Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008410

Physical Location & Seq

Genomic Coordinates Forward (+)

29762707 .. 29766950

Gene Structure 4244 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1167500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGGCACCTACTGCGAAATCGGACGGAAAAACAATCATAGACTCCGCCATTTCATCCAAGATTCCGGCGACCGCAAACCCTTTAGCCGAAGAACCGTCGCAGATTGCTTCCAACATCAATTACCACGCGAAATTCAGCCCCCACTTCTCGCCGTTCAAGTTCGAGCCGGAGCAAGCCTACTACGCGACAGCTGACAGCGTCCGCGATCGCCTGATCCAGCAATGGAACGAGACGTACCTGCATTTCCACCAGGAGAATCCCAAGCAGACATACTACTTGTCCATGGAGTATCTTCAAGGACGAGCCTTGACCAATGCGATCGGGAATTTGAATGTTCAGAGTGCTTATGCTGATGCTTTGAACAAGTTGGGGCATCAGCTGGAGGAAATAACAGAGCAGCACTCGGAAATGGGGGCTGGGAAGGCTTGCTTCGTGCTTTCTAGACTCCATGGCAACGCTAAATTTACCTGCGTGGGGGTATGGTTTTTGAGGTACAGATATGGTCTATTCAAGCAGCGGATCGCCAAGGATGGCCAGGAAGAAACTGCAGAGGATTGGCTAGAGAAGTTTAGTCCGTGGGAAGTTGTCAGGCATGATGTTGTGTACCCTGTCAGATTCTTTGGCCATGTTCATGTCAATCCTGATGGATCCCGAAAGTGGGTTGAGGGAGAGGTTTTGCGAGCTCTGGCTTATGATGTGCCAATTCCAGGATACAAAACCAAGAACACTATTAGTCTTCGTCTATGGGAAGCTAAAGCATCCGCTGAGGACTTTAACCTATTTCAATTTAATGATGGACAGTATGAATCTGCTGCGCAGCTTCATTCTCGAGCGCAACAGATTTGTGCTGTTTTGTATCCTGGAGATGCCACAGAGGAGGGGAAACTTTTAAGGCTGAAGCAACAATTCTTCCTGTGCAGTGCATCACTTCAGGATATTATTTTTAGATTCAAGGAGAGGAAACAGGGGACAGACTCATTGCAATGGTCAGAATTTCCAAAAAAGGTTGCTGTCCAAATGAACGATACTCATCCTACACTTGCAATCCCAGAGCTAATGCGATTGCTATTGGATGAGGAAGGGCTTGCGTGGGATGAGGCATGGGAAGTGACAACAAGGACGGTTGCTTATACTAATCACACAGTCCTTCCTGAAGCACTTGAGAAATGGTCACAAACTGTAATGTGGAAGCTTCTCCCTCGCCATATGGAAATAATAGAAGAAATTGACAAAAGATTCATTGCTATGGTACGTGCTACACGAACTGATCTTGAGAGCAAAATTCCCAGCTTGTGCGTTTTGGATAACAATCCCCAAAAGCCTGTTGTGCGTATGGCAAAATTTATGTGTGGTGTCTGCACATACGGTAAGACATCTATTTTGGGAAATTTACAAGTTAATGGTGTTGCCCAATTGCACAGTGATATTTTGAAGAATGAGTTATTTGTAGACTATGTCTCTATATGGCCAACAAAGTTCCAAAACAAAACCAATGGGATTACTCCTCGTCGGTGGCTTCGCTTTTGCAGTCCTGAGCTCAGTAACATAATAACCAAATGGTTAAAAACTGATGAATGGGTTACCAACCTGGACCTACTTGTAGGTCTTCGAAAGTTTGCCGATGATTCAAAATTACAAGAAGAATGGGCGTCTGCCAAGATGGCTAATAAGAAGCGTTTGGCACAGTACATAGAGCATGTGGCAGGTGTCAAGATTGACCCAAACAGTCTATTTGACATACAAGTGAAGCGTATCCATGAATATAAGAGACAGTTGCTGAATATTCTGGGTGCAGTTTATCGGTATAAGAAGTTNNNN

