Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008410

Physical Location & Seq

Genomic Coordinates Forward (+)

6302515 .. 6309247

Gene Structure 6733 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1077800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2541 bp

ATGGCGCCTACTGCGAAATCGAACGGGAAAACCATCACAGACTCCGCCATTTCGTCCAAGATTCCGGCAACCGCAAACCCTTTAGCCGAAGAGCCGTCGCAGATTGCTTCCAACATCAATTACCACGCCAAATTCAGCCCCCACTTCTCGCCGTTCAAGTTCGAGCCGGAGCAAGCCTACTACGCCACCGCCGACAGCGTCCGCGATCGCCTGATCCAACAATGGAACGAGACGTACCTGCATTTCCACAAGGAGAATCCCAAGCAGACTTACTACTTGTCCATGGAGTATCTTCAAGGACGAGCCTTGACCAATGCGATTGGGAATTTGAATGTTCAGAGTGCTTATGCTGATGCTTTGAACAAGTTGGGGCATCAGCTGGAGGAAATAACAGAGCAGGAGAAAGATGCGGCACTTGGAAATGGTGGGCTGGGAAGGCTTGCTTCGTGCTTTCTAGACTCCATGGCAACATTGAATTTGCCTGCATGGGGGTATGGTTTGAGGTACAGGTACGGGCTGTTCAAGCAGCGGATTGCCGAGGATGGCCAGGAAGAAACTGCTGAGGATTGGCTAGAGAAATTTAGTCCTTGGGAAGTTGTCAGGCATGATGTTATGTACCCTGTCAGATTTTTTGGCCGTGTTCATGTCAATCCTGATGGATCCCGAAAGTGGGTTGAGGGAGAGGTTTTGCAAGCTCTGGCTTATGATGTGCCAATTCCAGGATACAAAACCAAGAACACTATTAGTCTCCGTCTATGGGAAGCCAAAGCATCTGCTGAGGATTTTAACCTATTTCAGTTTAATGATGGACAGTATGAATCTGCTGCGCAGCTTCATTCTCGAGCGCAACAGATTTGTGCTGTTCTATATCCTGGAGATGCCACAGAGGAGGGGAAACTTTTAAGGCTGAAGCAACAATTTTTCCTCTGCAGTGCATCACTTCAGGACATTATTTTTAGATTCAAGGAGAGGAAACAGGGGACAGACACATTGCATTGGTCAGAGTTCCCAAAAAAGGTTGCTGTCCAAATGAACGATACTCATCCTACACTGGCAATTCCGGAGCTGATGCGACTGCTATTGGATGAGGAAGGTCTTGGGTGGGATGAGGCATGGGAAGTGACAACAAGGACGGTTGCTTATACTAATCACACAGTCCTTCCTGAAGCACTTGAGAAATGGTCACAAGCCGTAATGTGGAAGCTTCTCCCTCGCCATATGCAAATAATAGAAGAAATTGACAAAAGATTCATTGCTATGGTACGTGCTACACGAAGTGATCTTGAGAGCAAAATTCCCAGCTTGTGCGTCTTGGATAACAATCCCCAAAAGCCTGTAGTGCGAATGGCAAATTTATGTGTGGTGTCTGCACATACGGTTAATGGTGTTGCCCAATTGCACAGTGATATTTTAAAAAATGAGTTATTTGTAGACTATGTCTCTATATGGCCAACAAAGTTCCAAAACAAAACCAATGGGATTACTCCTCGCCGGTGGCTTCGGTTTTGCAGTCCTGAGCTCAGTAATATAATCACCAAATGGTTAAAAACGGATGAATGGGTGACCAACCTGGACCTACTTGTAGGTCTTCGAAAGTTTGCTGATGATTCAAAATTACAAGAAGAATGGGCGTCTGCCAAGATGGCCAATAAGAAGCGTTTGGTGCAGTACATAGAGCATGTGGCAGGTGTCAAGATTGACCCAAACACTCTATTTGACATACAAGTGAAGCGTATCCATGAATATAAGAGACAGTTGCTGAATATTCTGGGCGCAGTTTATCGGTACAAGAAGTTAAAGGAGATGAGCCCTGAAGATCGGAAGAAGACAACTCCACGCACTATCATGATTGGAGGAAAAGCATTTGCAACATATACAAATGCAAAAAGAATAGTCAAGCTGGTAGATGATGTCGGTGCTGTGGTCAACAATGATCCTGATATCAATAGTTACTTGAAGGTTGTATTTGTTCCAAACTACAATGTATCTGTGGCCGAGATACTTATCCCAGGAAGTGAGCTGTCACAACATATCAGTACTGCAGGCATGGAGGCAAGTGGCACGAGCAACATGAAATTTGCTCTGAACGGCTGCCTCATAATCGGCACACTGGATGGGGCTAATGTGGAAATCCGTGAGGAAATTGGGGATGACAACTTTTTTCTCTTTGGTGCAACAGCTGATGAAGTCCCTAAGCTGCGCAAGGACAGAGAGAACGGCCTGTTCAAACCCGATCCTCGGTTTGAAGAAGCCAAGCAGTTTGTTAGGAGTGGAGCATTTGGGAGCTACGATTACAATCCACTTCTTGACTCTCTGGAGGGGAACACTGGTTATGGTCGTGGTGATTATTTTCTTGTTGGCCATGACTTTGCACAGTATGTGGATGCTCAGGCAAAAGTAGATGAAGCTTACAAGGATAGGAAAAGGTGGCTGAAGATGTCCATACTAAGCACTGCAGGGTCTGGCAAGTTCAGCAGCGACCGGACAATTGCTCAGTACGCCAAGGAAATCTGGAACATAGAGGAGTGCCGCGTACCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004645 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005977 GO:0005980 GO:0006073 GO:0006091 GO:0006112 GO:0006950 GO:0008144 GO:0008150 GO:0008152 GO:0008184 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010035 GO:0010038 GO:0015980 GO:0016052 GO:0016740 GO:0016757 GO:0016758 GO:0019842 GO:0030170 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048037 GO:0050662 GO:0050896 GO:0055114 GO:0070279 GO:0071704 GO:0097159 GO:1901363 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 ko04217 ko04910 ko04922 ko04931 map00500 map01100 map01110 map02026 map04217 map04910 map04922 map04931

