MD03G1056400.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016555
Physical Location & Seq
Reverse (-)
4501429 .. 4504208
2780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1056400.v1.1.491

Sequence Viewer

Length: 1707 bp
ATGGAGGCTTCCACATTTACCCTTCTGCTTGGCCTTGCCTTCCTCACTTTCTTAGCTTCTTTCTCTGCTGCAGAAGATCACTTCCATGAATTCGTAGTTGAAGCAAAGCCGGTGAGGAGGCTGTGCAGAACCCACACCACAATCACGGTGAATGGTCAGTTCCCGGGACCAACTTTGGAAGTCAGAAATGGAGATTCCATTGCCGTCAAAGTTACCAACAGAGCAAAATACAATATCACCATCCACTGGCATGGACTGAAGCAGCAGAGAAATCCATGGGCAGATGGTCCTGAATACGTCACACAATGCCCTATCCAGCCAGGAGCTACTTACACATACAGGTTCACAATCACAGACCAAGAGGGCACTTTGTGGTGGCATGCTCACAGCAGATGGCTTAGAGCCACTGTCTACGGAGCCCTCATCATCTACCCTAGATTGGGTTCTCCCTACCCCTTCCCAATGCCGAAACGAGAAGTTCCCCTTCTTCTTGGGGAATGGTTTGATCGAAACCCCCTCGATGTCCAGAGGCTTGCGGCTTTCACAGGAGGAGCACCCAATGTCTCTGATGCATACACCATTAATGGCCAGCCTGGTGATCTTTACAGATGTTCCAAACAAGAAACCATTCGAATTCCAGTTGAATCGGGCGAGTCACTTCTCTTGAGAGTCGTCAACTCTGCACTCAATCAAGAACTCTTCTTTACTGTTGCCAACCACCAACTGACTGTTGTGAGCGCTGACGCAGCTTACACAAAACCTTTCACAACCAGAGTTATCATGCTAGGGCCTGGCCAAACAACCGATGTTCTGGTTACTGCTAATCAGCCCCCTGCTCGCTACTACGTGGCTGCTCGTGCCTATCAGACTGCCCAGAATGCGCCCTTCGACAATACCACCACCACTGCCATCCTTGAGTACAAGAATGCTGCTTGTTCAAAGAACGGCAAACCTTTAGCACCACTACTGCCTAGGTTGCCAGCTTACAACGACACAGCAACTTCTACTGCATTCACTGCTCAGCTAAAGAGCCCATCCCAGGTCAAAGTCCCCACACAGATTGACGAGAACCTGTATTTCATTGTGGGTTTAGGACTGAATAACTGCACAGTTCCTAACAGCCCTCGGTGTCAAGGACCAAATAACACCCGCTTTGCAGCTAGCATGAACAATGTTTCCTTTGTATTCCCAAGAAGCACCTCCTTAATGCAAGCCGCCTACAGTGGTGTACCTGGTGTCTTCACCACAGACTTTCCACCTGTTCCTCCTGTGCAATTCAATTATACCGGAAATGTGCCCCGGGGACTATGGTCACCTCGTCGTGGAACTAAGCTCTACAAGTTGAAGTATGGTGCTAATGTACAAATAGTGCTTCAGGACACCAGTATTGTGACAACTGAGGACCACCCTATGCATCTTCATGGGTACCATTTCTATGTGATTGGATCAGGATTTGGAAACTACAACCCCAGAACAGATCCAGCGAAATTCAACCTCATTGACCCGCCACAGAGGAATACCATCGGAACACCTCCAGGAGGATGGGTAGCTATTCGATTTAAGGCTGATAATCCAGGTGTCTGGCTGATGCATTGTCACTTAGATGCACATCTCTTCCTGGGTTTGGCAATGGCTTTTCTAGTCGAGAACGGAAACGGACCACTACAGTCTGTAATTCCTCCTCCAGCAGATCTACCGCGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

569

Amino Acids

62.82

Weight (kDa)

9.03

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 146 7.6e-46 Multicopper oxidase
Cu-oxidase PF00394 159 - 310 1.4e-42 Multicopper oxidase
Cu-oxidase_2 PF07731 417 - 551 1.6e-41 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30210
fragaria_vesca FvH4_3g39750
malus_domestica MD03G1056400.v1.1 MD11G1057600.v1.1 MD11G1058200.v1.1
