Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0016385

Physical Location & Seq

Genomic Coordinates Forward (+)

27414501 .. 27415766

Gene Structure 1266 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1229800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGGAGGATCTTGAGATATCAGAGAAAAACACACCTGCAGATCACGACTTTCCTCCATACCATTTCAGATGTCCCATTTCTATGGAGCTCATGAAAGACCCCGTCACCATCTCCACGGGCGTCACCTATGAACGCAACAACATAGAGAAATGGTTCTTCTCCTACAAGAAGAAAACATGCCCAGCTACAATGCAAAGCCTTGAGAATTTTGACATGATCCCGAACCACACCCTCAAGAGGCTCATTGTTGCATGGCAAACCGAAGAGGATGCGAAAAATGTTATTTGCTCAACATCATCTACACCAAGGCCTTCAGCTAAGCATGACGAAATTCAAGCGCTCCTCAACACAATCATGTCATCCCCATTCAAGGTAAATTCGATAAAGAAACTTCGTTCCATCATCGACCTAGATGATGAGATGAAAAATGATTTTATTCGATCTAACGGGGTCGAAGTTCTTGTCCAAATTCTTGATCAAATTCTCTTGGAGAGCTCGGATTTCACAACGTTTAGGGCTTGTGAGGAGGCTCTTTGTATTCTTCACCAACTTCCAATATCGGGAGAAGGGAAAACATTTAAATTGTTATCGAAGCAAGAATCAACCAGATCGATGACTGTCATGCTTCAGCGAGGAAGCACTGAGGCACGGCTCCATACCATTACCATATTCAAGAAGATGGCGAAAACCGAATATGATTGGAGCTTCGTGATACAAGATCAAGGTATAGATTTTTTTAAATCCTTGTTGGAGCTCCTTTCGGATGAAATTTGTAGCAAAGCAAGTTCATGTGCCTTGAAAGTCTTGATAGAAATATTAACAGCATCAAAGAAAAACAAGTTGAGGGCAATTGAAGCAGGCGCAGTTTGTGTCCTTATAGAGCTACTGCCAGACTCCAACAGATCCAAATGTGAAAAAATGTTGCAAGTGATAAAGTTGTTATGTGAATGTTCTGAGGGAAGGCAGGCCTTGGTGGAACATAGTATGGGCATTGTGGCAGTTTCCAAAAACATGTTGCATGTTTCTAATGCTGCCACCAAGATTGGGGTGAAGATCATATGGTTGATTTGTAATTTTCATCCGACGGAGAGGGTGTTGGAGGAAATGTTGATTTGTGGATCGGTGAAGAAGCTTCTGGCATTGTTGCAGATGGATAGCAGATCTTCTACAAAGGATAAGGTGGTGAAGATCTTCAAGATGCATGGAAATTCATGGAAACGATATCCATGTTTCCCTTGGGATTTGAAGGATTATTTGGATTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010033 GO:0010035 GO:0010200 GO:0010243 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051865 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

U-box

Protein Analysis

422

Amino Acids

47.96

Weight (kDa)

7.5

Isoelectric Point (pI)