Pfam Domains

Protein Families

Phosphorylase

Protein Analysis

847

Amino Acids

96.16

Weight (kDa)

6.36

Isoelectric Point (pI)

39.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphorylase	PF00343	120 - 840	9.7e-301	Carbohydrate phosphorylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46970
fragaria_vesca	FvH4_3g37910 FvH4_3g37910 FvH4_3g37910 FvH4_3g37910 FvH4_3g37910
malus_domestica	MD03G1077800.v1.1 MD05G1167500.v1.1 MD11G1082200.v1.1
prunus_persica	Prupe.6G062600_v2.0.a1
pyrus_communis	pycom03g06170 pycom11g07050
rosa_chinensis	RchiOBHm_Chr5g0068321
rosa_laevigata	RLG00000035965
rosa_multiflora	Rmu_sc0001418.1_g000022
rosa_roxburghii	Rroxscaffold_1G00012440 Rroxscaffold_1G00013180
rosa_rugosa	Rorug05G0388800
rosa_samantha	Rh5AG447300 Rh5BG465400 Rh5CG487000 Rh5DG479000
rosa_wichuraiana	Rw5G041770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1676
Acc16I	TGCGCA	2 cut(s)	828, 2201
Acc36I	ACCTGC	2 cut(s)	246, 1676
AccB1I	GGYRCC	1 cut(s)	4
AccI	GTMKAC	1 cut(s)	1431
AccII	CGCG	2 cut(s)	204, 2532
AccIII	TCCGGA	1 cut(s)	1060
AciI	CCGC	7 cut(s)	45, 72, 189, 202, 410, 529, 2530
AclWI	GGATC	5 cut(s)	208, 654, 667, 1928, 2228
AcoI	YGGCCR	6 cut(s)	544, 634, 1448, 1644, 1992, 2359
AcsI	RAATTY	7 cut(s)	131, 325, 475, 578, 1293, 1351, 2075
AcuI	CTGAAG	5 cut(s)	926, 929, 1185, 1833, 2453
AcyI	GRCGYC	2 cut(s)	5, 1631
AdeI	CACNNNGTG	1 cut(s)	1277
AfiI	CCNNNNNNNGG	8 cut(s)	488, 669, 670, 719, 757, 1015, 1197, 2238
AjnI	CCWGG	5 cut(s)	546, 718, 871, 1569, 2008
Alw21I	GWGCWC	1 cut(s)	1521
Alw26I	GTCTC	4 cut(s)	224, 752, 1444, 1744
AlwI	GGATC	5 cut(s)	208, 654, 667, 1928, 2228
AlwNI	CAGNNNCTG	2 cut(s)	1759, 2462
Ama87I	CYCGRG	1 cut(s)	840
Aor13HI	TCCGGA	1 cut(s)	1060
AoxI	GGCC	7 cut(s)	544, 634, 1448, 1644, 1992, 2218, 2359
ApeKI	GCWGC	6 cut(s)	526, 824, 829, 2091, 2197, 2475
ApoI	RAATTY	7 cut(s)	131, 325, 475, 578, 1293, 1351, 2075
AsiSI	GCGATCGC	1 cut(s)	208
AspLEI	GCGC	5 cut(s)	7, 829, 847, 1775, 2202
AspS9I	GGNCC	1 cut(s)	1573
AsuHPI	GGTGA	3 cut(s)	1525, 1573, 2354
AsuII	TTCGAA	1 cut(s)	1591
AvaI	CYCGRG	1 cut(s)	840
AvaII	GGWCC	1 cut(s)	1573
BalI	TGGCCA	4 cut(s)	546, 1450, 1646, 2361
BamHI	GGATCC	1 cut(s)	659
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	2 cut(s)	1521, 1811
