332, 380, 700, 787, 871, 1083, 1197, 1426
Alw21I GWGCWC 2 cut(s) 334, 1041
Alw26I GTCTC 5 cut(s) 26, 144, 265, 395, 1292
Alw44I GTGCAC 1 cut(s) 1037
AlwI GGATC 3 cut(s) 203, 216, 1297
AlwNI CAGNNNCTG 2 cut(s) 380, 914
Ama87I CYCGRG 2 cut(s) 141, 1177
AoxI GGCC 4 cut(s) 312, 657, 1062, 1074
ApaLI GTGCAC 1 cut(s) 1037
ApeKI GCWGC 9 cut(s) 362, 377, 536, 780, 868, 899, 902, 905, 1132
ApoI RAATTY 2 cut(s) 59, 317
AseI ATTAAT 2 cut(s) 170, 1169
AspLEI GCGC 1 cut(s) 1054
AsuHPI GGTGA 6 cut(s) 326, 599, 783, 932, 956, 1250
AsuII TTCGAA 1 cut(s) 1470
AvaI CYCGRG 2 cut(s) 141, 1177
BaeGI GKGCMC 2 cut(s) 632, 1041
BamHI GGATCC 1 cut(s) 208
BanII GRGCYC 2 cut(s) 334, 993
BauI CACGAG 1 cut(s) 1136
BbsI GAAGAC 5 cut(s) 57, 487, 1182, 1317, 1479
Bbv12I GWGCWC 2 cut(s) 334, 1041
BbvI GCAGC 9 cut(s) 349, 389, 548, 792, 880, 911, 914, 917, 1119
BccI CCATC 4 cut(s) 44, 204, 947, 1001
BceAI ACGGC 2 cut(s) 452, 1492
BciVI GTATCC 2 cut(s) 1083, 1447
BclI TGATCA 2 cut(s) 916, 1500
BcoDI GTCTC 5 cut(s) 26, 144, 265, 395, 1292
BcuI ACTAGT 2 cut(s) 1211, 1280
BfaI CTAG 4 cut(s) 1010, 1212, 1263, 1281
BfmI CTRYAG 1 cut(s) 778
BfuAI ACCTGC 1 cut(s) 543
BfuI GTATCC 2 cut(s) 1083, 1447
BglI GCCNNNNNGGC 1 cut(s) 1070
BmeT110I CYCGRG 2 cut(s) 141, 1177
BmgBI CACGTC 1 cut(s) 1463
BmiI GGNNCC 1 cut(s) 210
BmsI GCATC 4 cut(s) 264, 494, 1063, 1256
BpiI GAAGAC 5 cut(s) 57, 487, 1182, 1317, 1479
BplI GAGNNNNNCTC 2 cut(s) 321, 353
BpmI CTGGAG 1 cut(s) 106
Bpu14I TTCGAA 1 cut(s) 1470
BpuEI CTTGAG 3 cut(s) 410, 827, 1043
BsaBI GATNNNNATC 2 cut(s) 711, 957
BsaI GGTCTC 3 cut(s) 26, 144, 395
BsaWI WCCGGW 1 cut(s) 837
BsaXI ACNNNNNCTCC 4 cut(s) 783, 786, 813, 816
Bsc4I CCNNNNNNNGG 2 cut(s) 181, 808
Bse1I ACTGG 2 cut(s) 1309, 1462
Bse3DI GCAATG 1 cut(s) 1479
Bse8I GATNNNNATC 2 cut(s) 711, 957
BseGI GGATG 1 cut(s) 751
BseJI GATNNNNATC 2 cut(s) 711, 957
BseLI CCNNNNNNNGG 2 cut(s) 181, 808
BseMI GCAATG 1 cut(s) 1479
BseMII CTCAG 1 cut(s) 276
BseNI ACTGG 2 cut(s) 1309, 1462
BseRI GAGGAG 1 cut(s) 791
BseSI GKGCMC 2 cut(s) 632, 1041
BseXI GCAGC 9 cut(s) 349, 389, 548, 792, 880, 911, 914, 917, 1119
BsgI GTGCAG 2 cut(s) 185, 1118
Bsh1236I CGCG 3 cut(s) 945, 969, 1087
BshFI GGCC 4 cut(s) 314, 659, 1064, 1076
BsiHKAI GWGCWC 2 cut(s) 334, 1041
BsiHKCI CYCGRG 2 cut(s) 141, 1177
BsiSI CCGG 1 cut(s) 838
BslFI GGGAC 2 cut(s) 1324, 1354
