Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0019132

Physical Location & Seq

Genomic Coordinates Forward (+)

31663736 .. 31667985

Gene Structure 4250 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1262300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1998 bp

ATGAACTGGGTTCCAAGCTTCTTCTCTCCGCCCAAAACAAGCCGTCTGATTTGCCGCGGTCTTCACGCAAGTAAGCATTTCTCACCCCCAAACTCCGACAACATTGTCTTCAAAGCAGTTTGTGTTAATCTTCGACAGAGGAGATGGAAACTCTTAGAGCAGATATCCCACTGTCTAACCAGTTCATTAGTCAGCCGTGTTGTTCGCGAGTTTCGGAACTCGCCGCAGTTGGCCTTAGAATTTTATAACTGGGTTCGCTGGAACAGGAACAGGAACTTTCCTCAGTTTTTGGAATCTTCTTGTACTGTAATTCATGTTTTGATCGATTCTAGGAGGTACGATGATGCCTTGTCTGTTATGGAGAGTCTAATGGGGGCAGACGGTTTGCCCGCATTGGTTGTTTTGGAAGGGTTGAGTAATAGTTGTGGTGAAGGTTGTGGTTGTGCAAGCGCTGCAGTATTTGATGCATTTGTGAGGGCTTGTACTCAGTTCGGGGACTCCGACGGTGCTTATGAGGTGATCAAGAAGCTGAGACTGGATGGTTATTGGGTTTCGATTCACGCTTGGAATAACTTTCTTAACCATGTGATTAAGTTGAACGATATTGATAGGTTTTGGAAGATGTATAAGGAAATGCTTTCATATGGGTATGTTGAAAATGTTAATACCTTCAATTTGGTTATTTATGCTCTTAGTAAGGAACGCAAGTTACTAGAAGCAATGTCGGCATATTATCGGATGTTGAAGAGTGGAGTTTGGCCTAGTGTTGTTACTTTTAACATGATTATAGATGGAGCATGCAGGATAGGTGATATGGAACTTGCTTTGAAGGTTTTGAGAAAGATGGGGCTAATGTCGGAGCACTGTGTTACGCCCAATTCAGTTACTTACAATTGTATCATCAATGGGTTTTGCAAAATTGGTGGTTTATCGGTTGCAGAAGAAATCTGTGCTGAAATGACCGAGGTAGGTGTTCCGGTCAATTTGAGGACTTATGCAACTCTAGTAGATGGATATGCAAGACGGGGGAGTTTGGAGGTGGCACTTCAATTGTGCGATGAAATGGTGGAAAGGGGTTTGACCCCTAATCCTGTTGTTTACAATTCAATTATCCATCGGCTTTGCATAGAAGGAGATACTGAGGAAGCTTTTTCCTTGTTGTCTGACATGATTGAGAGGAATATATGCCCGGATCAATTCACCTACTCAATCCTCATCAAGGGGCTCTCTAGAAATGGGCTTGCGACGGAAGCAATTAGATTTCATAGACACATCCTTGAGAAGAACCTCGTCAAGGATGTTTTCTCCCACAATATCCTGATCGATTATCTGTGCAAAAGCAAAAATTTGAGAGCGGCCAAGCAATTGATGGGCAGCATGTTTGTTCGTGGTTTACTTCCTGACACCATCACCTATGGCACTTTGATTGATTGCCACTTCAAAGAAGGGAACATAGGAAATGCAGTTCAGATTTATGAAAAACTGATAGTGGGGAAGGAAAAACCTAATTTGGTGATATACAATTCTGTTATCAACGGGTTATGCAAAGAGGCATCAGTGGATATTGCAAAACTTTTGATGGATTCGTTACAGAGGATGGATGTTCTTGACATTATAACCTATAACACAATGATAAACGGGTATTTCATTAGTGGGATGATTGATCAGGCGTTTGTTTTGCTTTGGGAAATGGAAAAGGCTGGAATTTCATTGAATATAGTCACCTACAATATATTGATCAACTTTTTGTGCAAGTTTGGGTGTATTCAACAAGCAAAAGAACTTATGAAGGTAATGATTTCAAGGGGTGTGGTTCCTGATTTTATAACATACACCACACTCATTACCAATTTGAGTAAGAATTGCAGCCCCGAAGAAGTCATTGCATTGCATGACTACATGGTAATTGAGGGAGTAATTCCTGATAGGCAAACGTACACAGATATTGTCTGTCCATATCTTCCAGAAGAAAATTCAAAGATTAGCACAGTGGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

