Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010942

Physical Location & Seq

Genomic Coordinates Reverse (-)

1926441 .. 1927673

Gene Structure 1233 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1023600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1233 bp

ATGGACGACTCGCCCTTACCTACAGAAACCTCGCCGGAGCTCGATCTCGAAAACCTCAGGGCCCTCAAAGTCTTAGGCAAGGGAGCAATGGGCACCGTTTTCTTAGTCCACGACCCGTCCTCTGACCCCTCCGCCCGCCGCCCGTTCGCTCTCAAAGTCGTTGACAAGTACTCGCTCCGCTCCAAGCTTGACGCCGAGCGCCGCGCCCGTTGGGAAATACAGGTCCTGACCCGACTATCCGCCCCGATCCCGCACCCGTTTCTGCCCTCCATCATGGGGTCCTTCGAGTCCGACGAGTTCATGGGATGGGCCGTCCCTTACTGTTCCGGCGGCGACCTTAACGTCCTCCGGTACCGGCAAAACGACCGCGTTTTTTCCCCCGCCGTGATCCGATTTTACTTGGCGGAGATCGTTTGCGCGCTCGACCACCTCCACAGCATGGGAATCGCCTACCGAGATTTAAAGCCCGAAAATGTCCTCATCCAGCAATCCGGTCACTTGACACTCACAGACTTCGACCTCTCGATTAGCCTCAAGCACCGGACCGCCAAGCCCAGCGACTCCGTTCCGGAAAACGACGTCCCGGAGATTCGCCGCAAACACCGGCGGAACTTGACACGTTGGATCACGATTATAAACGACAATCACAAGAACGGCGGCAGCGGGAAGGGGTTGAAGAAGGCCAAGTCGGCCCGAGTCAGCCCGATGAGTCGACGGAAGCTGAGTTTCTCGGACGGCGAACGCGCGAACTCGTTTGTCGGAACGGAAGAGTACGTATCCCCCGAGGTGGTGCGCGGGGAGGGCCACGAGTTCGCCGTTGACTGGTGGGCCCTCGGAATTCTAACTTACGAGGCGTTGTACGGTACGACGCCGTTCAAGGGGAAGAATCGGAAAGAGACATTTGGGAACGTCCTGACGAAGACGCCTGAGTTTATCGGGAAGCGGACGGCGCTAACGGACTTGATCGAGAGGCTGTTAAACAAGGACCCGATCAAGAGGCTAGGGTACCACCGGGGCGCGTGCGAGATCAAGGAGCACGAGTTCTTTCGCGGGGTCCGGTGGGAACTGCTGACGGAGGTTTCGCGGCCACCGTGCATTCCGTCGCGGGAGGATGGTGACGTAACGGATAAAACAACGGCTGGAGGGAGAGTGAGCGTGAGGGAGTATTTTCAAAAGTTACGGTCTCCCCCCTCGGTGCCGCCATCGCCGGATTGTCAACTAACGGAGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0009611 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0035556 GO:0035670 GO:0036211 GO:0036290 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051302 GO:0051716 GO:0051782 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0080060 GO:0090567 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

411

Amino Acids

46.34

Weight (kDa)

9.03

Isoelectric Point (pI)

52.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	21 - 182	5e-12	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	22 - 183	6e-29	Protein kinase domain
