Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0013706

Physical Location & Seq

Genomic Coordinates Reverse (-)

37279454 .. 37284590

Gene Structure 5137 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1318400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2229 bp

ATGAACGATATAAAAAGCACAGCAGGCTCCAATCCACAATCCAACCCCATAAACGTCACCATGAAAGGTGGCGCTGCCACTGGCGGCACTCCTAGTTTCAACAACAACAACATTACTACTACTAGTGACCAAACGACGACGTCTACGTCCTCCTGGGGCACGTGGGAGGAGCTCCTGCTAGCATGCGCCGTCAAGCGCTACGGCATCCACGACTGGGACACCGTCGCCATGGAACTCCAAGCCAAGACCTCTCTCCCTCCCCTCCTCACCACCGCCTCCAACTGCAAGCTCAAGTACCTCGACCTCCGCCGTCGTTTCCGCTCCCGCCACCAAAACGACGACGTCCCTCCAGACGCCGACGACAATCACCACGATCCTCATAACAATAATACTAACAATATTCCCTGGCTCGACGACCTCCGCAAACTCCGCGTCGCCCAGCTCCGTCAAGAGCTCCATCGCTACGACGTCTCTATCGTGTCGTTGCAGTTGAAGGTGAAGAGGTTGGAGGAGGAGAGAGAAGAGAGCTTCAAAGACGACGACCCCAAACCAGATCTGGAGGACCAGCAGCAGCAGGACTCCAATCGACACAGATCAGAAAACGACAAAACAGTTCAAGGCGAACCGGACGGGTCGAAACCGGACCACGAGAACCAGTCGGTGAACGGCTCCAATTCGACCTGCTCCAAAAACCTGGACCACGACAACAAGTCCGGTCCAGAAGATCGCGCTCTCTCTGAACCGGGCCTGCTCCCAGCCGCTTCCGCTGAACCTTCCAAAAGGACGGGGAGTGGGCAGTCGGATTCGGACTCGGACAAAGGAAGCTCGGAAACGGTGGCAAAGAACCGGAAGGAACCGGGCCGGCAAGGGAAAGGTGGGGGCGATTCGGCGGATAAGATGAGGGAGAGCAGCGAGGCGCAGAGTTCGGTTAGCTTAACGAAAAAATGGAACGGCAAACGACGCCGTATAATGGAGGTCCCAGAGGAGCAGCAGCAGCACAATAACGGAGATGTATCGTTGGTGAAATCACAGCCGTTGGTTGAGGTGTTGCAGTTGATACGTGCGCAAGAGCATGGCTCGTTGTTCGAGCGACGGCTTCCGGCCCAGGAAAGCGATAAGTACAAGAGCATTGTCCAGCAGCATGTAGACCTGGAAACTATACAAACAAAGCTCCGCAAGGACGCCTACTCATCCTGCTCTCTCTCCTTCTACCGAGACCTGCTCCTCCTCTTCAACAATGCCGTCGTCTTCTTCCCCAAATCCTCCCTCGAGTCAAGGACTGCTCACCAACTCCGCCGCCTCGTCTTAAATGAAATGAAGAAGAAGAATCTCATCACACGGCCCAACCACGACCCTTCATCTGAGCAATCAGACTCGGTAGTTCCTGCACCCGCCACAAGTCAGCAACCCAAACGCCCTGAGCAGCTTGAAGGATCAGATTCCTTGCTTGCCAAGCAAAAGTCTACTGCACCCATCATTGTCTGCCGCAAAAGGAGCTCCATTTCGTCAAAACCTTCCGCGGCTGCTTCTAGCTTTGGTCAGACTCTCAAAGGAGATGAGAAGAAGAAAGCAGCAGCTTTTGATCTTAAACCGTCCATTAATGCCACCAAGGCCTCTTCTGATGATCATGCAGCAGCTGAAAAGCGTGGCAGTTCAGTACTAGTAAAGAGTGCTGCTGCTGCTGCTAAAGCCAAAGACAACCCCGTGATCACTGGAACCAGAAGCTCGAGAAGGACGAGTATGAGTACTGCTCATCACGAGAATAATAATGGCAGTGCTTCCAAGAAGAAACAAAGTGCGAGCCTGGCAAGTTTGAAAGCCGAAACTCCCAAACCGGATAAGAAGGGGAAAACGGAGGCAGCAGGGTCGGACAAAAAGCGTAGTGCAGCAGCTGATTTCTTGAAGAGAATAAAGCGCAATTCACCTGCGGACACACTGAAGAGTGGTGGCACTGGCACCGGCACTAGTTCTAGATCAGGTGGAGAGCAAAAGAGGAGGGGAAGTGGTGGTAAGGTTGACAAAGTTAAAGAAAGGGTGTTAAGGCAAAGTGGCGATAAGAAGAAACAAGCCAAGGAAGAAAGCAGCAGTCCATCCAAGCGAAGTGTAGGAAGGCCGCCCAAGAAGGCGGCTGCTGAGGCTGCAATTGTAGCAGCTTCACCAAAGCGGCCACGGGAGAGTGGAGGACAGCAAGAGGTTTCTAAGCGGCCTAAAAAACGGTCTAGAAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Bromodomain Myb_DNA-binding

Protein Analysis

743

Amino Acids

81.18

Weight (kDa)

9.75

Isoelectric Point (pI)

62.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	51 - 101	8.8e-06	Myb-like DNA-binding domain
Bromodomain	PF00439	348 - 428	1.4e-09	Bromodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013706)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g00180 FvH4_7g00180
malus_domestica	MD02G1318400.v1.1 MD07G1002900.v1.1 MD07G1247400.v1.1
prunus_persica	Prupe.2G004300_v2.0.a1
pyrus_communis	pycom02g26660 pycom07g00290
rosa_chinensis	RchiOBHm_Chr1g0313431
rosa_laevigata	RLG00000030797
rosa_roxburghii	Rroxscaffold_4G00332190
rosa_rugosa	RorugPtG0005000.1
rosa_samantha	Rh1AG003600 Rh1BG000500 Rh1CG001100 Rh1CG007900 Rh1DG003400
rosa_wichuraiana	Rw1G000230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1933
AasI	GACNNNNNNGTC	1 cut(s)	145
AatII	GACGTC	3 cut(s)	143, 345, 471
Acc16I	TGCGCA	1 cut(s)	1065
Acc36I	ACCTGC	3 cut(s)	689, 1227, 1933
AccB1I	GGYRCC	1 cut(s)	1955
AccBSI	CCGCTC	1 cut(s)	321
AccI	GTMKAC	3 cut(s)	143, 1146, 1463
AccII	CGCG	3 cut(s)	432, 729, 1520
AclWI	GGATC	2 cut(s)	368, 1441
AcoI	YGGCCR	1 cut(s)	2165
AcuI	CTGAAG	1 cut(s)	1958
AcvI	CACGTG	1 cut(s)	162
AcyI	GRCGYC	6 cut(s)	140, 342, 354, 468, 961, 1182
AfaI	GTAC	4 cut(s)	296, 1121, 1659, 1747
AfeI	AGCGCT	1 cut(s)	197
AfiI	CCNNNNNNNGG	2 cut(s)	214, 970
AgsI	TTSAA	8 cut(s)	100, 493, 532, 617, 1234, 1430, 1816, 1903
AhdI	GACNNNNNGTC	1 cut(s)	709
AhlI	ACTAGT	3 cut(s)	122, 1660, 1964
AjnI	CCWGG	6 cut(s)	152, 404, 693, 1104, 1149, 1803
Alw21I	GWGCWC	3 cut(s)	174, 456, 1499
Alw26I	GTCTC	2 cut(s)	475, 1209
AlwI	GGATC	2 cut(s)	368, 1441
AlwNI	CAGNNNCTG	2 cut(s)	1637, 1892
Ama87I	CYCGRG	2 cut(s)	1268, 1726
Aor51HI	AGCGCT	1 cut(s)	197
AoxI	GGCC	8 cut(s)	745, 859, 1101, 1340, 1611, 2111, 2165, 2204
ArsI	GACNNNNNNTTYG	2 cut(s)	417, 449
AseI	ATTAAT	1 cut(s)	1599
