Rroxscaffold_1G00004010

Multiple C2 and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005951
Physical Location & Seq
Reverse (-)
5476335 .. 5483996
7662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00004010.1

Sequence Viewer

Length: 792 bp
ATGGACACCAGGCTATCTCATGCTGATGCTGCTCATCCTGATGAACGTGACGAAGAGTTTGACACATTCCCAACTTCACGGCCATCAGACATTGTTCGAATGAGGTATGATCGACTGAGAAGTATAGCAGGGAGGGTTCAGACTGTTGTTGGTGACATGGCAACTCAAGGCGAAAGGTTTCAGTCTCTTCTGAGCTGGAGAGACCCAAGAGCAACCACTTTGTTTGTTACTTTCTGTCTGATTCTTGCCATTGTTCTCTATGTGACTCCATTCCAAGTCGTGGCTCTTCTAGCAGGAATTTACATATTAAGGCATCCCAGGTTCCGCCACAAGCTTCCTTCAGCGCCACTCAACTTCTTTAGAAGGTTGCCTGCAAGATCAGACATTGACCGATGGCGTGAAGACAGCGAAACGTGCTTATTTTTTTACATCACGTTTGTAAATATTTCATCGATGGATGTGAAATTGTCCCCACCCGTGGATGTTGCGGACCCCCACTTCGGGCTTCTCTCCCGTATTTTCGGAAGAGTCACCAGACTCCGTGCTTGGCCTTGCTTCATCAACCCCCCAACTCCTCGGCTTTTCGTGTCTCATGAGCGTCTTGTTCTTACCCCACTTCTCAAGGATAAAGCTGTTCAAATTGTGGAAGGTCCGTCGGAATGCCATTGGGATTCTTGGTGCATTATCACCATGAAGAGGTTCGGGACTTGTGTGGAGGACCCTTTGAAGGTTCTGGGAGAATATTTTTGGGGACCAGGGACCAAATACTCAACAACGACAAGAAGTGTGTGA

Protein Analysis

263

Amino Acids

30.35

Weight (kDa)

