Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005951

Physical Location & Seq

Genomic Coordinates Reverse (-)

1622207 .. 1625684

Gene Structure 3478 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1021000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2325 bp

ATGCAGAAGCCTCCACCGTCTCAAGATTTCGCTCTGAAAGAGACCTCGCCAAACATTGGCGCGGGCTCTATCACGGGCGACAAGCTCTCATGCACCTATGACCTTGTAGAACGGATGCAATACCTTTACGTTCACGTGGTGAAAGCAAAGGATTTGCCTGCAAAAGATGTCACTGGTAGTTCTGATCCCTATGTTGAAGTAAAACTCGGAAACTATAAGGGAGTTACTAAGCATTTCGAGAAGAAGTCTAACCCTGAATGGAATCAGGTGTTTGCTTTCTCCAAAGAACGTCTTCAAGCTTCAGTTTTGGAAGTTGTGGTGAAAGATAAGGATGTTGTGCTGGATGATTTCATGGGCAGGATTATGATGGATCTCATTGATATCCCGAAACGCGAACCACCAGATAGCCCATTGGCACCACAATGGTATAGGCTGGAGGACAGGAAGGGGGATAAGGTCAAGGGAGAGCTGATGCTGGCAGTTTGGATGGGAACTCAAGCAGACGAGGCATTTCCTGATGCATGGCATTCTGATGCTGCAACAGTTGGGCCTGAGGGTGTTAAGAACATTCGATCGAAGGTATACCTCTCCCCCAAGCTTTGGTATGTGAGGGTTAATGTTATTGAAGCTCAGGACTTGCTACCAAATGACAAGAGTAGGTATCCGGAAGTTTTTGTGAAGGTTATCTGTGGAAATCAGGTATTGAGGACTAGAATATCACAGAGCAAAAGTATTAACCCAATGTGGAATGAGGATTTGATGTTTGTTGCTGCTGAACCGTTTGAGGAGCCGTTGTATCTGACTGTGGAAGATAGAGTGGGATCTGGCAAAGATGACATCTTGGGAAGGTGTGTGATTCCTTTACAGAATGTGCAGAGGAGGTTAGACCATAAACCTGTGCACACTAGGTGGTATAATCTTGAGAAGCATATGATCGTAGACGGGGAACAGAAGAGGGAGGTCAAATTTGCGAGCAGGATTCATTTACGGATTTGTTTGGATGGCGGGTACCATGTCCTGGATGAATCAACACACTACAGTAGTGATCTCAGGCCAACAGCAAGGCAGTTGTGGAAGTCAAGCATTGGGATTTTTGAGGTAGGGGTTATAAGTGCCGTTGGTCTGATGCCAATGAAAACAAAAGATGGCCGAGGTACCACAGATGCTTATTGCGTAGCAAAATACGGGAGGAAATGGGTTCGAACAAGGACAATCGTGGACAGCTTCAATCCTAAGTGGAATGAGCAGTACATCTGGGAGGTTTTCGACCCGTGTACTGTGATTACAATTGGGGTTTTTGACAATGGTCATATACATGGACATGGAAGTGATAAAGGGGGAAAGGATTCAAGAATTGGCAAAGTGAGAATTCGGCTATCTACACTTGAAGCTGACAGGGTTTACACGCATTCGTATCCTCTTCTGGTCCTACAACCATCTGGGGTGAAGAAAATGGGTGAAATTCAGCTGGCAGTGAGGTTTACATGCTCATCTTTGATTAATATGTTGTATATGTATTCGCATCCCCTGTTGCCAAAAATGCACTACATTCATCCATTGTCCGTGCTTCAGCTCGATAGCTTGAGGCACCAGGCTATGCAGATTGTCTCGATGAGGCTGAACCGGTCTGAGCCTCCCCTGAGGAAAGAGGTTGTGGAGTATATGCTGGATGTTGATTCACATATGTGGAGCATGAGAAGAAGCAAGGCCAATTTTTTCCGAATAATGGGAGTTTTAAGCGGGTTGATTGCAGTAGGAAAATGGCTTGATCAAATCTGCAATTGGAAGAATCCTCTCACGACCATTTTAATTCATATCCTCTTTATTATATTGGTTCTTTATCCGGAACTAATTCTTCCCACTATTTTTCTCTACCTATTCTTGATTGGAATTTGGAACTTCCGGCGGAGGCCAAGGCACCCTCCTCACATGGACATCCGACTATCTCATGCTGACGCTGCTCATCCTGACGAACTAGATGAAGAGTTTGACACGTTCCCAACTACCCGGCCATCAGATATAGTGAGGATGAGATATGATCGACTAAGAAGCATAGCAGGGAGGGTTCAAACTGTTGTCGGTGATCTGGCAACTCAAGGCGAAAGGTTTCAGTCTCTTCTGAGTTGGAGAGACCCTAGAGCCACCACCCTGTTCGTAACATTCTGTTTGATTGCCGCCATTGTTCTCTATGTAACTCCATTCCAAGTTGTGGCCCTTCTAGCAGGCATTTACATGTTGAGGCATCCCAAGTTCCGCCAAAAACTTCCTTCAGTGCCACTCAACTTCTTTAGAAGGTTGCCTGCAAGATCAGACAGCATGCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023052 GO:0031224 GO:0031974 GO:0031981 GO:0035556 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 PRT_C