BauI	CACGAG	1 cut(s)	2062
BbsI	GAAGAC	2 cut(s)	1580, 1832
Bbv12I	GWGCWC	1 cut(s)	1521
BbvCI	CCTCAGC	2 cut(s)	561, 777
BbvI	GCAGC	6 cut(s)	538, 811, 841, 2078, 2184, 2487
BccI	CCATC	6 cut(s)	41, 536, 650, 800, 1636, 2108
BceAI	ACGGC	6 cut(s)	79, 136, 621, 1175, 2104, 2233
BciT130I	CCWGG	5 cut(s)	548, 720, 873, 1571, 2010
BciVI	GTATCC	2 cut(s)	716, 1745
BcoDI	GTCTC	4 cut(s)	224, 752, 1444, 1744
BfaI	CTAG	2 cut(s)	455, 572
BfmI	CTRYAG	4 cut(s)	928, 1335, 2040, 2454
BfoI	RGCGCY	1 cut(s)	8
BfuAI	ACCTGC	2 cut(s)	246, 1676
BfuI	GTATCC	2 cut(s)	716, 1745
BglI	GCCNNNNNGGC	1 cut(s)	1643
BisI	GCNGC	8 cut(s)	411, 527, 825, 830, 2092, 2198, 2476, 2530
BlsI	GCNGC	8 cut(s)	412, 528, 826, 831, 2093, 2199, 2477, 2531
BmcAI	AGTACT	1 cut(s)	2038
Bme1390I	CCNGG	5 cut(s)	548, 720, 873, 1571, 2010
Bme18I	GGWCC	1 cut(s)	1573
BmeT110I	CYCGRG	1 cut(s)	840
BmgT120I	GGNCC	1 cut(s)	1573
BmiI	GGNNCC	2 cut(s)	6, 661
BmrFI	CCNGG	5 cut(s)	548, 720, 873, 1571, 2010
BmsI	GCATC	8 cut(s)	343, 382, 397, 779, 868, 944, 1059, 2374
BpiI	GAAGAC	2 cut(s)	1580, 1832
BpmI	CTGGAG	3 cut(s)	401, 894, 2336
Bpu10I	CCTNAGC	5 cut(s)	561, 777, 1515, 2193, 2388
Bpu14I	TTCGAA	1 cut(s)	1591
BpuEI	CTTGAG	2 cut(s)	1193, 1304
BsaAI	YACGTR	1 cut(s)	1265
BsaBI	GATNNNNATC	4 cut(s)	1041, 1320, 2003, 2295
BsaHI	GRCGYC	2 cut(s)	5, 1631
BsaJI	CCNNGG	7 cut(s)	282, 462, 537, 587, 2008, 2237, 2502
BsaWI	WCCGGW	2 cut(s)	1060, 2481
Bsc4I	CCNNNNNNNGG	8 cut(s)	488, 669, 670, 719, 757, 1015, 1197, 2238
Bse118I	RCCGGY	1 cut(s)	1491
Bse1I	ACTGG	3 cut(s)	1056, 2115, 2332
Bse3DI	GCAATG	2 cut(s)	989, 1251
Bse8I	GATNNNNATC	4 cut(s)	1041, 1320, 2003, 2295
BseAI	TCCGGA	1 cut(s)	1060
BseBI	CCWGG	5 cut(s)	548, 720, 873, 1571, 2010
BseDI	CCNNGG	7 cut(s)	282, 462, 537, 587, 2008, 2237, 2502
BseGI	GGATG	8 cut(s)	547, 1042, 1090, 1111, 1558, 2119, 2155, 2389
BseJI	GATNNNNATC	4 cut(s)	1041, 1320, 2003, 2295
BseLI	CCNNNNNNNGG	8 cut(s)	488, 669, 670, 719, 757, 1015, 1197, 2238
BseMI	GCAATG	2 cut(s)	989, 1251
BseMII	CTCAG	6 cut(s)	552, 768, 1506, 1534, 2402, 2507
BseNI	ACTGG	3 cut(s)	1056, 2115, 2332
BseRI	GAGGAG	3 cut(s)	902, 1476, 2537
BseXI	GCAGC	6 cut(s)	538, 811, 841, 2078, 2184, 2487
BseYI	CCCAGC	2 cut(s)	430, 1298
