MD11G1058200.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016555
Physical Location & Seq
Reverse (-)
4945438 .. 4947558
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1058200.v1.1.491

Sequence Viewer

Length: 1791 bp
ATGGAGCCCTCCAAACTTACCCTTCTGCTTGGCCTTCTTGCCTTCCTTACTTTCTTAGCTTCCTTCTCTGCTGCAGAAGATCACTTCCATGAATTCTTCGTTGAAGCGAGGCCGGTGAAGAGGCTGTGCAGAACCCACAGCAGAATCACAGTCAATGGCCAGTTCCCAGGACCAACTTTGGAAGTCAGAAATGGAGATTCCGTTGCCGTCAAAGTTACCAACAGAGAGAAATACAACGTCACCATCCACTGGCATGGACGGAAGCAGCAGAACAATCCATGGGAAGAAGGACCCGAATATGTCACACAGTGCCCTATCCAACCAGGAGCTACCTACACATACAGGTTCACAATCACAGACCAAGAGGGCACGTTGTGGTGGCATGCTCACAGCAGATGGCTCAGAGCCAGTGTCTATGGAGCCCTCATAATCTACCCTAGATTGGGTTCTCCCTACCCCTTCCCGATGCCACAACGAGAAGTTCCCCTTCTTCTTGGGGAATGGTTTGATCGAAACCCCCAAGATGTCATGAGGCTTGCGGCTTTCACAGGAGGAGCACCAAATGTCTCTGATGCATACACCATTAATGGACAGCCTGGTGATCTTTACAGATGTTCCAAAAAAGAAACCATTCGAATTCCAGTTGTATCGGGCGAGTCACTTCTCCTGAGAGTGATCAACTCAGCACTCAATCAAGAACTCTTCTTTACCGTTGCCAACCACCAACTGACTGTTGTGGGCGCTGACGCATCTTACACAGAACCTCTCACAACCAGAGTTATCATGATAGGCCCTGGCCAAACAACCGATGTTCTGCTTACTGCTGATCAGCGCCCTGCTCGGTACTACATGGCTGCTCGTGCCTATCAGACTGCCCAGAATGTGCCCTTCGACAATACCACCACCACTGCTATCCTTGAGTACAAGAATGCTGCTTCTTCAAATCAAGGTTTAGCACCAGTATTTCCTACATTGCCAGCTTACAACGACACAGCAACTGCTACTGCATACACTGCTCAGATAAAGAGCCCATCCCAGGTCAAAGTGCCCACACAGATTGACGAGAACCTGTTTTTCACTGTGGGTTTAGGACCTTATAACTGCACGGTTCCCAACAGCCCTTGGTGTCAAGGACCAAACAACACCCGCTTTGCAGCTAGCATGAACAATGTTTCCTTTGCATTACCAAGTACCAACTCCTCAATGCAAGCTGCCTACTATGGTGTCCTCGGTGTCTTCACCACAGACTTTCCACCTGTTCCTCTTGTGCAATTCAATTACACCGGAAATGTGCCCAGGGGGCTATGGTCACCTTGTCGTGGAACTAAGCTCTACAAGTTGAAGTACGGTGCTAATGTACAAATAGTGTTTCAGGACACAAGTACTGTGACTATTGATGACCATCCTATGCATTTTCATGGATGCCATTCCTACGTGATTGGATCAGGATTTGGAAACTACAACCCCAGCACGGATCCAGCGAGATTCAACCTCATTGACCCGCCACAGAGAAATACCATCGGAACACCTCCAGGGGGATGGGTAGCTATACGATTTAAGGCTGATAATCCAGGTAACATGCTAATCTCGTATCCTAAGCTTTCATCTAACCAATATTTGTTCACTATTTTGATATCTTTCTACTTCTTGACCCAATTAGGCGTCTGGCTGATGCACTGTCACTTAGATTCACATCTCTTCCTGGGTTTGGCTATGGCTTGTCTAGTCGAGAACGGAATCGGACCATTACAGACTGTAATTCCTCCTCCACCAGATCTACCCCGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

597

Amino Acids

66.21

Weight (kDa)

7.97

Isoelectric Point (pI)

34.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 36 - 147 1.6e-41 Multicopper oxidase
Cu-oxidase PF00394 160 - 311 3.8e-42 Multicopper oxidase
