pycom02g15020

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013082
Physical Location & Seq
Forward (+)
11733602 .. 11737131
3530 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15020.1

Sequence Viewer

Length: 1548 bp
ATGAGGGGTTGGCTGCTGCTAACTGCTAAGCGGCAGGCAATGAGGGGTTGGAAAAATCTTGGAGTTGTTGAGACCATCTACGAGGAAGAAGACTATGAATTCTCTTCGGCTTCTCCTTCTCTCTCCCCTGCCCTCTCTTCTCCTTCCACTCCCCTCCACTCCAGAGTAGAATCATGGTCTCGGGCAACAGGGCAAAAAGCGGAAGTATTAATCCGAGTTCAGGGAAAATGTTTCCATCTGCACAAGGATCCTCTGACATCAAGAAGCACATATTTAAAACGTCAGCTAACGGAAATAGTCTCAGATTTTACGATGCCTTTAAACATTTCAGCCGAAACATTCACTCTCGTAGCCGAATTCTGCTACGGAGCTCACCTTGTCATAACGCTGTTCAACGTCGCTGCTCTAAGGACAGCAGCCGAATTGCTTGAGATGACGGAGACCGACTACAATGGCGATGACAACTTGCTCCACTTAGCAGACACCTACTTCCGCCAAGTAGTTGCCGTTAATAGAGATTACGCATCTGTTGTCTTCCGTTCGTGTCTGTCTTTGCTTCCTGAAGCAGAAACAGCAGCATTCCTTGTTAGCAGGTGCATTGAGGCGTTGGCTCTGTCGAGCGAGGGTGACAACGGTGCTGATTGGTTGAACGAGGTTATAATGGTGTGCCCAGAGGATTTTCAAATAGTTGCGGAGGCCATCCAACGCTGTTTTGGAAACCACGATGTTGTTTATAAGCTCGTTGATCTTTATCTTATGAGATACAGTGGAAAGATTACAGAGGATGAGAAAACTCAAATATGCAGTTCAATTGACTGCAGCAAGCTCTCACCACAACTCCTCCTTGAGGCAGTCCAAAACCCCATAATGCCCCTCCGGTTCATAGTCCGAGCCATGTTGATTGAGCAGCTCAAAACTCGCCACACCATTTTCTCCGCAGCAGCAGCAGTAACTGATCATACCCAAATCCAACCTCATCACCGCGACCATGATGGTCATCATCACCGTGACAATAGCAAACAAATAGAGCCCGTCGCAGTCAACACGCTAGGCTCTCTCCTTCAACGTGACACAGTGCACCGCCAATCTGCGCATCTCAAGGCCACAATGGCGGCCACAAGCTCGCGGATACAAAGTTTGGAGGAAGAACTTAATGGCATGAAGATGTTGCTGCACGAGTGTCATGAGAAACAGGAAACAAACATATTAATGGACTCGGGGCGTAGGTCTTCGAGCTTCCATTTTGGCACTAGTGAGAACAATAAGATTGTAAAGGGTGACAAAGCATCTGCTTCTTCGGCTAGTTTTCGGATTATCACTAGTAAAGGATCGGCCGAGACAACTGGAGGGACTTCTAATTGGTCTTCTTCTTGCAAGGGGACTCCGAGATCCACCAAGAAAATTAGTGAGAGGTTGATAATTGGATTGAAGAAGGCGTTTAGGGTTTCGGGTTCGGCTAAAAAGCTCGTCAAGGAGAGCAATGTCTATGGGGATACTGGACGTGGATTCGAAGACGGCATCGCTAAAGATGTTATGGTGATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

516

Amino Acids

57.27

Weight (kDa)