BsgI	GTGCAG	2 cut(s)	1353, 1685
Bsh1236I	CGCG	2 cut(s)	204, 2532
Bsh1285I	CGRYCG	2 cut(s)	208, 2482
BshFI	GGCC	7 cut(s)	546, 636, 1450, 1646, 1994, 2220, 2361
BshNI	GGYRCC	1 cut(s)	4
BsiEI	CGRYCG	2 cut(s)	208, 2482
BsiHKAI	GWGCWC	1 cut(s)	1521
BsiHKCI	CYCGRG	1 cut(s)	840
BsiSI	CCGG	5 cut(s)	65, 167, 1061, 1492, 2482
BslFI	GGGAC	2 cut(s)	994, 2174
BslI	CCNNNNNNNGG	8 cut(s)	488, 669, 670, 719, 757, 1015, 1197, 2238
BsmAI	GTCTC	4 cut(s)	224, 752, 1444, 1744
BsmBI	CGTCTC	1 cut(s)	224
BsmFI	GGGAC	2 cut(s)	994, 2174
BsnI	GGCC	7 cut(s)	546, 636, 1450, 1646, 1994, 2220, 2361
BsoBI	CYCGRG	1 cut(s)	840
Bsp119I	TTCGAA	1 cut(s)	1591
Bsp1286I	GDGCHC	2 cut(s)	1521, 1811
Bsp13I	TCCGGA	1 cut(s)	1060
Bsp143I	GATC	7 cut(s)	205, 213, 659, 1279, 1816, 1933, 2233
Bsp19I	CCATGG	2 cut(s)	282, 462
BspACI	CCGC	7 cut(s)	45, 72, 189, 202, 410, 529, 2530
BspANI	GGCC	7 cut(s)	546, 636, 1450, 1646, 1994, 2220, 2361
BspCNI	CTCAG	6 cut(s)	553, 769, 1507, 1533, 2401, 2506
BspEI	TCCGGA	1 cut(s)	1060
BspFNI	CGCG	2 cut(s)	204, 2532
BspHI	TCATGA	1 cut(s)	1845
BspLI	GGNNCC	2 cut(s)	6, 661
BspMAI	CTGCAG	3 cut(s)	932, 2044, 2458
BspMI	ACCTGC	2 cut(s)	246, 1676
BspPI	GGATC	5 cut(s)	208, 654, 667, 1928, 2228
BspQI	GCTCTTC	1 cut(s)	84
BspT104I	TTCGAA	1 cut(s)	1591
BspT107I	GGYRCC	1 cut(s)	4
BsrDI	GCAATG	2 cut(s)	989, 1251
BsrFI	RCCGGY	1 cut(s)	1491
BsrI	ACTGG	3 cut(s)	1056, 2115, 2332
BssAI	RCCGGY	1 cut(s)	1491
BssECI	CCNNGG	7 cut(s)	282, 462, 537, 587, 2008, 2237, 2502
BssMI	GATC	7 cut(s)	205, 213, 659, 1279, 1816, 1933, 2233
BssNI	GRCGYC	2 cut(s)	5, 1631
BssSI	CACGAG	1 cut(s)	2062
BssT1I	CCWWGG	4 cut(s)	282, 462, 587, 2502
Bst2BI	CACGAG	1 cut(s)	2062
Bst2UI	CCWGG	5 cut(s)	548, 720, 873, 1571, 2010
Bst4CI	ACNGT	7 cut(s)	813, 1135, 1156, 1378, 1403, 1755, 2376
Bst6I	CTCTTC	1 cut(s)	84
BstACI	GRCGYC	2 cut(s)	5, 1631
BstBAI	YACGTR	1 cut(s)	1265
BstBI	TTCGAA	1 cut(s)	1591
BstC8I	GCNNGC	5 cut(s)	174, 441, 483, 693, 2044
BstDEI	CTNAG	8 cut(s)	561, 777, 1515, 1520, 2193, 2388, 2447, 2493
BstDSI	CCRYGG	2 cut(s)	282, 462
BstEII	GGTNACC	1 cut(s)	1561
BstF5I	GGATG	8 cut(s)	547, 1042, 1090, 1111, 1558, 2119, 2155, 2389
BstFNI	CGCG	2 cut(s)	204, 2532
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	5 cut(s)	7, 829, 847, 1775, 2202
