MD05G1283300.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014085
Physical Location & Seq
Reverse (-)
41683859 .. 41684554
696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1283300.v1.1.491

Sequence Viewer

Length: 696 bp
ATGGCGAACCATTTCTCCGAAAATTTACCCGATACGTTAAACGAGTGTCATTCTCTCGGATACGTTCGAATCGAATTCAATGAGCTCGACGGCGTCGTTTCAGACAAGTTTTGGGGTCTGCCTGGTCTCTATTTCGTCACGATGAATAACAACAGATTCAACGGCAGCGTATCGCCGTCGATATCGGCGGCTACTAAATTATCAACACTTAAGATCTCCAGCAATTCATTTTCTGGCGAGCTTCCGTCGGCGATTTGTAAGCTTACGAACCTCTCGAGCCTCGACGTGAGCAGAAACAAATTCTCCAGCGACTTGCCGCCGCGTATAACGGAGCTGAAGATTTTGCAGAGGCTGAAAATGGAGGAGAACATGTTCTCCGGTGAAATTCCGAGTGCAGTGAGTTCGTGGACCCAGTCGACGGAGCTGAGCCTATCTCATAATCGCATAACGGGTCGGATCCCACCCCAACTCGGTTACTTGCCGGTATTGAATTACTTGGATCTCTCAGAAAATTTACTAACCGGTGAGATTCCGTCGGAGTTGACCAAGCTGAGGCTCAATCAGTTCAACGTCTCGGATAATAAACTGTACGGGAAAATACCTACTGGTTTCGATTGCAGTCCCTTCAACTTGGGGCTAGTGGGTAACCCGAATTTGTGCAGTCCGGAGCCATTGTCGGTGCGGCAACGTAGCTGA

Protein Analysis

232

Amino Acids

25.56

Weight (kDa)