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004645 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005977 GO:0005980 GO:0006073 GO:0006091 GO:0006112 GO:0006950 GO:0008144 GO:0008150 GO:0008152 GO:0008184 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010035 GO:0010038 GO:0015980 GO:0016052 GO:0016740 GO:0016757 GO:0016758 GO:0019842 GO:0030170 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048037 GO:0050662 GO:0050896 GO:0055114 GO:0070279 GO:0071704 GO:0097159 GO:1901363 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 ko04217 ko04910 ko04922 ko04931 map00500 map01100 map01110 map02026 map04217 map04910 map04922 map04931

Pfam Domains

Protein Families

Phosphorylase

Protein Analysis

606

Amino Acids

69.54

Weight (kDa)

8.21

Isoelectric Point (pI)

42.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphorylase	PF00343	121 - 604	1.9e-168	Carbohydrate phosphorylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46970
fragaria_vesca	FvH4_3g37910 FvH4_3g37910 FvH4_3g37910 FvH4_3g37910 FvH4_3g37910
malus_domestica	MD03G1077800.v1.1 MD05G1167500.v1.1 MD11G1082200.v1.1
prunus_persica	Prupe.6G062600_v2.0.a1
pyrus_communis	pycom03g06170 pycom11g07050
rosa_chinensis	RchiOBHm_Chr5g0068321
rosa_laevigata	RLG00000035965
rosa_multiflora	Rmu_sc0001418.1_g000022
rosa_roxburghii	Rroxscaffold_1G00012440 Rroxscaffold_1G00013180
rosa_rugosa	Rorug05G0388800
rosa_samantha	Rh5AG447300 Rh5BG465400 Rh5CG487000 Rh5DG479000
rosa_wichuraiana	Rw5G041770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1694
Acc16I	TGCGCA	1 cut(s)	816
Acc36I	ACCTGC	3 cut(s)	246, 476, 1694
AccB1I	GGYRCC	1 cut(s)	4
AccI	GTMKAC	1 cut(s)	1449
AccII	CGCG	3 cut(s)	128, 185, 204
AciI	CCGC	5 cut(s)	45, 72, 202, 517, 762
AclWI	GGATC	4 cut(s)	208, 527, 642, 655
AcoI	YGGCCR	3 cut(s)	532, 622, 1466
AcsI	RAATTY	7 cut(s)	131, 325, 461, 992, 1281, 1340, 1387
AcuI	CTGAAG	3 cut(s)	914, 917, 1173
AcyI	GRCGYC	1 cut(s)	1649
AfaI	GTAC	5 cut(s)	236, 494, 605, 1251, 1688
AfiI	CCNNNNNNNGG	4 cut(s)	474, 657, 658, 707
AgsI	TTSAA	9 cut(s)	157, 296, 331, 361, 511, 785, 952, 1432, 1629
AjnI	CCWGG	5 cut(s)	249, 534, 706, 859, 1587
AluBI	AGCT	9 cut(s)	191, 379, 683, 752, 820, 1054, 1192, 1290, 1537
AluI	AGCT	9 cut(s)	191, 379, 683, 752, 820, 1054, 1192, 1290, 1537
