RLG00000017742

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014085
Physical Location & Seq
Reverse (-)
20665485 .. 20669223
3739 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017742

Sequence Viewer

Length: 2961 bp
ATGATGACACTTTTAGCTCCAAAACCGCTGCTGATTCTCTGCACCCTATCATTTTACTTCTCCTACGTTATGGCCTTTTCGGCCGGAGACACCCAGATCCTGCTCCGGGTAAAAACCCAACTCGACGACCCGGCTGGAAACCTCAACGACTGGGTTTCCGGCAGCAATCACAGTCCATGCAATTGGTCAGGAATCACTTGTGAGCAGAAAAATTTCTCCATTGTTTCAATAGACATGTCCGGGTTTGGTGTTCACGGCGGATTTCCAGCTGAGTTGTGCCGGATTCGGACCCTTCAGAAACTCACTTTGAACTCCAACTCCATCAACGGTACACTCTCCACTAAGCTCCTGTCTCTCTGCTCACACCTTCAAGCTTTGGAGATCGAGGAGAACGATATCGTCGGAGAATTGCCGGAATTTCTACCCGAGTTTACTGGTTTACGAGTCTTGAATCTCCAAACCAACAACTTTACTGGAGATATCCCAGCGAGTTTCGGGCGGTTGCCGCAGCTGAGGGTGCTGAAGCTTTCCGGGAACTTGCTCACCGGTCCGTTTCCAAAGTTCTTGACCAACCTGAGTGAGTTGACTCATCTGGATTTTGCTTACAATCCAACAATGAAGCCCAGTTTGCTCCCTCCAGAAATCGGAAACTTAACCAATCTCCAGAGGCTGGTTATCTTCAATATCAATCTAAAAGGCAATATTCCGGACTCCATCGGAAACCTGGTCTCGCTCAGAAACCTGGACTTGTCCCAGAATTCGTTATCCGGGACAATTCCAGTGAGCATTGGAAGATTGAGAAGTGCACAACAAATAGAGCTCTATTTGAATCGATTGTACGGCGAATTGCCGGACAGTATTGCAAACTTGACGTCTCTGCAGAACTTGGATTTGTCACAGAACGGATTCACCGGCAAATTTTCTGAAAGAATCGCTGGAATTCGTTTCGTTTCTCTGCGACTGGCCGATAACCTTCTGGAGGGCTCTGTTCCGGAGATCGTAGCCAACAGTCCTGATCTGGTTCAGTTGCATCTCTTCAATAACAGCTTTTCCGGTTCTCTCCCGGAAAATCTCGGCCGAAATTCAGCTCTGCAAGATTTAGATGTCTCAACCAATAAATTCACCGGCGAGCTGCCTCGGCATCTCTGCTACGGTAAAAATCTCAGCAGTCTGGTTATATTCGGGAACCAATTCTCTGGAAGTTTACCGGATACACTAAGCGAGTGTCAATCCCTGAACTATGTTCGAATTGAACAGAATGAACTCTCCGGTCAAGTGTCGGACAAGTTTTGGGGTCTGCCTCTGCTTACACATCTTCGAATGCATTACAACAGACTCAGCGGACCGGTTCCGTCTTCTATTTCCTTTGCTAATGGTCTCGAAACCCTTACAATCTCTGGCAACGTCTTCTCCGGCCAGCTTCCACCGCAGATTTGCAAGTTGTCCAAGCTGGTAGAGCTGGACGTGAGCGACAATCAGTTTTCCGGTGAAGTTCCGTCTTGTATGACAGAGCTGAAGAATCTTCAGAAGCTTAGGATGCAGCAGAATTTGTTCACCGGTGAAATTCCAAGACATCTGAGTTCATGGACCCAGTTAACCGAGTTGAACCTATCCAAAAACCAACTCTCAGGTCCAATTCCTCCTGAGCTGGGTGACTTACCGGTGCTGAATTACTTGGACCTCTCTGATAACTCACTCACCGGTGAGATTCCGGTGGATTTGACCAAGCTCAAGCTCGGCCAGTTCAATCTCTCAGACAACAAACTGTACGGTGAGATACCAACTGGGCTGGACTACGAGCTCTTCGCACCGGGTCTACTGGGCAACCCGGGTCTATGTAGTTCGAATTTAAAGCAGGTTCATCCATGTCAGAGATCCAAATCCCCTACTTTTTTATTGGCTGTTATTCTCTCCATTTGCGTTGTGCTGCTGGTTGGAACTGTTCTTTGGTTTCTGAAAATTAGAGCACAAGTTTTTGGCGGTAAAGCCAAACGGCTTTACAGTGTGACCTCTTTTCAACGGGTCGGGTTCAATGAAGACGATGTGTTACCCTCGTTAACAAATGAGAATCTGATTGCAACGGGCGGGTCGGGTCATGTTTACAGAGTGAAGTTGAAGTCGGGCCAAGTTTTGGCGGTTAAGAAGCTATGGGGTGGAAGCAGCAGGAAGCCGGAAAGTGAGTTGGTTTTTAGATCCGAGGTGGAGACGCTGGGTCGGATCCGGCATGGCAATATTGTAAAATTGGTGTTCTGTTGTAGTGGGGAGGATTGTAGGATATTGGCCTATGAGTACATGGTGAATGGAAGCTTGGGTGATGCTTTGCATGGAGAGAAGCTTGGGCCTTTGATGGACTGGGCCGCGAGGTTCAACGTTGCTTTGGGCTCGGCCCACGGGCTGGCCTATCTTCATCACGACTGTGTTCCGGCGATCGTGCACCGAGATGTGAAGAGTAACAACATATTGCTCGACGAGGAGTGGACACCGCGTGTGGCGGATTTCGGGCTCGCCAAGACGTTGGAGCGTGACGTGGCATCCGGACATGGTGCCATGTCACGGATAGCTGGATCCTACGGCTATATTGCGCCAGAATATGCGTACACACTGAAAGTTACTGAGAAGAGCGACGTCTACAGCTTCGGTGTGGTGTTACTGGAACTGATCACCGGTAAGAGGCCAAATGACTTGTCCTTTGGGGAGAACAAGGACATAGTGAAGTGGGTTACTGAGGCTGCACTGTCTTCTCCTTCCTCTGGTGGGGACGGTGAAAATGCAGAAGTTAGTGGGATTGATCTTAGCCAGATTGTTGACCCGAGAATGAACCCAACTACAAGGGATTTTGAAGAGATTGAGGATGTTTTGAGTGTGGCTCTTCTTTGCACCTCTGAGTTCCCAATTAACAGGCCATCCATGAGAAGAGTGGTCGAATTGCTAAAGGATCAGAAACCAGCTAGTCAAAAATGA

Protein Analysis

987

Amino Acids

108.23

Weight (kDa)

5.79

Isoelectric Point (pI)

34.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 67 4.6e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 94 - 226 5.5e-08 Leucine-rich repeat region
LRR_8 PF13855 146 - 203 1.4e-08 Leucine rich repeat
LRR_14 PF23598 214 - 300 6.1e-09 Leucine-rich repeat region
LRR_14 PF23598 389 - 538 3.3e-09 Leucine-rich repeat region
Pkinase PF00069 689 - 896 1.1e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 689 - 974 1.7e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65710
fragaria_vesca FvH4_1g18670
malus_domestica MD02G1194100.v1.1 MD05G1283300.v1.1 MD15G1304600.v1.1
prunus_persica Prupe.6G212600_v2.0.a1
pyrus_communis pycom02g15810 pycom15g26710
rosa_chinensis RchiOBHm_Chr2g0109521
rosa_laevigata RLG00000017742
rosa_multiflora Rmu_sc0000332.1_g000032
rosa_roxburghii Rroxscaffold_2G00134180
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2AG212900 Rh2BG223100 Rh2CG214700 Rh2DG218500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 875, 2628
Acc36I ACCTGC 1 cut(s) 1846
AccB1I GGYRCC 1 cut(s) 2543
AccB7I CCANNNNNTGG 3 cut(s) 670, 2131, 2395