5.95

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 66 - 122 2.3e-06 Leucine-rich repeat region
LRR_8 PF13855 140 - 196 7.5e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65710
fragaria_vesca FvH4_1g18670
malus_domestica MD02G1194100.v1.1 MD05G1283300.v1.1 MD15G1304600.v1.1
prunus_persica Prupe.6G212600_v2.0.a1
pyrus_communis pycom02g15810 pycom15g26710
rosa_chinensis RchiOBHm_Chr2g0109521
rosa_laevigata RLG00000017742
rosa_multiflora Rmu_sc0000332.1_g000032
rosa_roxburghii Rroxscaffold_2G00134180
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2AG212900 Rh2BG223100 Rh2CG214700 Rh2DG218500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 416
AccII CGCG 1 cut(s) 322
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 4 cut(s) 188, 317, 320, 682
AclWI GGATC 3 cut(s) 451, 464, 507
AcsI RAATTY 6 cut(s) 22, 74, 299, 384, 511, 652
AcuI CTGAAG 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 1 cut(s) 590
AfiI CCNNNNNNNGG 3 cut(s) 418, 470, 552
AflII CTTAAG 1 cut(s) 209
AflIII ACRYGT 1 cut(s) 369
AgeI ACCGGT 1 cut(s) 521
AgsI TTSAA 5 cut(s) 79, 160, 490, 568, 628
AjiI CACGTC 1 cut(s) 286
AjnI CCWGG 1 cut(s) 121
AloI GAACNNNNNNTCC 5 cut(s) 31, 356, 359, 388, 391
AluBI AGCT 7 cut(s) 85, 241, 262, 334, 424, 550, 693
AluI AGCT 7 cut(s) 85, 241, 262, 334, 424, 550, 693
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 2 cut(s) 131, 577
AlwI GGATC 3 cut(s) 451, 464, 507
AlwNI CAGNNNCTG 1 cut(s) 352
Ama87I CYCGRG 1 cut(s) 274
Aor13HI TCCGGA 1 cut(s) 664
ApeKI GCWGC 1 cut(s) 165
ApoI RAATTY 6 cut(s) 22, 74, 299, 384, 511, 652
AsiGI ACCGGT 1 cut(s) 521
Asp700I GAANNNNTTC 2 cut(s) 11, 371
AspS9I GGNCC 1 cut(s) 408
AsuHPI GGTGA 2 cut(s) 392, 536
AsuII TTCGAA 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 274
AvaII GGWCC 1 cut(s) 408
BamHI GGATCC 1 cut(s) 456
BanII GRGCYC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 87
BbvCI CCTCAGC 1 cut(s) 551
BbvI GCAGC 1 cut(s) 177
BceAI ACGGC 3 cut(s) 106, 160, 178
BcgI CGANNNNNNTGC 2 cut(s) 384, 418
BciT130I CCWGG 1 cut(s) 123
BciVI GTATCC 1 cut(s) 53
BcoDI GTCTC 2 cut(s) 131, 577
BfaI CTAG 1 cut(s) 638
BfrI CTTAAG 1 cut(s) 209
BfuI GTATCC 1 cut(s) 53
BglII AGATCT 1 cut(s) 213
BisI GCNGC 5 cut(s) 166, 189, 317, 320, 683
BlpI GCTNAGC 1 cut(s) 425
BlsI GCNGC 5 cut(s) 167, 190, 318, 321, 684
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 408
BmeT110I CYCGRG 1 cut(s) 274
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 408
BmiI GGNNCC 3 cut(s) 410, 458, 669
BmrFI CCNGG 1 cut(s) 123
BmrI ACTGGG 1 cut(s) 406
BmuI ACTGGG 1 cut(s) 406
BplI GAGNNNNNCTC 2 cut(s) 418, 450
BpmI CTGGAG 2 cut(s) 202, 289
Bpu10I CCTNAGC 1 cut(s) 551
Bpu1102I GCTNAGC 1 cut(s) 425
Bpu14I TTCGAA 1 cut(s) 67
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 131
BsaWI WCCGGW 3 cut(s) 377, 521, 664
BsaXI ACNNNNNCTCC 9 cut(s) 29, 287, 317, 356, 359, 386, 389, 659, 689
Bsc4I CCNNNNNNNGG 3 cut(s) 418, 470, 552
Bse118I RCCGGY 2 cut(s) 481, 521
Bse1I ACTGG 2 cut(s) 412, 610
BseAI TCCGGA 1 cut(s) 664
BseBI CCWGG 1 cut(s) 123
BseLI CCNNNNNNNGG 3 cut(s) 418, 470, 552
BseMII CTCAG 3 cut(s) 416, 519, 542
BseNI ACTGG 2 cut(s) 412, 610
BseRI GAGGAG 1 cut(s) 377
BseXI GCAGC 1 cut(s) 177
BsgI GTGCAG 2 cut(s) 414, 679
Bsh1236I CGCG 1 cut(s) 322
BshTI ACCGGT 1 cut(s) 521
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 274