pyrus_communis pycom03g04470 pycom11g04820
rosa_chinensis RchiOBHm_Chr5g0071631
rosa_laevigata RLG00000036231 RLG00000036234
rosa_multiflora Rmu_sc0005745.1_g000031
rosa_rugosa Rorug05G0413700
rosa_samantha Rh5CG513000
rosa_wichuraiana Rw5G043640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1425
AccB1I GGYRCC 1 cut(s) 1425
AccB7I CCANNNNNTGG 1 cut(s) 246
AccI GTMKAC 1 cut(s) 411
AccII CGCG 1 cut(s) 1699
AciI CCGC 5 cut(s) 536, 1150, 1215, 1505, 1697
AclWI GGATC 2 cut(s) 1453, 1472
AcoI YGGCCR 2 cut(s) 586, 793
AcsI RAATTY 3 cut(s) 89, 633, 1487
AcuI CTGAAG 2 cut(s) 278, 1358
AdeI CACNNNGTG 1 cut(s) 372
AfaI GTAC 4 cut(s) 920, 1230, 1362, 1427
AfeI AGCGCT 1 cut(s) 739
AfiI CCNNNNNNNGG 7 cut(s) 246, 439, 440, 1039, 1322, 1538, 1624
AgsI TTSAA 6 cut(s) 101, 644, 939, 1279, 1345, 1492
AjnI CCWGG 8 cut(s) 319, 592, 790, 1038, 1231, 1534, 1573, 1617
AloI GAACNNNNNNTCC 2 cut(s) 1160, 1192
AluBI AGCT 8 cut(s) 56, 326, 749, 983, 1024, 1160, 1333, 1550
AluI AGCT 8 cut(s) 56, 326, 749, 983, 1024, 1160, 1333, 1550
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 1 cut(s) 568
AlwI GGATC 2 cut(s) 1453, 1472
Ama87I CYCGRG 2 cut(s) 163, 1299
Aor51HI AGCGCT 1 cut(s) 739
AoxI GGCC 4 cut(s) 31, 586, 788, 793
ApeKI GCWGC 6 cut(s) 68, 262, 746, 851, 929, 1157
ApoI RAATTY 3 cut(s) 89, 633, 1487
AseI ATTAAT 1 cut(s) 582
Asp700I GAANNNNTTC 1 cut(s) 627
Asp718I GGTACC 1 cut(s) 1425
AspA2I CCTAGG 1 cut(s) 971
AspLEI GCGC 2 cut(s) 740, 883
AspS9I GGNCC 6 cut(s) 167, 287, 788, 1136, 1402, 1658
AsuC2I CCSGG 4 cut(s) 164, 165, 1300, 1301
AsuHPI GGTGA 6 cut(s) 124, 160, 229, 608, 1234, 1305
AsuII TTCGAA 1 cut(s) 631
AsuNHI GCTAGC 1 cut(s) 1160
AvaI CYCGRG 2 cut(s) 163, 1299
AvaII GGWCC 5 cut(s) 167, 287, 1136, 1402, 1658
AvrII CCTAGG 1 cut(s) 971
BaeGI GKGCMC 2 cut(s) 368, 1299
BalI TGGCCA 2 cut(s) 588, 795
BanI GGYRCC 1 cut(s) 1425
BanII GRGCYC 2 cut(s) 421, 1034
BauI CACGAG 1 cut(s) 855
BbsI GAAGAC 1 cut(s) 1231
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 6 cut(s) 55, 274, 758, 838, 916, 1169
BccI CCATC 7 cut(s) 248, 278, 387, 917, 1042, 1529, 1536
BceAI ACGGC 2 cut(s) 188, 961
BciT130I CCWGG 8 cut(s) 321, 594, 792, 1040, 1233, 1536, 1575, 1619
BcnI CCSGG 4 cut(s) 164, 165, 1300, 1301
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 5 cut(s) 435, 785, 972, 1161, 1640
BfmI CTRYAG 3 cut(s) 69, 1219, 1664
