Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0014436

Physical Location & Seq

Genomic Coordinates Forward (+)

19380547 .. 19382073

Gene Structure 1527 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG206400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1527 bp

ATGGATACCTTGTTTAGGCTAGTCACTCTTCAACAACAACAACAACAACAGCAACAACAATCTGATCAATCTTTCAATTCATCCACCAGAACCACCACCACCACTTCCAGCAGCTCCAGATCCTTCACTCACCACCCACAACACCACCAACACTACCCCCAACAAAACGACGTTGAAGAATGCTTCAACTTTTTCATGGATGAAGAAGACTTCTCCTCTTCTTCTTCTAAGGCTCACTACTATCCCACCCCACCAACCCCTACCCCGCCTCCGCCGCTCACCACCACGACGACCACTCCCACCCCCACCGATTTCTCCTTCTCCCCCGCCCACGACCTCCTCAACTTCGAATTCTCCTCCCCCAAGTGGGCCCCTGACCTCCTCTTAGAAACCGCCCGGGCCATTGCTGACAAGAACAGCGCAAGAGTTCAACAGCTCATGTGGATGCTCAACGAGCTCGGCTCTCCCTACGGCGACACCGATCAAAAACTCACCTCCTACTTCCTCCAGGCCCTCTTCAGCCGCATGACCGACTCCGGCGACCGCTGCTACCGGACCCTAGTCTCGGCCTCGGAGAAAACCTGCTCTTTCGAATCCACCAGGAAAATGGTCTTGAAATTTCAGGAGGTGAGTCCGTGGACTACATTCGGTCACGTAGCTTGTAACGGTGCAATTATGGAAGCCTTTGACGGTGAGGCCAAGCTTCACATAGTCGACATCAGCAACACTTACTGCACGCAGTGGCCGACTCTGCTGGAGGCTCTCGCCACCCGAACTGATGAGACGCCGCATCTGCGTCTCACCAGTATAGTCGTCAGCAGAGCTGGAGATGGCTCTGCTGGCGTTGGCGCCACGCAAAAAGTAATGAAGGAAATCGGCGCCAGAATGGAGAAATTCGCGCGCTTAATGGGCGTGCCGTTCAAATTCAATGCGATCCAGCACTCCGGCGACCTCGCGGAGCTGAACTTGGCTGATTTGGATGTCAGGGACGATGAGGCCTTGGCCATCAACTGTGTTGGATCGTTACATTCAATCGTGGCCGTTGGAAACCGCCGTGATTATTTGATATCGGCTTTTCGGAGCTTAAAGCCGAGGATCATTACTGTTGTGGAGGAAGAAGCTGACCTAGACGTTGGAGTTGACGGGTTTGACTTCGTCCAAGGGTTTCAGGAATGCTTGAGGTGGTTTAGGGTTTACTTTGAGGCTCTGGACGAGAGCTTCGCCCGGACGAGTAACGAGAGGTTGATGCTCGAGAGGGCGGCCGGCCGAGCCATCGTTGACTTGGTGGCTTGTGCGCCGCCGGACTCCGTCGAGCGGCGCGAGTCGGCCACGCGCTGGTCGAGGCGTATGCATGGGGCCGGGTTCAGTCCAACTTCATTTAGTGATGAGGTGTGCGATGACGTACGGGCCTTGTTGCGGAGGTACAAGGAAGGTTGGTCAATGACGCAGTGTGGTGACTCCGTCGCCGGAATATTCTTGTCGTGGAAGGACCAGCCGGTGGTTTGGGCCAGCGCTTGGAGGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008356 GO:0009889 GO:0009956 GO:0009987 GO:0010468 GO:0010556 GO:0010817 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032350 GO:0032501 GO:0032502 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045786 GO:0045930 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051171 GO:0051252 GO:0051301 GO:0051726 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060255 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098771 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

