MD15G1304600.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014085
Physical Location & Seq
Reverse (-)
29636424 .. 29639880
3457 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1304600.v1.1.491

Sequence Viewer

Length: 2970 bp
ATGACACAATTAACACCCCTAATGCCCCTTTTAGTATTGTTTTCTGCACTGTCATGCATTTTAACAGTCGCAGCATCTTTGGCCGGGGATACTCAGAACTTGATTCGGGTCAAGGCACAACTGTCCGACCCGGACGGAAAGCTCGACGACTGGGTTCCGGGCAGCGGCCACAATCCATGCAATTGGACGGGAATCACCTGCGACCGGGACAACCACTCTGTGCTTGCTGTAAACTTATCCGTGTTCGGTGTTTCGGGCGGGTTCCCGTTCGGGTTTTGTAGCATTCGGACTCTCCAGAACCTCTCTCTGTCCGCCAACTTAATCAACGGCTCTCTCCAAACCAACACCCTCTCTCTCTGCTCCCACCTCCATGTTCTCGAGCTCCACGATAACCTCTTAGTCGGAACATTGCCGGAGTTTCAGCCGGAGTTCACCGACTTGCGAATCCTGAACCTCATAAACAACAACTTCACCGGCGATATCCCGGCGAGTTTCGGCCGGTTTCCTTCCCTGCAAGTCCTGATGCTCTCCGGGAACTTGCTCACCGGTACAATCCCCAAGTTCTTGGGCAATCTTACCGAGTTGACTCGCTTGGAACTCGCTATCAATCCATTGAAGCTCGGCGAGTTGCCTTCCGAAATTGGAAACTTGACGAAGCTACAGACCCTCTTCGCCCCGCAATGCAACATCAGAGGCCGCTTACCAGACTCCATCGGAAACCTCGTCTCGCTGATGAACCTCGATTTGTCTACGAATTTCCTATCCGGTCCGATTCCGGCAAGCATTGGTGGATTGAGGAGCGTGACGCAGATCGAGCTGTACGAAAATCACCTGTTCGGCGAATTGCCTGAGAGCATTGGAAACCTGACCTCTCTCCGGAACTTAGATCTCTCTCTGAACGCCTTCACCGGAAAAATCCCAGAAACAATTGCAGGACTGCAATTGGAGTCTTTGAATCTCAACGACAATCTCCTCTCCGGCGAGCTTCCTCCGATCTTGGCCTCCAATCCGAAACTGCGGCAGTTCAAGATCTTCAACAACAGCTTCTCCGGTTCCCTCCCGGAAAATCTCGGCCGATATTCCGATTTGGCAGAAGTCGACGTCTCGACGAACACGTTTTCCGGAGAGCTGCCGAAGTTTCTCTGTTACCAGAAAAAGCTCTGGAATTTCATCGCAATGGCGAACCATTTTTCCGGAAATTTACCCGATACGTTAAACGAGTGTCATTCTCTCGGATACGTTCGAATCGAATTCAATGAGCTCGACGGCGTCGTTTCAGACAAGTTTTGGGGTCTGACTGGTCTCTATTTCGTCACTATGAATAACAACAGATTCAACGGCAGCGTATCGCCGTCGATATCGGCGGCTACTAAATTCTCGACACTTAAGATCTCCAGCAATTCATTTTCTGGCGAGCTTCCGTCGGCGATTTGTAAGCTTACGAACCTCTCGAGCCTCGACGTGAGCAGAAACAAATTCTCCGGCGACTTGCCGCCGTGCATAACGGAGCTGAAGATTTTGCAGAGGCTGAAAATGGAGGAGAACATGTTCTCCGGTGAAATTCCGAGTGCAGTGAGTTCGTGGACCCAGTTGACGGAGCTGAACCTATCTCACAATGGCATAACGGGTCGGATCCCACCCCAACTCGGTTACTTGCCGGTATTGAATTACTTGGATCTCTCGGAAAATATACTCACCGGTGAGATTCCGTCGGAGTTGACCAAGCTGAGGCTCAATCAGTTCAACGTCTCGAATAATAAACTGTACGGGAAAATACCTGCTGGTTTCGATTGCAGTCCCTTCAACTTGGGGCTAGTGGGTAACCCGAATTTGTGCAGTCCGGATCTGGATCACTTCCCTACCTGCTCCAAACCCAGATCTCCGACCCCAATTTTGATAGCCATTTTATCCGTGTGCGTTGTACTTGTCCTCGGGTCGGTTCTTTCGTATTTGAAATTCCGGTCCAAAGCTTTTGGCGGTAAAACGAAGCGGCTTTGCAGGGTTACCACTTTCCAACGGGTCGGGTTTAATGAAGAGGAGGTGATGCGATCTTTAACGAAGGAGAATCTAATCGCGACGGGCGGTTCGGGTCATGTTTATAAAGTTAAGCTGAAGACGGGGCAGACCGTGGCGGTTAAGAAGCTATGGGGCGGGGCAAGGAAACCTGAAACTGAATCCGTTTTCCTGTCTGAGGTGGGGACTTTGGGTCGGATCCGACATGGCAATATTGTAAAATTGGTGTTTTGTTGTAGTGGTGAGGACAGTAGGATTTTGGGATATGAATACATGGAAAATGGGAGTTTGGGTGACTGTTTGCATGCTGAGAAGGTTGGGCCTTTGGCGGATTGGGCCAAACGGTACGACATTGCGGTCGGGTCAGCCCATGGGTTAGCCTATCTACACCACGACTGCGCTCCGGCAATCGTCCACCGGGACGTGAAGAGTAACAACATACTATTGGACAAGGACTGGACCCCGCTTGTGGCAGATTTTGGACTGGCCAAGACTTTGCACCCTGACATGGGGGCCGGAGGTGGTGCGATGTCACGGATTGCTGGGTCCTACGGCTATATCGCTCCAGAGTATGCATACACGCTTCAAGTAACTGAGAAGAGCGACGTCTACAGCTTCGGTGTGGTGCTGCTGGAACTGATTACTGGCAAGAGTCCGAATGACTCGTCCTTTGGTGAGAACACAGACATTGTGAAGTGGGTTAGAGAGGCTGCAATGTCTTCTCCTGAAGGAGGAGAAGGCAGCAATGACGAAGGTTTCTGCGGCACCAACCTCTGCCAGATAATCGATCCAAGGATTGATCCGTCAACATGCAACTACGAAGAGATTCAGAAGGTTTTGAAGGTGGCTCTTCTTTGCACTGCTGCATTCCCAATTAACAGGCCCTCCATGAGAAGGGTGGTCGAATTGCTTCGAGATCAGAAACCATCTCGACCGTCCAAAATGATAAGCCTTTGA

Protein Analysis

990

Amino Acids

107.79

Weight (kDa)

6.11

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 68 9.8e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 147 - 205 3.1e-07 Leucine rich repeat
LRR_14 PF23598 204 - 357 2.8e-13 Leucine-rich repeat region
LRR_14 PF23598 470 - 537 4.7e-06 Leucine-rich repeat region
Pkinase PF00069 687 - 897 6.9e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 687 - 907 1.5e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65710
fragaria_vesca FvH4_1g18670
malus_domestica MD02G1194100.v1.1 MD05G1283300.v1.1 MD15G1304600.v1.1
prunus_persica Prupe.6G212600_v2.0.a1
pyrus_communis pycom02g15810 pycom15g26710
rosa_chinensis RchiOBHm_Chr2g0109521
rosa_laevigata RLG00000017742
rosa_multiflora Rmu_sc0000332.1_g000032
rosa_roxburghii Rroxscaffold_2G00134180
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2AG212900 Rh2BG223100 Rh2CG214700 Rh2DG218500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2100
AarI CACCTGC 1 cut(s) 206
AasI GACNNNNNNGTC 1 cut(s) 2369
AatII GACGTC 2 cut(s) 1104, 2622
Acc36I ACCTGC 3 cut(s) 206, 1786, 1871
AccB1I GGYRCC 1 cut(s) 2777
AccI GTMKAC 3 cut(s) 749, 1098, 2622
AccII CGCG 1 cut(s) 2075
AccIII TCCGGA 4 cut(s) 876, 1121, 1193, 1840
AclWI GGATC 9 cut(s) 1627, 1640, 1683, 1851, 1857, 2206, 2219, 2795, 2807