Cu-oxidase_2 PF07731 417 - 527 1.3e-25 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30210
fragaria_vesca FvH4_3g39750
malus_domestica MD03G1056400.v1.1 MD11G1057600.v1.1 MD11G1058200.v1.1
pyrus_communis pycom03g04470 pycom11g04820
rosa_chinensis RchiOBHm_Chr5g0071631
rosa_laevigata RLG00000036231 RLG00000036234
rosa_multiflora Rmu_sc0005745.1_g000031
rosa_rugosa Rorug05G0413700
rosa_samantha Rh5CG513000
rosa_wichuraiana Rw5G043640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1098
AccB7I CCANNNNNTGG 1 cut(s) 249
AciI CCGC 3 cut(s) 539, 1147, 1502
AclWI GGATC 3 cut(s) 1450, 1469, 1482
AcoI YGGCCR 2 cut(s) 157, 796
AcsI RAATTY 2 cut(s) 92, 636
AcyI GRCGYC 1 cut(s) 1662
AdeI CACNNNGTG 2 cut(s) 309, 375
AfaI GTAC 6 cut(s) 845, 923, 1192, 1346, 1359, 1384
AfiI CCNNNNNNNGG 8 cut(s) 249, 442, 443, 1036, 1319, 1471, 1535, 1708
AgsI TTSAA 5 cut(s) 104, 942, 1276, 1342, 1489
AjnI CCWGG 9 cut(s) 166, 322, 595, 793, 1035, 1295, 1531, 1570, 1701
AloI GAACNNNNNNTCC 2 cut(s) 1157, 1189
AluBI AGCT 8 cut(s) 59, 329, 980, 1157, 1211, 1330, 1547, 1600
AluI AGCT 8 cut(s) 59, 329, 980, 1157, 1211, 1330, 1547, 1600
Alw21I GWGCWC 1 cut(s) 559
Alw26I GTCTC 1 cut(s) 571
AlwI GGATC 3 cut(s) 1450, 1469, 1482
AlwNI CAGNNNCTG 1 cut(s) 998
AoxI GGCC 5 cut(s) 31, 110, 157, 790, 796
ApeKI GCWGC 6 cut(s) 71, 265, 854, 932, 1154, 1211
ApoI RAATTY 2 cut(s) 92, 636
AseI ATTAAT 1 cut(s) 585
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 2 cut(s) 743, 834
AspS9I GGNCC 6 cut(s) 170, 290, 791, 1091, 1133, 1742
AsuHPI GGTGA 5 cut(s) 127, 232, 611, 1231, 1302
AsuII TTCGAA 1 cut(s) 634
AsuNHI GCTAGC 1 cut(s) 1157
AvaII GGWCC 5 cut(s) 170, 290, 1091, 1133, 1742
BaeGI GKGCMC 5 cut(s) 314, 371, 888, 1050, 1296
BalI TGGCCA 2 cut(s) 159, 798
BamHI GGATCC 1 cut(s) 1474
BanII GRGCYC 3 cut(s) 9, 424, 1031
BauI CACGAG 1 cut(s) 858
BbsI GAAGAC 1 cut(s) 1228
Bbv12I GWGCWC 1 cut(s) 559
BbvI GCAGC 6 cut(s) 58, 277, 841, 919, 1166, 1198
BccI CCATC 6 cut(s) 251, 390, 1039, 1410, 1526, 1533
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 9 cut(s) 168, 324, 597, 795, 1037, 1297, 1533, 1572, 1703
BciVI GTATCC 1 cut(s) 1602
BclI TGATCA 2 cut(s) 675, 826
BcoDI GTCTC 1 cut(s) 571
BfaI CTAG 3 cut(s) 438, 1158, 1724
BfmI CTRYAG 1 cut(s) 72
BfoI RGCGCY 2 cut(s) 744, 835
BfuI GTATCC 1 cut(s) 1602