6.84

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 61 - 147 3.6e-07 BTB/POZ domain
NPH3 PF03000 224 - 278 2.8e-07 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013082)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49900 AT3G49900
fragaria_vesca FvH4_1g17650 FvH4_1g17650
malus_domestica MD02G1185300.v1.1
prunus_persica Prupe.6G221500_v2.0.a1
pyrus_communis pycom02g15020
rosa_chinensis RchiOBHm_Chr2g0107971
rosa_laevigata RLG00000017630
rosa_multiflora Rmu_co8394919.1_g000001 Rmu_sc0001484.1_g000029
rosa_roxburghii Rroxscaffold_2G00135770
rosa_rugosa Rorug02G0149100
rosa_samantha Rh2AG198400 Rh2BG211200 Rh2CG203000 Rh2DG206000
rosa_wichuraiana Rw2G015740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 659, 735
AarI CACCTGC 1 cut(s) 582
Acc16I TGCGCA 1 cut(s) 1092
Acc36I ACCTGC 1 cut(s) 582
AccII CGCG 2 cut(s) 984, 1126
AciI CCGC 9 cut(s) 31, 200, 493, 692, 936, 982, 1081, 1112, 1126
AclWI GGATC 4 cut(s) 242, 255, 1336, 1383
AcoI YGGCCR 2 cut(s) 1113, 1332
AcsI RAATTY 2 cut(s) 98, 356
AcuI CTGAAG 1 cut(s) 582
AfiI CCNNNNNNNGG 2 cut(s) 220, 847
AgsI TTSAA 6 cut(s) 394, 649, 683, 810, 1064, 1429
AhlI ACTAGT 2 cut(s) 1250, 1319
AjiI CACGTC 1 cut(s) 1502
AluBI AGCT 8 cut(s) 286, 371, 739, 826, 910, 1122, 1236, 1465
AluI AGCT 8 cut(s) 286, 371, 739, 826, 910, 1122, 1236, 1465
Alw21I GWGCWC 2 cut(s) 373, 1080
Alw26I GTCTC 5 cut(s) 65, 183, 304, 434, 1331
Alw44I GTGCAC 1 cut(s) 1076
AlwI GGATC 4 cut(s) 242, 255, 1336, 1383
AlwNI CAGNNNCTG 1 cut(s) 953
Ama87I CYCGRG 2 cut(s) 180, 1216
AoxI GGCC 4 cut(s) 696, 1101, 1113, 1332
ApaLI GTGCAC 1 cut(s) 1076
ApoI RAATTY 2 cut(s) 98, 356
AseI ATTAAT 2 cut(s) 209, 1208
AspLEI GCGC 1 cut(s) 1093
AsuHPI GGTGA 6 cut(s) 365, 638, 822, 971, 995, 1289
AsuII TTCGAA 1 cut(s) 1509
AvaI CYCGRG 2 cut(s) 180, 1216
BaeGI GKGCMC 2 cut(s) 671, 1080
BamHI GGATCC 1 cut(s) 247
BanII GRGCYC 2 cut(s) 373, 1032
BauI CACGAG 1 cut(s) 1175
BbsI GAAGAC 5 cut(s) 96, 526, 1221, 1356, 1518
Bbv12I GWGCWC 2 cut(s) 373, 1080
BccI CCATC 4 cut(s) 83, 243, 707, 986
BceAI ACGGC 2 cut(s) 491, 1531
BciVI GTATCC 2 cut(s) 1122, 1486
BclI TGATCA 2 cut(s) 955, 1539
BcoDI GTCTC 5 cut(s) 65, 183, 304, 434, 1331
BcuI ACTAGT 2 cut(s) 1250, 1319
BfaI CTAG 4 cut(s) 1049, 1251, 1302, 1320
BfmI CTRYAG 1 cut(s) 817
BfuAI ACCTGC 1 cut(s) 582
BfuI GTATCC 2 cut(s) 1122, 1486
BglI GCCNNNNNGGC 1 cut(s) 1109
BlpI GCTNAGC 1 cut(s) 27
BmeT110I CYCGRG 2 cut(s) 180, 1216
BmgBI CACGTC 1 cut(s) 1502
BmiI GGNNCC 1 cut(s) 249
BmsI GCATC 5 cut(s) 303, 533, 1102, 1295, 1527
BpiI GAAGAC 5 cut(s) 96, 526, 1221, 1356, 1518
BpmI CTGGAG 2 cut(s) 145, 1365
Bpu1102I GCTNAGC 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 1509