508

Amino Acids

57.14

Weight (kDa)

5.32

Isoelectric Point (pI)

48.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	126 - 507	1.4e-103	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014436)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37650
fragaria_vesca	FvH4_1g17690
malus_domestica	MD02G1185900.v1.1 MD15G1307800.v1.1
prunus_persica	Prupe.6G220800_v2.0.a1
pyrus_communis	pycom02g15090 pycom15g26030
rosa_chinensis	RchiOBHm_Chr2g0108031
rosa_laevigata	RLG00000017634
rosa_multiflora	Rmu_sc0001484.1_g000021
rosa_roxburghii	Rroxscaffold_2G00135710
rosa_rugosa	Rorug02G0149800
rosa_samantha	Rh2AG198800 Rh2BG211700 Rh2CG203400 Rh2DG206400
rosa_wichuraiana	Rw2G015780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	590
AccB1I	GGYRCC	2 cut(s)	848, 878
AccB7I	CCANNNNNTGG	3 cut(s)	1335, 1498, 1515
AccBSI	CCGCTC	2 cut(s)	277, 1315
AccI	GTMKAC	1 cut(s)	714
AccII	CGCG	5 cut(s)	899, 901, 956, 1320, 1333
AclWI	GGATC	4 cut(s)	114, 928, 1027, 1103
AcoI	YGGCCR	6 cut(s)	743, 1002, 1038, 1260, 1264, 1326
AcsI	RAATTY	4 cut(s)	350, 617, 893, 923
AcuI	CTGAAG	1 cut(s)	502
AcyI	GRCGYC	3 cut(s)	785, 849, 879
AdeI	CACNNNGTG	1 cut(s)	741
AfaI	GTAC	2 cut(s)	1404, 1424
AfeI	AGCGCT	1 cut(s)	1513
AfiI	CCNNNNNNNGG	9 cut(s)	15, 366, 367, 565, 1314, 1335, 1416, 1498, 1515
AgsI	TTSAA	9 cut(s)	32, 76, 176, 187, 431, 616, 922, 928, 1032
AjnI	CCWGG	2 cut(s)	507, 599
Alw21I	GWGCWC	1 cut(s)	459
Alw26I	GTCTC	3 cut(s)	568, 776, 803
AlwI	GGATC	4 cut(s)	114, 928, 1027, 1103
Ama87I	CYCGRG	2 cut(s)	396, 1250
Aor51HI	AGCGCT	1 cut(s)	1513
ApaI	GGGCCC	1 cut(s)	373
ApeKI	GCWGC	2 cut(s)	111, 546
ApoI	RAATTY	4 cut(s)	350, 617, 893, 923
ArsI	GACNNNNNNTTYG	2 cut(s)	1202, 1234
AspLEI	GCGC	9 cut(s)	422, 851, 881, 901, 903, 1297, 1320, 1335, 1514
AspS9I	GGNCC	9 cut(s)	369, 370, 399, 511, 555, 1356, 1407, 1489, 1506
AsuC2I	CCSGG	4 cut(s)	397, 398, 1225, 1360
AsuHPI	GGTGA	7 cut(s)	122, 271, 484, 640, 704, 793, 1466
AsuII	TTCGAA	2 cut(s)	348, 591
AvaI	CYCGRG	2 cut(s)	396, 1250