Pkinase	PF00069	245 - 349	3.8e-19	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010942)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51170 AT3G20830
fragaria_vesca	FvH4_3g43220
malus_domestica	MD03G1023600.v1.1 MD11G1028700.v1.1
prunus_persica	Prupe.6G022800_v2.0.a1
pyrus_communis	pycom03g02020 pycom08g08880 pycom11g02220 pycom13g22320
rosa_chinensis	RchiOBHm_Chr5g0077231
rosa_laevigata	RLG00000036675
rosa_multiflora	Rmu_sc0005576.1_g000006
rosa_roxburghii	Rroxscaffold_1G00004780
rosa_rugosa	Rorug05G0453000
rosa_samantha	Rh5AG508000 Rh5BG529400 Rh5CG553800 Rh5DG541700
rosa_wichuraiana	Rw5G046950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	635
AasI	GACNNNNNNGTC	1 cut(s)	341
AatII	GACGTC	1 cut(s)	582
Acc65I	GGTACC	2 cut(s)	351, 1005
AccB1I	GGYRCC	4 cut(s)	92, 351, 1005, 1195
AccB7I	CCANNNNNTGG	1 cut(s)	439
AccBSI	CCGCTC	1 cut(s)	180
AccI	GTMKAC	1 cut(s)	712
AccIII	TCCGGA	1 cut(s)	568
AclWI	GGATC	3 cut(s)	241, 382, 632
AcoI	YGGCCR	1 cut(s)	1085
AcsI	RAATTY	1 cut(s)	837
AcyI	GRCGYC	4 cut(s)	192, 579, 869, 923
AfaI	GTAC	6 cut(s)	170, 353, 773, 860, 865, 1007
AfiI	CCNNNNNNNGG	5 cut(s)	276, 439, 787, 822, 878
AflIII	ACRYGT	1 cut(s)	617
AgsI	TTSAA	3 cut(s)	676, 877, 1172
AloI	GAACNNNNNNTCC	2 cut(s)	1025, 1057
AluBI	AGCT	3 cut(s)	40, 187, 721
AluI	AGCT	3 cut(s)	40, 187, 721
Alw21I	GWGCWC	2 cut(s)	42, 1038
Alw26I	GTCTC	2 cut(s)	890, 1188
AlwI	GGATC	3 cut(s)	241, 382, 632
AlwNI	CAGNNNCTG	1 cut(s)	226
Ama87I	CYCGRG	2 cut(s)	693, 782
Aor13HI	TCCGGA	1 cut(s)	568
AoxI	GGCC	7 cut(s)	60, 309, 681, 690, 802, 828, 1085
ApaI	GGGCCC	2 cut(s)	64, 832
ApeKI	GCWGC	1 cut(s)	660
ApoI	RAATTY	1 cut(s)	837
ArsI	GACNNNNNNTTYG	2 cut(s)	1063, 1095
Asp718I	GGTACC	2 cut(s)	351, 1005
AspLEI	GCGC	8 cut(s)	201, 206, 419, 421, 746, 795, 952, 1019
AsuC2I	CCSGG	2 cut(s)	584, 1013
AsuHPI	GGTGA	1 cut(s)	1127
AvaI	CYCGRG	2 cut(s)	693, 782
AvaII	GGWCC	5 cut(s)	223, 279, 543, 985, 1054
AxyI	CCTNAGG	1 cut(s)	56
BaeGI	GKGCMC	3 cut(s)	64, 95, 832
BanI	GGYRCC	4 cut(s)	92, 351, 1005, 1195
BanII	GRGCYC	3 cut(s)	42, 64, 832