AcoI YGGCCR 5 cut(s) 81, 166, 496, 1072, 2499
AcsI RAATTY 9 cut(s) 754, 1165, 1198, 1250, 1373, 1475, 1560, 1828, 1955
AcuI CTGAAG 3 cut(s) 1532, 2132, 2760
AcyI GRCGYC 3 cut(s) 1101, 1269, 2619
AdeI CACNNNGTG 1 cut(s) 220
AfaI GTAC 5 cut(s) 550, 821, 1766, 1923, 2360
AflII CTTAAG 1 cut(s) 1385
AflIII ACRYGT 2 cut(s) 1113, 1545
AgeI ACCGGT 2 cut(s) 545, 1697
AhdI GACNNNNNGTC 1 cut(s) 2205
AjiI CACGTC 2 cut(s) 1462, 2437
AloI GAACNNNNNNTCC 8 cut(s) 1103, 1135, 1175, 1207, 1532, 1535, 1564, 1567
Alw21I GWGCWC 2 cut(s) 384, 1263
Alw26I GTCTC 4 cut(s) 730, 1108, 1307, 1753
AlwI GGATC 9 cut(s) 1627, 1640, 1683, 1851, 1857, 2206, 2219, 2795, 2807
AlwNI CAGNNNCTG 1 cut(s) 1528
Ama87I CYCGRG 3 cut(s) 377, 1450, 1931
Aor13HI TCCGGA 4 cut(s) 876, 1121, 1193, 1840
ApeKI GCWGC 8 cut(s) 71, 162, 1129, 1341, 2641, 2723, 2754, 2876
ApoI RAATTY 9 cut(s) 754, 1165, 1198, 1250, 1373, 1475, 1560, 1828, 1955
ArsI GACNNNNNNTTYG 8 cut(s) 1876, 1908, 2068, 2100, 2663, 2666, 2695, 2698
AsiGI ACCGGT 2 cut(s) 545, 1697
Asp700I GAANNNNTTC 4 cut(s) 902, 1547, 2838, 2922
AspLEI GCGC 1 cut(s) 2414
AspS9I GGNCC 9 cut(s) 767, 1584, 1962, 2333, 2349, 2472, 2526, 2559, 2895
AsuC2I CCSGG 8 cut(s) 85, 131, 159, 206, 485, 532, 1061, 2432
AsuII TTCGAA 1 cut(s) 1243
AvaI CYCGRG 3 cut(s) 377, 1450, 1931
AvaII GGWCC 5 cut(s) 767, 1584, 1962, 2472, 2559
BalI TGGCCA 1 cut(s) 2501
BamHI GGATCC 2 cut(s) 1632, 2211
BanI GGYRCC 1 cut(s) 2777
BanII GRGCYC 2 cut(s) 384, 1263
BbsI GAAGAC 2 cut(s) 2120, 2724
Bbv12I GWGCWC 2 cut(s) 384, 1263
BbvCI CCTCAGC 1 cut(s) 1727
BbvI GCAGC 8 cut(s) 83, 174, 1116, 1353, 2628, 2710, 2766, 2863
BccI CCATC 2 cut(s) 719, 2947
BceAI ACGGC 6 cut(s) 343, 1282, 1336, 1354, 1480, 2581
BcgI CGANNNNNNTGC 2 cut(s) 1560, 1594
BciVI GTATCC 2 cut(s) 82, 1229
BcnI CCSGG 8 cut(s) 85, 131, 159, 206, 485, 532, 1061, 2432
BcoDI GTCTC 4 cut(s) 730, 1108, 1307, 1753
BfaI CTAG 1 cut(s) 1814
BfmI CTRYAG 2 cut(s) 659, 2623
BfrI CTTAAG 1 cut(s) 1385
BfuAI ACCTGC 3 cut(s) 206, 1786, 1871
BfuI GTATCC 2 cut(s) 82, 1229
BglII AGATCT 4 cut(s) 886, 1029, 1389, 1877
Bme1390I CCNGG 8 cut(s) 85, 131, 159, 206, 485, 532, 1061, 2432
Bme18I GGWCC 5 cut(s) 767, 1584, 1962, 2472, 2559
BmeRI GACNNNNNGTC 1 cut(s) 2205
BmeT110I CYCGRG 3 cut(s) 377, 1450, 1931
BmgBI CACGTC 2 cut(s) 1462, 2437