Protein Analysis

775

Amino Acids

88.94

Weight (kDa)

9.17

Isoelectric Point (pI)

44.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	41 - 143	2.4e-22	C2 domain
C2	PF00168	202 - 307	8.2e-23	C2 domain
C2	PF00168	364 - 474	6.8e-21	C2 domain
Pex24p	PF06398	591 - 755	9.9e-07	Integral peroxisomal membrane peroxin
PRT_C	PF08372	619 - 774	6.3e-82	Plant phosphoribosyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005951)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12970
fragaria_vesca	FvH4_3g43560 FvH4_3g43560
malus_domestica	MD03G1021000.v1.1 MD11G1022700.v1.1
prunus_persica	Prupe.6G018400_v2.0.a1 Prupe.6G018400_v2.0.a1 Prupe.6G018400_v2.0.a1
pyrus_communis	pycom03g01800 pycom03g01810 pycom11g01840 pycom11g01850
rosa_chinensis	RchiOBHm_Chr5g0078121
rosa_laevigata	RLG00000036753
rosa_multiflora	Rmu_co8493933.1_g000001 Rmu_sc0000670.1_g000002 Rmu_sc0019972.1_g000003
rosa_roxburghii	Rroxscaffold_1G00000440 Rroxscaffold_1G00004010
rosa_rugosa	Rorug05G0458700 Rorug05G0458700 Rorug05G0458800
rosa_samantha	Rh5AG513300 Rh5BG536400 Rh5CG559800 Rh5DG547900
rosa_wichuraiana	Rw5G047610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1109
Acc65I	GGTACC	2 cut(s)	1008, 1154
AccB1I	GGYRCC	5 cut(s)	415, 1008, 1154, 1587, 1917
AccB7I	CCANNNNNTGG	4 cut(s)	56, 600, 1018, 2209
AccI	GTMKAC	2 cut(s)	582, 939
AccII	CGCG	2 cut(s)	62, 393
AccIII	TCCGGA	2 cut(s)	664, 1843
AciI	CCGC	6 cut(s)	62, 1005, 1740, 1906, 2175, 2254
AclWI	GGATC	3 cut(s)	179, 378, 829
AcoI	YGGCCR	2 cut(s)	1147, 2009
AcsI	RAATTY	4 cut(s)	965, 1368, 1461, 1890
AcuI	CTGAAG	3 cut(s)	285, 1553, 2253
AcvI	CACGTG	1 cut(s)	136
AdeI	CACNNNGTG	2 cut(s)	139, 909
AfaI	GTAC	4 cut(s)	1010, 1156, 1250, 1276
AfiI	CCNNNNNNNGG	5 cut(s)	56, 600, 1018, 1726, 2209
AflIII	ACRYGT	2 cut(s)	1992, 2232
AgeI	ACCGGT	1 cut(s)	1623
AgsI	TTSAA	7 cut(s)	197, 296, 626, 1228, 1350, 1388, 2069
AjnI	CCWGG	2 cut(s)	1017, 1590
AleI	CACNNNNGTG	1 cut(s)	1684
Alw21I	GWGCWC	1 cut(s)	903
Alw26I	GTCTC	5 cut(s)	24, 35, 1612, 2118, 2124
Alw44I	GTGCAC	1 cut(s)	899
AlwI	GGATC	3 cut(s)	179, 378, 829
Aor13HI	TCCGGA	2 cut(s)	664, 1843
AoxI	GGCC	7 cut(s)	548, 1052, 1147, 1707, 1910, 2009, 2211
ApaLI	GTGCAC	1 cut(s)	899
