Rh5DG519600

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006212
Physical Location & Seq
Forward (+)
80673363 .. 80678531
5169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG519600.1

Sequence Viewer

Length: 1254 bp
ATGGTTGCCAAGAAAGTTCGGGATTCTCTTCCGCTCTTACTATGCACTCAGTGGAAAGCTAATTCAGATGGTGACCTGAGCAACCTCAGTAAGACTGAAAATGCACATGGAAATTCTAATTTTGAAGAACCGCCATCTCCAAGCATTGATGATGAATGGCGCGAGTCAATGGAGGTCCAGGAAAGTGAAAATCTCCCCGACATGTATGCACCACTAAAAAAGTCATTCTTAAAGGCTTTCAAGTTAATGGATAAGGAATTAAGATTGCATCCCACAATCGATTGCTTCTGCAGTGGGACGACTGCTGTTACATTGATAAAGCAGGGTCAGAATCTTGTACTTGGAAATGTTGGGGATTCGAGAGCTGTGCTGGCAACAAGAGACAACGAGAACTCTTTGATTGCTGTACAATTGACAGTAGACTTAAAGCCTGATCTTCCTAGGGAAGCTGCTAGGATTCACCAATGCAAAGGAAGGGTATTTGCATTGCAGGATGAGCCAGAGGTTGCTCGTGTTTGGTTACCTAATAATGATTCTCCTGGTTTAGCAATGGCTAGAGCCTTTGGAGACTTCTGTCTAAAGGATTTTGGTTTAATTTCTGTCCCAGATGTTTACTATCGCCACCTAACGGAAAAAGATGAATTCATTATACTTGCCACCGATGGGGTTTGGGATGTCCTTTCAAACAAGGAAGCAATCGAAATTGTGGCTTCTGCCCCTAGTCACACAACAGCAGCTAGGGCTCTTGTAGACTGTGCCGTTAGAGCTTGGAGGCTTAAATACCCTACATCCAAGAATGATGATTGTGGTGTTGTATGCCTCTTTTTAGAACAGTTTTCTGTAGCTGATGAGGTTGTGACAGATACTGAAGTGACGAAGAATAACGGAGAGGAAATGGAGATGATGGCAACTGCACAAGGTAACAGTCAGGATGTAGAGATCGTTGATTCTCATGTCATTGCTGTTGAGCATTTGGGTAGTGATTTAGAACTCAGTGATTCTCATGCCACTGGTCTTGAGCATTCCAGTACTGTATGGAGCTCCGATGAGATTGTTCCTGTGTCCGAGTCAACAGAGAAAAAGCTTCCTATGAAGTTAGAGGGCCAGTCTAAAAGAAGTCTGGCAGAGTGCATTTCAACAGATGAAGATTGGTCAGCATTGGAAGGCGTTACCCGGGTTAATAGTTTGTTAAGTCTTCCGAGATTGTTATCTGGTGACAAAAGATCTCCCAGTTGGAGAAAGAAGTGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

417

Amino Acids

46.2

Weight (kDa)

