MD02G1283700.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014500
Physical Location & Seq
Reverse (-)
34024342 .. 34025301
960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1283700.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGAAGTGAAGCTCGATACGGTTCCGTCCCTGGTCTCCAAGCTCAGCTCTGTCTCCTCCCAAACCCGAGCCGACGCCCTCAAAGAGCTTCGCCTCATCACCAAGCTCGAACCGGACAGCCGTCCCTTCGTTGCCGAATCTGGCGCCATCCCTTACTTGTCCGAAACCCTCTTCGACTCCTCCCCTTACCTCCAAGACGACGCCGCCGCCACCCTCCTAAACCTCTCCATCTCCTGCCGCGACTCCCTCATCTCCACACAAGGCCTCCTCGATTCTCTCTCCCACTCTCTCCGCCAACACGGCTCCCCTTCCTCCTCCCCCTCCGCTGTCCAGTCTTCCGCCGCCACCCTTCACAGCCTCCTCGTCGTTGACGACTACCGCCCCATCATCGGCTCCAAGCGCGACATCGTCTACTCCCTCATCGACATCGTCAAGTGCCCTAACTCGCCGCCGCGGTCTGTGAAGGACTCGCTGAAGGCGCTGTTCGGGATCGCTCTCTACGCGCCCAACCGCATCGCCTTGGTGGAGCTCGGGGCGGTTCCCGCGCTTTTTACATTGGTAGTGAAGGACGGGCGGGTGGGGATCGTGGAGGACGCCACGGCGGTGATCTCGCAGGTGGCGGGGTGCGAGGAGAGCGAGGAGGCGTTTCAGAGGGTTTCCGGGATAAGGGTTTTGGCGGATTTGCTGGATCTCTCGACCGGATCGAGCTTGAGGAGCAAGGAGAACGCGGTGGGGGCGCTGCTCAATTTGGCCACGTGCGGCGGAGATTGGGGGCTGAGGGAGGTGAGGGAGGAGGGGATGGGAGTGGTGGATGGGGTTGCTGACGTAGCGGAGAATGGCGGCATAAAAGCGAAGAGCAAGGCAGTTGCGCTGCTGAAGGTGATTGATGGCGGGAGCGGATGCATTGCTAGCGTTTTTAGAGATCCACGGTTTGATTCTTTACTAAACCAAACTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm

Protein Analysis

320

Amino Acids

33.66

Weight (kDa)

5.05

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 6 - 188 5.3e-07 ARMC5
ARM_PUB PF25598 10 - 296 6.9e-16 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08315
fragaria_vesca FvH4_7g03360 FvH4_7g03360
malus_domestica MD02G1283700.v1.1 MD07G1049200.v1.1
prunus_persica Prupe.2G038900_v2.0.a1
pyrus_communis pycom02g24170
rosa_chinensis RchiOBHm_Chr1g0325681
rosa_laevigata RLG00000030112
rosa_multiflora Rmu_sc0004714.1_g000002
rosa_roxburghii Rroxscaffold_4G00324480
rosa_rugosa Rorug01G0053700
rosa_samantha Rh1AG069500 Rh1BG056700 Rh1CG069800 Rh1DG074400
rosa_wichuraiana Rw1G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 604
Acc36I ACCTGC 1 cut(s) 604
AccB1I GGYRCC 1 cut(s) 143
AccBSI CCGCTC 1 cut(s) 897
AccI GTMKAC 1 cut(s) 411
AccII CGCG 6 cut(s) 240, 402, 454, 503, 545, 728
AclWI GGATC 5 cut(s) 497, 590, 696, 709, 917
AcoI YGGCCR 1 cut(s) 750
AcuI CTGAAG 2 cut(s) 494, 896
AcvI CACGTG 1 cut(s) 756
AcyI GRCGYC 4 cut(s) 75, 144, 201, 594
AfiI CCNNNNNNNGG 2 cut(s) 389, 666
AjnI CCWGG 1 cut(s) 30
