Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005951

Physical Location & Seq

Genomic Coordinates Reverse (-)

1983736 .. 1987052

Gene Structure 3317 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1022700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGCAGAAGCCTCCGCAGTCTCAAGATTTTGCTTTGAAAGAGACGTCGCCAAACATTGGCGCGGGCTCTATCACGGGGGACAAGCTCTCATGCACATATGACCTCGTAGAACAGATGCACTACCTTTACGTTCGTGTGGTGAAAGCAAAGGATTTGCCTGCAAAAGATGTCACTGGTAGTTGTGATCCCTATGTCGAAGTGAAACTTGGAAACTATAAGGGAGTTACTAGGCATTTTGAGAAGAAGTCCAACCCTGAATGGAATCAGGTGTTTGCTTTCTCCAAAGATCGACTTCAAGCTTCATTTTTGGAAGTTGTGGTGAAAGATAAGGATGTTGTGCTAGATGATTTCATGGGCAGGATTATGTTTGAGCTCAATGACATCCCGAAACGCTTTCCACCAGATAGCCCACTGGCACCACAGTGGTATAGGTTGGAGGACAGGAAGGGGGTTAAGGTCAAGGGGGAGCTGATGTTGGCAGTTTGGATGGGAACTCAAGCAGACGAGGCGTTTCCTGATGCGTGGCATTCTGATGCTGCAACAGTTGGGCCCGAGGGTGTCAATAACATTCGATCGAAGGTATACCTCTCCCCCAAGCTTTGGTATGTGAGGGTTAATGTTATTGAAGCTCAGGACTTGCTACCTAATGACAAGAGTAGGTATCCGGAAGTTTTTGTGAAGGTTATCTGTGGAAATCAGGTATTGAGGACTAGAATATCACAGAGCAAAAGTATTAACCCAATTTGGAATGAGGATTTGATGTTTGTTGCTGCTGAACCATTTGAGGAACCGTTGTATCTTACTGTGGAAGATAGAGTGGGATCCGGCAAAGATGAAATTTTGGGTAAGTGTGTGATTGCTTTACAGACTGTGCAGAGGAGGTTAGACCATAAACCTGTGCACACGAGGTGGTTTAATCTTGAGAAGCATATGATCGTAGATGGGGAACAGAAGAGGGAGATCAAATTTTCCAGCAGGATTCATTTACGGATTTGCTTGGATGGTGGGTACCATGTCTTGGATGAATCAACACACTACAGTAGTGATCTCAGGCCAACTGCAAAGCAGTTGTGGAAGTCGAGCATCGGGATTTTTGAGGTAGGGGTTATAAGCGCAGTTGGTTTGATGCCAATGAAAACAAAAGATGGCCGAGGTACCACAGATGCTTATTGTGTAGCAAAATACGGGCAGAAATGGGTTCGCACAAGGACAATCGTGGACAGCTTCAATCCCAAGTGGAATGAGCAGTACATCTGGGAGGTTTTCGACCCGTGTACTGTCATTACAATTGGGGTTTTTGACAATGGTCATATACATGGAGGTGATAAAGGGGGAAAGGATTCAAGAATTGGCAAAGTGAGAATTCGGCTATCTACACTTGAAGCTGACAGGGTTTACACACATTCGTACCCTCTTCTGGTCCTACATCCATCTGGGGTGAAGAAAATGGGTGAAATTCAGCTGGCAGTGAGGTTTACATGCTCATCTTTGATTAATATGCTGTATATGTATTCGCATCCTTTGTTGCCAAAAATGCATTACATTCATCCATTGTCTGTGATTCAGCTCGATAGCTTGAGGCACCAGGCTATGCAGATTGTCTCAATGAGGCTGAACCGGGCTGAGCCCCCACTGAGGAAAGAGGTTGTGGAGTATATGCTGGATGTTGATTCACATATGTGGAGCATGAGAAGAAGCAAGGCAAATTTTTTCCGAATAATGGGAGTTTTAAGCGGGGTGATTGCAGTAGGAAAATGGCTTGATCAAATCTGCAATTGGAAGAATCCTCTCACAACCATTTTAATTCATATCCTCTTTATTATATTGGTTCTTTATCCGGAACTAATTCTTCCAACTATTTTTCTCTACCTATTCTTGATTGGAATTTGGAACTACCGGTGGAGGCCGAGGCACCCTCCTCACATGGACACACGGCTATCTCATGCTGATGCTGCTCATCCTGACGAACGAGATGAAGAGTTTGACACATTCCCAACTTCTCGGCCATCAGATATTGTGAGAATGAGATATGATCGACTAAGAAGCATAGCAGGGAGGGTTCAGACTGTTGTCGGTGATCTGGCAACTCAAGGGGAAAGGTTTCAGTCTCTTCTGAGTTGGAGAGACCCTAGAGCCACAACCCTGTTCGTAACTTTCTGTTTGATTGCCGCCATTGTTCTCTATGTAACTCCATTCCAAGTTGTGGCCCTTCTAGCAGGCATTTACGTGTTAAGGCATCCCAGATTACGCCACAAACTTCCTTCAGTGCCACTCAACTTCTTTAGGAGGTTGCCTGCAAGATCAGACAGCATGCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023052 GO:0031224 GO:0031974 GO:0031981 GO:0035556 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 PRT_C

Protein Analysis

773

Amino Acids

88.75

Weight (kDa)

9.15

Isoelectric Point (pI)

45.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	41 - 145	4.3e-22	C2 domain
C2	PF00168	202 - 307	9e-22	C2 domain
C2	PF00168	364 - 472	1.7e-21	C2 domain
Pex24p	PF06398	589 - 752	5.2e-07	Integral peroxisomal membrane peroxin
PRT_C	PF08372	617 - 772	5.8e-81	Plant phosphoribosyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005951)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12970
fragaria_vesca	FvH4_3g43560 FvH4_3g43560
malus_domestica	MD03G1021000.v1.1 MD11G1022700.v1.1
prunus_persica	Prupe.6G018400_v2.0.a1 Prupe.6G018400_v2.0.a1 Prupe.6G018400_v2.0.a1
pyrus_communis	pycom03g01800 pycom03g01810 pycom11g01840 pycom11g01850
rosa_chinensis	RchiOBHm_Chr5g0078121
rosa_laevigata	RLG00000036753
rosa_multiflora	Rmu_co8493933.1_g000001 Rmu_sc0000670.1_g000002 Rmu_sc0019972.1_g000003
rosa_roxburghii	Rroxscaffold_1G00000440 Rroxscaffold_1G00004010
rosa_rugosa	Rorug05G0458700 Rorug05G0458700 Rorug05G0458800
rosa_samantha	Rh5AG513300 Rh5BG536400 Rh5CG559800 Rh5DG547900
rosa_wichuraiana	Rw5G047610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1109
AatII	GACGTC	1 cut(s)	47
Acc65I	GGTACC	2 cut(s)	1008, 1154
AccB1I	GGYRCC	5 cut(s)	415, 1008, 1154, 1581, 1911
AccB7I	CCANNNNNTGG	4 cut(s)	56, 600, 1018, 2203
AccI	GTMKAC	1 cut(s)	582
