Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0019132

Physical Location & Seq

Genomic Coordinates Reverse (-)

5369202 .. 5370990

Gene Structure 1789 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1057900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGAACTGGGTTCCAAGCTTCTTCTCTCCCCACAAAACAACCCGTCTTATTTGCCGCGGTCTTCACGCGAGTAAGCAATTCTCACCCCCAAACTCCGACAACATTGTCTTCAAAGCAATTTGTGTTAATCTTCGACAGAGGAGATGGAAGTTCTTAGACCAGATATCCCCCACTCTAACCAGTTCATTAGTCGGCCGTGTTGTTCGCGAGTTTCGGAACTCGCCGCAGTTGGCTTTAGAGTTTTACAACTGGGCTCGGAGGAACCAGAGTTGTTGTCCTCAATTTTTGGAATCTTCTTGGACTTTAATTCATGTTTTGATCGATTCTAGGAGGTACGATGATGCCCTGTCTCTTATGGAGAGTCTAATGGGGGAAGAGGGTTTGCACCCATTGGTTGTTTTGGAAGGGTTGGTTACTAGCTATGATGAAGGTTGTGGTTGTTCAAGCCCTGCGGTGTTTGATGCACTTGTTAGGGCTTGTACTCAGTTCGGGGCCACGGATGGTGCTTATGAGGTGATCAAGAAGCTGAGATTGGATGGTTGTTGGGTTTCGATTCACGCTTGGAATAACTTTCTTAACCATGTGATTAAGTTGAATGAGATTGATAGGTTTTGGAAGATGTATATGGAAATGCTTTCATATGGTTATGTTGAAAATGTTAATACTTTCAATTTGGTTATGTATGCTCTTTGTAAGGAGTGCAAGTTAACAGAAGCAATGTCGGAGGACTGTGTTACGCCCAATTCAGTTACTTACAACTGTATCATTAACGGGTTTTGCAAAATCGGTGGTTTATCGGTTGCAGAAGAAATTCGTGGTGAAATGACCGAGGCAGGTGTTGAGGCCAATTTGAGGACTTATGGTGCGATGAAATGGTGGAAAGGGTTCACCTACTCAATCCTCATCAAGGGGCTCTCTAGAAGTGGGCTTGTGACGGAAGCGGTTAGATTTCATAACCACATCCTTGAGAAGAACCTTGTCGAGGATGTTTTCTCCCACAATATCCTGATCGATTATATGTGCAAAAGCAACAATTTGAGAGCGGCCAAGCAATTGATGGGCAGCATGTTTGTTCGTGGTTTACTTCCTGACACTGTCACCTATGGCACTTTGATTGATGGGCACTGCAAAGAAGGGAGCATAGGAAGTGCTGTTCAGATTTATGAAAAACTGATAGAGGGGAAGAAAAAACCTAATTTGAGGATGGATGTTCTTGACATTGTAACCTATAACACAATGATAAACGGGTATTTCATCAGCGGGATGATTGATCAGGCGTTTGTTTTGGTTCGAGAAATGGAAGAGAGTGGAATTTCATTCAATATCGTCACCTACAATATATTGATCAACTTTTTGTGCAAGTTTGGGTGTATTCAACAAGCACAAGGACTAATGAAGGTAATGATTTCAAGGGGCATGGTTCCGGATTTTGTAACATACACCACTCTCATTACCAATTTGAACAAGAATTGCAGCCCGGAAGAAGTCGTTGCATTGCATGACTACATGGTGACTAAGGGAGTAATTCCTGATAGGCAAACGTACAAAGATATTGTCTTTCCATATCTTCTAGAAGAAAATTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

529

Amino Acids

60.01

Weight (kDa)

6.44

Isoelectric Point (pI)