Alw21I	GWGCWC	2 cut(s)	685, 1539
Alw26I	GTCTC	3 cut(s)	224, 1462, 1762
AlwI	GGATC	4 cut(s)	208, 527, 642, 655
AlwNI	CAGNNNCTG	1 cut(s)	1777
Ama87I	CYCGRG	1 cut(s)	828
AoxI	GGCC	3 cut(s)	532, 622, 1466
ApeKI	GCWGC	4 cut(s)	397, 514, 812, 817
ApoI	RAATTY	7 cut(s)	131, 325, 461, 992, 1281, 1340, 1387
ArsI	GACNNNNNNTTYG	2 cut(s)	1363, 1395
AsiSI	GCGATCGC	1 cut(s)	208
AspLEI	GCGC	2 cut(s)	817, 835
AspS9I	GGNCC	1 cut(s)	1591
AsuII	TTCGAA	1 cut(s)	1609
AvaI	CYCGRG	1 cut(s)	828
AvaII	GGWCC	1 cut(s)	1591
BalI	TGGCCA	3 cut(s)	534, 624, 1468
BamHI	GGATCC	1 cut(s)	647
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	2 cut(s)	685, 1539
BbsI	GAAGAC	2 cut(s)	728, 1598
Bbv12I	GWGCWC	2 cut(s)	685, 1539
BbvCI	CCTCAGC	1 cut(s)	765
BbvI	GCAGC	4 cut(s)	409, 526, 799, 829
BccI	CCATC	4 cut(s)	524, 638, 788, 1654
BceAI	ACGGC	1 cut(s)	136
BciT130I	CCWGG	5 cut(s)	251, 536, 708, 861, 1589
BciVI	GTATCC	3 cut(s)	704, 867, 1763
BcoDI	GTCTC	3 cut(s)	224, 1462, 1762
BfaI	CTAG	2 cut(s)	441, 560
BfmI	CTRYAG	1 cut(s)	546
BfuAI	ACCTGC	3 cut(s)	246, 476, 1694
BfuI	GTATCC	3 cut(s)	704, 867, 1763
BglI	GCCNNNNNGGC	1 cut(s)	1661
BisI	GCNGC	4 cut(s)	398, 515, 813, 818
BlsI	GCNGC	4 cut(s)	399, 516, 814, 819
Bme1390I	CCNGG	5 cut(s)	251, 536, 708, 861, 1589
Bme18I	GGWCC	1 cut(s)	1591
BmeT110I	CYCGRG	1 cut(s)	828
BmgT120I	GGNCC	1 cut(s)	1591
BmiI	GGNNCC	2 cut(s)	6, 649
BmrFI	CCNGG	5 cut(s)	251, 536, 708, 861, 1589
BmsI	GCATC	5 cut(s)	343, 382, 767, 856, 932
BpiI	GAAGAC	2 cut(s)	728, 1598
BplI	GAGNNNNNCTC	2 cut(s)	386, 418
BpmI	CTGGAG	2 cut(s)	401, 882
Bpu10I	CCTNAGC	2 cut(s)	765, 1533
Bpu14I	TTCGAA	1 cut(s)	1609
BpuEI	CTTGAG	2 cut(s)	1181, 1292
BsaAI	YACGTR	1 cut(s)	1253
BsaBI	GATNNNNATC	2 cut(s)	1029, 1308
BsaHI	GRCGYC	1 cut(s)	1649
BsaJI	CCNNGG	4 cut(s)	282, 448, 525, 575
Bsc4I	CCNNNNNNNGG	4 cut(s)	474, 657, 658, 707
Bse3DI	GCAATG	4 cut(s)	227, 977, 989, 1239
Bse8I	GATNNNNATC	2 cut(s)	1029, 1308
BseBI	CCWGG	5 cut(s)	251, 536, 708, 861, 1589
BseDI	CCNNGG	4 cut(s)	282, 448, 525, 575
BseGI	GGATG	6 cut(s)	53, 535, 758, 1030, 1078, 1099
BseJI	GATNNNNATC	2 cut(s)	1029, 1308
BseLI	CCNNNNNNNGG	4 cut(s)	474, 657, 658, 707
BseMI	GCAATG	4 cut(s)	227, 977, 989, 1239
BseMII	CTCAG	3 cut(s)	756, 1524, 1552
BseRI	GAGGAG	2 cut(s)	890, 1494