BstKTI	GATC	7 cut(s)	208, 216, 662, 1282, 1819, 1936, 2236
BstMAI	GTCTC	4 cut(s)	224, 752, 1444, 1744
BstMBI	GATC	7 cut(s)	205, 213, 659, 1279, 1816, 1933, 2233
BstMCI	CGRYCG	2 cut(s)	208, 2482
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 182, 436, 523, 1339, 1643, 1663, 2472, 2498
BstNI	CCWGG	5 cut(s)	548, 720, 873, 1571, 2010
BstNSI	RCATGY	1 cut(s)	1682
BstPI	GGTNACC	1 cut(s)	1561
BstSCI	CCNGG	5 cut(s)	546, 718, 871, 1569, 2008
BstSFI	CTRYAG	4 cut(s)	928, 1335, 2040, 2454
BstUI	CGCG	2 cut(s)	204, 2532
BstV1I	GCAGC	6 cut(s)	538, 811, 841, 2078, 2184, 2487
BstV2I	GAAGAC	2 cut(s)	1580, 1832
BstX2I	RGATCY	1 cut(s)	659
BstYI	RGATCY	1 cut(s)	659
BsuI	GTATCC	2 cut(s)	716, 1745
BsuRI	GGCC	7 cut(s)	546, 636, 1450, 1646, 1994, 2220, 2361
BtgI	CCRYGG	2 cut(s)	282, 462
BtsCI	GGATG	8 cut(s)	547, 1042, 1090, 1111, 1558, 2119, 2155, 2389
BtsI	GCAGTG	2 cut(s)	937, 2451
BtsIMutI	CAGTG	6 cut(s)	937, 1049, 1408, 2108, 2325, 2451
BveI	ACCTGC	2 cut(s)	246, 1676
Cac8I	GCNNGC	5 cut(s)	174, 441, 483, 693, 2044
CaiI	CAGNNNCTG	2 cut(s)	1759, 2462
CciI	TCATGA	1 cut(s)	1845
CfoI	GCGC	5 cut(s)	7, 829, 847, 1775, 2202
Cfr10I	RCCGGY	1 cut(s)	1491
Cfr13I	GGNCC	1 cut(s)	1573
CseI	GACGC	3 cut(s)	187, 1297, 1620
DdeI	CTNAG	8 cut(s)	561, 777, 1515, 1520, 2193, 2388, 2447, 2493
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	7 cut(s)	207, 215, 661, 1281, 1818, 1935, 2235
DpnII	GATC	7 cut(s)	205, 213, 659, 1279, 1816, 1933, 2233
DraI	TTTAAA	1 cut(s)	1413
DraIII	CACNNNGTG	1 cut(s)	1277
EaeI	YGGCCR	6 cut(s)	544, 634, 1448, 1644, 1992, 2359
Eam1104I	CTCTTC	1 cut(s)	84
EarI	CTCTTC	1 cut(s)	84
EciI	GGCGGA	1 cut(s)	34
Ecl136II	GAGCTC	1 cut(s)	1519
Eco130I	CCWWGG	4 cut(s)	282, 462, 587, 2502
Eco24I	GRGCYC	2 cut(s)	1521, 1811
Eco32I	GATATC	1 cut(s)	1942
Eco47I	GGWCC	1 cut(s)	1573
Eco53kI	GAGCTC	1 cut(s)	1519
Eco57I	CTGAAG	5 cut(s)	926, 929, 1185, 1833, 2453
Eco88I	CYCGRG	1 cut(s)	840
Eco91I	GGTNACC	1 cut(s)	1561
EcoICRI	GAGCTC	1 cut(s)	1519
EcoO65I	GGTNACC	1 cut(s)	1561
EcoRII	CCWGG	5 cut(s)	546, 718, 871, 1569, 2008
EcoRV	GATATC	1 cut(s)	1942
EcoT14I	CCWWGG	4 cut(s)	282, 462, 587, 2502
EcoT38I	GRGCYC	2 cut(s)	1521, 1811