AccII	CGCG	1 cut(s)	62
AccIII	TCCGGA	2 cut(s)	664, 1837
AciI	CCGC	4 cut(s)	14, 62, 1734, 2169
AclWI	GGATC	3 cut(s)	179, 816, 829
AcoI	YGGCCR	2 cut(s)	1147, 2003
AcsI	RAATTY	6 cut(s)	837, 965, 1362, 1455, 1705, 1884
AcuI	CTGAAG	1 cut(s)	2247
AcyI	GRCGYC	1 cut(s)	44
AdeI	CACNNNGTG	1 cut(s)	909
AfaI	GTAC	5 cut(s)	1010, 1156, 1250, 1276, 1409
AfiI	CCNNNNNNNGG	7 cut(s)	56, 600, 1018, 1417, 1635, 1720, 2203
AflIII	ACRYGT	1 cut(s)	2226
AgeI	ACCGGT	1 cut(s)	1896
AgsI	TTSAA	6 cut(s)	37, 296, 626, 1228, 1344, 1382
AjnI	CCWGG	1 cut(s)	1584
AjuI	GAANNNNNNNTTGG	2 cut(s)	189, 221
AleI	CACNNNNGTG	2 cut(s)	421, 1678
Alw21I	GWGCWC	2 cut(s)	375, 903
Alw26I	GTCTC	5 cut(s)	24, 35, 1606, 2112, 2118
Alw44I	GTGCAC	1 cut(s)	899
AlwI	GGATC	3 cut(s)	179, 816, 829
Ama87I	CYCGRG	1 cut(s)	551
Aor13HI	TCCGGA	2 cut(s)	664, 1837
AoxI	GGCC	6 cut(s)	548, 1052, 1147, 1904, 2003, 2205
ApaI	GGGCCC	1 cut(s)	552
ApaLI	GTGCAC	1 cut(s)	899
ApeKI	GCWGC	3 cut(s)	536, 770, 1952
ApoI	RAATTY	6 cut(s)	837, 965, 1362, 1455, 1705, 1884
AseI	ATTAAT	1 cut(s)	1494
AsiGI	ACCGGT	1 cut(s)	1896
Asp700I	GAANNNNTTC	3 cut(s)	1339, 1845, 2100
Asp718I	GGTACC	2 cut(s)	1008, 1154
AspLEI	GCGC	2 cut(s)	62, 1115
AspS9I	GGNCC	4 cut(s)	548, 549, 1420, 2206
AsuC2I	CCSGG	1 cut(s)	1619
AsuHPI	GGTGA	7 cut(s)	151, 331, 1334, 1450, 1463, 1750, 2087
AvaI	CYCGRG	1 cut(s)	551
AvaII	GGWCC	1 cut(s)	1420
BaeGI	GKGCMC	2 cut(s)	552, 903
BaeI	ACNNNNGTAYC	4 cut(s)	779, 812, 1391, 1424
BamHI	GGATCC	1 cut(s)	821
BanI	GGYRCC	5 cut(s)	415, 1008, 1154, 1581, 1911
BanII	GRGCYC	4 cut(s)	68, 375, 552, 1629
BauI	CACGAG	1 cut(s)	904
Bbv12I	GWGCWC	2 cut(s)	375, 903
BbvI	GCAGC	3 cut(s)	523, 757, 1939
BccI	CCATC	6 cut(s)	481, 935, 995, 1139, 1438, 2014
BceAI	ACGGC	1 cut(s)	1949
BciT130I	CCWGG	1 cut(s)	1586
BciVI	GTATCC	1 cut(s)	672
BclI	TGATCA	1 cut(s)	1762
BcnI	CCSGG	1 cut(s)	1619
BcoDI	GTCTC	5 cut(s)	24, 35, 1606, 2112, 2118
BfaI	CTAG	5 cut(s)	228, 341, 711, 2130, 2213
BfmI	CTRYAG	1 cut(s)	1036
BfuI	GTATCC	1 cut(s)	672
BisI	GCNGC	4 cut(s)	537, 771, 1953, 2169
BlpI	GCTNAGC	1 cut(s)	1623