BseXI	GCAGC	4 cut(s)	409, 526, 799, 829
BseYI	CCCAGC	2 cut(s)	416, 1286
BsgI	GTGCAG	3 cut(s)	937, 1342, 1812
Bsh1236I	CGCG	3 cut(s)	128, 185, 204
Bsh1285I	CGRYCG	3 cut(s)	72, 208, 321
BshFI	GGCC	3 cut(s)	534, 624, 1468
BshNI	GGYRCC	1 cut(s)	4
BsiEI	CGRYCG	3 cut(s)	72, 208, 321
BsiHKAI	GWGCWC	2 cut(s)	685, 1539
BsiHKCI	CYCGRG	1 cut(s)	828
BsiSI	CCGG	2 cut(s)	65, 167
BslFI	GGGAC	1 cut(s)	982
BslI	CCNNNNNNNGG	4 cut(s)	474, 657, 658, 707
BsmAI	GTCTC	3 cut(s)	224, 1462, 1762
BsmBI	CGTCTC	1 cut(s)	224
BsmFI	GGGAC	1 cut(s)	982
BsnI	GGCC	3 cut(s)	534, 624, 1468
BsoBI	CYCGRG	1 cut(s)	828
Bsp119I	TTCGAA	1 cut(s)	1609
Bsp1286I	GDGCHC	2 cut(s)	685, 1539
Bsp143I	GATC	6 cut(s)	205, 213, 318, 519, 647, 1267
Bsp19I	CCATGG	2 cut(s)	282, 448
BspACI	CCGC	5 cut(s)	45, 72, 202, 517, 762
BspANI	GGCC	3 cut(s)	534, 624, 1468
BspCNI	CTCAG	3 cut(s)	757, 1525, 1551
BspFNI	CGCG	3 cut(s)	128, 185, 204
BspLI	GGNNCC	2 cut(s)	6, 649
BspMAI	CTGCAG	1 cut(s)	550
BspMI	ACCTGC	3 cut(s)	246, 476, 1694
BspPI	GGATC	4 cut(s)	208, 527, 642, 655
BspT104I	TTCGAA	1 cut(s)	1609
BspT107I	GGYRCC	1 cut(s)	4
BsrDI	GCAATG	4 cut(s)	227, 977, 989, 1239
BssECI	CCNNGG	4 cut(s)	282, 448, 525, 575
BssMI	GATC	6 cut(s)	205, 213, 318, 519, 647, 1267
BssNI	GRCGYC	1 cut(s)	1649
BssT1I	CCWWGG	3 cut(s)	282, 448, 525
Bst2UI	CCWGG	5 cut(s)	251, 536, 708, 861, 1589
BstACI	GRCGYC	1 cut(s)	1649
BstAPI	GCANNNNNTGC	1 cut(s)	11
BstBAI	YACGTR	1 cut(s)	1253
BstBI	TTCGAA	1 cut(s)	1609
BstC8I	GCNNGC	4 cut(s)	174, 427, 681, 1086
BstDEI	CTNAG	3 cut(s)	765, 1533, 1538
BstDSI	CCRYGG	3 cut(s)	282, 448, 575
BstEII	GGTNACC	1 cut(s)	1579
BstF5I	GGATG	6 cut(s)	53, 535, 758, 1030, 1078, 1099
BstFNI	CGCG	3 cut(s)	128, 185, 204
BstHHI	GCGC	2 cut(s)	817, 835
BstKTI	GATC	6 cut(s)	208, 216, 321, 522, 650, 1270
BstMAI	GTCTC	3 cut(s)	224, 1462, 1762
BstMBI	GATC	6 cut(s)	205, 213, 318, 519, 647, 1267
BstMCI	CGRYCG	3 cut(s)	72, 208, 321
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 182, 422, 1327, 1661
BstNI	CCWGG	5 cut(s)	251, 536, 708, 861, 1589
BstNSI	RCATGY	1 cut(s)	1700
BstPI	GGTNACC	1 cut(s)	1579
BstSCI	CCNGG	5 cut(s)	249, 534, 706, 859, 1587
BstSFI	CTRYAG	1 cut(s)	546
BstUI	CGCG	3 cut(s)	128, 185, 204
BstV1I	GCAGC	4 cut(s)	409, 526, 799, 829
BstV2I	GAAGAC	2 cut(s)	728, 1598
BstX2I	RGATCY	1 cut(s)	647
