MD02G1194100.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014085
Physical Location & Seq
Reverse (-)
18479144 .. 18483873
4730 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1194100.v1.1.491

Sequence Viewer

Length: 2964 bp
ATGACACAATTAGCAGCTGTAAAGCCCGTTTTAGTATTATTTTCCGCGCTGGCATGCGTTTTAACCGTCGCTGCCTCTTTGGCCGGCGACACTCAGCACTTGATTCAGGTCAAGGCCCAACTGTCCGACCCGGACGGAAATCTCGACGACTGGGTCCCGAGCACGGATCCCAATCCATGCAACTGGACGGGAATTACATGCGACCCGAAGATCGACTCTGTTCTTGCTGTAAACTTATCCGGGCTTAACGTTTCCGGCGGGTTTCCGTTCGGGTTTTGTAGCATTCGGACCCTCCAGAACCTCTCTCTGTCCGTCAACTCCATCGGCGGCTCTCTCCAAACCCACACCCTCTCTCTCTGCTCCCACCTCCATGTTCTCGAGCTCGACAACAACCTCTTCGTCGGAACATTGCCGGAGTTTCAGCCGGAGTTCACCGACTTGCGAATCCTGAACTTCATGGACAACAACTTCACCGGCGACATTCCGGCGAGTTTCGGCCGGTTTCCCGCCTTGCAGGTCCTGATGCTTTCCGGGAACTTGCTCACCGGCACAATCCCCAAGTTCTTGGGCAATCTCAGTGAGTTGACTCACTTGGAACTCGCTATCAATCCATTGATGAAGCCCGGCGAGCTACCTTCCGAAATCGGAAACTTGACGAAGCTCCAGAACCTCTTTGCCCCCCAATGCAACCTCAGAGGCCGTATACCGGACTCCATCGGAAACCTGGCGTCTCTTACGAACCTCGATTTGTCTACGAATTCGCTATCCGGTACGATTCCGGCGAGCATCGGCGGATTGGCAAGCGTGACGCAGATCGAGCTATTCGCAAACCAGATGTTCGGCGAACTGCCCGAGAGCATAGGAAACCTGACTTTTCTCCAGTACTTAGATCTCTCTATGAACGGCTTCACCGGAAAATTCCCAGAAAAAATTGCACAATTGCAATTGGAGTCTCTGAATCTCAACGACAATTTCTTCTCCGGCGAGCTTCCTCCGATCTTGGCTTCCAATCCCAACTTGCAGCAGTTCAAAATCTTCAACAACAGCTTCTCCGGCTCCCTCCCGGAAAATCTCGGCCGATATTCCGATTTGGCAGACCTCGATGTATCGACAAACAAGTTTTCCGGCGAGCTGCCGAAGTTCCTCTGTTACCGGAAAAAGCTCTGGAATTTGATAGTTATGGAGAACCAGTTCTCCGGAAATTTACCCGATACGTTAAACAAGTGTCATTCTCTGGAATACGTTCGAATCGAATTCAATGAGCTCGACGGCGTCGTTTCGGGCATGTTTTGGGGTCTGCCTGGGCTATATTTCCTGACGATGAATAACAACAGATTCAACGGCAGCGTCTCTCCGTCGATTTCGGCGGCTACAAGATTCTCGACGCTTACGATCTCCAGCAATGAATTTTCCGGCGCACTTCCGTCGGCGATTTGTAAGCTTACGAACCTCTCGACCCTCGACGTGAGCAACAACCAATTCTCCGGCGACTTGCCGTCGTGCATGACGGAGCTGAAGATTTTGCAGAAGCTAAAAATGGAGGAGAACATGTTCTCCGGTCAGATTCCGAGTTCAGTGAGTTCGTGGACCCAGTTGACGGAGCTGAACCTATCTCACAACCACTTATCGGGTGGGATCCCACCGCAACTCGGCTACTTGCCGGTATTGAATTACTTGGATCTCTCGGAAAATTTACTCACCGGTGAGATTCCGGCGGAGTTGACCAAGCTCAGGCTCAATCAGTTCAACGTCTCGAATAATAAACTGTACGGAAAAATACCTACGGGTTTCGATTACAGTCCCTTCAGCTCGGGTCTACTGGGTAACCCGAACTTGTGCAGTCCGGATCTGGATCACTTCCCAACCTGCTCCAAACCCAAATCTCCTACCCCAATTTTGATAGTCATTTTATCCGTTTGTGTTGTTCTGGTCGTCGGGTCGGTTCTTTCGTTTTTGAAATATCGATCCAAAGCACTTGGCGGTAAAACGAAGCGGCTCTACAGGCTCACCACATTCCAGCGGGTCGGGTTTAACGAAGAGGAGGTGATGCAATCATTAACGGAGGAGAATCTGATTGCAACGGGTGGGTCGGGTCATGTGTACAAAGTGCCACTGAAGACGGGGCAGACCGTGGCGGTTAAGAAGCTATGGGGCGGGGCAAGAAAACCCGAAACTGAATCCGTTTTTAGCTCCGAAGTGGAGACGTTGGGTCGGATCCGACATGGCAATATTGTAAAATTGGTGTTTTGTTGTAGTGGTGAGGAAAGTAGAATTTTGGGTTATGAGTACATGGAGAATGGGAGCTTGGGTGATTGTTTGCATGGAGAAAAGGTTGGGCCTTTGGAGGATTGGGCCAAAAGATTCAACATCGCGGTCGGGTCGGCCTACGGCTTGGCCTACCTCCACCACGACTGTGCTCCGCCCATCGTTCACCGGGACGTAAAGAGTAATAACATACTGTTGGACGAGGACTGGACCCCACGGGTGGCTGATTTCGGACTCGCCAAGACTTTGCAGCGTGACGTGGCGGCCGGATGTGGTACCATGTCACAGATTGCTGGGTCCTACGGATATATCGCGCCGGAGTACGCATACACTCTTAAAGTCACTGAGAAGAGTGATGTCTACAGCTTCGGTGTGGTGCTGTTGGAACTGATAACCGGCAAGAGTCCGAATGACGCGTCCTTTGGTGAGAACACAGACATTGTCAAGTGGGTTAGAGAGGCTGCCATGTCTACTCCTGAAGGAGAAGAAGAAAGCAGCAATGACAACGGTTTCTCCGGCGCCAATCTCTGCCAGATTATTGATCCAAGGATTGATCTGTCGACGCGTGATTACGAAAAGATTGAGAAGGTTTTGAAGGTGGCTCTTCTTTGTACCTCTGCATTCCCCATCAACAGGCCCTCCATGAGAAGGGTGGTTGAATTGCTTAGAGATCAGAAACCATCCGACCGTCCAAAATGA

Protein Analysis

988

Amino Acids

107.87

Weight (kDa)

5.28

Isoelectric Point (pI)

32.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 68 2.7e-12 Leucine rich repeat N-terminal domain
LRR_8 PF13855 147 - 205 1e-06 Leucine rich repeat
LRR_14 PF23598 163 - 275 7.8e-11 Leucine-rich repeat region
LRR_14 PF23598 472 - 560 1.5e-06 Leucine-rich repeat region
Pkinase PF00069 688 - 901 6.2e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 689 - 909 8.4e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65710
fragaria_vesca FvH4_1g18670
malus_domestica MD02G1194100.v1.1 MD05G1283300.v1.1 MD15G1304600.v1.1
prunus_persica Prupe.6G212600_v2.0.a1
pyrus_communis pycom02g15810 pycom15g26710
rosa_chinensis RchiOBHm_Chr2g0109521
rosa_laevigata RLG00000017742
rosa_multiflora Rmu_sc0000332.1_g000032
rosa_roxburghii Rroxscaffold_2G00134180
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2AG212900 Rh2BG223100 Rh2CG214700 Rh2DG218500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
Acc36I ACCTGC 2 cut(s) 505, 1874
Acc65I GGTACC 1 cut(s) 2540
AccB1I GGYRCC 2 cut(s) 2540, 2783
AccI GTMKAC 6 cut(s) 703, 752, 1816, 2625, 2735, 2825
AccII CGCG 5 cut(s) 47, 2372, 2579, 2681, 2830
AccIII TCCGGA 2 cut(s) 1196, 1843
AclI AACGTT 1 cut(s) 249
AcoI YGGCCR 4 cut(s) 81, 496, 1075, 2529
AcsI RAATTY 8 cut(s) 757, 917, 1168, 1201, 1253, 1406, 1690, 2271
AcuI CTGAAG 4 cut(s) 1535, 1789, 2135, 2763
AcyI GRCGYC 3 cut(s) 728, 1272, 2784