BsiSI CCGG 4 cut(s) 378, 482, 522, 665
BslFI GGGAC 1 cut(s) 606
BslI CCNNNNNNNGG 3 cut(s) 418, 470, 552
BsmAI GTCTC 2 cut(s) 131, 577
BsmBI CGTCTC 1 cut(s) 577
BsmFI GGGAC 1 cut(s) 606
Bso31I GGTCTC 1 cut(s) 131
BsoBI CYCGRG 1 cut(s) 274
Bsp119I TTCGAA 1 cut(s) 67
Bsp1286I GDGCHC 1 cut(s) 87
Bsp13I TCCGGA 1 cut(s) 664
Bsp143I GATC 3 cut(s) 213, 456, 499
Bsp1720I GCTNAGC 1 cut(s) 425
BspACI CCGC 4 cut(s) 188, 317, 320, 682
BspCNI CTCAG 3 cut(s) 417, 518, 543
BspEI TCCGGA 1 cut(s) 664
BspFNI CGCG 1 cut(s) 322
BspLI GGNNCC 3 cut(s) 410, 458, 669
BspPI GGATC 3 cut(s) 451, 464, 507
BspT104I TTCGAA 1 cut(s) 67
BspTI CTTAAG 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 131
BsrFI RCCGGY 2 cut(s) 481, 521
BsrI ACTGG 2 cut(s) 412, 610
BssAI RCCGGY 2 cut(s) 481, 521
BssMI GATC 3 cut(s) 213, 456, 499
BssNI GRCGYC 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 588
BstACI GRCGYC 1 cut(s) 93
BstAFI CTTAAG 1 cut(s) 209
BstBI TTCGAA 1 cut(s) 67
BstC8I GCNNGC 1 cut(s) 239
BstDEI CTNAG 3 cut(s) 425, 505, 551
BstEII GGTNACC 1 cut(s) 644
BstFNI CGCG 1 cut(s) 322
BstKTI GATC 3 cut(s) 216, 459, 502
BstMAI GTCTC 2 cut(s) 131, 577
BstMBI GATC 3 cut(s) 213, 456, 499
BstNI CCWGG 1 cut(s) 123
BstNSI RCATGY 1 cut(s) 373
BstPI GGTNACC 1 cut(s) 644
BstSCI CCNGG 1 cut(s) 121
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 177
BstX2I RGATCY 3 cut(s) 213, 456, 499
BstYI RGATCY 3 cut(s) 213, 456, 499
BsuI GTATCC 1 cut(s) 53
BtrI CACGTC 1 cut(s) 286
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 1 cut(s) 239
CaiI CAGNNNCTG 1 cut(s) 352
Cfr10I RCCGGY 2 cut(s) 481, 521
Cfr13I GGNCC 1 cut(s) 408
CseI GACGC 1 cut(s) 82
Csp6I GTAC 1 cut(s) 589
CspAI ACCGGT 1 cut(s) 521
CviAII CATG 1 cut(s) 370
CviQI GTAC 1 cut(s) 589
DdeI CTNAG 3 cut(s) 425, 505, 551
DpnI GATC 3 cut(s) 215, 458, 501
DpnII GATC 3 cut(s) 213, 456, 499
Ecl136II GAGCTC 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 87
Eco31I GGTCTC 1 cut(s) 131
Eco32I GATATC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 408
Eco53kI GAGCTC 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 356
Eco88I CYCGRG 1 cut(s) 274
Eco91I GGTNACC 1 cut(s) 644
EcoICRI GAGCTC 1 cut(s) 85
EcoO65I GGTNACC 1 cut(s) 644
EcoRI GAATTC 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 121
EcoRV GATATC 1 cut(s) 183
EcoT38I GRGCYC 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 577
FaeI CATG 1 cut(s) 373
FaiI YATR 4 cut(s) 326, 371, 438, 446
FaqI GGGAC 1 cut(s) 606
FatI CATG 1 cut(s) 369
FblI GTMKAC 1 cut(s) 416
Fnu4HI GCNGC 5 cut(s) 166, 189, 317, 320, 683
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 5 cut(s) 166, 189, 317, 320, 683
FspBI CTAG 1 cut(s) 638
GluI GCNGC 5 cut(s) 166, 189, 317, 320, 683
GsuI CTGGAG 2 cut(s) 202, 289
HapII CCGG 4 cut(s) 378, 482, 522, 665
HgaI GACGC 1 cut(s) 82
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 1 cut(s) 373
HincII GTYRAC 2 cut(s) 417, 543
HindII GTYRAC 2 cut(s) 417, 543
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 3 cut(s) 69, 156, 529
HpaII CCGG 4 cut(s) 378, 482, 522, 665
HphI GGTGA 2 cut(s) 392, 536
Hpy166II GTNNAC 3 cut(s) 408, 417, 543
Hpy188I TCNGA 8 cut(s) 19, 59, 103, 390, 456, 508, 538, 577
Hpy188III TCNNGA 3 cut(s) 139, 274, 665
Hpy8I GTNNAC 3 cut(s) 408, 417, 543
Hpy99I CGWCG 7 cut(s) 92, 98, 181, 250, 287, 421, 538