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	4 cut(s)	282, 462, 587, 2502
Esp3I	CGTCTC	1 cut(s)	224
FalI	AAGNNNNNCTT	2 cut(s)	427, 459
FaqI	GGGAC	2 cut(s)	994, 2174
FauNDI	CATATG	1 cut(s)	1218
FblI	GTMKAC	1 cut(s)	1431
Fnu4HI	GCNGC	8 cut(s)	411, 527, 825, 830, 2092, 2198, 2476, 2530
FokI	GGATG	8 cut(s)	554, 1029, 1097, 1118, 1565, 2126, 2162, 2396
FriOI	GRGCYC	2 cut(s)	1521, 1811
Fsp4HI	GCNGC	8 cut(s)	411, 527, 825, 830, 2092, 2198, 2476, 2530
FspBI	CTAG	2 cut(s)	455, 572
FspI	TGCGCA	2 cut(s)	828, 2201
GlaI	GCGC	5 cut(s)	6, 828, 846, 1774, 2201
GluI	GCNGC	8 cut(s)	411, 527, 825, 830, 2092, 2198, 2476, 2530
GsaI	CCCAGC	2 cut(s)	434, 1302
GsuI	CTGGAG	3 cut(s)	401, 894, 2336
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	7 cut(s)	546, 636, 1450, 1646, 1994, 2220, 2361
HapII	CCGG	5 cut(s)	65, 167, 1061, 1492, 2482
HgaI	GACGC	3 cut(s)	187, 1297, 1620
HhaI	GCGC	5 cut(s)	7, 829, 847, 1775, 2202
Hin1I	GRCGYC	2 cut(s)	5, 1631
Hin6I	GCGC	5 cut(s)	5, 827, 845, 1773, 2200
HinP1I	GCGC	5 cut(s)	5, 827, 845, 1773, 2200
HincII	GTYRAC	1 cut(s)	1927
HindII	GTYRAC	1 cut(s)	1927
HindIII	AAGCTT	2 cut(s)	1202, 2406
HinfI	GANTC	9 cut(s)	41, 61, 256, 458, 818, 960, 1248, 1607, 2309
HpaII	CCGG	5 cut(s)	65, 167, 1061, 1492, 2482
HphI	GGTGA	3 cut(s)	1525, 1573, 2354
Hpy166II	GTNNAC	2 cut(s)	1432, 1927
Hpy188I	TCNGA	5 cut(s)	339, 626, 1003, 1821, 2085
Hpy8I	GTNNAC	2 cut(s)	1432, 1927
Hpy99I	CGWCG	1 cut(s)	100
HpyAV	CCTTC	4 cut(s)	429, 1085, 1169, 1951
HpyCH4III	ACNGT	7 cut(s)	813, 1135, 1156, 1378, 1403, 1755, 2376
HpyCH4IV	ACGT	2 cut(s)	233, 1264
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 182, 436, 523, 1339, 1643, 1663, 2472, 2498
HpyF3I	CTNAG	8 cut(s)	561, 777, 1515, 1520, 2193, 2388, 2447, 2493
HpySE526I	ACGT	2 cut(s)	233, 1264
Hsp92I	GRCGYC	2 cut(s)	5, 1631
HspAI	GCGC	5 cut(s)	5, 827, 845, 1773, 2200
KasI	GGCGCC	1 cut(s)	4
Kpn2I	TCCGGA	1 cut(s)	1060
Kzo9I	GATC	7 cut(s)	205, 213, 659, 1279, 1816, 1933, 2233
LguI	GCTCTTC	1 cut(s)	84
LmnI	GCTCC	4 cut(s)	169, 1063, 2273, 2283
Lsp1109I	GCAGC	6 cut(s)	538, 811, 841, 2078, 2184, 2487
LweI	GCATC	8 cut(s)	343, 382, 397, 779, 868, 944, 1059, 2374
MaeI	CTAG	2 cut(s)	455, 572
MaeII	ACGT	2 cut(s)	233, 1264
MaeIII	GTNAC	5 cut(s)	1120, 1182, 1561, 1949, 2022
