Rroxscaffold_2G00134180

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014085
Physical Location & Seq
Forward (+)
71653001 .. 71657189
4189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00134180.1

Sequence Viewer

Length: 2964 bp
ATGATGACACTTTTAGCTCCAAAACCGCTGCTGATTCTCTGTACCCTATCAATTTACTTCTCCTACGTTATGGCCTTTTCGGCCGGAGACACCCAGATCCTGCTCCGGGTAAAAACCCAACTCGACGACCCGGATGGAAACCTCAGCGACTGGGTTTCCGGCAGCAATCACAGTCCATGCAATTGGTCAGGAATCACTTGTGAGCAGAAAAATTTCTCCATTGTTTCAATAGACATGTCCGAGTTTGGTGTTCACGGCGGATTTCCAGCTGAGTTGTGCCGGATTCGGACCCTTCAGAACCTCACTTTAAACTTCAACTCCATCAACGGTACACTCTCCACTAAGCTCCTGTCTCTCTGCTCACACCTTCAAGCTTTGGAGCTCGAGGAGAACGATATCGTCGGAGAATTGCCGGAATTTCCACCCGAGTTTACTGGTTTACGAGTCTTGAATCTCCAAACCAACAACTTTACTGGAGATATCCCGGCGAGTTTCGGGCGGTTGCCGCAACTGAGAGTGCTGAAGCTTTCCGGGAACTTGCTCACCGGTCCGTTTCCAAAGTTCTTGACCAACCTGAGTGAGTTGACTCATTTGGATATTGCTTACAATCCAACAATGAAGCCCAGTTTGCTCCCTCCAGAAATCGGAAACTTAACCAATCTCCAGGAGCTGGTTATCTTCAATATCAATCTAACAGGCAATATTCCGGACTCCATCGGAAACCTGGTCTCGCTCAGAAACCTGGACTTGTCCCAGAATTCGTTATCCGGGACAATTCCAGAGAGCATTGGAAGATTGAGAAGTGCACAACAAATAGAGCTCTATTTGAACCGATTGTACGGCGAATTGCCGGACAGTATTGCAAACTTGACGTCTCTGCAGAACTTGGATTTGTCACAGAACGGATTCACCGGCAAATTTTCTGAAAGAATCGCCGGAATTCGTTTCGTTTCTCTGCGACTGGCCGATAACCTTCTAGAGGGCTCTGTTCCGGAGATCGTAGCCAACAGTCCTGATCTGGTTCAGTTGCATCTCTTCAATAACAGCTTTTCCGGTTCACTCCCGGAAAATCTCGGCCGAAATTCAGCTCTGCGAGATTTAGATGTCTCAACCAATAAATTCACCGGCGAGCTGCCTCGGCATCTCTGCTACGGTAAAAATCTCAGCAGTCTGGTTATGTTCGGGAACCAATTCTCTGGAAATTTACCGGATACACTAAGCGAGTGTCAATCCCTGAACTATGTTCGAATTGAACAGAATGAACTCTCCGGTCAAGTGTCGGACAAGTTTTGGGGTCTGCCTCTGCTTACAGATCTTCGAATGCATAACAACAGACTCAGCGGACCGGTTTCGTCTTCTATTTCCTTTGCTAATGGTCTCGAAGCCCTTCAACTCTCTGGCAACGTCTTCTCCGGCCAGCTTCCACCGCAGATTTGCAAGTTGTCCAAGCTGGTAGAGCTGGACGTCAGCGAAAATCAGTTTTCCGGTGAAGTTCCGTCTTGTATGACAGAGCTGAAGAATCTTCAGAAGCTTAGGATGCAGCAGAATTTGTTCACCGGTGAGATTCCAAGACATATGAGTTCATGGACCCAGTTAACCGAGTTGAACCTATCCAAAAACCGATTCTCAGGTCCAATTCCTCCGGAACTGGGTGACTTACCGGTGCTGAATTACTTGGACCTCTCTGATAACTCACTCACCGGTGAGATTCCGGTGGATTTGACCAAGCTCAAGCTCGGCCAGTTCAATCTCTCAGACAACAAACTGTACGGTGAGATACCAACTGGGCTGGACTACGAGCTCTTCGCACCGGGTCTACTGGGCAACCCGGGTCTATGCAGTTCGAATTTAAAGCAGGTTCATCCATGTCAGAGATCCAAATCCCCTACTCTTTTATTGGCTGTTATTCTCTCCATTTGCGTTGTACTGCTGGTTGGATCTGTTCTTTGGTATCTGAAAATTAGAGCACAAGTTTTTGGCGGTAAAGCCAAACGGCTTTACAGTGTCACCTCTTTTCAACGGGTCGGGTTCAATGAAGACGATGTGTTACCCTCGTTAACCAATGAGAATCTGATTGCAACGGGCGGGTCGGGTCATGTTTACAGAGTGAAGTTGAAGTCGGGCCAAGTTTTAGCAGTTAAGAAGCTATGGGGTGGAAGTAGCAGGAAGCCGGAAAGTGAGTTGGTTTTTAGATCCGAGGTGCAGACGCTGGGTCGGATCCGGCATGGCAATATCGTAAAATTGGTGTTCTGTTGTAGTGGGGAGGATTGTAGGATGTTGGCCTATGAGTACATGGTGAATGGGAGCTTGGGTGATGCTTTGCATGGAGAGAAGCTTGGGCCTTTGGTGGACTGGGCCGCGAGGTTCAACGTTGCTTTGGGCTCGGCCCACGGGCTGGCCTATCTTCATCACGACTGTGTTCCGGCCATCGTGCACCGGGATGTGAAGAGTAACAACATATTGCTCGACGAGGAGTGGACACCGCGGGTGGCGGATTTCGGGCTCGCCAAGACGTTGGAGCGTGACGTGGCATCCGGACATGGTGCCATGTCAAGGATAGCTGGATCCTACGGCTATATTGCGCCAGAATATGCATACACACTAAAAGTTACTGAGAAGAGCGACGTCTACAGCTTCGGTGTGGTGTTACTGGAACTGATCACCGGTAAGAGGCCGAATGACTTATCCTTTGGGGAGAACAAGGACATAGTGAAGTGGATTACTGAGGCTGCATTGTCTTCTACTTCCTCTTGTGATGGGGACGGTGAAAATGAAGAAGGTTGTGGGATTGATCTTAGCCAGATTGTTGACCCAAGAATGAACCCAACTACAAGGGATTTTGAAGAGATTGAGAATGTTTTGAGTGTGGCTCTTCTTTGCACCTCTGCGTTCCCAATTAACAGGCCATCCATGAGAAGAGTGGTCGAATTGCTAAAGGATCAGAAACCTGCTAGTCAAACATGA

Protein Analysis

987

Amino Acids

108.41

Weight (kDa)

5.45

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 67 8.3e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 96 - 226 2.9e-07 Leucine-rich repeat region
LRR_14 PF23598 143 - 275 3.5e-11 Leucine-rich repeat region
LRR_8 PF13855 146 - 204 1.7e-09 Leucine rich repeat
LRR_Zer-1 PF25013 188 - 254 8.8e-06 Zer-1-like, Leucine-rich repeats
LRR_14 PF23598 234 - 445 4.8e-10 Leucine-rich repeat region
LRR_14 PF23598 463 - 561 2.5e-07 Leucine-rich repeat region
Pkinase PF00069 689 - 897 7.5e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 689 - 908 1.8e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65710
fragaria_vesca FvH4_1g18670
malus_domestica MD02G1194100.v1.1 MD05G1283300.v1.1 MD15G1304600.v1.1
prunus_persica Prupe.6G212600_v2.0.a1
pyrus_communis pycom02g15810 pycom15g26710
rosa_chinensis RchiOBHm_Chr2g0109521
rosa_laevigata RLG00000017742
rosa_multiflora Rmu_sc0000332.1_g000032
rosa_roxburghii Rroxscaffold_2G00134180
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2AG212900 Rh2BG223100 Rh2CG214700 Rh2DG218500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 875, 1467, 2628
Acc36I ACCTGC 2 cut(s) 1846, 2956
AccB1I GGYRCC 1 cut(s) 2543
AccB7I CCANNNNNTGG 2 cut(s) 670, 2395
AccI GTMKAC 2 cut(s) 1816, 2628
AccII CGCG 2 cut(s) 2360, 2485
AccIII TCCGGA 4 cut(s) 706, 991, 1642, 2534
