pycom03g04470

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0016555
Physical Location & Seq
Reverse (-)
3486875 .. 3489943
3069 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04470.2

Sequence Viewer

Length: 1809 bp
ATGGTGGAGGTGATAAGGAAATATATAAATAGGTCCTCCCTCACAAGTCTAAGCTCACACCATCACTACTGCTGCTACTACAAGTTAATTCACAAAGCAATGGAGGCTTCCACATTTACCCTTCTGCTTGGCCTTCTTGCCTTCCTCACTTTCTTAGCTTCTTTCTCTGCTGCAGAAGATCACTTCCATGAATTCATAGTTGAAGCAAAGCCAGTGAGGAGGCTGTGCAGAACCCACACCACAATCACAGTAAATGGTCAGTTCCCGGGACCAACTTTGGAAGTCAGAAATGGAGATTCCGTTGCCGTCCAAGTTACCAACAGGGCAAAATACAATATCACCATCCACTGGCATGGACTGAAGCAGCAGAGAAATCCATGGGCTGATGGTCCTGAATACGTCACACAGTGCCCTATCCAGCCAGGAGCTACTTATACATACAGGTTCACAATCACAGACCAAGAGGGCACTTTGTGGTGGCATGCTCACAGCAGATGGCTTAGAGCCACTGTCTACGGAGCCCTCATCATCTACCCTAGATTGGGTTCTCCCTACCCCTTCTCAATGCCGAAACAAGAAGTTCCCCTTCTTCTTGGGGAATGGTTTGATCGAAACCCCCTTGATGTCCAAAGGCTTGCGGCTTTCACAGGAGGAGCACCTAATGTCTCTGATGCATACACCATTAATGGCCAGCCTGGTGATCTTTACAGATGTTCCAAACAAGAAACCATTCGAATTCCAGTTGAATCGGGCGAGTCACTTCTCCTGAGAGTCGTCAACTCTGCACTCAATCAAGAACTCTTCTTTACTGTTGCCAACCACCAACTGACTGTTGTGAGCGCTGACGCAGCTTACACAAAACCTTTCACAACCAGAGTTATCATGCTAGGGCCTGGCCAAACAACCGATGTTCTGGTTACTGCTGATCAGCCCCCAGCTCGCTACTACGTGGCTGCTCGTGCCTATCAGACTGCCCAGAATGCACCCTTCGACAATACCACCACCACTGCTATCCTTGAGTACAAGAATGCTGCTTGTTCAAAGAATGGGAAACCTTTAGCACCACTATTGCCTAGATTGCCAGCTTACAACGACACAGCAACTTCTACTGCATTCACTGCTCAGCTAAAGAGCCCATCCCAGGTCAAAGTCCCCATGCAGATTGACGAGAACCTGTATTTCATTGTGGGTTTAGGACTGAATAATTGCACAGTTCCTAACAGCCCTCGGTGTCAAGGACCAAATAACACCCGCTTTGCAGCTAGCATGAACAATGTTTCCTTTGTATTCCCAAGAAGCACCTCCTTAATGCAAGCCGCCTACAGTGGTGTACCTGGTGTCTTCACCACAGACTTTCCACCTGTTCCTCCTGTGCAATTCAACTATACCGGAAATGTGCCCCGGGGACTATGGTCACCTCGTCGTGGAACTAAGCTCTACAAATTGAAGTATGATGCTAATGTACAAATAGTGCTTCAGGACACCAGTATTGTGACAACTGAGGACCACCCTATGCATCTTCATGGGTACCATTTCTACGTGATTGGATCAGGATTTGGAAACTACAACCCCAGAACAGATCCAGCGAAATTCAACCTCGTTGACCCGCCACAAAGGAATACCATTGGAACACCTCCAGGAGGATGGGTAGCTATTCGATTTAAGGCTGATAATCCAGGTGTCTGGTTGATGCATTGTCACTTAGATGCACATCTCTTCCTGGGTTTGGCAATGGCTTTTCTAGTCGAGAACGGAAACGGACCACTACAGTCTGTAATTCCTCCTCCAGTAGATCTACCACGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

603

Amino Acids

66.96

Weight (kDa)

8.92

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30210
fragaria_vesca FvH4_3g39750
malus_domestica MD03G1056400.v1.1 MD11G1057600.v1.1 MD11G1058200.v1.1
pyrus_communis pycom03g04470 pycom11g04820