BglI GCCNNNNNGGC 1 cut(s) 1300
BglII AGATCT 1 cut(s) 1774
BisI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
BlsI GCNGC 7 cut(s) 73, 267, 541, 856, 934, 1156, 1213
BmcAI AGTACT 1 cut(s) 1384
Bme1390I CCNGG 9 cut(s) 168, 324, 597, 795, 1037, 1297, 1533, 1572, 1703
Bme18I GGWCC 5 cut(s) 170, 290, 1091, 1133, 1742
BmgT120I GGNCC 6 cut(s) 170, 290, 791, 1091, 1133, 1742
BmiI GGNNCC 5 cut(s) 6, 292, 421, 1110, 1476
BmrFI CCNGG 9 cut(s) 168, 324, 597, 795, 1037, 1297, 1533, 1572, 1703
BmsI GCATC 6 cut(s) 456, 562, 758, 1412, 1662, 1775
BmtI GCTAGC 1 cut(s) 1161
BpiI GAAGAC 1 cut(s) 1228
BpmI CTGGAG 1 cut(s) 1515
Bpu10I CCTNAGC 1 cut(s) 1596
Bpu14I TTCGAA 1 cut(s) 634
BpuEI CTTGAG 1 cut(s) 938
BsaAI YACGTR 1 cut(s) 1435
BsaBI GATNNNNATC 2 cut(s) 1401, 1692
BsaHI GRCGYC 1 cut(s) 1662
BsaWI WCCGGW 1 cut(s) 1283
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 8 cut(s) 249, 442, 443, 1036, 1319, 1471, 1535, 1708
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 5 cut(s) 160, 254, 408, 641, 959
Bse3DI GCAATG 1 cut(s) 971
Bse8I GATNNNNATC 2 cut(s) 1401, 1692
BseBI CCWGG 9 cut(s) 168, 324, 597, 795, 1037, 1297, 1533, 1572, 1703
BseGI GGATG 5 cut(s) 243, 1031, 1402, 1427, 1544
BseJI GATNNNNATC 2 cut(s) 1401, 1692
BseLI CCNNNNNNNGG 8 cut(s) 249, 442, 443, 1036, 1319, 1471, 1535, 1708
BseMI GCAATG 1 cut(s) 971
BseMII CTCAG 4 cut(s) 415, 659, 696, 1031
BseNI ACTGG 5 cut(s) 160, 254, 408, 641, 959
BseRI GAGGAG 3 cut(s) 567, 1189, 1755
BseSI GKGCMC 5 cut(s) 314, 371, 888, 1050, 1296
BseXI GCAGC 6 cut(s) 58, 277, 841, 919, 1166, 1198
BseYI CCCAGC 1 cut(s) 1466
BsgI GTGCAG 2 cut(s) 148, 1087
BshFI GGCC 5 cut(s) 33, 112, 159, 792, 798
BsiHKAI GWGCWC 1 cut(s) 559
BsiSI CCGG 2 cut(s) 113, 1284
BslI CCNNNNNNNGG 8 cut(s) 249, 442, 443, 1036, 1319, 1471, 1535, 1708
BsmAI GTCTC 1 cut(s) 571
BsmI GAATGC 1 cut(s) 934
BsnI GGCC 5 cut(s) 33, 112, 159, 792, 798
Bsp119I TTCGAA 1 cut(s) 634
Bsp1286I GDGCHC 9 cut(s) 9, 314, 371, 424, 559, 888, 1031, 1050, 1296
Bsp1407I TGTACA 1 cut(s) 1357
Bsp143I GATC 8 cut(s) 79, 508, 601, 675, 826, 1442, 1474, 1774
Bsp19I CCATGG 1 cut(s) 278
BspACI CCGC 3 cut(s) 539, 1147, 1502
BspANI GGCC 5 cut(s) 33, 112, 159, 792, 798
BspCNI CTCAG 4 cut(s) 414, 660, 695, 1030
BspHI TCATGA 2 cut(s) 528, 783
BspLI GGNNCC 5 cut(s) 6, 292, 421, 1110, 1476