4.84

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 20 - 254 1.4e-40 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 34
AccI GTMKAC 2 cut(s) 420, 750
AccII CGCG 1 cut(s) 162
AciI CCGC 2 cut(s) 32, 131
AcsI RAATTY 2 cut(s) 112, 641
AcuI CTGAAG 1 cut(s) 888
AdeI CACNNNGTG 1 cut(s) 51
AfaI GTAC 3 cut(s) 339, 408, 1030
AfiI CCNNNNNNNGG 2 cut(s) 628, 663
AflIII ACRYGT 1 cut(s) 201
AgsI TTSAA 4 cut(s) 125, 241, 684, 1137
AjnI CCWGG 2 cut(s) 177, 538
AluBI AGCT 8 cut(s) 59, 365, 449, 737, 767, 845, 1041, 1084
AluI AGCT 8 cut(s) 59, 365, 449, 737, 767, 845, 1041, 1084
Alw21I GWGCWC 1 cut(s) 1043
Alw26I GTCTC 2 cut(s) 375, 561
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 1 cut(s) 1173
AoxI GGCC 1 cut(s) 1102
ApeKI GCWGC 2 cut(s) 449, 734
ApoI RAATTY 2 cut(s) 112, 641
AspA2I CCTAGG 1 cut(s) 440
AspLEI GCGC 1 cut(s) 162
AspS9I GGNCC 2 cut(s) 175, 1102
AsuC2I CCSGG 2 cut(s) 1174, 1175
AsuHPI GGTGA 3 cut(s) 83, 452, 1226
AvaI CYCGRG 1 cut(s) 1173
AvaII GGWCC 1 cut(s) 175
AvrII CCTAGG 1 cut(s) 440
BaeI ACNNNNGTAYC 2 cut(s) 855, 888
BanII GRGCYC 2 cut(s) 745, 1043
BauI CACGAG 1 cut(s) 510
BbsI GAAGAC 1 cut(s) 1187
Bbv12I GWGCWC 1 cut(s) 1043
BbvI GCAGC 2 cut(s) 436, 746
BccI CCATC 4 cut(s) 62, 142, 656, 898
BceAI ACGGC 1 cut(s) 743
BcgI CGANNNNNNTGC 4 cut(s) 188, 222, 349, 383
BciT130I CCWGG 2 cut(s) 179, 540
BcnI CCSGG 2 cut(s) 1174, 1175
BcoDI GTCTC 2 cut(s) 375, 561
BfaI CTAG 5 cut(s) 441, 453, 555, 720, 738
BfmI CTRYAG 2 cut(s) 289, 840
BglII AGATCT 1 cut(s) 1223
BisI GCNGC 2 cut(s) 450, 735
BlnI CCTAGG 1 cut(s) 440
BlsI GCNGC 2 cut(s) 451, 736
BmcAI AGTACT 1 cut(s) 1030
Bme1390I CCNGG 4 cut(s) 179, 540, 1174, 1175
Bme18I GGWCC 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 1173
BmgT120I GGNCC 2 cut(s) 175, 1102
BmrFI CCNGG 4 cut(s) 179, 540, 1174, 1175
BmrI ACTGGG 1 cut(s) 1224
BmsI GCATC 1 cut(s) 277
BmuI ACTGGG 1 cut(s) 1224
BoxI GACNNNNGTC 1 cut(s) 573
BpiI GAAGAC 1 cut(s) 1187
Bpu10I CCTNAGC 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 1037
BpuMI CCSGG 2 cut(s) 1174, 1175
Bsa29I ATCGAT 1 cut(s) 279
BsaBI GATNNNNATC 1 cut(s) 1207
BsaJI CCNNGG 2 cut(s) 440, 1173
Bsc4I CCNNNNNNNGG 2 cut(s) 628, 663
Bse1I ACTGG 4 cut(s) 1015, 1026, 1105, 1230
Bse3DI GCAATG 3 cut(s) 485, 555, 957
Bse8I GATNNNNATC 1 cut(s) 1207
BseBI CCWGG 2 cut(s) 179, 540
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 440, 1173
BseGI GGATG 5 cut(s) 268, 499, 679, 788, 937
BseJI GATNNNNATC 1 cut(s) 1207
BseLI CCNNNNNNNGG 2 cut(s) 628, 663
BseMI GCAATG 3 cut(s) 485, 555, 957