ApeKI	GCWGC	3 cut(s)	536, 770, 1958
ApoI	RAATTY	4 cut(s)	965, 1368, 1461, 1890
AseI	ATTAAT	1 cut(s)	1500
AsiGI	ACCGGT	1 cut(s)	1623
Asp700I	GAANNNNTTC	4 cut(s)	291, 1345, 1851, 2106
Asp718I	GGTACC	2 cut(s)	1008, 1154
AspLEI	GCGC	1 cut(s)	62
AspS9I	GGNCC	3 cut(s)	548, 1426, 2212
AsuC2I	CCSGG	1 cut(s)	2008
AsuHPI	GGTGA	5 cut(s)	151, 331, 1456, 1469, 2093
AsuII	TTCGAA	1 cut(s)	1201
AvaII	GGWCC	1 cut(s)	1426
AxyI	CCTNAGG	2 cut(s)	552, 1640
BaeGI	GKGCMC	1 cut(s)	903
BanI	GGYRCC	5 cut(s)	415, 1008, 1154, 1587, 1917
BanII	GRGCYC	1 cut(s)	68
BbrPI	CACGTG	1 cut(s)	136
BbsI	GAAGAC	1 cut(s)	284
Bbv12I	GWGCWC	1 cut(s)	903
BbvI	GCAGC	3 cut(s)	523, 757, 1945
BccI	CCATC	6 cut(s)	361, 481, 995, 1139, 1444, 2020
BceAI	ACGGC	2 cut(s)	775, 1100
BciT130I	CCWGG	2 cut(s)	1019, 1592
BciVI	GTATCC	2 cut(s)	672, 1425
BclI	TGATCA	1 cut(s)	1768
BcnI	CCSGG	1 cut(s)	2008
BcoDI	GTCTC	5 cut(s)	24, 35, 1612, 2118, 2124
BfaI	CTAG	5 cut(s)	711, 906, 1976, 2136, 2219
BfmI	CTRYAG	1 cut(s)	1036
BfuI	GTATCC	2 cut(s)	672, 1425
BisI	GCNGC	4 cut(s)	537, 771, 1959, 2175
BlsI	GCNGC	4 cut(s)	538, 772, 1960, 2176
Bme1390I	CCNGG	3 cut(s)	1019, 1592, 2008
Bme18I	GGWCC	1 cut(s)	1426
BmgT120I	GGNCC	3 cut(s)	548, 1426, 2212
BmiI	GGNNCC	6 cut(s)	417, 789, 1010, 1156, 1589, 1919
BmrFI	CCNGG	3 cut(s)	1019, 1592, 2008
BmsI	GCATC	8 cut(s)	105, 462, 508, 523, 1116, 1153, 1531, 2251
BoxI	GACNNNNGTC	1 cut(s)	1305
BpiI	GAAGAC	1 cut(s)	284
BpmI	CTGGAG	1 cut(s)	455
Bpu10I	CCTNAGC	1 cut(s)	630
Bpu14I	TTCGAA	1 cut(s)	1201
BpuEI	CTTGAG	5 cut(s)	6, 480, 941, 1603, 2079
BpuMI	CCSGG	1 cut(s)	2008
BsaAI	YACGTR	1 cut(s)	136
BsaI	GGTCTC	2 cut(s)	35, 2124
BsaJI	CCNNGG	2 cut(s)	1150, 1913
BsaWI	WCCGGW	3 cut(s)	664, 1623, 1843
BsaXI	ACNNNNNCTCC	2 cut(s)	779, 809
Bsc4I	CCNNNNNNNGG	5 cut(s)	56, 600, 1018, 1726, 2209
Bse118I	RCCGGY	1 cut(s)	1623
Bse1I	ACTGG	1 cut(s)	178
Bse21I	CCTNAGG	2 cut(s)	552, 1640
BseAI	TCCGGA	2 cut(s)	664, 1843
BseBI	CCWGG	2 cut(s)	1019, 1592
BseDI	CCNNGG	2 cut(s)	1150, 1913
BseLI	CCNNNNNNNGG	5 cut(s)	56, 600, 1018, 1726, 2209
BseMII	CTCAG	6 cut(s)	543, 644, 1063, 1620, 1631, 2111
BseNI	ACTGG	1 cut(s)	178
BseRI	GAGGAG	3 cut(s)	800, 892, 1914
BseSI	GKGCMC	1 cut(s)	903
