MD03G1028600.v1.1

RPM1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008210
Physical Location & Seq
Forward (+)
2341778 .. 2345002
3225 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1028600.v1.1.491

Sequence Viewer

Length: 246 bp
ATGTCGGAAAAAGGCAGGCCACTCCCAAAATTTGGTGAATGGGACGTCAATGATCCTGCTTCAGCTGAGGGGTTTACTGTGATCTTTAACAAGGCTAGGGATGAGAAGAAGACAGGTGGAAAACCCGAGTCACCAGGAAAGGCTGATCCTAATATCAGGCAGGGAGGAGGAGTGGATCCTGGCACTGGCCGGCCTTCATCTAAAAAATGGTTTTGCTGCCTTCATCAACCACAGACCGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

82

Amino Acids

8.77

Weight (kDa)

8.66

Isoelectric Point (pI)

56.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 3 - 37 2.8e-20 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 48
AccB7I CCANNNNNTGG 1 cut(s) 32
AclWI GGATC 4 cut(s) 47, 140, 170, 183
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 1 cut(s) 29
AcuI CTGAAG 1 cut(s) 45
AcyI GRCGYC 1 cut(s) 45
AfiI CCNNNNNNNGG 2 cut(s) 32, 185
AjnI CCWGG 2 cut(s) 133, 178
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
AlwI GGATC 4 cut(s) 47, 140, 170, 183
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 3 cut(s) 17, 187, 191
ApeKI GCWGC 1 cut(s) 216
ApoI RAATTY 1 cut(s) 29
AsuHPI GGTGA 2 cut(s) 47, 123
AvaI CYCGRG 1 cut(s) 125
BamHI GGATCC 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 116
BbvCI CCTCAGC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 203
BciT130I CCWGG 2 cut(s) 135, 180
BfaI CTAG 1 cut(s) 96
BisI GCNGC 1 cut(s) 217
BlsI GCNGC 1 cut(s) 218
Bme1390I CCNGG 2 cut(s) 135, 180
BmeT110I CYCGRG 1 cut(s) 125
BmiI GGNNCC 1 cut(s) 177
BmrFI CCNGG 2 cut(s) 135, 180
BpiI GAAGAC 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 185
Bse118I RCCGGY 1 cut(s) 189
Bse1I ACTGG 1 cut(s) 190
BseBI CCWGG 2 cut(s) 135, 180
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 2 cut(s) 32, 185
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 1 cut(s) 190
BseRI GAGGAG 2 cut(s) 180, 183
BseXI GCAGC 1 cut(s) 203
BshFI GGCC 3 cut(s) 19, 189, 193
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 1 cut(s) 190
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 32, 185
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 3 cut(s) 19, 189, 193
BsoBI CYCGRG 1 cut(s) 125
Bsp143I GATC 4 cut(s) 52, 81, 145, 175
BspANI GGCC 3 cut(s) 19, 189, 193
BspCNI CTCAG 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 177
BspPI GGATC 4 cut(s) 47, 140, 170, 183
BsrFI RCCGGY 1 cut(s) 189
BsrI ACTGG 1 cut(s) 190
BssAI RCCGGY 1 cut(s) 189
BssMI GATC 4 cut(s) 52, 81, 145, 175
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 2 cut(s) 135, 180
Bst4CI ACNGT 1 cut(s) 79
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 17, 191
BstDEI CTNAG 1 cut(s) 66
BstF5I GGATG 1 cut(s) 106
BstKTI GATC 4 cut(s) 55, 84, 148, 178
BstMBI GATC 4 cut(s) 52, 81, 145, 175
BstNI CCWGG 2 cut(s) 135, 180
BstSCI CCNGG 2 cut(s) 133, 178
BstV1I GCAGC 1 cut(s) 203