BseMII CTCAG 4 cut(s) 62, 68, 100, 1006
BseNI ACTGG 4 cut(s) 1015, 1026, 1105, 1230
BseXI GCAGC 2 cut(s) 436, 746
BsgI GTGCAG 1 cut(s) 897
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 1 cut(s) 1104
BshVI ATCGAT 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 1043
BsiHKCI CYCGRG 1 cut(s) 1173
BsiSI CCGG 1 cut(s) 1174
BslFI GGGAC 2 cut(s) 310, 587
BslI CCNNNNNNNGG 2 cut(s) 628, 663
BsmAI GTCTC 2 cut(s) 375, 561
BsmFI GGGAC 2 cut(s) 310, 587
BsmI GAATGC 1 cut(s) 1021
BsnI GGCC 1 cut(s) 1104
BsoBI CYCGRG 1 cut(s) 1173
Bsp1286I GDGCHC 2 cut(s) 745, 1043
Bsp1407I TGTACA 1 cut(s) 406
Bsp143I GATC 3 cut(s) 433, 939, 1223
BspACI CCGC 2 cut(s) 32, 131
BspANI GGCC 1 cut(s) 1104
BspCNI CTCAG 4 cut(s) 61, 69, 99, 1005
BspDI ATCGAT 1 cut(s) 279
BspFNI CGCG 1 cut(s) 162
BspMAI CTGCAG 1 cut(s) 293
BsrBI CCGCTC 1 cut(s) 34
BsrDI GCAATG 3 cut(s) 485, 555, 957
BsrGI TGTACA 1 cut(s) 406
BsrI ACTGG 4 cut(s) 1015, 1026, 1105, 1230
BssECI CCNNGG 2 cut(s) 440, 1173
BssMI GATC 3 cut(s) 433, 939, 1223
BssSI CACGAG 1 cut(s) 510
BssT1I CCWWGG 1 cut(s) 440
Bst2BI CACGAG 1 cut(s) 510
Bst2UI CCWGG 2 cut(s) 179, 540
Bst4CI ACNGT 5 cut(s) 418, 755, 834, 926, 1033
Bst6I CTCTTC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 406
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 4 cut(s) 48, 77, 86, 992
BstEII GGTNACC 2 cut(s) 71, 519
BstF5I GGATG 5 cut(s) 268, 499, 679, 788, 937
BstFNI CGCG 1 cut(s) 162
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 3 cut(s) 436, 942, 1226
BstMAI GTCTC 2 cut(s) 375, 561
BstMBI GATC 3 cut(s) 433, 939, 1223
BstMWI GCNNNNNNNGC 4 cut(s) 371, 496, 740, 764
BstNI CCWGG 2 cut(s) 179, 540
BstNSI RCATGY 1 cut(s) 205
BstPAI GACNNNNGTC 1 cut(s) 573
BstPI GGTNACC 2 cut(s) 71, 519
BstSCI CCNGG 4 cut(s) 177, 538, 1172, 1173
BstSFI CTRYAG 2 cut(s) 289, 840
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 2 cut(s) 436, 746
BstV2I GAAGAC 1 cut(s) 1187
BstX2I RGATCY 1 cut(s) 1223
BstYI RGATCY 1 cut(s) 1223
Bsu15I ATCGAT 1 cut(s) 279
BsuRI GGCC 1 cut(s) 1104
BsuTUI ATCGAT 1 cut(s) 279
BtsCI GGATG 5 cut(s) 268, 499, 679, 788, 937
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 4 cut(s) 56, 298, 1000, 1008
Cac8I GCNNGC 1 cut(s) 372
CaiI CAGNNNCTG 1 cut(s) 866
CfoI GCGC 1 cut(s) 162
Cfr13I GGNCC 2 cut(s) 175, 1102
Cfr9I CCCGGG 1 cut(s) 1173
ClaI ATCGAT 1 cut(s) 279
Csp6I GTAC 3 cut(s) 338, 407, 1029
CspCI CAANNNNNGTGG 2 cut(s) 997, 1032
CviAII CATG 4 cut(s) 107, 202, 953, 1004
CviQI GTAC 3 cut(s) 338, 407, 1029
DdeI CTNAG 4 cut(s) 48, 77, 86, 992
DpnI GATC 3 cut(s) 435, 941, 1225