AccI GTMKAC 2 cut(s) 1816, 2628
AccII CGCG 2 cut(s) 2360, 2485
AccIII TCCGGA 3 cut(s) 706, 991, 2534
AclI AACGTT 1 cut(s) 2370
AclWI GGATC 8 cut(s) 91, 1869, 2187, 2212, 2225, 2559, 2572, 2943
AcoI YGGCCR 5 cut(s) 81, 963, 1075, 1414, 1738
AcuI CTGAAG 4 cut(s) 278, 542, 1508, 1535
AcyI GRCGYC 2 cut(s) 872, 2625
AdeI CACNNNGTG 1 cut(s) 2486
AfaI GTAC 5 cut(s) 331, 839, 1769, 2291, 2597
AflIII ACRYGT 1 cut(s) 234
AgeI ACCGGT 6 cut(s) 545, 1345, 1556, 1660, 1700, 2663
AhdI GACNNNNNGTC 1 cut(s) 2211
AjiI CACGTC 2 cut(s) 1465, 2527
AjnI CCWGG 2 cut(s) 723, 741
AjuI GAANNNNNNNTTGG 4 cut(s) 1930, 1962, 2291, 2323
AleI CACNNNNGTG 1 cut(s) 2415
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
Alw21I GWGCWC 5 cut(s) 808, 822, 1803, 1969, 2436
Alw26I GTCTC 7 cut(s) 81, 357, 733, 879, 1111, 1382, 2198
Alw44I GTGCAC 2 cut(s) 804, 2432
AlwI GGATC 8 cut(s) 91, 1869, 2187, 2212, 2225, 2559, 2572, 2943
AlwNI CAGNNNCTG 2 cut(s) 100, 670
Ama87I CYCGRG 3 cut(s) 425, 1828, 2809
Aor13HI TCCGGA 3 cut(s) 706, 991, 2534
ApaLI GTGCAC 2 cut(s) 804, 2432
ApeKI GCWGC 8 cut(s) 28, 162, 508, 1132, 1540, 1927, 2160, 2729
ArsI GACNNNNNNTTYG 6 cut(s) 857, 889, 2669, 2672, 2701, 2704
AsiGI ACCGGT 6 cut(s) 545, 1345, 1556, 1660, 1700, 2663
Asp700I GAANNNNTTC 4 cut(s) 212, 905, 1190, 1550
AspLEI GCGC 1 cut(s) 2584
AsuC2I CCSGG 9 cut(s) 107, 131, 241, 532, 769, 1064, 1812, 1829, 1830
AsuII TTCGAA 3 cut(s) 1246, 1318, 1844
AvaI CYCGRG 3 cut(s) 425, 1828, 2809
AvaII GGWCC 6 cut(s) 288, 548, 1343, 1587, 1631, 1678
BaeGI GKGCMC 2 cut(s) 808, 2436
BamHI GGATCC 2 cut(s) 2217, 2564
BanI GGYRCC 1 cut(s) 2543
BanII GRGCYC 5 cut(s) 822, 986, 1803, 2384, 2505
BbsI GAAGAC 4 cut(s) 1347, 1399, 2043, 2730
Bbv12I GWGCWC 5 cut(s) 808, 822, 1803, 1969, 2436
BbvCI CCTCAGC 1 cut(s) 512
BbvI GCAGC 8 cut(s) 15, 174, 520, 1119, 1552, 1914, 2172, 2716
BccI CCATC 4 cut(s) 329, 722, 2341, 2911
BceAI ACGGC 4 cut(s) 271, 856, 2009, 2587
BciT130I CCWGG 2 cut(s) 725, 743
BciVI GTATCC 1 cut(s) 1204
BclI TGATCA 1 cut(s) 2658
BcnI CCSGG 9 cut(s) 107, 131, 241, 532, 769, 1064, 1812, 1829, 1830
BcoDI GTCTC 7 cut(s) 81, 357, 733, 879, 1111, 1382, 2198
BfaI CTAG 1 cut(s) 2949
BfmI CTRYAG 2 cut(s) 878, 2629
BfuAI ACCTGC 1 cut(s) 1846
