Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0014436

Physical Location & Seq

Genomic Coordinates Forward (+)

16892351 .. 16893874

Gene Structure 1524 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1185900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1524 bp

ATGGATACCTTGTTTAGGCTAGTCAATCTCCAATCTGATAACCATCAATCTTTCACCAACTCAACCACCGCCAGAACCTCAACACCTACAACAACCACCACCACTTCTAGCAGCTCCAGATCCTCCTTCCTCAGTAACCCTCCTCACCCTAATTACCCCCACCAAGACGACGACGTTGGAGAAGAATGCTTCAACTTTTTCATGGATGAAGAAGACTTCTCCTCGTCTTCTTCCAAACACTACTTCCCATCGAACCACCCAACCCCTCCCACCCCAAACCCTCTCCCCACCATCACCACCACGCCCACCCCAGCCGACTTCTCCTTCTCCCCCTCCCTCGTCAACTTCGAGCTCTCCAACGACAAGTGGGCCCCGGAACTCCTCTTGGAGACCGCCAGAGCCATCGCGGACAAGAACAGCTCCCGCGTCCAGCAGCTCATGTGGATGCTCAACGAGCTCGGCAGTCCGTACGGAGACACCGACCAGAAACTCGCCTCCTATTTCCTCCAGGCTTTGTTCAGCCGCATGACCGACTCCGGTGACCGTTGCTACCGAACCCTAATCTCCGCCTCGGAGAAGACGTGTTCCTTCGAGTCCACAAGAAAAACGGTGCTCAAGTTTCAGGAGGTCAGCCCTTGGACCACTTTCGGACACGTGGCGTGCAATGGCGCGATCATGGAGGCTTTTGATGGTGAGCCAAAGCTACACATAATCGACATCAGCAACACTTACTGCACCCAATGGCCGACTCTGCTCGAGGCGCTGGCCACTCGAACTGATGAGACGCCGCACTTGCGTCTCACCACTGTTGTGGCTAACAGAGCCAACGACGGTTCTGGTGGCGCAGCCGCGCAGAAAGTAATGAAGGAAATTGGCGCGAGGATGGAAAAGTTTGCCCGGTTGATGGGCGTGCCTTTCAAATTCAACGCCGTCCATCACTCTGGCGATCTTTCAGAGCTGAATTTATCAGAATTGGATATCAGAGACGATGAGGCCTTGGCAATCAACTGCGTCGGCACTCTACATTCGGTTCAGTCCATCGGAAACCGCCGCGATTATCTTGTTTCGGCGTTTCGGAGCTTGAGGCCAAGGATCATTACTCTTGTTGAGGAAGAGGCTGATCTCGACGTTGGAGTTGACGGGTTTGACTTCGTTCATGGGTTTCAAGAGTGCTTGAGATGGTTTAGGGTTTACTTCGAGGCATTGGACGAGAGCTTTTCCAGGACCAGCAACGAGCGGTTGATACTCGAGCGGGCCGCCGGGCGAGCGGTAGTGGATTTGGTGGCCTGCGCACCTTCGGAGTCGGTGGAGCGGCGCGAGACCGCCACTCGCTGGTCGAGGCGTATGCATGGGGCGGGGTTTAGTCCTGCGATGTTTAGTGATGAGGTGTGTGATGACGTACGCGCCTTGTTGAGGAGGTATAAGGAAGGTTGGGCAATGACACAGTGCACAGATTCCATCGCCGGAATATTCTTGTCGTGGAAGGACCAGCCGGTGGTTTGGGCTAGTGCTTGGAGGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008356 GO:0009889 GO:0009956 GO:0009987 GO:0010468 GO:0010556 GO:0010817 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032350 GO:0032501 GO:0032502 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045786 GO:0045930 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051171 GO:0051252 GO:0051301 GO:0051726 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060255 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098771 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

508

Amino Acids

56.88

Weight (kDa)

5.22

Isoelectric Point (pI)