rosa_chinensis RchiOBHm_Chr5g0071631
rosa_laevigata RLG00000036231 RLG00000036234
rosa_multiflora Rmu_sc0005745.1_g000031
rosa_rugosa Rorug05G0413700
rosa_samantha Rh5CG513000
rosa_wichuraiana Rw5G043640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1527
AccB1I GGYRCC 1 cut(s) 1527
AccB7I CCANNNNNTGG 1 cut(s) 348
AccI GTMKAC 1 cut(s) 513
AciI CCGC 4 cut(s) 638, 1252, 1317, 1607
AclWI GGATC 2 cut(s) 1555, 1574
AcoI YGGCCR 2 cut(s) 688, 895
AcsI RAATTY 3 cut(s) 191, 735, 1589
AcuI CTGAAG 2 cut(s) 380, 1460
AdeI CACNNNGTG 2 cut(s) 408, 474
AfaI GTAC 4 cut(s) 1022, 1332, 1464, 1529
AfeI AGCGCT 1 cut(s) 841
AfiI CCNNNNNNNGG 7 cut(s) 348, 541, 542, 1141, 1424, 1640, 1726
AgsI TTSAA 6 cut(s) 203, 746, 1041, 1381, 1447, 1594
AjnI CCWGG 8 cut(s) 421, 694, 892, 1140, 1333, 1636, 1675, 1719
AloI GAACNNNNNNTCC 2 cut(s) 1262, 1294
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 1 cut(s) 670
AlwI GGATC 2 cut(s) 1555, 1574
Ama87I CYCGRG 2 cut(s) 265, 1401
Aor51HI AGCGCT 1 cut(s) 841
AoxI GGCC 4 cut(s) 130, 688, 890, 895
ApeKI GCWGC 7 cut(s) 72, 170, 364, 848, 953, 1031, 1259
ApoI RAATTY 3 cut(s) 191, 735, 1589
AseI ATTAAT 1 cut(s) 684
Asp700I GAANNNNTTC 1 cut(s) 729
Asp718I GGTACC 1 cut(s) 1527
AspLEI GCGC 1 cut(s) 842
AspS9I GGNCC 7 cut(s) 33, 269, 389, 890, 1238, 1504, 1760
AsuC2I CCSGG 4 cut(s) 266, 267, 1402, 1403
AsuHPI GGTGA 5 cut(s) 22, 331, 710, 1336, 1407
AsuII TTCGAA 1 cut(s) 733
AsuNHI GCTAGC 1 cut(s) 1262
AvaI CYCGRG 2 cut(s) 265, 1401
AvaII GGWCC 6 cut(s) 33, 269, 389, 1238, 1504, 1760
BaeGI GKGCMC 3 cut(s) 413, 470, 1401
BalI TGGCCA 2 cut(s) 690, 897
BanI GGYRCC 1 cut(s) 1527
BanII GRGCYC 2 cut(s) 523, 1136
BauI CACGAG 1 cut(s) 957
BbsI GAAGAC 1 cut(s) 1333
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 7 cut(s) 59, 157, 376, 860, 940, 1018, 1271
BccI CCATC 6 cut(s) 69, 350, 380, 489, 1144, 1638
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 8 cut(s) 423, 696, 894, 1142, 1335, 1638, 1677, 1721
BclI TGATCA 1 cut(s) 925
BcnI CCSGG 4 cut(s) 266, 267, 1402, 1403
BcoDI GTCTC 1 cut(s) 670
BfaI CTAG 5 cut(s) 537, 887, 1074, 1263, 1742
BfmI CTRYAG 3 cut(s) 171, 1321, 1766
BfoI RGCGCY 1 cut(s) 843
BglII AGATCT 1 cut(s) 1792
BisI GCNGC 9 cut(s) 73, 171, 365, 639, 849, 954, 1032, 1260, 1317
BlpI GCTNAGC 1 cut(s) 1122
BlsI GCNGC 9 cut(s) 74, 172, 366, 640, 850, 955, 1033, 1261, 1318
Bme18I GGWCC 6 cut(s) 33, 269, 389, 1238, 1504, 1760