BfuI GTATCC 1 cut(s) 1204
BglI GCCNNNNNGGC 1 cut(s) 80
Bme18I GGWCC 6 cut(s) 288, 548, 1343, 1587, 1631, 1678
BmeRI GACNNNNNGTC 1 cut(s) 2211
BmeT110I CYCGRG 3 cut(s) 425, 1828, 2809
BmgBI CACGTC 2 cut(s) 1465, 2527
BmiI GGNNCC 7 cut(s) 290, 1187, 1350, 1589, 2219, 2545, 2566
BmrI ACTGGG 6 cut(s) 160, 618, 1585, 1794, 1829, 2362
BmsI GCATC 5 cut(s) 1039, 1150, 1527, 2305, 2540
BmuI ACTGGG 6 cut(s) 160, 618, 1585, 1794, 1829, 2362
BpiI GAAGAC 4 cut(s) 1347, 1399, 2043, 2730
BplI GAGNNNNNCTC 2 cut(s) 2851, 2883
BpmI CTGGAG 4 cut(s) 495, 621, 647, 998
Bpu10I CCTNAGC 3 cut(s) 512, 1532, 1644
Bpu14I TTCGAA 3 cut(s) 1246, 1318, 1844
BpuEI CTTGAG 1 cut(s) 1715
BpuMI CCSGG 9 cut(s) 107, 131, 241, 532, 769, 1064, 1812, 1829, 1830
Bsa29I ATCGAT 1 cut(s) 832
BsaBI GATNNNNATC 1 cut(s) 1879
BsaHI GRCGYC 2 cut(s) 872, 2625
BsaI GGTCTC 2 cut(s) 733, 1382
BsaJI CCNNGG 4 cut(s) 1136, 1828, 2196, 2389
BsaXI ACNNNNNCTCC 4 cut(s) 302, 332, 1394, 1424
Bse118I RCCGGY 8 cut(s) 545, 911, 1124, 1345, 1556, 1660, 1700, 2663
Bse8I GATNNNNATC 1 cut(s) 1879
BseAI TCCGGA 3 cut(s) 706, 991, 2534
BseBI CCWGG 2 cut(s) 725, 743
BseCI ATCGAT 1 cut(s) 832
BseDI CCNNGG 4 cut(s) 1136, 1828, 2196, 2389
BseGI GGATG 5 cut(s) 1542, 1861, 2531, 2857, 2903
BseJI GATNNNNATC 1 cut(s) 1879
BseRI GAGGAG 2 cut(s) 401, 2486
BseSI GKGCMC 2 cut(s) 808, 2436
BseX3I CGGCCG 2 cut(s) 81, 1075
BseXI GCAGC 8 cut(s) 15, 174, 520, 1119, 1552, 1914, 2172, 2716
BseYI CCCAGC 3 cut(s) 484, 1648, 2209
BsgI GTGCAG 2 cut(s) 25, 2715
Bsh1236I CGCG 2 cut(s) 2360, 2485
Bsh1285I CGRYCG 3 cut(s) 84, 1078, 2430
BshNI GGYRCC 1 cut(s) 2543
BshTI ACCGGT 6 cut(s) 545, 1345, 1556, 1660, 1700, 2663
BshVI ATCGAT 1 cut(s) 832
BsiEI CGRYCG 3 cut(s) 84, 1078, 2430
BsiHKAI GWGCWC 5 cut(s) 808, 822, 1803, 1969, 2436
BsiHKCI CYCGRG 3 cut(s) 425, 1828, 2809
BslFI GGGAC 3 cut(s) 736, 784, 2771
BsmAI GTCTC 7 cut(s) 81, 357, 733, 879, 1111, 1382, 2198
BsmBI CGTCTC 2 cut(s) 879, 2198
BsmFI GGGAC 3 cut(s) 736, 784, 2771
BsmI GAATGC 1 cut(s) 1326
Bso31I GGTCTC 2 cut(s) 733, 1382
BsoBI CYCGRG 3 cut(s) 425, 1828, 2809
Bsp119I TTCGAA 3 cut(s) 1246, 1318, 1844
Bsp1286I GDGCHC 8 cut(s) 808, 822, 986, 1803, 1969, 2384, 2436, 2505