AleI CACNNNNGTG 1 cut(s) 602
AluBI AGCT 7 cut(s) 13, 43, 48, 88, 106, 529, 708
AluI AGCT 7 cut(s) 13, 43, 48, 88, 106, 529, 708
Alw21I GWGCWC 1 cut(s) 531
Alw26I GTCTC 2 cut(s) 40, 58
AlwI GGATC 5 cut(s) 497, 590, 696, 709, 917
Ama87I CYCGRG 2 cut(s) 66, 530
AoxI GGCC 2 cut(s) 262, 750
ApeKI GCWGC 2 cut(s) 739, 871
AspLEI GCGC 7 cut(s) 146, 402, 481, 505, 547, 739, 871
AsuC2I CCSGG 1 cut(s) 661
AsuHPI GGTGA 4 cut(s) 91, 616, 796, 892
AsuNHI GCTAGC 1 cut(s) 908
AvaI CYCGRG 2 cut(s) 66, 530
BaeGI GKGCMC 1 cut(s) 440
BalI TGGCCA 1 cut(s) 752
BanI GGYRCC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 531
BbrPI CACGTG 1 cut(s) 756
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 531
BbvCI CCTCAGC 1 cut(s) 776
BbvI GCAGC 2 cut(s) 726, 858
BccI CCATC 6 cut(s) 155, 236, 392, 793, 806, 881
BceAI ACGGC 3 cut(s) 105, 316, 615
BciT130I CCWGG 1 cut(s) 32
BcnI CCSGG 1 cut(s) 661
BcoDI GTCTC 2 cut(s) 40, 58
BfaI CTAG 1 cut(s) 909
BfoI RGCGCY 3 cut(s) 147, 482, 740
BfuAI ACCTGC 1 cut(s) 604
BglI GCCNNNNNGGC 1 cut(s) 300
BlpI GCTNAGC 1 cut(s) 44
Bme1390I CCNGG 2 cut(s) 32, 661
BmeT110I CYCGRG 2 cut(s) 66, 530
BmiI GGNNCC 5 cut(s) 24, 145, 304, 394, 540
BmrFI CCNGG 2 cut(s) 32, 661
BmsI GCATC 2 cut(s) 522, 890
BmtI GCTAGC 1 cut(s) 912
BoxI GACNNNNGTC 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 776
Bpu1102I GCTNAGC 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 730
BpuMI CCSGG 1 cut(s) 661
BsaAI YACGTR 1 cut(s) 756
BsaHI GRCGYC 4 cut(s) 75, 144, 201, 594
BsaI GGTCTC 1 cut(s) 40
BsaJI CCNNGG 5 cut(s) 30, 452, 519, 597, 926
BsaWI WCCGGW 2 cut(s) 112, 698
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 666
Bse1I ACTGG 1 cut(s) 331
Bse3DI GCAATG 1 cut(s) 903
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 5 cut(s) 30, 452, 519, 597, 926
BseGI GGATG 4 cut(s) 147, 804, 817, 905
BseLI CCNNNNNNNGG 2 cut(s) 389, 666
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 2 cut(s) 58, 767
BseNI ACTGG 1 cut(s) 331
BseRI GAGGAG 9 cut(s) 46, 169, 257, 304, 350, 644, 653, 727, 806
BseSI GKGCMC 1 cut(s) 440
BseXI GCAGC 2 cut(s) 726, 858
Bsh1236I CGCG 6 cut(s) 240, 402, 454, 503, 545, 728
Bsh1285I CGRYCG 1 cut(s) 699
BshFI GGCC 2 cut(s) 264, 752
BshNI GGYRCC 1 cut(s) 143
BsiEI CGRYCG 1 cut(s) 699
BsiHKAI GWGCWC 1 cut(s) 531
BsiHKCI CYCGRG 2 cut(s) 66, 530
BsiSI CCGG 3 cut(s) 113, 660, 699