BmeT110I CYCGRG 2 cut(s) 265, 1401
BmgT120I GGNCC 7 cut(s) 33, 269, 389, 890, 1238, 1504, 1760
BmiI GGNNCC 3 cut(s) 270, 520, 1529
BmsI GCATC 6 cut(s) 661, 1444, 1525, 1680, 1696, 1793
BmtI GCTAGC 1 cut(s) 1266
BoxI GACNNNNGTC 1 cut(s) 1411
BpiI GAAGAC 1 cut(s) 1333
BpmI CTGGAG 2 cut(s) 1620, 1770
Bpu1102I GCTNAGC 1 cut(s) 1122
Bpu14I TTCGAA 1 cut(s) 733
BpuEI CTTGAG 1 cut(s) 1037
BpuMI CCSGG 4 cut(s) 266, 267, 1402, 1403
BsaAI YACGTR 2 cut(s) 949, 1540
BsaBI GATNNNNATC 1 cut(s) 1710
BsaJI CCNNGG 8 cut(s) 265, 377, 1140, 1226, 1400, 1401, 1402, 1720
BsaWI WCCGGW 1 cut(s) 1388
BsaXI ACNNNNNCTCC 4 cut(s) 285, 315, 1632, 1662
Bsc4I CCNNNNNNNGG 7 cut(s) 348, 541, 542, 1141, 1424, 1640, 1726
Bse1I ACTGG 5 cut(s) 212, 353, 740, 1485, 1787
Bse3DI GCAATG 2 cut(s) 105, 1737
Bse8I GATNNNNATC 1 cut(s) 1710
BseBI CCWGG 8 cut(s) 423, 696, 894, 1142, 1335, 1638, 1677, 1721
BseDI CCNNGG 8 cut(s) 265, 377, 1140, 1226, 1400, 1401, 1402, 1720
BseGI GGATG 3 cut(s) 342, 1136, 1649
BseJI GATNNNNATC 1 cut(s) 1710
BseLI CCNNNNNNNGG 7 cut(s) 348, 541, 542, 1141, 1424, 1640, 1726
BseMI GCAATG 2 cut(s) 105, 1737
BseMII CTCAG 3 cut(s) 758, 1136, 1491
BseNI ACTGG 5 cut(s) 212, 353, 740, 1485, 1787
BseRI GAGGAG 3 cut(s) 232, 666, 1773
BseSI GKGCMC 3 cut(s) 413, 470, 1401
BseXI GCAGC 7 cut(s) 59, 157, 376, 860, 940, 1018, 1271
BseYI CCCAGC 1 cut(s) 934
BsgI GTGCAG 2 cut(s) 247, 768
BshFI GGCC 4 cut(s) 132, 690, 892, 897
BshNI GGYRCC 1 cut(s) 1527
BsiHKAI GWGCWC 1 cut(s) 658
BsiHKCI CYCGRG 2 cut(s) 265, 1401
BsiSI CCGG 3 cut(s) 266, 1389, 1402
BslFI GGGAC 3 cut(s) 282, 1136, 1419
BslI CCNNNNNNNGG 7 cut(s) 348, 541, 542, 1141, 1424, 1640, 1726
BsmAI GTCTC 1 cut(s) 670
BsmFI GGGAC 3 cut(s) 282, 1136, 1419
BsmI GAATGC 3 cut(s) 985, 1033, 1112
BsnI GGCC 4 cut(s) 132, 690, 892, 897
BsoBI CYCGRG 2 cut(s) 265, 1401
Bsp119I TTCGAA 1 cut(s) 733
Bsp1286I GDGCHC 6 cut(s) 413, 470, 523, 658, 1136, 1401
Bsp1407I TGTACA 1 cut(s) 1462
Bsp143I GATC 7 cut(s) 178, 607, 700, 925, 1547, 1579, 1792
Bsp1720I GCTNAGC 1 cut(s) 1122
Bsp19I CCATGG 1 cut(s) 377
BspACI CCGC 4 cut(s) 638, 1252, 1317, 1607
BspANI GGCC 4 cut(s) 132, 690, 892, 897
BspCNI CTCAG 3 cut(s) 759, 1135, 1492
BspLI GGNNCC 3 cut(s) 270, 520, 1529
BspMAI CTGCAG 1 cut(s) 175
BspOI GCTAGC 1 cut(s) 1266
BspPI GGATC 2 cut(s) 1555, 1574
BspT104I TTCGAA 1 cut(s) 733
BspT107I GGYRCC 1 cut(s) 1527