BauI	CACGAG	2 cut(s)	806, 1037
BbsI	GAAGAC	1 cut(s)	926
Bbv12I	GWGCWC	2 cut(s)	42, 1038
BbvI	GCAGC	1 cut(s)	672
BccI	CCATC	4 cut(s)	278, 300, 1106, 1210
BceAI	ACGGC	8 cut(s)	296, 368, 670, 751, 800, 856, 963, 1152
BcgI	CGANNNNNNTGC	2 cut(s)	427, 461
BciVI	GTATCC	1 cut(s)	787
BcnI	CCSGG	2 cut(s)	584, 1013
BcoDI	GTCTC	2 cut(s)	890, 1188
BfaI	CTAG	1 cut(s)	1001
BfmI	CTRYAG	1 cut(s)	21
BfoI	RGCGCY	2 cut(s)	202, 953
BfuI	GTATCC	1 cut(s)	787
BglI	GCCNNNNNGGC	1 cut(s)	689
BisI	GCNGC	8 cut(s)	139, 202, 331, 595, 658, 661, 1085, 1199
BlsI	GCNGC	8 cut(s)	140, 203, 332, 596, 659, 662, 1086, 1200
BmcAI	AGTACT	1 cut(s)	170
Bme1390I	CCNGG	2 cut(s)	584, 1013
Bme18I	GGWCC	5 cut(s)	223, 279, 543, 985, 1054
BmeT110I	CYCGRG	2 cut(s)	693, 782
BmiI	GGNNCC	9 cut(s)	62, 94, 280, 353, 830, 987, 1007, 1055, 1197
BmrFI	CCNGG	2 cut(s)	584, 1013
BpiI	GAAGAC	1 cut(s)	926
BpmI	CTGGAG	1 cut(s)	1161
BpuEI	CTTGAG	1 cut(s)	518
BpuMI	CCSGG	2 cut(s)	584, 1013
BsaAI	YACGTR	1 cut(s)	775
BsaHI	GRCGYC	4 cut(s)	192, 579, 869, 923
BsaI	GGTCTC	1 cut(s)	1188
BsaJI	CCNNGG	4 cut(s)	783, 832, 1012, 1191
BsaWI	WCCGGW	5 cut(s)	348, 491, 540, 568, 1056
BsaXI	ACNNNNNCTCC	4 cut(s)	1025, 1055, 1134, 1164
Bsc4I	CCNNNNNNNGG	5 cut(s)	276, 439, 787, 822, 878
Bse118I	RCCGGY	2 cut(s)	354, 603
Bse1I	ACTGG	1 cut(s)	827
Bse21I	CCTNAGG	1 cut(s)	56
Bse3DI	GCAATG	1 cut(s)	93
BseAI	TCCGGA	1 cut(s)	568
BseDI	CCNNGG	4 cut(s)	783, 832, 1012, 1191
BseGI	GGATG	3 cut(s)	311, 480, 1117
BseLI	CCNNNNNNNGG	5 cut(s)	276, 439, 787, 822, 878
BseMI	GCAATG	1 cut(s)	93
BseMII	CTCAG	3 cut(s)	70, 713, 918
BseNI	ACTGG	1 cut(s)	827
BsePI	GCGCGC	1 cut(s)	417
BseSI	GKGCMC	3 cut(s)	64, 95, 832
BseXI	GCAGC	1 cut(s)	672
BseYI	CCCAGC	1 cut(s)	554
Bsh1285I	CGRYCG	1 cut(s)	367
BshFI	GGCC	7 cut(s)	62, 311, 683, 692, 804, 830, 1087
BshNI	GGYRCC	4 cut(s)	92, 351, 1005, 1195
BsiEI	CGRYCG	1 cut(s)	367
BsiHKAI	GWGCWC	2 cut(s)	42, 1038
BsiHKCI	CYCGRG	2 cut(s)	693, 782
BslFI	GGGAC	2 cut(s)	299, 566
BslI	CCNNNNNNNGG	5 cut(s)	276, 439, 787, 822, 878
BsmAI	GTCTC	2 cut(s)	890, 1188
BsmFI	GGGAC	2 cut(s)	299, 566
BsmI	GAATGC	1 cut(s)	1095
