MD03G1045800.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006212
Physical Location & Seq
Reverse (-)
3695047 .. 3699150
4104 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1045800.v1.1.491

Sequence Viewer

Length: 1629 bp
ATGGGTTCGTGTTGTTCGACTCAGAAAAGCTTGGGGCAGAGGAGTGATATGGTGGAGCAGAGCAAAGTGGACTACGCCGACGACGCTTCTCCGAAAGCCAAAGGCGGCTCGAAACGGTGGAAGAGGAAGAAGCCGGGAGCTGACAAAGCGGGTTGCGGAGATTTGGGCCATCAGATTGATATTCCGGGCAGATTGATCGGCAATGGAGGCAGCAAAGTCGCCTGCTTGTATACCCAGCAGGGAAAGAAAGGGACCAATCAGGACGCCATGCTTGTCTGGGAGAATTTCGCCACAAGAAGTGATACAACATTTTGTGGGGTGTTTGATGGCCACGGTCCGTTTGGTCACGTGGTCGCCAAGAAAGTTCGGGATTCTCTTCCTCTTATACTATGTACTCAGTGGAAAGCTAATTCAAACAGTGATCTGAGCAATCTCGATAAGGCTGAGAATGCGAATGGGAGCTCTAATTTGGAGGAACCTGCGTCGCCAAGCATTGATGATGAATGGTGCGAATCATTGGAGGTTCGGGAAAATGAAAAACTACCTGACATGTATCTCCCACTAAAAAAGTCATTCTTAAAGGCTTTCAAGTTAATGGACAAGGAACTAAAATTGCATCCAACCATCGATTGCTTCTGCAGTGGCACGACTGCTGTTACATTGGTAAAGCAGGGTCAGAATCTTGTAATTGGAAATGTTGGGGATTCAAGGGCTGTGCTAGCAACGAGAGACCAAGACAACTCTCTGATTGCTGTACAATTGACAGTAGATTTAAAGCCAGATCTTCCTGCGGAATCTGCTAGGATCCAGCAATGCAAGGGAAGGGTATTTGCTTTGCAGGATGAGCCAGAGGTTGCTCGTGTATGGTTGCCGAATAATGATTCTCCTGGTTTGGCAATGGCTAGAGCCTTTGGAGATTTCTGTCTGAAGGATTTTGGTTTAATTTCTGTGCCTGATGTTTTCTATCGCCATCTTACTGAAAGAGATGAGTTCATAATTCTTGCCACCGATGGGGTCTGGGACGTCCTTTCAAACAAGGAGGCTGTTGAAATTGTAGCTTCTGCCCCCGGTCATAAAACAGCAGCCCGGGCTCTAGTGGACTGTGCCGTTAGAGCCTGGAGGCTCAAATACCCTACTTCCAAGAATGATGATTGTGCCGTTGTATGCCTTTTTCTAGAACAGTTGTCTGAAGCTGGTGAGGCTGTGACAGAGAATGATATGATGAAGATTAATGAAGAGACGAAGGTTAATGAAATGATTAAGATTAATGACGAGACAAAGAGTAATAAAGAGACAAATATTAACGGAGAGGCAATGGAGAGGATGGCAATAACAGAAGAGAATACTGAGGATTCAGAAACTGGTAGTTCTCATGCCGTTGTTCTTGAGCATTCGGGTACCATACGAAGCTGCGATGAGATTGTGCCCGTATCTGATTCAACAGAACAAAAGCTTCCAACGAAGTTCGAGGGCCAGTCTAAAAGAAGTTTAGCAGAATGCATTTCAACAAAAGAGGACGAGGACTGGTCAGCCTTGGAAGGTATTACCCGCGTTAACAGTCTGTTAAGCATTCCCAGATTCTTATCCGGTGATAAAAGAGCCGCCAGTTGGAGAAAAAAGTGGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

543

Amino Acids

59.62

Weight (kDa)

5.24

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 187 - 370 1.5e-38 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1026
Acc36I ACCTGC 1 cut(s) 487
Acc65I GGTACC 1 cut(s) 1397
AccB1I GGYRCC 1 cut(s) 1397
AccI GTMKAC 1 cut(s) 230
AccII CGCG 1 cut(s) 1551
AciI CCGC 6 cut(s) 105, 149, 156, 791, 1549, 1602
AclWI GGATC 2 cut(s) 799, 812
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 1 cut(s) 283