BlsI	GCNGC	4 cut(s)	538, 772, 1954, 2170
Bme1390I	CCNGG	2 cut(s)	1586, 1619
Bme18I	GGWCC	1 cut(s)	1420
BmeT110I	CYCGRG	1 cut(s)	551
BmgT120I	GGNCC	4 cut(s)	548, 549, 1420, 2206
BmiI	GGNNCC	8 cut(s)	417, 550, 789, 823, 1010, 1156, 1583, 1913
BmrFI	CCNGG	2 cut(s)	1586, 1619
BmsI	GCATC	9 cut(s)	105, 508, 523, 1092, 1116, 1153, 1525, 1939, 2245
BoxI	GACNNNNGTC	2 cut(s)	1305, 2069
BplI	GAGNNNNNCTC	2 cut(s)	1900, 1932
Bpu10I	CCTNAGC	1 cut(s)	630
Bpu1102I	GCTNAGC	1 cut(s)	1623
BpuEI	CTTGAG	5 cut(s)	6, 480, 941, 1597, 2073
BpuMI	CCSGG	1 cut(s)	1619
BsaAI	YACGTR	1 cut(s)	2227
BsaHI	GRCGYC	1 cut(s)	44
BsaI	GGTCTC	1 cut(s)	2118
BsaJI	CCNNGG	3 cut(s)	552, 1150, 1907
BsaWI	WCCGGW	3 cut(s)	664, 1837, 1896
BsaXI	ACNNNNNCTCC	2 cut(s)	458, 488
Bsc4I	CCNNNNNNNGG	7 cut(s)	56, 600, 1018, 1417, 1635, 1720, 2203
Bse118I	RCCGGY	1 cut(s)	1896
Bse1I	ACTGG	2 cut(s)	178, 417
BseAI	TCCGGA	2 cut(s)	664, 1837
BseBI	CCWGG	1 cut(s)	1586
BseDI	CCNNGG	3 cut(s)	552, 1150, 1907
BseLI	CCNNNNNNNGG	7 cut(s)	56, 600, 1018, 1417, 1635, 1720, 2203
BseMII	CTCAG	5 cut(s)	644, 1063, 1614, 1625, 2105
BseNI	ACTGG	2 cut(s)	178, 417
BseRI	GAGGAG	2 cut(s)	892, 1908
BseSI	GKGCMC	2 cut(s)	552, 903
BseXI	GCAGC	3 cut(s)	523, 757, 1939
BsgI	GTGCAG	1 cut(s)	893
Bsh1236I	CGCG	1 cut(s)	62
Bsh1285I	CGRYCG	1 cut(s)	575
BshFI	GGCC	6 cut(s)	550, 1054, 1149, 1906, 2005, 2207
BshNI	GGYRCC	5 cut(s)	415, 1008, 1154, 1581, 1911
BshTI	ACCGGT	1 cut(s)	1896
BsiEI	CGRYCG	1 cut(s)	575
BsiHKAI	GWGCWC	2 cut(s)	375, 903
BsiHKCI	CYCGRG	1 cut(s)	551
BsiSI	CCGG	5 cut(s)	665, 825, 1618, 1838, 1897
BslFI	GGGAC	1 cut(s)	92
BslI	CCNNNNNNNGG	7 cut(s)	56, 600, 1018, 1417, 1635, 1720, 2203
BsmAI	GTCTC	5 cut(s)	24, 35, 1606, 2112, 2118
BsmBI	CGTCTC	1 cut(s)	35
BsmFI	GGGAC	1 cut(s)	92
BsmI	GAATGC	1 cut(s)	526
BsnI	GGCC	6 cut(s)	550, 1054, 1149, 1906, 2005, 2207
Bso31I	GGTCTC	1 cut(s)	2118
BsoBI	CYCGRG	1 cut(s)	551
Bsp120I	GGGCCC	1 cut(s)	548
Bsp1286I	GDGCHC	5 cut(s)	68, 375, 552, 903, 1629
Bsp13I	TCCGGA	2 cut(s)	664, 1837
Bsp1720I	GCTNAGC	1 cut(s)	1623
BspACI	CCGC	4 cut(s)	14, 62, 1734, 2169