AfaI GTAC 8 cut(s) 772, 884, 1769, 2102, 2288, 2542, 2588, 2878
AfiI CCNNNNNNNGG 9 cut(s) 163, 706, 1597, 1627, 1649, 1731, 2485, 2898, 2913
AflIII ACRYGT 3 cut(s) 1548, 2679, 2828
AgeI ACCGGT 1 cut(s) 1700
AhdI GACNNNNNGTC 2 cut(s) 1495, 2208
AjiI CACGTC 2 cut(s) 1465, 2524
AjnI CCWGG 2 cut(s) 723, 1300
AjuI GAANNNNNNNTTGG 2 cut(s) 2288, 2320
AleI CACNNNNGTG 1 cut(s) 2412
AloI GAACNNNNNNTCC 8 cut(s) 1175, 1178, 1207, 1210, 1535, 1538, 1567, 1570
Alw21I GWGCWC 4 cut(s) 164, 384, 1266, 2419
Alw26I GTCTC 5 cut(s) 735, 957, 1354, 1756, 2195
AlwNI CAGNNNCTG 1 cut(s) 520
Ama87I CYCGRG 4 cut(s) 157, 377, 851, 1810
Aor13HI TCCGGA 2 cut(s) 1196, 1843
ApeKI GCWGC 8 cut(s) 14, 71, 1021, 1132, 1344, 2515, 2726, 2760
ApoI RAATTY 8 cut(s) 757, 917, 1168, 1201, 1253, 1406, 1690, 2271
ArsI GACNNNNNNTTYG 4 cut(s) 2666, 2669, 2698, 2701
AsiGI ACCGGT 1 cut(s) 1700
Asp700I GAANNNNTTC 4 cut(s) 905, 1190, 1242, 1550
Asp718I GGTACC 1 cut(s) 2540
AspLEI GCGC 4 cut(s) 49, 1418, 2581, 2786
AsuC2I CCSGG 6 cut(s) 131, 241, 532, 624, 1064, 2435
AsuII TTCGAA 1 cut(s) 1246
AvaI CYCGRG 4 cut(s) 157, 377, 851, 1810
AvaII GGWCC 6 cut(s) 154, 288, 517, 1587, 2475, 2562
BamHI GGATCC 3 cut(s) 166, 1635, 2214
BanI GGYRCC 2 cut(s) 2540, 2783
BanII GRGCYC 2 cut(s) 384, 1266
BarI GAAGNNNNNNTAC 2 cut(s) 1982, 2014
BbsI GAAGAC 1 cut(s) 2123
Bbv12I GWGCWC 4 cut(s) 164, 384, 1266, 2419
BbvI GCAGC 8 cut(s) 26, 58, 1033, 1119, 1356, 2527, 2713, 2772
BccI CCATC 5 cut(s) 329, 722, 2432, 2900, 2953
BceAI ACGGC 6 cut(s) 684, 919, 1285, 1357, 1480, 2404
BcgI CGANNNNNNTGC 2 cut(s) 1407, 1441
BciT130I CCWGG 2 cut(s) 725, 1302
BcnI CCSGG 6 cut(s) 131, 241, 532, 624, 1064, 2435
BcoDI GTCTC 5 cut(s) 735, 957, 1354, 1756, 2195
BfmI CTRYAG 2 cut(s) 1999, 2626
BfoI RGCGCY 1 cut(s) 2787
BfuAI ACCTGC 2 cut(s) 505, 1874
BglI GCCNNNNNGGC 1 cut(s) 80
BglII AGATCT 1 cut(s) 889
BmcAI AGTACT 1 cut(s) 884
Bme1390I CCNGG 8 cut(s) 131, 241, 532, 624, 725, 1064, 1302, 2435
Bme18I GGWCC 6 cut(s) 154, 288, 517, 1587, 2475, 2562
BmeRI GACNNNNNGTC 2 cut(s) 1495, 2208
BmeT110I CYCGRG 4 cut(s) 157, 377, 851, 1810
BmgBI CACGTC 2 cut(s) 1465, 2524
BmrFI CCNGG 8 cut(s) 131, 241, 532, 624, 725, 1064, 1302, 2435
BmrI ACTGGG 3 cut(s) 160, 1585, 1829
BmsI GCATC 3 cut(s) 513, 795, 2037
BmuI ACTGGG 3 cut(s) 160, 1585, 1829
BpiI GAAGAC 1 cut(s) 2123
BpmI CTGGAG 4 cut(s) 278, 647, 863, 1381