AclI AACGTT 1 cut(s) 2370
AclWI GGATC 9 cut(s) 91, 1869, 1945, 2187, 2212, 2225, 2559, 2572, 2946
AcoI YGGCCR 6 cut(s) 81, 963, 1075, 1414, 1738, 2424
AcuI CTGAAG 4 cut(s) 278, 542, 1508, 1535
AcyI GRCGYC 3 cut(s) 872, 1464, 2625
AfaI GTAC 6 cut(s) 43, 331, 839, 1769, 1926, 2291
AfiI CCNNNNNNNGG 8 cut(s) 106, 644, 670, 979, 1649, 2395, 2553, 2670
AflIII ACRYGT 1 cut(s) 234
AgeI ACCGGT 6 cut(s) 545, 1345, 1556, 1660, 1700, 2663
AhdI GACNNNNNGTC 1 cut(s) 2211
AjiI CACGTC 1 cut(s) 2527
AjnI CCWGG 3 cut(s) 663, 723, 741
AjuI GAANNNNNNNTTGG 4 cut(s) 1607, 1639, 2291, 2323
AleI CACNNNNGTG 1 cut(s) 2415
Alw21I GWGCWC 6 cut(s) 384, 808, 822, 1803, 1969, 2436
Alw26I GTCTC 6 cut(s) 81, 357, 733, 879, 1111, 1382
Alw44I GTGCAC 2 cut(s) 804, 2432
AlwI GGATC 9 cut(s) 91, 1869, 1945, 2187, 2212, 2225, 2559, 2572, 2946
AlwNI CAGNNNCTG 4 cut(s) 100, 150, 670, 2209
Ama87I CYCGRG 3 cut(s) 383, 425, 1828
Aor13HI TCCGGA 4 cut(s) 706, 991, 1642, 2534
ApaLI GTGCAC 2 cut(s) 804, 2432
ApeKI GCWGC 5 cut(s) 28, 162, 1132, 1540, 2729
ArsI GACNNNNNNTTYG 4 cut(s) 857, 889, 2672, 2704
AsiGI ACCGGT 6 cut(s) 545, 1345, 1556, 1660, 1700, 2663
Asp700I GAANNNNTTC 5 cut(s) 212, 905, 1190, 1386, 1550
AspLEI GCGC 1 cut(s) 2584
AsuII TTCGAA 3 cut(s) 1246, 1318, 1844
AvaI CYCGRG 3 cut(s) 383, 425, 1828
AvaII GGWCC 6 cut(s) 288, 548, 1343, 1587, 1631, 1678
BaeGI GKGCMC 2 cut(s) 808, 2436
BamHI GGATCC 2 cut(s) 2217, 2564
BanI GGYRCC 1 cut(s) 2543
BanII GRGCYC 6 cut(s) 384, 822, 986, 1803, 2384, 2505
BbsI GAAGAC 4 cut(s) 1347, 1399, 2043, 2730
Bbv12I GWGCWC 6 cut(s) 384, 808, 822, 1803, 1969, 2436
BbvCI CCTCAGC 1 cut(s) 143
BbvI GCAGC 5 cut(s) 15, 174, 1119, 1552, 2716
BccI CCATC 6 cut(s) 128, 329, 722, 2435, 2750, 2914
BceAI ACGGC 4 cut(s) 271, 856, 2009, 2587
BciT130I CCWGG 3 cut(s) 665, 725, 743
BciVI GTATCC 1 cut(s) 1204
BclI TGATCA 1 cut(s) 2658
BcoDI GTCTC 6 cut(s) 81, 357, 733, 879, 1111, 1382
BfaI CTAG 2 cut(s) 977, 2952
BfmI CTRYAG 2 cut(s) 878, 2629
BfuAI ACCTGC 2 cut(s) 1846, 2956
BfuI GTATCC 1 cut(s) 1204
BglI GCCNNNNNGGC 1 cut(s) 80
BglII AGATCT 1 cut(s) 1312
BisI GCNGC 7 cut(s) 29, 163, 506, 1133, 1541, 2358, 2730
BlsI GCNGC 7 cut(s) 30, 164, 507, 1134, 1542, 2359, 2731
Bme18I GGWCC 6 cut(s) 288, 548, 1343, 1587, 1631, 1678
BmeRI GACNNNNNGTC 1 cut(s) 2211
BmeT110I CYCGRG 3 cut(s) 383, 425, 1828
