MD11G1057600.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016555
Physical Location & Seq
Reverse (-)
4883457 .. 4885803
2347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1057600.v1.1.491

Sequence Viewer

Length: 1704 bp
ATGGAGGCCTCCAAACTTACCCTTCTGCTTGGCCTTCTTGCCTTGCTCACTTTCTTAGCTTCCTTCTCAGCTGCAGAAGTTCACTTCTATGAATTCATCGTTGAAGCGAGGCCGGTCATGAGGCTGTGCAGAACCCTCAGTACAATCACAGTCAATGGCCAGTTCCCAGGTCCAACTTTGGAAGTCAGAAATGGAGATTCCGTTGCCGTCAAAGTTACCAACAGAGCAAGATACAACATCACCATCCACTGGCATGGACTGAAGCAGCAGAACAATCCATGGGCAGATGGTCCGGAATACGTCACACAGTGCCCTATCCAACCAGGAGCTACCTACACATACAGGTTCACAATCAAAGACCAAGAGGGCACTTTGTGGTGGCATGCTCACAGCAGATGGCTCAGAGCCACTGTCTATGGAGCCCTCATAATCTACCCTAGATTGGGTTCTCCCTACCCCTTCCCGATGCCAAAACGAGAAGTTCCCATTCTTCTTGGGGAATGGTTTGATCGAGACCCCCAAGATGTCATGAGGCTGGCGGCTTTCACAGGAGGAGCACCAAATGTCTCTGATGCATACACCATTAATGGCCAGCCTGGTGATCTTTACAGATGTTCCAGACAAGAAACCATTCGAATTCCAGTCGTATCTGGCGAGTCACTTCTCCTGAGAGTCATCAACTCTGCACTCAATCAAGAACTCTTCTTTACCGTTGCCAACCACCAACTGACTGTTGTGGGCGCTGACGCATCTTACACAAGACCTTTCACAACCAGAGTTATCATGATAGGCCCTGGCCAAACAACCGATGTTCTGCTTACTGCTGATCAGCGCCCTGCTCGGTACTACATGGCTGCTCGTGCCTATGCGACTGCCCAGAATGCGCCCTTCGACAATACCACCACCACTGCTATCCTTGAGTACAAGAATGCTGCTTCTTCAAATCAAGGTTTAGCACCAGTATTTCCTACATTGCCAGCTTACAACGATACAGCAACTGCTACTGCATACACTGCTCAGATAAAGAGCCCATCCCAGGTCAGAGTCCCCACGCAGATTGACGAGTACCTGTTTTTCACTGTGGGTTTAGGACTTTATAACTGCACAGTTCCCAACAGCCCTCGGTGTCAAGGACCAAACAACACCCGCTTTGCAGCCAGCATGAACAATGTTTCCTTTGTATTACCAAGAACCAACTCCTTAATGCAAGCTGCCTACTATGGTGTACCCGGTGTCTTCACCACAGACTTTCCACCTATTCCTCCTGTGCAATTCAATTATACCGGAAATGTGCCCCGGGGGCTATGGTCACCTCGTCGTGGAACTAAGCTTTACAGGTTGAAGTATTGTGCTAATGTACAAATAGTGCTTCAGGACACAAGTATAGTGACTACTGAGGACCATCCTATGCATCTTCATGGGTACCATTTCTATGTGATTGGATCAGGATTTGGAAACTACAACCCCAGCACAGATCCAGCGAGATTCAACCTCATCGACCCGCCACAGAGAAATACCATCGGAACACCTCCAGGGGGATGGGTAGCTATACGATTTAAGGCTGATAATCCAGGTGTCTGGCTGATGCACTGTCACTTAGATTCACATCTCTTCCTGGGTTTGGCTATGGCTTTTCTAGTCGAGAACGGAATTGGACCATTACAGTCTGTAATTCCCCCTCCACCAGATCTACCCCGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

568

Amino Acids

62.96

Weight (kDa)

8.77

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 38 - 147 6.6e-45 Multicopper oxidase
Cu-oxidase PF00394 160 - 311 4.1e-42 Multicopper oxidase
Cu-oxidase_2 PF07731 416 - 549 3.8e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016555)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30210