BstYI	RGATCY	1 cut(s)	647
BsuI	GTATCC	3 cut(s)	704, 867, 1763
BsuRI	GGCC	3 cut(s)	534, 624, 1468
BtgI	CCRYGG	3 cut(s)	282, 448, 575
BtsCI	GGATG	6 cut(s)	53, 535, 758, 1030, 1078, 1099
BtsI	GCAGTG	1 cut(s)	925
BtsIMutI	CAGTG	2 cut(s)	925, 1426
BveI	ACCTGC	3 cut(s)	246, 476, 1694
Cac8I	GCNNGC	4 cut(s)	174, 427, 681, 1086
CaiI	CAGNNNCTG	1 cut(s)	1777
CfoI	GCGC	2 cut(s)	817, 835
Cfr13I	GGNCC	1 cut(s)	1591
CseI	GACGC	2 cut(s)	187, 1638
Csp6I	GTAC	5 cut(s)	235, 493, 604, 1250, 1687
CviAII	CATG	8 cut(s)	283, 449, 593, 626, 632, 1101, 1697, 1757
CviQI	GTAC	5 cut(s)	235, 493, 604, 1250, 1687
DdeI	CTNAG	3 cut(s)	765, 1533, 1538
DpnI	GATC	6 cut(s)	207, 215, 320, 521, 649, 1269
DpnII	GATC	6 cut(s)	205, 213, 318, 519, 647, 1267
EaeI	YGGCCR	3 cut(s)	532, 622, 1466
EciI	GGCGGA	1 cut(s)	34
Ecl136II	GAGCTC	2 cut(s)	683, 1537
Eco130I	CCWWGG	3 cut(s)	282, 448, 525
Eco24I	GRGCYC	2 cut(s)	685, 1539
Eco47I	GGWCC	1 cut(s)	1591
Eco53kI	GAGCTC	2 cut(s)	683, 1537
Eco57I	CTGAAG	3 cut(s)	914, 917, 1173
Eco88I	CYCGRG	1 cut(s)	828
Eco91I	GGTNACC	1 cut(s)	1579
EcoICRI	GAGCTC	2 cut(s)	683, 1537
EcoO65I	GGTNACC	1 cut(s)	1579
EcoRII	CCWGG	5 cut(s)	249, 534, 706, 859, 1587
EcoT14I	CCWWGG	3 cut(s)	282, 448, 525
EcoT38I	GRGCYC	2 cut(s)	685, 1539
ErhI	CCWWGG	3 cut(s)	282, 448, 525
Esp3I	CGTCTC	1 cut(s)	224
FaeI	CATG	8 cut(s)	286, 452, 596, 629, 635, 1104, 1700, 1760
FalI	AAGNNNNNCTT	2 cut(s)	413, 445
FaqI	GGGAC	1 cut(s)	982
FatI	CATG	8 cut(s)	282, 448, 592, 625, 631, 1100, 1696, 1756
FauNDI	CATATG	1 cut(s)	1206
FblI	GTMKAC	1 cut(s)	1449
Fnu4HI	GCNGC	4 cut(s)	398, 515, 813, 818
FokI	GGATG	6 cut(s)	40, 542, 745, 1017, 1085, 1106
FriOI	GRGCYC	2 cut(s)	685, 1539
Fsp4HI	GCNGC	4 cut(s)	398, 515, 813, 818
FspBI	CTAG	2 cut(s)	441, 560
FspI	TGCGCA	1 cut(s)	816
GlaI	GCGC	2 cut(s)	816, 834
GluI	GCNGC	4 cut(s)	398, 515, 813, 818
GsaI	CCCAGC	2 cut(s)	420, 1290
GsuI	CTGGAG	2 cut(s)	401, 882
HaeIII	GGCC	3 cut(s)	534, 624, 1468
HapII	CCGG	2 cut(s)	65, 167
HgaI	GACGC	2 cut(s)	187, 1638
HhaI	GCGC	2 cut(s)	817, 835
Hin1I	GRCGYC	1 cut(s)	1649
Hin1II	CATG	8 cut(s)	286, 452, 596, 629, 635, 1104, 1700, 1760
Hin6I	GCGC	2 cut(s)	815, 833
HinP1I	GCGC	2 cut(s)	815, 833
HindIII	AAGCTT	1 cut(s)	1190
HpaII	CCGG	2 cut(s)	65, 167
Hpy166II	GTNNAC	2 cut(s)	604, 1450
Hpy188I	TCNGA	5 cut(s)	22, 339, 406, 614, 991