BfoI RGCGCY 1 cut(s) 741
BglII AGATCT 1 cut(s) 1690
BisI GCNGC 8 cut(s) 69, 263, 537, 747, 852, 930, 1158, 1215
BlnI CCTAGG 1 cut(s) 971
BlpI GCTNAGC 1 cut(s) 1020
BlsI GCNGC 8 cut(s) 70, 264, 538, 748, 853, 931, 1159, 1216
Bme18I GGWCC 5 cut(s) 167, 287, 1136, 1402, 1658
BmeT110I CYCGRG 2 cut(s) 163, 1299
BmgT120I GGNCC 6 cut(s) 167, 287, 788, 1136, 1402, 1658
BmiI GGNNCC 3 cut(s) 168, 418, 1427
BmsI GCATC 5 cut(s) 559, 1423, 1578, 1594, 1691
BmtI GCTAGC 1 cut(s) 1164
BoxI GACNNNNGTC 1 cut(s) 1309
BpiI GAAGAC 1 cut(s) 1231
BpmI CTGGAG 2 cut(s) 1518, 1668
Bpu1102I GCTNAGC 1 cut(s) 1020
Bpu14I TTCGAA 1 cut(s) 631
BpuEI CTTGAG 2 cut(s) 685, 935
BpuMI CCSGG 4 cut(s) 164, 165, 1300, 1301
BsaAI YACGTR 1 cut(s) 847
BsaBI GATNNNNATC 1 cut(s) 1608
BsaJI CCNNGG 9 cut(s) 163, 275, 971, 1038, 1124, 1298, 1299, 1300, 1618
BsaWI WCCGGW 1 cut(s) 1286
BsaXI ACNNNNNCTCC 2 cut(s) 1530, 1560
Bsc4I CCNNNNNNNGG 7 cut(s) 246, 439, 440, 1039, 1322, 1538, 1624
Bse118I RCCGGY 1 cut(s) 109
Bse1I ACTGG 3 cut(s) 251, 638, 1383
Bse3DI GCAATG 2 cut(s) 198, 1635
Bse8I GATNNNNATC 1 cut(s) 1608
BseBI CCWGG 8 cut(s) 321, 594, 792, 1040, 1233, 1536, 1575, 1619
BseDI CCNNGG 9 cut(s) 163, 275, 971, 1038, 1124, 1298, 1299, 1300, 1618
BseGI GGATG 4 cut(s) 240, 909, 1034, 1547
BseJI GATNNNNATC 1 cut(s) 1608
BseLI CCNNNNNNNGG 7 cut(s) 246, 439, 440, 1039, 1322, 1538, 1624
BseMI GCAATG 2 cut(s) 198, 1635
BseMII CTCAG 2 cut(s) 1034, 1389
BseNI ACTGG 3 cut(s) 251, 638, 1383
BseRI GAGGAG 3 cut(s) 130, 564, 1671
BseSI GKGCMC 2 cut(s) 368, 1299
BseXI GCAGC 6 cut(s) 55, 274, 758, 838, 916, 1169
BsgI GTGCAG 3 cut(s) 145, 666, 1090
Bsh1236I CGCG 1 cut(s) 1699
BshFI GGCC 4 cut(s) 33, 588, 790, 795
BshNI GGYRCC 1 cut(s) 1425
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 2 cut(s) 163, 1299
BsiSI CCGG 4 cut(s) 110, 164, 1287, 1300
BslFI GGGAC 3 cut(s) 180, 1034, 1317
BslI CCNNNNNNNGG 7 cut(s) 246, 439, 440, 1039, 1322, 1538, 1624
BsmAI GTCTC 1 cut(s) 568
BsmFI GGGAC 3 cut(s) 180, 1034, 1317
BsmI GAATGC 3 cut(s) 883, 931, 1010
BsnI GGCC 4 cut(s) 33, 588, 790, 795
BsoBI CYCGRG 2 cut(s) 163, 1299
Bsp119I TTCGAA 1 cut(s) 631
Bsp1286I GDGCHC 5 cut(s) 368, 421, 556, 1034, 1299
Bsp1407I TGTACA 1 cut(s) 1360
Bsp143I GATC 6 cut(s) 76, 505, 598, 1445, 1477, 1690
Bsp1720I GCTNAGC 1 cut(s) 1020
Bsp19I CCATGG 1 cut(s) 275
BspACI CCGC 5 cut(s) 536, 1150, 1215, 1505, 1697