AcuI CTGAAG 2 cut(s) 947, 1209
AcvI CACGTG 1 cut(s) 349
AcyI GRCGYC 2 cut(s) 264, 1023
AfaI GTAC 3 cut(s) 394, 756, 1399
AfiI CCNNNNNNNGG 2 cut(s) 1011, 1608
AflIII ACRYGT 1 cut(s) 549
AgsI TTSAA 7 cut(s) 414, 589, 708, 1032, 1049, 1440, 1506
AjnI CCWGG 2 cut(s) 886, 1115
AluBI AGCT 8 cut(s) 30, 140, 407, 462, 1058, 1193, 1410, 1453
AluI AGCT 8 cut(s) 30, 140, 407, 462, 1058, 1193, 1410, 1453
Alw21I GWGCWC 1 cut(s) 464
Alw26I GTCTC 4 cut(s) 723, 1232, 1268, 1286
AlwI GGATC 2 cut(s) 799, 812
AlwNI CAGNNNCTG 1 cut(s) 1361
Ama87I CYCGRG 1 cut(s) 1086
AoxI GGCC 3 cut(s) 166, 328, 1471
ApeKI GCWGC 3 cut(s) 210, 1082, 1410
ApoI RAATTY 1 cut(s) 283
AseI ATTAAT 2 cut(s) 1230, 1266
Asp718I GGTACC 1 cut(s) 1397
AspS9I GGNCC 4 cut(s) 166, 252, 335, 1471
AsuC2I CCSGG 5 cut(s) 135, 186, 1068, 1087, 1088
AsuHPI GGTGA 2 cut(s) 1208, 1601
AsuNHI GCTAGC 1 cut(s) 718
AvaI CYCGRG 1 cut(s) 1086
AvaII GGWCC 2 cut(s) 252, 335
BaeGI GKGCMC 1 cut(s) 1428
BalI TGGCCA 1 cut(s) 330
BamHI GGATCC 1 cut(s) 804
BanI GGYRCC 1 cut(s) 1397
BanII GRGCYC 2 cut(s) 464, 1093
BauI CACGAG 1 cut(s) 858
BbrPI CACGTG 1 cut(s) 349
Bbv12I GWGCWC 1 cut(s) 464
BbvI GCAGC 3 cut(s) 222, 1094, 1397
BccI CCATC 6 cut(s) 177, 320, 632, 978, 1004, 1318
BceAI ACGGC 3 cut(s) 1091, 1142, 1361
BcgI CGANNNNNNTGC 4 cut(s) 178, 199, 212, 233
BciT130I CCWGG 2 cut(s) 888, 1117
BcnI CCSGG 5 cut(s) 135, 186, 1068, 1087, 1088
BcoDI GTCTC 4 cut(s) 723, 1232, 1268, 1286
BfaI CTAG 5 cut(s) 719, 801, 903, 1094, 1175
BfmI CTRYAG 1 cut(s) 637
BfuAI ACCTGC 1 cut(s) 487
BglII AGATCT 1 cut(s) 781
BisI GCNGC 5 cut(s) 106, 211, 1083, 1411, 1602
BlsI GCNGC 5 cut(s) 107, 212, 1084, 1412, 1603
Bme1390I CCNGG 7 cut(s) 135, 186, 888, 1068, 1087, 1088, 1117
Bme18I GGWCC 2 cut(s) 252, 335
BmeT110I CYCGRG 1 cut(s) 1086
BmgT120I GGNCC 4 cut(s) 166, 252, 335, 1471
BmiI GGNNCC 4 cut(s) 253, 477, 806, 1399
BmrFI CCNGG 7 cut(s) 135, 186, 888, 1068, 1087, 1088, 1117
BmsI GCATC 1 cut(s) 625
BmtI GCTAGC 1 cut(s) 722
BpmI CTGGAG 1 cut(s) 1138
BpuEI CTTGAG 1 cut(s) 1406
BpuMI CCSGG 5 cut(s) 135, 186, 1068, 1087, 1088
Bsa29I ATCGAT 1 cut(s) 627
BsaAI YACGTR 1 cut(s) 349
BsaBI GATNNNNATC 1 cut(s) 1582
BsaHI GRCGYC 2 cut(s) 264, 1023
BsaI GGTCTC 1 cut(s) 723
BsaJI CCNNGG 4 cut(s) 331, 1066, 1086, 1533
BsaWI WCCGGW 1 cut(s) 1586
Bsc4I CCNNNNNNNGG 2 cut(s) 1011, 1608
Bse1I ACTGG 4 cut(s) 1366, 1474, 1529, 1605
Bse3DI GCAATG 4 cut(s) 208, 818, 903, 1320