Hpy8I	GTNNAC	2 cut(s)	604, 1450
Hpy99I	CGWCG	2 cut(s)	100, 1512
HpyAV	CCTTC	3 cut(s)	415, 1073, 1157
HpyCH4IV	ACGT	2 cut(s)	233, 1252
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 182, 422, 1327, 1661
HpyF3I	CTNAG	3 cut(s)	765, 1533, 1538
HpySE526I	ACGT	2 cut(s)	233, 1252
Hsp92I	GRCGYC	1 cut(s)	1649
Hsp92II	CATG	8 cut(s)	286, 452, 596, 629, 635, 1104, 1700, 1760
HspAI	GCGC	2 cut(s)	815, 833
Kzo9I	GATC	6 cut(s)	205, 213, 318, 519, 647, 1267
LmnI	GCTCC	1 cut(s)	169
Lsp1109I	GCAGC	4 cut(s)	409, 526, 799, 829
LweI	GCATC	5 cut(s)	343, 382, 767, 856, 932
MaeI	CTAG	2 cut(s)	441, 560
MaeII	ACGT	2 cut(s)	233, 1252
MaeIII	GTNAC	4 cut(s)	1108, 1170, 1541, 1579
MalI	GATC	6 cut(s)	207, 215, 320, 521, 649, 1269
MboI	GATC	6 cut(s)	205, 213, 318, 519, 647, 1267
MboII	GAAGA	9 cut(s)	101, 284, 551, 728, 901, 1232, 1444, 1598, 1652
MfeI	CAATTG	1 cut(s)	1412
MflI	RGATCY	1 cut(s)	647
MhlI	GDGCHC	2 cut(s)	685, 1539
MlsI	TGGCCA	3 cut(s)	534, 624, 1468
MluNI	TGGCCA	3 cut(s)	534, 624, 1468
MlyI	GAGTC	3 cut(s)	35, 438, 968
MmeI	TCCRAC	1 cut(s)	135
Mox20I	TGGCCA	3 cut(s)	534, 624, 1468
MscI	TGGCCA	3 cut(s)	534, 624, 1468
MseI	TTAA	5 cut(s)	774, 789, 890, 1398, 1562
MslI	CAYNNNNRTG	4 cut(s)	630, 982, 1244, 1742
Msp20I	TGGCCA	3 cut(s)	534, 624, 1468
MspA1I	CMGCKG	4 cut(s)	191, 379, 517, 764
MspI	CCGG	2 cut(s)	65, 167
MspR9I	CCNGG	5 cut(s)	251, 536, 708, 861, 1589
MunI	CAATTG	1 cut(s)	1412
MvaI	CCWGG	5 cut(s)	251, 536, 708, 861, 1589
MvnI	CGCG	3 cut(s)	128, 185, 204
MwoI	GCNNNNNNNGC	5 cut(s)	11, 182, 422, 1327, 1661
NcoI	CCATGG	2 cut(s)	282, 448
NdeI	CATATG	1 cut(s)	1206
NdeII	GATC	6 cut(s)	205, 213, 318, 519, 647, 1267
NlaIII	CATG	8 cut(s)	286, 452, 596, 629, 635, 1104, 1700, 1760
NlaIV	GGNNCC	2 cut(s)	6, 649
NmuCI	GTSAC	2 cut(s)	1108, 1170
NsbI	TGCGCA	1 cut(s)	816
NspI	RCATGY	1 cut(s)	1700
NspV	TTCGAA	1 cut(s)	1609
PaeR7I	CTCGAG	1 cut(s)	828
PaqCI	CACCTGC	1 cut(s)	1694
PcsI	WCGNNNNNNNCGW	1 cut(s)	1258
PfeI	GAWTC	7 cut(s)	61, 256, 615, 806, 948, 1236, 1625
PflFI	GACNNNGTC	2 cut(s)	197, 1454
PfoI	TCCNGGA	2 cut(s)	706, 859
PkrI	GCNGC	4 cut(s)	399, 516, 814, 819
Ple19I	CGATCG	2 cut(s)	208, 321
PleI	GAGTC	3 cut(s)	35, 438, 968
PpsI	GAGTC	3 cut(s)	35, 438, 968
Ppu21I	YACGTR	1 cut(s)	1253
Psp124BI	GAGCTC	2 cut(s)	685, 1539