BspMAI CTGCAG 1 cut(s) 76
BspOI GCTAGC 1 cut(s) 1161
BspPI GGATC 3 cut(s) 1450, 1469, 1482
BspT104I TTCGAA 1 cut(s) 634
BsrDI GCAATG 1 cut(s) 971
BsrFI RCCGGY 1 cut(s) 112
BsrGI TGTACA 1 cut(s) 1357
BsrI ACTGG 5 cut(s) 160, 254, 408, 641, 959
BssAI RCCGGY 1 cut(s) 112
BssMI GATC 8 cut(s) 79, 508, 601, 675, 826, 1442, 1474, 1774
BssNI GRCGYC 1 cut(s) 1662
BssSI CACGAG 1 cut(s) 858
BssT1I CCWWGG 2 cut(s) 278, 1121
Bst2BI CACGAG 1 cut(s) 858
Bst2UI CCWGG 9 cut(s) 168, 324, 597, 795, 1037, 1297, 1533, 1572, 1703
Bst6I CTCTTC 3 cut(s) 113, 707, 1703
BstACI GRCGYC 1 cut(s) 1662
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstAUI TGTACA 1 cut(s) 1357
BstBAI YACGTR 1 cut(s) 1435
BstBI TTCGAA 1 cut(s) 634
BstC8I GCNNGC 5 cut(s) 384, 537, 978, 1159, 1209
BstDEI CTNAG 8 cut(s) 55, 401, 668, 682, 1017, 1326, 1596, 1684
BstDSI CCRYGG 1 cut(s) 278
BstEII GGTNACC 1 cut(s) 1308
BstF5I GGATG 5 cut(s) 243, 1031, 1402, 1427, 1544
BstH2I RGCGCY 2 cut(s) 744, 835
BstHHI GCGC 2 cut(s) 743, 834
BstKTI GATC 8 cut(s) 82, 511, 604, 678, 829, 1445, 1477, 1777
BstMAI GTCTC 1 cut(s) 571
BstMBI GATC 8 cut(s) 79, 508, 601, 675, 826, 1442, 1474, 1774
BstMWI GCNNNNNNNGC 3 cut(s) 860, 1013, 1300
BstNI CCWGG 9 cut(s) 168, 324, 597, 795, 1037, 1297, 1533, 1572, 1703
BstNSI RCATGY 2 cut(s) 386, 1582
BstPI GGTNACC 1 cut(s) 1308
BstSCI CCNGG 9 cut(s) 166, 322, 595, 793, 1035, 1295, 1531, 1570, 1701
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 5 cut(s) 314, 371, 888, 1050, 1296
BstV1I GCAGC 6 cut(s) 58, 277, 841, 919, 1166, 1198
BstV2I GAAGAC 1 cut(s) 1228
BstX2I RGATCY 2 cut(s) 1474, 1774
BstXI CCANNNNNNTGG 2 cut(s) 254, 1539
BstYI RGATCY 2 cut(s) 1474, 1774
BsuI GTATCC 1 cut(s) 1602
BsuRI GGCC 5 cut(s) 33, 112, 159, 792, 798
BtgI CCRYGG 1 cut(s) 278
BtsCI GGATG 5 cut(s) 243, 1031, 1402, 1427, 1544
BtsI GCAGTG 2 cut(s) 906, 1011
BtsIMutI CAGTG 7 cut(s) 247, 314, 415, 906, 1011, 1077, 1675
Cac8I GCNNGC 5 cut(s) 384, 537, 978, 1159, 1209
CaiI CAGNNNCTG 1 cut(s) 998
CciI TCATGA 2 cut(s) 528, 783
CfoI GCGC 2 cut(s) 743, 834
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 6 cut(s) 170, 290, 791, 1091, 1133, 1742
CseI GACGC 2 cut(s) 755, 1651
Csp6I GTAC 6 cut(s) 844, 922, 1191, 1345, 1358, 1383
CspCI CAANNNNNGTGG 2 cut(s) 1039, 1074
CviQI GTAC 6 cut(s) 844, 922, 1191, 1345, 1358, 1383