BmgBI CACGTC 1 cut(s) 2527
BmiI GGNNCC 6 cut(s) 290, 1187, 1589, 2219, 2545, 2566
BmrI ACTGGG 7 cut(s) 160, 618, 1585, 1658, 1794, 1829, 2362
BmsI GCATC 5 cut(s) 1039, 1150, 1527, 2305, 2540
BmuI ACTGGG 7 cut(s) 160, 618, 1585, 1658, 1794, 1829, 2362
BpiI GAAGAC 4 cut(s) 1347, 1399, 2043, 2730
BplI GAGNNNNNCTC 2 cut(s) 2854, 2886
BpmI CTGGAG 3 cut(s) 495, 621, 647
Bpu10I CCTNAGC 2 cut(s) 143, 1532
Bpu14I TTCGAA 3 cut(s) 1246, 1318, 1844
BpuEI CTTGAG 1 cut(s) 1715
BsaBI GATNNNNATC 1 cut(s) 1879
BsaHI GRCGYC 3 cut(s) 872, 1464, 2625
BsaI GGTCTC 2 cut(s) 733, 1382
BsaJI CCNNGG 5 cut(s) 1136, 1828, 2196, 2389, 2483
BsaXI ACNNNNNCTCC 6 cut(s) 302, 332, 1394, 1424, 2296, 2326
Bsc4I CCNNNNNNNGG 8 cut(s) 106, 644, 670, 979, 1649, 2395, 2553, 2670
Bse118I RCCGGY 8 cut(s) 545, 911, 1124, 1345, 1556, 1660, 1700, 2663
Bse8I GATNNNNATC 1 cut(s) 1879
BseAI TCCGGA 4 cut(s) 706, 991, 1642, 2534
BseBI CCWGG 3 cut(s) 665, 725, 743
BseDI CCNNGG 5 cut(s) 1136, 1828, 2196, 2389, 2483
BseGI GGATG 7 cut(s) 139, 1542, 1861, 2280, 2446, 2531, 2906
BseJI GATNNNNATC 1 cut(s) 1879
BseLI CCNNNNNNNGG 8 cut(s) 106, 644, 670, 979, 1649, 2395, 2553, 2670
BseRI GAGGAG 2 cut(s) 401, 2486
BseSI GKGCMC 2 cut(s) 808, 2436
BseX3I CGGCCG 2 cut(s) 81, 1075
BseXI GCAGC 5 cut(s) 15, 174, 1119, 1552, 2716
BseYI CCCAGC 1 cut(s) 2209
BsgI GTGCAG 1 cut(s) 2222
Bsh1236I CGCG 2 cut(s) 2360, 2485
Bsh1285I CGRYCG 2 cut(s) 84, 1078
BshNI GGYRCC 1 cut(s) 2543
BshTI ACCGGT 6 cut(s) 545, 1345, 1556, 1660, 1700, 2663
BsiEI CGRYCG 2 cut(s) 84, 1078
BsiHKAI GWGCWC 6 cut(s) 384, 808, 822, 1803, 1969, 2436
BsiHKCI CYCGRG 3 cut(s) 383, 425, 1828
BslFI GGGAC 3 cut(s) 736, 784, 2774
BslI CCNNNNNNNGG 8 cut(s) 106, 644, 670, 979, 1649, 2395, 2553, 2670
BsmAI GTCTC 6 cut(s) 81, 357, 733, 879, 1111, 1382
BsmBI CGTCTC 1 cut(s) 879
BsmFI GGGAC 3 cut(s) 736, 784, 2774
BsmI GAATGC 1 cut(s) 1326
Bso31I GGTCTC 2 cut(s) 733, 1382
BsoBI CYCGRG 3 cut(s) 383, 425, 1828
Bsp119I TTCGAA 3 cut(s) 1246, 1318, 1844
Bsp1286I GDGCHC 9 cut(s) 384, 808, 822, 986, 1803, 1969, 2384, 2436, 2505
Bsp13I TCCGGA 4 cut(s) 706, 991, 1642, 2534
BspEI TCCGGA 4 cut(s) 706, 991, 1642, 2534
BspFNI CGCG 2 cut(s) 2360, 2485
BspLI GGNNCC 6 cut(s) 290, 1187, 1589, 2219, 2545, 2566
BspMAI CTGCAG 1 cut(s) 882
BspMI ACCTGC 2 cut(s) 1846, 2956