45.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	184 - 232	2.1e-06	PPR repeat family
PPR_1	PF12854	244 - 276	4.3e-09	PPR repeat
PPR_2	PF13041	247 - 290	1e-09	PPR repeat family
PPR_2	PF13041	329 - 377	2.6e-13	PPR repeat family
PPR_1	PF12854	359 - 390	1.3e-09	PPR repeat
PPR_2	PF13041	363 - 399	3.6e-06	PPR repeat family
PPR_1	PF12854	405 - 434	2.4e-09	PPR repeat
PPR_3	PF13812	405 - 451	6.5e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	406 - 454	3.5e-17	PPR repeat family
PPR	PF01535	408 - 438	3.5e-08	PPR repeat
PPR_1	PF12854	437 - 468	2.9e-06	PPR repeat
PPR_2	PF13041	441 - 487	2.8e-12	PPR repeat family
PPR_2	PF13041	475 - 515	8.2e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11710 AT1G11710
malus_domestica	MD02G1262300.v1.1 MD07G1057900.v1.1 MD07G1059400.v1.1
prunus_persica	Prupe.2G064100_v2.0.a1
pyrus_communis	pycom02g22430 pycom07g04410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	824
AasI	GACNNNNNNGTC	1 cut(s)	104
Acc36I	ACCTGC	1 cut(s)	824
AccBSI	CCGCTC	1 cut(s)	1043
AccII	CGCG	3 cut(s)	57, 68, 207
AccIII	TCCGGA	1 cut(s)	1423
AciI	CCGC	7 cut(s)	55, 57, 224, 452, 941, 1043, 1260
AcoI	YGGCCR	2 cut(s)	193, 1044
AcsI	RAATTY	3 cut(s)	810, 1311, 1579
AfaI	GTAC	3 cut(s)	335, 481, 1544
AfiI	CCNNNNNNNGG	3 cut(s)	852, 907, 982
AgsI	TTSAA	9 cut(s)	112, 444, 595, 653, 670, 1321, 1376, 1410, 1462
AjuI	GAANNNNNNNTTGG	2 cut(s)	515, 547
AloI	GAACNNNNNNTCC	2 cut(s)	1137, 1169
AluBI	AGCT	3 cut(s)	18, 420, 526
AluI	AGCT	3 cut(s)	18, 420, 526
Alw26I	GTCTC	1 cut(s)	354
Aor13HI	TCCGGA	1 cut(s)	1423
AoxI	GGCC	4 cut(s)	193, 492, 843, 1044
ApeKI	GCWGC	2 cut(s)	1062, 1473
ApoI	RAATTY	3 cut(s)	810, 1311, 1579
ArsI	GACNNNNNNTTYG	2 cut(s)	1539, 1571
Asp700I	GAANNNNTTC	2 cut(s)	810, 884
AspS9I	GGNCC	1 cut(s)	492
AsuC2I	CCSGG	1 cut(s)	1478
AsuHPI	GGTGA	7 cut(s)	75, 526, 830, 880, 1090, 1321, 1522
BaeGI	GKGCMC	1 cut(s)	1125
BaeI	ACNNNNGTAYC	2 cut(s)	745, 778
BanII	GRGCYC	2 cut(s)	256, 915
BbsI	GAAGAC	2 cut(s)	53, 100
BbvI	GCAGC	2 cut(s)	1074, 1485
BccI	CCATC	6 cut(s)	138, 494, 530, 1051, 1112, 1198
BceAI	ACGGC	1 cut(s)	180
BclI	TGATCA	3 cut(s)	516, 1270, 1344
BcnI	CCSGG	1 cut(s)	1478
BcoDI	GTCTC	1 cut(s)	354
BfaI	CTAG	4 cut(s)	327, 417, 918, 1571
BfuAI	ACCTGC	1 cut(s)	824
BisI	GCNGC	5 cut(s)	55, 224, 1044, 1063, 1474
BlsI	GCNGC	5 cut(s)	56, 225, 1045, 1064, 1475
Bme1390I	CCNGG	1 cut(s)	1478
BmgT120I	GGNCC	1 cut(s)	492
BmiI	GGNNCC	4 cut(s)	12, 263, 493, 1422
BmrFI	CCNGG	1 cut(s)	1478
BmrI	ACTGGG	2 cut(s)	16, 259
BmsI	GCATC	2 cut(s)	331, 451
BmuI	ACTGGG	2 cut(s)	16, 259