BspANI	GGCC	6 cut(s)	550, 1054, 1149, 1906, 2005, 2207
BspCNI	CTCAG	5 cut(s)	643, 1062, 1615, 1626, 2106
BspEI	TCCGGA	2 cut(s)	664, 1837
BspFNI	CGCG	1 cut(s)	62
BspLI	GGNNCC	8 cut(s)	417, 550, 789, 823, 1010, 1156, 1583, 1913
BspPI	GGATC	3 cut(s)	179, 816, 829
BspT107I	GGYRCC	5 cut(s)	415, 1008, 1154, 1581, 1911
BspTNI	GGTCTC	1 cut(s)	2118
BsrFI	RCCGGY	1 cut(s)	1896
BsrI	ACTGG	2 cut(s)	178, 417
BssAI	RCCGGY	1 cut(s)	1896
BssECI	CCNNGG	3 cut(s)	552, 1150, 1907
BssNAI	GTATAC	1 cut(s)	583
BssNI	GRCGYC	1 cut(s)	44
BssSI	CACGAG	1 cut(s)	904
Bst1107I	GTATAC	1 cut(s)	583
Bst2BI	CACGAG	1 cut(s)	904
Bst2UI	CCWGG	1 cut(s)	1586
Bst4CI	ACNGT	8 cut(s)	423, 544, 792, 805, 871, 1040, 1279, 2068
Bst6I	CTCTTC	4 cut(s)	947, 1419, 1971, 2115
BstACI	GRCGYC	1 cut(s)	44
BstBAI	YACGTR	1 cut(s)	2227
BstC8I	GCNNGC	6 cut(s)	64, 159, 1464, 2218, 2295, 2312
BstDEI	CTNAG	6 cut(s)	630, 1049, 1623, 1634, 2039, 2114
BstFNI	CGCG	1 cut(s)	62
BstHHI	GCGC	2 cut(s)	62, 1115
BstMAI	GTCTC	5 cut(s)	24, 35, 1606, 2112, 2118
BstMCI	CGRYCG	1 cut(s)	575
BstMWI	GCNNNNNNNGC	4 cut(s)	506, 1534, 1952, 2213
BstNI	CCWGG	1 cut(s)	1586
BstNSI	RCATGY	2 cut(s)	1482, 2314
BstPAI	GACNNNNGTC	2 cut(s)	1305, 2069
BstSCI	CCNGG	2 cut(s)	1584, 1617
BstSFI	CTRYAG	1 cut(s)	1036
BstSLI	GKGCMC	2 cut(s)	552, 903
BstUI	CGCG	1 cut(s)	62
BstV1I	GCAGC	3 cut(s)	523, 757, 1939
BstX2I	RGATCY	1 cut(s)	821
BstYI	RGATCY	1 cut(s)	821
BstZ17I	GTATAC	1 cut(s)	583
BsuI	GTATCC	1 cut(s)	672
BsuRI	GGCC	6 cut(s)	550, 1054, 1149, 1906, 2005, 2207
BtsI	GCAGTG	1 cut(s)	1473
BtsIMutI	CAGTG	6 cut(s)	171, 410, 428, 1473, 1631, 2271
Cac8I	GCNNGC	6 cut(s)	64, 159, 1464, 2218, 2295, 2312
CfoI	GCGC	2 cut(s)	62, 1115
Cfr10I	RCCGGY	1 cut(s)	1896
Cfr13I	GGNCC	4 cut(s)	548, 549, 1420, 2206
Csp6I	GTAC	5 cut(s)	1009, 1155, 1249, 1275, 1408
CspAI	ACCGGT	1 cut(s)	1896
CviAII	CATG	9 cut(s)	90, 352, 1013, 1316, 1479, 1687, 1924, 1943, 2311
CviQI	GTAC	5 cut(s)	1009, 1155, 1249, 1275, 1408
DdeI	CTNAG	6 cut(s)	630, 1049, 1623, 1634, 2039, 2114
DraIII	CACNNNGTG	1 cut(s)	909
EaeI	YGGCCR	2 cut(s)	1147, 2003
Eam1104I	CTCTTC	4 cut(s)	947, 1419, 1971, 2115