BslFI GGGAC 2 cut(s) 13, 108
BslI CCNNNNNNNGG 2 cut(s) 389, 666
BsmAI GTCTC 2 cut(s) 40, 58
BsmFI GGGAC 2 cut(s) 13, 108
BsnI GGCC 2 cut(s) 264, 752
Bso31I GGTCTC 1 cut(s) 40
BsoBI CYCGRG 2 cut(s) 66, 530
Bsp1286I GDGCHC 2 cut(s) 440, 531
Bsp143I GATC 6 cut(s) 489, 582, 606, 688, 701, 922
Bsp1720I GCTNAGC 1 cut(s) 44
BspANI GGCC 2 cut(s) 264, 752
BspCNI CTCAG 2 cut(s) 57, 768
BspFNI CGCG 6 cut(s) 240, 402, 454, 503, 545, 728
BspLI GGNNCC 5 cut(s) 24, 145, 304, 394, 540
BspMI ACCTGC 1 cut(s) 604
BspOI GCTAGC 1 cut(s) 912
BspPI GGATC 5 cut(s) 497, 590, 696, 709, 917
BspQI GCTCTTC 1 cut(s) 848
BspT107I GGYRCC 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 40
BsrBI CCGCTC 1 cut(s) 897
BsrDI GCAATG 1 cut(s) 903
BsrI ACTGG 1 cut(s) 331
BssECI CCNNGG 5 cut(s) 30, 452, 519, 597, 926
BssMI GATC 6 cut(s) 489, 582, 606, 688, 701, 922
BssNI GRCGYC 4 cut(s) 75, 144, 201, 594
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 22, 930
Bst6I CTCTTC 2 cut(s) 176, 848
BstACI GRCGYC 4 cut(s) 75, 144, 201, 594
BstBAI YACGTR 1 cut(s) 756
BstC8I GCNNGC 1 cut(s) 910
BstDEI CTNAG 2 cut(s) 44, 776
BstDSI CCRYGG 3 cut(s) 452, 597, 926
BstF5I GGATG 4 cut(s) 147, 804, 817, 905
BstFNI CGCG 6 cut(s) 240, 402, 454, 503, 545, 728
BstH2I RGCGCY 3 cut(s) 147, 482, 740
BstHHI GCGC 7 cut(s) 146, 402, 481, 505, 547, 739, 871
BstKTI GATC 6 cut(s) 492, 585, 609, 691, 704, 925
BstMAI GTCTC 2 cut(s) 40, 58
BstMBI GATC 6 cut(s) 489, 582, 606, 688, 701, 922
BstMCI CGRYCG 1 cut(s) 699
BstNI CCWGG 1 cut(s) 32
BstPAI GACNNNNGTC 1 cut(s) 120
BstSCI CCNGG 2 cut(s) 30, 659
BstSLI GKGCMC 1 cut(s) 440
BstUI CGCG 6 cut(s) 240, 402, 454, 503, 545, 728
BstV1I GCAGC 2 cut(s) 726, 858
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 2 cut(s) 688, 922
BstYI RGATCY 2 cut(s) 688, 922
BsuRI GGCC 2 cut(s) 264, 752
BtgI CCRYGG 3 cut(s) 452, 597, 926
BtgZI GCGATG 1 cut(s) 499
BtsCI GGATG 4 cut(s) 147, 804, 817, 905
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 1 cut(s) 910
CfoI GCGC 7 cut(s) 146, 402, 481, 505, 547, 739, 871
Cfr42I CCGCGG 1 cut(s) 455
CseI GACGC 3 cut(s) 83, 209, 602
DdeI CTNAG 2 cut(s) 44, 776
DinI GGCGCC 1 cut(s) 145
DpnI GATC 6 cut(s) 491, 584, 608, 690, 703, 924
DpnII GATC 6 cut(s) 489, 582, 606, 688, 701, 922
EaeI YGGCCR 1 cut(s) 750
Eam1104I CTCTTC 2 cut(s) 176, 848
EarI CTCTTC 2 cut(s) 176, 848