BpiI	GAAGAC	2 cut(s)	53, 100
BpuEI	CTTGAG	1 cut(s)	986
BpuMI	CCSGG	1 cut(s)	1478
Bsa29I	ATCGAT	2 cut(s)	321, 1011
BsaJI	CCNNGG	3 cut(s)	55, 495, 828
BsaWI	WCCGGW	1 cut(s)	1423
BsaXI	ACNNNNNCTCC	2 cut(s)	689, 719
Bsc4I	CCNNNNNNNGG	3 cut(s)	852, 907, 982
Bse1I	ACTGG	3 cut(s)	11, 180, 254
Bse3DI	GCAATG	2 cut(s)	723, 1493
BseAI	TCCGGA	1 cut(s)	1423
BseCI	ATCGAT	2 cut(s)	321, 1011
BseDI	CCNNGG	3 cut(s)	55, 495, 828
BseGI	GGATG	7 cut(s)	505, 541, 960, 991, 1209, 1213, 1269
BseLI	CCNNNNNNNGG	3 cut(s)	852, 907, 982
BseMI	GCAATG	2 cut(s)	723, 1493
BseMII	CTCAG	2 cut(s)	497, 518
BseNI	ACTGG	3 cut(s)	11, 180, 254
BseRI	GAGGAG	1 cut(s)	154
BseSI	GKGCMC	1 cut(s)	1125
BseX3I	CGGCCG	1 cut(s)	193
BseXI	GCAGC	2 cut(s)	1074, 1485
Bsh1236I	CGCG	3 cut(s)	57, 68, 207
Bsh1285I	CGRYCG	1 cut(s)	196
BshFI	GGCC	4 cut(s)	195, 494, 845, 1046
BshVI	ATCGAT	2 cut(s)	321, 1011
BsiEI	CGRYCG	1 cut(s)	196
BsiSI	CCGG	2 cut(s)	1424, 1478
BslI	CCNNNNNNNGG	3 cut(s)	852, 907, 982
BsmAI	GTCTC	1 cut(s)	354
BsnI	GGCC	4 cut(s)	195, 494, 845, 1046
Bsp1286I	GDGCHC	3 cut(s)	256, 915, 1125
Bsp13I	TCCGGA	1 cut(s)	1423
Bsp143I	GATC	5 cut(s)	318, 516, 1008, 1270, 1344
Bsp68I	TCGCGA	1 cut(s)	207
BspACI	CCGC	7 cut(s)	55, 57, 224, 452, 941, 1043, 1260
BspANI	GGCC	4 cut(s)	195, 494, 845, 1046
BspCNI	CTCAG	2 cut(s)	496, 519
BspDI	ATCGAT	2 cut(s)	321, 1011
BspEI	TCCGGA	1 cut(s)	1423
BspFNI	CGCG	3 cut(s)	57, 68, 207
BspLI	GGNNCC	4 cut(s)	12, 263, 493, 1422
BspMI	ACCTGC	1 cut(s)	824
BsrBI	CCGCTC	1 cut(s)	1043
BsrDI	GCAATG	2 cut(s)	723, 1493
BsrI	ACTGG	3 cut(s)	11, 180, 254
BssECI	CCNNGG	3 cut(s)	55, 495, 828
BssMI	GATC	5 cut(s)	318, 516, 1008, 1270, 1344
Bst4CI	ACNGT	3 cut(s)	731, 761, 1096
Bst6I	CTCTTC	2 cut(s)	369, 1296
BstDEI	CTNAG	4 cut(s)	154, 483, 527, 1515
BstDSI	CCRYGG	2 cut(s)	55, 495
BstENI	CCTNNNNNAGG	2 cut(s)	905, 980
BstF5I	GGATG	7 cut(s)	505, 541, 960, 991, 1209, 1213, 1269
BstFNI	CGCG	3 cut(s)	57, 68, 207
BstKTI	GATC	5 cut(s)	321, 519, 1011, 1273, 1347
BstMAI	GTCTC	1 cut(s)	354
BstMBI	GATC	5 cut(s)	318, 516, 1008, 1270, 1344
BstMCI	CGRYCG	1 cut(s)	196
BstNSI	RCATGY	1 cut(s)	1069
BstSCI	CCNGG	1 cut(s)	1476
BstSLI	GKGCMC	1 cut(s)	1125
BstUI	CGCG	3 cut(s)	57, 68, 207
BstV1I	GCAGC	2 cut(s)	1074, 1485
BstV2I	GAAGAC	2 cut(s)	53, 100
BstZI	CGGCCG	1 cut(s)	193
Bsu15I	ATCGAT	2 cut(s)	321, 1011
BsuRI	GGCC	4 cut(s)	195, 494, 845, 1046
BsuTUI	ATCGAT	2 cut(s)	321, 1011
BtgI	CCRYGG	2 cut(s)	55, 495
BtgZI	GCGATG	1 cut(s)	881
BtsCI	GGATG	7 cut(s)	505, 541, 960, 991, 1209, 1213, 1269
BtsI	GCAGTG	1 cut(s)	1123
BtsIMutI	CAGTG	2 cut(s)	1092, 1123