fragaria_vesca FvH4_3g39750
malus_domestica MD03G1056400.v1.1 MD11G1057600.v1.1 MD11G1058200.v1.1
pyrus_communis pycom03g04470 pycom11g04820
rosa_chinensis RchiOBHm_Chr5g0071631
rosa_laevigata RLG00000036231 RLG00000036234
rosa_multiflora Rmu_sc0005745.1_g000031
rosa_rugosa Rorug05G0413700
rosa_samantha Rh5CG513000
rosa_wichuraiana Rw5G043640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1098
Acc65I GGTACC 1 cut(s) 1422
AccB1I GGYRCC 1 cut(s) 1422
AccB7I CCANNNNNTGG 1 cut(s) 249
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 3 cut(s) 539, 1147, 1502
AclWI GGATC 2 cut(s) 1450, 1469
AcoI YGGCCR 3 cut(s) 157, 589, 796
AcsI RAATTY 2 cut(s) 92, 636
AcuI CTGAAG 2 cut(s) 281, 1355
AdeI CACNNNGTG 2 cut(s) 309, 375
AfaI GTAC 7 cut(s) 142, 845, 923, 1067, 1227, 1359, 1424
AfiI CCNNNNNNNGG 7 cut(s) 249, 442, 443, 1036, 1319, 1535, 1621
AgsI TTSAA 5 cut(s) 104, 942, 1276, 1342, 1489
AjnI CCWGG 8 cut(s) 166, 322, 595, 793, 1035, 1531, 1570, 1614
AloI GAACNNNNNNTCC 2 cut(s) 1157, 1189
AluBI AGCT 7 cut(s) 59, 71, 329, 980, 1211, 1330, 1547
AluI AGCT 7 cut(s) 59, 71, 329, 980, 1211, 1330, 1547
Alw21I GWGCWC 1 cut(s) 559
Alw26I GTCTC 2 cut(s) 507, 571
AlwI GGATC 2 cut(s) 1450, 1469
AlwNI CAGNNNCTG 1 cut(s) 998
Ama87I CYCGRG 1 cut(s) 1296
Aor13HI TCCGGA 1 cut(s) 292
AoxI GGCC 7 cut(s) 6, 31, 110, 157, 589, 790, 796
ApeKI GCWGC 6 cut(s) 71, 265, 854, 932, 1154, 1211
ApoI RAATTY 2 cut(s) 92, 636
AseI ATTAAT 1 cut(s) 585
Asp700I GAANNNNTTC 1 cut(s) 630
Asp718I GGTACC 1 cut(s) 1422
AspLEI GCGC 3 cut(s) 743, 834, 886
AspS9I GGNCC 6 cut(s) 170, 290, 791, 1133, 1399, 1655
AsuC2I CCSGG 3 cut(s) 1230, 1297, 1298
AsuHPI GGTGA 4 cut(s) 232, 611, 1231, 1302
AsuII TTCGAA 1 cut(s) 634
AvaI CYCGRG 1 cut(s) 1296
AvaII GGWCC 5 cut(s) 170, 290, 1133, 1399, 1655
BaeGI GKGCMC 3 cut(s) 314, 371, 1296
BalI TGGCCA 3 cut(s) 159, 591, 798
BanI GGYRCC 1 cut(s) 1422
BanII GRGCYC 2 cut(s) 424, 1031
BauI CACGAG 1 cut(s) 858
BbsI GAAGAC 1 cut(s) 1228
Bbv12I GWGCWC 1 cut(s) 559
BbvI GCAGC 6 cut(s) 58, 277, 841, 919, 1166, 1198
BccI CCATC 7 cut(s) 251, 281, 390, 1039, 1410, 1526, 1533
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 8 cut(s) 168, 324, 597, 795, 1037, 1533, 1572, 1616
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 3 cut(s) 1230, 1297, 1298
BcoDI GTCTC 2 cut(s) 507, 571
BfaI CTAG 2 cut(s) 438, 1637
BfmI CTRYAG 1 cut(s) 72