BsrDI GCAATG 2 cut(s) 105, 1737
BsrGI TGTACA 1 cut(s) 1462
BsrI ACTGG 5 cut(s) 212, 353, 740, 1485, 1787
BssECI CCNNGG 8 cut(s) 265, 377, 1140, 1226, 1400, 1401, 1402, 1720
BssMI GATC 7 cut(s) 178, 607, 700, 925, 1547, 1579, 1792
BssSI CACGAG 1 cut(s) 957
BssT1I CCWWGG 1 cut(s) 377
Bst2BI CACGAG 1 cut(s) 957
Bst2UI CCWGG 8 cut(s) 423, 696, 894, 1142, 1335, 1638, 1677, 1721
Bst4CI ACNGT 8 cut(s) 250, 408, 511, 811, 832, 1213, 1325, 1770
Bst6I CTCTTC 2 cut(s) 806, 1721
BstAPI GCANNNNNTGC 1 cut(s) 1118
BstAUI TGTACA 1 cut(s) 1462
BstBAI YACGTR 2 cut(s) 949, 1540
BstBI TTCGAA 1 cut(s) 733
BstC8I GCNNGC 7 cut(s) 483, 636, 692, 940, 1083, 1264, 1314
BstDEI CTNAG 8 cut(s) 50, 154, 500, 767, 1122, 1431, 1500, 1702
BstDSI CCRYGG 1 cut(s) 377
BstEII GGTNACC 1 cut(s) 1413
BstENI CCTNNNNNAGG 1 cut(s) 1638
BstF5I GGATG 3 cut(s) 342, 1136, 1649
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 1 cut(s) 842
BstKTI GATC 7 cut(s) 181, 610, 703, 928, 1550, 1582, 1795
BstMAI GTCTC 1 cut(s) 670
BstMBI GATC 7 cut(s) 178, 607, 700, 925, 1547, 1579, 1792
BstMWI GCNNNNNNNGC 6 cut(s) 104, 848, 959, 980, 1078, 1118
BstNI CCWGG 8 cut(s) 423, 696, 894, 1142, 1335, 1638, 1677, 1721
BstNSI RCATGY 1 cut(s) 485
BstPAI GACNNNNGTC 1 cut(s) 1411
BstPI GGTNACC 1 cut(s) 1413
BstSFI CTRYAG 3 cut(s) 171, 1321, 1766
BstSLI GKGCMC 3 cut(s) 413, 470, 1401
BstV1I GCAGC 7 cut(s) 59, 157, 376, 860, 940, 1018, 1271
BstV2I GAAGAC 1 cut(s) 1333
BstX2I RGATCY 2 cut(s) 1579, 1792
BstXI CCANNNNNNTGG 3 cut(s) 353, 1644, 1683
BstYI RGATCY 2 cut(s) 1579, 1792
BsuRI GGCC 4 cut(s) 132, 690, 892, 897
BtgI CCRYGG 1 cut(s) 377
BtsCI GGATG 3 cut(s) 342, 1136, 1649
BtsI GCAGTG 2 cut(s) 1005, 1116
BtsIMutI CAGTG 7 cut(s) 219, 346, 413, 507, 1005, 1116, 1330
Cac8I GCNNGC 7 cut(s) 483, 636, 692, 940, 1083, 1264, 1314
CfoI GCGC 1 cut(s) 842
Cfr13I GGNCC 7 cut(s) 33, 269, 389, 890, 1238, 1504, 1760
Cfr9I CCCGGG 2 cut(s) 265, 1401
CseI GACGC 1 cut(s) 854
CsiI ACCWGGT 1 cut(s) 1333
Csp6I GTAC 4 cut(s) 1021, 1331, 1463, 1528
CviAII CATG 8 cut(s) 188, 353, 378, 482, 883, 1156, 1267, 1523
CviQI GTAC 4 cut(s) 1021, 1331, 1463, 1528
DdeI CTNAG 8 cut(s) 50, 154, 500, 767, 1122, 1431, 1500, 1702
DpnI GATC 7 cut(s) 180, 609, 702, 927, 1549, 1581, 1794
DpnII GATC 7 cut(s) 178, 607, 700, 925, 1547, 1579, 1792
DraIII CACNNNGTG 2 cut(s) 408, 474
EaeI YGGCCR 2 cut(s) 688, 895