BpuEI CTTGAG 3 cut(s) 449, 866, 1082
BsaBI GATNNNNATC 2 cut(s) 750, 996
BsaI GGTCTC 3 cut(s) 65, 183, 434
BsaWI WCCGGW 1 cut(s) 876
BsaXI ACNNNNNCTCC 4 cut(s) 822, 825, 852, 855
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 847
Bse1I ACTGG 2 cut(s) 1348, 1501
Bse3DI GCAATG 2 cut(s) 45, 1486
Bse8I GATNNNNATC 2 cut(s) 750, 996
BseGI GGATG 2 cut(s) 699, 790
BseJI GATNNNNATC 2 cut(s) 750, 996
BseLI CCNNNNNNNGG 2 cut(s) 220, 847
BseMI GCAATG 2 cut(s) 45, 1486
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 2 cut(s) 1348, 1501
BseRI GAGGAG 1 cut(s) 830
BseSI GKGCMC 2 cut(s) 671, 1080
BseX3I CGGCCG 1 cut(s) 1332
BsgI GTGCAG 2 cut(s) 224, 1157
Bsh1236I CGCG 2 cut(s) 984, 1126
Bsh1285I CGRYCG 1 cut(s) 1335
BshFI GGCC 4 cut(s) 698, 1103, 1115, 1334
BsiEI CGRYCG 1 cut(s) 1335
BsiHKAI GWGCWC 2 cut(s) 373, 1080
BsiHKCI CYCGRG 2 cut(s) 180, 1216
BsiSI CCGG 1 cut(s) 877
BslFI GGGAC 2 cut(s) 1363, 1393
BslI CCNNNNNNNGG 2 cut(s) 220, 847
BsmAI GTCTC 5 cut(s) 65, 183, 304, 434, 1331
BsmFI GGGAC 2 cut(s) 1363, 1393
BsmI GAATGC 1 cut(s) 578
BsnI GGCC 4 cut(s) 698, 1103, 1115, 1334
Bso31I GGTCTC 3 cut(s) 65, 183, 434
BsoBI CYCGRG 2 cut(s) 180, 1216
Bsp119I TTCGAA 1 cut(s) 1509
Bsp1286I GDGCHC 4 cut(s) 373, 671, 1032, 1080
Bsp143I GATC 6 cut(s) 247, 745, 955, 1328, 1388, 1539
Bsp1720I GCTNAGC 1 cut(s) 27
BspACI CCGC 9 cut(s) 31, 200, 493, 692, 936, 982, 1081, 1112, 1126
BspANI GGCC 4 cut(s) 698, 1103, 1115, 1334
BspCNI CTCAG 1 cut(s) 314
BspFNI CGCG 2 cut(s) 984, 1126
BspHI TCATGA 1 cut(s) 1183
BspLI GGNNCC 1 cut(s) 249
BspMAI CTGCAG 1 cut(s) 821
BspMI ACCTGC 1 cut(s) 582
BspPI GGATC 4 cut(s) 242, 255, 1336, 1383
BspT104I TTCGAA 1 cut(s) 1509
BspTNI GGTCTC 3 cut(s) 65, 183, 434
BsrDI GCAATG 2 cut(s) 45, 1486
BsrI ACTGG 2 cut(s) 1348, 1501
BssMI GATC 6 cut(s) 247, 745, 955, 1328, 1388, 1539
BssSI CACGAG 1 cut(s) 1175
Bst2BI CACGAG 1 cut(s) 1175
Bst4CI ACNGT 4 cut(s) 635, 767, 1007, 1075
Bst6I CTCTTC 2 cut(s) 109, 142
BstBI TTCGAA 1 cut(s) 1509
BstC8I GCNNGC 3 cut(s) 36, 824, 1124
BstDEI CTNAG 4 cut(s) 27, 301, 407, 475
BstENI CCTNNNNNAGG 1 cut(s) 845
BstF5I GGATG 2 cut(s) 699, 790
BstFNI CGCG 2 cut(s) 984, 1126
BstHHI GCGC 1 cut(s) 1093
BstKTI GATC 6 cut(s) 250, 748, 958, 1331, 1391, 1542
BstMAI GTCTC 5 cut(s) 65, 183, 304, 434, 1331
BstMBI GATC 6 cut(s) 247, 745, 955, 1328, 1388, 1539
BstMCI CGRYCG 1 cut(s) 1335
BstMWI GCNNNNNNNGC 4 cut(s) 572, 944, 1109, 1298
BstSFI CTRYAG 1 cut(s) 817
BstSLI GKGCMC 2 cut(s) 671, 1080
BstUI CGCG 2 cut(s) 984, 1126
BstV2I GAAGAC 5 cut(s) 96, 526, 1221, 1356, 1518
BstX2I RGATCY 2 cut(s) 247, 1388
BstYI RGATCY 2 cut(s) 247, 1388
BstZI CGGCCG 1 cut(s) 1332
BsuI GTATCC 2 cut(s) 1122, 1486
BsuRI GGCC 4 cut(s) 698, 1103, 1115, 1334