BspPI GGATC 9 cut(s) 91, 1869, 1945, 2187, 2212, 2225, 2559, 2572, 2946
BspQI GCTCTTC 3 cut(s) 1808, 2612, 2877
BspT104I TTCGAA 3 cut(s) 1246, 1318, 1844
BspT107I GGYRCC 1 cut(s) 2543
BspTNI GGTCTC 2 cut(s) 733, 1382
BsrFI RCCGGY 8 cut(s) 545, 911, 1124, 1345, 1556, 1660, 1700, 2663
BssAI RCCGGY 8 cut(s) 545, 911, 1124, 1345, 1556, 1660, 1700, 2663
BssECI CCNNGG 5 cut(s) 1136, 1828, 2196, 2389, 2483
BssNI GRCGYC 3 cut(s) 872, 1464, 2625
Bst2UI CCWGG 3 cut(s) 665, 725, 743
Bst6I CTCTTC 7 cut(s) 1040, 1808, 2441, 2612, 2838, 2877, 2911
BstACI GRCGYC 3 cut(s) 872, 1464, 2625
BstBI TTCGAA 3 cut(s) 1246, 1318, 1844
BstC8I GCNNGC 4 cut(s) 1130, 1418, 2397, 2505
BstDSI CCRYGG 2 cut(s) 2389, 2483
BstENI CCTNNNNNAGG 1 cut(s) 977
BstF5I GGATG 7 cut(s) 139, 1542, 1861, 2280, 2446, 2531, 2906
BstFNI CGCG 2 cut(s) 2360, 2485
BstHHI GCGC 1 cut(s) 2584
BstMAI GTCTC 6 cut(s) 81, 357, 733, 879, 1111, 1382
BstMCI CGRYCG 2 cut(s) 84, 1078
BstMWI GCNNNNNNNGC 7 cut(s) 80, 505, 628, 1138, 1426, 1456, 1537
BstNI CCWGG 3 cut(s) 665, 725, 743
BstNSI RCATGY 1 cut(s) 238
BstSFI CTRYAG 2 cut(s) 878, 2629
BstSLI GKGCMC 2 cut(s) 808, 2436
BstUI CGCG 2 cut(s) 2360, 2485
BstV1I GCAGC 5 cut(s) 15, 174, 1119, 1552, 2716
BstV2I GAAGAC 4 cut(s) 1347, 1399, 2043, 2730
BstX2I RGATCY 7 cut(s) 96, 1312, 1874, 1937, 2192, 2217, 2564
BstXI CCANNNNNNTGG 3 cut(s) 183, 1196, 2515
BstYI RGATCY 7 cut(s) 96, 1312, 1874, 1937, 2192, 2217, 2564
BstZI CGGCCG 2 cut(s) 81, 1075
BsuI GTATCC 1 cut(s) 1204
BtgI CCRYGG 2 cut(s) 2389, 2483
BtrI CACGTC 1 cut(s) 2527
BtsCI GGATG 7 cut(s) 139, 1542, 1861, 2280, 2446, 2531, 2906
BtsIMutI CAGTG 1 cut(s) 2008
BveI ACCTGC 2 cut(s) 1846, 2956
Cac8I GCNNGC 4 cut(s) 1130, 1418, 2397, 2505
CaiI CAGNNNCTG 4 cut(s) 100, 150, 670, 2209
CfoI GCGC 1 cut(s) 2584
Cfr10I RCCGGY 8 cut(s) 545, 911, 1124, 1345, 1556, 1660, 1700, 2663
Cfr42I CCGCGG 1 cut(s) 2486
Cfr9I CCCGGG 1 cut(s) 1828
CpoI CGGWCCG 2 cut(s) 548, 1343
CseI GACGC 1 cut(s) 2215
CsiI ACCWGGT 1 cut(s) 723
Csp6I GTAC 6 cut(s) 42, 330, 838, 1768, 1925, 2290
CspAI ACCGGT 6 cut(s) 545, 1345, 1556, 1660, 1700, 2663
CspI CGGWCCG 2 cut(s) 548, 1343
CviQI GTAC 6 cut(s) 42, 330, 838, 1768, 1925, 2290
DraI TTTAAA 2 cut(s) 309, 1851
DriI GACNNNNNGTC 1 cut(s) 2211
EaeI YGGCCR 6 cut(s) 81, 963, 1075, 1414, 1738, 2424
EagI CGGCCG 2 cut(s) 81, 1075