BmgT120I GGNCC 9 cut(s) 767, 1584, 1962, 2333, 2349, 2472, 2526, 2559, 2895
BmrFI CCNGG 8 cut(s) 85, 131, 159, 206, 485, 532, 1061, 2432
BmrI ACTGGG 2 cut(s) 160, 1582
BmsI GCATC 3 cut(s) 83, 513, 2034
BmuI ACTGGG 2 cut(s) 160, 1582
BpiI GAAGAC 2 cut(s) 2120, 2724
BpmI CTGGAG 3 cut(s) 278, 1378, 2562
Bpu10I CCTNAGC 1 cut(s) 1727
Bpu14I TTCGAA 1 cut(s) 1243
BpuMI CCSGG 8 cut(s) 85, 131, 159, 206, 485, 532, 1061, 2432
Bsa29I ATCGAT 1 cut(s) 2799
BsaBI GATNNNNATC 1 cut(s) 1848
BsaHI GRCGYC 3 cut(s) 1101, 1269, 2619
BsaI GGTCTC 1 cut(s) 1307
BsaJI CCNNGG 5 cut(s) 84, 1930, 2127, 2383, 2804
Bse118I RCCGGY 5 cut(s) 473, 498, 545, 1657, 1697
Bse1I ACTGG 6 cut(s) 155, 1303, 1588, 2474, 2502, 2662
Bse3DI GCAATG 6 cut(s) 407, 686, 1182, 2364, 2733, 2764
Bse8I GATNNNNATC 1 cut(s) 1848
BseAI TCCGGA 4 cut(s) 876, 1121, 1193, 1840
BseCI ATCGAT 1 cut(s) 2799
BseDI CCNNGG 5 cut(s) 84, 1930, 2127, 2383, 2804
BseJI GATNNNNATC 1 cut(s) 1848
BseMI GCAATG 6 cut(s) 407, 686, 1182, 2364, 2733, 2764
BseMII CTCAG 6 cut(s) 107, 840, 1718, 2181, 2313, 2598
BseNI ACTGG 6 cut(s) 155, 1303, 1588, 2474, 2502, 2662
BseRI GAGGAG 5 cut(s) 811, 962, 1553, 2051, 2760
BseX3I CGGCCG 2 cut(s) 496, 1072
BseXI GCAGC 8 cut(s) 83, 174, 1116, 1353, 2628, 2710, 2766, 2863
BseYI CCCAGC 1 cut(s) 2555
BsgI GTGCAG 3 cut(s) 30, 1590, 1855
Bsh1236I CGCG 1 cut(s) 2075
Bsh1285I CGRYCG 5 cut(s) 205, 499, 1075, 2373, 2948
BshNI GGYRCC 1 cut(s) 2777
BshTI ACCGGT 2 cut(s) 545, 1697
BshVI ATCGAT 1 cut(s) 2799
BsiEI CGRYCG 5 cut(s) 205, 499, 1075, 2373, 2948
BsiHKAI GWGCWC 2 cut(s) 384, 1263
BsiHKCI CYCGRG 3 cut(s) 377, 1450, 1931
BslFI GGGAC 4 cut(s) 221, 1782, 2212, 2447
BsmAI GTCTC 4 cut(s) 730, 1108, 1307, 1753
BsmBI CGTCTC 3 cut(s) 730, 1108, 1753
BsmFI GGGAC 4 cut(s) 221, 1782, 2212, 2447
BsmI GAATGC 2 cut(s) 282, 2879
Bso31I GGTCTC 1 cut(s) 1307
BsoBI CYCGRG 3 cut(s) 377, 1450, 1931
Bsp119I TTCGAA 1 cut(s) 1243
Bsp1286I GDGCHC 2 cut(s) 384, 1263
Bsp13I TCCGGA 4 cut(s) 876, 1121, 1193, 1840
Bsp19I CCATGG 1 cut(s) 2383
Bsp68I TCGCGA 1 cut(s) 2075
BspCNI CTCAG 6 cut(s) 106, 841, 1719, 2182, 2314, 2599
BspDI ATCGAT 1 cut(s) 2799
BspEI TCCGGA 4 cut(s) 876, 1121, 1193, 1840
BspFNI CGCG 1 cut(s) 2075
BspMI ACCTGC 3 cut(s) 206, 1786, 1871
BspPI GGATC 9 cut(s) 1627, 1640, 1683, 1851, 1857, 2206, 2219, 2795, 2807