AvaII	GGWCC	2 cut(s)	555, 1489
BaeGI	GKGCMC	1 cut(s)	373
BalI	TGGCCA	1 cut(s)	1004
BanI	GGYRCC	2 cut(s)	848, 878
BanII	GRGCYC	2 cut(s)	373, 459
BbsI	GAAGAC	1 cut(s)	213
Bbv12I	GWGCWC	1 cut(s)	459
BbvI	GCAGC	2 cut(s)	123, 533
BccI	CCATC	3 cut(s)	824, 1013, 1280
BceAI	ACGGC	4 cut(s)	487, 901, 1025, 1038
BcgI	CGANNNNNNTGC	2 cut(s)	1330, 1364
BciT130I	CCWGG	2 cut(s)	509, 601
BclI	TGATCA	1 cut(s)	64
BcnI	CCSGG	4 cut(s)	397, 398, 1225, 1360
BcoDI	GTCTC	3 cut(s)	568, 776, 803
BfaI	CTAG	3 cut(s)	20, 560, 1127
BfoI	RGCGCY	3 cut(s)	852, 882, 1515
BfuAI	ACCTGC	1 cut(s)	590
BisI	GCNGC	8 cut(s)	112, 275, 523, 547, 788, 1260, 1298, 1316
BlsI	GCNGC	8 cut(s)	113, 276, 524, 548, 789, 1261, 1299, 1317
Bme1390I	CCNGG	6 cut(s)	397, 398, 509, 601, 1225, 1360
Bme18I	GGWCC	2 cut(s)	555, 1489
BmeT110I	CYCGRG	2 cut(s)	396, 1250
BmgT120I	GGNCC	9 cut(s)	369, 370, 399, 511, 555, 1356, 1407, 1489, 1506
BmiI	GGNNCC	6 cut(s)	371, 372, 557, 850, 880, 1357
BmrFI	CCNGG	6 cut(s)	397, 398, 509, 601, 1225, 1360
BmsI	GCATC	3 cut(s)	435, 799, 1236
BoxI	GACNNNNGTC	1 cut(s)	560
BpiI	GAAGAC	1 cut(s)	213
BplI	GAGNNNNNCTC	2 cut(s)	446, 478
BpmI	CTGGAG	4 cut(s)	100, 491, 776, 846
Bpu14I	TTCGAA	2 cut(s)	348, 591
BpuEI	CTTGAG	1 cut(s)	1198
BpuMI	CCSGG	4 cut(s)	397, 398, 1225, 1360
BsaAI	YACGTR	1 cut(s)	655
BsaHI	GRCGYC	3 cut(s)	785, 849, 879
BsaJI	CCNNGG	6 cut(s)	396, 570, 635, 999, 1091, 1159
BsaWI	WCCGGW	1 cut(s)	552
BsaXI	ACNNNNNCTCC	4 cut(s)	253, 280, 283, 310
Bsc4I	CCNNNNNNNGG	9 cut(s)	15, 366, 367, 565, 1314, 1335, 1416, 1498, 1515
Bse118I	RCCGGY	2 cut(s)	1262, 1495
Bse1I	ACTGG	1 cut(s)	804
Bse3DI	GCAATG	1 cut(s)	402
BseBI	CCWGG	2 cut(s)	509, 601
BseDI	CCNNGG	6 cut(s)	396, 570, 635, 999, 1091, 1159
BseGI	GGATG	4 cut(s)	80, 205, 450, 985
BseLI	CCNNNNNNNGG	9 cut(s)	15, 366, 367, 565, 1314, 1335, 1416, 1498, 1515
BseMI	GCAATG	1 cut(s)	402
BseNI	ACTGG	1 cut(s)	804
BsePI	GCGCGC	1 cut(s)	899
BseRI	GAGGAG	4 cut(s)	205, 329, 346, 371
BseSI	GKGCMC	1 cut(s)	373
BseX3I	CGGCCG	2 cut(s)	1260, 1264
BseXI	GCAGC	2 cut(s)	123, 533
BsgI	GTGCAG	1 cut(s)	718
Bsh1236I	CGCG	5 cut(s)	899, 901, 956, 1320, 1333
Bsh1285I	CGRYCG	3 cut(s)	544, 1263, 1267
BshNI	GGYRCC	2 cut(s)	848, 878