48.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	126 - 506	6.7e-105	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014436)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37650
fragaria_vesca	FvH4_1g17690
malus_domestica	MD02G1185900.v1.1 MD15G1307800.v1.1
prunus_persica	Prupe.6G220800_v2.0.a1
pyrus_communis	pycom02g15090 pycom15g26030
rosa_chinensis	RchiOBHm_Chr2g0108031
rosa_laevigata	RLG00000017634
rosa_multiflora	Rmu_sc0001484.1_g000021
rosa_roxburghii	Rroxscaffold_2G00135710
rosa_rugosa	Rorug02G0149800
rosa_samantha	Rh2AG198800 Rh2BG211700 Rh2CG203400 Rh2DG206400
rosa_wichuraiana	Rw2G015780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1291
AccB7I	CCANNNNNTGG	2 cut(s)	1332, 1495
AccBSI	CCGCTC	4 cut(s)	1237, 1252, 1268, 1312
AccII	CGCG	8 cut(s)	407, 426, 671, 851, 878, 1053, 1317, 1404
AclWI	GGATC	2 cut(s)	114, 1100
AcoI	YGGCCR	2 cut(s)	743, 765
AcsI	RAATTY	2 cut(s)	920, 961
AcvI	CACGTG	1 cut(s)	655
AcyI	GRCGYC	1 cut(s)	785
AfaI	GTAC	2 cut(s)	470, 1401
AfiI	CCNNNNNNNGG	5 cut(s)	15, 904, 1332, 1413, 1495
AflIII	ACRYGT	2 cut(s)	581, 652
AgsI	TTSAA	4 cut(s)	193, 919, 925, 1166
AjiI	CACGTC	1 cut(s)	582
AjnI	CCWGG	2 cut(s)	507, 1220
AjuI	GAANNNNNNNTTGG	2 cut(s)	227, 259
AleI	CACNNNNGTG	1 cut(s)	809
AluBI	AGCT	9 cut(s)	114, 352, 420, 436, 457, 703, 958, 1080, 1215
AluI	AGCT	9 cut(s)	114, 352, 420, 436, 457, 703, 958, 1080, 1215
Alw21I	GWGCWC	4 cut(s)	354, 459, 615, 1451
Alw26I	GTCTC	6 cut(s)	383, 468, 776, 803, 978, 1313
Alw44I	GTGCAC	1 cut(s)	1447
AlwI	GGATC	2 cut(s)	114, 1100
Ama87I	CYCGRG	2 cut(s)	755, 1247
AoxI	GGCC	8 cut(s)	369, 743, 765, 993, 1085, 1254, 1284, 1517
ApaI	GGGCCC	1 cut(s)	373
ApaLI	GTGCAC	1 cut(s)	1447
ApeKI	GCWGC	3 cut(s)	111, 433, 845
ApoI	RAATTY	2 cut(s)	920, 961
AspLEI	GCGC	8 cut(s)	671, 763, 845, 853, 878, 1292, 1317, 1406
AspS9I	GGNCC	6 cut(s)	369, 370, 639, 1224, 1254, 1486
AsuC2I	CCSGG	3 cut(s)	374, 898, 1261
AsuHPI	GGTGA	6 cut(s)	46, 137, 286, 551, 704, 793
AvaI	CYCGRG	2 cut(s)	755, 1247
AvaII	GGWCC	3 cut(s)	639, 1224, 1486
BaeGI	GKGCMC	2 cut(s)	373, 1451
BalI	TGGCCA	1 cut(s)	767
BanII	GRGCYC	3 cut(s)	354, 373, 459
BbrPI	CACGTG	1 cut(s)	655
BbsI	GAAGAC	3 cut(s)	219, 219, 584
Bbv12I	GWGCWC	4 cut(s)	354, 459, 615, 1451
BbvI	GCAGC	3 cut(s)	123, 445, 857
BceAI	ACGGC	1 cut(s)	914