BseXI	GCAGC	3 cut(s)	523, 757, 1945
BsgI	GTGCAG	1 cut(s)	893
Bsh1236I	CGCG	2 cut(s)	62, 393
Bsh1285I	CGRYCG	1 cut(s)	575
BshFI	GGCC	7 cut(s)	550, 1054, 1149, 1709, 1912, 2011, 2213
BshNI	GGYRCC	5 cut(s)	415, 1008, 1154, 1587, 1917
BshTI	ACCGGT	1 cut(s)	1623
BsiEI	CGRYCG	1 cut(s)	575
BsiHKAI	GWGCWC	1 cut(s)	903
BsiSI	CCGG	5 cut(s)	665, 1624, 1844, 1903, 2008
BslI	CCNNNNNNNGG	5 cut(s)	56, 600, 1018, 1726, 2209
BsmAI	GTCTC	5 cut(s)	24, 35, 1612, 2118, 2124
BsmBI	CGTCTC	1 cut(s)	24
BsmI	GAATGC	2 cut(s)	526, 1408
BsnI	GGCC	7 cut(s)	550, 1054, 1149, 1709, 1912, 2011, 2213
Bso31I	GGTCTC	2 cut(s)	35, 2124
Bsp119I	TTCGAA	1 cut(s)	1201
Bsp1286I	GDGCHC	2 cut(s)	68, 903
Bsp13I	TCCGGA	2 cut(s)	664, 1843
BspACI	CCGC	6 cut(s)	62, 1005, 1740, 1906, 2175, 2254
BspANI	GGCC	7 cut(s)	550, 1054, 1149, 1709, 1912, 2011, 2213
BspCNI	CTCAG	6 cut(s)	544, 643, 1062, 1621, 1632, 2112
BspEI	TCCGGA	2 cut(s)	664, 1843
BspFNI	CGCG	2 cut(s)	62, 393
BspLI	GGNNCC	6 cut(s)	417, 789, 1010, 1156, 1589, 1919
BspPI	GGATC	3 cut(s)	179, 378, 829
BspT104I	TTCGAA	1 cut(s)	1201
BspT107I	GGYRCC	5 cut(s)	415, 1008, 1154, 1587, 1917
BspTNI	GGTCTC	2 cut(s)	35, 2124
BsrFI	RCCGGY	1 cut(s)	1623
BsrI	ACTGG	1 cut(s)	178
BssAI	RCCGGY	1 cut(s)	1623
BssECI	CCNNGG	2 cut(s)	1150, 1913
BssNAI	GTATAC	1 cut(s)	583
BssT1I	CCWWGG	1 cut(s)	1913
Bst1107I	GTATAC	1 cut(s)	583
Bst2UI	CCWGG	2 cut(s)	1019, 1592
Bst4CI	ACNGT	7 cut(s)	18, 544, 780, 805, 1040, 1279, 2074
Bst6I	CTCTTC	4 cut(s)	947, 1425, 1977, 2121
BstBAI	YACGTR	1 cut(s)	136
BstBI	TTCGAA	1 cut(s)	1201
BstC8I	GCNNGC	8 cut(s)	64, 159, 477, 973, 1470, 2224, 2301, 2318
BstDEI	CTNAG	9 cut(s)	228, 552, 630, 1049, 1233, 1629, 1640, 2045, 2120
BstFNI	CGCG	2 cut(s)	62, 393
BstHHI	GCGC	1 cut(s)	62
BstMAI	GTCTC	5 cut(s)	24, 35, 1612, 2118, 2124
BstMCI	CGRYCG	1 cut(s)	575
BstMWI	GCNNNNNNNGC	4 cut(s)	506, 1540, 1958, 2219
BstNI	CCWGG	2 cut(s)	1019, 1592
BstNSI	RCATGY	3 cut(s)	1488, 2236, 2320
BstPAI	GACNNNNGTC	1 cut(s)	1305
BstSCI	CCNGG	3 cut(s)	1017, 1590, 2006
BstSFI	CTRYAG	1 cut(s)	1036
BstSLI	GKGCMC	1 cut(s)	903
BstUI	CGCG	2 cut(s)	62, 393
BstV1I	GCAGC	3 cut(s)	523, 757, 1945
BstV2I	GAAGAC	1 cut(s)	284
BstX2I	RGATCY	2 cut(s)	370, 821