MalI	GATC	7 cut(s)	207, 215, 661, 1281, 1818, 1935, 2235
MboI	GATC	7 cut(s)	205, 213, 659, 1279, 1816, 1933, 2233
MfeI	CAATTG	2 cut(s)	1394, 2487
MflI	RGATCY	1 cut(s)	659
MhlI	GDGCHC	2 cut(s)	1521, 1811
MlsI	TGGCCA	4 cut(s)	546, 1450, 1646, 2361
MluNI	TGGCCA	4 cut(s)	546, 1450, 1646, 2361
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	35, 452, 2303
MmeI	TCCRAC	2 cut(s)	135, 241
Mox20I	TGGCCA	4 cut(s)	546, 1450, 1646, 2361
MroI	TCCGGA	1 cut(s)	1060
MscI	TGGCCA	4 cut(s)	546, 1450, 1646, 2361
MseI	TTAA	7 cut(s)	786, 801, 902, 1380, 1412, 1544, 1796
MslI	CAYNNNNRTG	3 cut(s)	1256, 1724, 1844
Msp20I	TGGCCA	4 cut(s)	546, 1450, 1646, 2361
MspA1I	CMGCKG	3 cut(s)	379, 529, 2180
MspI	CCGG	5 cut(s)	65, 167, 1061, 1492, 2482
MspR9I	CCNGG	5 cut(s)	548, 720, 873, 1571, 2010
MunI	CAATTG	2 cut(s)	1394, 2487
MvaI	CCWGG	5 cut(s)	548, 720, 873, 1571, 2010
MvnI	CGCG	2 cut(s)	204, 2532
MwoI	GCNNNNNNNGC	9 cut(s)	11, 182, 436, 523, 1339, 1643, 1663, 2472, 2498
NarI	GGCGCC	1 cut(s)	5
NcoI	CCATGG	2 cut(s)	282, 462
NdeI	CATATG	1 cut(s)	1218
NdeII	GATC	7 cut(s)	205, 213, 659, 1279, 1816, 1933, 2233
NlaIV	GGNNCC	2 cut(s)	6, 661
NmeAIII	GCCGAG	2 cut(s)	562, 2020
NmuCI	GTSAC	4 cut(s)	1120, 1182, 1561, 2022
NsbI	TGCGCA	2 cut(s)	828, 2201
NspI	RCATGY	1 cut(s)	1682
NspV	TTCGAA	1 cut(s)	1591
PaeR7I	CTCGAG	1 cut(s)	840
PagI	TCATGA	1 cut(s)	1845
PaqCI	CACCTGC	1 cut(s)	1676
PciSI	GCTCTTC	1 cut(s)	84
PcsI	WCGNNNNNNNCGW	2 cut(s)	189, 1270
PfeI	GAWTC	6 cut(s)	61, 256, 818, 960, 1248, 1607
PflFI	GACNNNGTC	2 cut(s)	197, 1436
PfoI	TCCNGGA	2 cut(s)	718, 871
PkrI	GCNGC	8 cut(s)	412, 528, 826, 831, 2093, 2199, 2477, 2531
Ple19I	CGATCG	1 cut(s)	208
PleI	GAGTC	3 cut(s)	35, 452, 2303
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	3 cut(s)	35, 452, 2303
Ppu21I	YACGTR	1 cut(s)	1265
Psp124BI	GAGCTC	1 cut(s)	1521
Psp6I	CCWGG	5 cut(s)	546, 718, 871, 1569, 2008
PspEI	GGTNACC	1 cut(s)	1561
PspFI	CCCAGC	2 cut(s)	430, 1298
PspGI	CCWGG	5 cut(s)	546, 718, 871, 1569, 2008
PspN4I	GGNNCC	2 cut(s)	6, 661
PspPI	GGNCC	1 cut(s)	1573
PsrI	GAACNNNNNNTAC	4 cut(s)	218, 250, 503, 535
PstI	CTGCAG	3 cut(s)	932, 2044, 2458
PstNI	CAGNNNCTG	2 cut(s)	1759, 2462
PsuI	RGATCY	1 cut(s)	659
PsyI	GACNNNGTC	2 cut(s)	197, 1436