666

Amino Acids

75.19

Weight (kDa)

6.52

Isoelectric Point (pI)

41.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	220 - 268	2.4e-09	PPR repeat family
PPR_long	PF17177	228 - 361	3e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	251 - 282	6.9e-09	PPR repeat
PPR_2	PF13041	254 - 282	3.8e-06	PPR repeat family
PPR_1	PF12854	289 - 320	6.9e-09	PPR repeat
PPR_2	PF13041	292 - 341	4.1e-12	PPR repeat family
PPR_3	PF13812	316 - 372	8.3e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	324 - 356	8.4e-07	PPR repeat
PPR_2	PF13041	329 - 363	4.5e-06	PPR repeat family
PPR_3	PF13812	353 - 407	5.3e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	358 - 390	1e-08	PPR repeat
PPR_2	PF13041	362 - 410	1.1e-16	PPR repeat family
PPR	PF01535	365 - 395	2.9e-06	PPR repeat
PPR_1	PF12854	393 - 422	1.9e-06	PPR repeat
PPR_2	PF13041	432 - 480	6.1e-11	PPR repeat family
PPR_1	PF12854	463 - 494	1.2e-06	PPR repeat
PPR_2	PF13041	467 - 516	5.9e-09	PPR repeat family
PPR_1	PF12854	501 - 528	7.2e-06	PPR repeat
PPR_3	PF13812	529 - 582	1.7e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	536 - 565	1.7e-07	PPR repeat
PPR_2	PF13041	537 - 585	3.2e-16	PPR repeat family
PPR	PF01535	539 - 569	5e-07	PPR repeat
PPR_1	PF12854	568 - 599	3.6e-07	PPR repeat
PPR_2	PF13041	572 - 620	1.9e-13	PPR repeat family
PPR	PF01535	574 - 604	4.2e-06	PPR repeat
PPR_2	PF13041	606 - 647	6.5e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11710 AT1G11710
malus_domestica	MD02G1262300.v1.1 MD07G1057900.v1.1 MD07G1059400.v1.1
prunus_persica	Prupe.2G064100_v2.0.a1
pyrus_communis	pycom02g22430 pycom07g04410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	246, 1616, 1826
AasI	GACNNNNNNGTC	1 cut(s)	104
AccBSI	CCGCTC	1 cut(s)	1355
AccII	CGCG	2 cut(s)	57, 207
AciI	CCGC	6 cut(s)	29, 55, 57, 224, 390, 1355
AclWI	GGATC	1 cut(s)	1200
AcoI	YGGCCR	1 cut(s)	1356
AcsI	RAATTY	4 cut(s)	239, 1345, 1704, 1972
AfaI	GTAC	4 cut(s)	304, 338, 484, 1937
AfeI	AGCGCT	1 cut(s)	451
AfiI	CCNNNNNNNGG	2 cut(s)	1219, 1294
AloI	GAACNNNNNNTCC	2 cut(s)	1449, 1481
AluBI	AGCT	3 cut(s)	18, 529, 1148
AluI	AGCT	3 cut(s)	18, 529, 1148
Alw21I	GWGCWC	1 cut(s)	864
Alw26I	GTCTC	1 cut(s)	526
AlwI	GGATC	1 cut(s)	1200
Aor51HI	AGCGCT	1 cut(s)	451
AoxI	GGCC	3 cut(s)	231, 758, 1356