BstYI	RGATCY	2 cut(s)	370, 821
BstZ17I	GTATAC	1 cut(s)	583
Bsu36I	CCTNAGG	2 cut(s)	552, 1640
BsuI	GTATCC	2 cut(s)	672, 1425
BsuRI	GGCC	7 cut(s)	550, 1054, 1149, 1709, 1912, 2011, 2213
BtsI	GCAGTG	1 cut(s)	1479
BtsIMutI	CAGTG	3 cut(s)	171, 1479, 2277
Cac8I	GCNNGC	8 cut(s)	64, 159, 477, 973, 1470, 2224, 2301, 2318
CfoI	GCGC	1 cut(s)	62
Cfr10I	RCCGGY	1 cut(s)	1623
Cfr13I	GGNCC	3 cut(s)	548, 1426, 2212
CseI	GACGC	1 cut(s)	1964
Csp6I	GTAC	4 cut(s)	1009, 1155, 1249, 1275
CspAI	ACCGGT	1 cut(s)	1623
CviQI	GTAC	4 cut(s)	1009, 1155, 1249, 1275
DdeI	CTNAG	9 cut(s)	228, 552, 630, 1049, 1233, 1629, 1640, 2045, 2120
DraIII	CACNNNGTG	2 cut(s)	139, 909
EaeI	YGGCCR	2 cut(s)	1147, 2009
Eam1104I	CTCTTC	4 cut(s)	947, 1425, 1977, 2121
EarI	CTCTTC	4 cut(s)	947, 1425, 1977, 2121
EciI	GGCGGA	2 cut(s)	1921, 2243
Eco130I	CCWWGG	1 cut(s)	1913
Eco24I	GRGCYC	1 cut(s)	68
Eco31I	GGTCTC	2 cut(s)	35, 2124
Eco32I	GATATC	1 cut(s)	382
Eco47I	GGWCC	1 cut(s)	1426
Eco57I	CTGAAG	3 cut(s)	285, 1553, 2253
Eco72I	CACGTG	1 cut(s)	136
Eco81I	CCTNAGG	2 cut(s)	552, 1640
EcoRI	GAATTC	1 cut(s)	1368
EcoRII	CCWGG	2 cut(s)	1017, 1590
EcoRV	GATATC	1 cut(s)	382
EcoT14I	CCWWGG	1 cut(s)	1913
EcoT22I	ATGCAT	1 cut(s)	523
EcoT38I	GRGCYC	1 cut(s)	68
ErhI	CCWWGG	1 cut(s)	1913
Esp3I	CGTCTC	1 cut(s)	24
FalI	AAGNNNNNCTT	2 cut(s)	276, 308
FauI	CCCGC	3 cut(s)	55, 998, 1733
FauNDI	CATATG	2 cut(s)	930, 1683
FbaI	TGATCA	1 cut(s)	1768
FblI	GTMKAC	2 cut(s)	582, 939
Fnu4HI	GCNGC	4 cut(s)	537, 771, 1959, 2175
FriOI	GRGCYC	1 cut(s)	68
Fsp4HI	GCNGC	4 cut(s)	537, 771, 1959, 2175
FspBI	CTAG	5 cut(s)	711, 906, 1976, 2136, 2219
GlaI	GCGC	1 cut(s)	61
GluI	GCNGC	4 cut(s)	537, 771, 1959, 2175
GsuI	CTGGAG	1 cut(s)	455
HaeIII	GGCC	7 cut(s)	550, 1054, 1149, 1709, 1912, 2011, 2213
HapII	CCGG	5 cut(s)	665, 1624, 1844, 1903, 2008
HgaI	GACGC	1 cut(s)	1964
HhaI	GCGC	1 cut(s)	62
Hin6I	GCGC	1 cut(s)	60
HinP1I	GCGC	1 cut(s)	60
HindIII	AAGCTT	2 cut(s)	297, 596
HinfI	GANTC	7 cut(s)	262, 856, 979, 1025, 1346, 1676, 1789
HpaII	CCGG	5 cut(s)	665, 1624, 1844, 1903, 2008
HphI	GGTGA	5 cut(s)	151, 331, 1456, 1469, 2093
Hpy166II	GTNNAC	8 cut(s)	133, 583, 901, 940, 1219, 1275, 1402, 1482
Hpy8I	GTNNAC	8 cut(s)	133, 583, 901, 940, 1219, 1275, 1402, 1482