DpnII GATC 3 cut(s) 433, 939, 1223
DraIII CACNNNGTG 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 1041
Eco130I CCWWGG 1 cut(s) 440
Eco24I GRGCYC 2 cut(s) 745, 1043
Eco47I GGWCC 1 cut(s) 175
Eco53kI GAGCTC 1 cut(s) 1041
Eco57I CTGAAG 1 cut(s) 888
Eco88I CYCGRG 1 cut(s) 1173
Eco91I GGTNACC 2 cut(s) 71, 519
EcoICRI GAGCTC 1 cut(s) 1041
EcoO65I GGTNACC 2 cut(s) 71, 519
EcoRI GAATTC 1 cut(s) 641
EcoRII CCWGG 2 cut(s) 177, 538
EcoT14I CCWWGG 1 cut(s) 440
EcoT38I GRGCYC 2 cut(s) 745, 1043
ErhI CCWWGG 1 cut(s) 440
FaeI CATG 4 cut(s) 110, 205, 956, 1007
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FaqI GGGAC 2 cut(s) 310, 587
FatI CATG 4 cut(s) 106, 201, 952, 1003
FblI GTMKAC 2 cut(s) 420, 750
Fnu4HI GCNGC 2 cut(s) 450, 735
FokI GGATG 5 cut(s) 255, 506, 686, 775, 944
FriOI GRGCYC 2 cut(s) 745, 1043
Fsp4HI GCNGC 2 cut(s) 450, 735
FspBI CTAG 5 cut(s) 441, 453, 555, 720, 738
GlaI GCGC 1 cut(s) 161
GluI GCNGC 2 cut(s) 450, 735
HaeIII GGCC 1 cut(s) 1104
HapII CCGG 1 cut(s) 1174
HhaI GCGC 1 cut(s) 162
Hin1II CATG 4 cut(s) 110, 205, 956, 1007
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HincII GTYRAC 1 cut(s) 1071
HindII GTYRAC 1 cut(s) 1071
HindIII AAGCTT 1 cut(s) 1082
HinfI GANTC 9 cut(s) 23, 164, 331, 356, 457, 533, 947, 998, 1067
HpaII CCGG 1 cut(s) 1174
HphI GGTGA 3 cut(s) 83, 452, 1226
Hpy166II GTNNAC 4 cut(s) 421, 613, 751, 1071
Hpy188I TCNGA 5 cut(s) 67, 330, 1045, 1066, 1200
Hpy188III TCNNGA 4 cut(s) 20, 360, 929, 1016
Hpy8I GTNNAC 4 cut(s) 421, 613, 751, 1071
HpyAV CCTTC 2 cut(s) 468, 1157
HpyCH4III ACNGT 5 cut(s) 418, 755, 834, 926, 1033
HpyF10VI GCNNNNNNNGC 4 cut(s) 371, 496, 740, 764
HpyF3I CTNAG 4 cut(s) 48, 77, 86, 992
Hsp92II CATG 4 cut(s) 110, 205, 956, 1007
HspAI GCGC 1 cut(s) 160
Kzo9I GATC 3 cut(s) 433, 939, 1223
LmnI GCTCC 2 cut(s) 1038, 1046
Lsp1109I GCAGC 2 cut(s) 436, 746
LweI GCATC 1 cut(s) 277
MaeI CTAG 5 cut(s) 441, 453, 555, 720, 738
MaeIII GTNAC 9 cut(s) 71, 307, 519, 722, 856, 871, 920, 1168, 1214
MalI GATC 3 cut(s) 435, 941, 1225
MbiI CCGCTC 1 cut(s) 34
MboI GATC 3 cut(s) 433, 939, 1223
MboII GAAGA 6 cut(s) 20, 137, 428, 889, 1157, 1187
MfeI CAATTG 1 cut(s) 410
MflI RGATCY 1 cut(s) 1223
MhlI GDGCHC 2 cut(s) 745, 1043
MluCI AATT 8 cut(s) 61, 112, 118, 257, 410, 594, 641, 702
MlyI GAGTC 2 cut(s) 173, 1076
MmeI TCCRAC 1 cut(s) 1214
MnlI CCTC 8 cut(s) 95, 166, 496, 765, 830, 844, 883, 1093
MseI TTAA 8 cut(s) 230, 245, 260, 425, 593, 777, 1179, 1190
MspI CCGG 1 cut(s) 1174
MspR9I CCNGG 4 cut(s) 179, 540, 1174, 1175
MunI CAATTG 1 cut(s) 410
Mva1269I GAATGC 1 cut(s) 1021