BsiEI	CGRYCG	3 cut(s)	544, 1263, 1267
BsiHKAI	GWGCWC	1 cut(s)	459
BsiHKCI	CYCGRG	2 cut(s)	396, 1250
BsiWI	CGTACG	1 cut(s)	1402
BslFI	GGGAC	1 cut(s)	1001
BslI	CCNNNNNNNGG	9 cut(s)	15, 366, 367, 565, 1314, 1335, 1416, 1498, 1515
BsmAI	GTCTC	3 cut(s)	568, 776, 803
BsmBI	CGTCTC	2 cut(s)	776, 803
BsmFI	GGGAC	1 cut(s)	1001
BsmI	GAATGC	2 cut(s)	185, 1178
BsoBI	CYCGRG	2 cut(s)	396, 1250
Bsp119I	TTCGAA	2 cut(s)	348, 591
Bsp120I	GGGCCC	1 cut(s)	369
Bsp1286I	GDGCHC	2 cut(s)	373, 459
Bsp143I	GATC	6 cut(s)	64, 119, 481, 933, 1019, 1095
BspFNI	CGCG	5 cut(s)	899, 901, 956, 1320, 1333
BspLI	GGNNCC	6 cut(s)	371, 372, 557, 850, 880, 1357
BspMI	ACCTGC	1 cut(s)	590
BspPI	GGATC	4 cut(s)	114, 928, 1027, 1103
BspT104I	TTCGAA	2 cut(s)	348, 591
BspT107I	GGYRCC	2 cut(s)	848, 878
BsrBI	CCGCTC	2 cut(s)	277, 1315
BsrDI	GCAATG	1 cut(s)	402
BsrFI	RCCGGY	2 cut(s)	1262, 1495
BsrI	ACTGG	1 cut(s)	804
BssAI	RCCGGY	2 cut(s)	1262, 1495
BssECI	CCNNGG	6 cut(s)	396, 570, 635, 999, 1091, 1159
BssHII	GCGCGC	1 cut(s)	899
BssMI	GATC	6 cut(s)	64, 119, 481, 933, 1019, 1095
BssNI	GRCGYC	3 cut(s)	785, 849, 879
BssT1I	CCWWGG	2 cut(s)	999, 1159
Bst2UI	CCWGG	2 cut(s)	509, 601
Bst4CI	ACNGT	4 cut(s)	668, 692, 1013, 1105
Bst6I	CTCTTC	3 cut(s)	33, 223, 521
BstACI	GRCGYC	3 cut(s)	785, 849, 879
BstBAI	YACGTR	1 cut(s)	655
BstBI	TTCGAA	2 cut(s)	348, 591
BstC8I	GCNNGC	6 cut(s)	737, 841, 901, 914, 1264, 1510
BstDEI	CTNAG	2 cut(s)	228, 385
BstDSI	CCRYGG	1 cut(s)	635
BstENI	CCTNNNNNAGG	1 cut(s)	13
BstF5I	GGATG	4 cut(s)	80, 205, 450, 985
BstFNI	CGCG	5 cut(s)	899, 901, 956, 1320, 1333
BstH2I	RGCGCY	3 cut(s)	852, 882, 1515
BstHHI	GCGC	9 cut(s)	422, 851, 881, 901, 903, 1297, 1320, 1335, 1514
BstKTI	GATC	6 cut(s)	67, 122, 484, 936, 1022, 1098
BstMAI	GTCTC	3 cut(s)	568, 776, 803
BstMBI	GATC	6 cut(s)	64, 119, 481, 933, 1019, 1095
BstMCI	CGRYCG	3 cut(s)	544, 1263, 1267
BstMWI	GCNNNNNNNGC	8 cut(s)	274, 454, 546, 751, 793, 840, 909, 1268
BstNI	CCWGG	2 cut(s)	509, 601
BstPAI	GACNNNNGTC	1 cut(s)	560
BstSCI	CCNGG	6 cut(s)	395, 396, 507, 599, 1223, 1358
BstSLI	GKGCMC	1 cut(s)	373
BstUI	CGCG	5 cut(s)	899, 901, 956, 1320, 1333
BstV1I	GCAGC	2 cut(s)	123, 533
BstV2I	GAAGAC	1 cut(s)	213