BsnI	GGCC	7 cut(s)	62, 311, 683, 692, 804, 830, 1087
Bso31I	GGTCTC	1 cut(s)	1188
BsoBI	CYCGRG	2 cut(s)	693, 782
Bsp120I	GGGCCC	2 cut(s)	60, 828
Bsp1286I	GDGCHC	5 cut(s)	42, 64, 95, 832, 1038
Bsp13I	TCCGGA	1 cut(s)	568
Bsp143I	GATC	8 cut(s)	43, 246, 387, 408, 624, 963, 990, 1026
BspANI	GGCC	7 cut(s)	62, 311, 683, 692, 804, 830, 1087
BspCNI	CTCAG	3 cut(s)	69, 714, 919
BspEI	TCCGGA	1 cut(s)	568
BspLI	GGNNCC	9 cut(s)	62, 94, 280, 353, 830, 987, 1007, 1055, 1197
BspPI	GGATC	3 cut(s)	241, 382, 632
BspT107I	GGYRCC	4 cut(s)	92, 351, 1005, 1195
BspTNI	GGTCTC	1 cut(s)	1188
BsrBI	CCGCTC	1 cut(s)	180
BsrDI	GCAATG	1 cut(s)	93
BsrFI	RCCGGY	2 cut(s)	354, 603
BsrI	ACTGG	1 cut(s)	827
BssAI	RCCGGY	2 cut(s)	354, 603
BssECI	CCNNGG	4 cut(s)	783, 832, 1012, 1191
BssHII	GCGCGC	1 cut(s)	417
BssMI	GATC	8 cut(s)	43, 246, 387, 408, 624, 963, 990, 1026
BssNI	GRCGYC	4 cut(s)	192, 579, 869, 923
BssSI	CACGAG	2 cut(s)	806, 1037
Bst2BI	CACGAG	2 cut(s)	806, 1037
Bst4CI	ACNGT	5 cut(s)	97, 323, 863, 1092, 1182
Bst6I	CTCTTC	1 cut(s)	762
BstACI	GRCGYC	4 cut(s)	192, 579, 869, 923
BstBAI	YACGTR	1 cut(s)	775
BstC8I	GCNNGC	3 cut(s)	136, 419, 1021
BstDEI	CTNAG	5 cut(s)	56, 73, 103, 722, 927
BstF5I	GGATG	3 cut(s)	311, 480, 1117
BstH2I	RGCGCY	2 cut(s)	202, 953
BstHHI	GCGC	8 cut(s)	201, 206, 419, 421, 746, 795, 952, 1019
BstKTI	GATC	8 cut(s)	46, 249, 390, 411, 627, 966, 993, 1029
BstMAI	GTCTC	2 cut(s)	890, 1188
BstMBI	GATC	8 cut(s)	43, 246, 387, 408, 624, 963, 990, 1026
BstMCI	CGRYCG	1 cut(s)	367
BstMWI	GCNNNNNNNGC	4 cut(s)	689, 801, 949, 1204
BstSCI	CCNGG	2 cut(s)	582, 1011
BstSFI	CTRYAG	1 cut(s)	21
BstSLI	GKGCMC	3 cut(s)	64, 95, 832
BstSNI	TACGTA	1 cut(s)	775
BstV1I	GCAGC	1 cut(s)	672
BstV2I	GAAGAC	1 cut(s)	926
Bsu36I	CCTNAGG	1 cut(s)	56
BsuI	GTATCC	1 cut(s)	787
BsuRI	GGCC	7 cut(s)	62, 311, 683, 692, 804, 830, 1087
BtgZI	GCGATG	1 cut(s)	1188
BtsCI	GGATG	3 cut(s)	311, 480, 1117
Cac8I	GCNNGC	3 cut(s)	136, 419, 1021
CaiI	CAGNNNCTG	1 cut(s)	226
CfoI	GCGC	8 cut(s)	201, 206, 419, 421, 746, 795, 952, 1019
Cfr10I	RCCGGY	2 cut(s)	354, 603
CpoI	CGGWCCG	1 cut(s)	543
CseI	GACGC	3 cut(s)	200, 877, 931