BspQI GCTCTTC 2 cut(s) 2606, 2868
BspT104I TTCGAA 1 cut(s) 1243
BspT107I GGYRCC 1 cut(s) 2777
BspTI CTTAAG 1 cut(s) 1385
BspTNI GGTCTC 1 cut(s) 1307
BsrDI GCAATG 6 cut(s) 407, 686, 1182, 2364, 2733, 2764
BsrFI RCCGGY 5 cut(s) 473, 498, 545, 1657, 1697
BsrI ACTGG 6 cut(s) 155, 1303, 1588, 2474, 2502, 2662
BssAI RCCGGY 5 cut(s) 473, 498, 545, 1657, 1697
BssECI CCNNGG 5 cut(s) 84, 1930, 2127, 2383, 2804
BssNI GRCGYC 3 cut(s) 1101, 1269, 2619
BssT1I CCWWGG 2 cut(s) 2383, 2804
Bst4CI ACNGT 9 cut(s) 51, 67, 123, 1764, 2128, 2264, 2312, 2358, 2949
Bst6I CTCTTC 6 cut(s) 674, 2028, 2435, 2606, 2829, 2868
BstACI GRCGYC 3 cut(s) 1101, 1269, 2619
BstAFI CTTAAG 1 cut(s) 1385
BstBI TTCGAA 1 cut(s) 1243
BstC8I GCNNGC 5 cut(s) 225, 781, 983, 1415, 2319
BstDEI CTNAG 8 cut(s) 93, 397, 849, 883, 1727, 2190, 2322, 2607
BstDSI CCRYGG 2 cut(s) 2127, 2383
BstEII GGTNACC 2 cut(s) 1820, 2002
BstENI CCTNNNNNAGG 2 cut(s) 2189, 2742
BstFNI CGCG 1 cut(s) 2075
BstHHI GCGC 1 cut(s) 2414
BstMAI GTCTC 4 cut(s) 730, 1108, 1307, 1753
BstMCI CGRYCG 5 cut(s) 205, 499, 1075, 2373, 2948
BstMWI GCNNNNNNNGC 3 cut(s) 80, 814, 2348
BstNSI RCATGY 3 cut(s) 1549, 2321, 2826
BstPI GGTNACC 2 cut(s) 1820, 2002
BstSCI CCNGG 8 cut(s) 83, 129, 157, 204, 483, 530, 1059, 2430
BstSFI CTRYAG 2 cut(s) 659, 2623
BstUI CGCG 1 cut(s) 2075
BstV1I GCAGC 8 cut(s) 83, 174, 1116, 1353, 2628, 2710, 2766, 2863
BstV2I GAAGAC 2 cut(s) 2120, 2724
BstX2I RGATCY 8 cut(s) 886, 1029, 1389, 1632, 1675, 1843, 1877, 2211
BstXI CCANNNNNNTGG 2 cut(s) 183, 565
BstYI RGATCY 8 cut(s) 886, 1029, 1389, 1632, 1675, 1843, 1877, 2211
BstZI CGGCCG 2 cut(s) 496, 1072
Bsu15I ATCGAT 1 cut(s) 2799
BsuI GTATCC 2 cut(s) 82, 1229
BsuTUI ATCGAT 1 cut(s) 2799
BtgI CCRYGG 2 cut(s) 2127, 2383
BtgZI GCGATG 2 cut(s) 1156, 2555
BtrI CACGTC 2 cut(s) 1462, 2437
BtsI GCAGTG 2 cut(s) 1578, 2871
BtsIMutI CAGTG 3 cut(s) 47, 1578, 2871
BtuMI TCGCGA 1 cut(s) 2075
BveI ACCTGC 3 cut(s) 206, 1786, 1871
Cac8I GCNNGC 5 cut(s) 225, 781, 983, 1415, 2319
CaiI CAGNNNCTG 1 cut(s) 1528
CfoI GCGC 1 cut(s) 2414
Cfr10I RCCGGY 5 cut(s) 473, 498, 545, 1657, 1697
Cfr13I GGNCC 9 cut(s) 767, 1584, 1962, 2333, 2349, 2472, 2526, 2559, 2895
ClaI ATCGAT 1 cut(s) 2799
CpoI CGGWCCG 1 cut(s) 767
CseI GACGC 2 cut(s) 814, 1258