8.97

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRT_C PF08372 1 - 128 1.4e-67 Plant phosphoribosyltransferase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 280
AciI CCGC 2 cut(s) 325, 488
AcoI YGGCCR 1 cut(s) 80
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 324
AfiI CCNNNNNNNGG 6 cut(s) 280, 478, 500, 501, 696, 727
AgsI TTSAA 2 cut(s) 638, 727
AjnI CCWGG 3 cut(s) 8, 317, 754
AluBI AGCT 3 cut(s) 195, 334, 632
AluI AGCT 3 cut(s) 195, 334, 632
Alw26I GTCTC 3 cut(s) 189, 195, 594
AoxI GGCC 2 cut(s) 80, 548
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 297
ArsI GACNNNNNNTTYG 4 cut(s) 41, 73, 482, 514
Asp700I GAANNNNTTC 2 cut(s) 177, 698
AspLEI GCGC 1 cut(s) 346
AspS9I GGNCC 5 cut(s) 490, 650, 718, 752, 759
AsuHPI GGTGA 3 cut(s) 164, 523, 679
AsuII TTCGAA 1 cut(s) 97
AvaII GGWCC 5 cut(s) 490, 650, 718, 752, 759
BbsI GAAGAC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 16
BccI CCATC 3 cut(s) 91, 387, 448
BceAI ACGGC 1 cut(s) 95
BciT130I CCWGG 3 cut(s) 10, 319, 756
BcoDI GTCTC 3 cut(s) 189, 195, 594
BfaI CTAG 1 cut(s) 290
BfoI RGCGCY 1 cut(s) 347
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 3 cut(s) 10, 319, 756
Bme18I GGWCC 5 cut(s) 490, 650, 718, 752, 759
BmgT120I GGNCC 5 cut(s) 490, 650, 718, 752, 759
BmiI GGNNCC 5 cut(s) 323, 492, 720, 753, 760
BmrFI CCNGG 3 cut(s) 10, 319, 756
BmsI GCATC 2 cut(s) 16, 322
BpiI GAAGAC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 217
Bpu14I TTCGAA 1 cut(s) 97
BpuEI CTTGAG 2 cut(s) 150, 605
Bsa29I ATCGAT 1 cut(s) 452
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 4 cut(s) 317, 477, 575, 755
Bsc4I CCNNNNNNNGG 6 cut(s) 280, 478, 500, 501, 696, 727
BseBI CCWGG 3 cut(s) 10, 319, 756
BseCI ATCGAT 1 cut(s) 452
BseDI CCNNGG 4 cut(s) 317, 477, 575, 755
BseGI GGATG 4 cut(s) 34, 313, 463, 487
BseLI CCNNNNNNNGG 6 cut(s) 280, 478, 500, 501, 696, 727
BseMII CTCAG 2 cut(s) 107, 182
BseRI GAGGAG 1 cut(s) 564
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 2 cut(s) 82, 550
BshVI ATCGAT 1 cut(s) 452
BslFI GGGAC 4 cut(s) 454, 718, 765, 772
BslI CCNNNNNNNGG 6 cut(s) 280, 478, 500, 501, 696, 727
BsmAI GTCTC 3 cut(s) 189, 195, 594
BsmFI GGGAC 4 cut(s) 454, 718, 765, 772
BsmI GAATGC 1 cut(s) 665
BsnI GGCC 2 cut(s) 82, 550
Bso31I GGTCTC 1 cut(s) 195
Bsp119I TTCGAA 1 cut(s) 97
Bsp143I GATC 2 cut(s) 109, 377
BspACI CCGC 2 cut(s) 325, 488
BspANI GGCC 2 cut(s) 82, 550
BspCNI CTCAG 2 cut(s) 108, 183
BspDI ATCGAT 1 cut(s) 452
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 5 cut(s) 323, 492, 720, 753, 760
BspQI GCTCTTC 1 cut(s) 291
BspT104I TTCGAA 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 195
BssECI CCNNGG 4 cut(s) 317, 477, 575, 755
BssMI GATC 2 cut(s) 109, 377
Bst2UI CCWGG 3 cut(s) 10, 319, 756
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 5 cut(s) 48, 192, 291, 520, 689
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 2 cut(s) 116, 191
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 4 cut(s) 34, 313, 463, 487
BstH2I RGCGCY 1 cut(s) 347
BstHHI GCGC 1 cut(s) 346
BstKTI GATC 2 cut(s) 112, 380
BstMAI GTCTC 3 cut(s) 189, 195, 594
BstMBI GATC 2 cut(s) 109, 377
BstMWI GCNNNNNNNGC 3 cut(s) 29, 290, 414
BstNI CCWGG 3 cut(s) 10, 319, 756
BstSCI CCNGG 3 cut(s) 8, 317, 754
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 408
Bsu15I ATCGAT 1 cut(s) 452
BsuRI GGCC 2 cut(s) 82, 550
BsuTUI ATCGAT 1 cut(s) 452
BtgI CCRYGG 1 cut(s) 477
BtsCI GGATG 4 cut(s) 34, 313, 463, 487
Cac8I GCNNGC 1 cut(s) 372
CciI TCATGA 1 cut(s) 592
CfoI GCGC 1 cut(s) 346
Cfr13I GGNCC 5 cut(s) 490, 650, 718, 752, 759
ClaI ATCGAT 1 cut(s) 452
CseI GACGC 1 cut(s) 587
CspCI CAANNNNNGTGG 2 cut(s) 205, 240
CviAII CATG 4 cut(s) 20, 157, 593, 691
CviJI RGCY 9 cut(s) 13, 82, 195, 284, 334, 505, 550, 580, 632
CviKI_1 RGCY 9 cut(s) 13, 82, 195, 284, 334, 505, 550, 580, 632
DdeI CTNAG 2 cut(s) 116, 191
DpnI GATC 2 cut(s) 111, 379
DpnII GATC 2 cut(s) 109, 377
EaeI YGGCCR 1 cut(s) 80
Eam1104I CTCTTC 5 cut(s) 48, 192, 291, 520, 689
EarI CTCTTC 5 cut(s) 48, 192, 291, 520, 689
EciI GGCGGA 1 cut(s) 314