Bsp13I TCCGGA 3 cut(s) 706, 991, 2534
BspDI ATCGAT 1 cut(s) 832
BspEI TCCGGA 3 cut(s) 706, 991, 2534
BspFNI CGCG 2 cut(s) 2360, 2485
BspLI GGNNCC 7 cut(s) 290, 1187, 1350, 1589, 2219, 2545, 2566
BspMAI CTGCAG 1 cut(s) 882
BspMI ACCTGC 1 cut(s) 1846
BspPI GGATC 8 cut(s) 91, 1869, 2187, 2212, 2225, 2559, 2572, 2943
BspQI GCTCTTC 3 cut(s) 1808, 2612, 2874
BspT104I TTCGAA 3 cut(s) 1246, 1318, 1844
BspT107I GGYRCC 1 cut(s) 2543
BspTNI GGTCTC 2 cut(s) 733, 1382
BsrFI RCCGGY 8 cut(s) 545, 911, 1124, 1345, 1556, 1660, 1700, 2663
BssAI RCCGGY 8 cut(s) 545, 911, 1124, 1345, 1556, 1660, 1700, 2663
BssECI CCNNGG 4 cut(s) 1136, 1828, 2196, 2389
BssNI GRCGYC 2 cut(s) 872, 2625
Bst2UI CCWGG 2 cut(s) 725, 743
Bst6I CTCTTC 7 cut(s) 1040, 1808, 2441, 2612, 2835, 2874, 2908
BstACI GRCGYC 2 cut(s) 872, 2625
BstBI TTCGAA 3 cut(s) 1246, 1318, 1844
BstC8I GCNNGC 4 cut(s) 1130, 1418, 2397, 2505
BstDSI CCRYGG 1 cut(s) 2389
BstENI CCTNNNNNAGG 1 cut(s) 977
BstF5I GGATG 5 cut(s) 1542, 1861, 2531, 2857, 2903
BstFNI CGCG 2 cut(s) 2360, 2485
BstHHI GCGC 1 cut(s) 2584
BstMAI GTCTC 7 cut(s) 81, 357, 733, 879, 1111, 1382, 2198
BstMCI CGRYCG 3 cut(s) 84, 1078, 2430
BstMWI GCNNNNNNNGC 8 cut(s) 80, 505, 517, 628, 1138, 1426, 1456, 1537
BstNI CCWGG 2 cut(s) 725, 743
BstNSI RCATGY 1 cut(s) 238
BstSFI CTRYAG 2 cut(s) 878, 2629
BstSLI GKGCMC 2 cut(s) 808, 2436
BstUI CGCG 2 cut(s) 2360, 2485
BstV1I GCAGC 8 cut(s) 15, 174, 520, 1119, 1552, 1914, 2172, 2716
BstV2I GAAGAC 4 cut(s) 1347, 1399, 2043, 2730
BstX2I RGATCY 5 cut(s) 96, 1874, 2192, 2217, 2564
BstXI CCANNNNNNTGG 3 cut(s) 183, 1196, 2515
BstYI RGATCY 5 cut(s) 96, 1874, 2192, 2217, 2564
BstZI CGGCCG 2 cut(s) 81, 1075
Bsu15I ATCGAT 1 cut(s) 832
BsuI GTATCC 1 cut(s) 1204
BsuTUI ATCGAT 1 cut(s) 832
BtgI CCRYGG 1 cut(s) 2389
BtrI CACGTC 2 cut(s) 1465, 2527
BtsCI GGATG 5 cut(s) 1542, 1861, 2531, 2857, 2903
BtsIMutI CAGTG 4 cut(s) 786, 2008, 2600, 2732
BveI ACCTGC 1 cut(s) 1846
Cac8I GCNNGC 4 cut(s) 1130, 1418, 2397, 2505
CaiI CAGNNNCTG 2 cut(s) 100, 670
CfoI GCGC 1 cut(s) 2584
Cfr10I RCCGGY 8 cut(s) 545, 911, 1124, 1345, 1556, 1660, 1700, 2663
Cfr9I CCCGGG 1 cut(s) 1828
ClaI ATCGAT 1 cut(s) 832
CpoI CGGWCCG 2 cut(s) 548, 1343
CseI GACGC 1 cut(s) 2215