PvuI	CGATCG	1 cut(s)	208
PvuII	CAGCTG	2 cut(s)	379, 2180
RgaI	GCGATCGC	1 cut(s)	208
RseI	CAYNNNNRTG	3 cut(s)	1256, 1724, 1844
SacI	GAGCTC	1 cut(s)	1521
SapI	GCTCTTC	1 cut(s)	84
SaqAI	TTAA	7 cut(s)	786, 801, 902, 1380, 1412, 1544, 1796
SatI	GCNGC	8 cut(s)	411, 527, 825, 830, 2092, 2198, 2476, 2530
Sau3AI	GATC	7 cut(s)	205, 213, 659, 1279, 1816, 1933, 2233
Sau96I	GGNCC	1 cut(s)	1573
ScaI	AGTACT	1 cut(s)	2038
SchI	GAGTC	3 cut(s)	35, 452, 2303
ScrFI	CCNGG	5 cut(s)	548, 720, 873, 1571, 2010
SduI	GDGCHC	2 cut(s)	1521, 1811
SfaAI	GCGATCGC	1 cut(s)	208
SfaNI	GCATC	8 cut(s)	343, 382, 397, 779, 868, 944, 1059, 2374
SfcI	CTRYAG	4 cut(s)	928, 1335, 2040, 2454
SfoI	GGCGCC	1 cut(s)	6
Sfr274I	CTCGAG	1 cut(s)	840
SfuI	TTCGAA	1 cut(s)	1591
SgfI	GCGATCGC	1 cut(s)	208
SgrAI	CRCCGGYG	1 cut(s)	1491
SinI	GGWCC	1 cut(s)	1573
SlaI	CTCGAG	1 cut(s)	840
SmiMI	CAYNNNNRTG	3 cut(s)	1256, 1724, 1844
SmlI	CTYRAG	3 cut(s)	840, 1172, 1283
SmoI	CTYRAG	3 cut(s)	840, 1172, 1283
SsiI	CCGC	7 cut(s)	45, 72, 189, 202, 410, 529, 2530
SspDI	GGCGCC	1 cut(s)	4
SspI	AATATT	1 cut(s)	1765
SspMI	CTAG	2 cut(s)	455, 572
SstI	GAGCTC	1 cut(s)	1521
StyD4I	CCNGG	5 cut(s)	546, 718, 871, 1569, 2008
StyI	CCWWGG	4 cut(s)	282, 462, 587, 2502
TaaI	ACNGT	7 cut(s)	813, 1135, 1156, 1378, 1403, 1755, 2376
TaiI	ACGT	2 cut(s)	236, 1267
TaqI	TCGA	4 cut(s)	20, 162, 841, 1591
TatI	WGTACW	2 cut(s)	1668, 2036
TauI	GCSGC	2 cut(s)	413, 2532
TfiI	GAWTC	6 cut(s)	61, 256, 818, 960, 1248, 1607
Tru1I	TTAA	7 cut(s)	786, 801, 902, 1380, 1412, 1544, 1796
Tru9I	TTAA	7 cut(s)	786, 801, 902, 1380, 1412, 1544, 1796
TscAI	CASTG	6 cut(s)	937, 1056, 1408, 2115, 2332, 2458
TseFI	GTSAC	4 cut(s)	1120, 1182, 1561, 2022
TseI	GCWGC	6 cut(s)	526, 824, 829, 2091, 2197, 2475
Tsp45I	GTSAC	4 cut(s)	1120, 1182, 1561, 2022
TspGWI	ACGGA	3 cut(s)	740, 1565, 2123
TspRI	CASTG	6 cut(s)	937, 1056, 1408, 2115, 2332, 2458
Tth111I	GACNNNGTC	2 cut(s)	197, 1436
VpaK11BI	GGWCC	1 cut(s)	1573
XapI	RAATTY	7 cut(s)	131, 325, 475, 578, 1293, 1351, 2075
XbaI	TCTAGA	1 cut(s)	454
XceI	RCATGY	1 cut(s)	1682
XhoI	CTCGAG	1 cut(s)	840
XmiI	GTMKAC	1 cut(s)	1431
XspI	CTAG	2 cut(s)	455, 572
ZrmI	AGTACT	1 cut(s)	2038

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.