MD11G1048100.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006212
Physical Location & Seq
Reverse (-)
4184154 .. 4187472
3319 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1048100.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGGGTTCGTGTTGTTCGACGCAGAAAAGCTTGGGGCAGAGGATTGACATGGTGGAGGAGCAGAGCAAAGTGGATTACGCAGACGACGCTTCTCCGAAGGCCAGAGGCGGCTCGAAACGGTGGAAGAGGAAGAAGCCGAGAGGTGAGCAAGTGGATTGCAGAGACTTGGGCCATCAGATTGATATTCCGGGCAGATTGATCGGCAATGGAAGTAGCAAAGTCGCCTGCTTGTATACCCAGCAGGGAAAGAAAGGGACCAATCAGGACGCCATGCTTGTCTGGGAGAATTTCGCCACAAGAAGTGATACAACATTTTGTGGGGTGTTTGACGGGCATGGTCCATTTGGTCATATGGTTGCCAAGAAAGTTCGGGATTCTCTTCCTCTTATACTATGCACTCAGTGGAAAGCTAATTCAAGCGGTGACCTGAGCAATCTCAATAAGGCTGAGAATGCGAATGGGAGATCTAATTTTGAGGAACCTGTATCACCAAGCATTGATGATGAATGGTGCGAGTCGTTGGAGGTTAAGGAAAGTGAAAAACTACCTGAGATGTATCTACCACAAAAAAAGTCATTCTTAAAGGCTTTCAAGTTAATGGATAAGGAACTAAAGTTGCATCCAACAATCGATTGCTTCTGCAGTGGGACGACTGCGGTTACATTGGTAAAGCAGGGTCAGAATCTTGTGATTGGAAATGTTGGGGATTCAAGGGCTGTGCTAGCAACGAGAGACGATGACAACTCTCTGATTGCTGTACAGTTGACGGTAGACTTAAAGCCAGATCTTCCTGCGGAATCTGCGAGGATCCATCAATGCAAGGGAAGGGTGTTTGCTTTGCAGGATGAGCCAGAGGTTGCTCGTGTATGGTTGCCGAATAATGATTCTCCTGGTTTGGCAATGGCTAGGGCCTTTGGAGATTTCTGTCTAAAGGATTTTGGTTTAATTTCCGTCCCTGATGTTTGCTATCGCCATCTTACTGAAAGAGATGAGTTCATAATTCTTGCCACCGATGGGGTCTGGGACGTCCTTTCAAACAAGGAGGCTGTTGAAATTGTAGCTTCTGCCCCCGGTCATACAACGGCAGCCAGGGCTCTGGTAGAATGTGCCGTTAGAGCCTGGAGGCTCAAATACCCTACTTCCAAGAACGATGATTGTGCTGTTGTGTGCCTTTTTCTAGATCACTTGTCTGCAGCTGATGAGGCTGCGACAGAGAATGATATGACGAAGATGAATGAAGAGGCGAAGATTAATGAAGAGACGAAGGTTAATGAAGAGCTGAAGATTAATGGCGAGACAAAGAGTAACCACGAGACGAAGATTAACGGAGTGGCAATGGAAAGGATGGCAATCACAGATGTGAATATTGACGATTCAAAAACCGGTAGTTCTCATGCTGTTGTTCTTGAGCATTCGGGTACTGCAAGAAGCTCCGATGAGATCGAGTCTATATCTGATTCAACGGAACAAAAGCTTCCAACGAAGTTCGAGGGCCAGTCTAAAAGAAGTTTAGCAGAACGTATTTCAACAAAAGAGGACGAGGACTGGTCGGCGTTGGAAGGTGTTACCCGGGTTAATAGTCTGTTAAGCCTTCCCAGACTCTTATCTGGTGACAATTGGAGAAAAAAGTGGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

546

Amino Acids

60.24

Weight (kDa)

5.39

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 188 - 370 2.4e-38 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1029
AccI GTMKAC 2 cut(s) 233, 771
AciI CCGC 4 cut(s) 108, 420, 656, 794