AspLEI	GCGC	7 cut(s)	74, 188, 198, 731, 919, 1066, 1917
AspS9I	GGNCC	9 cut(s)	562, 643, 697, 716, 745, 859, 976, 1102, 1341
AsuC2I	CCSGG	2 cut(s)	744, 858
AsuHPI	GGTGA	9 cut(s)	49, 259, 359, 508, 673, 1033, 1277, 1914, 2148
AsuNHI	GCTAGC	1 cut(s)	178
AvaI	CYCGRG	2 cut(s)	1268, 1726
AvaII	GGWCC	5 cut(s)	562, 643, 697, 716, 976
BaeGI	GKGCMC	1 cut(s)	161
BanI	GGYRCC	1 cut(s)	1955
BanII	GRGCYC	3 cut(s)	174, 456, 1499
BauI	CACGAG	2 cut(s)	647, 1757
BbrPI	CACGTG	1 cut(s)	162
BbsI	GAAGAC	1 cut(s)	1240
Bbv12I	GWGCWC	3 cut(s)	174, 456, 1499
BbvCI	CCTCAGC	1 cut(s)	2133
BccI	CCATC	3 cut(s)	465, 1481, 2098
BcgI	CGANNNNNNTGC	2 cut(s)	1848, 1882
BciT130I	CCWGG	6 cut(s)	154, 406, 695, 1106, 1151, 1805
BclI	TGATCA	2 cut(s)	1624, 1707
BcnI	CCSGG	2 cut(s)	744, 858
BcoDI	GTCTC	2 cut(s)	475, 1209
BcuI	ACTAGT	3 cut(s)	122, 1660, 1964
BfaI	CTAG	8 cut(s)	93, 123, 179, 1530, 1661, 1965, 1971, 2220
BfoI	RGCGCY	2 cut(s)	75, 199
BfuAI	ACCTGC	3 cut(s)	689, 1227, 1933
BglI	GCCNNNNNGGC	1 cut(s)	1610
BglII	AGATCT	1 cut(s)	553
BmcAI	AGTACT	2 cut(s)	1659, 1747
Bme1390I	CCNGG	8 cut(s)	154, 406, 695, 744, 858, 1106, 1151, 1805
Bme18I	GGWCC	5 cut(s)	562, 643, 697, 716, 976
BmeRI	GACNNNNNGTC	1 cut(s)	709
BmeT110I	CYCGRG	2 cut(s)	1268, 1726
BmgT120I	GGNCC	9 cut(s)	562, 643, 697, 716, 745, 859, 976, 1102, 1341
BmiI	GGNNCC	6 cut(s)	28, 670, 855, 978, 1717, 1957
BmrFI	CCNGG	8 cut(s)	154, 406, 695, 744, 858, 1106, 1151, 1805
BmrI	ACTGGG	1 cut(s)	223
BmsI	GCATC	1 cut(s)	213
BmtI	GCTAGC	1 cut(s)	182
BmuI	ACTGGG	1 cut(s)	223
BpiI	GAAGAC	1 cut(s)	1240
BplI	GAGNNNNNCTC	6 cut(s)	1061, 1093, 1206, 1238, 1735, 1767
BpmI	CTGGAG	2 cut(s)	333, 578
Bpu10I	CCTNAGC	2 cut(s)	1419, 2133
BpuEI	CTTGAG	1 cut(s)	275
BpuMI	CCSGG	2 cut(s)	744, 858
BsaAI	YACGTR	2 cut(s)	162, 1061
BsaHI	GRCGYC	6 cut(s)	140, 342, 354, 468, 961, 1182
BsaI	GGTCTC	1 cut(s)	1209
BsaJI	CCNNGG	8 cut(s)	153, 228, 404, 1104, 1518, 1608, 2070, 2168
BsaWI	WCCGGW	5 cut(s)	625, 640, 713, 846, 1834
BsaXI	ACNNNNNCTCC	2 cut(s)	1987, 2017
Bsc4I	CCNNNNNNNGG	2 cut(s)	214, 970
Bse118I	RCCGGY	2 cut(s)	861, 1958
Bse1I	ACTGG	5 cut(s)	85, 218, 655, 1717, 1957
BseBI	CCWGG	6 cut(s)	154, 406, 695, 1106, 1151, 1805
BseDI	CCNNGG	8 cut(s)	153, 228, 404, 1104, 1518, 1608, 2070, 2168
BseGI	GGATG	3 cut(s)	204, 1190, 2090