BtuMI	TCGCGA	1 cut(s)	207
BveI	ACCTGC	1 cut(s)	824
Cfr13I	GGNCC	1 cut(s)	492
Cfr42I	CCGCGG	1 cut(s)	58
ClaI	ATCGAT	2 cut(s)	321, 1011
Csp6I	GTAC	3 cut(s)	334, 480, 1543
CspCI	CAANNNNNGTGG	2 cut(s)	769, 804
CviAII	CATG	6 cut(s)	311, 581, 1066, 1417, 1499, 1507
CviQI	GTAC	3 cut(s)	334, 480, 1543
DdeI	CTNAG	4 cut(s)	154, 483, 527, 1515
DpnI	GATC	5 cut(s)	320, 518, 1010, 1272, 1346
DpnII	GATC	5 cut(s)	318, 516, 1008, 1270, 1344
DrdI	GACNNNNNNGTC	1 cut(s)	104
DseDI	GACNNNNNNGTC	1 cut(s)	104
EaeI	YGGCCR	2 cut(s)	193, 1044
EagI	CGGCCG	1 cut(s)	193
Eam1104I	CTCTTC	2 cut(s)	369, 1296
EarI	CTCTTC	2 cut(s)	369, 1296
EclXI	CGGCCG	1 cut(s)	193
Eco24I	GRGCYC	2 cut(s)	256, 915
Eco32I	GATATC	1 cut(s)	165
Eco52I	CGGCCG	1 cut(s)	193
EcoNI	CCTNNNNNAGG	2 cut(s)	905, 980
EcoRV	GATATC	1 cut(s)	165
EcoT38I	GRGCYC	2 cut(s)	256, 915
FaeI	CATG	6 cut(s)	314, 584, 1069, 1420, 1502, 1510
FatI	CATG	6 cut(s)	310, 580, 1065, 1416, 1498, 1506
FauI	CCCGC	1 cut(s)	1253
FauNDI	CATATG	1 cut(s)	640
FbaI	TGATCA	3 cut(s)	516, 1270, 1344
Fnu4HI	GCNGC	5 cut(s)	55, 224, 1044, 1063, 1474
FokI	GGATG	7 cut(s)	512, 548, 947, 998, 1216, 1220, 1276
FriOI	GRGCYC	2 cut(s)	256, 915
Fsp4HI	GCNGC	5 cut(s)	55, 224, 1044, 1063, 1474
FspBI	CTAG	4 cut(s)	327, 417, 918, 1571
GluI	GCNGC	5 cut(s)	55, 224, 1044, 1063, 1474
HaeIII	GGCC	4 cut(s)	195, 494, 845, 1046
HapII	CCGG	2 cut(s)	1424, 1478
Hin1II	CATG	6 cut(s)	314, 584, 1069, 1420, 1502, 1510
HincII	GTYRAC	1 cut(s)	708
HindII	GTYRAC	1 cut(s)	708
HindIII	AAGCTT	1 cut(s)	16
HinfI	GANTC	4 cut(s)	290, 323, 361, 553
HpaI	GTTAAC	1 cut(s)	708
HpaII	CCGG	2 cut(s)	1424, 1478
HphI	GGTGA	7 cut(s)	75, 526, 830, 880, 1090, 1321, 1522
Hpy166II	GTNNAC	3 cut(s)	708, 888, 1082
Hpy188I	TCNGA	5 cut(s)	97, 216, 258, 724, 1158
Hpy8I	GTNNAC	3 cut(s)	708, 888, 1082
HpyAV	CCTTC	4 cut(s)	398, 422, 1127, 1390
HpyCH4III	ACNGT	3 cut(s)	731, 761, 1096
HpyCH4IV	ACGT	1 cut(s)	1541
HpyF3I	CTNAG	4 cut(s)	154, 483, 527, 1515
HpySE526I	ACGT	1 cut(s)	1541
Hsp92II	CATG	6 cut(s)	314, 584, 1069, 1420, 1502, 1510
Kpn2I	TCCGGA	1 cut(s)	1423
Ksp22I	TGATCA	3 cut(s)	516, 1270, 1344
KspAI	GTTAAC	1 cut(s)	708
KspI	CCGCGG	1 cut(s)	58
Kzo9I	GATC	5 cut(s)	318, 516, 1008, 1270, 1344
LmnI	GCTCC	1 cut(s)	1137
Lsp1109I	GCAGC	2 cut(s)	1074, 1485
LweI	GCATC	2 cut(s)	331, 451
MaeI	CTAG	4 cut(s)	327, 417, 918, 1571
MaeII	ACGT	1 cut(s)	1541
MaeIII	GTNAC	9 cut(s)	412, 733, 748, 931, 1096, 1222, 1327, 1432, 1510
MalI	GATC	5 cut(s)	320, 518, 1010, 1272, 1346
MbiI	CCGCTC	1 cut(s)	1043
MboI	GATC	5 cut(s)	318, 516, 1008, 1270, 1344