BtgZI GCGATG 2 cut(s) 471, 1504
BtrI CACGTC 1 cut(s) 1502
BtsCI GGATG 2 cut(s) 699, 790
BtsIMutI CAGTG 2 cut(s) 772, 1080
BveI ACCTGC 1 cut(s) 582
Cac8I GCNNGC 3 cut(s) 36, 824, 1124
CaiI CAGNNNCTG 1 cut(s) 953
CciI TCATGA 1 cut(s) 1183
CfoI GCGC 1 cut(s) 1093
CviAII CATG 5 cut(s) 174, 895, 989, 1159, 1184
DdeI CTNAG 4 cut(s) 27, 301, 407, 475
DpnI GATC 6 cut(s) 249, 747, 957, 1330, 1390, 1541
DpnII GATC 6 cut(s) 247, 745, 955, 1328, 1388, 1539
DraI TTTAAA 2 cut(s) 276, 321
EaeI YGGCCR 2 cut(s) 1113, 1332
EagI CGGCCG 1 cut(s) 1332
Eam1104I CTCTTC 2 cut(s) 109, 142
EarI CTCTTC 2 cut(s) 109, 142
EciI GGCGGA 1 cut(s) 482
Ecl136II GAGCTC 1 cut(s) 371
EclXI CGGCCG 1 cut(s) 1332
Eco24I GRGCYC 2 cut(s) 373, 1032
Eco31I GGTCTC 3 cut(s) 65, 183, 434
Eco52I CGGCCG 1 cut(s) 1332
Eco53kI GAGCTC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 582
Eco88I CYCGRG 2 cut(s) 180, 1216
EcoICRI GAGCTC 1 cut(s) 371
EcoNI CCTNNNNNAGG 1 cut(s) 845
EcoRI GAATTC 2 cut(s) 98, 356
EcoT38I GRGCYC 2 cut(s) 373, 1032
FaeI CATG 5 cut(s) 177, 898, 992, 1162, 1187
FaqI GGGAC 2 cut(s) 1363, 1393
FatI CATG 5 cut(s) 173, 894, 988, 1158, 1183
FbaI TGATCA 2 cut(s) 955, 1539
FokI GGATG 2 cut(s) 686, 797
FriOI GRGCYC 2 cut(s) 373, 1032
FspBI CTAG 4 cut(s) 1049, 1251, 1302, 1320
FspI TGCGCA 1 cut(s) 1092
GlaI GCGC 1 cut(s) 1092
GsuI CTGGAG 2 cut(s) 145, 1365
HaeIII GGCC 4 cut(s) 698, 1103, 1115, 1334
HapII CCGG 1 cut(s) 877
HhaI GCGC 1 cut(s) 1093
Hin1II CATG 5 cut(s) 177, 898, 992, 1162, 1187
Hin6I GCGC 1 cut(s) 1091
HinP1I GCGC 1 cut(s) 1091
HincII GTYRAC 1 cut(s) 1042
HindII GTYRAC 1 cut(s) 1042
HinfI GANTC 4 cut(s) 170, 1214, 1381, 1506
HpaII CCGG 1 cut(s) 877
HphI GGTGA 6 cut(s) 365, 638, 822, 971, 995, 1289
Hpy166II GTNNAC 2 cut(s) 1042, 1078
Hpy188I TCNGA 6 cut(s) 215, 255, 304, 890, 1311, 1386
Hpy188III TCNNGA 4 cut(s) 162, 261, 560, 1184
Hpy8I GTNNAC 2 cut(s) 1042, 1078
Hpy99I CGWCG 2 cut(s) 401, 1037
HpyAV CCTTC 4 cut(s) 126, 153, 1070, 1426
HpyCH4III ACNGT 4 cut(s) 635, 767, 1007, 1075
HpyCH4IV ACGT 4 cut(s) 280, 396, 1066, 1501
HpyCH4V TGCA 7 cut(s) 241, 597, 804, 819, 1078, 1174, 1374
HpyF10VI GCNNNNNNNGC 4 cut(s) 572, 944, 1109, 1298
HpyF3I CTNAG 4 cut(s) 27, 301, 407, 475
HpySE526I ACGT 4 cut(s) 280, 396, 1066, 1501
Hsp92II CATG 5 cut(s) 177, 898, 992, 1162, 1187
HspAI GCGC 1 cut(s) 1091
Ksp22I TGATCA 2 cut(s) 955, 1539
Kzo9I GATC 6 cut(s) 247, 745, 955, 1328, 1388, 1539
LmnI GCTCC 2 cut(s) 368, 474
LweI GCATC 5 cut(s) 303, 533, 1102, 1295, 1527
MaeI CTAG 4 cut(s) 1049, 1251, 1302, 1320
MaeII ACGT 4 cut(s) 280, 396, 1066, 1501
MaeIII GTNAC 5 cut(s) 626, 949, 1007, 1067, 1277
MalI GATC 6 cut(s) 249, 747, 957, 1330, 1390, 1541