BseLI	CCNNNNNNNGG	2 cut(s)	214, 970
BseMII	CTCAG	3 cut(s)	1353, 1410, 2124
BseNI	ACTGG	5 cut(s)	85, 218, 655, 1717, 1957
BseRI	GAGGAG	8 cut(s)	182, 254, 524, 527, 998, 1214, 1217, 2008
BseSI	GKGCMC	1 cut(s)	161
BseYI	CCCAGC	2 cut(s)	438, 754
BsgI	GTGCAG	3 cut(s)	1371, 1452, 1905
Bsh1236I	CGCG	3 cut(s)	432, 729, 1520
BshFI	GGCC	8 cut(s)	747, 861, 1103, 1342, 1613, 2113, 2167, 2206
BshNI	GGYRCC	1 cut(s)	1955
BsiHKAI	GWGCWC	3 cut(s)	174, 456, 1499
BsiHKCI	CYCGRG	2 cut(s)	1268, 1726
BslFI	GGGAC	3 cut(s)	230, 329, 962
BslI	CCNNNNNNNGG	2 cut(s)	214, 970
BsmAI	GTCTC	2 cut(s)	475, 1209
BsmBI	CGTCTC	1 cut(s)	475
BsmFI	GGGAC	3 cut(s)	230, 329, 962
BsnI	GGCC	8 cut(s)	747, 861, 1103, 1342, 1613, 2113, 2167, 2206
Bso31I	GGTCTC	1 cut(s)	1209
BsoBI	CYCGRG	2 cut(s)	1268, 1726
Bsp1286I	GDGCHC	4 cut(s)	161, 174, 456, 1499
Bsp143I	GATC	9 cut(s)	373, 553, 593, 724, 1433, 1582, 1624, 1707, 1973
Bsp19I	CCATGG	1 cut(s)	228
BspANI	GGCC	8 cut(s)	747, 861, 1103, 1342, 1613, 2113, 2167, 2206
BspCNI	CTCAG	3 cut(s)	1354, 1411, 2125
BspFNI	CGCG	3 cut(s)	432, 729, 1520
BspLI	GGNNCC	6 cut(s)	28, 670, 855, 978, 1717, 1957
BspMI	ACCTGC	3 cut(s)	689, 1227, 1933
BspOI	GCTAGC	1 cut(s)	182
BspPI	GGATC	2 cut(s)	368, 1441
BspT107I	GGYRCC	1 cut(s)	1955
BspTNI	GGTCTC	1 cut(s)	1209
BsrBI	CCGCTC	1 cut(s)	321
BsrFI	RCCGGY	2 cut(s)	861, 1958
BsrI	ACTGG	5 cut(s)	85, 218, 655, 1717, 1957
BssAI	RCCGGY	2 cut(s)	861, 1958
BssECI	CCNNGG	8 cut(s)	153, 228, 404, 1104, 1518, 1608, 2070, 2168
BssMI	GATC	9 cut(s)	373, 553, 593, 724, 1433, 1582, 1624, 1707, 1973
BssNI	GRCGYC	6 cut(s)	140, 342, 354, 468, 961, 1182
BssSI	CACGAG	2 cut(s)	647, 1757
BssT1I	CCWWGG	3 cut(s)	228, 1608, 2070
Bst2BI	CACGAG	2 cut(s)	647, 1757
Bst2UI	CCWGG	6 cut(s)	154, 406, 695, 1106, 1151, 1805
Bst4CI	ACNGT	5 cut(s)	223, 613, 835, 1593, 2217
Bst6I	CTCTTC	6 cut(s)	494, 516, 1235, 1621, 1898, 1934
BstACI	GRCGYC	6 cut(s)	140, 342, 354, 468, 961, 1182
BstBAI	YACGTR	2 cut(s)	162, 1061
BstC8I	GCNNGC	8 cut(s)	25, 180, 184, 287, 749, 863, 1449, 1801
BstDEI	CTNAG	4 cut(s)	1362, 1419, 2133, 2199
BstDSI	CCRYGG	3 cut(s)	228, 1518, 2168
BstF5I	GGATG	3 cut(s)	204, 1190, 2090
BstFNI	CGCG	3 cut(s)	432, 729, 1520
BstH2I	RGCGCY	2 cut(s)	75, 199