EarI	CTCTTC	4 cut(s)	947, 1419, 1971, 2115
Ecl136II	GAGCTC	1 cut(s)	373
Eco24I	GRGCYC	4 cut(s)	68, 375, 552, 1629
Eco31I	GGTCTC	1 cut(s)	2118
Eco47I	GGWCC	1 cut(s)	1420
Eco53kI	GAGCTC	1 cut(s)	373
Eco57I	CTGAAG	1 cut(s)	2247
Eco88I	CYCGRG	1 cut(s)	551
EcoICRI	GAGCTC	1 cut(s)	373
EcoRI	GAATTC	1 cut(s)	1362
EcoRII	CCWGG	1 cut(s)	1584
EcoT22I	ATGCAT	1 cut(s)	1539
EcoT38I	GRGCYC	4 cut(s)	68, 375, 552, 1629
Esp3I	CGTCTC	1 cut(s)	35
FaeI	CATG	9 cut(s)	93, 355, 1016, 1319, 1482, 1690, 1927, 1946, 2314
FalI	AAGNNNNNCTT	4 cut(s)	189, 221, 276, 308
FaqI	GGGAC	1 cut(s)	92
FatI	CATG	9 cut(s)	89, 351, 1012, 1315, 1478, 1686, 1923, 1942, 2310
FauI	CCCGC	2 cut(s)	55, 1727
FauNDI	CATATG	3 cut(s)	97, 930, 1677
FbaI	TGATCA	1 cut(s)	1762
FblI	GTMKAC	1 cut(s)	582
Fnu4HI	GCNGC	4 cut(s)	537, 771, 1953, 2169
FriOI	GRGCYC	4 cut(s)	68, 375, 552, 1629
Fsp4HI	GCNGC	4 cut(s)	537, 771, 1953, 2169
FspBI	CTAG	5 cut(s)	228, 341, 711, 2130, 2213
GlaI	GCGC	2 cut(s)	61, 1114
GluI	GCNGC	4 cut(s)	537, 771, 1953, 2169
HaeIII	GGCC	6 cut(s)	550, 1054, 1149, 1906, 2005, 2207
HapII	CCGG	5 cut(s)	665, 825, 1618, 1838, 1897
HhaI	GCGC	2 cut(s)	62, 1115
Hin1I	GRCGYC	1 cut(s)	44
Hin1II	CATG	9 cut(s)	93, 355, 1016, 1319, 1482, 1690, 1927, 1946, 2314
Hin6I	GCGC	2 cut(s)	60, 1113
HinP1I	GCGC	2 cut(s)	60, 1113
HindIII	AAGCTT	2 cut(s)	297, 596
HinfI	GANTC	7 cut(s)	262, 979, 1025, 1340, 1561, 1670, 1783
HpaII	CCGG	5 cut(s)	665, 825, 1618, 1838, 1897
HphI	GGTGA	7 cut(s)	151, 331, 1334, 1450, 1463, 1750, 2087
Hpy166II	GTNNAC	6 cut(s)	583, 901, 1219, 1275, 1396, 1476
Hpy188I	TCNGA	6 cut(s)	532, 1715, 2011, 2064, 2115, 2305
Hpy8I	GTNNAC	6 cut(s)	583, 901, 1219, 1275, 1396, 1476
Hpy99I	CGWCG	1 cut(s)	49
HpyAV	CCTTC	5 cut(s)	439, 571, 673, 2219, 2271
HpyCH4III	ACNGT	8 cut(s)	423, 544, 792, 805, 871, 1040, 1279, 2068
HpyCH4IV	ACGT	3 cut(s)	44, 129, 2226
HpyF10VI	GCNNNNNNNGC	4 cut(s)	506, 1534, 1952, 2213
HpyF3I	CTNAG	6 cut(s)	630, 1049, 1623, 1634, 2039, 2114
HpySE526I	ACGT	3 cut(s)	44, 129, 2226
Hsp92I	GRCGYC	1 cut(s)	44