Eam1104I CTCTTC 7 cut(s) 1040, 1808, 2441, 2612, 2838, 2877, 2911
Eam1105I GACNNNNNGTC 1 cut(s) 2211
EarI CTCTTC 7 cut(s) 1040, 1808, 2441, 2612, 2838, 2877, 2911
EciI GGCGGA 2 cut(s) 273, 2507
Ecl136II GAGCTC 3 cut(s) 382, 820, 1801
EclXI CGGCCG 2 cut(s) 81, 1075
Eco24I GRGCYC 6 cut(s) 384, 822, 986, 1803, 2384, 2505
Eco31I GGTCTC 2 cut(s) 733, 1382
Eco32I GATATC 2 cut(s) 397, 481
Eco47I GGWCC 6 cut(s) 288, 548, 1343, 1587, 1631, 1678
Eco52I CGGCCG 2 cut(s) 81, 1075
Eco53kI GAGCTC 3 cut(s) 382, 820, 1801
Eco57I CTGAAG 4 cut(s) 278, 542, 1508, 1535
Eco88I CYCGRG 3 cut(s) 383, 425, 1828
EcoICRI GAGCTC 3 cut(s) 382, 820, 1801
EcoNI CCTNNNNNAGG 1 cut(s) 977
EcoRI GAATTC 2 cut(s) 757, 939
EcoRII CCWGG 3 cut(s) 663, 723, 741
EcoRV GATATC 2 cut(s) 397, 481
EcoT22I ATGCAT 2 cut(s) 1326, 2596
EcoT38I GRGCYC 6 cut(s) 384, 822, 986, 1803, 2384, 2505
Esp3I CGTCTC 1 cut(s) 879
FaqI GGGAC 3 cut(s) 736, 784, 2774
FauI CCCGC 2 cut(s) 2078, 2478
FauNDI CATATG 1 cut(s) 1575
FbaI TGATCA 1 cut(s) 2658
FblI GTMKAC 2 cut(s) 1816, 2628
Fnu4HI GCNGC 7 cut(s) 29, 163, 506, 1133, 1541, 2358, 2730
FokI GGATG 7 cut(s) 146, 1549, 1848, 2287, 2453, 2518, 2893
FriOI GRGCYC 6 cut(s) 384, 822, 986, 1803, 2384, 2505
Fsp4HI GCNGC 7 cut(s) 29, 163, 506, 1133, 1541, 2358, 2730
FspBI CTAG 2 cut(s) 977, 2952
GlaI GCGC 1 cut(s) 2583
GluI GCNGC 7 cut(s) 29, 163, 506, 1133, 1541, 2358, 2730
GsaI CCCAGC 1 cut(s) 2213
GsuI CTGGAG 3 cut(s) 495, 621, 647
HgaI GACGC 1 cut(s) 2215
HhaI GCGC 1 cut(s) 2584
Hin1I GRCGYC 3 cut(s) 872, 1464, 2625
Hin6I GCGC 1 cut(s) 2582
HinP1I GCGC 1 cut(s) 2582
HincII GTYRAC 4 cut(s) 585, 1596, 2058, 2809
HindII GTYRAC 4 cut(s) 585, 1596, 2058, 2809
HindIII AAGCTT 4 cut(s) 372, 524, 1529, 2333
HpaI GTTAAC 2 cut(s) 1596, 2058
Hpy99I CGWCG 4 cut(s) 128, 404, 2471, 2627
HpyAV CCTTC 5 cut(s) 302, 377, 983, 1397, 2771
HpyCH4IV ACGT 8 cut(s) 66, 872, 1404, 1464, 2370, 2513, 2526, 2625
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 505, 628, 1138, 1426, 1456, 1537
HpySE526I ACGT 8 cut(s) 66, 872, 1404, 1464, 2370, 2513, 2526, 2625
Hsp92I GRCGYC 3 cut(s) 872, 1464, 2625
HspAI GCGC 1 cut(s) 2582
Kpn2I TCCGGA 4 cut(s) 706, 991, 1642, 2534
Ksp22I TGATCA 1 cut(s) 2658
KspAI GTTAAC 2 cut(s) 1596, 2058
KspI CCGCGG 1 cut(s) 2486
LguI GCTCTTC 3 cut(s) 1808, 2612, 2877