BfoI RGCGCY 2 cut(s) 744, 835
BglI GCCNNNNNGGC 1 cut(s) 1300
BglII AGATCT 1 cut(s) 1687
BisI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
BlsI GCNGC 7 cut(s) 73, 267, 541, 856, 934, 1156, 1213
Bme18I GGWCC 5 cut(s) 170, 290, 1133, 1399, 1655
BmeT110I CYCGRG 1 cut(s) 1296
BmgT120I GGNCC 6 cut(s) 170, 290, 791, 1133, 1399, 1655
BmiI GGNNCC 2 cut(s) 421, 1424
BmsI GCATC 6 cut(s) 456, 562, 758, 1420, 1575, 1688
BpiI GAAGAC 1 cut(s) 1228
BpmI CTGGAG 1 cut(s) 1515
Bpu14I TTCGAA 1 cut(s) 634
BpuEI CTTGAG 1 cut(s) 938
BpuMI CCSGG 3 cut(s) 1230, 1297, 1298
BsaBI GATNNNNATC 1 cut(s) 1605
BsaI GGTCTC 1 cut(s) 507
BsaWI WCCGGW 2 cut(s) 292, 1283
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 7 cut(s) 249, 442, 443, 1036, 1319, 1535, 1621
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 4 cut(s) 160, 254, 641, 959
Bse3DI GCAATG 1 cut(s) 971
Bse8I GATNNNNATC 1 cut(s) 1605
BseAI TCCGGA 1 cut(s) 292
BseBI CCWGG 8 cut(s) 168, 324, 597, 795, 1037, 1533, 1572, 1616
BseGI GGATG 4 cut(s) 243, 1031, 1402, 1544
BseJI GATNNNNATC 1 cut(s) 1605
BseLI CCNNNNNNNGG 7 cut(s) 249, 442, 443, 1036, 1319, 1535, 1621
BseMI GCAATG 1 cut(s) 971
BseMII CTCAG 6 cut(s) 81, 151, 415, 659, 1031, 1386
BseNI ACTGG 4 cut(s) 160, 254, 641, 959
BseRI GAGGAG 1 cut(s) 567
BseSI GKGCMC 3 cut(s) 314, 371, 1296
BseXI GCAGC 6 cut(s) 58, 277, 841, 919, 1166, 1198
BseYI CCCAGC 1 cut(s) 1466
BsgI GTGCAG 3 cut(s) 148, 669, 1087
BshFI GGCC 7 cut(s) 8, 33, 112, 159, 591, 792, 798
BshNI GGYRCC 1 cut(s) 1422
BsiHKAI GWGCWC 1 cut(s) 559
BsiHKCI CYCGRG 1 cut(s) 1296
BsiSI CCGG 5 cut(s) 113, 293, 1230, 1284, 1297
BslFI GGGAC 1 cut(s) 1031
BslI CCNNNNNNNGG 7 cut(s) 249, 442, 443, 1036, 1319, 1535, 1621
BsmAI GTCTC 2 cut(s) 507, 571
BsmFI GGGAC 1 cut(s) 1031
BsmI GAATGC 2 cut(s) 886, 934
BsnI GGCC 7 cut(s) 8, 33, 112, 159, 591, 792, 798
Bso31I GGTCTC 1 cut(s) 507
BsoBI CYCGRG 1 cut(s) 1296
Bsp119I TTCGAA 1 cut(s) 634
Bsp1286I GDGCHC 6 cut(s) 314, 371, 424, 559, 1031, 1296
Bsp13I TCCGGA 1 cut(s) 292
Bsp1407I TGTACA 1 cut(s) 1357
Bsp143I GATC 6 cut(s) 508, 601, 826, 1442, 1474, 1687
Bsp19I CCATGG 1 cut(s) 278
BspACI CCGC 3 cut(s) 539, 1147, 1502
BspANI GGCC 7 cut(s) 8, 33, 112, 159, 591, 792, 798
BspCNI CTCAG 6 cut(s) 80, 150, 414, 660, 1030, 1387
BspEI TCCGGA 1 cut(s) 292
BspHI TCATGA 3 cut(s) 117, 528, 783
BspLI GGNNCC 2 cut(s) 421, 1424