DdeI CTNAG 8 cut(s) 55, 401, 668, 682, 1017, 1326, 1596, 1684
DpnI GATC 8 cut(s) 81, 510, 603, 677, 828, 1444, 1476, 1776
DpnII GATC 8 cut(s) 79, 508, 601, 675, 826, 1442, 1474, 1774
DraIII CACNNNGTG 2 cut(s) 309, 375
EaeI YGGCCR 2 cut(s) 157, 796
Eam1104I CTCTTC 3 cut(s) 113, 707, 1703
EarI CTCTTC 3 cut(s) 113, 707, 1703
Eco130I CCWWGG 2 cut(s) 278, 1121
Eco24I GRGCYC 3 cut(s) 9, 424, 1031
Eco32I GATATC 1 cut(s) 1635
Eco47I GGWCC 5 cut(s) 170, 290, 1091, 1133, 1742
Eco91I GGTNACC 1 cut(s) 1308
EcoO109I RGGNCCY 3 cut(s) 290, 791, 1091
EcoO65I GGTNACC 1 cut(s) 1308
EcoRI GAATTC 2 cut(s) 92, 636
EcoRII CCWGG 9 cut(s) 166, 322, 595, 793, 1035, 1295, 1531, 1570, 1701
EcoRV GATATC 1 cut(s) 1635
EcoT14I CCWWGG 2 cut(s) 278, 1121
EcoT22I ATGCAT 2 cut(s) 577, 1413
EcoT38I GRGCYC 3 cut(s) 9, 424, 1031
ErhI CCWWGG 2 cut(s) 278, 1121
FalI AAGNNNNNCTT 2 cut(s) 471, 503
FauI CCCGC 2 cut(s) 1154, 1509
FbaI TGATCA 2 cut(s) 675, 826
Fnu4HI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
FokI GGATG 5 cut(s) 230, 1018, 1389, 1434, 1551
FriOI GRGCYC 3 cut(s) 9, 424, 1031
Fsp4HI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
FspBI CTAG 3 cut(s) 438, 1158, 1724
GlaI GCGC 2 cut(s) 742, 833
GluI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
GsaI CCCAGC 1 cut(s) 1470
GsuI CTGGAG 1 cut(s) 1515
HaeII RGCGCY 2 cut(s) 744, 835
HaeIII GGCC 5 cut(s) 33, 112, 159, 792, 798
HapII CCGG 2 cut(s) 113, 1284
HgaI GACGC 2 cut(s) 755, 1651
HhaI GCGC 2 cut(s) 743, 834
Hin1I GRCGYC 1 cut(s) 1662
Hin6I GCGC 2 cut(s) 741, 832
HinP1I GCGC 2 cut(s) 741, 832
HindIII AAGCTT 1 cut(s) 1598
HinfI GANTC 6 cut(s) 144, 197, 656, 1485, 1688, 1737
HpaII CCGG 2 cut(s) 113, 1284
HphI GGTGA 5 cut(s) 127, 232, 611, 1231, 1302
Hpy166II GTNNAC 2 cut(s) 348, 1623
Hpy188I TCNGA 7 cut(s) 188, 404, 571, 870, 1020, 1523, 1742
Hpy188III TCNNGA 9 cut(s) 463, 529, 667, 695, 784, 1373, 1446, 1648, 1729
Hpy8I GTNNAC 2 cut(s) 348, 1623
HpyAV CCTTC 8 cut(s) 32, 44, 52, 73, 281, 469, 497, 898
HpyCH4IV ACGT 3 cut(s) 237, 371, 1434
HpyF10VI GCNNNNNNNGC 3 cut(s) 860, 1013, 1300
HpyF3I CTNAG 8 cut(s) 55, 401, 668, 682, 1017, 1326, 1596, 1684
HpySE526I ACGT 3 cut(s) 237, 371, 1434
Hsp92I GRCGYC 1 cut(s) 1662
HspAI GCGC 2 cut(s) 741, 832
Ksp22I TGATCA 2 cut(s) 675, 826
Kzo9I GATC 8 cut(s) 79, 508, 601, 675, 826, 1442, 1474, 1774