Bpu10I CCTNAGC 1 cut(s) 1730
Bpu14I TTCGAA 1 cut(s) 1246
BpuMI CCSGG 6 cut(s) 131, 241, 532, 624, 1064, 2435
Bsa29I ATCGAT 1 cut(s) 1963
BsaBI GATNNNNATC 2 cut(s) 171, 1851
BsaHI GRCGYC 3 cut(s) 728, 1272, 2784
BsaJI CCNNGG 4 cut(s) 1301, 2130, 2480, 2810
BsaWI WCCGGW 8 cut(s) 706, 767, 911, 1152, 1196, 1556, 1700, 1843
Bsc4I CCNNNNNNNGG 9 cut(s) 163, 706, 1597, 1627, 1649, 1731, 2485, 2898, 2913
Bse118I RCCGGY 7 cut(s) 83, 473, 498, 545, 1660, 1700, 2660
Bse1I ACTGG 7 cut(s) 155, 188, 880, 1189, 1591, 1824, 2477
Bse3DI GCAATG 3 cut(s) 407, 1408, 2770
Bse8I GATNNNNATC 2 cut(s) 171, 1851
BseAI TCCGGA 2 cut(s) 1196, 1843
BseBI CCWGG 2 cut(s) 725, 1302
BseCI ATCGAT 1 cut(s) 1963
BseDI CCNNGG 4 cut(s) 1301, 2130, 2480, 2810
BseGI GGATG 2 cut(s) 2540, 2945
BseJI GATNNNNATC 2 cut(s) 171, 1851
BseLI CCNNNNNNNGG 9 cut(s) 163, 706, 1597, 1627, 1649, 1731, 2485, 2898, 2913
BseMI GCAATG 3 cut(s) 407, 1408, 2770
BseMII CTCAG 5 cut(s) 107, 589, 706, 1744, 2601
BseNI ACTGG 7 cut(s) 155, 188, 880, 1189, 1591, 1824, 2477
BseRI GAGGAG 3 cut(s) 1556, 2054, 2078
BseX3I CGGCCG 3 cut(s) 496, 1075, 2529
BseXI GCAGC 8 cut(s) 26, 58, 1033, 1119, 1356, 2527, 2713, 2772
BseYI CCCAGC 1 cut(s) 2558
BsgI GTGCAG 1 cut(s) 1858
Bsh1236I CGCG 5 cut(s) 47, 2372, 2579, 2681, 2830
Bsh1285I CGRYCG 5 cut(s) 499, 1078, 2376, 2532, 2953
BshNI GGYRCC 2 cut(s) 2540, 2783
BshTI ACCGGT 1 cut(s) 1700
BshVI ATCGAT 1 cut(s) 1963
BsiEI CGRYCG 5 cut(s) 499, 1078, 2376, 2532, 2953
BsiHKAI GWGCWC 4 cut(s) 164, 384, 1266, 2419
BsiHKCI CYCGRG 4 cut(s) 157, 377, 851, 1810
BslFI GGGAC 3 cut(s) 140, 1785, 2450
BslI CCNNNNNNNGG 9 cut(s) 163, 706, 1597, 1627, 1649, 1731, 2485, 2898, 2913
BsmAI GTCTC 5 cut(s) 735, 957, 1354, 1756, 2195
BsmBI CGTCTC 4 cut(s) 735, 1354, 1756, 2195
BsmFI GGGAC 3 cut(s) 140, 1785, 2450
BsmI GAATGC 2 cut(s) 282, 2885
BsoBI CYCGRG 4 cut(s) 157, 377, 851, 1810
Bsp119I TTCGAA 1 cut(s) 1246
Bsp1286I GDGCHC 4 cut(s) 164, 384, 1266, 2419
Bsp13I TCCGGA 2 cut(s) 1196, 1843
Bsp1407I TGTACA 1 cut(s) 2100
BspCNI CTCAG 5 cut(s) 106, 588, 705, 1743, 2602
BspDI ATCGAT 1 cut(s) 1963
BspEI TCCGGA 2 cut(s) 1196, 1843
BspFNI CGCG 5 cut(s) 47, 2372, 2579, 2681, 2830
BspMI ACCTGC 2 cut(s) 505, 1874
BspQI GCTCTTC 1 cut(s) 2874
BspT104I TTCGAA 1 cut(s) 1246
BspT107I GGYRCC 2 cut(s) 2540, 2783
BsrDI GCAATG 3 cut(s) 407, 1408, 2770