Eam1104I CTCTTC 2 cut(s) 806, 1721
EarI CTCTTC 2 cut(s) 806, 1721
Eco130I CCWWGG 1 cut(s) 377
Eco24I GRGCYC 2 cut(s) 523, 1136
Eco47I GGWCC 6 cut(s) 33, 269, 389, 1238, 1504, 1760
Eco47III AGCGCT 1 cut(s) 841
Eco57I CTGAAG 2 cut(s) 380, 1460
Eco88I CYCGRG 2 cut(s) 265, 1401
Eco91I GGTNACC 1 cut(s) 1413
EcoNI CCTNNNNNAGG 1 cut(s) 1638
EcoO109I RGGNCCY 2 cut(s) 33, 890
EcoO65I GGTNACC 1 cut(s) 1413
EcoRI GAATTC 2 cut(s) 191, 735
EcoRII CCWGG 8 cut(s) 421, 694, 892, 1140, 1333, 1636, 1675, 1719
EcoT14I CCWWGG 1 cut(s) 377
EcoT22I ATGCAT 3 cut(s) 676, 1518, 1695
EcoT38I GRGCYC 2 cut(s) 523, 1136
ErhI CCWWGG 1 cut(s) 377
FaeI CATG 8 cut(s) 191, 356, 381, 485, 886, 1159, 1270, 1526
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 3 cut(s) 282, 1136, 1419
FatI CATG 8 cut(s) 187, 352, 377, 481, 882, 1155, 1266, 1522
FauI CCCGC 2 cut(s) 1259, 1614
FbaI TGATCA 1 cut(s) 925
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 9 cut(s) 73, 171, 365, 639, 849, 954, 1032, 1260, 1317
FokI GGATG 3 cut(s) 329, 1123, 1656
FriOI GRGCYC 2 cut(s) 523, 1136
Fsp4HI GCNGC 9 cut(s) 73, 171, 365, 639, 849, 954, 1032, 1260, 1317
FspBI CTAG 5 cut(s) 537, 887, 1074, 1263, 1742
GlaI GCGC 1 cut(s) 841
GluI GCNGC 9 cut(s) 73, 171, 365, 639, 849, 954, 1032, 1260, 1317
GsaI CCCAGC 1 cut(s) 938
GsuI CTGGAG 2 cut(s) 1620, 1770
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 4 cut(s) 132, 690, 892, 897
HapII CCGG 3 cut(s) 266, 1389, 1402
HgaI GACGC 1 cut(s) 854
HhaI GCGC 1 cut(s) 842
Hin1II CATG 8 cut(s) 191, 356, 381, 485, 886, 1159, 1270, 1526
Hin6I GCGC 1 cut(s) 840
HinP1I GCGC 1 cut(s) 840
HincII GTYRAC 2 cut(s) 778, 1603
HindII GTYRAC 2 cut(s) 778, 1603
HinfI GANTC 4 cut(s) 296, 746, 755, 771
HpaII CCGG 3 cut(s) 266, 1389, 1402
HphI GGTGA 5 cut(s) 22, 331, 710, 1336, 1407
Hpy166II GTNNAC 5 cut(s) 447, 514, 778, 1331, 1603
Hpy188I TCNGA 3 cut(s) 287, 670, 969
Hpy188III TCNNGA 6 cut(s) 392, 766, 794, 1478, 1551, 1747
Hpy8I GTNNAC 5 cut(s) 447, 514, 778, 1331, 1603
Hpy99I CGWCG 1 cut(s) 1425
HpyAV CCTTC 6 cut(s) 131, 143, 151, 568, 596, 997
HpyCH4III ACNGT 8 cut(s) 250, 408, 511, 811, 832, 1213, 1325, 1770
HpyCH4IV ACGT 3 cut(s) 399, 948, 1539
HpyF10VI GCNNNNNNNGC 6 cut(s) 104, 848, 959, 980, 1078, 1118
HpyF3I CTNAG 8 cut(s) 50, 154, 500, 767, 1122, 1431, 1500, 1702
HpySE526I ACGT 3 cut(s) 399, 948, 1539