Csp6I GTAC 5 cut(s) 549, 820, 1765, 1922, 2359
CspAI ACCGGT 2 cut(s) 545, 1697
CspI CGGWCCG 1 cut(s) 767
CviQI GTAC 5 cut(s) 549, 820, 1765, 1922, 2359
DdeI CTNAG 8 cut(s) 93, 397, 849, 883, 1727, 2190, 2322, 2607
DraIII CACNNNGTG 1 cut(s) 220
DrdI GACNNNNNNGTC 1 cut(s) 2369
DriI GACNNNNNGTC 1 cut(s) 2205
DseDI GACNNNNNNGTC 1 cut(s) 2369
EaeI YGGCCR 5 cut(s) 81, 166, 496, 1072, 2499
EagI CGGCCG 2 cut(s) 496, 1072
Eam1104I CTCTTC 6 cut(s) 674, 2028, 2435, 2606, 2829, 2868
Eam1105I GACNNNNNGTC 1 cut(s) 2205
EarI CTCTTC 6 cut(s) 674, 2028, 2435, 2606, 2829, 2868
EciI GGCGGA 2 cut(s) 301, 2357
Ecl136II GAGCTC 2 cut(s) 382, 1261
EclXI CGGCCG 2 cut(s) 496, 1072
Eco130I CCWWGG 2 cut(s) 2383, 2804
Eco24I GRGCYC 2 cut(s) 384, 1263
Eco31I GGTCTC 1 cut(s) 1307
Eco32I GATATC 2 cut(s) 481, 1359
Eco47I GGWCC 5 cut(s) 767, 1584, 1962, 2472, 2559
Eco52I CGGCCG 2 cut(s) 496, 1072
Eco53kI GAGCTC 2 cut(s) 382, 1261
Eco57I CTGAAG 3 cut(s) 1532, 2132, 2760
Eco88I CYCGRG 3 cut(s) 377, 1450, 1931
Eco91I GGTNACC 2 cut(s) 1820, 2002
EcoICRI GAGCTC 2 cut(s) 382, 1261
EcoNI CCTNNNNNAGG 2 cut(s) 2189, 2742
EcoO109I RGGNCCY 2 cut(s) 2559, 2895
EcoO65I GGTNACC 2 cut(s) 1820, 2002
EcoRI GAATTC 1 cut(s) 1250
EcoRV GATATC 2 cut(s) 481, 1359
EcoT14I CCWWGG 2 cut(s) 2383, 2804
EcoT22I ATGCAT 2 cut(s) 59, 2590
EcoT38I GRGCYC 2 cut(s) 384, 1263
ErhI CCWWGG 2 cut(s) 2383, 2804
Esp3I CGTCTC 3 cut(s) 730, 1108, 1753
FaqI GGGAC 4 cut(s) 221, 1782, 2212, 2447
FauI CCCGC 4 cut(s) 251, 684, 2144, 2484
FblI GTMKAC 3 cut(s) 749, 1098, 2622
FriOI GRGCYC 2 cut(s) 384, 1263
FspBI CTAG 1 cut(s) 1814
GlaI GCGC 1 cut(s) 2413
GsaI CCCAGC 1 cut(s) 2559
GsuI CTGGAG 3 cut(s) 278, 1378, 2562
HgaI GACGC 2 cut(s) 814, 1258
HhaI GCGC 1 cut(s) 2414
Hin1I GRCGYC 3 cut(s) 1101, 1269, 2619
Hin6I GCGC 1 cut(s) 2412
HinP1I GCGC 1 cut(s) 2412
HincII GTYRAC 5 cut(s) 585, 1099, 1593, 1719, 2820
HindII GTYRAC 5 cut(s) 585, 1099, 1593, 1719, 2820
HindIII AAGCTT 2 cut(s) 1436, 1968
HpyCH4III ACNGT 9 cut(s) 51, 67, 123, 1764, 2128, 2264, 2312, 2358, 2949
HpyCH4IV ACGT 8 cut(s) 1101, 1115, 1211, 1239, 1461, 1746, 2436, 2619
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 814, 2348
HpyF3I CTNAG 8 cut(s) 93, 397, 849, 883, 1727, 2190, 2322, 2607
HpySE526I ACGT 8 cut(s) 1101, 1115, 1211, 1239, 1461, 1746, 2436, 2619