ApeKI	GCWGC	3 cut(s)	452, 1374, 1866
ApoI	RAATTY	4 cut(s)	239, 1345, 1704, 1972
AspLEI	GCGC	1 cut(s)	452
AsuC2I	CCSGG	1 cut(s)	1190
AsuHPI	GGTGA	8 cut(s)	75, 440, 529, 821, 1192, 1402, 1525, 1714
BaeI	ACNNNNGTAYC	2 cut(s)	880, 913
BanII	GRGCYC	1 cut(s)	1227
BbsI	GAAGAC	2 cut(s)	53, 100
Bbv12I	GWGCWC	1 cut(s)	864
BbvI	GCAGC	3 cut(s)	439, 1386, 1878
BceAI	ACGGC	2 cut(s)	27, 180
BclI	TGATCA	3 cut(s)	519, 1663, 1737
BcnI	CCSGG	1 cut(s)	1190
BcoDI	GTCTC	1 cut(s)	526
BfaI	CTAG	5 cut(s)	330, 713, 762, 1004, 1230
BfmI	CTRYAG	1 cut(s)	453
BfoI	RGCGCY	1 cut(s)	453
BisI	GCNGC	6 cut(s)	55, 224, 453, 1356, 1375, 1867
BlsI	GCNGC	6 cut(s)	56, 225, 454, 1357, 1376, 1868
Bme1390I	CCNGG	1 cut(s)	1190
BmiI	GGNNCC	2 cut(s)	12, 1815
BmrFI	CCNGG	1 cut(s)	1190
BmrI	ACTGGG	2 cut(s)	16, 259
BmsI	GCATC	3 cut(s)	334, 454, 1562
BmuI	ACTGGG	2 cut(s)	16, 259
BpiI	GAAGAC	2 cut(s)	53, 100
BpuEI	CTTGAG	1 cut(s)	1298
BpuMI	CCSGG	1 cut(s)	1190
Bsa29I	ATCGAT	2 cut(s)	324, 1323
BsaJI	CCNNGG	2 cut(s)	55, 963
BsaWI	WCCGGW	1 cut(s)	976
Bsc4I	CCNNNNNNNGG	2 cut(s)	1219, 1294
Bse1I	ACTGG	4 cut(s)	11, 180, 254, 540
Bse3DI	GCAATG	3 cut(s)	726, 1881, 1886
BseCI	ATCGAT	2 cut(s)	324, 1323
BseDI	CCNNGG	2 cut(s)	55, 963
BseGI	GGATG	7 cut(s)	544, 744, 1272, 1303, 1602, 1606, 1662
BseLI	CCNNNNNNNGG	2 cut(s)	1219, 1294
BseMI	GCAATG	3 cut(s)	726, 1881, 1886
BseMII	CTCAG	4 cut(s)	296, 500, 521, 1131
BseNI	ACTGG	4 cut(s)	11, 180, 254, 540
BseRI	GAGGAG	1 cut(s)	154
BseXI	GCAGC	3 cut(s)	439, 1386, 1878
Bsh1236I	CGCG	2 cut(s)	57, 207
BshFI	GGCC	3 cut(s)	233, 760, 1358
BshVI	ATCGAT	2 cut(s)	324, 1323
BsiHKAI	GWGCWC	1 cut(s)	864
BsiSI	CCGG	2 cut(s)	977, 1190
BslFI	GGGAC	1 cut(s)	509
BslI	CCNNNNNNNGG	2 cut(s)	1219, 1294
BsmAI	GTCTC	1 cut(s)	526
BsmFI	GGGAC	1 cut(s)	509
BsnI	GGCC	3 cut(s)	233, 760, 1358
Bsp1286I	GDGCHC	2 cut(s)	864, 1227
Bsp143I	GATC	6 cut(s)	321, 519, 1192, 1320, 1663, 1737
Bsp68I	TCGCGA	1 cut(s)	207
BspACI	CCGC	6 cut(s)	29, 55, 57, 224, 390, 1355
BspANI	GGCC	3 cut(s)	233, 760, 1358
BspCNI	CTCAG	4 cut(s)	295, 499, 522, 1132
BspDI	ATCGAT	2 cut(s)	324, 1323
BspFNI	CGCG	2 cut(s)	57, 207
BspLI	GGNNCC	2 cut(s)	12, 1815
BspMAI	CTGCAG	1 cut(s)	457
BspPI	GGATC	1 cut(s)	1200
BsrBI	CCGCTC	1 cut(s)	1355