AclWI GGATC 2 cut(s) 802, 815
AcsI RAATTY 1 cut(s) 286
AcuI CTGAAG 1 cut(s) 1301
AcyI GRCGYC 2 cut(s) 267, 1026
AdeI CACNNNGTG 1 cut(s) 402
AfaI GTAC 2 cut(s) 759, 1420
AfiI CCNNNNNNNGG 1 cut(s) 1014
AgeI ACCGGT 1 cut(s) 1382
AgsI TTSAA 8 cut(s) 417, 592, 711, 1035, 1052, 1377, 1461, 1527
AjnI CCWGG 3 cut(s) 889, 1088, 1118
AleI CACNNNNGTG 1 cut(s) 1358
AluBI AGCT 7 cut(s) 30, 410, 1061, 1196, 1279, 1431, 1474
AluI AGCT 7 cut(s) 30, 410, 1061, 1196, 1279, 1431, 1474
Alw26I GTCTC 5 cut(s) 156, 726, 1253, 1289, 1307
AlwI GGATC 2 cut(s) 802, 815
Ama87I CYCGRG 1 cut(s) 1569
AoxI GGCC 4 cut(s) 99, 169, 909, 1492
ApeKI GCWGC 3 cut(s) 1085, 1193, 1205
ApoI RAATTY 1 cut(s) 286
AseI ATTAAT 2 cut(s) 1251, 1287
AsiGI ACCGGT 1 cut(s) 1382
AspS9I GGNCC 5 cut(s) 169, 255, 338, 909, 1492
AsuC2I CCSGG 4 cut(s) 189, 1071, 1570, 1571
AsuHPI GGTGA 4 cut(s) 155, 434, 480, 1622
AsuNHI GCTAGC 1 cut(s) 721
AvaI CYCGRG 1 cut(s) 1569
AvaII GGWCC 2 cut(s) 255, 338
BamHI GGATCC 1 cut(s) 807
BanII GRGCYC 1 cut(s) 1096
BauI CACGAG 2 cut(s) 861, 1310
BbvI GCAGC 3 cut(s) 1097, 1192, 1205
BccI CCATC 5 cut(s) 180, 819, 981, 1007, 1339
BceAI ACGGC 2 cut(s) 1094, 1098
BcgI CGANNNNNNTGC 4 cut(s) 181, 215, 1139, 1173
BciT130I CCWGG 3 cut(s) 891, 1090, 1120
BcnI CCSGG 4 cut(s) 189, 1071, 1570, 1571
BcoDI GTCTC 5 cut(s) 156, 726, 1253, 1289, 1307
BfaI CTAG 3 cut(s) 722, 906, 1178
BfmI CTRYAG 2 cut(s) 640, 1191
BglII AGATCT 2 cut(s) 464, 784
BisI GCNGC 4 cut(s) 109, 1086, 1194, 1206
BlsI GCNGC 4 cut(s) 110, 1087, 1195, 1207
Bme1390I CCNGG 7 cut(s) 189, 891, 1071, 1090, 1120, 1570, 1571
Bme18I GGWCC 2 cut(s) 255, 338
BmeT110I CYCGRG 1 cut(s) 1569
BmgT120I GGNCC 5 cut(s) 169, 255, 338, 909, 1492
BmiI GGNNCC 3 cut(s) 256, 480, 809
BmrFI CCNGG 7 cut(s) 189, 891, 1071, 1090, 1120, 1570, 1571
BmsI GCATC 1 cut(s) 628
BmtI GCTAGC 1 cut(s) 725
BpmI CTGGAG 1 cut(s) 1141
Bpu10I CCTNAGC 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 1427
BpuMI CCSGG 4 cut(s) 189, 1071, 1570, 1571
Bsa29I ATCGAT 1 cut(s) 630
BsaBI GATNNNNATC 1 cut(s) 1349
BsaHI GRCGYC 2 cut(s) 267, 1026
BsaJI CCNNGG 3 cut(s) 1069, 1089, 1569
BsaWI WCCGGW 1 cut(s) 1382
Bsc4I CCNNNNNNNGG 1 cut(s) 1014
Bse118I RCCGGY 1 cut(s) 1382