BslI CCNNNNNNNGG 2 cut(s) 181, 808
BsmAI GTCTC 5 cut(s) 26, 144, 265, 395, 1292
BsmFI GGGAC 2 cut(s) 1324, 1354
BsnI GGCC 4 cut(s) 314, 659, 1064, 1076
Bso31I GGTCTC 3 cut(s) 26, 144, 395
BsoBI CYCGRG 2 cut(s) 141, 1177
Bsp119I TTCGAA 1 cut(s) 1470
Bsp1286I GDGCHC 4 cut(s) 334, 632, 993, 1041
Bsp143I GATC 6 cut(s) 208, 706, 916, 1289, 1349, 1500
BspANI GGCC 4 cut(s) 314, 659, 1064, 1076
BspCNI CTCAG 1 cut(s) 275
BspFNI CGCG 3 cut(s) 945, 969, 1087
BspHI TCATGA 1 cut(s) 1144
BspLI GGNNCC 1 cut(s) 210
BspMAI CTGCAG 1 cut(s) 782
BspMI ACCTGC 1 cut(s) 543
BspPI GGATC 3 cut(s) 203, 216, 1297
BspT104I TTCGAA 1 cut(s) 1470
BspTNI GGTCTC 3 cut(s) 26, 144, 395
BsrDI GCAATG 1 cut(s) 1479
BsrI ACTGG 2 cut(s) 1309, 1462
BssMI GATC 6 cut(s) 208, 706, 916, 1289, 1349, 1500
BssSI CACGAG 1 cut(s) 1136
Bst2BI CACGAG 1 cut(s) 1136
Bst4CI ACNGT 4 cut(s) 307, 625, 728, 1036
Bst6I CTCTTC 2 cut(s) 70, 103
BstBI TTCGAA 1 cut(s) 1470
BstC8I GCNNGC 2 cut(s) 785, 1085
BstDEI CTNAG 2 cut(s) 262, 368
BstENI CCTNNNNNAGG 1 cut(s) 806
BstF5I GGATG 1 cut(s) 751
BstFNI CGCG 3 cut(s) 945, 969, 1087
BstHHI GCGC 1 cut(s) 1054
BstKTI GATC 6 cut(s) 211, 709, 919, 1292, 1352, 1503
BstMAI GTCTC 5 cut(s) 26, 144, 265, 395, 1292
BstMBI GATC 6 cut(s) 208, 706, 916, 1289, 1349, 1500
BstMWI GCNNNNNNNGC 5 cut(s) 533, 905, 975, 1070, 1259
BstSFI CTRYAG 1 cut(s) 778
BstSLI GKGCMC 2 cut(s) 632, 1041
BstUI CGCG 3 cut(s) 945, 969, 1087
BstV1I GCAGC 9 cut(s) 349, 389, 548, 792, 880, 911, 914, 917, 1119
BstV2I GAAGAC 5 cut(s) 57, 487, 1182, 1317, 1479
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BsuI GTATCC 2 cut(s) 1083, 1447
BsuRI GGCC 4 cut(s) 314, 659, 1064, 1076
BtgZI GCGATG 2 cut(s) 432, 979
BtrI CACGTC 1 cut(s) 1463
BtsCI GGATG 1 cut(s) 751
BtsIMutI CAGTG 3 cut(s) 303, 733, 1041
BveI ACCTGC 1 cut(s) 543
Cac8I GCNNGC 2 cut(s) 785, 1085
CaiI CAGNNNCTG 2 cut(s) 380, 914
CciI TCATGA 1 cut(s) 1144
CfoI GCGC 1 cut(s) 1054
CviAII CATG 5 cut(s) 135, 856, 950, 1120, 1145
DdeI CTNAG 2 cut(s) 262, 368
DpnI GATC 6 cut(s) 210, 708, 918, 1291, 1351, 1502
DpnII GATC 6 cut(s) 208, 706, 916, 1289, 1349, 1500
DraI TTTAAA 2 cut(s) 237, 282
EaeI YGGCCR 2 cut(s) 312, 1074
Eam1104I CTCTTC 2 cut(s) 70, 103
EarI CTCTTC 2 cut(s) 70, 103
EciI GGCGGA 1 cut(s) 443
Ecl136II GAGCTC 1 cut(s) 332
Eco24I GRGCYC 2 cut(s) 334, 993