Csp6I	GTAC	6 cut(s)	169, 352, 772, 859, 864, 1006
CspI	CGGWCCG	1 cut(s)	543
CviAII	CATG	3 cut(s)	274, 301, 439
CviQI	GTAC	6 cut(s)	169, 352, 772, 859, 864, 1006
DdeI	CTNAG	5 cut(s)	56, 73, 103, 722, 927
DpnI	GATC	8 cut(s)	45, 248, 389, 410, 626, 965, 992, 1028
DpnII	GATC	8 cut(s)	43, 246, 387, 408, 624, 963, 990, 1026
DraI	TTTAAA	1 cut(s)	462
DrdI	GACNNNNNNGTC	1 cut(s)	341
DseDI	GACNNNNNNGTC	1 cut(s)	341
EaeI	YGGCCR	1 cut(s)	1085
Eam1104I	CTCTTC	1 cut(s)	762
EarI	CTCTTC	1 cut(s)	762
EciI	GGCGGA	4 cut(s)	121, 229, 419, 622
Ecl136II	GAGCTC	1 cut(s)	40
Eco105I	TACGTA	1 cut(s)	775
Eco24I	GRGCYC	3 cut(s)	42, 64, 832
Eco31I	GGTCTC	1 cut(s)	1188
Eco47I	GGWCC	5 cut(s)	223, 279, 543, 985, 1054
Eco53kI	GAGCTC	1 cut(s)	40
Eco81I	CCTNAGG	1 cut(s)	56
Eco88I	CYCGRG	2 cut(s)	693, 782
EcoICRI	GAGCTC	1 cut(s)	40
EcoO109I	RGGNCCY	6 cut(s)	60, 61, 223, 279, 829, 985
EcoRI	GAATTC	1 cut(s)	837
EcoT38I	GRGCYC	3 cut(s)	42, 64, 832
FaeI	CATG	3 cut(s)	277, 304, 442
FaiI	YATR	4 cut(s)	275, 302, 440, 635
FaqI	GGGAC	2 cut(s)	299, 566
FatI	CATG	3 cut(s)	273, 300, 438
FauI	CCCGC	7 cut(s)	143, 258, 388, 656, 788, 1043, 1098
FblI	GTMKAC	1 cut(s)	712
Fnu4HI	GCNGC	8 cut(s)	139, 202, 331, 595, 658, 661, 1085, 1199
FokI	GGATG	3 cut(s)	318, 467, 1124
FriOI	GRGCYC	3 cut(s)	42, 64, 832
Fsp4HI	GCNGC	8 cut(s)	139, 202, 331, 595, 658, 661, 1085, 1199
FspBI	CTAG	1 cut(s)	1001
GlaI	GCGC	8 cut(s)	200, 205, 418, 420, 745, 794, 951, 1018
GluI	GCNGC	8 cut(s)	139, 202, 331, 595, 658, 661, 1085, 1199
GsaI	CCCAGC	1 cut(s)	558
GsuI	CTGGAG	1 cut(s)	1161
HaeII	RGCGCY	2 cut(s)	202, 953
HaeIII	GGCC	7 cut(s)	62, 311, 683, 692, 804, 830, 1087
HgaI	GACGC	3 cut(s)	200, 877, 931
HhaI	GCGC	8 cut(s)	201, 206, 419, 421, 746, 795, 952, 1019
Hin1I	GRCGYC	4 cut(s)	192, 579, 869, 923
Hin1II	CATG	3 cut(s)	277, 304, 442
Hin6I	GCGC	8 cut(s)	199, 204, 417, 419, 744, 793, 950, 1017
HinP1I	GCGC	8 cut(s)	199, 204, 417, 419, 744, 793, 950, 1017
HincII	GTYRAC	4 cut(s)	163, 713, 820, 1217
HindII	GTYRAC	4 cut(s)	163, 713, 820, 1217
HindIII	AAGCTT	1 cut(s)	185
HinfI	GANTC	8 cut(s)	8, 287, 444, 560, 589, 696, 709, 886