LmnI GCTCC 8 cut(s) 22, 108, 351, 379, 636, 667, 2304, 2518
Lsp1109I GCAGC 5 cut(s) 15, 174, 1119, 1552, 2716
LweI GCATC 5 cut(s) 1039, 1150, 1527, 2305, 2540
MabI ACCWGGT 1 cut(s) 723
MaeI CTAG 2 cut(s) 977, 2952
MaeII ACGT 8 cut(s) 66, 872, 1404, 1464, 2370, 2513, 2526, 2625
MaeIII GTNAC 8 cut(s) 894, 1652, 2005, 2046, 2450, 2522, 2608, 2646
MfeI CAATTG 1 cut(s) 181
MflI RGATCY 7 cut(s) 96, 1312, 1874, 1937, 2192, 2217, 2564
MhlI GDGCHC 9 cut(s) 384, 808, 822, 986, 1803, 1969, 2384, 2436, 2505
MlyI GAGTC 4 cut(s) 453, 580, 704, 1329
MmeI TCCRAC 6 cut(s) 382, 635, 1260, 1915, 2195, 2496
Mph1103I ATGCAT 2 cut(s) 1326, 2596
MroI TCCGGA 4 cut(s) 706, 991, 1642, 2534
MroXI GAANNNNTTC 5 cut(s) 212, 905, 1190, 1386, 1550
MseI TTAA 7 cut(s) 308, 653, 1595, 1850, 2057, 2139, 2898
MslI CAYNNNNRTG 2 cut(s) 2415, 2439
MspA1I CMGCKG 4 cut(s) 28, 269, 1341, 2485
MunI CAATTG 1 cut(s) 181
Mva1269I GAATGC 1 cut(s) 1326
MvaI CCWGG 3 cut(s) 665, 725, 743
MvnI CGCG 2 cut(s) 2360, 2485
MwoI GCNNNNNNNGC 7 cut(s) 80, 505, 628, 1138, 1426, 1456, 1537
NdeI CATATG 1 cut(s) 1575
NlaIV GGNNCC 6 cut(s) 290, 1187, 1589, 2219, 2545, 2566
NmeAIII GCCGAG 4 cut(s) 1053, 1117, 1716, 2363
NmuCI GTSAC 4 cut(s) 894, 1652, 2005, 2522
NsiI ATGCAT 2 cut(s) 1326, 2596
NspI RCATGY 1 cut(s) 238
NspV TTCGAA 3 cut(s) 1246, 1318, 1844
OliI CACNNNNGTG 1 cut(s) 2415
PaeR7I CTCGAG 1 cut(s) 383
PciI ACATGT 1 cut(s) 234
PciSI GCTCTTC 3 cut(s) 1808, 2612, 2877
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PctI GAATGC 1 cut(s) 1326
PdmI GAANNNNTTC 5 cut(s) 212, 905, 1190, 1386, 1550
PflMI CCANNNNNTGG 2 cut(s) 670, 2395
PfoI TCCNGGA 4 cut(s) 530, 663, 767, 1062
PinAI ACCGGT 6 cut(s) 545, 1345, 1556, 1660, 1700, 2663
PkrI GCNGC 7 cut(s) 30, 164, 507, 1134, 1542, 2359, 2731
PleI GAGTC 4 cut(s) 452, 580, 704, 1329
PpsI GAGTC 4 cut(s) 452, 580, 704, 1329
PscI ACATGT 1 cut(s) 234
Psp124BI GAGCTC 3 cut(s) 384, 822, 1803
Psp1406I AACGTT 1 cut(s) 2370
Psp6I CCWGG 3 cut(s) 663, 723, 741
PspFI CCCAGC 1 cut(s) 2209
PspGI CCWGG 3 cut(s) 663, 723, 741
PspN4I GGNNCC 6 cut(s) 290, 1187, 1589, 2219, 2545, 2566
PspXI VCTCGAGB 1 cut(s) 383
PsrI GAACNNNNNNTAC 2 cut(s) 821, 853
PstI CTGCAG 1 cut(s) 882
PstNI CAGNNNCTG 4 cut(s) 100, 150, 670, 2209
PsuI RGATCY 7 cut(s) 96, 1312, 1874, 1937, 2192, 2217, 2564
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 6 cut(s) 43, 331, 839, 1769, 1926, 2291