Bse8I GATNNNNATC 1 cut(s) 1582
BseBI CCWGG 2 cut(s) 888, 1117
BseCI ATCGAT 1 cut(s) 627
BseDI CCNNGG 4 cut(s) 331, 1066, 1086, 1533
BseGI GGATG 3 cut(s) 616, 847, 1329
BseJI GATNNNNATC 1 cut(s) 1582
BseLI CCNNNNNNNGG 2 cut(s) 1011, 1608
BseMI GCAATG 4 cut(s) 208, 818, 903, 1320
BseMII CTCAG 5 cut(s) 35, 410, 416, 435, 1338
BseNI ACTGG 4 cut(s) 1366, 1474, 1529, 1605
BseRI GAGGAG 1 cut(s) 55
BseSI GKGCMC 1 cut(s) 1428
BseXI GCAGC 3 cut(s) 222, 1094, 1397
BseYI CCCAGC 1 cut(s) 234
Bsh1236I CGCG 1 cut(s) 1551
BshFI GGCC 3 cut(s) 168, 330, 1473
BshNI GGYRCC 1 cut(s) 1397
BshVI ATCGAT 1 cut(s) 627
BsiHKAI GWGCWC 1 cut(s) 464
BsiHKCI CYCGRG 1 cut(s) 1086
BsiSI CCGG 5 cut(s) 134, 185, 1068, 1087, 1587
BslFI GGGAC 2 cut(s) 265, 1034
BslI CCNNNNNNNGG 2 cut(s) 1011, 1608
BsmAI GTCTC 4 cut(s) 723, 1232, 1268, 1286
BsmBI CGTCTC 1 cut(s) 1232
BsmFI GGGAC 2 cut(s) 265, 1034
BsmI GAATGC 4 cut(s) 454, 1390, 1502, 1569
BsnI GGCC 3 cut(s) 168, 330, 1473
Bso31I GGTCTC 1 cut(s) 723
BsoBI CYCGRG 1 cut(s) 1086
Bsp1286I GDGCHC 3 cut(s) 464, 1093, 1428
Bsp1407I TGTACA 1 cut(s) 754
Bsp143I GATC 4 cut(s) 195, 421, 781, 804
BspACI CCGC 6 cut(s) 105, 149, 156, 791, 1549, 1602
BspANI GGCC 3 cut(s) 168, 330, 1473
BspCNI CTCAG 5 cut(s) 34, 409, 417, 436, 1339
BspDI ATCGAT 1 cut(s) 627
BspFNI CGCG 1 cut(s) 1551
BspLI GGNNCC 4 cut(s) 253, 477, 806, 1399
BspMAI CTGCAG 1 cut(s) 641
BspMI ACCTGC 1 cut(s) 487
BspOI GCTAGC 1 cut(s) 722
BspPI GGATC 2 cut(s) 799, 812
BspT107I GGYRCC 1 cut(s) 1397
BspTNI GGTCTC 1 cut(s) 723
BsrDI GCAATG 4 cut(s) 208, 818, 903, 1320
BsrGI TGTACA 1 cut(s) 754
BsrI ACTGG 4 cut(s) 1366, 1474, 1529, 1605
BssECI CCNNGG 4 cut(s) 331, 1066, 1086, 1533
BssMI GATC 4 cut(s) 195, 421, 781, 804
BssNAI GTATAC 1 cut(s) 231
BssNI GRCGYC 2 cut(s) 264, 1023
BssSI CACGAG 1 cut(s) 858
BssT1I CCWWGG 1 cut(s) 1533
Bst1107I GTATAC 1 cut(s) 231
Bst2BI CACGAG 1 cut(s) 858
Bst2UI CCWGG 2 cut(s) 888, 1117
Bst4CI ACNGT 7 cut(s) 117, 335, 419, 766, 1103, 1182, 1559
Bst6I CTCTTC 4 cut(s) 116, 381, 1230, 1332
BstACI GRCGYC 2 cut(s) 264, 1023
BstAUI TGTACA 1 cut(s) 754
BstBAI YACGTR 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 223, 720
BstDEI CTNAG 5 cut(s) 21, 396, 425, 444, 1347
BstDSI CCRYGG 1 cut(s) 331
BstF5I GGATG 3 cut(s) 616, 847, 1329
BstFNI CGCG 1 cut(s) 1551
BstKTI GATC 4 cut(s) 198, 424, 784, 807
BstMAI GTCTC 4 cut(s) 723, 1232, 1268, 1286