BsrFI RCCGGY 7 cut(s) 83, 473, 498, 545, 1660, 1700, 2660
BsrGI TGTACA 1 cut(s) 2100
BsrI ACTGG 7 cut(s) 155, 188, 880, 1189, 1591, 1824, 2477
BssAI RCCGGY 7 cut(s) 83, 473, 498, 545, 1660, 1700, 2660
BssECI CCNNGG 4 cut(s) 1301, 2130, 2480, 2810
BssNAI GTATAC 1 cut(s) 704
BssNI GRCGYC 3 cut(s) 728, 1272, 2784
BssT1I CCWWGG 1 cut(s) 2810
Bst1107I GTATAC 1 cut(s) 704
Bst2UI CCWGG 2 cut(s) 725, 1302
Bst4CI ACNGT 9 cut(s) 67, 123, 1767, 1799, 2131, 2414, 2460, 2774, 2954
Bst6I CTCTTC 4 cut(s) 401, 2031, 2609, 2874
BstACI GRCGYC 3 cut(s) 728, 1272, 2784
BstAUI TGTACA 1 cut(s) 2100
BstBI TTCGAA 1 cut(s) 1246
BstC8I GCNNGC 8 cut(s) 51, 55, 85, 629, 784, 802, 986, 1130
BstDEI CTNAG 7 cut(s) 93, 575, 692, 886, 1730, 2610, 2930
BstDSI CCRYGG 2 cut(s) 2130, 2480
BstEII GGTNACC 1 cut(s) 1823
BstF5I GGATG 2 cut(s) 2540, 2945
BstFNI CGCG 5 cut(s) 47, 2372, 2579, 2681, 2830
BstH2I RGCGCY 1 cut(s) 2787
BstHHI GCGC 4 cut(s) 49, 1418, 2581, 2786
BstMAI GTCTC 5 cut(s) 735, 957, 1354, 1756, 2195
BstMCI CGRYCG 5 cut(s) 499, 1078, 2376, 2532, 2953
BstMWI GCNNNNNNNGC 5 cut(s) 80, 628, 817, 1053, 2002
BstNI CCWGG 2 cut(s) 725, 1302
BstNSI RCATGY 4 cut(s) 57, 201, 1288, 1552
BstPI GGTNACC 1 cut(s) 1823
BstSCI CCNGG 8 cut(s) 129, 239, 530, 622, 723, 1062, 1300, 2433
BstSFI CTRYAG 2 cut(s) 1999, 2626
BstUI CGCG 5 cut(s) 47, 2372, 2579, 2681, 2830
BstV1I GCAGC 8 cut(s) 26, 58, 1033, 1119, 1356, 2527, 2713, 2772
BstV2I GAAGAC 1 cut(s) 2123
BstX2I RGATCY 6 cut(s) 166, 889, 1635, 1678, 1846, 2214
BstXI CCANNNNNNTGG 2 cut(s) 183, 565
BstYI RGATCY 6 cut(s) 166, 889, 1635, 1678, 1846, 2214
BstZ17I GTATAC 1 cut(s) 704
BstZI CGGCCG 3 cut(s) 496, 1075, 2529
Bsu15I ATCGAT 1 cut(s) 1963
BsuTUI ATCGAT 1 cut(s) 1963
BtgI CCRYGG 2 cut(s) 2130, 2480
BtgZI GCGATG 1 cut(s) 2353
BtrI CACGTC 2 cut(s) 1465, 2524
BtsCI GGATG 2 cut(s) 2540, 2945
BtsIMutI CAGTG 4 cut(s) 583, 1581, 2111, 2607
BveI ACCTGC 2 cut(s) 505, 1874
Cac8I GCNNGC 8 cut(s) 51, 55, 85, 629, 784, 802, 986, 1130
CaiI CAGNNNCTG 1 cut(s) 520
CfoI GCGC 4 cut(s) 49, 1418, 2581, 2786
Cfr10I RCCGGY 7 cut(s) 83, 473, 498, 545, 1660, 1700, 2660
ClaI ATCGAT 1 cut(s) 1963
CseI GACGC 8 cut(s) 717, 817, 1261, 1336, 1393, 2670, 2687, 2836
Csp6I GTAC 8 cut(s) 771, 883, 1768, 2101, 2287, 2541, 2587, 2877
CspAI ACCGGT 1 cut(s) 1700
CviQI GTAC 8 cut(s) 771, 883, 1768, 2101, 2287, 2541, 2587, 2877