BspANI GGCC 4 cut(s) 33, 588, 790, 795
BspCNI CTCAG 2 cut(s) 1033, 1390
BspFNI CGCG 1 cut(s) 1699
BspLI GGNNCC 3 cut(s) 168, 418, 1427
BspMAI CTGCAG 1 cut(s) 73
BspOI GCTAGC 1 cut(s) 1164
BspPI GGATC 2 cut(s) 1453, 1472
BspT104I TTCGAA 1 cut(s) 631
BspT107I GGYRCC 1 cut(s) 1425
BsrDI GCAATG 2 cut(s) 198, 1635
BsrFI RCCGGY 1 cut(s) 109
BsrGI TGTACA 1 cut(s) 1360
BsrI ACTGG 3 cut(s) 251, 638, 1383
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 9 cut(s) 163, 275, 971, 1038, 1124, 1298, 1299, 1300, 1618
BssMI GATC 6 cut(s) 76, 505, 598, 1445, 1477, 1690
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 2 cut(s) 275, 971
Bst2BI CACGAG 1 cut(s) 855
Bst2UI CCWGG 8 cut(s) 321, 594, 792, 1040, 1233, 1536, 1575, 1619
Bst4CI ACNGT 7 cut(s) 148, 409, 709, 730, 1111, 1223, 1668
Bst6I CTCTTC 2 cut(s) 704, 1619
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstAUI TGTACA 1 cut(s) 1360
BstBAI YACGTR 1 cut(s) 847
BstBI TTCGAA 1 cut(s) 631
BstC8I GCNNGC 7 cut(s) 381, 534, 590, 838, 981, 1162, 1212
BstDEI CTNAG 6 cut(s) 52, 398, 1020, 1329, 1398, 1600
BstDSI CCRYGG 1 cut(s) 275
BstEII GGTNACC 1 cut(s) 1311
BstENI CCTNNNNNAGG 1 cut(s) 1536
BstF5I GGATG 4 cut(s) 240, 909, 1034, 1547
BstFNI CGCG 1 cut(s) 1699
BstH2I RGCGCY 1 cut(s) 741
BstHHI GCGC 2 cut(s) 740, 883
BstKTI GATC 6 cut(s) 79, 508, 601, 1448, 1480, 1693
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 6 cut(s) 76, 505, 598, 1445, 1477, 1690
BstMWI GCNNNNNNNGC 5 cut(s) 746, 857, 878, 976, 1016
BstNI CCWGG 8 cut(s) 321, 594, 792, 1040, 1233, 1536, 1575, 1619
BstNSI RCATGY 1 cut(s) 383
BstPAI GACNNNNGTC 1 cut(s) 1309
BstPI GGTNACC 1 cut(s) 1311
BstSFI CTRYAG 3 cut(s) 69, 1219, 1664
BstSLI GKGCMC 2 cut(s) 368, 1299
BstUI CGCG 1 cut(s) 1699
BstV1I GCAGC 6 cut(s) 55, 274, 758, 838, 916, 1169
BstV2I GAAGAC 1 cut(s) 1231
BstX2I RGATCY 2 cut(s) 1477, 1690
BstXI CCANNNNNNTGG 3 cut(s) 251, 1542, 1581
BstYI RGATCY 2 cut(s) 1477, 1690
BsuRI GGCC 4 cut(s) 33, 588, 790, 795
BtgI CCRYGG 1 cut(s) 275
BtsCI GGATG 4 cut(s) 240, 909, 1034, 1547
BtsI GCAGTG 2 cut(s) 903, 1014
BtsIMutI CAGTG 5 cut(s) 244, 405, 903, 1014, 1228
Cac8I GCNNGC 7 cut(s) 381, 534, 590, 838, 981, 1162, 1212
CfoI GCGC 2 cut(s) 740, 883
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 6 cut(s) 167, 287, 788, 1136, 1402, 1658
Cfr9I CCCGGG 2 cut(s) 163, 1299
CseI GACGC 1 cut(s) 752
