Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0013344

Physical Location & Seq

Genomic Coordinates Forward (+)

4499417 .. 4501008

Gene Structure 1592 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1056300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 516 bp

ATGGACGACGATGGGTCCAACCCATTGCGCCGAATGTCGAGTCGAACTCGAAAGGTTGCTTCGAAAATGACTGCTGCCCTCCAGAGCAGTGACAACCGCACGCAGGCTGCCCTTGCTCGGCTTGAAGCTTTGGAAAATGATAATGCTGGTTTGGAGACGGTAGAAGTTAACGACGATGATGAAGCTTCTCTTGACGATGATGATGTGTTTATAACAAAGAAGCAGTCTAAGGGCACCAAGCGTAAAACCCGGCAGGCAAAAGCTCTTGAGGCCAAGAAGGCCCCAAGAACATTCCTTGAGCTTCTGCATGAGGCAAATCTCGACTCCTTGCCTCCTCATGTTCCTTCTTATCTGAAGGCAGCAGTGGGACCACCAAGCTCTACCTCCCGGCGCCATTTCTGCACTGTTTGTGGTTCTGCTGCCACCTACACATGTGTGAGATGTGGGGTGCGATTTTGCTCGGGTCGTTGCCAGAATATACACAATGATACTCGATGTTTGAAGTTTGTTGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0000812 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006325 GO:0006338 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009909 GO:0009987 GO:0016043 GO:0019222 GO:0019899 GO:0031056 GO:0031063 GO:0031323 GO:0031399 GO:0031491 GO:0031974 GO:0031981 GO:0032268 GO:0032991 GO:0033043 GO:0033044 GO:0034728 GO:0040008 GO:0042742 GO:0042802 GO:0042826 GO:0043044 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043486 GO:0043933 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044877 GO:0048580 GO:0048638 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051239 GO:0051246 GO:0051276 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0070013 GO:0070603 GO:0071824 GO:0071840 GO:0080090 GO:0090311 GO:0097346 GO:0098542 GO:1902275 GO:1902494 GO:1904949 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-HIT

Protein Analysis

172

Amino Acids

18.79

Weight (kDa)

8.88

Isoelectric Point (pI)