Hsp92II	CATG	9 cut(s)	93, 355, 1016, 1319, 1482, 1690, 1927, 1946, 2314
HspAI	GCGC	2 cut(s)	60, 1113
Kpn2I	TCCGGA	2 cut(s)	664, 1837
KpnI	GGTACC	2 cut(s)	1012, 1158
Ksp22I	TGATCA	1 cut(s)	1762
LmnI	GCTCC	2 cut(s)	466, 1683
Lsp1109I	GCAGC	3 cut(s)	523, 757, 1939
LweI	GCATC	9 cut(s)	105, 508, 523, 1092, 1116, 1153, 1525, 1939, 2245
MaeI	CTAG	5 cut(s)	228, 341, 711, 2130, 2213
MaeII	ACGT	3 cut(s)	44, 129, 2226
MaeIII	GTNAC	4 cut(s)	169, 223, 2149, 2185
MfeI	CAATTG	2 cut(s)	1287, 1774
MflI	RGATCY	1 cut(s)	821
MhlI	GDGCHC	5 cut(s)	68, 375, 552, 903, 1629
MmeI	TCCRAC	4 cut(s)	273, 414, 1877, 2099
Mph1103I	ATGCAT	1 cut(s)	1539
MroI	TCCGGA	2 cut(s)	664, 1837
MroXI	GAANNNNTTC	3 cut(s)	1339, 1845, 2100
MseI	TTAA	8 cut(s)	453, 615, 735, 915, 1494, 1730, 1802, 2231
MslI	CAYNNNNRTG	7 cut(s)	421, 531, 1314, 1320, 1678, 1947, 2225
MspA1I	CMGCKG	1 cut(s)	1462
MspI	CCGG	5 cut(s)	665, 825, 1618, 1838, 1897
MspR9I	CCNGG	2 cut(s)	1586, 1619
MunI	CAATTG	2 cut(s)	1287, 1774
Mva1269I	GAATGC	1 cut(s)	526
MvaI	CCWGG	1 cut(s)	1586
MvnI	CGCG	1 cut(s)	62
MwoI	GCNNNNNNNGC	4 cut(s)	506, 1534, 1952, 2213
NciI	CCSGG	1 cut(s)	1619
NdeI	CATATG	3 cut(s)	97, 930, 1677
NlaIII	CATG	9 cut(s)	93, 355, 1016, 1319, 1482, 1690, 1927, 1946, 2314
NlaIV	GGNNCC	8 cut(s)	417, 550, 789, 823, 1010, 1156, 1583, 1913
NmeAIII	GCCGAG	3 cut(s)	1175, 1932, 1981
NmuCI	GTSAC	1 cut(s)	169
NsiI	ATGCAT	1 cut(s)	1539
NspI	RCATGY	2 cut(s)	1482, 2314
OliI	CACNNNNGTG	2 cut(s)	421, 1678
PaeI	GCATGC	1 cut(s)	2314
PctI	GAATGC	1 cut(s)	526
PdmI	GAANNNNTTC	3 cut(s)	1339, 1845, 2100
PfeI	GAWTC	7 cut(s)	262, 979, 1025, 1340, 1561, 1670, 1783
PflMI	CCANNNNNTGG	4 cut(s)	56, 600, 1018, 2203
PinAI	ACCGGT	1 cut(s)	1896
PkrI	GCNGC	4 cut(s)	538, 772, 1954, 2170
Ple19I	CGATCG	1 cut(s)	575
Ppu21I	YACGTR	1 cut(s)	2227
PshAI	GACNNNNGTC	2 cut(s)	1305, 2069
PshBI	ATTAAT	1 cut(s)	1494
PsiI	TTATAA	1 cut(s)	1109
Psp124BI	GAGCTC	1 cut(s)	375
Psp6I	CCWGG	1 cut(s)	1584
PspGI	CCWGG	1 cut(s)	1584
PspN4I	GGNNCC	8 cut(s)	417, 550, 789, 823, 1010, 1156, 1583, 1913
PspOMI	GGGCCC	1 cut(s)	548
PspPI	GGNCC	4 cut(s)	548, 549, 1420, 2206