BcgI	CGANNNNNNTGC	2 cut(s)	1327, 1361
BciT130I	CCWGG	2 cut(s)	509, 1222
BcnI	CCSGG	3 cut(s)	374, 898, 1261
BcoDI	GTCTC	6 cut(s)	383, 468, 776, 803, 978, 1313
BfaI	CTAG	3 cut(s)	20, 108, 1506
BfoI	RGCGCY	1 cut(s)	764
BisI	GCNGC	9 cut(s)	112, 434, 523, 788, 846, 849, 1051, 1257, 1313
BlsI	GCNGC	9 cut(s)	113, 435, 524, 789, 847, 850, 1052, 1258, 1314
Bme1390I	CCNGG	5 cut(s)	374, 509, 898, 1222, 1261
Bme18I	GGWCC	3 cut(s)	639, 1224, 1486
BmeT110I	CYCGRG	2 cut(s)	755, 1247
BmgBI	CACGTC	1 cut(s)	582
BmgT120I	GGNCC	6 cut(s)	369, 370, 639, 1224, 1254, 1486
BmiI	GGNNCC	2 cut(s)	371, 372
BmrFI	CCNGG	5 cut(s)	374, 509, 898, 1222, 1261
BmsI	GCATC	1 cut(s)	435
BpiI	GAAGAC	3 cut(s)	219, 219, 584
BpmI	CTGGAG	2 cut(s)	100, 491
BpuEI	CTTGAG	3 cut(s)	599, 1102, 1195
BpuMI	CCSGG	3 cut(s)	374, 898, 1261
BsaAI	YACGTR	1 cut(s)	655
BsaBI	GATNNNNATC	1 cut(s)	42
BsaHI	GRCGYC	1 cut(s)	785
BsaI	GGTCTC	2 cut(s)	383, 1313
BsaJI	CCNNGG	5 cut(s)	372, 570, 635, 996, 1088
BsaWI	WCCGGW	1 cut(s)	536
BsaXI	ACNNNNNCTCC	2 cut(s)	566, 596
Bsc4I	CCNNNNNNNGG	5 cut(s)	15, 904, 1332, 1413, 1495
Bse118I	RCCGGY	1 cut(s)	1492
Bse3DI	GCAATG	2 cut(s)	670, 1443
Bse8I	GATNNNNATC	1 cut(s)	42
BseBI	CCWGG	2 cut(s)	509, 1222
BseDI	CCNNGG	5 cut(s)	372, 570, 635, 996, 1088
BseGI	GGATG	3 cut(s)	211, 450, 888
BseJI	GATNNNNATC	1 cut(s)	42
BseLI	CCNNNNNNNGG	5 cut(s)	15, 904, 1332, 1413, 1495
BseMI	GCAATG	2 cut(s)	670, 1443
BseMII	CTCAG	1 cut(s)	145
BseRI	GAGGAG	4 cut(s)	132, 211, 371, 1429
BseSI	GKGCMC	2 cut(s)	373, 1451
BseXI	GCAGC	3 cut(s)	123, 445, 857
BseYI	CCCAGC	1 cut(s)	310
BsgI	GTGCAG	1 cut(s)	718
Bsh1236I	CGCG	8 cut(s)	407, 426, 671, 851, 878, 1053, 1317, 1404
BshFI	GGCC	8 cut(s)	371, 745, 767, 995, 1087, 1256, 1286, 1519
BsiHKAI	GWGCWC	4 cut(s)	354, 459, 615, 1451
BsiHKCI	CYCGRG	2 cut(s)	755, 1247
BsiSI	CCGG	6 cut(s)	374, 537, 898, 1260, 1464, 1493
BsiWI	CGTACG	2 cut(s)	468, 1399
BslI	CCNNNNNNNGG	5 cut(s)	15, 904, 1332, 1413, 1495
BsmAI	GTCTC	6 cut(s)	383, 468, 776, 803, 978, 1313
BsmBI	CGTCTC	3 cut(s)	776, 803, 978
BsmI	GAATGC	1 cut(s)	191
BsnI	GGCC	8 cut(s)	371, 745, 767, 995, 1087, 1256, 1286, 1519
Bso31I	GGTCTC	2 cut(s)	383, 1313
BsoBI	CYCGRG	2 cut(s)	755, 1247
Bsp120I	GGGCCC	1 cut(s)	369
Bsp1286I	GDGCHC	5 cut(s)	354, 373, 459, 615, 1451
Bsp143I	GATC	5 cut(s)	119, 672, 946, 1092, 1120