47.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
U-box	PF04564	19 - 88	2.6e-17	U-box domain
ARM_PUB	PF25598	111 - 411	1.5e-54	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016385)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g08000
malus_domestica	MD02G1229800.v1.1 MD07G1083300.v1.1
prunus_persica	Prupe.2G109000_v2.0.a1
pyrus_communis	pycom07g06670
rosa_chinensis	RchiOBHm_Chr1g0341311
rosa_laevigata	RLG00000029083
rosa_multiflora	Rmu_sc0004092.1_g000049
rosa_roxburghii	Rroxscaffold_4G00311950
rosa_rugosa	Rorug01G0157700.1
rosa_samantha	Rh1AG172300 Rh1BG140300 Rh1CG160400 Rh1DG172600
rosa_wichuraiana	Rw1G014370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	43
Acc36I	ACCTGC	1 cut(s)	43
AclI	AACGTT	1 cut(s)	509
AclWI	GGATC	4 cut(s)	15, 211, 897, 1126
AcsI	RAATTY	7 cut(s)	205, 330, 376, 468, 480, 768, 1207
AcuI	CTGAAG	2 cut(s)	297, 611
AcyI	GRCGYC	1 cut(s)	120
AfeI	AGCGCT	1 cut(s)	339
AfiI	CCNNNNNNNGG	4 cut(s)	237, 370, 560, 1044
AflIII	ACRYGT	1 cut(s)	1011
AgsI	TTSAA	7 cut(s)	335, 370, 673, 799, 854, 1195, 1246
AjuI	GAANNNNNNNTTGG	1 cut(s)	1238
AluBI	AGCT	8 cut(s)	88, 185, 317, 495, 705, 754, 883, 1132
AluI	AGCT	8 cut(s)	88, 185, 317, 495, 705, 754, 883, 1132
Alw21I	GWGCWC	3 cut(s)	90, 497, 756
AlwI	GGATC	4 cut(s)	15, 211, 897, 1126
Aor51HI	AGCGCT	1 cut(s)	339
AoxI	GGCC	2 cut(s)	308, 966
ApeKI	GCWGC	1 cut(s)	1031
ApoI	RAATTY	7 cut(s)	205, 330, 376, 468, 480, 768, 1207
ArsI	GACNNNNNNTTYG	2 cut(s)	447, 479
Asp700I	GAANNNNTTC	2 cut(s)	152, 1190
AspLEI	GCGC	2 cut(s)	340, 863
AsuHPI	GGTGA	6 cut(s)	97, 115, 536, 1060, 1135, 1195
BanII	GRGCYC	3 cut(s)	90, 497, 756
Bbv12I	GWGCWC	3 cut(s)	90, 497, 756
BbvI	GCAGC	1 cut(s)	1018
BccI	CCATC	4 cut(s)	116, 407, 673, 1144
BceAI	ACGGC	1 cut(s)	665
BcgI	CGANNNNNNTGC	2 cut(s)	251, 285
BclI	TGATCA	1 cut(s)	475
BfaI	CTAG	1 cut(s)	410
BfmI	CTRYAG	1 cut(s)	36
BfoI	RGCGCY	1 cut(s)	341
BfuAI	ACCTGC	1 cut(s)	43
BglII	AGATCT	2 cut(s)	1160, 1188
BisI	GCNGC	1 cut(s)	1032
BlpI	GCTNAGC	1 cut(s)	318
BlsI	GCNGC	1 cut(s)	1033
BmiI	GGNNCC	1 cut(s)	653
BmsI	GCATC	3 cut(s)	259, 833, 1188
Bpu1102I	GCTNAGC	1 cut(s)	318
BpuEI	CTTGAG	3 cut(s)	32, 218, 221
Bsa29I	ATCGAT	1 cut(s)	611
BsaHI	GRCGYC	1 cut(s)	120
BsaJI	CCNNGG	4 cut(s)	114, 305, 969, 1235
BsaXI	ACNNNNNCTCC	4 cut(s)	1079, 1091, 1109, 1121
Bsc4I	CCNNNNNNNGG	4 cut(s)	237, 370, 560, 1044
BseCI	ATCGAT	1 cut(s)	611
BseDI	CCNNGG	4 cut(s)	114, 305, 969, 1235
BseGI	GGATG	4 cut(s)	274, 359, 769, 1078
BseLI	CCNNNNNNNGG	4 cut(s)	237, 370, 560, 1044
BseMII	CTCAG	2 cut(s)	633, 945
BseRI	GAGGAG	2 cut(s)	332, 539
BseXI	GCAGC	1 cut(s)	1018
BseYI	CCCAGC	1 cut(s)	181
BshFI	GGCC	2 cut(s)	310, 968
BshVI	ATCGAT	1 cut(s)	611
BsiHKAI	GWGCWC	3 cut(s)	90, 497, 756
BslFI	GGGAC	1 cut(s)	57
BslI	CCNNNNNNNGG	4 cut(s)	237, 370, 560, 1044