BstHHI	GCGC	7 cut(s)	74, 188, 198, 731, 919, 1066, 1917
BstKTI	GATC	9 cut(s)	376, 556, 596, 727, 1436, 1585, 1627, 1710, 1976
BstMAI	GTCTC	2 cut(s)	475, 1209
BstMBI	GATC	9 cut(s)	373, 553, 593, 724, 1433, 1582, 1624, 1707, 1973
BstNI	CCWGG	6 cut(s)	154, 406, 695, 1106, 1151, 1805
BstNSI	RCATGY	2 cut(s)	186, 1145
BstSCI	CCNGG	8 cut(s)	152, 404, 693, 742, 856, 1104, 1149, 1803
BstSLI	GKGCMC	1 cut(s)	161
BstUI	CGCG	3 cut(s)	432, 729, 1520
BstV2I	GAAGAC	1 cut(s)	1240
BstX2I	RGATCY	1 cut(s)	553
BstXI	CCANNNNNNTGG	1 cut(s)	694
BstYI	RGATCY	1 cut(s)	553
BsuRI	GGCC	8 cut(s)	747, 861, 1103, 1342, 1613, 2113, 2167, 2206
BtgI	CCRYGG	3 cut(s)	228, 1518, 2168
BtgZI	GCGATG	1 cut(s)	443
BtsCI	GGATG	3 cut(s)	204, 1190, 2090
BtsI	GCAGTG	1 cut(s)	1780
BtsIMutI	CAGTG	5 cut(s)	78, 1710, 1780, 1934, 1950
BveI	ACCTGC	3 cut(s)	689, 1227, 1933
Cac8I	GCNNGC	8 cut(s)	25, 180, 184, 287, 749, 863, 1449, 1801
CaiI	CAGNNNCTG	2 cut(s)	1637, 1892
CfoI	GCGC	7 cut(s)	74, 188, 198, 731, 919, 1066, 1917
Cfr10I	RCCGGY	2 cut(s)	861, 1958
Cfr13I	GGNCC	9 cut(s)	562, 643, 697, 716, 745, 859, 976, 1102, 1341
Cfr42I	CCGCGG	1 cut(s)	1521
CseI	GACGC	4 cut(s)	362, 421, 969, 1190
Csp6I	GTAC	4 cut(s)	295, 1120, 1658, 1746
CviAII	CATG	6 cut(s)	61, 183, 229, 1073, 1142, 1628
CviQI	GTAC	4 cut(s)	295, 1120, 1658, 1746
DdeI	CTNAG	4 cut(s)	1362, 1419, 2133, 2199
DpnI	GATC	9 cut(s)	375, 555, 595, 726, 1435, 1584, 1626, 1709, 1975
DpnII	GATC	9 cut(s)	373, 553, 593, 724, 1433, 1582, 1624, 1707, 1973
DrdI	GACNNNNNNGTC	1 cut(s)	145
DriI	GACNNNNNGTC	1 cut(s)	709
DseDI	GACNNNNNNGTC	1 cut(s)	145
EaeI	YGGCCR	1 cut(s)	2165
Eam1104I	CTCTTC	6 cut(s)	494, 516, 1235, 1621, 1898, 1934
Eam1105I	GACNNNNNGTC	1 cut(s)	709
EarI	CTCTTC	6 cut(s)	494, 516, 1235, 1621, 1898, 1934
EciI	GGCGGA	3 cut(s)	296, 905, 1283
Ecl136II	GAGCTC	3 cut(s)	172, 454, 1497
Eco130I	CCWWGG	3 cut(s)	228, 1608, 2070
Eco147I	AGGCCT	1 cut(s)	1613
Eco24I	GRGCYC	3 cut(s)	174, 456, 1499
Eco31I	GGTCTC	1 cut(s)	1209
Eco47I	GGWCC	5 cut(s)	562, 643, 697, 716, 976
Eco47III	AGCGCT	1 cut(s)	197
Eco53kI	GAGCTC	3 cut(s)	172, 454, 1497
Eco57I	CTGAAG	1 cut(s)	1958
Eco72I	CACGTG	1 cut(s)	162
Eco88I	CYCGRG	2 cut(s)	1268, 1726
EcoICRI	GAGCTC	3 cut(s)	172, 454, 1497
EcoO109I	RGGNCCY	1 cut(s)	976
EcoRII	CCWGG	6 cut(s)	152, 404, 693, 1104, 1149, 1803