BstMBI GATC 4 cut(s) 195, 421, 781, 804
BstNI CCWGG 2 cut(s) 888, 1117
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 7 cut(s) 133, 184, 886, 1066, 1085, 1086, 1115
BstSFI CTRYAG 1 cut(s) 637
BstSLI GKGCMC 1 cut(s) 1428
BstUI CGCG 1 cut(s) 1551
BstV1I GCAGC 3 cut(s) 222, 1094, 1397
BstX2I RGATCY 2 cut(s) 781, 804
BstYI RGATCY 2 cut(s) 781, 804
BstZ17I GTATAC 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 627
BsuRI GGCC 3 cut(s) 168, 330, 1473
BsuTUI ATCGAT 1 cut(s) 627
BtgI CCRYGG 1 cut(s) 331
BtgZI GCGATG 1 cut(s) 1428
BtsCI GGATG 3 cut(s) 616, 847, 1329
BtsI GCAGTG 1 cut(s) 646
BtsIMutI CAGTG 3 cut(s) 404, 424, 646
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 2 cut(s) 223, 720
CaiI CAGNNNCTG 1 cut(s) 1361
Cfr13I GGNCC 4 cut(s) 166, 252, 335, 1471
Cfr9I CCCGGG 1 cut(s) 1086
ClaI ATCGAT 1 cut(s) 627
CpoI CGGWCCG 1 cut(s) 335
CseI GACGC 3 cut(s) 92, 272, 471
Csp6I GTAC 3 cut(s) 393, 755, 1398
CspI CGGWCCG 1 cut(s) 335
CviAII CATG 3 cut(s) 268, 550, 1373
CviQI GTAC 3 cut(s) 393, 755, 1398
DdeI CTNAG 5 cut(s) 21, 396, 425, 444, 1347
DpnI GATC 4 cut(s) 197, 423, 783, 806
DpnII GATC 4 cut(s) 195, 421, 781, 804
DraI TTTAAA 1 cut(s) 774
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 4 cut(s) 116, 381, 1230, 1332
EarI CTCTTC 4 cut(s) 116, 381, 1230, 1332
Ecl136II GAGCTC 1 cut(s) 462
Eco130I CCWWGG 1 cut(s) 1533
Eco24I GRGCYC 2 cut(s) 464, 1093
Eco31I GGTCTC 1 cut(s) 723
Eco47I GGWCC 2 cut(s) 252, 335
Eco53kI GAGCTC 1 cut(s) 462
Eco57I CTGAAG 2 cut(s) 947, 1209
Eco72I CACGTG 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 1086
EcoICRI GAGCTC 1 cut(s) 462
EcoRII CCWGG 2 cut(s) 886, 1115
EcoT14I CCWWGG 1 cut(s) 1533
EcoT22I ATGCAT 1 cut(s) 1502
EcoT38I GRGCYC 2 cut(s) 464, 1093
ErhI CCWWGG 1 cut(s) 1533
Esp3I CGTCTC 1 cut(s) 1232
FaeI CATG 3 cut(s) 271, 553, 1376
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 2 cut(s) 265, 1034
FatI CATG 3 cut(s) 267, 549, 1372
FauI CCCGC 2 cut(s) 142, 1556
FblI GTMKAC 1 cut(s) 230
Fnu4HI GCNGC 5 cut(s) 106, 211, 1083, 1411, 1602
FokI GGATG 3 cut(s) 603, 854, 1336
FriOI GRGCYC 2 cut(s) 464, 1093
Fsp4HI GCNGC 5 cut(s) 106, 211, 1083, 1411, 1602
FspBI CTAG 5 cut(s) 719, 801, 903, 1094, 1175
GluI GCNGC 5 cut(s) 106, 211, 1083, 1411, 1602
GsaI CCCAGC 1 cut(s) 238
GsuI CTGGAG 1 cut(s) 1138
HaeIII GGCC 3 cut(s) 168, 330, 1473
HapII CCGG 5 cut(s) 134, 185, 1068, 1087, 1587