Psp6I	CCWGG	5 cut(s)	249, 534, 706, 859, 1587
PspEI	GGTNACC	1 cut(s)	1579
PspFI	CCCAGC	2 cut(s)	416, 1286
PspGI	CCWGG	5 cut(s)	249, 534, 706, 859, 1587
PspN4I	GGNNCC	2 cut(s)	6, 649
PspPI	GGNCC	1 cut(s)	1591
PsrI	GAACNNNNNNTAC	2 cut(s)	218, 250
PstI	CTGCAG	1 cut(s)	550
PstNI	CAGNNNCTG	1 cut(s)	1777
PsuI	RGATCY	1 cut(s)	647
PsyI	GACNNNGTC	2 cut(s)	197, 1454
PvuI	CGATCG	2 cut(s)	208, 321
PvuII	CAGCTG	2 cut(s)	191, 379
RgaI	GCGATCGC	1 cut(s)	208
RsaI	GTAC	5 cut(s)	236, 494, 605, 1251, 1688
RsaNI	GTAC	5 cut(s)	235, 493, 604, 1250, 1687
RseI	CAYNNNNRTG	4 cut(s)	630, 982, 1244, 1742
SacI	GAGCTC	2 cut(s)	685, 1539
SaqAI	TTAA	5 cut(s)	774, 789, 890, 1398, 1562
SatI	GCNGC	4 cut(s)	398, 515, 813, 818
Sau3AI	GATC	6 cut(s)	205, 213, 318, 519, 647, 1267
Sau96I	GGNCC	1 cut(s)	1591
SchI	GAGTC	3 cut(s)	35, 438, 968
ScrFI	CCNGG	5 cut(s)	251, 536, 708, 861, 1589
SduI	GDGCHC	2 cut(s)	685, 1539
SfaAI	GCGATCGC	1 cut(s)	208
SfaNI	GCATC	5 cut(s)	343, 382, 767, 856, 932
SfcI	CTRYAG	1 cut(s)	546
Sfr274I	CTCGAG	1 cut(s)	828
SfuI	TTCGAA	1 cut(s)	1609
SgfI	GCGATCGC	1 cut(s)	208
SinI	GGWCC	1 cut(s)	1591
SlaI	CTCGAG	1 cut(s)	828
SmiMI	CAYNNNNRTG	4 cut(s)	630, 982, 1244, 1742
SmlI	CTYRAG	3 cut(s)	828, 1160, 1271
SmoI	CTYRAG	3 cut(s)	828, 1160, 1271
SsiI	CCGC	5 cut(s)	45, 72, 202, 517, 762
SspI	AATATT	1 cut(s)	1783
SspMI	CTAG	2 cut(s)	441, 560
SstI	GAGCTC	2 cut(s)	685, 1539
StyD4I	CCNGG	5 cut(s)	249, 534, 706, 859, 1587
StyI	CCWWGG	3 cut(s)	282, 448, 525
TaiI	ACGT	2 cut(s)	236, 1255
TaqI	TCGA	3 cut(s)	162, 829, 1609
TatI	WGTACW	1 cut(s)	1686
TfiI	GAWTC	7 cut(s)	61, 256, 615, 806, 948, 1236, 1625
Tru1I	TTAA	5 cut(s)	774, 789, 890, 1398, 1562
Tru9I	TTAA	5 cut(s)	774, 789, 890, 1398, 1562
TscAI	CASTG	2 cut(s)	925, 1426
TseFI	GTSAC	2 cut(s)	1108, 1170
TseI	GCWGC	4 cut(s)	397, 514, 812, 817
Tsp45I	GTSAC	2 cut(s)	1108, 1170
TspDTI	ATGAA	8 cut(s)	42, 620, 812, 819, 1034, 1228, 1587, 1773
TspGWI	ACGGA	2 cut(s)	39, 564
TspRI	CASTG	2 cut(s)	925, 1426
Tth111I	GACNNNGTC	2 cut(s)	197, 1454
VpaK11BI	GGWCC	1 cut(s)	1591
XapI	RAATTY	7 cut(s)	131, 325, 461, 992, 1281, 1340, 1387
XbaI	TCTAGA	1 cut(s)	440
XceI	RCATGY	1 cut(s)	1700
XhoI	CTCGAG	1 cut(s)	828
XmiI	GTMKAC	1 cut(s)	1449
XspI	CTAG	2 cut(s)	441, 560

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.