EcoT14I	CCWWGG	3 cut(s)	228, 1608, 2070
EcoT38I	GRGCYC	3 cut(s)	174, 456, 1499
ErhI	CCWWGG	3 cut(s)	228, 1608, 2070
Esp3I	CGTCTC	1 cut(s)	475
FaeI	CATG	6 cut(s)	64, 186, 232, 1076, 1145, 1631
FaqI	GGGAC	3 cut(s)	230, 329, 962
FatI	CATG	6 cut(s)	60, 182, 228, 1072, 1141, 1627
FauI	CCCGC	2 cut(s)	332, 1399
FbaI	TGATCA	2 cut(s)	1624, 1707
FblI	GTMKAC	3 cut(s)	143, 1146, 1463
FokI	GGATG	3 cut(s)	191, 1177, 2077
FriOI	GRGCYC	3 cut(s)	174, 456, 1499
FspBI	CTAG	8 cut(s)	93, 123, 179, 1530, 1661, 1965, 1971, 2220
FspI	TGCGCA	1 cut(s)	1065
GlaI	GCGC	7 cut(s)	73, 187, 197, 730, 918, 1065, 1916
GsaI	CCCAGC	2 cut(s)	442, 758
GsuI	CTGGAG	2 cut(s)	333, 578
HaeII	RGCGCY	2 cut(s)	75, 199
HaeIII	GGCC	8 cut(s)	747, 861, 1103, 1342, 1613, 2113, 2167, 2206
HgaI	GACGC	4 cut(s)	362, 421, 969, 1190
HhaI	GCGC	7 cut(s)	74, 188, 198, 731, 919, 1066, 1917
Hin1I	GRCGYC	6 cut(s)	140, 342, 354, 468, 961, 1182
Hin1II	CATG	6 cut(s)	64, 186, 232, 1076, 1145, 1631
Hin6I	GCGC	7 cut(s)	72, 186, 196, 729, 917, 1064, 1915
HinP1I	GCGC	7 cut(s)	72, 186, 196, 729, 917, 1064, 1915
HincII	GTYRAC	1 cut(s)	2017
HindII	GTYRAC	1 cut(s)	2017
HinfI	GANTC	9 cut(s)	578, 803, 809, 884, 1271, 1327, 1373, 1439, 1543
HphI	GGTGA	9 cut(s)	49, 259, 359, 508, 673, 1033, 1277, 1914, 2148
Hpy166II	GTNNAC	5 cut(s)	144, 664, 1147, 1464, 2017
Hpy188III	TCNNGA	9 cut(s)	350, 449, 557, 719, 1728, 1757, 1900, 1971, 2220
Hpy8I	GTNNAC	5 cut(s)	144, 664, 1147, 1464, 2017
HpyCH4III	ACNGT	5 cut(s)	223, 613, 835, 1593, 2217
HpyCH4IV	ACGT	7 cut(s)	54, 140, 146, 161, 342, 468, 1060
HpyCH4V	TGCA	8 cut(s)	285, 487, 1051, 1388, 1469, 1631, 1886, 2141
HpyF3I	CTNAG	4 cut(s)	1362, 1419, 2133, 2199
HpySE526I	ACGT	7 cut(s)	54, 140, 146, 161, 342, 468, 1060
Hsp92I	GRCGYC	6 cut(s)	140, 342, 354, 468, 961, 1182
Hsp92II	CATG	6 cut(s)	64, 186, 232, 1076, 1145, 1631
HspAI	GCGC	7 cut(s)	72, 186, 196, 729, 917, 1064, 1915
KroI	GCCGGC	1 cut(s)	861
KroNI	GCCGGC	1 cut(s)	863
Ksp22I	TGATCA	2 cut(s)	1624, 1707
KspI	CCGCGG	1 cut(s)	1521
Kzo9I	GATC	9 cut(s)	373, 553, 593, 724, 1433, 1582, 1624, 1707, 1973
LweI	GCATC	1 cut(s)	213
MaeI	CTAG	8 cut(s)	93, 123, 179, 1530, 1661, 1965, 1971, 2220
MaeII	ACGT	7 cut(s)	54, 140, 146, 161, 342, 468, 1060
MaeIII	GTNAC	2 cut(s)	55, 125