BstX2I	RGATCY	1 cut(s)	119
BstXI	CCANNNNNNTGG	1 cut(s)	607
BstYI	RGATCY	1 cut(s)	119
BstZI	CGGCCG	2 cut(s)	1260, 1264
BtgI	CCRYGG	1 cut(s)	635
BtgZI	GCGATG	1 cut(s)	1410
BtsCI	GGATG	4 cut(s)	80, 205, 450, 985
BtsI	GCAGTG	2 cut(s)	746, 1454
BtsIMutI	CAGTG	2 cut(s)	746, 1454
BveI	ACCTGC	1 cut(s)	590
Cac8I	GCNNGC	6 cut(s)	737, 841, 901, 914, 1264, 1510
CfoI	GCGC	9 cut(s)	422, 851, 881, 901, 903, 1297, 1320, 1335, 1514
Cfr10I	RCCGGY	2 cut(s)	1262, 1495
Cfr13I	GGNCC	9 cut(s)	369, 370, 399, 511, 555, 1356, 1407, 1489, 1506
Cfr9I	CCCGGG	1 cut(s)	396
CseI	GACGC	3 cut(s)	785, 793, 1453
Csp6I	GTAC	2 cut(s)	1403, 1423
CviAII	CATG	4 cut(s)	196, 439, 526, 1352
CviQI	GTAC	2 cut(s)	1403, 1423
DdeI	CTNAG	2 cut(s)	228, 385
DinI	GGCGCC	2 cut(s)	850, 880
DpnI	GATC	6 cut(s)	66, 121, 483, 935, 1021, 1097
DpnII	GATC	6 cut(s)	64, 119, 481, 933, 1019, 1095
DraIII	CACNNNGTG	1 cut(s)	741
EaeI	YGGCCR	6 cut(s)	743, 1002, 1038, 1260, 1264, 1326
EagI	CGGCCG	2 cut(s)	1260, 1264
Eam1104I	CTCTTC	3 cut(s)	33, 223, 521
EarI	CTCTTC	3 cut(s)	33, 223, 521
EciI	GGCGGA	1 cut(s)	261
Ecl136II	GAGCTC	1 cut(s)	457
EclXI	CGGCCG	2 cut(s)	1260, 1264
Eco130I	CCWWGG	2 cut(s)	999, 1159
Eco147I	AGGCCT	2 cut(s)	998, 1522
Eco24I	GRGCYC	2 cut(s)	373, 459
Eco32I	GATATC	1 cut(s)	1068
Eco47I	GGWCC	2 cut(s)	555, 1489
Eco47III	AGCGCT	1 cut(s)	1513
Eco52I	CGGCCG	2 cut(s)	1260, 1264
Eco53kI	GAGCTC	1 cut(s)	457
Eco57I	CTGAAG	1 cut(s)	502
Eco88I	CYCGRG	2 cut(s)	396, 1250
EcoICRI	GAGCTC	1 cut(s)	457
EcoNI	CCTNNNNNAGG	1 cut(s)	13
EcoO109I	RGGNCCY	2 cut(s)	370, 511
EcoRI	GAATTC	1 cut(s)	350
EcoRII	CCWGG	2 cut(s)	507, 599
EcoRV	GATATC	1 cut(s)	1068
EcoT14I	CCWWGG	2 cut(s)	999, 1159
EcoT22I	ATGCAT	1 cut(s)	1353
EcoT38I	GRGCYC	2 cut(s)	373, 459
EgeI	GGCGCC	2 cut(s)	850, 880
EheI	GGCGCC	2 cut(s)	850, 880
ErhI	CCWWGG	2 cut(s)	999, 1159
Esp3I	CGTCTC	2 cut(s)	776, 803
FaeI	CATG	4 cut(s)	199, 442, 529, 1355
FaiI	YATR	8 cut(s)	197, 440, 527, 677, 710, 809, 1349, 1353
FaqI	GGGAC	1 cut(s)	1001
FatI	CATG	4 cut(s)	195, 438, 525, 1351
FauI	CCCGC	2 cut(s)	273, 334
FbaI	TGATCA	1 cut(s)	64
FblI	GTMKAC	1 cut(s)	714
Fnu4HI	GCNGC	8 cut(s)	112, 275, 523, 547, 788, 1260, 1298, 1316
FokI	GGATG	4 cut(s)	67, 212, 457, 992