RsaNI GTAC 6 cut(s) 42, 330, 838, 1768, 1925, 2290
RseI CAYNNNNRTG 2 cut(s) 2415, 2439
Rsr2I CGGWCCG 2 cut(s) 548, 1343
RsrII CGGWCCG 2 cut(s) 548, 1343
SacI GAGCTC 3 cut(s) 384, 822, 1803
SacII CCGCGG 1 cut(s) 2486
SapI GCTCTTC 3 cut(s) 1808, 2612, 2877
SaqAI TTAA 7 cut(s) 308, 653, 1595, 1850, 2057, 2139, 2898
SatI GCNGC 7 cut(s) 29, 163, 506, 1133, 1541, 2358, 2730
SchI GAGTC 4 cut(s) 453, 580, 704, 1329
SduI GDGCHC 9 cut(s) 384, 808, 822, 986, 1803, 1969, 2384, 2436, 2505
SexAI ACCWGGT 1 cut(s) 723
SfaNI GCATC 5 cut(s) 1039, 1150, 1527, 2305, 2540
SfcI CTRYAG 2 cut(s) 878, 2629
SfiI GGCCNNNNNGGCC 1 cut(s) 80
Sfr274I CTCGAG 1 cut(s) 383
Sfr303I CCGCGG 1 cut(s) 2486
SfuI TTCGAA 3 cut(s) 1246, 1318, 1844
SgrAI CRCCGGYG 3 cut(s) 1124, 1556, 1700
SgrBI CCGCGG 1 cut(s) 2486
SinI GGWCC 6 cut(s) 288, 548, 1343, 1587, 1631, 1678
SlaI CTCGAG 1 cut(s) 383
SmaI CCCGGG 1 cut(s) 1830
SmiMI CAYNNNNRTG 2 cut(s) 2415, 2439
SmlI CTYRAG 2 cut(s) 383, 1730
SmoI CTYRAG 2 cut(s) 383, 1730
SspI AATATT 1 cut(s) 703
SspMI CTAG 2 cut(s) 977, 2952
SstI GAGCTC 3 cut(s) 384, 822, 1803
TaiI ACGT 8 cut(s) 69, 875, 1407, 1467, 2373, 2516, 2529, 2628
TaqI TCGA 8 cut(s) 123, 384, 1246, 1318, 1380, 1844, 2466, 2925
TatI WGTACW 2 cut(s) 1924, 2289
TauI GCSGC 2 cut(s) 508, 2360
Tru1I TTAA 7 cut(s) 308, 653, 1595, 1850, 2057, 2139, 2898
Tru9I TTAA 7 cut(s) 308, 653, 1595, 1850, 2057, 2139, 2898
TscAI CASTG 1 cut(s) 2008
TseFI GTSAC 4 cut(s) 894, 1652, 2005, 2522
TseI GCWGC 5 cut(s) 28, 162, 1132, 1540, 2729
Tsp45I GTSAC 4 cut(s) 894, 1652, 2005, 2522
TspDTI ATGAA 8 cut(s) 632, 1275, 1572, 1850, 2049, 2396, 2787, 2834
TspGWI ACGGA 3 cut(s) 540, 918, 1485
TspMI CCCGGG 1 cut(s) 1828
TspRI CASTG 1 cut(s) 2008
Van91I CCANNNNNTGG 2 cut(s) 670, 2395
VneI GTGCAC 2 cut(s) 804, 2432
VpaK11BI GGWCC 6 cut(s) 288, 548, 1343, 1587, 1631, 1678
XagI CCTNNNNNAGG 1 cut(s) 977
XbaI TCTAGA 1 cut(s) 976
XceI RCATGY 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 2 cut(s) 721, 2917
XhoI CTCGAG 1 cut(s) 383
XmaI CCCGGG 1 cut(s) 1828
XmiI GTMKAC 2 cut(s) 1816, 2628
XmnI GAANNNNTTC 5 cut(s) 212, 905, 1190, 1386, 1550
XspI CTAG 2 cut(s) 977, 2952
ZraI GACGTC 3 cut(s) 873, 1465, 2626
Zsp2I ATGCAT 2 cut(s) 1326, 2596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.