DdeI CTNAG 7 cut(s) 93, 575, 692, 886, 1730, 2610, 2930
DinI GGCGCC 1 cut(s) 2785
DrdI GACNNNNNNGTC 1 cut(s) 152
DriI GACNNNNNGTC 2 cut(s) 1495, 2208
DseDI GACNNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 4 cut(s) 81, 496, 1075, 2529
EagI CGGCCG 3 cut(s) 496, 1075, 2529
Eam1104I CTCTTC 4 cut(s) 401, 2031, 2609, 2874
Eam1105I GACNNNNNGTC 2 cut(s) 1495, 2208
EarI CTCTTC 4 cut(s) 401, 2031, 2609, 2874
EciI GGCGGA 3 cut(s) 807, 1730, 2409
Ecl136II GAGCTC 2 cut(s) 382, 1264
EclXI CGGCCG 3 cut(s) 496, 1075, 2529
Eco130I CCWWGG 1 cut(s) 2810
Eco24I GRGCYC 2 cut(s) 384, 1266
Eco47I GGWCC 6 cut(s) 154, 288, 517, 1587, 2475, 2562
Eco52I CGGCCG 3 cut(s) 496, 1075, 2529
Eco53kI GAGCTC 2 cut(s) 382, 1264
Eco57I CTGAAG 4 cut(s) 1535, 1789, 2135, 2763
Eco88I CYCGRG 4 cut(s) 157, 377, 851, 1810
Eco91I GGTNACC 1 cut(s) 1823
EcoICRI GAGCTC 2 cut(s) 382, 1264
EcoO109I RGGNCCY 4 cut(s) 154, 517, 2562, 2901
EcoO65I GGTNACC 1 cut(s) 1823
EcoRI GAATTC 2 cut(s) 757, 1253
EcoRII CCWGG 2 cut(s) 723, 1300
EcoT14I CCWWGG 1 cut(s) 2810
EcoT38I GRGCYC 2 cut(s) 384, 1266
EgeI GGCGCC 1 cut(s) 2785
EheI GGCGCC 1 cut(s) 2785
ErhI CCWWGG 1 cut(s) 2810
Esp3I CGTCTC 4 cut(s) 735, 1354, 1756, 2195
FaqI GGGAC 3 cut(s) 140, 1785, 2450
FauI CCCGC 4 cut(s) 251, 514, 2013, 2147
FblI GTMKAC 6 cut(s) 703, 752, 1816, 2625, 2735, 2825
FokI GGATG 2 cut(s) 2547, 2932
FriOI GRGCYC 2 cut(s) 384, 1266
GlaI GCGC 4 cut(s) 48, 1417, 2580, 2785
GsaI CCCAGC 1 cut(s) 2562
GsuI CTGGAG 4 cut(s) 278, 647, 863, 1381
HaeII RGCGCY 1 cut(s) 2787
HgaI GACGC 8 cut(s) 717, 817, 1261, 1336, 1393, 2670, 2687, 2836
HhaI GCGC 4 cut(s) 49, 1418, 2581, 2786
Hin1I GRCGYC 3 cut(s) 728, 1272, 2784
Hin6I GCGC 4 cut(s) 47, 1416, 2579, 2784
HinP1I GCGC 4 cut(s) 47, 1416, 2579, 2784
HincII GTYRAC 5 cut(s) 316, 585, 1596, 1722, 2826
HindII GTYRAC 5 cut(s) 316, 585, 1596, 1722, 2826
HindIII AAGCTT 1 cut(s) 1439
HpyAV CCTTC 6 cut(s) 645, 1813, 2738, 2845, 2854, 2907
HpyCH4III ACNGT 9 cut(s) 67, 123, 1767, 1799, 2131, 2414, 2460, 2774, 2954
HpyCH4IV ACGT 8 cut(s) 249, 1214, 1242, 1464, 1749, 2204, 2439, 2523
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 628, 817, 1053, 2002
HpyF3I CTNAG 7 cut(s) 93, 575, 692, 886, 1730, 2610, 2930
HpySE526I ACGT 8 cut(s) 249, 1214, 1242, 1464, 1749, 2204, 2439, 2523
Hsp92I GRCGYC 3 cut(s) 728, 1272, 2784
HspAI GCGC 4 cut(s) 47, 1416, 2579, 2784