MalI	GATC	9 cut(s)	375, 555, 595, 726, 1435, 1584, 1626, 1709, 1975
MbiI	CCGCTC	1 cut(s)	321
MboI	GATC	9 cut(s)	373, 553, 593, 724, 1433, 1582, 1624, 1707, 1973
MfeI	CAATTG	1 cut(s)	2142
MflI	RGATCY	1 cut(s)	553
MhlI	GDGCHC	4 cut(s)	161, 174, 456, 1499
MluCI	AATT	3 cut(s)	673, 1918, 2142
MlyI	GAGTC	5 cut(s)	572, 803, 1280, 1367, 1537
MmeI	TCCRAC	5 cut(s)	66, 303, 486, 780, 1848
MroNI	GCCGGC	1 cut(s)	861
MseI	TTAA	6 cut(s)	935, 1307, 1587, 1599, 2025, 2039
MspA1I	CMGCKG	4 cut(s)	767, 1520, 1637, 1892
MspR9I	CCNGG	8 cut(s)	154, 406, 695, 744, 858, 1106, 1151, 1805
MunI	CAATTG	1 cut(s)	2142
MvaI	CCWGG	6 cut(s)	154, 406, 695, 1106, 1151, 1805
MvnI	CGCG	3 cut(s)	432, 729, 1520
NaeI	GCCGGC	1 cut(s)	863
NciI	CCSGG	2 cut(s)	744, 858
NcoI	CCATGG	1 cut(s)	228
NdeII	GATC	9 cut(s)	373, 553, 593, 724, 1433, 1582, 1624, 1707, 1973
NgoMIV	GCCGGC	1 cut(s)	861
NheI	GCTAGC	1 cut(s)	178
NlaIII	CATG	6 cut(s)	64, 186, 232, 1076, 1145, 1631
NlaIV	GGNNCC	6 cut(s)	28, 670, 855, 978, 1717, 1957
NmuCI	GTSAC	2 cut(s)	55, 125
NsbI	TGCGCA	1 cut(s)	1065
NspI	RCATGY	2 cut(s)	186, 1145
PaeI	GCATGC	1 cut(s)	186
PaeR7I	CTCGAG	2 cut(s)	1268, 1726
PaqCI	CACCTGC	1 cut(s)	1933
PceI	AGGCCT	1 cut(s)	1613
PcsI	WCGNNNNNNNCGW	4 cut(s)	143, 207, 474, 1733
PdiI	GCCGGC	1 cut(s)	863
PfeI	GAWTC	4 cut(s)	803, 884, 1327, 1439
PflFI	GACNNNGTC	3 cut(s)	139, 221, 341
PleI	GAGTC	5 cut(s)	572, 803, 1279, 1367, 1537
PmaCI	CACGTG	1 cut(s)	162
PmlI	CACGTG	1 cut(s)	162
PpsI	GAGTC	5 cut(s)	572, 803, 1279, 1367, 1537
Ppu21I	YACGTR	2 cut(s)	162, 1061
PpuMI	RGGWCCY	1 cut(s)	976
PshBI	ATTAAT	1 cut(s)	1599
Psp124BI	GAGCTC	3 cut(s)	174, 456, 1499
Psp5II	RGGWCCY	1 cut(s)	976
Psp6I	CCWGG	6 cut(s)	152, 404, 693, 1104, 1149, 1803
PspCI	CACGTG	1 cut(s)	162
PspFI	CCCAGC	2 cut(s)	438, 754
PspGI	CCWGG	6 cut(s)	152, 404, 693, 1104, 1149, 1803
PspN4I	GGNNCC	6 cut(s)	28, 670, 855, 978, 1717, 1957
PspPI	GGNCC	9 cut(s)	562, 643, 697, 716, 745, 859, 976, 1102, 1341
PspPPI	RGGWCCY	1 cut(s)	976
PspXI	VCTCGAGB	1 cut(s)	1268
PstNI	CAGNNNCTG	2 cut(s)	1637, 1892
PsuI	RGATCY	1 cut(s)	553
PsyI	GACNNNGTC	3 cut(s)	139, 221, 341
PvuII	CAGCTG	2 cut(s)	1637, 1892
RsaI	GTAC	4 cut(s)	296, 1121, 1659, 1747
RsaNI	GTAC	4 cut(s)	295, 1120, 1658, 1746