FriOI	GRGCYC	2 cut(s)	373, 459
FseI	GGCCGGCC	1 cut(s)	1266
Fsp4HI	GCNGC	8 cut(s)	112, 275, 523, 547, 788, 1260, 1298, 1316
FspBI	CTAG	3 cut(s)	20, 560, 1127
GlaI	GCGC	9 cut(s)	421, 850, 880, 900, 902, 1296, 1319, 1334, 1513
GluI	GCNGC	8 cut(s)	112, 275, 523, 547, 788, 1260, 1298, 1316
GsuI	CTGGAG	4 cut(s)	100, 491, 776, 846
HaeII	RGCGCY	3 cut(s)	852, 882, 1515
HgaI	GACGC	3 cut(s)	785, 793, 1453
HhaI	GCGC	9 cut(s)	422, 851, 881, 901, 903, 1297, 1320, 1335, 1514
Hin1I	GRCGYC	3 cut(s)	785, 849, 879
Hin1II	CATG	4 cut(s)	199, 442, 529, 1355
Hin6I	GCGC	9 cut(s)	420, 849, 879, 899, 901, 1295, 1318, 1333, 1512
HinP1I	GCGC	9 cut(s)	420, 849, 879, 899, 901, 1295, 1318, 1333, 1512
HincII	GTYRAC	3 cut(s)	715, 1141, 1279
HindII	GTYRAC	3 cut(s)	715, 1141, 1279
HindIII	AAGCTT	1 cut(s)	701
HinfI	GANTC	7 cut(s)	533, 593, 631, 748, 1304, 1322, 1457
HphI	GGTGA	7 cut(s)	122, 271, 484, 640, 704, 793, 1466
Hpy166II	GTNNAC	5 cut(s)	639, 715, 1141, 1195, 1279
Hpy188I	TCNGA	3 cut(s)	64, 574, 1080
Hpy188III	TCNNGA	6 cut(s)	117, 613, 623, 1169, 1208, 1252
Hpy8I	GTNNAC	5 cut(s)	639, 715, 1141, 1195, 1279
Hpy99I	CGWCG	4 cut(s)	173, 292, 1313, 1466
HpyAV	CCTTC	5 cut(s)	133, 328, 862, 1424, 1480
HpyCH4III	ACNGT	4 cut(s)	668, 692, 1013, 1105
HpyCH4IV	ACGT	4 cut(s)	171, 654, 1131, 1401
HpyCH4V	TGCA	3 cut(s)	671, 735, 1351
HpyF10VI	GCNNNNNNNGC	8 cut(s)	274, 454, 546, 751, 793, 840, 909, 1268
HpyF3I	CTNAG	2 cut(s)	228, 385
HpySE526I	ACGT	4 cut(s)	171, 654, 1131, 1401
Hsp92I	GRCGYC	3 cut(s)	785, 849, 879
Hsp92II	CATG	4 cut(s)	199, 442, 529, 1355
HspAI	GCGC	9 cut(s)	420, 849, 879, 899, 901, 1295, 1318, 1333, 1512
KasI	GGCGCC	2 cut(s)	848, 878
KroI	GCCGGC	1 cut(s)	1262
KroNI	GCCGGC	1 cut(s)	1264
Ksp22I	TGATCA	1 cut(s)	64
Kzo9I	GATC	6 cut(s)	64, 119, 481, 933, 1019, 1095
LmnI	GCTCC	3 cut(s)	119, 958, 1080
Lsp1109I	GCAGC	2 cut(s)	123, 533
LweI	GCATC	3 cut(s)	435, 799, 1236
MaeI	CTAG	3 cut(s)	20, 560, 1127
MaeII	ACGT	4 cut(s)	171, 654, 1131, 1401
MaeIII	GTNAC	6 cut(s)	22, 650, 662, 1023, 1232, 1454
MalI	GATC	6 cut(s)	66, 121, 483, 935, 1021, 1097
MbiI	CCGCTC	2 cut(s)	277, 1315
MboI	GATC	6 cut(s)	64, 119, 481, 933, 1019, 1095