BspANI	GGCC	8 cut(s)	371, 745, 767, 995, 1087, 1256, 1286, 1519
BspCNI	CTCAG	1 cut(s)	144
BspFNI	CGCG	8 cut(s)	407, 426, 671, 851, 878, 1053, 1317, 1404
BspLI	GGNNCC	2 cut(s)	371, 372
BspPI	GGATC	2 cut(s)	114, 1100
BspTNI	GGTCTC	2 cut(s)	383, 1313
BsrBI	CCGCTC	4 cut(s)	1237, 1252, 1268, 1312
BsrDI	GCAATG	2 cut(s)	670, 1443
BsrFI	RCCGGY	1 cut(s)	1492
BssAI	RCCGGY	1 cut(s)	1492
BssECI	CCNNGG	5 cut(s)	372, 570, 635, 996, 1088
BssMI	GATC	5 cut(s)	119, 672, 946, 1092, 1120
BssNI	GRCGYC	1 cut(s)	785
BssT1I	CCWWGG	3 cut(s)	635, 996, 1088
Bst2UI	CCWGG	2 cut(s)	509, 1222
Bst4CI	ACNGT	5 cut(s)	545, 610, 808, 833, 1446
Bst6I	CTCTTC	1 cut(s)	1107
BstACI	GRCGYC	1 cut(s)	785
BstBAI	YACGTR	1 cut(s)	655
BstC8I	GCNNGC	6 cut(s)	661, 765, 911, 1254, 1266, 1288
BstDEI	CTNAG	1 cut(s)	131
BstEII	GGTNACC	1 cut(s)	539
BstENI	CCTNNNNNAGG	2 cut(s)	13, 1411
BstF5I	GGATG	3 cut(s)	211, 450, 888
BstFNI	CGCG	8 cut(s)	407, 426, 671, 851, 878, 1053, 1317, 1404
BstH2I	RGCGCY	1 cut(s)	764
BstHHI	GCGC	8 cut(s)	671, 763, 845, 853, 878, 1292, 1317, 1406
BstKTI	GATC	5 cut(s)	122, 675, 949, 1095, 1123
BstMAI	GTCTC	6 cut(s)	383, 468, 776, 803, 978, 1313
BstMBI	GATC	5 cut(s)	119, 672, 946, 1092, 1120
BstMWI	GCNNNNNNNGC	6 cut(s)	454, 751, 760, 793, 821, 1265
BstNI	CCWGG	2 cut(s)	509, 1222
BstPI	GGTNACC	1 cut(s)	539
BstSCI	CCNGG	5 cut(s)	372, 507, 896, 1220, 1259
BstSLI	GKGCMC	2 cut(s)	373, 1451
BstUI	CGCG	8 cut(s)	407, 426, 671, 851, 878, 1053, 1317, 1404
BstV1I	GCAGC	3 cut(s)	123, 445, 857
BstV2I	GAAGAC	3 cut(s)	219, 219, 584
BstX2I	RGATCY	1 cut(s)	119
BstXI	CCANNNNNNTGG	2 cut(s)	811, 941
BstYI	RGATCY	1 cut(s)	119
BsuRI	GGCC	8 cut(s)	371, 745, 767, 995, 1087, 1256, 1286, 1519
BtgZI	GCGATG	3 cut(s)	388, 1385, 1444
BtrI	CACGTC	1 cut(s)	582
BtsCI	GGATG	3 cut(s)	211, 450, 888
BtsIMutI	CAGTG	2 cut(s)	804, 1451
Cac8I	GCNNGC	6 cut(s)	661, 765, 911, 1254, 1266, 1288
CfoI	GCGC	8 cut(s)	671, 763, 845, 853, 878, 1292, 1317, 1406
Cfr10I	RCCGGY	1 cut(s)	1492
Cfr13I	GGNCC	6 cut(s)	369, 370, 639, 1224, 1254, 1486
CseI	GACGC	4 cut(s)	415, 785, 793, 1000
Csp6I	GTAC	2 cut(s)	469, 1400
CviAII	CATG	6 cut(s)	202, 439, 526, 676, 1157, 1349
CviQI	GTAC	2 cut(s)	469, 1400
DdeI	CTNAG	1 cut(s)	131
DpnI	GATC	5 cut(s)	121, 674, 948, 1094, 1122
DpnII	GATC	5 cut(s)	119, 672, 946, 1092, 1120
EaeI	YGGCCR	2 cut(s)	743, 765