Hsp92I GRCGYC 3 cut(s) 1101, 1269, 2619
HspAI GCGC 1 cut(s) 2412
Kpn2I TCCGGA 4 cut(s) 876, 1121, 1193, 1840
LguI GCTCTTC 2 cut(s) 2606, 2868
LmnI GCTCC 8 cut(s) 365, 387, 798, 1507, 1597, 1871, 2419, 2581
Lsp1109I GCAGC 8 cut(s) 83, 174, 1116, 1353, 2628, 2710, 2766, 2863
LweI GCATC 3 cut(s) 83, 513, 2034
MaeI CTAG 1 cut(s) 1814
MaeII ACGT 8 cut(s) 1101, 1115, 1211, 1239, 1461, 1746, 2436, 2619
MfeI CAATTG 3 cut(s) 181, 927, 941
MflI RGATCY 8 cut(s) 886, 1029, 1389, 1632, 1675, 1843, 1877, 2211
MhlI GDGCHC 2 cut(s) 384, 1263
MlsI TGGCCA 1 cut(s) 2501
MluNI TGGCCA 1 cut(s) 2501
MlyI GAGTC 6 cut(s) 283, 580, 701, 956, 2669, 2674
MmeI TCCRAC 8 cut(s) 150, 382, 1610, 1692, 1907, 2038, 2189, 2239
Mox20I TGGCCA 1 cut(s) 2501
Mph1103I ATGCAT 2 cut(s) 59, 2590
MroI TCCGGA 4 cut(s) 876, 1121, 1193, 1840
MroXI GAANNNNTTC 4 cut(s) 902, 1547, 2838, 2922
MscI TGGCCA 1 cut(s) 2501
MslI CAYNNNNRTG 3 cut(s) 52, 369, 1175
Msp20I TGGCCA 1 cut(s) 2501
MspA1I CMGCKG 1 cut(s) 165
MspCI CTTAAG 1 cut(s) 1385
MspR9I CCNGG 8 cut(s) 85, 131, 159, 206, 485, 532, 1061, 2432
MunI CAATTG 3 cut(s) 181, 927, 941
Mva1269I GAATGC 2 cut(s) 282, 2879
MvnI CGCG 1 cut(s) 2075
MwoI GCNNNNNNNGC 3 cut(s) 80, 814, 2348
NciI CCSGG 8 cut(s) 85, 131, 159, 206, 485, 532, 1061, 2432
NcoI CCATGG 1 cut(s) 2383
NmeAIII GCCGAG 2 cut(s) 600, 1050
NmuCI GTSAC 4 cut(s) 802, 1312, 2306, 2544
NruI TCGCGA 1 cut(s) 2075
NsiI ATGCAT 2 cut(s) 59, 2590
NspI RCATGY 3 cut(s) 1549, 2321, 2826
NspV TTCGAA 1 cut(s) 1243
PaeI GCATGC 1 cut(s) 2321
PaeR7I CTCGAG 2 cut(s) 377, 1450
PaqCI CACCTGC 1 cut(s) 206
PciI ACATGT 1 cut(s) 1545
PciSI GCTCTTC 2 cut(s) 2606, 2868
PcsI WCGNNNNNNNCGW 9 cut(s) 141, 384, 720, 819, 1112, 1245, 1269, 1448, 1943
PctI GAATGC 2 cut(s) 282, 2879
PdmI GAANNNNTTC 4 cut(s) 902, 1547, 2838, 2922
PflFI GACNNNGTC 1 cut(s) 1268
PfoI TCCNGGA 2 cut(s) 530, 1059
PinAI ACCGGT 2 cut(s) 545, 1697
PleI GAGTC 6 cut(s) 283, 580, 701, 955, 2669, 2673
PpsI GAGTC 6 cut(s) 283, 580, 701, 955, 2669, 2673
PpuMI RGGWCCY 1 cut(s) 2559
PscI ACATGT 1 cut(s) 1545
PsiI TTATAA 1 cut(s) 2100
Psp124BI GAGCTC 2 cut(s) 384, 1263
Psp5II RGGWCCY 1 cut(s) 2559
PspEI GGTNACC 2 cut(s) 1820, 2002
PspFI CCCAGC 1 cut(s) 2555
PspPI GGNCC 9 cut(s) 767, 1584, 1962, 2333, 2349, 2472, 2526, 2559, 2895