BspMAI CTGCAG 1 cut(s) 76
BspPI GGATC 2 cut(s) 1450, 1469
BspT104I TTCGAA 1 cut(s) 634
BspT107I GGYRCC 1 cut(s) 1422
BspTNI GGTCTC 1 cut(s) 507
BsrDI GCAATG 1 cut(s) 971
BsrFI RCCGGY 1 cut(s) 112
BsrGI TGTACA 1 cut(s) 1357
BsrI ACTGG 4 cut(s) 160, 254, 641, 959
BssAI RCCGGY 1 cut(s) 112
BssMI GATC 6 cut(s) 508, 601, 826, 1442, 1474, 1687
BssSI CACGAG 1 cut(s) 858
BssT1I CCWWGG 1 cut(s) 278
Bst2BI CACGAG 1 cut(s) 858
Bst2UI CCWGG 8 cut(s) 168, 324, 597, 795, 1037, 1533, 1572, 1616
Bst4CI ACNGT 9 cut(s) 151, 309, 412, 712, 733, 1081, 1108, 1592, 1665
Bst6I CTCTTC 2 cut(s) 707, 1616
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstAUI TGTACA 1 cut(s) 1357
BstBI TTCGAA 1 cut(s) 634
BstC8I GCNNGC 6 cut(s) 384, 537, 593, 978, 1159, 1209
BstDEI CTNAG 9 cut(s) 55, 67, 137, 401, 668, 1017, 1326, 1395, 1597
BstDSI CCRYGG 1 cut(s) 278
BstEII GGTNACC 1 cut(s) 1308
BstF5I GGATG 4 cut(s) 243, 1031, 1402, 1544
BstH2I RGCGCY 2 cut(s) 744, 835
BstHHI GCGC 3 cut(s) 743, 834, 886
BstKTI GATC 6 cut(s) 511, 604, 829, 1445, 1477, 1690
BstMAI GTCTC 2 cut(s) 507, 571
BstMBI GATC 6 cut(s) 508, 601, 826, 1442, 1474, 1687
BstMWI GCNNNNNNNGC 4 cut(s) 860, 881, 1013, 1300
BstNI CCWGG 8 cut(s) 168, 324, 597, 795, 1037, 1533, 1572, 1616
BstNSI RCATGY 1 cut(s) 386
BstPI GGTNACC 1 cut(s) 1308
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 3 cut(s) 314, 371, 1296
BstV1I GCAGC 6 cut(s) 58, 277, 841, 919, 1166, 1198
BstV2I GAAGAC 1 cut(s) 1228
BstX2I RGATCY 2 cut(s) 1474, 1687
BstXI CCANNNNNNTGG 3 cut(s) 254, 1539, 1578
BstYI RGATCY 2 cut(s) 1474, 1687
BsuRI GGCC 7 cut(s) 8, 33, 112, 159, 591, 792, 798
BtgI CCRYGG 1 cut(s) 278
BtsCI GGATG 4 cut(s) 243, 1031, 1402, 1544
BtsI GCAGTG 2 cut(s) 906, 1011
BtsIMutI CAGTG 7 cut(s) 247, 314, 408, 906, 1011, 1077, 1588
Cac8I GCNNGC 6 cut(s) 384, 537, 593, 978, 1159, 1209
CaiI CAGNNNCTG 1 cut(s) 998
CciI TCATGA 3 cut(s) 117, 528, 783
CfoI GCGC 3 cut(s) 743, 834, 886
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 6 cut(s) 170, 290, 791, 1133, 1399, 1655
Cfr9I CCCGGG 1 cut(s) 1296
CseI GACGC 1 cut(s) 755
Csp6I GTAC 7 cut(s) 141, 844, 922, 1066, 1226, 1358, 1423
CspCI CAANNNNNGTGG 2 cut(s) 1039, 1074
CviAII CATG 9 cut(s) 118, 254, 279, 383, 529, 784, 850, 1162, 1418
CviQI GTAC 7 cut(s) 141, 844, 922, 1066, 1226, 1358, 1423
DdeI CTNAG 9 cut(s) 55, 67, 137, 401, 668, 1017, 1326, 1395, 1597