HpyAV CCTTC 1 cut(s) 634
HpyCH4III ACNGT 1 cut(s) 588
HpyCH4IV ACGT 5 cut(s) 35, 63, 285, 570, 688
HpyCH4V TGCA 4 cut(s) 346, 395, 618, 660
HpyF3I CTNAG 3 cut(s) 425, 505, 551
HpySE526I ACGT 5 cut(s) 35, 63, 285, 570, 688
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 1 cut(s) 373
Kpn2I TCCGGA 1 cut(s) 664
Kzo9I GATC 3 cut(s) 213, 456, 499
LmnI GCTCC 3 cut(s) 331, 421, 667
Lsp1109I GCAGC 1 cut(s) 177
MaeI CTAG 1 cut(s) 638
MaeII ACGT 5 cut(s) 35, 63, 285, 570, 688
MaeIII GTNAC 3 cut(s) 136, 473, 644
MalI GATC 3 cut(s) 215, 458, 501
MboI GATC 3 cut(s) 213, 456, 499
MboII GAAGA 1 cut(s) 349
MflI RGATCY 3 cut(s) 213, 456, 499
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 9 cut(s) 22, 74, 197, 223, 299, 384, 490, 511, 652
MmeI TCCRAC 2 cut(s) 434, 516
MnlI CCTC 5 cut(s) 281, 290, 342, 355, 546
MroI TCCGGA 1 cut(s) 664
MroXI GAANNNNTTC 2 cut(s) 11, 371
MseI TTAA 2 cut(s) 38, 210
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 4 cut(s) 378, 482, 522, 665
MspR9I CCNGG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 123
MvnI CGCG 1 cut(s) 322
NdeII GATC 3 cut(s) 213, 456, 499
NlaIII CATG 1 cut(s) 373
NlaIV GGNNCC 3 cut(s) 410, 458, 669
NmuCI GTSAC 1 cut(s) 136
NspI RCATGY 1 cut(s) 373
NspV TTCGAA 1 cut(s) 67
PaeR7I CTCGAG 1 cut(s) 274
PciI ACATGT 1 cut(s) 369
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 93, 272
PdmI GAANNNNTTC 2 cut(s) 11, 371
PfeI GAWTC 3 cut(s) 69, 156, 529
PflFI GACNNNGTC 2 cut(s) 92, 412
PinAI ACCGGT 1 cut(s) 521
PkrI GCNGC 5 cut(s) 167, 190, 318, 321, 684
PscI ACATGT 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 121
PspEI GGTNACC 1 cut(s) 644
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 3 cut(s) 410, 458, 669
PspPI GGNCC 1 cut(s) 408
PstNI CAGNNNCTG 1 cut(s) 352
PsuI RGATCY 3 cut(s) 213, 456, 499
PsyI GACNNNGTC 2 cut(s) 92, 412
RsaI GTAC 1 cut(s) 590
RsaNI GTAC 1 cut(s) 589
SacI GAGCTC 1 cut(s) 87
SalI GTCGAC 1 cut(s) 415
SaqAI TTAA 2 cut(s) 38, 210
SatI GCNGC 5 cut(s) 166, 189, 317, 320, 683
Sau3AI GATC 3 cut(s) 213, 456, 499
Sau96I GGNCC 1 cut(s) 408
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 87
Sfr274I CTCGAG 1 cut(s) 274
SfuI TTCGAA 1 cut(s) 67
SinI GGWCC 1 cut(s) 408
SlaI CTCGAG 1 cut(s) 274
SmlI CTYRAG 2 cut(s) 209, 274
SmoI CTYRAG 2 cut(s) 209, 274
Sse9I AATT 9 cut(s) 22, 74, 197, 223, 299, 384, 490, 511, 652
SsiI CCGC 4 cut(s) 188, 317, 320, 682
SspMI CTAG 1 cut(s) 638
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 121
TaaI ACNGT 1 cut(s) 588
TaiI ACGT 5 cut(s) 38, 66, 288, 573, 691
TaqI TCGA 8 cut(s) 67, 72, 87, 179, 275, 282, 416, 612
TasI AATT 9 cut(s) 22, 74, 197, 223, 299, 384, 490, 511, 652
TauI GCSGC 4 cut(s) 191, 319, 322, 685
TfiI GAWTC 3 cut(s) 69, 156, 529
Tru1I TTAA 2 cut(s) 38, 210
Tru9I TTAA 2 cut(s) 38, 210
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 1 cut(s) 165
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 2 cut(s) 158, 216
TspGWI ACGGA 4 cut(s) 234, 344, 434, 522
TspRI CASTG 1 cut(s) 402
Tth111I GACNNNGTC 2 cut(s) 92, 412
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 408
XapI RAATTY 6 cut(s) 22, 74, 299, 384, 511, 652
XceI RCATGY 1 cut(s) 373
XhoI CTCGAG 1 cut(s) 274
XmiI GTMKAC 1 cut(s) 416
XmnI GAANNNNTTC 2 cut(s) 11, 371
XspI CTAG 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.