BsmFI	GGGAC	1 cut(s)	57
BsnI	GGCC	2 cut(s)	310, 968
Bsp1286I	GDGCHC	3 cut(s)	90, 497, 756
Bsp1720I	GCTNAGC	1 cut(s)	318
BspANI	GGCC	2 cut(s)	310, 968
BspCNI	CTCAG	2 cut(s)	634, 946
BspDI	ATCGAT	1 cut(s)	611
BspHI	TCATGA	1 cut(s)	90
BspLI	GGNNCC	1 cut(s)	653
BspMAI	CTGCAG	1 cut(s)	40
BspMI	ACCTGC	1 cut(s)	43
BspPI	GGATC	4 cut(s)	15, 211, 897, 1126
BssECI	CCNNGG	4 cut(s)	114, 305, 969, 1235
BssNI	GRCGYC	1 cut(s)	120
BssT1I	CCWWGG	3 cut(s)	305, 969, 1235
Bst4CI	ACNGT	1 cut(s)	619
Bst6I	CTCTTC	1 cut(s)	258
BstACI	GRCGYC	1 cut(s)	120
BstC8I	GCNNGC	2 cut(s)	859, 966
BstDEI	CTNAG	3 cut(s)	318, 642, 954
BstDSI	CCRYGG	1 cut(s)	114
BstF5I	GGATG	4 cut(s)	274, 359, 769, 1078
BstH2I	RGCGCY	1 cut(s)	341
BstHHI	GCGC	2 cut(s)	340, 863
BstMWI	GCNNNNNNNGC	1 cut(s)	854
BstNSI	RCATGY	3 cut(s)	180, 1015, 1022
BstSFI	CTRYAG	1 cut(s)	36
BstV1I	GCAGC	1 cut(s)	1018
BstX2I	RGATCY	4 cut(s)	7, 902, 1160, 1188
BstXI	CCANNNNNNTGG	1 cut(s)	82
BstYI	RGATCY	4 cut(s)	7, 902, 1160, 1188
Bsu15I	ATCGAT	1 cut(s)	611
BsuRI	GGCC	2 cut(s)	310, 968
BsuTUI	ATCGAT	1 cut(s)	611
BtgI	CCRYGG	1 cut(s)	114
BtsCI	GGATG	4 cut(s)	274, 359, 769, 1078
BtsIMutI	CAGTG	1 cut(s)	639
BveI	ACCTGC	1 cut(s)	43
Cac8I	GCNNGC	2 cut(s)	859, 966
CciI	TCATGA	1 cut(s)	90
CfoI	GCGC	2 cut(s)	340, 863
ClaI	ATCGAT	1 cut(s)	611
CseI	GACGC	1 cut(s)	109
CspCI	CAANNNNNGTGG	2 cut(s)	1024, 1059
DdeI	CTNAG	3 cut(s)	318, 642, 954
DraI	TTTAAA	2 cut(s)	580, 739
Eam1104I	CTCTTC	1 cut(s)	258
EarI	CTCTTC	1 cut(s)	258
Ecl136II	GAGCTC	3 cut(s)	88, 495, 754
Eco130I	CCWWGG	3 cut(s)	305, 969, 1235
Eco147I	AGGCCT	2 cut(s)	310, 968
Eco24I	GRGCYC	3 cut(s)	90, 497, 756
Eco32I	GATATC	2 cut(s)	18, 1223
Eco47III	AGCGCT	1 cut(s)	339
Eco53kI	GAGCTC	3 cut(s)	88, 495, 754
Eco57I	CTGAAG	2 cut(s)	297, 611
EcoICRI	GAGCTC	3 cut(s)	88, 495, 754
EcoRV	GATATC	2 cut(s)	18, 1223
EcoT14I	CCWWGG	3 cut(s)	305, 969, 1235
EcoT22I	ATGCAT	1 cut(s)	1203
EcoT38I	GRGCYC	3 cut(s)	90, 497, 756
ErhI	CCWWGG	3 cut(s)	305, 969, 1235
FaqI	GGGAC	1 cut(s)	57
FauNDI	CATATG	1 cut(s)	1058
FbaI	TGATCA	1 cut(s)	475
Fnu4HI	GCNGC	1 cut(s)	1032
FokI	GGATG	4 cut(s)	281, 346, 776, 1065
FriOI	GRGCYC	3 cut(s)	90, 497, 756
Fsp4HI	GCNGC	1 cut(s)	1032
FspBI	CTAG	1 cut(s)	410
GlaI	GCGC	2 cut(s)	339, 862
GluI	GCNGC	1 cut(s)	1032
GsaI	CCCAGC	1 cut(s)	185
HaeII	RGCGCY	1 cut(s)	341
HaeIII	GGCC	2 cut(s)	310, 968
HgaI	GACGC	1 cut(s)	109
HhaI	GCGC	2 cut(s)	340, 863
Hin1I	GRCGYC	1 cut(s)	120
Hin6I	GCGC	2 cut(s)	338, 861
HinP1I	GCGC	2 cut(s)	338, 861
HindIII	AAGCTT	1 cut(s)	1130
HinfI	GANTC	2 cut(s)	599, 893
HphI	GGTGA	6 cut(s)	97, 115, 536, 1060, 1135, 1195
Hpy188I	TCNGA	6 cut(s)	22, 68, 499, 763, 955, 1083
Hpy99I	CGWCG	1 cut(s)	1087
HpyAV	CCTTC	4 cut(s)	321, 560, 954, 1240
HpyCH4III	ACNGT	1 cut(s)	619
HpyCH4IV	ACGT	1 cut(s)	509
HpyCH4V	TGCA	7 cut(s)	38, 193, 251, 925, 1018, 1147, 1201