DpnI GATC 6 cut(s) 510, 603, 828, 1444, 1476, 1689
DpnII GATC 6 cut(s) 508, 601, 826, 1442, 1474, 1687
DraIII CACNNNGTG 2 cut(s) 309, 375
EaeI YGGCCR 3 cut(s) 157, 589, 796
Eam1104I CTCTTC 2 cut(s) 707, 1616
EarI CTCTTC 2 cut(s) 707, 1616
Eco130I CCWWGG 1 cut(s) 278
Eco147I AGGCCT 1 cut(s) 8
Eco24I GRGCYC 2 cut(s) 424, 1031
Eco31I GGTCTC 1 cut(s) 507
Eco47I GGWCC 5 cut(s) 170, 290, 1133, 1399, 1655
Eco57I CTGAAG 2 cut(s) 281, 1355
Eco88I CYCGRG 1 cut(s) 1296
Eco91I GGTNACC 1 cut(s) 1308
EcoO109I RGGNCCY 1 cut(s) 791
EcoO65I GGTNACC 1 cut(s) 1308
EcoRI GAATTC 2 cut(s) 92, 636
EcoRII CCWGG 8 cut(s) 166, 322, 595, 793, 1035, 1531, 1570, 1614
EcoT14I CCWWGG 1 cut(s) 278
EcoT22I ATGCAT 2 cut(s) 577, 1413
EcoT38I GRGCYC 2 cut(s) 424, 1031
ErhI CCWWGG 1 cut(s) 278
FaeI CATG 9 cut(s) 121, 257, 282, 386, 532, 787, 853, 1165, 1421
FaqI GGGAC 1 cut(s) 1031
FatI CATG 9 cut(s) 117, 253, 278, 382, 528, 783, 849, 1161, 1417
FauI CCCGC 2 cut(s) 1154, 1509
FbaI TGATCA 1 cut(s) 826
Fnu4HI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
FokI GGATG 4 cut(s) 230, 1018, 1389, 1551
FriOI GRGCYC 2 cut(s) 424, 1031
Fsp4HI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
FspBI CTAG 2 cut(s) 438, 1637
GlaI GCGC 3 cut(s) 742, 833, 885
GluI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
GsaI CCCAGC 1 cut(s) 1470
GsuI CTGGAG 1 cut(s) 1515
HaeII RGCGCY 2 cut(s) 744, 835
HaeIII GGCC 7 cut(s) 8, 33, 112, 159, 591, 792, 798
HapII CCGG 5 cut(s) 113, 293, 1230, 1284, 1297
HgaI GACGC 1 cut(s) 755
HhaI GCGC 3 cut(s) 743, 834, 886
Hin1II CATG 9 cut(s) 121, 257, 282, 386, 532, 787, 853, 1165, 1421
Hin6I GCGC 3 cut(s) 741, 832, 884
HinP1I GCGC 3 cut(s) 741, 832, 884
HindIII AAGCTT 1 cut(s) 1328
HinfI GANTC 6 cut(s) 197, 656, 672, 1044, 1485, 1601
HpaII CCGG 5 cut(s) 113, 293, 1230, 1284, 1297
HphI GGTGA 4 cut(s) 232, 611, 1231, 1302
Hpy166II GTNNAC 3 cut(s) 82, 348, 1226
Hpy188I TCNGA 6 cut(s) 188, 404, 571, 1020, 1043, 1523
Hpy8I GTNNAC 3 cut(s) 82, 348, 1226
Hpy99I CGWCG 1 cut(s) 1320
HpyAV CCTTC 5 cut(s) 32, 44, 73, 469, 898
HpyCH4III ACNGT 9 cut(s) 151, 309, 412, 712, 733, 1081, 1108, 1592, 1665
HpyCH4IV ACGT 1 cut(s) 300
HpyF10VI GCNNNNNNNGC 4 cut(s) 860, 881, 1013, 1300
HpyF3I CTNAG 9 cut(s) 55, 67, 137, 401, 668, 1017, 1326, 1395, 1597
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 9 cut(s) 121, 257, 282, 386, 532, 787, 853, 1165, 1421