50.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-HIT	PF04438	130 - 158	1.2e-09	HIT zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013344)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37055
fragaria_vesca	FvH4_3g39740
malus_domestica	MD03G1056300.v1.1 MD11G1057500.v1.1
prunus_persica	Prupe.6G048500_v2.0.a1 Prupe.6G048500_v2.0.a1
pyrus_communis	pycom03g04460
rosa_chinensis	RchiOBHm_Chr5g0071621
rosa_laevigata	RLG00000036233
rosa_multiflora	Rmu_co7965106.1_g000001 Rmu_sc0005745.1_g000030
rosa_roxburghii	Rroxscaffold_1G00009560
rosa_rugosa	Rorug05G0413600
rosa_samantha	Rh5AG469600 Rh5BG489000 Rh5CG512900 Rh5DG499600
rosa_wichuraiana	Rw5G043630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	212
AccB1I	GGYRCC	2 cut(s)	233, 390
AciI	CCGC	1 cut(s)	97
AcuI	CTGAAG	1 cut(s)	374
AcyI	GRCGYC	1 cut(s)	391
AfiI	CCNNNNNNNGG	2 cut(s)	103, 117
AflIII	ACRYGT	1 cut(s)	431
AgsI	TTSAA	2 cut(s)	125, 502
AhdI	GACNNNNNGTC	1 cut(s)	13
AleI	CACNNNNGTG	1 cut(s)	434
AluBI	AGCT	5 cut(s)	128, 185, 263, 301, 378
AluI	AGCT	5 cut(s)	128, 185, 263, 301, 378
Alw26I	GTCTC	1 cut(s)	149
Ama87I	CYCGRG	1 cut(s)	460
AoxI	GGCC	2 cut(s)	270, 279
ApeKI	GCWGC	4 cut(s)	74, 107, 359, 419
ArsI	GACNNNNNNTTYG	4 cut(s)	25, 57, 209, 241
AspLEI	GCGC	2 cut(s)	30, 393
AspS9I	GGNCC	3 cut(s)	15, 280, 368
AsuC2I	CCSGG	2 cut(s)	250, 388
AsuII	TTCGAA	1 cut(s)	62
AvaI	CYCGRG	1 cut(s)	460
AvaII	GGWCC	2 cut(s)	15, 368
BaeGI	GKGCMC	1 cut(s)	236
BanI	GGYRCC	2 cut(s)	233, 390
BbvI	GCAGC	4 cut(s)	61, 94, 371, 406
BccI	CCATC	1 cut(s)	5
BcnI	CCSGG	2 cut(s)	250, 388
BcoDI	GTCTC	1 cut(s)	149
BfoI	RGCGCY	1 cut(s)	394
BglI	GCCNNNNNGGC	1 cut(s)	278
BisI	GCNGC	4 cut(s)	75, 108, 360, 420
BlsI	GCNGC	4 cut(s)	76, 109, 361, 421
Bme1390I	CCNGG	2 cut(s)	250, 388
Bme18I	GGWCC	2 cut(s)	15, 368
BmeRI	GACNNNNNGTC	1 cut(s)	13
BmeT110I	CYCGRG	1 cut(s)	460
BmgT120I	GGNCC	3 cut(s)	15, 280, 368
BmiI	GGNNCC	5 cut(s)	16, 235, 282, 369, 392
BmrFI	CCNGG	2 cut(s)	250, 388
BplI	GAGNNNNNCTC	2 cut(s)	31, 63
BpmI	CTGGAG	1 cut(s)	65
Bpu14I	TTCGAA	1 cut(s)	62
BpuEI	CTTGAG	2 cut(s)	287, 317
BpuMI	CCSGG	2 cut(s)	250, 388
BsaHI	GRCGYC	1 cut(s)	391
Bsc4I	CCNNNNNNNGG	2 cut(s)	103, 117
Bse3DI	GCAATG	1 cut(s)	23
BseLI	CCNNNNNNNGG	2 cut(s)	103, 117
BseMI	GCAATG	1 cut(s)	23
BseRI	GAGGAG	1 cut(s)	324
BseSI	GKGCMC	1 cut(s)	236
BseXI	GCAGC	4 cut(s)	61, 94, 371, 406
BsgI	GTGCAG	1 cut(s)	385
BshFI	GGCC	2 cut(s)	272, 281