HgaI GACGC 3 cut(s) 92, 272, 471
Hin1I GRCGYC 2 cut(s) 264, 1023
Hin1II CATG 3 cut(s) 271, 553, 1376
HincII GTYRAC 1 cut(s) 1555
HindII GTYRAC 1 cut(s) 1555
HindIII AAGCTT 2 cut(s) 28, 1451
HpaI GTTAAC 1 cut(s) 1555
HpaII CCGG 5 cut(s) 134, 185, 1068, 1087, 1587
HphI GGTGA 2 cut(s) 1208, 1601
Hpy166II GTNNAC 4 cut(s) 70, 231, 1099, 1555
Hpy188III TCNNGA 6 cut(s) 260, 368, 434, 527, 1175, 1385
Hpy8I GTNNAC 4 cut(s) 70, 231, 1099, 1555
Hpy99I CGWCG 3 cut(s) 83, 86, 487
HpyAV CCTTC 4 cut(s) 816, 922, 1237, 1532
HpyCH4III ACNGT 7 cut(s) 117, 335, 419, 766, 1103, 1182, 1559
HpyCH4IV ACGT 2 cut(s) 348, 1023
HpyCH4V TGCA 5 cut(s) 616, 639, 816, 838, 1500
HpyF3I CTNAG 5 cut(s) 21, 396, 425, 444, 1347
HpySE526I ACGT 2 cut(s) 348, 1023
Hsp92I GRCGYC 2 cut(s) 264, 1023
Hsp92II CATG 3 cut(s) 271, 553, 1376
KpnI GGTACC 1 cut(s) 1401
KspAI GTTAAC 1 cut(s) 1555
Kzo9I GATC 4 cut(s) 195, 421, 781, 804
LmnI GCTCC 3 cut(s) 55, 137, 459
Lsp1109I GCAGC 3 cut(s) 222, 1094, 1397
LweI GCATC 1 cut(s) 625
MaeI CTAG 5 cut(s) 719, 801, 903, 1094, 1175
MaeII ACGT 2 cut(s) 348, 1023
MaeIII GTNAC 3 cut(s) 344, 655, 1204
MalI GATC 4 cut(s) 197, 423, 783, 806
MboI GATC 4 cut(s) 195, 421, 781, 804
MboII GAAGA 7 cut(s) 133, 139, 368, 776, 1237, 1247, 1349
MfeI CAATTG 1 cut(s) 758
MflI RGATCY 2 cut(s) 781, 804
MhlI GDGCHC 3 cut(s) 464, 1093, 1428
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 9 cut(s) 283, 409, 466, 611, 687, 758, 942, 996, 1050
MluNI TGGCCA 1 cut(s) 330
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 3 cut(s) 644, 1481, 1589
Mox20I TGGCCA 1 cut(s) 330
Mph1103I ATGCAT 1 cut(s) 1502
MscI TGGCCA 1 cut(s) 330
Msp20I TGGCCA 1 cut(s) 330
MspI CCGG 5 cut(s) 134, 185, 1068, 1087, 1587
MspR9I CCNGG 7 cut(s) 135, 186, 888, 1068, 1087, 1088, 1117
MunI CAATTG 1 cut(s) 758
Mva1269I GAATGC 4 cut(s) 454, 1390, 1502, 1569
MvaI CCWGG 2 cut(s) 888, 1117
MvnI CGCG 1 cut(s) 1551
NciI CCSGG 5 cut(s) 135, 186, 1068, 1087, 1088
NdeII GATC 4 cut(s) 195, 421, 781, 804
NheI GCTAGC 1 cut(s) 718
NlaIII CATG 3 cut(s) 271, 553, 1376
NlaIV GGNNCC 4 cut(s) 253, 477, 806, 1399
NmuCI GTSAC 2 cut(s) 344, 1204
NsiI ATGCAT 1 cut(s) 1502
NspI RCATGY 1 cut(s) 553
PciI ACATGT 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 4 cut(s) 454, 1390, 1502, 1569
PfeI GAWTC 9 cut(s) 371, 512, 679, 704, 794, 881, 1352, 1436, 1578
PkrI GCNGC 5 cut(s) 107, 212, 1084, 1412, 1603
PleI GAGTC 1 cut(s) 13