MboII	GAAGA	9 cut(s)	20, 188, 210, 213, 215, 216, 218, 508, 1127
MflI	RGATCY	1 cut(s)	119
MhlI	GDGCHC	2 cut(s)	373, 459
MlsI	TGGCCA	1 cut(s)	1004
MluCI	AATT	6 cut(s)	76, 350, 617, 672, 893, 923
MluNI	TGGCCA	1 cut(s)	1004
Mly113I	GGCGCC	2 cut(s)	849, 879
MlyI	GAGTC	6 cut(s)	527, 640, 742, 1298, 1331, 1451
MmeI	TCCRAC	4 cut(s)	997, 1024, 1114, 1394
Mox20I	TGGCCA	1 cut(s)	1004
Mph1103I	ATGCAT	1 cut(s)	1353
MroNI	GCCGGC	1 cut(s)	1262
MscI	TGGCCA	1 cut(s)	1004
MseI	TTAA	2 cut(s)	905, 1085
MslI	CAYNNNNRTG	1 cut(s)	443
Msp20I	TGGCCA	1 cut(s)	1004
MspA1I	CMGCKG	1 cut(s)	546
MspR9I	CCNGG	6 cut(s)	397, 398, 509, 601, 1225, 1360
Mva1269I	GAATGC	2 cut(s)	185, 1178
MvaI	CCWGG	2 cut(s)	509, 601
MvnI	CGCG	5 cut(s)	899, 901, 956, 1320, 1333
MwoI	GCNNNNNNNGC	8 cut(s)	274, 454, 546, 751, 793, 840, 909, 1268
NaeI	GCCGGC	1 cut(s)	1264
NarI	GGCGCC	2 cut(s)	849, 879
NciI	CCSGG	4 cut(s)	397, 398, 1225, 1360
NdeII	GATC	6 cut(s)	64, 119, 481, 933, 1019, 1095
NgoMIV	GCCGGC	1 cut(s)	1262
NlaIII	CATG	4 cut(s)	199, 442, 529, 1355
NlaIV	GGNNCC	6 cut(s)	371, 372, 557, 850, 880, 1357
NmeAIII	GCCGAG	4 cut(s)	438, 545, 1116, 1292
NmuCI	GTSAC	3 cut(s)	22, 650, 1454
NsiI	ATGCAT	1 cut(s)	1353
NspV	TTCGAA	2 cut(s)	348, 591
PaeR7I	CTCGAG	1 cut(s)	1250
PauI	GCGCGC	1 cut(s)	899
PceI	AGGCCT	2 cut(s)	998, 1522
PcsI	WCGNNNNNNNCGW	4 cut(s)	477, 743, 1317, 1337
PctI	GAATGC	2 cut(s)	185, 1178
PdiI	GCCGGC	1 cut(s)	1264
PfeI	GAWTC	1 cut(s)	593
Pfl23II	CGTACG	1 cut(s)	1402
PflFI	GACNNNGTC	3 cut(s)	1154, 1307, 1460
PflMI	CCANNNNNTGG	3 cut(s)	1335, 1498, 1515
PkrI	GCNGC	8 cut(s)	113, 276, 524, 548, 789, 1261, 1299, 1317
PleI	GAGTC	6 cut(s)	527, 639, 742, 1298, 1330, 1451
PluTI	GGCGCC	2 cut(s)	852, 882
PpsI	GAGTC	6 cut(s)	527, 639, 742, 1298, 1330, 1451
Ppu21I	YACGTR	1 cut(s)	655
PshAI	GACNNNNGTC	1 cut(s)	560
Psp124BI	GAGCTC	1 cut(s)	459
Psp6I	CCWGG	2 cut(s)	507, 599
PspGI	CCWGG	2 cut(s)	507, 599
PspLI	CGTACG	1 cut(s)	1402
PspN4I	GGNNCC	6 cut(s)	371, 372, 557, 850, 880, 1357
PspOMI	GGGCCC	1 cut(s)	369
PspPI	GGNCC	9 cut(s)	369, 370, 399, 511, 555, 1356, 1407, 1489, 1506
PsuI	RGATCY	1 cut(s)	119
PsyI	GACNNNGTC	3 cut(s)	1154, 1307, 1460
PteI	GCGCGC	1 cut(s)	899
RigI	GGCCGGCC	1 cut(s)	1266