KasI GGCGCC 1 cut(s) 2783
KflI GGGWCCC 1 cut(s) 154
Kpn2I TCCGGA 2 cut(s) 1196, 1843
KpnI GGTACC 1 cut(s) 2544
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
LguI GCTCTTC 1 cut(s) 2874
LmnI GCTCC 9 cut(s) 365, 666, 1061, 1510, 1600, 1874, 2195, 2301, 2422
Lsp1109I GCAGC 8 cut(s) 26, 58, 1033, 1119, 1356, 2527, 2713, 2772
LweI GCATC 3 cut(s) 513, 795, 2037
MaeII ACGT 8 cut(s) 249, 1214, 1242, 1464, 1749, 2204, 2439, 2523
MaeIII GTNAC 6 cut(s) 805, 1148, 1823, 2519, 2547, 2605
MfeI CAATTG 2 cut(s) 938, 944
MflI RGATCY 6 cut(s) 166, 889, 1635, 1678, 1846, 2214
MhlI GDGCHC 4 cut(s) 164, 384, 1266, 2419
MluI ACGCGT 2 cut(s) 2679, 2828
Mly113I GGCGCC 1 cut(s) 2784
MlyI GAGTC 6 cut(s) 209, 580, 704, 959, 2493, 2677
MmeI TCCRAC 6 cut(s) 150, 382, 2192, 2242, 2442, 2628
MroI TCCGGA 2 cut(s) 1196, 1843
MroNI GCCGGC 1 cut(s) 83
MroXI GAANNNNTTC 4 cut(s) 905, 1190, 1242, 1550
MseI TTAA 7 cut(s) 62, 246, 1217, 2031, 2057, 2139, 2601
MslI CAYNNNNRTG 2 cut(s) 369, 2412
MspA1I CMGCKG 2 cut(s) 17, 2020
MspR9I CCNGG 8 cut(s) 131, 241, 532, 624, 725, 1064, 1302, 2435
MunI CAATTG 2 cut(s) 938, 944
Mva1269I GAATGC 2 cut(s) 282, 2885
MvaI CCWGG 2 cut(s) 725, 1302
MvnI CGCG 5 cut(s) 47, 2372, 2579, 2681, 2830
MwoI GCNNNNNNNGC 5 cut(s) 80, 628, 817, 1053, 2002
NaeI GCCGGC 1 cut(s) 85
NarI GGCGCC 1 cut(s) 2784
NciI CCSGG 6 cut(s) 131, 241, 532, 624, 1064, 2435
NgoMIV GCCGGC 1 cut(s) 83
NmeAIII GCCGAG 2 cut(s) 1053, 1629
NmuCI GTSAC 4 cut(s) 805, 2519, 2547, 2605
NspI RCATGY 4 cut(s) 57, 201, 1288, 1552
NspV TTCGAA 1 cut(s) 1246
OliI CACNNNNGTG 1 cut(s) 2412
PaeI GCATGC 1 cut(s) 57
PaeR7I CTCGAG 1 cut(s) 377
PciI ACATGT 1 cut(s) 1548
PciSI GCTCTTC 1 cut(s) 2874
PcsI WCGNNNNNNNCGW 8 cut(s) 141, 779, 1248, 1272, 1388, 1451, 1946, 2031
PctI GAATGC 2 cut(s) 282, 2885
PdiI GCCGGC 1 cut(s) 85
PdmI GAANNNNTTC 4 cut(s) 905, 1190, 1242, 1550
PflFI GACNNNGTC 3 cut(s) 152, 1271, 2705
PfoI TCCNGGA 2 cut(s) 530, 1062
PinAI ACCGGT 1 cut(s) 1700
PleI GAGTC 6 cut(s) 209, 580, 704, 958, 2493, 2676
PluTI GGCGCC 1 cut(s) 2787
PpsI GAGTC 6 cut(s) 209, 580, 704, 958, 2493, 2676
PpuMI RGGWCCY 3 cut(s) 154, 517, 2562
PscI ACATGT 1 cut(s) 1548
Psp124BI GAGCTC 2 cut(s) 384, 1266
Psp1406I AACGTT 1 cut(s) 249
Psp5II RGGWCCY 3 cut(s) 154, 517, 2562
Psp6I CCWGG 2 cut(s) 723, 1300
PspEI GGTNACC 1 cut(s) 1823
PspFI CCCAGC 1 cut(s) 2558