Bse1I ACTGG 2 cut(s) 1495, 1550
Bse3DI GCAATG 3 cut(s) 211, 906, 1341
Bse8I GATNNNNATC 1 cut(s) 1349
BseBI CCWGG 3 cut(s) 891, 1090, 1120
BseCI ATCGAT 1 cut(s) 630
BseDI CCNNGG 3 cut(s) 1069, 1089, 1569
BseGI GGATG 3 cut(s) 619, 850, 1350
BseJI GATNNNNATC 1 cut(s) 1349
BseLI CCNNNNNNNGG 1 cut(s) 1014
BseMI GCAATG 3 cut(s) 211, 906, 1341
BseMII CTCAG 4 cut(s) 413, 419, 438, 540
BseNI ACTGG 2 cut(s) 1495, 1550
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 3 cut(s) 1097, 1192, 1205
BseYI CCCAGC 1 cut(s) 237
BshFI GGCC 4 cut(s) 101, 171, 911, 1494
BshTI ACCGGT 1 cut(s) 1382
BshVI ATCGAT 1 cut(s) 630
BsiHKCI CYCGRG 1 cut(s) 1569
BsiSI CCGG 4 cut(s) 188, 1071, 1383, 1570
BslFI GGGAC 4 cut(s) 268, 661, 938, 1037
BslI CCNNNNNNNGG 1 cut(s) 1014
BsmAI GTCTC 5 cut(s) 156, 726, 1253, 1289, 1307
BsmBI CGTCTC 3 cut(s) 726, 1253, 1307
BsmFI GGGAC 4 cut(s) 268, 661, 938, 1037
BsmI GAATGC 2 cut(s) 457, 1411
BsnI GGCC 4 cut(s) 101, 171, 911, 1494
BsoBI CYCGRG 1 cut(s) 1569
Bsp1286I GDGCHC 1 cut(s) 1096
Bsp1407I TGTACA 1 cut(s) 757
Bsp143I GATC 6 cut(s) 198, 464, 784, 807, 1180, 1440
BspACI CCGC 4 cut(s) 108, 420, 656, 794
BspANI GGCC 4 cut(s) 101, 171, 911, 1494
BspCNI CTCAG 4 cut(s) 412, 420, 439, 541
BspDI ATCGAT 1 cut(s) 630
BspLI GGNNCC 3 cut(s) 256, 480, 809
BspMAI CTGCAG 2 cut(s) 644, 1195
BspOI GCTAGC 1 cut(s) 725
BspPI GGATC 2 cut(s) 802, 815
BspQI GCTCTTC 1 cut(s) 1269
BsrDI GCAATG 3 cut(s) 211, 906, 1341
BsrFI RCCGGY 1 cut(s) 1382
BsrGI TGTACA 1 cut(s) 757
BsrI ACTGG 2 cut(s) 1495, 1550
BssAI RCCGGY 1 cut(s) 1382
BssECI CCNNGG 3 cut(s) 1069, 1089, 1569
BssMI GATC 6 cut(s) 198, 464, 784, 807, 1180, 1440
BssNAI GTATAC 1 cut(s) 234
BssNI GRCGYC 2 cut(s) 267, 1026
BssSI CACGAG 2 cut(s) 861, 1310
Bst1107I GTATAC 1 cut(s) 234
Bst2BI CACGAG 2 cut(s) 861, 1310
Bst2UI CCWGG 3 cut(s) 891, 1090, 1120
Bst4CI ACNGT 3 cut(s) 120, 762, 769
Bst6I CTCTTC 5 cut(s) 119, 384, 1233, 1251, 1269
BstACI GRCGYC 2 cut(s) 267, 1026
BstAUI TGTACA 1 cut(s) 757
BstC8I GCNNGC 2 cut(s) 226, 723
BstDEI CTNAG 4 cut(s) 399, 428, 447, 549
BstEII GGTNACC 1 cut(s) 422
BstF5I GGATG 3 cut(s) 619, 850, 1350
BstKTI GATC 6 cut(s) 201, 467, 787, 810, 1183, 1443