HspAI GCGC 3 cut(s) 741, 832, 884
Kpn2I TCCGGA 1 cut(s) 292
KpnI GGTACC 1 cut(s) 1426
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 6 cut(s) 508, 601, 826, 1442, 1474, 1687
LmnI GCTCC 3 cut(s) 326, 419, 554
Lsp1109I GCAGC 6 cut(s) 58, 277, 841, 919, 1166, 1198
LweI GCATC 6 cut(s) 456, 562, 758, 1420, 1575, 1688
MaeI CTAG 2 cut(s) 438, 1637
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 6 cut(s) 214, 301, 657, 1308, 1387, 1592
MalI GATC 6 cut(s) 510, 603, 828, 1444, 1476, 1689
MboI GATC 6 cut(s) 508, 601, 826, 1442, 1474, 1687
MboII GAAGA 6 cut(s) 482, 694, 930, 1228, 1406, 1603
MflI RGATCY 2 cut(s) 1474, 1687
MhlI GDGCHC 6 cut(s) 314, 371, 424, 559, 1031, 1296
MlsI TGGCCA 3 cut(s) 159, 591, 798
MluCI AATT 6 cut(s) 92, 636, 1271, 1276, 1650, 1671
MluNI TGGCCA 3 cut(s) 159, 591, 798
MlyI GAGTC 3 cut(s) 665, 681, 1053
MmeI TCCRAC 2 cut(s) 197, 343
Mox20I TGGCCA 3 cut(s) 159, 591, 798
Mph1103I ATGCAT 2 cut(s) 577, 1413
MroI TCCGGA 1 cut(s) 292
MroXI GAANNNNTTC 1 cut(s) 630
MscI TGGCCA 3 cut(s) 159, 591, 798
MseI TTAA 3 cut(s) 585, 1202, 1557
MslI CAYNNNNRTG 4 cut(s) 87, 252, 1416, 1431
Msp20I TGGCCA 3 cut(s) 159, 591, 798
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 5 cut(s) 113, 293, 1230, 1284, 1297
Mva1269I GAATGC 2 cut(s) 886, 934
MvaI CCWGG 8 cut(s) 168, 324, 597, 795, 1037, 1533, 1572, 1616
MwoI GCNNNNNNNGC 4 cut(s) 860, 881, 1013, 1300
NciI CCSGG 3 cut(s) 1230, 1297, 1298
NcoI CCATGG 1 cut(s) 278
NdeII GATC 6 cut(s) 508, 601, 826, 1442, 1474, 1687
NlaIII CATG 9 cut(s) 121, 257, 282, 386, 532, 787, 853, 1165, 1421
NlaIV GGNNCC 2 cut(s) 421, 1424
NmuCI GTSAC 5 cut(s) 301, 657, 1308, 1387, 1592
NsiI ATGCAT 2 cut(s) 577, 1413
NspI RCATGY 1 cut(s) 386
NspV TTCGAA 1 cut(s) 634
PaeI GCATGC 1 cut(s) 386
PagI TCATGA 3 cut(s) 117, 528, 783
PceI AGGCCT 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 651
PctI GAATGC 2 cut(s) 886, 934
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 3 cut(s) 197, 1485, 1601
PflMI CCANNNNNTGG 1 cut(s) 249
PkrI GCNGC 7 cut(s) 73, 267, 541, 856, 934, 1156, 1213
PleI GAGTC 3 cut(s) 664, 680, 1052
PpsI GAGTC 3 cut(s) 664, 680, 1052
PshBI ATTAAT 1 cut(s) 585
PsiI TTATAA 1 cut(s) 1098
Psp6I CCWGG 8 cut(s) 166, 322, 595, 793, 1035, 1531, 1570, 1614
PspEI GGTNACC 1 cut(s) 1308
PspFI CCCAGC 1 cut(s) 1466