MboI GATC 6 cut(s) 247, 745, 955, 1328, 1388, 1539
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 2 cut(s) 247, 1388
MhlI GDGCHC 4 cut(s) 373, 671, 1032, 1080
MluCI AATT 7 cut(s) 98, 356, 422, 810, 1357, 1401, 1419
MlyI GAGTC 2 cut(s) 1208, 1375
MmeI TCCRAC 3 cut(s) 29, 727, 994
MseI TTAA 6 cut(s) 209, 275, 320, 510, 1152, 1208
MslI CAYNNNNRTG 3 cut(s) 1005, 1163, 1208
MspI CCGG 1 cut(s) 877
MunI CAATTG 1 cut(s) 810
Mva1269I GAATGC 1 cut(s) 578
MvnI CGCG 2 cut(s) 984, 1126
MwoI GCNNNNNNNGC 4 cut(s) 572, 944, 1109, 1298
NdeII GATC 6 cut(s) 247, 745, 955, 1328, 1388, 1539
NlaIII CATG 5 cut(s) 177, 898, 992, 1162, 1187
NlaIV GGNNCC 1 cut(s) 249
NmeAIII GCCGAG 1 cut(s) 1360
NmuCI GTSAC 4 cut(s) 626, 1007, 1067, 1277
NsbI TGCGCA 1 cut(s) 1092
NspV TTCGAA 1 cut(s) 1509
PagI TCATGA 1 cut(s) 1183
PaqCI CACCTGC 1 cut(s) 582
PctI GAATGC 1 cut(s) 578
PfeI GAWTC 2 cut(s) 170, 1506
PleI GAGTC 2 cut(s) 1208, 1375
PpsI GAGTC 2 cut(s) 1208, 1375
PshBI ATTAAT 2 cut(s) 209, 1208
PsiI TTATAA 2 cut(s) 659, 735
Psp124BI GAGCTC 1 cut(s) 373
PspN4I GGNNCC 1 cut(s) 249
PstI CTGCAG 1 cut(s) 821
PstNI CAGNNNCTG 1 cut(s) 953
PsuI RGATCY 2 cut(s) 247, 1388
RseI CAYNNNNRTG 3 cut(s) 1005, 1163, 1208
SacI GAGCTC 1 cut(s) 373
SaqAI TTAA 6 cut(s) 209, 275, 320, 510, 1152, 1208
Sau3AI GATC 6 cut(s) 247, 745, 955, 1328, 1388, 1539
SchI GAGTC 2 cut(s) 1208, 1375
SduI GDGCHC 4 cut(s) 373, 671, 1032, 1080
SfaNI GCATC 5 cut(s) 303, 533, 1102, 1295, 1527
SfcI CTRYAG 1 cut(s) 817
SfuI TTCGAA 1 cut(s) 1509
SmiMI CAYNNNNRTG 3 cut(s) 1005, 1163, 1208
SmlI CTYRAG 3 cut(s) 428, 845, 1097
SmoI CTYRAG 3 cut(s) 428, 845, 1097
SpeI ACTAGT 2 cut(s) 1250, 1319
Sse9I AATT 7 cut(s) 98, 356, 422, 810, 1357, 1401, 1419
SsiI CCGC 9 cut(s) 31, 200, 493, 692, 936, 982, 1081, 1112, 1126
SspMI CTAG 4 cut(s) 1049, 1251, 1302, 1320
SstI GAGCTC 1 cut(s) 373
TaaI ACNGT 4 cut(s) 635, 767, 1007, 1075
TaiI ACGT 4 cut(s) 283, 399, 1069, 1504
TaqI TCGA 3 cut(s) 617, 1232, 1509
TaqII GACCGA 1 cut(s) 458
TasI AATT 7 cut(s) 98, 356, 422, 810, 1357, 1401, 1419
TauI GCSGC 2 cut(s) 34, 1115
TfiI GAWTC 2 cut(s) 170, 1506
Tru1I TTAA 6 cut(s) 209, 275, 320, 510, 1152, 1208
Tru9I TTAA 6 cut(s) 209, 275, 320, 510, 1152, 1208
TscAI CASTG 2 cut(s) 772, 1080
TseFI GTSAC 4 cut(s) 626, 1007, 1067, 1277
Tsp45I GTSAC 4 cut(s) 626, 1007, 1067, 1277
TspDTI ATGAA 3 cut(s) 111, 871, 1175
TspGWI ACGGA 4 cut(s) 305, 381, 452, 527
TspRI CASTG 2 cut(s) 772, 1080
VneI GTGCAC 1 cut(s) 1076
VspI ATTAAT 2 cut(s) 209, 1208
XagI CCTNNNNNAGG 1 cut(s) 845
XapI RAATTY 2 cut(s) 98, 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 710
XspI CTAG 4 cut(s) 1049, 1251, 1302, 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.