BstMAI GTCTC 5 cut(s) 156, 726, 1253, 1289, 1307
BstMBI GATC 6 cut(s) 198, 464, 784, 807, 1180, 1440
BstMWI GCNNNNNNNGC 8 cut(s) 86, 452, 722, 800, 847, 1091, 1115, 1202
BstNI CCWGG 3 cut(s) 891, 1090, 1120
BstPI GGTNACC 1 cut(s) 422
BstSCI CCNGG 7 cut(s) 187, 889, 1069, 1088, 1118, 1568, 1569
BstSFI CTRYAG 2 cut(s) 640, 1191
BstV1I GCAGC 3 cut(s) 1097, 1192, 1205
BstX2I RGATCY 3 cut(s) 464, 784, 807
BstXI CCANNNNNNTGG 1 cut(s) 1096
BstYI RGATCY 3 cut(s) 464, 784, 807
BstZ17I GTATAC 1 cut(s) 234
Bsu15I ATCGAT 1 cut(s) 630
BsuRI GGCC 4 cut(s) 101, 171, 911, 1494
BsuTUI ATCGAT 1 cut(s) 630
BtsCI GGATG 3 cut(s) 619, 850, 1350
BtsI GCAGTG 1 cut(s) 649
BtsIMutI CAGTG 2 cut(s) 407, 649
Cac8I GCNNGC 2 cut(s) 226, 723
Cfr10I RCCGGY 1 cut(s) 1382
Cfr13I GGNCC 5 cut(s) 169, 255, 338, 909, 1492
Cfr9I CCCGGG 1 cut(s) 1569
ClaI ATCGAT 1 cut(s) 630
CseI GACGC 3 cut(s) 28, 95, 275
Csp6I GTAC 2 cut(s) 758, 1419
CspAI ACCGGT 1 cut(s) 1382
CviAII CATG 4 cut(s) 49, 271, 335, 1394
CviQI GTAC 2 cut(s) 758, 1419
DdeI CTNAG 4 cut(s) 399, 428, 447, 549
DpnI GATC 6 cut(s) 200, 466, 786, 809, 1182, 1442
DpnII GATC 6 cut(s) 198, 464, 784, 807, 1180, 1440
DraIII CACNNNGTG 1 cut(s) 402
Eam1104I CTCTTC 5 cut(s) 119, 384, 1233, 1251, 1269
EarI CTCTTC 5 cut(s) 119, 384, 1233, 1251, 1269
Eco24I GRGCYC 1 cut(s) 1096
Eco47I GGWCC 2 cut(s) 255, 338
Eco57I CTGAAG 1 cut(s) 1301
Eco88I CYCGRG 1 cut(s) 1569
Eco91I GGTNACC 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 909
EcoO65I GGTNACC 1 cut(s) 422
EcoRII CCWGG 3 cut(s) 889, 1088, 1118
EcoT38I GRGCYC 1 cut(s) 1096
Esp3I CGTCTC 3 cut(s) 726, 1253, 1307
FaeI CATG 4 cut(s) 52, 274, 338, 1397
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 4 cut(s) 268, 661, 938, 1037
FatI CATG 4 cut(s) 48, 270, 334, 1393
FauNDI CATATG 1 cut(s) 351
FblI GTMKAC 2 cut(s) 233, 771
Fnu4HI GCNGC 4 cut(s) 109, 1086, 1194, 1206
FokI GGATG 3 cut(s) 606, 857, 1357
FriOI GRGCYC 1 cut(s) 1096
Fsp4HI GCNGC 4 cut(s) 109, 1086, 1194, 1206
FspBI CTAG 3 cut(s) 722, 906, 1178
GluI GCNGC 4 cut(s) 109, 1086, 1194, 1206
GsaI CCCAGC 1 cut(s) 241
GsuI CTGGAG 1 cut(s) 1141
HaeIII GGCC 4 cut(s) 101, 171, 911, 1494
HapII CCGG 4 cut(s) 188, 1071, 1383, 1570
HgaI GACGC 3 cut(s) 28, 95, 275