RsaI	GTAC	2 cut(s)	1404, 1424
RsaNI	GTAC	2 cut(s)	1403, 1423
RseI	CAYNNNNRTG	1 cut(s)	443
SacI	GAGCTC	1 cut(s)	459
SalI	GTCGAC	1 cut(s)	713
SaqAI	TTAA	2 cut(s)	905, 1085
SatI	GCNGC	8 cut(s)	112, 275, 523, 547, 788, 1260, 1298, 1316
Sau3AI	GATC	6 cut(s)	64, 119, 481, 933, 1019, 1095
Sau96I	GGNCC	9 cut(s)	369, 370, 399, 511, 555, 1356, 1407, 1489, 1506
SchI	GAGTC	6 cut(s)	527, 640, 742, 1298, 1331, 1451
ScrFI	CCNGG	6 cut(s)	397, 398, 509, 601, 1225, 1360
SduI	GDGCHC	2 cut(s)	373, 459
SfaNI	GCATC	3 cut(s)	435, 799, 1236
SfoI	GGCGCC	2 cut(s)	850, 880
Sfr274I	CTCGAG	1 cut(s)	1250
SfuI	TTCGAA	2 cut(s)	348, 591
SinI	GGWCC	2 cut(s)	555, 1489
SlaI	CTCGAG	1 cut(s)	1250
SmaI	CCCGGG	1 cut(s)	398
SmiMI	CAYNNNNRTG	1 cut(s)	443
SmlI	CTYRAG	2 cut(s)	1177, 1250
SmoI	CTYRAG	2 cut(s)	1177, 1250
SrfI	GCCCGGGC	1 cut(s)	398
Sse9I	AATT	6 cut(s)	76, 350, 617, 672, 893, 923
SseBI	AGGCCT	2 cut(s)	998, 1522
SspDI	GGCGCC	2 cut(s)	848, 878
SspI	AATATT	1 cut(s)	1473
SspMI	CTAG	3 cut(s)	20, 560, 1127
SstI	GAGCTC	1 cut(s)	459
StuI	AGGCCT	2 cut(s)	998, 1522
StyD4I	CCNGG	6 cut(s)	395, 396, 507, 599, 1223, 1358
StyI	CCWWGG	2 cut(s)	999, 1159
TaaI	ACNGT	4 cut(s)	668, 692, 1013, 1105
TaiI	ACGT	4 cut(s)	174, 657, 1134, 1404
TaqI	TCGA	6 cut(s)	348, 591, 714, 1251, 1311, 1340
TaqII	GACCGA	2 cut(s)	545, 638
TasI	AATT	6 cut(s)	76, 350, 617, 672, 893, 923
TauI	GCSGC	6 cut(s)	277, 525, 790, 1262, 1300, 1318
TfiI	GAWTC	1 cut(s)	593
Tru1I	TTAA	2 cut(s)	905, 1085
Tru9I	TTAA	2 cut(s)	905, 1085
TscAI	CASTG	2 cut(s)	746, 1454
TseFI	GTSAC	3 cut(s)	22, 650, 1454
TseI	GCWGC	2 cut(s)	111, 546
Tsp45I	GTSAC	3 cut(s)	22, 650, 1454
TspDTI	ATGAA	5 cut(s)	69, 184, 216, 881, 1365
TspGWI	ACGGA	3 cut(s)	624, 1297, 1450
TspMI	CCCGGG	1 cut(s)	396
TspRI	CASTG	2 cut(s)	746, 1454
Tth111I	GACNNNGTC	3 cut(s)	1154, 1307, 1460
Van91I	CCANNNNNTGG	3 cut(s)	1335, 1498, 1515
VpaK11BI	GGWCC	2 cut(s)	555, 1489
XagI	CCTNNNNNAGG	1 cut(s)	13
XapI	RAATTY	4 cut(s)	350, 617, 893, 923
XcmI	CCANNNNNNNNNTGG	2 cut(s)	604, 1279
XhoI	CTCGAG	1 cut(s)	1250
XmaI	CCCGGG	1 cut(s)	396
XmiI	GTMKAC	1 cut(s)	714
XspI	CTAG	3 cut(s)	20, 560, 1127
Zsp2I	ATGCAT	1 cut(s)	1353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.