BsrDI	GCAATG	3 cut(s)	726, 1881, 1886
BsrI	ACTGG	4 cut(s)	11, 180, 254, 540
BssECI	CCNNGG	2 cut(s)	55, 963
BssMI	GATC	6 cut(s)	321, 519, 1192, 1320, 1663, 1737
Bst4CI	ACNGT	6 cut(s)	173, 307, 383, 506, 866, 1990
Bst6I	CTCTTC	1 cut(s)	740
BstAPI	GCANNNNNTGC	1 cut(s)	452
BstC8I	GCNNGC	4 cut(s)	390, 448, 799, 1242
BstDEI	CTNAG	7 cut(s)	154, 235, 282, 486, 530, 692, 1140
BstDSI	CCRYGG	1 cut(s)	55
BstENI	CCTNNNNNAGG	2 cut(s)	1217, 1292
BstF5I	GGATG	7 cut(s)	544, 744, 1272, 1303, 1602, 1606, 1662
BstFNI	CGCG	2 cut(s)	57, 207
BstH2I	RGCGCY	1 cut(s)	453
BstHHI	GCGC	1 cut(s)	452
BstKTI	GATC	6 cut(s)	324, 522, 1195, 1323, 1666, 1740
BstMAI	GTCTC	1 cut(s)	526
BstMBI	GATC	6 cut(s)	321, 519, 1192, 1320, 1663, 1737
BstMWI	GCNNNNNNNGC	3 cut(s)	452, 725, 1250
BstNSI	RCATGY	2 cut(s)	801, 1381
BstSCI	CCNGG	1 cut(s)	1188
BstSFI	CTRYAG	1 cut(s)	453
BstUI	CGCG	2 cut(s)	57, 207
BstV1I	GCAGC	3 cut(s)	439, 1386, 1878
BstV2I	GAAGAC	2 cut(s)	53, 100
Bsu15I	ATCGAT	2 cut(s)	324, 1323
BsuRI	GGCC	3 cut(s)	233, 760, 1358
BsuTUI	ATCGAT	2 cut(s)	324, 1323
BtgI	CCRYGG	1 cut(s)	55
BtgZI	GCGATG	1 cut(s)	1071
BtsCI	GGATG	7 cut(s)	544, 744, 1272, 1303, 1602, 1606, 1662
BtsIMutI	CAGTG	4 cut(s)	169, 862, 1563, 1995
BtuMI	TCGCGA	1 cut(s)	207
Cac8I	GCNNGC	4 cut(s)	390, 448, 799, 1242
CfoI	GCGC	1 cut(s)	452
Cfr42I	CCGCGG	1 cut(s)	58
ClaI	ATCGAT	2 cut(s)	324, 1323
Csp6I	GTAC	4 cut(s)	303, 337, 483, 1936
CspCI	CAANNNNNGTGG	4 cut(s)	904, 939, 1791, 1826
CviAII	CATG	8 cut(s)	314, 584, 781, 798, 1168, 1378, 1892, 1900
CviQI	GTAC	4 cut(s)	303, 337, 483, 1936
DdeI	CTNAG	7 cut(s)	154, 235, 282, 486, 530, 692, 1140
DpnI	GATC	6 cut(s)	323, 521, 1194, 1322, 1665, 1739
DpnII	GATC	6 cut(s)	321, 519, 1192, 1320, 1663, 1737
DrdI	GACNNNNNNGTC	1 cut(s)	104
DseDI	GACNNNNNNGTC	1 cut(s)	104
EaeI	YGGCCR	1 cut(s)	1356
Eam1104I	CTCTTC	1 cut(s)	740
EarI	CTCTTC	1 cut(s)	740
EciI	GGCGGA	1 cut(s)	18
Eco24I	GRGCYC	1 cut(s)	1227
Eco32I	GATATC	1 cut(s)	165
Eco47III	AGCGCT	1 cut(s)	451
EcoNI	CCTNNNNNAGG	2 cut(s)	1217, 1292
EcoRV	GATATC	1 cut(s)	165
EcoT22I	ATGCAT	1 cut(s)	469
EcoT38I	GRGCYC	1 cut(s)	1227
FaeI	CATG	8 cut(s)	317, 587, 784, 801, 1171, 1381, 1895, 1903
FaqI	GGGAC	1 cut(s)	509
FatI	CATG	8 cut(s)	313, 583, 780, 797, 1167, 1377, 1891, 1899
FauI	CCCGC	1 cut(s)	397