CsiI ACCWGGT 1 cut(s) 1231
Csp6I GTAC 4 cut(s) 919, 1229, 1361, 1426
CspCI CAANNNNNGTGG 2 cut(s) 1042, 1077
CviAII CATG 7 cut(s) 86, 251, 276, 380, 781, 1165, 1421
CviQI GTAC 4 cut(s) 919, 1229, 1361, 1426
DdeI CTNAG 6 cut(s) 52, 398, 1020, 1329, 1398, 1600
DpnI GATC 6 cut(s) 78, 507, 600, 1447, 1479, 1692
DpnII GATC 6 cut(s) 76, 505, 598, 1445, 1477, 1690
DraIII CACNNNGTG 1 cut(s) 372
EaeI YGGCCR 2 cut(s) 586, 793
Eam1104I CTCTTC 2 cut(s) 704, 1619
EarI CTCTTC 2 cut(s) 704, 1619
Eco130I CCWWGG 2 cut(s) 275, 971
Eco24I GRGCYC 2 cut(s) 421, 1034
Eco47I GGWCC 5 cut(s) 167, 287, 1136, 1402, 1658
Eco47III AGCGCT 1 cut(s) 739
Eco57I CTGAAG 2 cut(s) 278, 1358
Eco88I CYCGRG 2 cut(s) 163, 1299
Eco91I GGTNACC 1 cut(s) 1311
EcoNI CCTNNNNNAGG 1 cut(s) 1536
EcoO109I RGGNCCY 1 cut(s) 788
EcoO65I GGTNACC 1 cut(s) 1311
EcoRI GAATTC 2 cut(s) 89, 633
EcoRII CCWGG 8 cut(s) 319, 592, 790, 1038, 1231, 1534, 1573, 1617
EcoT14I CCWWGG 2 cut(s) 275, 971
EcoT22I ATGCAT 3 cut(s) 574, 1416, 1593
EcoT38I GRGCYC 2 cut(s) 421, 1034
ErhI CCWWGG 2 cut(s) 275, 971
FaeI CATG 7 cut(s) 89, 254, 279, 383, 784, 1168, 1424
FalI AAGNNNNNCTT 2 cut(s) 468, 500
FaqI GGGAC 3 cut(s) 180, 1034, 1317
FatI CATG 7 cut(s) 85, 250, 275, 379, 780, 1164, 1420
FauI CCCGC 2 cut(s) 1157, 1512
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 8 cut(s) 69, 263, 537, 747, 852, 930, 1158, 1215
FokI GGATG 4 cut(s) 227, 896, 1021, 1554
FriOI GRGCYC 2 cut(s) 421, 1034
Fsp4HI GCNGC 8 cut(s) 69, 263, 537, 747, 852, 930, 1158, 1215
FspBI CTAG 5 cut(s) 435, 785, 972, 1161, 1640
GlaI GCGC 2 cut(s) 739, 882
GluI GCNGC 8 cut(s) 69, 263, 537, 747, 852, 930, 1158, 1215
GsuI CTGGAG 2 cut(s) 1518, 1668
HaeII RGCGCY 1 cut(s) 741
HaeIII GGCC 4 cut(s) 33, 588, 790, 795
HapII CCGG 4 cut(s) 110, 164, 1287, 1300
HgaI GACGC 1 cut(s) 752
HhaI GCGC 2 cut(s) 740, 883
Hin1II CATG 7 cut(s) 89, 254, 279, 383, 784, 1168, 1424
Hin6I GCGC 2 cut(s) 738, 881
HinP1I GCGC 2 cut(s) 738, 881
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HinfI GANTC 4 cut(s) 194, 644, 653, 669
HpaII CCGG 4 cut(s) 110, 164, 1287, 1300
HphI GGTGA 6 cut(s) 124, 160, 229, 608, 1234, 1305
Hpy166II GTNNAC 4 cut(s) 345, 412, 676, 1229
Hpy188I TCNGA 4 cut(s) 185, 568, 867, 1526
Hpy188III TCNNGA 7 cut(s) 290, 526, 664, 692, 1376, 1449, 1645
Hpy8I GTNNAC 4 cut(s) 345, 412, 676, 1229
Hpy99I CGWCG 1 cut(s) 1323