Hin1I GRCGYC 2 cut(s) 267, 1026
Hin1II CATG 4 cut(s) 52, 274, 338, 1397
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 2 cut(s) 28, 1472
HpaII CCGG 4 cut(s) 188, 1071, 1383, 1570
HphI GGTGA 4 cut(s) 155, 434, 480, 1622
Hpy166II GTNNAC 3 cut(s) 234, 765, 772
Hpy188I TCNGA 6 cut(s) 96, 177, 681, 750, 1435, 1456
Hpy188III TCNNGA 4 cut(s) 263, 371, 1178, 1406
Hpy8I GTNNAC 3 cut(s) 234, 765, 772
Hpy99I CGWCG 2 cut(s) 22, 89
HpyAV CCTTC 5 cut(s) 91, 819, 1258, 1553, 1601
HpyCH4III ACNGT 3 cut(s) 120, 762, 769
HpyCH4IV ACGT 2 cut(s) 1026, 1519
HpyCH4V TGCA 8 cut(s) 159, 396, 619, 642, 819, 841, 1193, 1424
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 452, 722, 800, 847, 1091, 1115, 1202
HpyF3I CTNAG 4 cut(s) 399, 428, 447, 549
HpySE526I ACGT 2 cut(s) 1026, 1519
Hsp92I GRCGYC 2 cut(s) 267, 1026
Hsp92II CATG 4 cut(s) 52, 274, 338, 1397
Kzo9I GATC 6 cut(s) 198, 464, 784, 807, 1180, 1440
LguI GCTCTTC 1 cut(s) 1269
LmnI GCTCC 2 cut(s) 58, 1436
Lsp1109I GCAGC 3 cut(s) 1097, 1192, 1205
LweI GCATC 1 cut(s) 628
MaeI CTAG 3 cut(s) 722, 906, 1178
MaeII ACGT 2 cut(s) 1026, 1519
MaeIII GTNAC 5 cut(s) 422, 658, 1304, 1564, 1610
MalI GATC 6 cut(s) 200, 466, 786, 809, 1182, 1442
MboI GATC 6 cut(s) 198, 464, 784, 807, 1180, 1440
MfeI CAATTG 1 cut(s) 1615
MflI RGATCY 3 cut(s) 464, 784, 807
MhlI GDGCHC 1 cut(s) 1096
MluCI AATT 7 cut(s) 286, 412, 469, 945, 999, 1053, 1615
MlyI GAGTC 3 cut(s) 524, 1454, 1593
MmeI TCCRAC 4 cut(s) 501, 647, 1502, 1536
MslI CAYNNNNRTG 1 cut(s) 1358
MspA1I CMGCKG 1 cut(s) 1196
MspI CCGG 4 cut(s) 188, 1071, 1383, 1570
MspR9I CCNGG 7 cut(s) 189, 891, 1071, 1090, 1120, 1570, 1571
MunI CAATTG 1 cut(s) 1615
Mva1269I GAATGC 2 cut(s) 457, 1411
MvaI CCWGG 3 cut(s) 891, 1090, 1120
MwoI GCNNNNNNNGC 8 cut(s) 86, 452, 722, 800, 847, 1091, 1115, 1202
NciI CCSGG 4 cut(s) 189, 1071, 1570, 1571
NdeI CATATG 1 cut(s) 351
NdeII GATC 6 cut(s) 198, 464, 784, 807, 1180, 1440
NheI GCTAGC 1 cut(s) 721
NlaIII CATG 4 cut(s) 52, 274, 338, 1397
NlaIV GGNNCC 3 cut(s) 256, 480, 809
NmeAIII GCCGAG 1 cut(s) 162
NmuCI GTSAC 2 cut(s) 422, 1610
OliI CACNNNNGTG 1 cut(s) 1358
PciSI GCTCTTC 1 cut(s) 1269
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 2 cut(s) 457, 1411