EciI GGCGGA 4 cut(s) 281, 328, 692, 777
Ecl136II GAGCTC 1 cut(s) 529
Eco130I CCWWGG 1 cut(s) 519
Eco147I AGGCCT 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 531
Eco31I GGTCTC 1 cut(s) 40
Eco53kI GAGCTC 1 cut(s) 529
Eco57I CTGAAG 2 cut(s) 494, 896
Eco72I CACGTG 1 cut(s) 756
Eco88I CYCGRG 2 cut(s) 66, 530
EcoICRI GAGCTC 1 cut(s) 529
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 1 cut(s) 519
EcoT22I ATGCAT 1 cut(s) 905
EcoT38I GRGCYC 1 cut(s) 531
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 519
FaiI YATR 1 cut(s) 845
FaqI GGGAC 2 cut(s) 13, 108
FauI CCCGC 4 cut(s) 550, 567, 613, 884
FblI GTMKAC 1 cut(s) 411
FokI GGATG 4 cut(s) 134, 811, 824, 912
FriOI GRGCYC 1 cut(s) 531
FspBI CTAG 1 cut(s) 909
GlaI GCGC 7 cut(s) 145, 401, 480, 504, 546, 738, 870
HaeII RGCGCY 3 cut(s) 147, 482, 740
HaeIII GGCC 2 cut(s) 264, 752
HapII CCGG 3 cut(s) 113, 660, 699
HgaI GACGC 3 cut(s) 83, 209, 602
HhaI GCGC 7 cut(s) 146, 402, 481, 505, 547, 739, 871
Hin1I GRCGYC 4 cut(s) 75, 144, 201, 594
Hin6I GCGC 7 cut(s) 144, 400, 479, 503, 545, 737, 869
HinP1I GCGC 7 cut(s) 144, 400, 479, 503, 545, 737, 869
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 6 cut(s) 137, 176, 242, 272, 467, 935
HpaII CCGG 3 cut(s) 113, 660, 699
HphI GGTGA 4 cut(s) 91, 616, 796, 892
Hpy166II GTNNAC 2 cut(s) 370, 412
Hpy188I TCNGA 2 cut(s) 163, 651
Hpy188III TCNNGA 2 cut(s) 487, 694
Hpy8I GTNNAC 2 cut(s) 370, 412
Hpy99I CGWCG 3 cut(s) 77, 203, 368
HpyAV CCTTC 7 cut(s) 136, 318, 359, 457, 469, 559, 871
HpyCH4III ACNGT 2 cut(s) 22, 930
HpyCH4IV ACGT 2 cut(s) 755, 825
HpyCH4V TGCA 1 cut(s) 903
HpyF3I CTNAG 2 cut(s) 44, 776
HpySE526I ACGT 2 cut(s) 755, 825
Hsp92I GRCGYC 4 cut(s) 75, 144, 201, 594
HspAI GCGC 7 cut(s) 144, 400, 479, 503, 545, 737, 869
KasI GGCGCC 1 cut(s) 143
KspI CCGCGG 1 cut(s) 455
Kzo9I GATC 6 cut(s) 489, 582, 606, 688, 701, 922
LguI GCTCTTC 1 cut(s) 848
LmnI GCTCC 5 cut(s) 308, 398, 526, 714, 894
Lsp1109I GCAGC 2 cut(s) 726, 858
LweI GCATC 2 cut(s) 522, 890
MaeI CTAG 1 cut(s) 909
MaeII ACGT 2 cut(s) 755, 825
MalI GATC 6 cut(s) 491, 584, 608, 690, 703, 924
MbiI CCGCTC 1 cut(s) 897
MboI GATC 6 cut(s) 489, 582, 606, 688, 701, 922
MboII GAAGA 3 cut(s) 163, 327, 865
MflI RGATCY 2 cut(s) 688, 922
MhlI GDGCHC 2 cut(s) 440, 531
MlsI TGGCCA 1 cut(s) 752
MluCI AATT 1 cut(s) 745
MluNI TGGCCA 1 cut(s) 752