PspGI CCWGG 8 cut(s) 166, 322, 595, 793, 1035, 1531, 1570, 1614
PspN4I GGNNCC 2 cut(s) 421, 1424
PspPI GGNCC 6 cut(s) 170, 290, 791, 1133, 1399, 1655
PsrI GAACNNNNNNTAC 2 cut(s) 124, 156
PstI CTGCAG 1 cut(s) 76
PstNI CAGNNNCTG 1 cut(s) 998
PsuI RGATCY 2 cut(s) 1474, 1687
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 7 cut(s) 142, 845, 923, 1067, 1227, 1359, 1424
RsaNI GTAC 7 cut(s) 141, 844, 922, 1066, 1226, 1358, 1423
RseI CAYNNNNRTG 4 cut(s) 87, 252, 1416, 1431
SaqAI TTAA 3 cut(s) 585, 1202, 1557
SatI GCNGC 7 cut(s) 72, 266, 540, 855, 933, 1155, 1212
Sau3AI GATC 6 cut(s) 508, 601, 826, 1442, 1474, 1687
Sau96I GGNCC 6 cut(s) 170, 290, 791, 1133, 1399, 1655
SchI GAGTC 3 cut(s) 665, 681, 1053
SduI GDGCHC 6 cut(s) 314, 371, 424, 559, 1031, 1296
SfaNI GCATC 6 cut(s) 456, 562, 758, 1420, 1575, 1688
SfcI CTRYAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 634
SinI GGWCC 5 cut(s) 170, 290, 1133, 1399, 1655
SmaI CCCGGG 1 cut(s) 1298
SmiMI CAYNNNNRTG 4 cut(s) 87, 252, 1416, 1431
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
SphI GCATGC 1 cut(s) 386
Sse9I AATT 6 cut(s) 92, 636, 1271, 1276, 1650, 1671
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 3 cut(s) 539, 1147, 1502
SspMI CTAG 2 cut(s) 438, 1637
StuI AGGCCT 1 cut(s) 8
StyI CCWWGG 1 cut(s) 278
TaaI ACNGT 9 cut(s) 151, 309, 412, 712, 733, 1081, 1108, 1592, 1665
TaiI ACGT 1 cut(s) 303
TaqI TCGA 5 cut(s) 511, 634, 891, 1497, 1641
TasI AATT 6 cut(s) 92, 636, 1271, 1276, 1650, 1671
TatI WGTACW 3 cut(s) 140, 921, 1357
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 3 cut(s) 197, 1485, 1601
Tru1I TTAA 3 cut(s) 585, 1202, 1557
Tru9I TTAA 3 cut(s) 585, 1202, 1557
TscAI CASTG 7 cut(s) 254, 314, 415, 913, 1018, 1084, 1595
TseFI GTSAC 5 cut(s) 301, 657, 1308, 1387, 1592
TseI GCWGC 6 cut(s) 71, 265, 854, 932, 1154, 1211
Tsp45I GTSAC 5 cut(s) 301, 657, 1308, 1387, 1592
TspDTI ATGAA 4 cut(s) 85, 105, 1178, 1406
TspGWI ACGGA 2 cut(s) 190, 1662
TspMI CCCGGG 1 cut(s) 1296
TspRI CASTG 7 cut(s) 254, 314, 415, 913, 1018, 1084, 1595
Van91I CCANNNNNTGG 1 cut(s) 249
VpaK11BI GGWCC 5 cut(s) 170, 290, 1133, 1399, 1655
VspI ATTAAT 1 cut(s) 585
XapI RAATTY 2 cut(s) 92, 636
XceI RCATGY 1 cut(s) 386
XmaI CCCGGG 1 cut(s) 1296
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 2 cut(s) 438, 1637
Zsp2I ATGCAT 2 cut(s) 577, 1413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.