PfeI GAWTC 7 cut(s) 374, 682, 707, 797, 884, 1373, 1457
PinAI ACCGGT 1 cut(s) 1382
PkrI GCNGC 4 cut(s) 110, 1087, 1195, 1207
PleI GAGTC 3 cut(s) 523, 1453, 1593
PpsI GAGTC 3 cut(s) 523, 1453, 1593
PshBI ATTAAT 2 cut(s) 1251, 1287
Psp6I CCWGG 3 cut(s) 889, 1088, 1118
PspEI GGTNACC 1 cut(s) 422
PspFI CCCAGC 1 cut(s) 237
PspGI CCWGG 3 cut(s) 889, 1088, 1118
PspN4I GGNNCC 3 cut(s) 256, 480, 809
PspPI GGNCC 5 cut(s) 169, 255, 338, 909, 1492
PstI CTGCAG 2 cut(s) 644, 1195
PsuI RGATCY 3 cut(s) 464, 784, 807
PvuII CAGCTG 1 cut(s) 1196
RsaI GTAC 2 cut(s) 759, 1420
RsaNI GTAC 2 cut(s) 758, 1419
RseI CAYNNNNRTG 1 cut(s) 1358
SapI GCTCTTC 1 cut(s) 1269
SatI GCNGC 4 cut(s) 109, 1086, 1194, 1206
Sau3AI GATC 6 cut(s) 198, 464, 784, 807, 1180, 1440
Sau96I GGNCC 5 cut(s) 169, 255, 338, 909, 1492
SchI GAGTC 3 cut(s) 524, 1454, 1593
ScrFI CCNGG 7 cut(s) 189, 891, 1071, 1090, 1120, 1570, 1571
SduI GDGCHC 1 cut(s) 1096
SfaNI GCATC 1 cut(s) 628
SfcI CTRYAG 2 cut(s) 640, 1191
SinI GGWCC 2 cut(s) 255, 338
SmaI CCCGGG 1 cut(s) 1571
SmiMI CAYNNNNRTG 1 cut(s) 1358
SmlI CTYRAG 1 cut(s) 1406
SmoI CTYRAG 1 cut(s) 1406
Sse9I AATT 7 cut(s) 286, 412, 469, 945, 999, 1053, 1615
SsiI CCGC 4 cut(s) 108, 420, 656, 794
SspI AATATT 1 cut(s) 1366
SspMI CTAG 3 cut(s) 722, 906, 1178
StyD4I CCNGG 7 cut(s) 187, 889, 1069, 1088, 1118, 1568, 1569
TaaI ACNGT 3 cut(s) 120, 762, 769
TaiI ACGT 2 cut(s) 1029, 1522
TaqI TCGA 5 cut(s) 17, 113, 630, 1443, 1488
TasI AATT 7 cut(s) 286, 412, 469, 945, 999, 1053, 1615
TatI WGTACW 1 cut(s) 757
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 7 cut(s) 374, 682, 707, 797, 884, 1373, 1457
TscAI CASTG 2 cut(s) 407, 649
TseFI GTSAC 2 cut(s) 422, 1610
TseI GCWGC 3 cut(s) 1085, 1193, 1205
Tsp45I GTSAC 2 cut(s) 422, 1610
TspDTI ATGAA 6 cut(s) 519, 985, 1247, 1251, 1269, 1287
TspGWI ACGGA 3 cut(s) 940, 1341, 1478
TspMI CCCGGG 1 cut(s) 1569
TspRI CASTG 2 cut(s) 407, 649
VpaK11BI GGWCC 2 cut(s) 255, 338
VspI ATTAAT 2 cut(s) 1251, 1287
XapI RAATTY 1 cut(s) 286
XbaI TCTAGA 1 cut(s) 1177
XmaI CCCGGG 1 cut(s) 1569
XmiI GTMKAC 2 cut(s) 233, 771
XspI CTAG 3 cut(s) 722, 906, 1178
ZraI GACGTC 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.