PspPPI RGGWCCY 1 cut(s) 2559
PstNI CAGNNNCTG 1 cut(s) 1528
PsuI RGATCY 8 cut(s) 886, 1029, 1389, 1632, 1675, 1843, 1877, 2211
PsyI GACNNNGTC 1 cut(s) 1268
RruI TCGCGA 1 cut(s) 2075
RsaI GTAC 5 cut(s) 550, 821, 1766, 1923, 2360
RsaNI GTAC 5 cut(s) 549, 820, 1765, 1922, 2359
RseI CAYNNNNRTG 3 cut(s) 52, 369, 1175
Rsr2I CGGWCCG 1 cut(s) 767
RsrII CGGWCCG 1 cut(s) 767
SacI GAGCTC 2 cut(s) 384, 1263
SalI GTCGAC 1 cut(s) 1097
SapI GCTCTTC 2 cut(s) 2606, 2868
Sau96I GGNCC 9 cut(s) 767, 1584, 1962, 2333, 2349, 2472, 2526, 2559, 2895
SchI GAGTC 6 cut(s) 283, 580, 701, 956, 2669, 2674
ScrFI CCNGG 8 cut(s) 85, 131, 159, 206, 485, 532, 1061, 2432
SduI GDGCHC 2 cut(s) 384, 1263
SfaNI GCATC 3 cut(s) 83, 513, 2034
SfcI CTRYAG 2 cut(s) 659, 2623
Sfr274I CTCGAG 2 cut(s) 377, 1450
SfuI TTCGAA 1 cut(s) 1243
SgrAI CRCCGGYG 2 cut(s) 473, 1697
SinI GGWCC 5 cut(s) 767, 1584, 1962, 2472, 2559
SlaI CTCGAG 2 cut(s) 377, 1450
SmiMI CAYNNNNRTG 3 cut(s) 52, 369, 1175
SmlI CTYRAG 3 cut(s) 377, 1385, 1450
SmoI CTYRAG 3 cut(s) 377, 1385, 1450
SphI GCATGC 1 cut(s) 2321
SspI AATATT 1 cut(s) 2227
SspMI CTAG 1 cut(s) 1814
SstI GAGCTC 2 cut(s) 384, 1263
StyD4I CCNGG 8 cut(s) 83, 129, 157, 204, 483, 530, 1059, 2430
StyI CCWWGG 2 cut(s) 2383, 2804
TaaI ACNGT 9 cut(s) 51, 67, 123, 1764, 2128, 2264, 2312, 2358, 2949
TaiI ACGT 8 cut(s) 1104, 1118, 1214, 1242, 1464, 1749, 2439, 2622
TatI WGTACW 1 cut(s) 1921
TauI GCSGC 7 cut(s) 168, 699, 1021, 1367, 1495, 1993, 2778
TscAI CASTG 3 cut(s) 54, 1578, 2878
TseFI GTSAC 4 cut(s) 802, 1312, 2306, 2544
TseI GCWGC 8 cut(s) 71, 162, 1129, 1341, 2641, 2723, 2754, 2876
Tsp45I GTSAC 4 cut(s) 802, 1312, 2306, 2544
TspDTI ATGAA 6 cut(s) 749, 1159, 1334, 1392, 2046, 2295
TspRI CASTG 3 cut(s) 54, 1578, 2878
Tth111I GACNNNGTC 1 cut(s) 1268
Vha464I CTTAAG 1 cut(s) 1385
VpaK11BI GGWCC 5 cut(s) 767, 1584, 1962, 2472, 2559
XagI CCTNNNNNAGG 2 cut(s) 2189, 2742
XapI RAATTY 9 cut(s) 754, 1165, 1198, 1250, 1373, 1475, 1560, 1828, 1955
XceI RCATGY 3 cut(s) 1549, 2321, 2826
XcmI CCANNNNNNNNNTGG 1 cut(s) 2908
XhoI CTCGAG 2 cut(s) 377, 1450
XmiI GTMKAC 3 cut(s) 749, 1098, 2622
XmnI GAANNNNTTC 4 cut(s) 902, 1547, 2838, 2922
XspI CTAG 1 cut(s) 1814
ZraI GACGTC 2 cut(s) 1102, 2620
Zsp2I ATGCAT 2 cut(s) 59, 2590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.