Hsp92II CATG 8 cut(s) 191, 356, 381, 485, 886, 1159, 1270, 1526
HspAI GCGC 1 cut(s) 840
KpnI GGTACC 1 cut(s) 1531
Ksp22I TGATCA 1 cut(s) 925
Kzo9I GATC 7 cut(s) 178, 607, 700, 925, 1547, 1579, 1792
LmnI GCTCC 3 cut(s) 425, 518, 653
Lsp1109I GCAGC 7 cut(s) 59, 157, 376, 860, 940, 1018, 1271
LweI GCATC 6 cut(s) 661, 1444, 1525, 1680, 1696, 1793
MabI ACCWGGT 1 cut(s) 1333
MaeI CTAG 5 cut(s) 537, 887, 1074, 1263, 1742
MaeII ACGT 3 cut(s) 399, 948, 1539
MaeIII GTNAC 7 cut(s) 313, 400, 756, 916, 1413, 1492, 1697
MalI GATC 7 cut(s) 180, 609, 702, 927, 1549, 1581, 1794
MboI GATC 7 cut(s) 178, 607, 700, 925, 1547, 1579, 1792
MboII GAAGA 6 cut(s) 188, 581, 793, 1333, 1511, 1708
MflI RGATCY 2 cut(s) 1579, 1792
MhlI GDGCHC 6 cut(s) 413, 470, 523, 658, 1136, 1401
MlsI TGGCCA 2 cut(s) 690, 897
MluCI AATT 8 cut(s) 87, 191, 735, 1204, 1376, 1442, 1589, 1776
MluNI TGGCCA 2 cut(s) 690, 897
MlyI GAGTC 2 cut(s) 764, 780
Mox20I TGGCCA 2 cut(s) 690, 897
Mph1103I ATGCAT 3 cut(s) 676, 1518, 1695
MroXI GAANNNNTTC 1 cut(s) 729
MscI TGGCCA 2 cut(s) 690, 897
MseI TTAA 4 cut(s) 86, 684, 1307, 1662
MslI CAYNNNNRTG 4 cut(s) 186, 351, 1521, 1704
Msp20I TGGCCA 2 cut(s) 690, 897
MspI CCGG 3 cut(s) 266, 1389, 1402
Mva1269I GAATGC 3 cut(s) 985, 1033, 1112
MvaI CCWGG 8 cut(s) 423, 696, 894, 1142, 1335, 1638, 1677, 1721
MwoI GCNNNNNNNGC 6 cut(s) 104, 848, 959, 980, 1078, 1118
NciI CCSGG 4 cut(s) 266, 267, 1402, 1403
NcoI CCATGG 1 cut(s) 377
NdeII GATC 7 cut(s) 178, 607, 700, 925, 1547, 1579, 1792
NheI GCTAGC 1 cut(s) 1262
NlaIII CATG 8 cut(s) 191, 356, 381, 485, 886, 1159, 1270, 1526
NlaIV GGNNCC 3 cut(s) 270, 520, 1529
NmuCI GTSAC 5 cut(s) 400, 756, 1413, 1492, 1697
NsiI ATGCAT 3 cut(s) 676, 1518, 1695
NspI RCATGY 1 cut(s) 485
NspV TTCGAA 1 cut(s) 733
PaeI GCATGC 1 cut(s) 485
PctI GAATGC 3 cut(s) 985, 1033, 1112
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 2 cut(s) 296, 746
PflMI CCANNNNNTGG 1 cut(s) 348
PfoI TCCNGGA 1 cut(s) 1636
PkrI GCNGC 9 cut(s) 74, 172, 366, 640, 850, 955, 1033, 1261, 1318
PleI GAGTC 2 cut(s) 763, 779
PpsI GAGTC 2 cut(s) 763, 779
Ppu21I YACGTR 2 cut(s) 949, 1540
PpuMI RGGWCCY 1 cut(s) 33
PshAI GACNNNNGTC 1 cut(s) 1411
PshBI ATTAAT 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 33
Psp6I CCWGG 8 cut(s) 421, 694, 892, 1140, 1333, 1636, 1675, 1719
PspEI GGTNACC 1 cut(s) 1413
PspFI CCCAGC 1 cut(s) 934
PspGI CCWGG 8 cut(s) 421, 694, 892, 1140, 1333, 1636, 1675, 1719