BshNI	GGYRCC	2 cut(s)	233, 390
BsiHKCI	CYCGRG	1 cut(s)	460
BsiSI	CCGG	2 cut(s)	250, 388
BslFI	GGGAC	1 cut(s)	381
BslI	CCNNNNNNNGG	2 cut(s)	103, 117
BsmAI	GTCTC	1 cut(s)	149
BsmBI	CGTCTC	1 cut(s)	149
BsmFI	GGGAC	1 cut(s)	381
BsnI	GGCC	2 cut(s)	272, 281
BsoBI	CYCGRG	1 cut(s)	460
Bsp119I	TTCGAA	1 cut(s)	62
Bsp1286I	GDGCHC	1 cut(s)	236
BspACI	CCGC	1 cut(s)	97
BspANI	GGCC	2 cut(s)	272, 281
BspLI	GGNNCC	5 cut(s)	16, 235, 282, 369, 392
BspT104I	TTCGAA	1 cut(s)	62
BspT107I	GGYRCC	2 cut(s)	233, 390
BsrDI	GCAATG	1 cut(s)	23
BssNI	GRCGYC	1 cut(s)	391
Bst4CI	ACNGT	2 cut(s)	160, 406
BstACI	GRCGYC	1 cut(s)	391
BstBI	TTCGAA	1 cut(s)	62
BstC8I	GCNNGC	3 cut(s)	101, 105, 255
BstDEI	CTNAG	1 cut(s)	228
BstH2I	RGCGCY	1 cut(s)	394
BstHHI	GCGC	2 cut(s)	30, 393
BstMAI	GTCTC	1 cut(s)	149
BstMWI	GCNNNNNNNGC	4 cut(s)	113, 269, 278, 399
BstNSI	RCATGY	1 cut(s)	435
BstSCI	CCNGG	2 cut(s)	248, 386
BstSLI	GKGCMC	1 cut(s)	236
BstV1I	GCAGC	4 cut(s)	61, 94, 371, 406
BsuRI	GGCC	2 cut(s)	272, 281
BtsI	GCAGTG	2 cut(s)	94, 369
BtsIMutI	CAGTG	3 cut(s)	94, 369, 402
Cac8I	GCNNGC	3 cut(s)	101, 105, 255
CfoI	GCGC	2 cut(s)	30, 393
Cfr13I	GGNCC	3 cut(s)	15, 280, 368
CviAII	CATG	3 cut(s)	308, 338, 432
CviJI	RGCY	9 cut(s)	107, 121, 128, 185, 263, 272, 281, 301, 378
CviKI_1	RGCY	9 cut(s)	107, 121, 128, 185, 263, 272, 281, 301, 378
DdeI	CTNAG	1 cut(s)	228
DinI	GGCGCC	1 cut(s)	392
DriI	GACNNNNNGTC	1 cut(s)	13
Eam1105I	GACNNNNNGTC	1 cut(s)	13
Eco47I	GGWCC	2 cut(s)	15, 368
Eco57I	CTGAAG	1 cut(s)	374
Eco88I	CYCGRG	1 cut(s)	460
EcoO109I	RGGNCCY	1 cut(s)	280
EgeI	GGCGCC	1 cut(s)	392
EheI	GGCGCC	1 cut(s)	392
Esp3I	CGTCTC	1 cut(s)	149
FaeI	CATG	3 cut(s)	311, 341, 435
FaiI	YATR	5 cut(s)	212, 309, 339, 433, 479
FalI	AAGNNNNNCTT	2 cut(s)	174, 206
FaqI	GGGAC	1 cut(s)	381
FatI	CATG	3 cut(s)	307, 337, 431
Fnu4HI	GCNGC	4 cut(s)	75, 108, 360, 420
Fsp4HI	GCNGC	4 cut(s)	75, 108, 360, 420
GlaI	GCGC	2 cut(s)	29, 392
GluI	GCNGC	4 cut(s)	75, 108, 360, 420
GsuI	CTGGAG	1 cut(s)	65
HaeII	RGCGCY	1 cut(s)	394
HaeIII	GGCC	2 cut(s)	272, 281
HapII	CCGG	2 cut(s)	250, 388
HhaI	GCGC	2 cut(s)	30, 393
Hin1I	GRCGYC	1 cut(s)	391
Hin1II	CATG	3 cut(s)	311, 341, 435
Hin6I	GCGC	2 cut(s)	28, 391
HinP1I	GCGC	2 cut(s)	28, 391
HincII	GTYRAC	1 cut(s)	169
HindII	GTYRAC	1 cut(s)	169
HindIII	AAGCTT	2 cut(s)	126, 183
HinfI	GANTC	2 cut(s)	40, 323
HpaI	GTTAAC	1 cut(s)	169
HpaII	CCGG	2 cut(s)	250, 388
Hpy166II	GTNNAC	1 cut(s)	169