HpyAV	CCTTC	7 cut(s)	439, 571, 673, 840, 2225, 2277, 2286
HpyCH4III	ACNGT	7 cut(s)	18, 544, 780, 805, 1040, 1279, 2074
HpyCH4IV	ACGT	4 cut(s)	129, 135, 289, 1994
HpyF10VI	GCNNNNNNNGC	4 cut(s)	506, 1540, 1958, 2219
HpyF3I	CTNAG	9 cut(s)	228, 552, 630, 1049, 1233, 1629, 1640, 2045, 2120
HpySE526I	ACGT	4 cut(s)	129, 135, 289, 1994
HspAI	GCGC	1 cut(s)	60
Kpn2I	TCCGGA	2 cut(s)	664, 1843
KpnI	GGTACC	2 cut(s)	1012, 1158
Ksp22I	TGATCA	1 cut(s)	1768
LmnI	GCTCC	2 cut(s)	787, 1689
Lsp1109I	GCAGC	3 cut(s)	523, 757, 1945
LweI	GCATC	8 cut(s)	105, 462, 508, 523, 1116, 1153, 1531, 2251
MaeI	CTAG	5 cut(s)	711, 906, 1976, 2136, 2219
MaeII	ACGT	4 cut(s)	129, 135, 289, 1994
MaeIII	GTNAC	4 cut(s)	169, 223, 2155, 2191
MfeI	CAATTG	2 cut(s)	1287, 1780
MflI	RGATCY	2 cut(s)	370, 821
MhlI	GDGCHC	2 cut(s)	68, 903
MmeI	TCCRAC	2 cut(s)	1963, 2105
Mph1103I	ATGCAT	1 cut(s)	523
MroI	TCCGGA	2 cut(s)	664, 1843
MroXI	GAANNNNTTC	4 cut(s)	291, 1345, 1851, 2106
MseI	TTAA	6 cut(s)	561, 615, 735, 1500, 1736, 1808
MslI	CAYNNNNRTG	7 cut(s)	421, 531, 1314, 1320, 1326, 1684, 2231
MspA1I	CMGCKG	1 cut(s)	1468
MspI	CCGG	5 cut(s)	665, 1624, 1844, 1903, 2008
MspR9I	CCNGG	3 cut(s)	1019, 1592, 2008
MunI	CAATTG	2 cut(s)	1287, 1780
Mva1269I	GAATGC	2 cut(s)	526, 1408
MvaI	CCWGG	2 cut(s)	1019, 1592
MvnI	CGCG	2 cut(s)	62, 393
MwoI	GCNNNNNNNGC	4 cut(s)	506, 1540, 1958, 2219
NciI	CCSGG	1 cut(s)	2008
NdeI	CATATG	2 cut(s)	930, 1683
NlaIV	GGNNCC	6 cut(s)	417, 789, 1010, 1156, 1589, 1919
NmeAIII	GCCGAG	1 cut(s)	1175
NmuCI	GTSAC	1 cut(s)	169
NsiI	ATGCAT	1 cut(s)	523
NspI	RCATGY	3 cut(s)	1488, 2236, 2320
NspV	TTCGAA	1 cut(s)	1201
OliI	CACNNNNGTG	1 cut(s)	1684
PaeI	GCATGC	1 cut(s)	2320
PciI	ACATGT	1 cut(s)	2232
PctI	GAATGC	2 cut(s)	526, 1408
PdmI	GAANNNNTTC	4 cut(s)	291, 1345, 1851, 2106
PfeI	GAWTC	7 cut(s)	262, 856, 979, 1025, 1346, 1676, 1789
PflMI	CCANNNNNTGG	4 cut(s)	56, 600, 1018, 2209
PfoI	TCCNGGA	1 cut(s)	1017
PinAI	ACCGGT	1 cut(s)	1623
PkrI	GCNGC	4 cut(s)	538, 772, 1960, 2176
Ple19I	CGATCG	1 cut(s)	575
PmaCI	CACGTG	1 cut(s)	136
PmlI	CACGTG	1 cut(s)	136
Ppu21I	YACGTR	1 cut(s)	136
PscI	ACATGT	1 cut(s)	2232
PshAI	GACNNNNGTC	1 cut(s)	1305
PshBI	ATTAAT	1 cut(s)	1500