LmnI GCTCC 4 cut(s) 4, 326, 419, 554
Lsp1109I GCAGC 6 cut(s) 58, 277, 841, 919, 1166, 1198
LweI GCATC 6 cut(s) 456, 562, 758, 1412, 1662, 1775
MaeI CTAG 3 cut(s) 438, 1158, 1724
MaeII ACGT 3 cut(s) 237, 371, 1434
MaeIII GTNAC 8 cut(s) 214, 238, 301, 657, 1308, 1387, 1574, 1679
MalI GATC 8 cut(s) 81, 510, 603, 677, 828, 1444, 1476, 1776
MboI GATC 8 cut(s) 79, 508, 601, 675, 826, 1442, 1474, 1774
MboII GAAGA 9 cut(s) 88, 89, 130, 296, 482, 694, 930, 1228, 1690
MflI RGATCY 2 cut(s) 1474, 1774
MhlI GDGCHC 9 cut(s) 9, 314, 371, 424, 559, 888, 1031, 1050, 1296
MlsI TGGCCA 2 cut(s) 159, 798
MluCI AATT 6 cut(s) 92, 636, 1271, 1276, 1655, 1758
MluNI TGGCCA 2 cut(s) 159, 798
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 1 cut(s) 343
Mox20I TGGCCA 2 cut(s) 159, 798
Mph1103I ATGCAT 2 cut(s) 577, 1413
MroXI GAANNNNTTC 1 cut(s) 630
MscI TGGCCA 2 cut(s) 159, 798
MseI TTAA 2 cut(s) 585, 1557
MslI CAYNNNNRTG 3 cut(s) 87, 252, 1416
Msp20I TGGCCA 2 cut(s) 159, 798
MspI CCGG 2 cut(s) 113, 1284
MspR9I CCNGG 9 cut(s) 168, 324, 597, 795, 1037, 1297, 1533, 1572, 1703
Mva1269I GAATGC 1 cut(s) 934
MvaI CCWGG 9 cut(s) 168, 324, 597, 795, 1037, 1297, 1533, 1572, 1703
MwoI GCNNNNNNNGC 3 cut(s) 860, 1013, 1300
NcoI CCATGG 1 cut(s) 278
NdeII GATC 8 cut(s) 79, 508, 601, 675, 826, 1442, 1474, 1774
NheI GCTAGC 1 cut(s) 1157
NlaIV GGNNCC 5 cut(s) 6, 292, 421, 1110, 1476
NmuCI GTSAC 6 cut(s) 238, 301, 657, 1308, 1387, 1679
NsiI ATGCAT 2 cut(s) 577, 1413
NspI RCATGY 2 cut(s) 386, 1582
NspV TTCGAA 1 cut(s) 634
PaeI GCATGC 1 cut(s) 386
PagI TCATGA 2 cut(s) 528, 783
PasI CCCWGGG 1 cut(s) 1296
PcsI WCGNNNNNNNCGW 1 cut(s) 1478
PctI GAATGC 1 cut(s) 934
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 5 cut(s) 144, 197, 1485, 1688, 1737
PflMI CCANNNNNTGG 1 cut(s) 249
PkrI GCNGC 7 cut(s) 73, 267, 541, 856, 934, 1156, 1213
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
Ppu21I YACGTR 1 cut(s) 1435
PpuMI RGGWCCY 2 cut(s) 290, 1091
PshBI ATTAAT 1 cut(s) 585
PsiI TTATAA 1 cut(s) 1098
Psp5II RGGWCCY 2 cut(s) 290, 1091
Psp6I CCWGG 9 cut(s) 166, 322, 595, 793, 1035, 1295, 1531, 1570, 1701
PspEI GGTNACC 1 cut(s) 1308
PspFI CCCAGC 1 cut(s) 1466
PspGI CCWGG 9 cut(s) 166, 322, 595, 793, 1035, 1295, 1531, 1570, 1701
PspN4I GGNNCC 5 cut(s) 6, 292, 421, 1110, 1476