Eco31I GGTCTC 3 cut(s) 26, 144, 395
Eco53kI GAGCTC 1 cut(s) 332
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 2 cut(s) 141, 1177
EcoICRI GAGCTC 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 806
EcoRI GAATTC 2 cut(s) 59, 317
EcoT38I GRGCYC 2 cut(s) 334, 993
FaeI CATG 5 cut(s) 138, 859, 953, 1123, 1148
FaqI GGGAC 2 cut(s) 1324, 1354
FatI CATG 5 cut(s) 134, 855, 949, 1119, 1144
FbaI TGATCA 2 cut(s) 916, 1500
FokI GGATG 1 cut(s) 758
FriOI GRGCYC 2 cut(s) 334, 993
FspBI CTAG 4 cut(s) 1010, 1212, 1263, 1281
FspI TGCGCA 1 cut(s) 1053
GlaI GCGC 1 cut(s) 1053
GsuI CTGGAG 1 cut(s) 106
HaeIII GGCC 4 cut(s) 314, 659, 1064, 1076
HapII CCGG 1 cut(s) 838
HhaI GCGC 1 cut(s) 1054
Hin1II CATG 5 cut(s) 138, 859, 953, 1123, 1148
Hin6I GCGC 1 cut(s) 1052
HinP1I GCGC 1 cut(s) 1052
HincII GTYRAC 1 cut(s) 1003
HindII GTYRAC 1 cut(s) 1003
HinfI GANTC 4 cut(s) 131, 1175, 1342, 1467
HpaII CCGG 1 cut(s) 838
HphI GGTGA 6 cut(s) 326, 599, 783, 932, 956, 1250
Hpy166II GTNNAC 2 cut(s) 1003, 1039
Hpy188I TCNGA 7 cut(s) 176, 216, 265, 818, 851, 1272, 1347
Hpy188III TCNNGA 5 cut(s) 123, 222, 271, 521, 1145
Hpy8I GTNNAC 2 cut(s) 1003, 1039
Hpy99I CGWCG 2 cut(s) 362, 689
HpyAV CCTTC 4 cut(s) 87, 114, 1031, 1387
HpyCH4III ACNGT 4 cut(s) 307, 625, 728, 1036
HpyCH4IV ACGT 5 cut(s) 241, 357, 687, 1027, 1462
HpyCH4V TGCA 7 cut(s) 202, 558, 765, 780, 1039, 1135, 1335
HpyF10VI GCNNNNNNNGC 5 cut(s) 533, 905, 975, 1070, 1259
HpyF3I CTNAG 2 cut(s) 262, 368
HpySE526I ACGT 5 cut(s) 241, 357, 687, 1027, 1462
Hsp92II CATG 5 cut(s) 138, 859, 953, 1123, 1148
HspAI GCGC 1 cut(s) 1052
Ksp22I TGATCA 2 cut(s) 916, 1500
Kzo9I GATC 6 cut(s) 208, 706, 916, 1289, 1349, 1500
LmnI GCTCC 2 cut(s) 329, 435
Lsp1109I GCAGC 9 cut(s) 349, 389, 548, 792, 880, 911, 914, 917, 1119
LweI GCATC 4 cut(s) 264, 494, 1063, 1256
MaeI CTAG 4 cut(s) 1010, 1212, 1263, 1281
MaeII ACGT 5 cut(s) 241, 357, 687, 1027, 1462
MaeIII GTNAC 4 cut(s) 587, 910, 1028, 1238
MalI GATC 6 cut(s) 210, 708, 918, 1291, 1351, 1502
MboI GATC 6 cut(s) 208, 706, 916, 1289, 1349, 1500
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 4 cut(s) 334, 632, 993, 1041
MluCI AATT 7 cut(s) 59, 317, 383, 771, 1318, 1362, 1380
MlyI GAGTC 2 cut(s) 1169, 1336
MmeI TCCRAC 1 cut(s) 955
MseI TTAA 7 cut(s) 170, 236, 281, 437, 471, 1113, 1169
MslI CAYNNNNRTG 3 cut(s) 308, 1124, 1169