PsiI	TTATAA	1 cut(s)	1109
Psp6I	CCWGG	2 cut(s)	1017, 1590
PspCI	CACGTG	1 cut(s)	136
PspGI	CCWGG	2 cut(s)	1017, 1590
PspN4I	GGNNCC	6 cut(s)	417, 789, 1010, 1156, 1589, 1919
PspPI	GGNCC	3 cut(s)	548, 1426, 2212
PsuI	RGATCY	2 cut(s)	370, 821
PvuI	CGATCG	1 cut(s)	575
PvuII	CAGCTG	1 cut(s)	1468
RsaI	GTAC	4 cut(s)	1010, 1156, 1250, 1276
RsaNI	GTAC	4 cut(s)	1009, 1155, 1249, 1275
RseI	CAYNNNNRTG	7 cut(s)	421, 531, 1314, 1320, 1326, 1684, 2231
SaqAI	TTAA	6 cut(s)	561, 615, 735, 1500, 1736, 1808
SatI	GCNGC	4 cut(s)	537, 771, 1959, 2175
Sau96I	GGNCC	3 cut(s)	548, 1426, 2212
ScrFI	CCNGG	3 cut(s)	1019, 1592, 2008
SduI	GDGCHC	2 cut(s)	68, 903
SfaNI	GCATC	8 cut(s)	105, 462, 508, 523, 1116, 1153, 1531, 2251
SfcI	CTRYAG	1 cut(s)	1036
SfuI	TTCGAA	1 cut(s)	1201
SinI	GGWCC	1 cut(s)	1426
SmiMI	CAYNNNNRTG	7 cut(s)	421, 531, 1314, 1320, 1326, 1684, 2231
SmlI	CTYRAG	5 cut(s)	21, 495, 920, 1582, 2094
SmoI	CTYRAG	5 cut(s)	21, 495, 920, 1582, 2094
SphI	GCATGC	1 cut(s)	2320
SsiI	CCGC	6 cut(s)	62, 1005, 1740, 1906, 2175, 2254
SspMI	CTAG	5 cut(s)	711, 906, 1976, 2136, 2219
StyD4I	CCNGG	3 cut(s)	1017, 1590, 2006
StyI	CCWWGG	1 cut(s)	1913
TaaI	ACNGT	7 cut(s)	18, 544, 780, 805, 1040, 1279, 2074
TaiI	ACGT	4 cut(s)	132, 138, 292, 1997
TaqI	TCGA	8 cut(s)	237, 571, 575, 1201, 1266, 1575, 1610, 2041
TatI	WGTACW	2 cut(s)	1248, 1274
TauI	GCSGC	1 cut(s)	2177
TfiI	GAWTC	7 cut(s)	262, 856, 979, 1025, 1346, 1676, 1789
Tru1I	TTAA	6 cut(s)	561, 615, 735, 1500, 1736, 1808
Tru9I	TTAA	6 cut(s)	561, 615, 735, 1500, 1736, 1808
TscAI	CASTG	3 cut(s)	178, 1479, 2277
TseFI	GTSAC	1 cut(s)	169
TseI	GCWGC	3 cut(s)	536, 770, 1958
Tsp45I	GTSAC	1 cut(s)	169
TspDTI	ATGAA	7 cut(s)	340, 971, 1038, 1148, 1541, 1802, 1995
TspGWI	ACGGA	3 cut(s)	127, 1003, 1552
TspRI	CASTG	3 cut(s)	178, 1479, 2277
Van91I	CCANNNNNTGG	4 cut(s)	56, 600, 1018, 2209
VneI	GTGCAC	1 cut(s)	899
VpaK11BI	GGWCC	1 cut(s)	1426
VspI	ATTAAT	1 cut(s)	1500
XapI	RAATTY	4 cut(s)	965, 1368, 1461, 1890
XceI	RCATGY	3 cut(s)	1488, 2236, 2320
XmiI	GTMKAC	2 cut(s)	582, 939
XmnI	GAANNNNTTC	4 cut(s)	291, 1345, 1851, 2106
XspI	CTAG	5 cut(s)	711, 906, 1976, 2136, 2219
Zsp2I	ATGCAT	1 cut(s)	523

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.