HpyAV CCTTC 5 cut(s) 32, 49, 466, 494, 895
HpyCH4III ACNGT 7 cut(s) 148, 409, 709, 730, 1111, 1223, 1668
HpyCH4IV ACGT 2 cut(s) 297, 846
HpyF10VI GCNNNNNNNGC 5 cut(s) 746, 857, 878, 976, 1016
HpyF3I CTNAG 6 cut(s) 52, 398, 1020, 1329, 1398, 1600
HpySE526I ACGT 2 cut(s) 297, 846
Hsp92II CATG 7 cut(s) 89, 254, 279, 383, 784, 1168, 1424
HspAI GCGC 2 cut(s) 738, 881
KpnI GGTACC 1 cut(s) 1429
Kzo9I GATC 6 cut(s) 76, 505, 598, 1445, 1477, 1690
LmnI GCTCC 3 cut(s) 323, 416, 551
Lsp1109I GCAGC 6 cut(s) 55, 274, 758, 838, 916, 1169
LweI GCATC 5 cut(s) 559, 1423, 1578, 1594, 1691
MabI ACCWGGT 1 cut(s) 1231
MaeI CTAG 5 cut(s) 435, 785, 972, 1161, 1640
MaeII ACGT 2 cut(s) 297, 846
MaeIII GTNAC 7 cut(s) 211, 298, 654, 814, 1311, 1390, 1595
MalI GATC 6 cut(s) 78, 507, 600, 1447, 1479, 1692
MboI GATC 6 cut(s) 76, 505, 598, 1445, 1477, 1690
MboII GAAGA 6 cut(s) 86, 479, 691, 1231, 1409, 1606
MflI RGATCY 2 cut(s) 1477, 1690
MhlI GDGCHC 5 cut(s) 368, 421, 556, 1034, 1299
MlsI TGGCCA 2 cut(s) 588, 795
MluCI AATT 6 cut(s) 89, 633, 1274, 1279, 1487, 1674
MluNI TGGCCA 2 cut(s) 588, 795
MlyI GAGTC 2 cut(s) 662, 678
Mox20I TGGCCA 2 cut(s) 588, 795
Mph1103I ATGCAT 3 cut(s) 574, 1416, 1593
MroXI GAANNNNTTC 1 cut(s) 627
MscI TGGCCA 2 cut(s) 588, 795
MseI TTAA 3 cut(s) 582, 1205, 1560
MslI CAYNNNNRTG 5 cut(s) 84, 249, 1419, 1434, 1602
Msp20I TGGCCA 2 cut(s) 588, 795
MspI CCGG 4 cut(s) 110, 164, 1287, 1300
Mva1269I GAATGC 3 cut(s) 883, 931, 1010
MvaI CCWGG 8 cut(s) 321, 594, 792, 1040, 1233, 1536, 1575, 1619
MvnI CGCG 1 cut(s) 1699
MwoI GCNNNNNNNGC 5 cut(s) 746, 857, 878, 976, 1016
NciI CCSGG 4 cut(s) 164, 165, 1300, 1301
NcoI CCATGG 1 cut(s) 275
NdeII GATC 6 cut(s) 76, 505, 598, 1445, 1477, 1690
NheI GCTAGC 1 cut(s) 1160
NlaIII CATG 7 cut(s) 89, 254, 279, 383, 784, 1168, 1424
NlaIV GGNNCC 3 cut(s) 168, 418, 1427
NmuCI GTSAC 5 cut(s) 298, 654, 1311, 1390, 1595
NsiI ATGCAT 3 cut(s) 574, 1416, 1593
NspI RCATGY 1 cut(s) 383
NspV TTCGAA 1 cut(s) 631
PaeI GCATGC 1 cut(s) 383
PctI GAATGC 3 cut(s) 883, 931, 1010
PdmI GAANNNNTTC 1 cut(s) 627
PfeI GAWTC 2 cut(s) 194, 644
PflMI CCANNNNNTGG 1 cut(s) 246
PfoI TCCNGGA 1 cut(s) 1534
PkrI GCNGC 8 cut(s) 70, 264, 538, 748, 853, 931, 1159, 1216
PleI GAGTC 2 cut(s) 661, 677
PpsI GAGTC 2 cut(s) 661, 677
Ppu21I YACGTR 1 cut(s) 847
PshAI GACNNNNGTC 1 cut(s) 1309
PshBI ATTAAT 1 cut(s) 582
Psp6I CCWGG 8 cut(s) 319, 592, 790, 1038, 1231, 1534, 1573, 1617