HpyF10VI	GCNNNNNNNGC	1 cut(s)	854
HpyF3I	CTNAG	3 cut(s)	318, 642, 954
HpySE526I	ACGT	1 cut(s)	509
Hsp92I	GRCGYC	1 cut(s)	120
HspAI	GCGC	2 cut(s)	338, 861
Ksp22I	TGATCA	1 cut(s)	475
LmnI	GCTCC	6 cut(s)	85, 345, 657, 702, 751, 759
LpnPI	CCDG	7 cut(s)	48, 195, 619, 843, 903, 950, 1121
Lsp1109I	GCAGC	1 cut(s)	1018
LweI	GCATC	3 cut(s)	259, 833, 1188
MaeI	CTAG	1 cut(s)	410
MaeII	ACGT	1 cut(s)	509
MaeIII	GTNAC	2 cut(s)	103, 121
MfeI	CAATTG	1 cut(s)	849
MflI	RGATCY	4 cut(s)	7, 902, 1160, 1188
MhlI	GDGCHC	3 cut(s)	90, 497, 756
MlyI	GAGTC	1 cut(s)	887
MmeI	TCCRAC	4 cut(s)	729, 921, 1077, 1106
Mph1103I	ATGCAT	1 cut(s)	1203
MroXI	GAANNNNTTC	2 cut(s)	152, 1190
MseI	TTAA	3 cut(s)	579, 738, 818
MslI	CAYNNNNRTG	2 cut(s)	80, 353
MunI	CAATTG	1 cut(s)	849
MwoI	GCNNNNNNNGC	1 cut(s)	854
NdeI	CATATG	1 cut(s)	1058
NlaIV	GGNNCC	1 cut(s)	653
NmuCI	GTSAC	2 cut(s)	103, 121
NsiI	ATGCAT	1 cut(s)	1203
NspI	RCATGY	3 cut(s)	180, 1015, 1022
PagI	TCATGA	1 cut(s)	90
PaqCI	CACCTGC	1 cut(s)	43
PceI	AGGCCT	2 cut(s)	310, 968
PciI	ACATGT	1 cut(s)	1011
PdmI	GAANNNNTTC	2 cut(s)	152, 1190
PfeI	GAWTC	1 cut(s)	599
PflFI	GACNNNGTC	1 cut(s)	101
PkrI	GCNGC	1 cut(s)	1033
PleI	GAGTC	1 cut(s)	887
PpsI	GAGTC	1 cut(s)	887
PscI	ACATGT	1 cut(s)	1011
Psp124BI	GAGCTC	3 cut(s)	90, 497, 756
Psp1406I	AACGTT	1 cut(s)	509
PspFI	CCCAGC	1 cut(s)	181
PspN4I	GGNNCC	1 cut(s)	653
PstI	CTGCAG	1 cut(s)	40
PsuI	RGATCY	4 cut(s)	7, 902, 1160, 1188
PsyI	GACNNNGTC	1 cut(s)	101
RseI	CAYNNNNRTG	2 cut(s)	80, 353
SacI	GAGCTC	3 cut(s)	90, 497, 756
SaqAI	TTAA	3 cut(s)	579, 738, 818
SatI	GCNGC	1 cut(s)	1032
SchI	GAGTC	1 cut(s)	887
SduI	GDGCHC	3 cut(s)	90, 497, 756
SfaNI	GCATC	3 cut(s)	259, 833, 1188
SfcI	CTRYAG	1 cut(s)	36
SmiI	ATTTAAAT	1 cut(s)	580
SmiMI	CAYNNNNRTG	2 cut(s)	80, 353
SmlI	CTYRAG	3 cut(s)	11, 200, 233
SmoI	CTYRAG	3 cut(s)	11, 200, 233
SseBI	AGGCCT	2 cut(s)	310, 968
SspI	AATATT	1 cut(s)	816
SspMI	CTAG	1 cut(s)	410
SstI	GAGCTC	3 cut(s)	90, 497, 756
StuI	AGGCCT	2 cut(s)	310, 968
StyI	CCWWGG	3 cut(s)	305, 969, 1235
SwaI	ATTTAAAT	1 cut(s)	580
TaaI	ACNGT	1 cut(s)	619
TaiI	ACGT	1 cut(s)	512
TaqI	TCGA	6 cut(s)	380, 405, 439, 453, 590, 611
TfiI	GAWTC	1 cut(s)	599
Tru1I	TTAA	3 cut(s)	579, 738, 818
Tru9I	TTAA	3 cut(s)	579, 738, 818
TscAI	CASTG	1 cut(s)	646
TseFI	GTSAC	2 cut(s)	103, 121
TseI	GCWGC	1 cut(s)	1031
Tsp45I	GTSAC	2 cut(s)	103, 121
TspDTI	ATGAA	7 cut(s)	107, 144, 437, 777, 780, 1067, 1200
TspGWI	ACGGA	1 cut(s)	1100
TspRI	CASTG	1 cut(s)	646
Tth111I	GACNNNGTC	1 cut(s)	101
XapI	RAATTY	7 cut(s)	205, 330, 376, 468, 480, 768, 1207
XceI	RCATGY	3 cut(s)	180, 1015, 1022
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1233
XmnI	GAANNNNTTC	2 cut(s)	152, 1190
XspI	CTAG	1 cut(s)	410
Zsp2I	ATGCAT	1 cut(s)	1203

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.