Eam1104I	CTCTTC	1 cut(s)	1107
EarI	CTCTTC	1 cut(s)	1107
EciI	GGCGGA	1 cut(s)	556
Ecl136II	GAGCTC	2 cut(s)	352, 457
Eco130I	CCWWGG	3 cut(s)	635, 996, 1088
Eco147I	AGGCCT	2 cut(s)	995, 1519
Eco24I	GRGCYC	3 cut(s)	354, 373, 459
Eco31I	GGTCTC	2 cut(s)	383, 1313
Eco32I	GATATC	1 cut(s)	979
Eco47I	GGWCC	3 cut(s)	639, 1224, 1486
Eco53kI	GAGCTC	2 cut(s)	352, 457
Eco72I	CACGTG	1 cut(s)	655
Eco88I	CYCGRG	2 cut(s)	755, 1247
Eco91I	GGTNACC	1 cut(s)	539
EcoICRI	GAGCTC	2 cut(s)	352, 457
EcoNI	CCTNNNNNAGG	2 cut(s)	13, 1411
EcoO109I	RGGNCCY	1 cut(s)	370
EcoO65I	GGTNACC	1 cut(s)	539
EcoRII	CCWGG	2 cut(s)	507, 1220
EcoRV	GATATC	1 cut(s)	979
EcoT14I	CCWWGG	3 cut(s)	635, 996, 1088
EcoT22I	ATGCAT	1 cut(s)	1350
EcoT38I	GRGCYC	3 cut(s)	354, 373, 459
ErhI	CCWWGG	3 cut(s)	635, 996, 1088
Esp3I	CGTCTC	3 cut(s)	776, 803, 978
FaeI	CATG	6 cut(s)	205, 442, 529, 679, 1160, 1352
FaiI	YATR	9 cut(s)	203, 440, 527, 677, 710, 1158, 1346, 1350, 1422
FatI	CATG	6 cut(s)	201, 438, 525, 675, 1156, 1348
FauI	CCCGC	3 cut(s)	431, 1245, 1348
Fnu4HI	GCNGC	9 cut(s)	112, 434, 523, 788, 846, 849, 1051, 1257, 1313
FokI	GGATG	3 cut(s)	218, 457, 895
FriOI	GRGCYC	3 cut(s)	354, 373, 459
Fsp4HI	GCNGC	9 cut(s)	112, 434, 523, 788, 846, 849, 1051, 1257, 1313
FspBI	CTAG	3 cut(s)	20, 108, 1506
FspI	TGCGCA	1 cut(s)	1291
GlaI	GCGC	8 cut(s)	670, 762, 844, 852, 877, 1291, 1316, 1405
GluI	GCNGC	9 cut(s)	112, 434, 523, 788, 846, 849, 1051, 1257, 1313
GsaI	CCCAGC	1 cut(s)	314
GsuI	CTGGAG	2 cut(s)	100, 491
HaeII	RGCGCY	1 cut(s)	764
HaeIII	GGCC	8 cut(s)	371, 745, 767, 995, 1087, 1256, 1286, 1519
HapII	CCGG	6 cut(s)	374, 537, 898, 1260, 1464, 1493
HgaI	GACGC	4 cut(s)	415, 785, 793, 1000
HhaI	GCGC	8 cut(s)	671, 763, 845, 853, 878, 1292, 1317, 1406
Hin1I	GRCGYC	1 cut(s)	785
Hin1II	CATG	6 cut(s)	205, 442, 529, 679, 1160, 1352
Hin6I	GCGC	8 cut(s)	669, 761, 843, 851, 876, 1290, 1315, 1404
HinP1I	GCGC	8 cut(s)	669, 761, 843, 851, 876, 1290, 1315, 1404
HincII	GTYRAC	2 cut(s)	343, 1138
HindII	GTYRAC	2 cut(s)	343, 1138
HinfI	GANTC	5 cut(s)	533, 593, 748, 1301, 1454
HpaII	CCGG	6 cut(s)	374, 537, 898, 1260, 1464, 1493
HphI	GGTGA	6 cut(s)	46, 137, 286, 551, 704, 793
Hpy166II	GTNNAC	5 cut(s)	343, 597, 1138, 1192, 1449
Hpy188I	TCNGA	9 cut(s)	37, 574, 650, 955, 970, 983, 1043, 1077, 1300
Hpy188III	TCNNGA	4 cut(s)	117, 623, 1124, 1166