PmaCI CACGTG 1 cut(s) 349
PmlI CACGTG 1 cut(s) 349
PpsI GAGTC 1 cut(s) 13
Ppu21I YACGTR 1 cut(s) 349
PscI ACATGT 1 cut(s) 549
PshBI ATTAAT 2 cut(s) 1230, 1266
Psp124BI GAGCTC 1 cut(s) 464
Psp6I CCWGG 2 cut(s) 886, 1115
PspCI CACGTG 1 cut(s) 349
PspFI CCCAGC 1 cut(s) 234
PspGI CCWGG 2 cut(s) 886, 1115
PspN4I GGNNCC 4 cut(s) 253, 477, 806, 1399
PspPI GGNCC 4 cut(s) 166, 252, 335, 1471
PstI CTGCAG 1 cut(s) 641
PstNI CAGNNNCTG 1 cut(s) 1361
PsuI RGATCY 2 cut(s) 781, 804
RsaI GTAC 3 cut(s) 394, 756, 1399
RsaNI GTAC 3 cut(s) 393, 755, 1398
Rsr2I CGGWCCG 1 cut(s) 335
RsrII CGGWCCG 1 cut(s) 335
SacI GAGCTC 1 cut(s) 464
SatI GCNGC 5 cut(s) 106, 211, 1083, 1411, 1602
Sau3AI GATC 4 cut(s) 195, 421, 781, 804
Sau96I GGNCC 4 cut(s) 166, 252, 335, 1471
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 7 cut(s) 135, 186, 888, 1068, 1087, 1088, 1117
SduI GDGCHC 3 cut(s) 464, 1093, 1428
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 1 cut(s) 637
SinI GGWCC 2 cut(s) 252, 335
SmaI CCCGGG 1 cut(s) 1088
SmlI CTYRAG 1 cut(s) 1385
SmoI CTYRAG 1 cut(s) 1385
SrfI GCCCGGGC 1 cut(s) 1088
Sse9I AATT 9 cut(s) 283, 409, 466, 611, 687, 758, 942, 996, 1050
SsiI CCGC 6 cut(s) 105, 149, 156, 791, 1549, 1602
SspI AATATT 1 cut(s) 1300
SspMI CTAG 5 cut(s) 719, 801, 903, 1094, 1175
SstI GAGCTC 1 cut(s) 464
StyD4I CCNGG 7 cut(s) 133, 184, 886, 1066, 1085, 1086, 1115
StyI CCWWGG 1 cut(s) 1533
TaaI ACNGT 7 cut(s) 117, 335, 419, 766, 1103, 1182, 1559
TaiI ACGT 2 cut(s) 351, 1026
TaqI TCGA 5 cut(s) 17, 110, 435, 627, 1467
TasI AATT 9 cut(s) 283, 409, 466, 611, 687, 758, 942, 996, 1050
TatI WGTACW 2 cut(s) 392, 754
TauI GCSGC 2 cut(s) 108, 1604
TfiI GAWTC 9 cut(s) 371, 512, 679, 704, 794, 881, 1352, 1436, 1578
TscAI CASTG 3 cut(s) 404, 424, 646
TseFI GTSAC 2 cut(s) 344, 1204
TseI GCWGC 3 cut(s) 210, 1082, 1410
Tsp45I GTSAC 2 cut(s) 344, 1204
TspDTI ATGAA 6 cut(s) 516, 549, 982, 1238, 1248, 1266
TspGWI ACGGA 2 cut(s) 327, 1320
TspMI CCCGGG 1 cut(s) 1086
TspRI CASTG 3 cut(s) 404, 424, 646
VpaK11BI GGWCC 2 cut(s) 252, 335
VspI ATTAAT 2 cut(s) 1230, 1266
XapI RAATTY 1 cut(s) 283
XbaI TCTAGA 1 cut(s) 1174
XceI RCATGY 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XmaI CCCGGG 1 cut(s) 1086
XmiI GTMKAC 1 cut(s) 230
XspI CTAG 5 cut(s) 719, 801, 903, 1094, 1175
ZraI GACGTC 1 cut(s) 1024
Zsp2I ATGCAT 1 cut(s) 1502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.