MD11G1032200.v1.1

RPM1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008210
Physical Location & Seq
Forward (+)
2816591 .. 2819725
3135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1032200.v1.1.491

Sequence Viewer

Length: 246 bp
ATGTCGGAAAAAGGCAGGCCACTCCCAAAATTTGGTGAATGGGACGTCAATGATCCTGCTTCGGCTGAGGGGTTTACTGTGATCTTTAACAAGGCTAGGGATGAGAAGAAGACAGGTGGAAAACCCGAGTCACCAGGAAAGGTTGATCCTAATATCAGGCATGGAGGAGGAGTGGATCCTGGCACTGGCAGGCCTTCATCTAAAAAATGGTTTTGCTGCATTCATCAACCACCGGCAGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

82

Amino Acids

8.75

Weight (kDa)

8.66

Isoelectric Point (pI)

52.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 3 - 37 2.8e-20 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 48
AccB7I CCANNNNNTGG 1 cut(s) 32
AclWI GGATC 4 cut(s) 47, 140, 170, 183
AcsI RAATTY 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 45
AfiI CCNNNNNNNGG 2 cut(s) 32, 185
AjnI CCWGG 2 cut(s) 133, 178
AlwI GGATC 4 cut(s) 47, 140, 170, 183
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 2 cut(s) 17, 191
ApeKI GCWGC 1 cut(s) 216
ApoI RAATTY 1 cut(s) 29
AsuHPI GGTGA 2 cut(s) 47, 123
AvaI CYCGRG 1 cut(s) 125
BamHI GGATCC 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 116
BbvCI CCTCAGC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 203
BciT130I CCWGG 2 cut(s) 135, 180
BfaI CTAG 1 cut(s) 96
BisI GCNGC 1 cut(s) 217
BlsI GCNGC 1 cut(s) 218
Bme1390I CCNGG 2 cut(s) 135, 180
BmeT110I CYCGRG 1 cut(s) 125
BmiI GGNNCC 1 cut(s) 177
BmrFI CCNGG 2 cut(s) 135, 180
BpiI GAAGAC 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 185
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 1 cut(s) 190
BseBI CCWGG 2 cut(s) 135, 180
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 2 cut(s) 32, 185
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 1 cut(s) 190
BseRI GAGGAG 2 cut(s) 180, 183
BseXI GCAGC 1 cut(s) 203
BshFI GGCC 2 cut(s) 19, 193
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 1 cut(s) 233
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 32, 185
BsmFI GGGAC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 219
BsnI GGCC 2 cut(s) 19, 193
BsoBI CYCGRG 1 cut(s) 125
Bsp143I GATC 4 cut(s) 52, 81, 145, 175
BspANI GGCC 2 cut(s) 19, 193
BspCNI CTCAG 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 177
BspPI GGATC 4 cut(s) 47, 140, 170, 183
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 1 cut(s) 190
BssAI RCCGGY 1 cut(s) 232
BssMI GATC 4 cut(s) 52, 81, 145, 175
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 2 cut(s) 135, 180
Bst4CI ACNGT 1 cut(s) 79
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 17, 191
BstDEI CTNAG 1 cut(s) 66
BstF5I GGATG 1 cut(s) 106
BstKTI GATC 4 cut(s) 55, 84, 148, 178
BstMBI GATC 4 cut(s) 52, 81, 145, 175
BstNI CCWGG 2 cut(s) 135, 180
BstSCI CCNGG 2 cut(s) 133, 178
BstV1I GCAGC 1 cut(s) 203
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuRI GGCC 2 cut(s) 19, 193
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 2 cut(s) 17, 191
Cfr10I RCCGGY 1 cut(s) 232
CviAII CATG 1 cut(s) 161
CviJI RGCY 4 cut(s) 19, 65, 95, 193
CviKI_1 RGCY 4 cut(s) 19, 65, 95, 193
DdeI CTNAG 1 cut(s) 66
DpnI GATC 4 cut(s) 54, 83, 147, 177
DpnII GATC 4 cut(s) 52, 81, 145, 175
Eco147I AGGCCT 1 cut(s) 193
Eco88I CYCGRG 1 cut(s) 125
EcoRII CCWGG 2 cut(s) 133, 178
FaeI CATG 1 cut(s) 164
FaiI YATR 1 cut(s) 162
FaqI GGGAC 1 cut(s) 56
FatI CATG 1 cut(s) 160
Fnu4HI GCNGC 1 cut(s) 217
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 217
FspBI CTAG 1 cut(s) 96
GluI GCNGC 1 cut(s) 217
HaeIII GGCC 2 cut(s) 19, 193
HapII CCGG 1 cut(s) 233
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 1 cut(s) 164
HinfI GANTC 2 cut(s) 128, 239
HpaII CCGG 1 cut(s) 233
HphI GGTGA 2 cut(s) 47, 123
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 1 cut(s) 7
Hpy188III TCNNGA 1 cut(s) 243
Hpy8I GTNNAC 1 cut(s) 75
HpyAV CCTTC 1 cut(s) 204
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 1 cut(s) 219
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 1 cut(s) 164
Kzo9I GATC 4 cut(s) 52, 81, 145, 175
LpnPI CCDG 9 cut(s) 69, 99, 120, 142, 147, 165, 171, 175, 192
Lsp1109I GCAGC 1 cut(s) 203
MaeI CTAG 1 cut(s) 96
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 129
MalI GATC 4 cut(s) 54, 83, 147, 177
MboI GATC 4 cut(s) 52, 81, 145, 175
MboII GAAGA 2 cut(s) 118, 121
MflI RGATCY 1 cut(s) 175
MluCI AATT 1 cut(s) 29
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 3 cut(s) 61, 158, 161
MseI TTAA 1 cut(s) 87
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 2 cut(s) 135, 180
Mva1269I GAATGC 1 cut(s) 219
MvaI CCWGG 2 cut(s) 135, 180
NdeII GATC 4 cut(s) 52, 81, 145, 175
NlaIII CATG 1 cut(s) 164
NlaIV GGNNCC 1 cut(s) 177
NmuCI GTSAC 1 cut(s) 129
PceI AGGCCT 1 cut(s) 193
PctI GAATGC 1 cut(s) 219
PflMI CCANNNNNTGG 1 cut(s) 32
PkrI GCNGC 1 cut(s) 218
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
Psp6I CCWGG 2 cut(s) 133, 178
PspGI CCWGG 2 cut(s) 133, 178
PspN4I GGNNCC 1 cut(s) 177
PsuI RGATCY 1 cut(s) 175
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 1 cut(s) 217
Sau3AI GATC 4 cut(s) 52, 81, 145, 175
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 135, 180
SetI ASST 3 cut(s) 48, 118, 144
Sse9I AATT 1 cut(s) 29
SseBI AGGCCT 1 cut(s) 193
SspMI CTAG 1 cut(s) 96
StuI AGGCCT 1 cut(s) 193
StyD4I CCNGG 2 cut(s) 133, 178
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 1 cut(s) 48
TasI AATT 1 cut(s) 29
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 129
TseI GCWGC 1 cut(s) 216
Tsp45I GTSAC 1 cut(s) 129
TspDTI ATGAA 2 cut(s) 186, 212
TspRI CASTG 1 cut(s) 190
Van91I CCANNNNNTGG 1 cut(s) 32
XapI RAATTY 1 cut(s) 29
XspI CTAG 1 cut(s) 96
ZraI GACGTC 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.