Eco31I GGTCTC 1 cut(s) 195
Eco47I GGWCC 5 cut(s) 490, 650, 718, 752, 759
Eco57I CTGAAG 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 718
EcoRII CCWGG 3 cut(s) 8, 317, 754
FaeI CATG 4 cut(s) 23, 160, 596, 694
FaiI YATR 8 cut(s) 21, 108, 125, 158, 261, 305, 594, 692
FaqI GGGAC 4 cut(s) 454, 718, 765, 772
FatI CATG 4 cut(s) 19, 156, 592, 690
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 4 cut(s) 21, 300, 470, 494
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 1 cut(s) 290
GlaI GCGC 1 cut(s) 345
GluI GCNGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 217
HaeII RGCGCY 1 cut(s) 347
HaeIII GGCC 2 cut(s) 82, 550
HgaI GACGC 1 cut(s) 587
HhaI GCGC 1 cut(s) 346
Hin1II CATG 4 cut(s) 23, 160, 596, 694
Hin6I GCGC 1 cut(s) 344
HinP1I GCGC 1 cut(s) 344
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 5 cut(s) 241, 265, 528, 537, 671
HphI GGTGA 3 cut(s) 164, 523, 679
Hpy188I TCNGA 7 cut(s) 88, 141, 192, 240, 382, 524, 658
Hpy188III TCNNGA 3 cut(s) 38, 593, 703
Hpy99I CGWCG 1 cut(s) 658
HpyAV CCTTC 4 cut(s) 348, 357, 641, 721
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 3 cut(s) 46, 413, 434
HpyCH4V TGCA 2 cut(s) 374, 681
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 290, 414
HpyF3I CTNAG 2 cut(s) 116, 191
HpySE526I ACGT 3 cut(s) 46, 413, 434
Hsp92II CATG 4 cut(s) 23, 160, 596, 694
HspAI GCGC 1 cut(s) 344
Kzo9I GATC 2 cut(s) 109, 377
LguI GCTCTTC 1 cut(s) 291
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 2 cut(s) 16, 322
MaeI CTAG 1 cut(s) 290
MaeII ACGT 3 cut(s) 46, 413, 434
MaeIII GTNAC 5 cut(s) 47, 152, 226, 262, 529
MalI GATC 2 cut(s) 111, 379
MboI GATC 2 cut(s) 109, 377
MboII GAAGA 6 cut(s) 65, 179, 278, 413, 537, 706
MluCI AATT 3 cut(s) 297, 464, 639
MlyI GAGTC 3 cut(s) 259, 531, 537
MmeI TCCRAC 1 cut(s) 636
MnlI CCTC 5 cut(s) 96, 126, 585, 690, 709
MroXI GAANNNNTTC 2 cut(s) 177, 698
MseI TTAA 1 cut(s) 308
MslI CAYNNNNRTG 2 cut(s) 24, 39
MspR9I CCNGG 3 cut(s) 10, 319, 756
Mva1269I GAATGC 1 cut(s) 665
MvaI CCWGG 3 cut(s) 10, 319, 756
MwoI GCNNNNNNNGC 3 cut(s) 29, 290, 414
NdeII GATC 2 cut(s) 109, 377
NlaIII CATG 4 cut(s) 23, 160, 596, 694
NlaIV GGNNCC 5 cut(s) 323, 492, 720, 753, 760
NmeAIII GCCGAG 1 cut(s) 556
NmuCI GTSAC 4 cut(s) 47, 152, 262, 529
NspV TTCGAA 1 cut(s) 97
PagI TCATGA 1 cut(s) 592
PciSI GCTCTTC 1 cut(s) 291
PctI GAATGC 1 cut(s) 665
PdmI GAANNNNTTC 2 cut(s) 177, 698
PfeI GAWTC 2 cut(s) 241, 671
PflMI CCANNNNNTGG 1 cut(s) 280
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 3 cut(s) 259, 531, 536
PpsI GAGTC 3 cut(s) 259, 531, 536
PpuMI RGGWCCY 1 cut(s) 718
Psp5II RGGWCCY 1 cut(s) 718
Psp6I CCWGG 3 cut(s) 8, 317, 754
PspGI CCWGG 3 cut(s) 8, 317, 754
PspN4I GGNNCC 5 cut(s) 323, 492, 720, 753, 760
PspPI GGNCC 5 cut(s) 490, 650, 718, 752, 759
PspPPI RGGWCCY 1 cut(s) 718
RseI CAYNNNNRTG 2 cut(s) 24, 39
SapI GCTCTTC 1 cut(s) 291
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 2 cut(s) 109, 377
Sau96I GGNCC 5 cut(s) 490, 650, 718, 752, 759
SchI GAGTC 3 cut(s) 259, 531, 537
ScrFI CCNGG 3 cut(s) 10, 319, 756
SfaNI GCATC 2 cut(s) 16, 322
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 5 cut(s) 490, 650, 718, 752, 759
SmiMI CAYNNNNRTG 2 cut(s) 24, 39
SmlI CTYRAG 2 cut(s) 165, 620
SmoI CTYRAG 2 cut(s) 165, 620
Sse9I AATT 3 cut(s) 297, 464, 639
SsiI CCGC 2 cut(s) 325, 488
SspI AATATT 2 cut(s) 445, 743
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 3 cut(s) 8, 317, 754
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 3 cut(s) 49, 416, 437
TaqI TCGA 3 cut(s) 97, 112, 452
TaqII GACCGA 1 cut(s) 405
TasI AATT 3 cut(s) 297, 464, 639
TfiI GAWTC 2 cut(s) 241, 671
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TseFI GTSAC 4 cut(s) 47, 152, 262, 529
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 4 cut(s) 47, 152, 262, 529
TspDTI ATGAA 4 cut(s) 57, 438, 547, 707
TspGWI ACGGA 2 cut(s) 530, 642
Van91I CCANNNNNTGG 1 cut(s) 280
VpaK11BI GGWCC 5 cut(s) 490, 650, 718, 752, 759
XapI RAATTY 1 cut(s) 297
XmnI GAANNNNTTC 2 cut(s) 177, 698
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.