HphI	GGTGA	1 cut(s)	1127
Hpy166II	GTNNAC	5 cut(s)	109, 163, 713, 820, 1217
Hpy188I	TCNGA	8 cut(s)	124, 292, 392, 733, 761, 836, 891, 1232
Hpy188III	TCNNGA	9 cut(s)	47, 226, 523, 569, 628, 913, 937, 967, 994
Hpy8I	GTNNAC	5 cut(s)	109, 163, 713, 820, 1217
Hpy99I	CGWCG	5 cut(s)	296, 581, 717, 871, 1105
HpyAV	CCTTC	3 cut(s)	292, 661, 673
HpyCH4III	ACNGT	5 cut(s)	97, 323, 863, 1092, 1182
HpyCH4IV	ACGT	6 cut(s)	342, 579, 619, 774, 909, 1119
HpyCH4V	TGCA	1 cut(s)	1095
HpyF10VI	GCNNNNNNNGC	4 cut(s)	689, 801, 949, 1204
HpyF3I	CTNAG	5 cut(s)	56, 73, 103, 722, 927
HpySE526I	ACGT	6 cut(s)	342, 579, 619, 774, 909, 1119
Hsp92I	GRCGYC	4 cut(s)	192, 579, 869, 923
Hsp92II	CATG	3 cut(s)	277, 304, 442
HspAI	GCGC	8 cut(s)	199, 204, 417, 419, 744, 793, 950, 1017
Kpn2I	TCCGGA	1 cut(s)	568
KpnI	GGTACC	2 cut(s)	355, 1009
Kzo9I	GATC	8 cut(s)	43, 246, 387, 408, 624, 963, 990, 1026
LmnI	GCTCC	5 cut(s)	37, 83, 180, 185, 1033
Lsp1109I	GCAGC	1 cut(s)	672
MaeI	CTAG	1 cut(s)	1001
MaeII	ACGT	6 cut(s)	342, 579, 619, 774, 909, 1119
MaeIII	GTNAC	4 cut(s)	494, 1115, 1120, 1176
MalI	GATC	8 cut(s)	45, 248, 389, 410, 626, 965, 992, 1028
MbiI	CCGCTC	1 cut(s)	180
MboI	GATC	8 cut(s)	43, 246, 387, 408, 624, 963, 990, 1026
MboII	GAAGA	4 cut(s)	688, 779, 895, 931
MhlI	GDGCHC	5 cut(s)	42, 64, 95, 832, 1038
MluCI	AATT	1 cut(s)	837
MlyI	GAGTC	5 cut(s)	2, 296, 554, 705, 718
MmeI	TCCRAC	3 cut(s)	315, 602, 739
MroI	TCCGGA	1 cut(s)	568
MseI	TTAA	3 cut(s)	339, 461, 977
MspA1I	CMGCKG	1 cut(s)	663
MspR9I	CCNGG	2 cut(s)	584, 1013
MteI	GCGCNGCGC	1 cut(s)	202
Mva1269I	GAATGC	1 cut(s)	1095
MwoI	GCNNNNNNNGC	4 cut(s)	689, 801, 949, 1204
NciI	CCSGG	2 cut(s)	584, 1013
NdeII	GATC	8 cut(s)	43, 246, 387, 408, 624, 963, 990, 1026
NlaIII	CATG	3 cut(s)	277, 304, 442
NlaIV	GGNNCC	9 cut(s)	62, 94, 280, 353, 830, 987, 1007, 1055, 1197
NmeAIII	GCCGAG	1 cut(s)	220
NmuCI	GTSAC	2 cut(s)	494, 1115
PauI	GCGCGC	1 cut(s)	417
PcsI	WCGNNNNNNNCGW	4 cut(s)	291, 780, 813, 1088
PctI	GAATGC	1 cut(s)	1095
PfeI	GAWTC	3 cut(s)	444, 589, 886
PflMI	CCANNNNNTGG	1 cut(s)	439
PfoI	TCCNGGA	1 cut(s)	582
PkrI	GCNGC	8 cut(s)	140, 203, 332, 596, 659, 662, 1086, 1200