Hpy188I	TCNGA	1 cut(s)	354
Hpy188III	TCNNGA	4 cut(s)	82, 191, 266, 320
Hpy8I	GTNNAC	1 cut(s)	169
Hpy99I	CGWCG	2 cut(s)	11, 176
HpyAV	CCTTC	3 cut(s)	271, 349, 354
HpyCH4III	ACNGT	2 cut(s)	160, 406
HpyCH4V	TGCA	2 cut(s)	307, 402
HpyF10VI	GCNNNNNNNGC	4 cut(s)	113, 269, 278, 399
HpyF3I	CTNAG	1 cut(s)	228
Hsp92I	GRCGYC	1 cut(s)	391
Hsp92II	CATG	3 cut(s)	311, 341, 435
HspAI	GCGC	2 cut(s)	28, 391
KasI	GGCGCC	1 cut(s)	390
KspAI	GTTAAC	1 cut(s)	169
LpnPI	CCDG	7 cut(s)	89, 95, 132, 239, 263, 401, 485
Lsp1109I	GCAGC	4 cut(s)	61, 94, 371, 406
MaeIII	GTNAC	1 cut(s)	89
MhlI	GDGCHC	1 cut(s)	236
Mly113I	GGCGCC	1 cut(s)	391
MlyI	GAGTC	2 cut(s)	49, 317
MmeI	TCCRAC	1 cut(s)	42
MnlI	CCTC	6 cut(s)	89, 262, 304, 342, 345, 394
MseI	TTAA	1 cut(s)	168
MslI	CAYNNNNRTG	1 cut(s)	434
MspI	CCGG	2 cut(s)	250, 388
MspR9I	CCNGG	2 cut(s)	250, 388
MwoI	GCNNNNNNNGC	4 cut(s)	113, 269, 278, 399
NarI	GGCGCC	1 cut(s)	391
NciI	CCSGG	2 cut(s)	250, 388
NlaIII	CATG	3 cut(s)	311, 341, 435
NlaIV	GGNNCC	5 cut(s)	16, 235, 282, 369, 392
NmeAIII	GCCGAG	1 cut(s)	97
NmuCI	GTSAC	1 cut(s)	89
NspI	RCATGY	1 cut(s)	435
NspV	TTCGAA	1 cut(s)	62
OliI	CACNNNNGTG	1 cut(s)	434
PciI	ACATGT	1 cut(s)	431
PkrI	GCNGC	4 cut(s)	76, 109, 361, 421
PleI	GAGTC	2 cut(s)	48, 317
PluTI	GGCGCC	1 cut(s)	394
PpsI	GAGTC	2 cut(s)	48, 317
PscI	ACATGT	1 cut(s)	431
PsiI	TTATAA	1 cut(s)	212
PspN4I	GGNNCC	5 cut(s)	16, 235, 282, 369, 392
PspPI	GGNCC	3 cut(s)	15, 280, 368
RseI	CAYNNNNRTG	1 cut(s)	434
SaqAI	TTAA	1 cut(s)	168
SatI	GCNGC	4 cut(s)	75, 108, 360, 420
Sau96I	GGNCC	3 cut(s)	15, 280, 368
SchI	GAGTC	2 cut(s)	49, 317
ScrFI	CCNGG	2 cut(s)	250, 388
SduI	GDGCHC	1 cut(s)	236
SetI	ASST	8 cut(s)	57, 130, 187, 265, 303, 380, 386, 428
SfiI	GGCCNNNNNGGCC	1 cut(s)	278
SfoI	GGCGCC	1 cut(s)	392
SfuI	TTCGAA	1 cut(s)	62
SinI	GGWCC	2 cut(s)	15, 368
SmiMI	CAYNNNNRTG	1 cut(s)	434
SmlI	CTYRAG	2 cut(s)	266, 296
SmoI	CTYRAG	2 cut(s)	266, 296
SsiI	CCGC	1 cut(s)	97
SspDI	GGCGCC	1 cut(s)	390
StyD4I	CCNGG	2 cut(s)	248, 386
TaaI	ACNGT	2 cut(s)	160, 406
TaqI	TCGA	6 cut(s)	38, 43, 49, 62, 321, 493
Tru1I	TTAA	1 cut(s)	168
Tru9I	TTAA	1 cut(s)	168
TscAI	CASTG	3 cut(s)	94, 369, 409
TseFI	GTSAC	1 cut(s)	89
TseI	GCWGC	4 cut(s)	74, 107, 359, 419
Tsp45I	GTSAC	1 cut(s)	89
TspDTI	ATGAA	1 cut(s)	195
TspRI	CASTG	3 cut(s)	94, 369, 409
VpaK11BI	GGWCC	2 cut(s)	15, 368
XceI	RCATGY	1 cut(s)	435

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.