PspEI GGTNACC 1 cut(s) 1311
PspGI CCWGG 8 cut(s) 319, 592, 790, 1038, 1231, 1534, 1573, 1617
PspN4I GGNNCC 3 cut(s) 168, 418, 1427
PspPI GGNCC 6 cut(s) 167, 287, 788, 1136, 1402, 1658
PstI CTGCAG 1 cut(s) 73
PsuI RGATCY 2 cut(s) 1477, 1690
RsaI GTAC 4 cut(s) 920, 1230, 1362, 1427
RsaNI GTAC 4 cut(s) 919, 1229, 1361, 1426
RseI CAYNNNNRTG 5 cut(s) 84, 249, 1419, 1434, 1602
SaqAI TTAA 3 cut(s) 582, 1205, 1560
SatI GCNGC 8 cut(s) 69, 263, 537, 747, 852, 930, 1158, 1215
Sau3AI GATC 6 cut(s) 76, 505, 598, 1445, 1477, 1690
Sau96I GGNCC 6 cut(s) 167, 287, 788, 1136, 1402, 1658
SchI GAGTC 2 cut(s) 662, 678
SduI GDGCHC 5 cut(s) 368, 421, 556, 1034, 1299
SexAI ACCWGGT 1 cut(s) 1231
SfaNI GCATC 5 cut(s) 559, 1423, 1578, 1594, 1691
SfcI CTRYAG 3 cut(s) 69, 1219, 1664
SfuI TTCGAA 1 cut(s) 631
SinI GGWCC 5 cut(s) 167, 287, 1136, 1402, 1658
SmaI CCCGGG 2 cut(s) 165, 1301
SmiMI CAYNNNNRTG 5 cut(s) 84, 249, 1419, 1434, 1602
SmlI CTYRAG 2 cut(s) 664, 914
SmoI CTYRAG 2 cut(s) 664, 914
SphI GCATGC 1 cut(s) 383
Sse9I AATT 6 cut(s) 89, 633, 1274, 1279, 1487, 1674
SsiI CCGC 5 cut(s) 536, 1150, 1215, 1505, 1697
SspMI CTAG 5 cut(s) 435, 785, 972, 1161, 1640
StyI CCWWGG 2 cut(s) 275, 971
TaaI ACNGT 7 cut(s) 148, 409, 709, 730, 1111, 1223, 1668
TaiI ACGT 2 cut(s) 300, 849
TaqI TCGA 6 cut(s) 508, 519, 631, 888, 1555, 1644
TasI AATT 6 cut(s) 89, 633, 1274, 1279, 1487, 1674
TatI WGTACW 2 cut(s) 918, 1360
TauI GCSGC 2 cut(s) 539, 1217
TfiI GAWTC 2 cut(s) 194, 644
Tru1I TTAA 3 cut(s) 582, 1205, 1560
Tru9I TTAA 3 cut(s) 582, 1205, 1560
TscAI CASTG 5 cut(s) 251, 412, 910, 1021, 1228
TseFI GTSAC 5 cut(s) 298, 654, 1311, 1390, 1595
TseI GCWGC 6 cut(s) 68, 262, 746, 851, 929, 1157
Tsp45I GTSAC 5 cut(s) 298, 654, 1311, 1390, 1595
TspDTI ATGAA 4 cut(s) 102, 1069, 1181, 1409
TspGWI ACGGA 3 cut(s) 429, 1665, 1671
TspMI CCCGGG 2 cut(s) 163, 1299
TspRI CASTG 5 cut(s) 251, 412, 910, 1021, 1228
Van91I CCANNNNNTGG 1 cut(s) 246
VpaK11BI GGWCC 5 cut(s) 167, 287, 1136, 1402, 1658
VspI ATTAAT 1 cut(s) 582
XagI CCTNNNNNAGG 1 cut(s) 1536
XapI RAATTY 3 cut(s) 89, 633, 1487
XceI RCATGY 1 cut(s) 383
XmaI CCCGGG 2 cut(s) 163, 1299
XmaJI CCTAGG 1 cut(s) 971
XmiI GTMKAC 1 cut(s) 411
XmnI GAANNNNTTC 1 cut(s) 627
XspI CTAG 5 cut(s) 435, 785, 972, 1161, 1640
Zsp2I ATGCAT 3 cut(s) 574, 1416, 1593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.