PleI	GAGTC	5 cut(s)	2, 295, 554, 704, 717
PpsI	GAGTC	5 cut(s)	2, 295, 554, 704, 717
Ppu21I	YACGTR	1 cut(s)	775
PpuMI	RGGWCCY	3 cut(s)	223, 279, 985
PsiI	TTATAA	1 cut(s)	635
Psp124BI	GAGCTC	1 cut(s)	42
Psp5II	RGGWCCY	3 cut(s)	223, 279, 985
PspFI	CCCAGC	1 cut(s)	554
PspN4I	GGNNCC	9 cut(s)	62, 94, 280, 353, 830, 987, 1007, 1055, 1197
PspOMI	GGGCCC	2 cut(s)	60, 828
PspPPI	RGGWCCY	3 cut(s)	223, 279, 985
PstNI	CAGNNNCTG	1 cut(s)	226
PteI	GCGCGC	1 cut(s)	417
RsaI	GTAC	6 cut(s)	170, 353, 773, 860, 865, 1007
RsaNI	GTAC	6 cut(s)	169, 352, 772, 859, 864, 1006
Rsr2I	CGGWCCG	1 cut(s)	543
RsrII	CGGWCCG	1 cut(s)	543
SacI	GAGCTC	1 cut(s)	42
SalI	GTCGAC	1 cut(s)	711
SaqAI	TTAA	3 cut(s)	339, 461, 977
SatI	GCNGC	8 cut(s)	139, 202, 331, 595, 658, 661, 1085, 1199
Sau3AI	GATC	8 cut(s)	43, 246, 387, 408, 624, 963, 990, 1026
ScaI	AGTACT	1 cut(s)	170
SchI	GAGTC	5 cut(s)	2, 296, 554, 705, 718
ScrFI	CCNGG	2 cut(s)	584, 1013
SduI	GDGCHC	5 cut(s)	42, 64, 95, 832, 1038
SfcI	CTRYAG	1 cut(s)	21
SfiI	GGCCNNNNNGGCC	1 cut(s)	689
SgrAI	CRCCGGYG	1 cut(s)	603
SinI	GGWCC	5 cut(s)	223, 279, 543, 985, 1054
SmlI	CTYRAG	1 cut(s)	533
SmoI	CTYRAG	1 cut(s)	533
SnaBI	TACGTA	1 cut(s)	775
Sse9I	AATT	1 cut(s)	837
SspMI	CTAG	1 cut(s)	1001
SstI	GAGCTC	1 cut(s)	42
StyD4I	CCNGG	2 cut(s)	582, 1011
TaaI	ACNGT	5 cut(s)	97, 323, 863, 1092, 1182
TaiI	ACGT	6 cut(s)	345, 582, 622, 777, 912, 1122
TaqI	TCGA	8 cut(s)	42, 48, 285, 423, 516, 524, 712, 966
TasI	AATT	1 cut(s)	837
TatI	WGTACW	1 cut(s)	168
TauI	GCSGC	7 cut(s)	141, 204, 333, 597, 660, 1087, 1201
TfiI	GAWTC	3 cut(s)	444, 589, 886
Tru1I	TTAA	3 cut(s)	339, 461, 977
Tru9I	TTAA	3 cut(s)	339, 461, 977
TseFI	GTSAC	2 cut(s)	494, 1115
TseI	GCWGC	1 cut(s)	660
Tsp45I	GTSAC	2 cut(s)	494, 1115
TspDTI	ATGAA	1 cut(s)	289
TspGWI	ACGGA	7 cut(s)	553, 730, 779, 971, 1088, 1089, 1139
Van91I	CCANNNNNTGG	1 cut(s)	439
VpaK11BI	GGWCC	5 cut(s)	223, 279, 543, 985, 1054
XapI	RAATTY	1 cut(s)	837
XmiI	GTMKAC	1 cut(s)	712
XspI	CTAG	1 cut(s)	1001
ZraI	GACGTC	1 cut(s)	580
ZrmI	AGTACT	1 cut(s)	170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.