PspPI GGNCC 6 cut(s) 170, 290, 791, 1091, 1133, 1742
PspPPI RGGWCCY 2 cut(s) 290, 1091
PstI CTGCAG 1 cut(s) 76
PstNI CAGNNNCTG 1 cut(s) 998
PsuI RGATCY 2 cut(s) 1474, 1774
RsaI GTAC 6 cut(s) 845, 923, 1192, 1346, 1359, 1384
RsaNI GTAC 6 cut(s) 844, 922, 1191, 1345, 1358, 1383
RseI CAYNNNNRTG 3 cut(s) 87, 252, 1416
SaqAI TTAA 2 cut(s) 585, 1557
SatI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
Sau3AI GATC 8 cut(s) 79, 508, 601, 675, 826, 1442, 1474, 1774
Sau96I GGNCC 6 cut(s) 170, 290, 791, 1091, 1133, 1742
ScaI AGTACT 1 cut(s) 1384
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 9 cut(s) 168, 324, 597, 795, 1037, 1297, 1533, 1572, 1703
SduI GDGCHC 9 cut(s) 9, 314, 371, 424, 559, 888, 1031, 1050, 1296
SfaNI GCATC 6 cut(s) 456, 562, 758, 1412, 1662, 1775
SfcI CTRYAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 634
SinI GGWCC 5 cut(s) 170, 290, 1091, 1133, 1742
SmiMI CAYNNNNRTG 3 cut(s) 87, 252, 1416
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
SphI GCATGC 1 cut(s) 386
Sse9I AATT 6 cut(s) 92, 636, 1271, 1276, 1655, 1758
SsiI CCGC 3 cut(s) 539, 1147, 1502
SspI AATATT 1 cut(s) 1616
SspMI CTAG 3 cut(s) 438, 1158, 1724
StyD4I CCNGG 9 cut(s) 166, 322, 595, 793, 1035, 1295, 1531, 1570, 1701
StyI CCWWGG 2 cut(s) 278, 1121
TaiI ACGT 3 cut(s) 240, 374, 1437
TaqI TCGA 4 cut(s) 511, 634, 891, 1728
TasI AATT 6 cut(s) 92, 636, 1271, 1276, 1655, 1758
TatI WGTACW 3 cut(s) 921, 1357, 1382
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 5 cut(s) 144, 197, 1485, 1688, 1737
Tru1I TTAA 2 cut(s) 585, 1557
Tru9I TTAA 2 cut(s) 585, 1557
TscAI CASTG 7 cut(s) 254, 314, 415, 913, 1018, 1084, 1682
TseFI GTSAC 6 cut(s) 238, 301, 657, 1308, 1387, 1679
TseI GCWGC 6 cut(s) 71, 265, 854, 932, 1154, 1211
Tsp45I GTSAC 6 cut(s) 238, 301, 657, 1308, 1387, 1679
TspDTI ATGAA 4 cut(s) 105, 1178, 1406, 1593
TspGWI ACGGA 4 cut(s) 190, 274, 1487, 1749
TspRI CASTG 7 cut(s) 254, 314, 415, 913, 1018, 1084, 1682
Van91I CCANNNNNTGG 1 cut(s) 249
VpaK11BI GGWCC 5 cut(s) 170, 290, 1091, 1133, 1742
VspI ATTAAT 1 cut(s) 585
XapI RAATTY 2 cut(s) 92, 636
XceI RCATGY 2 cut(s) 386, 1582
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 3 cut(s) 438, 1158, 1724
ZrmI AGTACT 1 cut(s) 1384
Zsp2I ATGCAT 2 cut(s) 577, 1413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.