FauNDI	CATATG	1 cut(s)	643
FbaI	TGATCA	3 cut(s)	519, 1663, 1737
Fnu4HI	GCNGC	6 cut(s)	55, 224, 453, 1356, 1375, 1867
FokI	GGATG	7 cut(s)	551, 751, 1259, 1310, 1609, 1613, 1669
FriOI	GRGCYC	1 cut(s)	1227
Fsp4HI	GCNGC	6 cut(s)	55, 224, 453, 1356, 1375, 1867
FspBI	CTAG	5 cut(s)	330, 713, 762, 1004, 1230
GlaI	GCGC	1 cut(s)	451
GluI	GCNGC	6 cut(s)	55, 224, 453, 1356, 1375, 1867
HaeII	RGCGCY	1 cut(s)	453
HaeIII	GGCC	3 cut(s)	233, 760, 1358
HapII	CCGG	2 cut(s)	977, 1190
HhaI	GCGC	1 cut(s)	452
Hin1II	CATG	8 cut(s)	317, 587, 784, 801, 1171, 1381, 1895, 1903
Hin6I	GCGC	1 cut(s)	450
HinP1I	GCGC	1 cut(s)	450
HindIII	AAGCTT	2 cut(s)	16, 1146
HinfI	GANTC	6 cut(s)	293, 326, 364, 497, 556, 1583
HpaII	CCGG	2 cut(s)	977, 1190
HphI	GGTGA	8 cut(s)	75, 440, 529, 821, 1192, 1402, 1525, 1714
Hpy166II	GTNNAC	3 cut(s)	1099, 1394, 1938
Hpy188I	TCNGA	8 cut(s)	48, 97, 216, 502, 738, 859, 1165, 1470
Hpy188III	TCNNGA	9 cut(s)	206, 523, 1230, 1318, 1400, 1607, 1817, 1922, 1964
Hpy8I	GTNNAC	3 cut(s)	1099, 1394, 1938
Hpy99I	CGWCG	2 cut(s)	506, 1249
HpyAV	CCTTC	8 cut(s)	401, 425, 679, 823, 1124, 1439, 1489, 1783
HpyCH4III	ACNGT	6 cut(s)	173, 307, 383, 506, 866, 1990
HpyCH4IV	ACGT	1 cut(s)	1934
HpyF10VI	GCNNNNNNNGC	3 cut(s)	452, 725, 1250
HpyF3I	CTNAG	7 cut(s)	154, 235, 282, 486, 530, 692, 1140
HpySE526I	ACGT	1 cut(s)	1934
Hsp92II	CATG	8 cut(s)	317, 587, 784, 801, 1171, 1381, 1895, 1903
HspAI	GCGC	1 cut(s)	450
Ksp22I	TGATCA	3 cut(s)	519, 1663, 1737
KspI	CCGCGG	1 cut(s)	58
Kzo9I	GATC	6 cut(s)	321, 519, 1192, 1320, 1663, 1737
LmnI	GCTCC	2 cut(s)	794, 859
Lsp1109I	GCAGC	3 cut(s)	439, 1386, 1878
LweI	GCATC	3 cut(s)	334, 454, 1562
MaeI	CTAG	5 cut(s)	330, 713, 762, 1004, 1230
MaeII	ACGT	1 cut(s)	1934
MaeIII	GTNAC	6 cut(s)	708, 769, 868, 883, 1587, 1720
MalI	GATC	6 cut(s)	323, 521, 1194, 1322, 1665, 1739
MbiI	CCGCTC	1 cut(s)	1355
MboI	GATC	6 cut(s)	321, 519, 1192, 1320, 1663, 1737
MfeI	CAATTG	3 cut(s)	892, 1049, 1364
MhlI	GDGCHC	2 cut(s)	864, 1227
MlyI	GAGTC	2 cut(s)	373, 491
MmeI	TCCRAC	3 cut(s)	120, 525, 837
Mph1103I	ATGCAT	1 cut(s)	469
MseI	TTAA	5 cut(s)	126, 579, 591, 663, 777
MspA1I	CMGCKG	1 cut(s)	57
MspI	CCGG	2 cut(s)	977, 1190
MspR9I	CCNGG	1 cut(s)	1190
MunI	CAATTG	3 cut(s)	892, 1049, 1364
MvnI	CGCG	2 cut(s)	57, 207