MspA1I CMGCKG 2 cut(s) 380, 594
MspI CCGG 1 cut(s) 838
MunI CAATTG 1 cut(s) 771
MvnI CGCG 3 cut(s) 945, 969, 1087
MwoI GCNNNNNNNGC 5 cut(s) 533, 905, 975, 1070, 1259
NdeII GATC 6 cut(s) 208, 706, 916, 1289, 1349, 1500
NlaIII CATG 5 cut(s) 138, 859, 953, 1123, 1148
NlaIV GGNNCC 1 cut(s) 210
NmuCI GTSAC 3 cut(s) 587, 1028, 1238
NsbI TGCGCA 1 cut(s) 1053
NspV TTCGAA 1 cut(s) 1470
OliI CACNNNNGTG 1 cut(s) 308
PagI TCATGA 1 cut(s) 1144
PaqCI CACCTGC 1 cut(s) 543
PfeI GAWTC 2 cut(s) 131, 1467
PleI GAGTC 2 cut(s) 1169, 1336
PpsI GAGTC 2 cut(s) 1169, 1336
PshBI ATTAAT 2 cut(s) 170, 1169
PsiI TTATAA 2 cut(s) 620, 696
Psp124BI GAGCTC 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 210
PstI CTGCAG 1 cut(s) 782
PstNI CAGNNNCTG 2 cut(s) 380, 914
PsuI RGATCY 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 380
RseI CAYNNNNRTG 3 cut(s) 308, 1124, 1169
SacI GAGCTC 1 cut(s) 334
SaqAI TTAA 7 cut(s) 170, 236, 281, 437, 471, 1113, 1169
Sau3AI GATC 6 cut(s) 208, 706, 916, 1289, 1349, 1500
SchI GAGTC 2 cut(s) 1169, 1336
SduI GDGCHC 4 cut(s) 334, 632, 993, 1041
SfaNI GCATC 4 cut(s) 264, 494, 1063, 1256
SfcI CTRYAG 1 cut(s) 778
SfuI TTCGAA 1 cut(s) 1470
SmiMI CAYNNNNRTG 3 cut(s) 308, 1124, 1169
SmlI CTYRAG 3 cut(s) 389, 806, 1058
SmoI CTYRAG 3 cut(s) 389, 806, 1058
SpeI ACTAGT 2 cut(s) 1211, 1280
Sse9I AATT 7 cut(s) 59, 317, 383, 771, 1318, 1362, 1380
SspI AATATT 1 cut(s) 286
SspMI CTAG 4 cut(s) 1010, 1212, 1263, 1281
SstI GAGCTC 1 cut(s) 334
TaaI ACNGT 4 cut(s) 307, 625, 728, 1036
TaiI ACGT 5 cut(s) 244, 360, 690, 1030, 1465
TaqI TCGA 4 cut(s) 578, 1193, 1352, 1470
TaqII GACCGA 1 cut(s) 419
TasI AATT 7 cut(s) 59, 317, 383, 771, 1318, 1362, 1380
TauI GCSGC 2 cut(s) 885, 1076
TfiI GAWTC 2 cut(s) 131, 1467
Tru1I TTAA 7 cut(s) 170, 236, 281, 437, 471, 1113, 1169
Tru9I TTAA 7 cut(s) 170, 236, 281, 437, 471, 1113, 1169
TscAI CASTG 3 cut(s) 310, 733, 1041
TseFI GTSAC 3 cut(s) 587, 1028, 1238
TseI GCWGC 9 cut(s) 362, 377, 536, 780, 868, 899, 902, 905, 1132
Tsp45I GTSAC 3 cut(s) 587, 1028, 1238
TspDTI ATGAA 3 cut(s) 72, 832, 1136
TspGWI ACGGA 4 cut(s) 266, 342, 413, 488
TspRI CASTG 3 cut(s) 310, 733, 1041
VneI GTGCAC 1 cut(s) 1037
VspI ATTAAT 2 cut(s) 170, 1169
XagI CCTNNNNNAGG 1 cut(s) 806
XapI RAATTY 2 cut(s) 59, 317
XspI CTAG 4 cut(s) 1010, 1212, 1263, 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.