CsiI ACCWGGT 1 cut(s) 723
Csp6I GTAC 5 cut(s) 330, 838, 1768, 2290, 2596
CspAI ACCGGT 6 cut(s) 545, 1345, 1556, 1660, 1700, 2663
CspI CGGWCCG 2 cut(s) 548, 1343
CviQI GTAC 5 cut(s) 330, 838, 1768, 2290, 2596
DraI TTTAAA 1 cut(s) 1851
DraIII CACNNNGTG 1 cut(s) 2486
DriI GACNNNNNGTC 1 cut(s) 2211
EaeI YGGCCR 5 cut(s) 81, 963, 1075, 1414, 1738
EagI CGGCCG 2 cut(s) 81, 1075
Eam1104I CTCTTC 7 cut(s) 1040, 1808, 2441, 2612, 2835, 2874, 2908
Eam1105I GACNNNNNGTC 1 cut(s) 2211
EarI CTCTTC 7 cut(s) 1040, 1808, 2441, 2612, 2835, 2874, 2908
EciI GGCGGA 2 cut(s) 273, 2507
Ecl136II GAGCTC 2 cut(s) 820, 1801
EclXI CGGCCG 2 cut(s) 81, 1075
Eco24I GRGCYC 5 cut(s) 822, 986, 1803, 2384, 2505
Eco31I GGTCTC 2 cut(s) 733, 1382
Eco32I GATATC 2 cut(s) 397, 481
Eco47I GGWCC 6 cut(s) 288, 548, 1343, 1587, 1631, 1678
Eco52I CGGCCG 2 cut(s) 81, 1075
Eco53kI GAGCTC 2 cut(s) 820, 1801
Eco57I CTGAAG 4 cut(s) 278, 542, 1508, 1535
Eco88I CYCGRG 3 cut(s) 425, 1828, 2809
EcoICRI GAGCTC 2 cut(s) 820, 1801
EcoNI CCTNNNNNAGG 1 cut(s) 977
EcoRI GAATTC 2 cut(s) 757, 939
EcoRII CCWGG 2 cut(s) 723, 741
EcoRV GATATC 2 cut(s) 397, 481
EcoT22I ATGCAT 1 cut(s) 1326
EcoT38I GRGCYC 5 cut(s) 822, 986, 1803, 2384, 2505
Esp3I CGTCTC 2 cut(s) 879, 2198
FaqI GGGAC 3 cut(s) 736, 784, 2771
FauI CCCGC 1 cut(s) 2078
FbaI TGATCA 1 cut(s) 2658
FblI GTMKAC 2 cut(s) 1816, 2628
FokI GGATG 5 cut(s) 1549, 1848, 2518, 2864, 2890
FriOI GRGCYC 5 cut(s) 822, 986, 1803, 2384, 2505
FspBI CTAG 1 cut(s) 2949
GlaI GCGC 1 cut(s) 2583
GsaI CCCAGC 3 cut(s) 488, 1652, 2213
GsuI CTGGAG 4 cut(s) 495, 621, 647, 998
HgaI GACGC 1 cut(s) 2215
HhaI GCGC 1 cut(s) 2584
Hin1I GRCGYC 2 cut(s) 872, 2625
Hin6I GCGC 1 cut(s) 2582
HinP1I GCGC 1 cut(s) 2582
HincII GTYRAC 4 cut(s) 585, 1596, 2058, 2806
HindII GTYRAC 4 cut(s) 585, 1596, 2058, 2806
HindIII AAGCTT 5 cut(s) 372, 524, 1529, 2305, 2333
HpaI GTTAAC 2 cut(s) 1596, 2058
Hpy99I CGWCG 4 cut(s) 128, 404, 2471, 2627
HpyAV CCTTC 4 cut(s) 302, 377, 983, 2754
HpyCH4IV ACGT 8 cut(s) 66, 872, 1404, 1464, 2370, 2513, 2526, 2625
HpyF10VI GCNNNNNNNGC 8 cut(s) 80, 505, 517, 628, 1138, 1426, 1456, 1537
HpySE526I ACGT 8 cut(s) 66, 872, 1404, 1464, 2370, 2513, 2526, 2625
Hsp92I GRCGYC 2 cut(s) 872, 2625