PspGI CCWGG 2 cut(s) 723, 1300
PspPPI RGGWCCY 3 cut(s) 154, 517, 2562
PstNI CAGNNNCTG 1 cut(s) 520
PsuI RGATCY 6 cut(s) 166, 889, 1635, 1678, 1846, 2214
PsyI GACNNNGTC 3 cut(s) 152, 1271, 2705
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 8 cut(s) 772, 884, 1769, 2102, 2288, 2542, 2588, 2878
RsaNI GTAC 8 cut(s) 771, 883, 1768, 2101, 2287, 2541, 2587, 2877
RseI CAYNNNNRTG 2 cut(s) 369, 2412
SacI GAGCTC 2 cut(s) 384, 1266
SalI GTCGAC 1 cut(s) 2824
SapI GCTCTTC 1 cut(s) 2874
SaqAI TTAA 7 cut(s) 62, 246, 1217, 2031, 2057, 2139, 2601
ScaI AGTACT 1 cut(s) 884
SchI GAGTC 6 cut(s) 209, 580, 704, 959, 2493, 2677
ScrFI CCNGG 8 cut(s) 131, 241, 532, 624, 725, 1064, 1302, 2435
SduI GDGCHC 4 cut(s) 164, 384, 1266, 2419
SfaNI GCATC 3 cut(s) 513, 795, 2037
SfcI CTRYAG 2 cut(s) 1999, 2626
SfoI GGCGCC 1 cut(s) 2785
Sfr274I CTCGAG 1 cut(s) 377
SfuI TTCGAA 1 cut(s) 1246
SgrAI CRCCGGYG 2 cut(s) 473, 1700
SinI GGWCC 6 cut(s) 154, 288, 517, 1587, 2475, 2562
SlaI CTCGAG 1 cut(s) 377
SmiMI CAYNNNNRTG 2 cut(s) 369, 2412
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SphI GCATGC 1 cut(s) 57
SspDI GGCGCC 1 cut(s) 2783
SspI AATATT 1 cut(s) 2230
SstI GAGCTC 2 cut(s) 384, 1266
StyD4I CCNGG 8 cut(s) 129, 239, 530, 622, 723, 1062, 1300, 2433
StyI CCWWGG 1 cut(s) 2810
TaaI ACNGT 9 cut(s) 67, 123, 1767, 1799, 2131, 2414, 2460, 2774, 2954
TaiI ACGT 8 cut(s) 252, 1217, 1245, 1467, 1752, 2207, 2442, 2526
TatI WGTACW 3 cut(s) 882, 2100, 2286
TauI GCSGC 4 cut(s) 330, 1370, 1996, 2531
Tru1I TTAA 7 cut(s) 62, 246, 1217, 2031, 2057, 2139, 2601
Tru9I TTAA 7 cut(s) 62, 246, 1217, 2031, 2057, 2139, 2601
TscAI CASTG 4 cut(s) 583, 1581, 2118, 2614
TseFI GTSAC 4 cut(s) 805, 2519, 2547, 2605
TseI GCWGC 8 cut(s) 14, 71, 1021, 1132, 1344, 2515, 2726, 2760
Tsp45I GTSAC 4 cut(s) 805, 2519, 2547, 2605
TspDTI ATGAA 5 cut(s) 445, 632, 914, 1337, 1419
TspRI CASTG 4 cut(s) 583, 1581, 2118, 2614
Tth111I GACNNNGTC 3 cut(s) 152, 1271, 2705
VpaK11BI GGWCC 6 cut(s) 154, 288, 517, 1587, 2475, 2562
XapI RAATTY 8 cut(s) 757, 917, 1168, 1201, 1253, 1406, 1690, 2271
XceI RCATGY 4 cut(s) 57, 201, 1288, 1552
XcmI CCANNNNNNNNNTGG 3 cut(s) 721, 1628, 2914
XhoI CTCGAG 1 cut(s) 377
XmiI GTMKAC 6 cut(s) 703, 752, 1816, 2625, 2735, 2825
XmnI GAANNNNTTC 4 cut(s) 905, 1190, 1242, 1550
ZrmI AGTACT 1 cut(s) 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.