SacI	GAGCTC	3 cut(s)	174, 456, 1499
SacII	CCGCGG	1 cut(s)	1521
SaqAI	TTAA	6 cut(s)	935, 1307, 1587, 1599, 2025, 2039
Sau3AI	GATC	9 cut(s)	373, 553, 593, 724, 1433, 1582, 1624, 1707, 1973
Sau96I	GGNCC	9 cut(s)	562, 643, 697, 716, 745, 859, 976, 1102, 1341
ScaI	AGTACT	2 cut(s)	1659, 1747
SchI	GAGTC	5 cut(s)	572, 803, 1280, 1367, 1537
ScrFI	CCNGG	8 cut(s)	154, 406, 695, 744, 858, 1106, 1151, 1805
SduI	GDGCHC	4 cut(s)	161, 174, 456, 1499
SfaNI	GCATC	1 cut(s)	213
Sfr274I	CTCGAG	2 cut(s)	1268, 1726
Sfr303I	CCGCGG	1 cut(s)	1521
SgrBI	CCGCGG	1 cut(s)	1521
SinI	GGWCC	5 cut(s)	562, 643, 697, 716, 976
SlaI	CTCGAG	2 cut(s)	1268, 1726
SmlI	CTYRAG	3 cut(s)	290, 1268, 1726
SmoI	CTYRAG	3 cut(s)	290, 1268, 1726
SpeI	ACTAGT	3 cut(s)	122, 1660, 1964
SphI	GCATGC	1 cut(s)	186
Sse9I	AATT	3 cut(s)	673, 1918, 2142
SseBI	AGGCCT	1 cut(s)	1613
SspI	AATATT	1 cut(s)	400
SspMI	CTAG	8 cut(s)	93, 123, 179, 1530, 1661, 1965, 1971, 2220
SstI	GAGCTC	3 cut(s)	174, 456, 1499
StuI	AGGCCT	1 cut(s)	1613
StyD4I	CCNGG	8 cut(s)	152, 404, 693, 742, 856, 1104, 1149, 1803
StyI	CCWWGG	3 cut(s)	228, 1608, 2070
TaaI	ACNGT	5 cut(s)	223, 613, 835, 1593, 2217
TaiI	ACGT	7 cut(s)	57, 143, 149, 164, 345, 471, 1063
TaqI	TCGA	8 cut(s)	300, 411, 586, 635, 677, 1086, 1269, 1727
TasI	AATT	3 cut(s)	673, 1918, 2142
TatI	WGTACW	3 cut(s)	1119, 1657, 1745
TauI	GCSGC	9 cut(s)	87, 761, 1299, 1488, 1523, 2116, 2129, 2167, 2206
TfiI	GAWTC	4 cut(s)	803, 884, 1327, 1439
Tru1I	TTAA	6 cut(s)	935, 1307, 1587, 1599, 2025, 2039
Tru9I	TTAA	6 cut(s)	935, 1307, 1587, 1599, 2025, 2039
TscAI	CASTG	5 cut(s)	85, 1717, 1780, 1941, 1957
TseFI	GTSAC	2 cut(s)	55, 125
Tsp45I	GTSAC	2 cut(s)	55, 125
TspDTI	ATGAA	5 cut(s)	17, 77, 1326, 1331, 1347
TspGWI	ACGGA	3 cut(s)	434, 1020, 1868
TspRI	CASTG	5 cut(s)	85, 1717, 1780, 1941, 1957
Tth111I	GACNNNGTC	3 cut(s)	139, 221, 341
VpaK11BI	GGWCC	5 cut(s)	562, 643, 697, 716, 976
VspI	ATTAAT	1 cut(s)	1599
XbaI	TCTAGA	2 cut(s)	1970, 2219
XceI	RCATGY	2 cut(s)	186, 1145
XcmI	CCANNNNNNNNNTGG	1 cut(s)	553
XhoI	CTCGAG	2 cut(s)	1268, 1726
XmiI	GTMKAC	3 cut(s)	143, 1146, 1463
XspI	CTAG	8 cut(s)	93, 123, 179, 1530, 1661, 1965, 1971, 2220
ZraI	GACGTC	3 cut(s)	141, 343, 469
ZrmI	AGTACT	2 cut(s)	1659, 1747

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.