HspAI GCGC 1 cut(s) 2582
Kpn2I TCCGGA 3 cut(s) 706, 991, 2534
Ksp22I TGATCA 1 cut(s) 2658
KspAI GTTAAC 2 cut(s) 1596, 2058
LguI GCTCTTC 3 cut(s) 1808, 2612, 2874
LmnI GCTCC 5 cut(s) 22, 108, 351, 636, 2518
Lsp1109I GCAGC 8 cut(s) 15, 174, 520, 1119, 1552, 1914, 2172, 2716
LweI GCATC 5 cut(s) 1039, 1150, 1527, 2305, 2540
MabI ACCWGGT 1 cut(s) 723
MaeI CTAG 1 cut(s) 2949
MaeII ACGT 8 cut(s) 66, 872, 1404, 1464, 2370, 2513, 2526, 2625
MfeI CAATTG 1 cut(s) 181
MflI RGATCY 5 cut(s) 96, 1874, 2192, 2217, 2564
MhlI GDGCHC 8 cut(s) 808, 822, 986, 1803, 1969, 2384, 2436, 2505
MlyI GAGTC 4 cut(s) 453, 580, 704, 1329
MmeI TCCRAC 7 cut(s) 339, 382, 635, 1260, 1915, 2195, 2496
Mph1103I ATGCAT 1 cut(s) 1326
MroI TCCGGA 3 cut(s) 706, 991, 2534
MroXI GAANNNNTTC 4 cut(s) 212, 905, 1190, 1550
MseI TTAA 6 cut(s) 653, 1595, 1850, 2057, 2139, 2895
MslI CAYNNNNRTG 2 cut(s) 2415, 2439
MspA1I CMGCKG 4 cut(s) 28, 269, 511, 1341
MunI CAATTG 1 cut(s) 181
Mva1269I GAATGC 1 cut(s) 1326
MvaI CCWGG 2 cut(s) 725, 743
MvnI CGCG 2 cut(s) 2360, 2485
MwoI GCNNNNNNNGC 8 cut(s) 80, 505, 517, 628, 1138, 1426, 1456, 1537
NciI CCSGG 9 cut(s) 107, 131, 241, 532, 769, 1064, 1812, 1829, 1830
NlaIV GGNNCC 7 cut(s) 290, 1187, 1350, 1589, 2219, 2545, 2566
NmeAIII GCCGAG 4 cut(s) 1053, 1117, 1716, 2363
NmuCI GTSAC 5 cut(s) 894, 1652, 2005, 2522, 2550
NsiI ATGCAT 1 cut(s) 1326
NspI RCATGY 1 cut(s) 238
NspV TTCGAA 3 cut(s) 1246, 1318, 1844
OliI CACNNNNGTG 1 cut(s) 2415
PciI ACATGT 1 cut(s) 234
PciSI GCTCTTC 3 cut(s) 1808, 2612, 2874
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PctI GAATGC 1 cut(s) 1326
PdmI GAANNNNTTC 4 cut(s) 212, 905, 1190, 1550
PflMI CCANNNNNTGG 3 cut(s) 670, 2131, 2395
PfoI TCCNGGA 3 cut(s) 530, 767, 1062
PinAI ACCGGT 6 cut(s) 545, 1345, 1556, 1660, 1700, 2663
Ple19I CGATCG 1 cut(s) 2430
PleI GAGTC 4 cut(s) 452, 580, 704, 1329
PpsI GAGTC 4 cut(s) 452, 580, 704, 1329
PscI ACATGT 1 cut(s) 234
Psp124BI GAGCTC 2 cut(s) 822, 1803
Psp1406I AACGTT 1 cut(s) 2370
Psp6I CCWGG 2 cut(s) 723, 741
PspFI CCCAGC 3 cut(s) 484, 1648, 2209
PspGI CCWGG 2 cut(s) 723, 741
PspN4I GGNNCC 7 cut(s) 290, 1187, 1350, 1589, 2219, 2545, 2566
PstI CTGCAG 1 cut(s) 882