MvaI CCWGG 2 cut(s) 179, 540
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 4 cut(s) 371, 496, 740, 764
NciI CCSGG 2 cut(s) 1174, 1175
NdeII GATC 3 cut(s) 433, 939, 1223
NlaIII CATG 4 cut(s) 110, 205, 956, 1007
NmuCI GTSAC 5 cut(s) 71, 722, 856, 871, 1214
NspI RCATGY 1 cut(s) 205
PciI ACATGT 1 cut(s) 201
PctI GAATGC 1 cut(s) 1021
PfeI GAWTC 7 cut(s) 23, 331, 356, 457, 533, 947, 998
PfoI TCCNGGA 1 cut(s) 177
PkrI GCNGC 2 cut(s) 451, 736
PleI GAGTC 2 cut(s) 172, 1075
PpsI GAGTC 2 cut(s) 172, 1075
PscI ACATGT 1 cut(s) 201
PshAI GACNNNNGTC 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 1043
Psp6I CCWGG 2 cut(s) 177, 538
PspEI GGTNACC 2 cut(s) 71, 519
PspGI CCWGG 2 cut(s) 177, 538
PspPI GGNCC 2 cut(s) 175, 1102
PstI CTGCAG 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 1 cut(s) 1223
RsaI GTAC 3 cut(s) 339, 408, 1030
RsaNI GTAC 3 cut(s) 338, 407, 1029
SacI GAGCTC 1 cut(s) 1043
SaqAI TTAA 8 cut(s) 230, 245, 260, 425, 593, 777, 1179, 1190
SatI GCNGC 2 cut(s) 450, 735
Sau3AI GATC 3 cut(s) 433, 939, 1223
Sau96I GGNCC 2 cut(s) 175, 1102
ScaI AGTACT 1 cut(s) 1030
SchI GAGTC 2 cut(s) 173, 1076
ScrFI CCNGG 4 cut(s) 179, 540, 1174, 1175
SduI GDGCHC 2 cut(s) 745, 1043
SfaNI GCATC 1 cut(s) 277
SfcI CTRYAG 2 cut(s) 289, 840
SinI GGWCC 1 cut(s) 175
SmaI CCCGGG 1 cut(s) 1175
SmlI CTYRAG 1 cut(s) 1016
SmoI CTYRAG 1 cut(s) 1016
Sse9I AATT 8 cut(s) 61, 112, 118, 257, 410, 594, 641, 702
SsiI CCGC 2 cut(s) 32, 131
SspMI CTAG 5 cut(s) 441, 453, 555, 720, 738
SstI GAGCTC 1 cut(s) 1043
StyD4I CCNGG 4 cut(s) 177, 538, 1172, 1173
StyI CCWWGG 1 cut(s) 440
TaaI ACNGT 5 cut(s) 418, 755, 834, 926, 1033
TaqI TCGA 3 cut(s) 279, 359, 699
TasI AATT 8 cut(s) 61, 112, 118, 257, 410, 594, 641, 702
TatI WGTACW 3 cut(s) 337, 406, 1028
TfiI GAWTC 7 cut(s) 23, 331, 356, 457, 533, 947, 998
Tru1I TTAA 8 cut(s) 230, 245, 260, 425, 593, 777, 1179, 1190
Tru9I TTAA 8 cut(s) 230, 245, 260, 425, 593, 777, 1179, 1190
TscAI CASTG 4 cut(s) 56, 298, 1000, 1015
TseFI GTSAC 5 cut(s) 71, 722, 856, 871, 1214
TseI GCWGC 2 cut(s) 449, 734
Tsp45I GTSAC 5 cut(s) 71, 722, 856, 871, 1214
TspDTI ATGAA 5 cut(s) 168, 634, 654, 1106, 1158
TspGWI ACGGA 2 cut(s) 644, 900
TspMI CCCGGG 1 cut(s) 1173
TspRI CASTG 4 cut(s) 56, 298, 1000, 1015
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 2 cut(s) 112, 641
XceI RCATGY 1 cut(s) 205
XmaI CCCGGG 1 cut(s) 1173
XmaJI CCTAGG 1 cut(s) 440
XmiI GTMKAC 2 cut(s) 420, 750
XspI CTAG 5 cut(s) 441, 453, 555, 720, 738
ZrmI AGTACT 1 cut(s) 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.