Hpy8I	GTNNAC	5 cut(s)	343, 597, 1138, 1192, 1449
Hpy99I	CGWCG	5 cut(s)	173, 176, 833, 1016, 1130
HpyAV	CCTTC	7 cut(s)	136, 334, 598, 859, 1305, 1421, 1477
HpyCH4III	ACNGT	5 cut(s)	545, 610, 808, 833, 1446
HpyCH4IV	ACGT	5 cut(s)	174, 581, 654, 1128, 1398
HpyCH4V	TGCA	4 cut(s)	663, 735, 1348, 1449
HpyF10VI	GCNNNNNNNGC	6 cut(s)	454, 751, 760, 793, 821, 1265
HpyF3I	CTNAG	1 cut(s)	131
HpySE526I	ACGT	5 cut(s)	174, 581, 654, 1128, 1398
Hsp92I	GRCGYC	1 cut(s)	785
Hsp92II	CATG	6 cut(s)	205, 442, 529, 679, 1160, 1352
HspAI	GCGC	8 cut(s)	669, 761, 843, 851, 876, 1290, 1315, 1404
Kzo9I	GATC	5 cut(s)	119, 672, 946, 1092, 1120
LmnI	GCTCC	4 cut(s)	119, 425, 1077, 1309
Lsp1109I	GCAGC	3 cut(s)	123, 445, 857
LweI	GCATC	1 cut(s)	435
MaeI	CTAG	3 cut(s)	20, 108, 1506
MaeII	ACGT	5 cut(s)	174, 581, 654, 1128, 1398
MaeIII	GTNAC	2 cut(s)	134, 539
MalI	GATC	5 cut(s)	121, 674, 948, 1094, 1122
MbiI	CCGCTC	4 cut(s)	1237, 1252, 1268, 1312
MboI	GATC	5 cut(s)	119, 672, 946, 1092, 1120
MboII	GAAGA	7 cut(s)	194, 219, 221, 222, 224, 589, 1124
MflI	RGATCY	1 cut(s)	119
MhlI	GDGCHC	5 cut(s)	354, 373, 459, 615, 1451
MlsI	TGGCCA	1 cut(s)	767
MluCI	AATT	5 cut(s)	151, 870, 920, 961, 971
MluNI	TGGCCA	1 cut(s)	767
MlyI	GAGTC	4 cut(s)	527, 602, 742, 1310
MmeI	TCCRAC	3 cut(s)	157, 381, 1111
Mox20I	TGGCCA	1 cut(s)	767
Mph1103I	ATGCAT	1 cut(s)	1350
MscI	TGGCCA	1 cut(s)	767
MslI	CAYNNNNRTG	2 cut(s)	443, 809
Msp20I	TGGCCA	1 cut(s)	767
MspI	CCGG	6 cut(s)	374, 537, 898, 1260, 1464, 1493
MspR9I	CCNGG	5 cut(s)	374, 509, 898, 1222, 1261
Mva1269I	GAATGC	1 cut(s)	191
MvaI	CCWGG	2 cut(s)	509, 1222
MvnI	CGCG	8 cut(s)	407, 426, 671, 851, 878, 1053, 1317, 1404
MwoI	GCNNNNNNNGC	6 cut(s)	454, 751, 760, 793, 821, 1265
NciI	CCSGG	3 cut(s)	374, 898, 1261
NdeII	GATC	5 cut(s)	119, 672, 946, 1092, 1120
NlaIII	CATG	6 cut(s)	205, 442, 529, 679, 1160, 1352
NlaIV	GGNNCC	2 cut(s)	371, 372
NmeAIII	GCCGAG	1 cut(s)	438
NmuCI	GTSAC	1 cut(s)	539
NsbI	TGCGCA	1 cut(s)	1291
NsiI	ATGCAT	1 cut(s)	1350
OliI	CACNNNNGTG	1 cut(s)	809
PaeR7I	CTCGAG	2 cut(s)	755, 1247
PceI	AGGCCT	2 cut(s)	995, 1519
PcsI	WCGNNNNNNNCGW	3 cut(s)	345, 477, 578
PctI	GAATGC	1 cut(s)	191
PfeI	GAWTC	1 cut(s)	1454
Pfl23II	CGTACG	2 cut(s)	468, 1399
PflMI	CCANNNNNTGG	2 cut(s)	1332, 1495
PfoI	TCCNGGA	1 cut(s)	1220
PkrI	GCNGC	9 cut(s)	113, 435, 524, 789, 847, 850, 1052, 1258, 1314