MwoI	GCNNNNNNNGC	3 cut(s)	452, 725, 1250
NciI	CCSGG	1 cut(s)	1190
NdeI	CATATG	1 cut(s)	643
NdeII	GATC	6 cut(s)	321, 519, 1192, 1320, 1663, 1737
NlaIII	CATG	8 cut(s)	317, 587, 784, 801, 1171, 1381, 1895, 1903
NlaIV	GGNNCC	2 cut(s)	12, 1815
NmuCI	GTSAC	1 cut(s)	1720
NruI	TCGCGA	1 cut(s)	207
NsiI	ATGCAT	1 cut(s)	469
NspI	RCATGY	2 cut(s)	801, 1381
PaeI	GCATGC	1 cut(s)	801
PcsI	WCGNNNNNNNCGW	1 cut(s)	498
PfeI	GAWTC	4 cut(s)	293, 326, 556, 1583
PkrI	GCNGC	6 cut(s)	56, 225, 454, 1357, 1376, 1868
PleI	GAGTC	2 cut(s)	372, 491
PpsI	GAGTC	2 cut(s)	372, 491
PsiI	TTATAA	3 cut(s)	246, 1616, 1826
PspN4I	GGNNCC	2 cut(s)	12, 1815
PsrI	GAACNNNNNNTAC	2 cut(s)	693, 725
PstI	CTGCAG	1 cut(s)	457
RruI	TCGCGA	1 cut(s)	207
RsaI	GTAC	4 cut(s)	304, 338, 484, 1937
RsaNI	GTAC	4 cut(s)	303, 337, 483, 1936
SacII	CCGCGG	1 cut(s)	58
SaqAI	TTAA	5 cut(s)	126, 579, 591, 663, 777
SatI	GCNGC	6 cut(s)	55, 224, 453, 1356, 1375, 1867
Sau3AI	GATC	6 cut(s)	321, 519, 1192, 1320, 1663, 1737
SchI	GAGTC	2 cut(s)	373, 491
ScrFI	CCNGG	1 cut(s)	1190
SduI	GDGCHC	2 cut(s)	864, 1227
SfaNI	GCATC	3 cut(s)	334, 454, 1562
SfcI	CTRYAG	1 cut(s)	453
Sfr303I	CCGCGG	1 cut(s)	58
SgrBI	CCGCGG	1 cut(s)	58
SmlI	CTYRAG	1 cut(s)	1277
SmoI	CTYRAG	1 cut(s)	1277
SphI	GCATGC	1 cut(s)	801
SsiI	CCGC	6 cut(s)	29, 55, 57, 224, 390, 1355
SspMI	CTAG	5 cut(s)	330, 713, 762, 1004, 1230
StyD4I	CCNGG	1 cut(s)	1188
TaaI	ACNGT	6 cut(s)	173, 307, 383, 506, 866, 1990
TaiI	ACGT	1 cut(s)	1937
TaqI	TCGA	4 cut(s)	133, 324, 554, 1323
TaqII	GACCGA	1 cut(s)	977
TatI	WGTACW	2 cut(s)	302, 482
TauI	GCSGC	3 cut(s)	57, 226, 1358
TfiI	GAWTC	4 cut(s)	293, 326, 556, 1583
Tru1I	TTAA	5 cut(s)	126, 579, 591, 663, 777
Tru9I	TTAA	5 cut(s)	126, 579, 591, 663, 777
TscAI	CASTG	4 cut(s)	176, 869, 1563, 1995
TseFI	GTSAC	1 cut(s)	1720
TseI	GCWGC	3 cut(s)	452, 1374, 1866
Tsp45I	GTSAC	1 cut(s)	1720
TspGWI	ACGGA	1 cut(s)	1262
TspRI	CASTG	4 cut(s)	176, 869, 1563, 1995
XagI	CCTNNNNNAGG	2 cut(s)	1217, 1292
XapI	RAATTY	4 cut(s)	239, 1345, 1704, 1972
XbaI	TCTAGA	1 cut(s)	1229
XceI	RCATGY	2 cut(s)	801, 1381
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1366
XspI	CTAG	5 cut(s)	330, 713, 762, 1004, 1230
Zsp2I	ATGCAT	1 cut(s)	469

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.