Mly113I GGCGCC 1 cut(s) 144
MlyI GAGTC 3 cut(s) 170, 236, 461
Mox20I TGGCCA 1 cut(s) 752
Mph1103I ATGCAT 1 cut(s) 905
MscI TGGCCA 1 cut(s) 752
MslI CAYNNNNRTG 1 cut(s) 602
Msp20I TGGCCA 1 cut(s) 752
MspA1I CMGCKG 2 cut(s) 326, 454
MspI CCGG 3 cut(s) 113, 660, 699
MspR9I CCNGG 2 cut(s) 32, 661
MvaI CCWGG 1 cut(s) 32
MvnI CGCG 6 cut(s) 240, 402, 454, 503, 545, 728
NarI GGCGCC 1 cut(s) 144
NciI CCSGG 1 cut(s) 661
NdeII GATC 6 cut(s) 489, 582, 606, 688, 701, 922
NheI GCTAGC 1 cut(s) 908
NlaIV GGNNCC 5 cut(s) 24, 145, 304, 394, 540
NsiI ATGCAT 1 cut(s) 905
OliI CACNNNNGTG 1 cut(s) 602
PaqCI CACCTGC 1 cut(s) 604
PceI AGGCCT 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 848
PcsI WCGNNNNNNNCGW 2 cut(s) 369, 701
PfeI GAWTC 3 cut(s) 137, 272, 935
PflFI GACNNNGTC 2 cut(s) 407, 428
PfoI TCCNGGA 1 cut(s) 659
PleI GAGTC 3 cut(s) 170, 236, 461
PluTI GGCGCC 1 cut(s) 147
PmaCI CACGTG 1 cut(s) 756
PmlI CACGTG 1 cut(s) 756
PpsI GAGTC 3 cut(s) 170, 236, 461
Ppu21I YACGTR 1 cut(s) 756
PshAI GACNNNNGTC 1 cut(s) 120
Psp124BI GAGCTC 1 cut(s) 531
Psp6I CCWGG 1 cut(s) 30
PspCI CACGTG 1 cut(s) 756
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 5 cut(s) 24, 145, 304, 394, 540
PsuI RGATCY 2 cut(s) 688, 922
PsyI GACNNNGTC 2 cut(s) 407, 428
RseI CAYNNNNRTG 1 cut(s) 602
SacI GAGCTC 1 cut(s) 531
SacII CCGCGG 1 cut(s) 455
SapI GCTCTTC 1 cut(s) 848
Sau3AI GATC 6 cut(s) 489, 582, 606, 688, 701, 922
SchI GAGTC 3 cut(s) 170, 236, 461
ScrFI CCNGG 2 cut(s) 32, 661
SduI GDGCHC 2 cut(s) 440, 531
SfaNI GCATC 2 cut(s) 522, 890
SfoI GGCGCC 1 cut(s) 145
Sfr303I CCGCGG 1 cut(s) 455
SgrBI CCGCGG 1 cut(s) 455
SmiMI CAYNNNNRTG 1 cut(s) 602
SmlI CTYRAG 1 cut(s) 709
SmoI CTYRAG 1 cut(s) 709
Sse9I AATT 1 cut(s) 745
SseBI AGGCCT 1 cut(s) 264
SspDI GGCGCC 1 cut(s) 143
SspMI CTAG 1 cut(s) 909
SstI GAGCTC 1 cut(s) 531
StuI AGGCCT 1 cut(s) 264
StyD4I CCNGG 2 cut(s) 30, 659
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 2 cut(s) 22, 930
TaiI ACGT 2 cut(s) 758, 828
TaqI TCGA 7 cut(s) 15, 108, 174, 270, 423, 695, 704
TasI AATT 1 cut(s) 745
TauI GCSGC 8 cut(s) 206, 209, 240, 344, 451, 454, 762, 843
TfiI GAWTC 3 cut(s) 137, 272, 935
TseI GCWGC 2 cut(s) 739, 871
TspGWI ACGGA 1 cut(s) 15
Tth111I GACNNNGTC 2 cut(s) 407, 428
XmiI GTMKAC 1 cut(s) 411
XspI CTAG 1 cut(s) 909
Zsp2I ATGCAT 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.