PstNI CAGNNNCTG 2 cut(s) 100, 670
PsuI RGATCY 5 cut(s) 96, 1874, 2192, 2217, 2564
PvuI CGATCG 1 cut(s) 2430
PvuII CAGCTG 2 cut(s) 269, 511
RsaI GTAC 5 cut(s) 331, 839, 1769, 2291, 2597
RsaNI GTAC 5 cut(s) 330, 838, 1768, 2290, 2596
RseI CAYNNNNRTG 2 cut(s) 2415, 2439
Rsr2I CGGWCCG 2 cut(s) 548, 1343
RsrII CGGWCCG 2 cut(s) 548, 1343
SacI GAGCTC 2 cut(s) 822, 1803
SapI GCTCTTC 3 cut(s) 1808, 2612, 2874
SaqAI TTAA 6 cut(s) 653, 1595, 1850, 2057, 2139, 2895
SchI GAGTC 4 cut(s) 453, 580, 704, 1329
SduI GDGCHC 8 cut(s) 808, 822, 986, 1803, 1969, 2384, 2436, 2505
SexAI ACCWGGT 1 cut(s) 723
SfaNI GCATC 5 cut(s) 1039, 1150, 1527, 2305, 2540
SfcI CTRYAG 2 cut(s) 878, 2629
SfiI GGCCNNNNNGGCC 1 cut(s) 80
SfuI TTCGAA 3 cut(s) 1246, 1318, 1844
SgrAI CRCCGGYG 3 cut(s) 1124, 1556, 1700
SinI GGWCC 6 cut(s) 288, 548, 1343, 1587, 1631, 1678
SmaI CCCGGG 1 cut(s) 1830
SmiMI CAYNNNNRTG 2 cut(s) 2415, 2439
SmlI CTYRAG 1 cut(s) 1730
SmoI CTYRAG 1 cut(s) 1730
SspI AATATT 2 cut(s) 703, 2233
SspMI CTAG 1 cut(s) 2949
SstI GAGCTC 2 cut(s) 822, 1803
TaiI ACGT 8 cut(s) 69, 875, 1407, 1467, 2373, 2516, 2529, 2628
TaqI TCGA 9 cut(s) 123, 384, 832, 1246, 1318, 1380, 1844, 2466, 2922
TatI WGTACW 1 cut(s) 2289
TauI GCSGC 2 cut(s) 508, 2360
Tru1I TTAA 6 cut(s) 653, 1595, 1850, 2057, 2139, 2895
Tru9I TTAA 6 cut(s) 653, 1595, 1850, 2057, 2139, 2895
TscAI CASTG 4 cut(s) 786, 2008, 2607, 2739
TseFI GTSAC 5 cut(s) 894, 1652, 2005, 2522, 2550
TseI GCWGC 8 cut(s) 28, 162, 508, 1132, 1540, 1927, 2160, 2729
Tsp45I GTSAC 5 cut(s) 894, 1652, 2005, 2522, 2550
TspDTI ATGAA 7 cut(s) 632, 1275, 1572, 1850, 2049, 2396, 2831
TspGWI ACGGA 5 cut(s) 540, 918, 1341, 1485, 2569
TspMI CCCGGG 1 cut(s) 1828
TspRI CASTG 4 cut(s) 786, 2008, 2607, 2739
Van91I CCANNNNNTGG 3 cut(s) 670, 2131, 2395
VneI GTGCAC 2 cut(s) 804, 2432
VpaK11BI GGWCC 6 cut(s) 288, 548, 1343, 1587, 1631, 1678
XagI CCTNNNNNAGG 1 cut(s) 977
XceI RCATGY 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 2 cut(s) 721, 2914
XmaI CCCGGG 1 cut(s) 1828
XmiI GTMKAC 2 cut(s) 1816, 2628
XmnI GAANNNNTTC 4 cut(s) 212, 905, 1190, 1550
XspI CTAG 1 cut(s) 2949
ZraI GACGTC 2 cut(s) 873, 2626
Zsp2I ATGCAT 1 cut(s) 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.