PleI	GAGTC	4 cut(s)	527, 601, 742, 1309
PmaCI	CACGTG	1 cut(s)	655
PmlI	CACGTG	1 cut(s)	655
PpsI	GAGTC	4 cut(s)	527, 601, 742, 1309
Ppu21I	YACGTR	1 cut(s)	655
Psp124BI	GAGCTC	2 cut(s)	354, 459
Psp6I	CCWGG	2 cut(s)	507, 1220
PspCI	CACGTG	1 cut(s)	655
PspEI	GGTNACC	1 cut(s)	539
PspFI	CCCAGC	1 cut(s)	310
PspGI	CCWGG	2 cut(s)	507, 1220
PspLI	CGTACG	2 cut(s)	468, 1399
PspN4I	GGNNCC	2 cut(s)	371, 372
PspOMI	GGGCCC	1 cut(s)	369
PspPI	GGNCC	6 cut(s)	369, 370, 639, 1224, 1254, 1486
PspXI	VCTCGAGB	2 cut(s)	755, 1247
PsuI	RGATCY	1 cut(s)	119
RsaI	GTAC	2 cut(s)	470, 1401
RsaNI	GTAC	2 cut(s)	469, 1400
RseI	CAYNNNNRTG	2 cut(s)	443, 809
SacI	GAGCTC	2 cut(s)	354, 459
SatI	GCNGC	9 cut(s)	112, 434, 523, 788, 846, 849, 1051, 1257, 1313
Sau3AI	GATC	5 cut(s)	119, 672, 946, 1092, 1120
Sau96I	GGNCC	6 cut(s)	369, 370, 639, 1224, 1254, 1486
SchI	GAGTC	4 cut(s)	527, 602, 742, 1310
ScrFI	CCNGG	5 cut(s)	374, 509, 898, 1222, 1261
SduI	GDGCHC	5 cut(s)	354, 373, 459, 615, 1451
SfaNI	GCATC	1 cut(s)	435
Sfr274I	CTCGAG	2 cut(s)	755, 1247
SinI	GGWCC	3 cut(s)	639, 1224, 1486
SlaI	CTCGAG	2 cut(s)	755, 1247
SmiMI	CAYNNNNRTG	2 cut(s)	443, 809
SmlI	CTYRAG	5 cut(s)	614, 755, 1081, 1174, 1247
SmoI	CTYRAG	5 cut(s)	614, 755, 1081, 1174, 1247
Sse9I	AATT	5 cut(s)	151, 870, 920, 961, 971
SseBI	AGGCCT	2 cut(s)	995, 1519
SspI	AATATT	1 cut(s)	1470
SspMI	CTAG	3 cut(s)	20, 108, 1506
SstI	GAGCTC	2 cut(s)	354, 459
StuI	AGGCCT	2 cut(s)	995, 1519
StyD4I	CCNGG	5 cut(s)	372, 507, 896, 1220, 1259
StyI	CCWWGG	3 cut(s)	635, 996, 1088
TaaI	ACNGT	5 cut(s)	545, 610, 808, 833, 1446
TaiI	ACGT	5 cut(s)	177, 584, 657, 1131, 1401
TaqII	GACCGA	1 cut(s)	545
TasI	AATT	5 cut(s)	151, 870, 920, 961, 971
TauI	GCSGC	6 cut(s)	525, 790, 851, 1053, 1259, 1315
TfiI	GAWTC	1 cut(s)	1454
TscAI	CASTG	2 cut(s)	811, 1451
TseFI	GTSAC	1 cut(s)	539
TseI	GCWGC	3 cut(s)	111, 433, 845
Tsp45I	GTSAC	1 cut(s)	539
TspDTI	ATGAA	4 cut(s)	190, 222, 878, 1145
TspGWI	ACGGA	2 cut(s)	456, 486
TspRI	CASTG	2 cut(s)	811, 1451
Van91I	CCANNNNNTGG	2 cut(s)	1332, 1495
VneI	GTGCAC	1 cut(s)	1447
VpaK11BI	GGWCC	3 cut(s)	639, 1224, 1486
XagI	CCTNNNNNAGG	2 cut(s)	13, 1411
XapI	RAATTY	2 cut(s)	920, 961
XhoI	CTCGAG	2 cut(s)	755, 1247
XspI	CTAG	3 cut(s)	20, 108, 1506
Zsp2I	ATGCAT	1 cut(s)	1350

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.