PsuI	RGATCY	1 cut(s)	821
PvuI	CGATCG	1 cut(s)	575
PvuII	CAGCTG	1 cut(s)	1462
RsaI	GTAC	5 cut(s)	1010, 1156, 1250, 1276, 1409
RsaNI	GTAC	5 cut(s)	1009, 1155, 1249, 1275, 1408
RseI	CAYNNNNRTG	7 cut(s)	421, 531, 1314, 1320, 1678, 1947, 2225
SacI	GAGCTC	1 cut(s)	375
SaqAI	TTAA	8 cut(s)	453, 615, 735, 915, 1494, 1730, 1802, 2231
SatI	GCNGC	4 cut(s)	537, 771, 1953, 2169
Sau96I	GGNCC	4 cut(s)	548, 549, 1420, 2206
ScrFI	CCNGG	2 cut(s)	1586, 1619
SduI	GDGCHC	5 cut(s)	68, 375, 552, 903, 1629
SfaNI	GCATC	9 cut(s)	105, 508, 523, 1092, 1116, 1153, 1525, 1939, 2245
SfcI	CTRYAG	1 cut(s)	1036
SinI	GGWCC	1 cut(s)	1420
SmiMI	CAYNNNNRTG	7 cut(s)	421, 531, 1314, 1320, 1678, 1947, 2225
SmlI	CTYRAG	5 cut(s)	21, 495, 920, 1576, 2088
SmoI	CTYRAG	5 cut(s)	21, 495, 920, 1576, 2088
SphI	GCATGC	1 cut(s)	2314
SsiI	CCGC	4 cut(s)	14, 62, 1734, 2169
SspMI	CTAG	5 cut(s)	228, 341, 711, 2130, 2213
SstI	GAGCTC	1 cut(s)	375
StyD4I	CCNGG	2 cut(s)	1584, 1617
TaaI	ACNGT	8 cut(s)	423, 544, 792, 805, 871, 1040, 1279, 2068
TaiI	ACGT	3 cut(s)	47, 132, 2229
TaqI	TCGA	8 cut(s)	195, 289, 571, 575, 1079, 1266, 1569, 2035
TatI	WGTACW	2 cut(s)	1248, 1274
TauI	GCSGC	1 cut(s)	2171
TfiI	GAWTC	7 cut(s)	262, 979, 1025, 1340, 1561, 1670, 1783
Tru1I	TTAA	8 cut(s)	453, 615, 735, 915, 1494, 1730, 1802, 2231
Tru9I	TTAA	8 cut(s)	453, 615, 735, 915, 1494, 1730, 1802, 2231
TscAI	CASTG	6 cut(s)	178, 417, 428, 1473, 1638, 2271
TseFI	GTSAC	1 cut(s)	169
TseI	GCWGC	3 cut(s)	536, 770, 1952
Tsp45I	GTSAC	1 cut(s)	169
TspDTI	ATGAA	9 cut(s)	291, 340, 849, 971, 1038, 1148, 1535, 1796, 1989
TspGWI	ACGGA	1 cut(s)	1003
TspRI	CASTG	6 cut(s)	178, 417, 428, 1473, 1638, 2271
Van91I	CCANNNNNTGG	4 cut(s)	56, 600, 1018, 2203
VneI	GTGCAC	1 cut(s)	899
VpaK11BI	GGWCC	1 cut(s)	1420
VspI	ATTAAT	1 cut(s)	1494
XapI	RAATTY	6 cut(s)	837, 965, 1362, 1455, 1705, 1884
XceI	RCATGY	2 cut(s)	1482, 2314
XmiI	GTMKAC	1 cut(s)	582
XmnI	GAANNNNTTC	3 cut(s)	1339, 1845, 2100
XspI	CTAG	5 cut(s)	228, 341, 711, 2130, 2213
ZraI	GACGTC	1 cut(s)	45
Zsp2I	ATGCAT	1 cut(s)	1539

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.