MfeI	CAATTG	1 cut(s)	1052
MhlI	GDGCHC	3 cut(s)	256, 915, 1125
MlyI	GAGTC	1 cut(s)	370
MmeI	TCCRAC	2 cut(s)	120, 702
MroI	TCCGGA	1 cut(s)	1423
MroXI	GAANNNNTTC	2 cut(s)	810, 884
MseI	TTAA	7 cut(s)	126, 305, 576, 588, 660, 707, 768
MspA1I	CMGCKG	2 cut(s)	57, 1260
MspI	CCGG	2 cut(s)	1424, 1478
MspR9I	CCNGG	1 cut(s)	1478
MunI	CAATTG	1 cut(s)	1052
MvnI	CGCG	3 cut(s)	57, 68, 207
NciI	CCSGG	1 cut(s)	1478
NdeI	CATATG	1 cut(s)	640
NdeII	GATC	5 cut(s)	318, 516, 1008, 1270, 1344
NlaIII	CATG	6 cut(s)	314, 584, 1069, 1420, 1502, 1510
NlaIV	GGNNCC	4 cut(s)	12, 263, 493, 1422
NmuCI	GTSAC	4 cut(s)	931, 1096, 1327, 1510
NruI	TCGCGA	1 cut(s)	207
NspI	RCATGY	1 cut(s)	1069
PaqCI	CACCTGC	1 cut(s)	824
PdmI	GAANNNNTTC	2 cut(s)	810, 884
PfeI	GAWTC	3 cut(s)	290, 323, 553
PflFI	GACNNNGTC	1 cut(s)	1094
PkrI	GCNGC	5 cut(s)	56, 225, 1045, 1064, 1475
PleI	GAGTC	1 cut(s)	369
PpsI	GAGTC	1 cut(s)	369
PspN4I	GGNNCC	4 cut(s)	12, 263, 493, 1422
PspPI	GGNCC	1 cut(s)	492
PsyI	GACNNNGTC	1 cut(s)	1094
RruI	TCGCGA	1 cut(s)	207
RsaI	GTAC	3 cut(s)	335, 481, 1544
RsaNI	GTAC	3 cut(s)	334, 480, 1543
SacII	CCGCGG	1 cut(s)	58
SaqAI	TTAA	7 cut(s)	126, 305, 576, 588, 660, 707, 768
SatI	GCNGC	5 cut(s)	55, 224, 1044, 1063, 1474
Sau3AI	GATC	5 cut(s)	318, 516, 1008, 1270, 1344
Sau96I	GGNCC	1 cut(s)	492
SchI	GAGTC	1 cut(s)	370
ScrFI	CCNGG	1 cut(s)	1478
SduI	GDGCHC	3 cut(s)	256, 915, 1125
SfaNI	GCATC	2 cut(s)	331, 451
Sfr303I	CCGCGG	1 cut(s)	58
SgrBI	CCGCGG	1 cut(s)	58
SmlI	CTYRAG	1 cut(s)	965
SmoI	CTYRAG	1 cut(s)	965
SsiI	CCGC	7 cut(s)	55, 57, 224, 452, 941, 1043, 1260
SspMI	CTAG	4 cut(s)	327, 417, 918, 1571
StyD4I	CCNGG	1 cut(s)	1476
TaaI	ACNGT	3 cut(s)	731, 761, 1096
TaiI	ACGT	1 cut(s)	1544
TaqI	TCGA	6 cut(s)	133, 321, 551, 981, 1011, 1291
TaqII	GACCGA	1 cut(s)	842
TatI	WGTACW	1 cut(s)	479
TauI	GCSGC	3 cut(s)	57, 226, 1046
TfiI	GAWTC	3 cut(s)	290, 323, 553
Tru1I	TTAA	7 cut(s)	126, 305, 576, 588, 660, 707, 768
Tru9I	TTAA	7 cut(s)	126, 305, 576, 588, 660, 707, 768
TscAI	CASTG	2 cut(s)	1099, 1130
TseFI	GTSAC	4 cut(s)	931, 1096, 1327, 1510
TseI	GCWGC	2 cut(s)	1062, 1473
Tsp45I	GTSAC	4 cut(s)	931, 1096, 1327, 1510
TspGWI	ACGGA	2 cut(s)	512, 950
TspRI	CASTG	2 cut(s)	1099, 1130
Tth111I	GACNNNGTC	1 cut(s)	1094
XagI	CCTNNNNNAGG	2 cut(s)	905, 980
XapI	RAATTY	3 cut(s)	810, 1311, 1579
XbaI	TCTAGA	2 cut(s)	917, 1570
XceI	RCATGY	1 cut(s)	1069
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1054
XmnI	GAANNNNTTC	2 cut(s)	810, 884
XspI	CTAG	4 cut(s)	327, 417, 918, 1571

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.