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuRI GGCC 3 cut(s) 19, 189, 193
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 2 cut(s) 17, 191
Cfr10I RCCGGY 1 cut(s) 189
CviJI RGCY 6 cut(s) 19, 65, 95, 143, 189, 193
CviKI_1 RGCY 6 cut(s) 19, 65, 95, 143, 189, 193
DdeI CTNAG 1 cut(s) 66
DpnI GATC 4 cut(s) 54, 83, 147, 177
DpnII GATC 4 cut(s) 52, 81, 145, 175
EaeI YGGCCR 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 45
Eco88I CYCGRG 1 cut(s) 125
EcoRII CCWGG 2 cut(s) 133, 178
FaqI GGGAC 1 cut(s) 56
Fnu4HI GCNGC 1 cut(s) 217
FokI GGATG 1 cut(s) 113
FseI GGCCGGCC 1 cut(s) 193
Fsp4HI GCNGC 1 cut(s) 217
FspBI CTAG 1 cut(s) 96
GluI GCNGC 1 cut(s) 217
HaeIII GGCC 3 cut(s) 19, 189, 193
HapII CCGG 1 cut(s) 190
Hin1I GRCGYC 1 cut(s) 45
HinfI GANTC 2 cut(s) 128, 239
HpaII CCGG 1 cut(s) 190
HphI GGTGA 2 cut(s) 47, 123
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 1 cut(s) 7
Hpy188III TCNNGA 1 cut(s) 243
Hpy8I GTNNAC 1 cut(s) 75
HpyAV CCTTC 2 cut(s) 204, 230
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 1 cut(s) 45
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 45
KroI GCCGGC 1 cut(s) 189
KroNI GCCGGC 1 cut(s) 191
Kzo9I GATC 4 cut(s) 52, 81, 145, 175
Lsp1109I GCAGC 1 cut(s) 203
MaeI CTAG 1 cut(s) 96
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 129
MalI GATC 4 cut(s) 54, 83, 147, 177
MboI GATC 4 cut(s) 52, 81, 145, 175
MboII GAAGA 2 cut(s) 118, 121
MflI RGATCY 1 cut(s) 175
MluCI AATT 1 cut(s) 29
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 3 cut(s) 61, 158, 161
MroNI GCCGGC 1 cut(s) 189
MseI TTAA 1 cut(s) 87
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 1 cut(s) 190
MspR9I CCNGG 2 cut(s) 135, 180
MvaI CCWGG 2 cut(s) 135, 180
NaeI GCCGGC 1 cut(s) 191
NdeII GATC 4 cut(s) 52, 81, 145, 175
NgoMIV GCCGGC 1 cut(s) 189
NlaIV GGNNCC 1 cut(s) 177
NmuCI GTSAC 1 cut(s) 129
PdiI GCCGGC 1 cut(s) 191
PflFI GACNNNGTC 1 cut(s) 238
PflMI CCANNNNNTGG 1 cut(s) 32
PkrI GCNGC 1 cut(s) 218
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
Psp6I CCWGG 2 cut(s) 133, 178
PspGI CCWGG 2 cut(s) 133, 178
PspN4I GGNNCC 1 cut(s) 177
PsuI RGATCY 1 cut(s) 175
PsyI GACNNNGTC 1 cut(s) 238
PvuII CAGCTG 1 cut(s) 65
RigI GGCCGGCC 1 cut(s) 193
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 1 cut(s) 217
Sau3AI GATC 4 cut(s) 52, 81, 145, 175
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 135, 180
SetI ASST 3 cut(s) 48, 67, 118
Sse9I AATT 1 cut(s) 29
SspMI CTAG 1 cut(s) 96
StyD4I CCNGG 2 cut(s) 133, 178
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 1 cut(s) 48
TasI AATT 1 cut(s) 29
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 129
TseI GCWGC 1 cut(s) 216
Tsp45I GTSAC 1 cut(s) 129
TspDTI ATGAA 2 cut(s) 186, 212
TspRI CASTG 1 cut(s) 190
Tth111I GACNNNGTC 1 cut(s) 238
Van91I CCANNNNNTGG 1 cut(s) 32
XapI RAATTY 1 cut(s) 29
XspI CTAG 1 cut(s) 96
ZraI GACGTC 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.