PspN4I GGNNCC 3 cut(s) 270, 520, 1529
PspPI GGNCC 7 cut(s) 33, 269, 389, 890, 1238, 1504, 1760
PspPPI RGGWCCY 1 cut(s) 33
PstI CTGCAG 1 cut(s) 175
PsuI RGATCY 2 cut(s) 1579, 1792
RsaI GTAC 4 cut(s) 1022, 1332, 1464, 1529
RsaNI GTAC 4 cut(s) 1021, 1331, 1463, 1528
RseI CAYNNNNRTG 4 cut(s) 186, 351, 1521, 1704
SaqAI TTAA 4 cut(s) 86, 684, 1307, 1662
SatI GCNGC 9 cut(s) 73, 171, 365, 639, 849, 954, 1032, 1260, 1317
Sau3AI GATC 7 cut(s) 178, 607, 700, 925, 1547, 1579, 1792
Sau96I GGNCC 7 cut(s) 33, 269, 389, 890, 1238, 1504, 1760
SchI GAGTC 2 cut(s) 764, 780
SduI GDGCHC 6 cut(s) 413, 470, 523, 658, 1136, 1401
SexAI ACCWGGT 1 cut(s) 1333
SfaNI GCATC 6 cut(s) 661, 1444, 1525, 1680, 1696, 1793
SfcI CTRYAG 3 cut(s) 171, 1321, 1766
SfuI TTCGAA 1 cut(s) 733
SinI GGWCC 6 cut(s) 33, 269, 389, 1238, 1504, 1760
SmaI CCCGGG 2 cut(s) 267, 1403
SmiMI CAYNNNNRTG 4 cut(s) 186, 351, 1521, 1704
SmlI CTYRAG 1 cut(s) 1016
SmoI CTYRAG 1 cut(s) 1016
SphI GCATGC 1 cut(s) 485
Sse9I AATT 8 cut(s) 87, 191, 735, 1204, 1376, 1442, 1589, 1776
SsiI CCGC 4 cut(s) 638, 1252, 1317, 1607
SspMI CTAG 5 cut(s) 537, 887, 1074, 1263, 1742
StyI CCWWGG 1 cut(s) 377
TaaI ACNGT 8 cut(s) 250, 408, 511, 811, 832, 1213, 1325, 1770
TaiI ACGT 3 cut(s) 402, 951, 1542
TaqI TCGA 5 cut(s) 610, 733, 990, 1657, 1746
TasI AATT 8 cut(s) 87, 191, 735, 1204, 1376, 1442, 1589, 1776
TatI WGTACW 2 cut(s) 1020, 1462
TauI GCSGC 2 cut(s) 641, 1319
TfiI GAWTC 2 cut(s) 296, 746
Tru1I TTAA 4 cut(s) 86, 684, 1307, 1662
Tru9I TTAA 4 cut(s) 86, 684, 1307, 1662
TscAI CASTG 7 cut(s) 219, 353, 413, 514, 1012, 1123, 1330
TseFI GTSAC 5 cut(s) 400, 756, 1413, 1492, 1697
TseI GCWGC 7 cut(s) 72, 170, 364, 848, 953, 1031, 1259
Tsp45I GTSAC 5 cut(s) 400, 756, 1413, 1492, 1697
TspDTI ATGAA 5 cut(s) 184, 204, 1171, 1283, 1511
TspGWI ACGGA 4 cut(s) 289, 531, 1767, 1773
TspMI CCCGGG 2 cut(s) 265, 1401
TspRI CASTG 7 cut(s) 219, 353, 413, 514, 1012, 1123, 1330
Van91I CCANNNNNTGG 1 cut(s) 348
VpaK11BI GGWCC 6 cut(s) 33, 269, 389, 1238, 1504, 1760
VspI ATTAAT 1 cut(s) 684
XagI CCTNNNNNAGG 1 cut(s) 1638
XapI RAATTY 3 cut(s) 191, 735, 1589
XceI RCATGY 1 cut(s) 485
XmaI CCCGGG 2 cut(s) 265, 1401
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 5 cut(s) 537, 887, 1074, 1263, 1742
Zsp2I ATGCAT 3 cut(s) 676, 1518, 1695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.