Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0008410

Physical Location & Seq

Genomic Coordinates Forward (+)

6994449 .. 7000667

Gene Structure 6219 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1082200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2538 bp

ATGGCACCTACTGCGAAATCGGACGGAAAAACAATCATAGACTCCGCCATTTCATCCAAGATTCCGGCGACCGCAAACCCTTTAGCCGAAGAACCGTCGCAGATTGCTTCCAACATCAATTACCACGCGAAATTCAGCCCCCACTTCTCGCCGTTCAAGTTCGAGCCGGAGCAAGCCTACTACGCGACAGCTGACAGCGTCCGCGATCGCCTGATCCAGCAATGGAACGAGACGTACCTGCATTTCCACCAGGAGAATCCCAAGCAGACATACTACTTGTCCATGGAGTATCTTCAAGGACGAGCCTTGACCAATGCGATCGGGAATTTGAATGTTCAGAGTGCTTATGCTGATGCTTTGAACAAGTTGGGGCATCAGCTGGAGGAAATAACAGAGCAGGAAAAAGATGCAGCACTCGGAAATGGTGGGCTGGGAAGGCTTGCTTCGTGCTTTCTAGACTCCATGGCAACGCTAAATTTACCTGCGTGGGGGTATGGTTTGAGGTACAGATATGGTCTATTCAAGCAGCGGATCGCCAAGGATGGCCAGGAAGAAACTGCAGAGGATTGGCTAGAGAAGTTTAGTCCGTGGGAAGTTGTCAGGCATGATGTTGTGTACCCTGTCAGATTCTTTGGCCATGTTCATGTCAATCCTGATGGATCCCGAAAGTGGGTTGAGGGAGAGGTTTTGCGAGCTCTGGCTTATGATGTGCCAATTCCAGGATACAAAACCAAGAACACTATTAGTCTTCGTCTATGGGAAGCTAAAGCATCCGCTGAGGACTTTAACCTATTTCAATTTAATGATGGACAGTATGAATCTGCTGCGCAGCTTCATTCTCGAGCGCAACAGATTTGTGCTGTTTTGTATCCTGGAGATGCCACAGAGGAGGGGAAACTTTTAAGGCTGAAGCAACAATTCTTCCTGTGCAGTGCATCACTTCAGGATATTATTTTTAGATTCAAGGAGAGGAAACAGGGGACAGACTCATTGCAATGGTCAGAATTTCCAAAAAAGGTTGCTGTCCAAATGAACGATACTCATCCTACACTTGCAATCCCAGAGCTAATGCGATTGCTATTGGATGAGGAAGGGCTTGCGTGGGATGAGGCATGGGAAGTGACAACAAGGACGGTTGCTTATACTAATCACACAGTCCTTCCTGAAGCACTTGAGAAATGGTCACAAACTGTAATGTGGAAGCTTCTCCCTCGCCATATGGAAATAATAGAAGAAATTGACAAAAGATTCATTGCTATGGTACGTGCTACACGAACTGATCTTGAGAGCAAAATTCCCAGCTTGTGCGTTTTGGATAACAATCCCCAAAAGCCTGTTGTGCGTATGGCAAATTTATGTGTGGTGTCTGCACATACGGTTAATGGTGTTGCCCAATTGCACAGTGATATTTTGAAGAATGAGTTATTTGTAGACTATGTCTCTATATGGCCAACAAAGTTCCAAAACAAAACCAATGGGATTACTCCTCGTCGGTGGCTTCGCTTTTGCAGTCCTGAGCTCAGTAACATAATAACCAAATGGTTAAAAACTGATGAATGGGTTACCAACCTGGACCTACTTGTAGGTCTTCGAAAGTTTGCCGATGATTCAAAATTACAAGAAGAATGGGCGTCTGCCAAGATGGCTAATAAGAAGCGTTTGGCACAGTACATAGAGCATGTGGCAGGTGTCAAGATTGACCCAAACAGTCTATTTGACATACAAGTGAAGCGTATCCATGAATATAAGAGACAGTTGCTGAATATTCTGGGTGCAGTTTATCGGTATAAGAAGTTAAAGATGAGCCCTGAAGAGCGGAAGAAGACAACTCCACGTACTATCATGATTGGAGGAAAAGCATTTGCAACATATACAAATGCAAAAAGAATAGTCAAGCTGGTAGACGATGTTGGTGCTGTGGTCAACAAGGATCCCGATGTCAATGGCTACTTGAAGGTTGTATTTGTTCCAAATTACAATGTATCTGTTGCCGAGATACTTATCCCAGGAAGCGAGCTGTCACAACATATCAGCACTGCAGGCATGGAGGCAAGTGGCACAAGCAACATGAAATTTGCTCTGAACGGTTGCCTCATAATCGGAACATTGGATGGGGCTAATGTGGAAATCCGTGAGGAAATTGGGGAGGATAACTTTTTCCTCTTTGGTGCAACAGCAGATGAAGTCCCTAATCTGCGCAAGGACAGAGAGATCGGCCTGTTCAAACCAGATCCTCGGTTTGAAGAAGCCAAGCAGTTTGTTAGGAGTGGAGCATTTGGAAGCTACGATTACAATCCACTTCTTGACTCCCTGGAAGGGAACACCGGTTATGGTCGTGGTGATTATTTTCTTGTAGGCCATGACTTCGCACAGTATATGGATGCTCAGGCAAAAGTAGACGAAGCTTACAAGGATAGGAAAAGGTGGCAGAAGATGTCCATACTAAGCACTGCAGGGTCTGGCAAGTTCAGCAGCGACAGGACAATTGCTCAGTACGCCAAGGAAATCTGGAACATCGAAGAGTGCCGCGTACCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004645 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005977 GO:0005980 GO:0006073 GO:0006091 GO:0006112 GO:0006950 GO:0008144 GO:0008150 GO:0008152 GO:0008184 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010035 GO:0010038 GO:0015980 GO:0016052 GO:0016740 GO:0016757 GO:0016758 GO:0019842 GO:0030170 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048037 GO:0050662 GO:0050896 GO:0055114 GO:0070279 GO:0071704 GO:0097159 GO:1901363 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 ko04217 ko04910 ko04922 ko04931 map00500 map01100 map01110 map02026 map04217 map04910 map04922 map04931

Pfam Domains

Protein Families

Phosphorylase

Protein Analysis

846

Amino Acids

96.05

Weight (kDa)

6.36

Isoelectric Point (pI)

41.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphorylase	PF00343	120 - 839	3.4e-301	Carbohydrate phosphorylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46970
fragaria_vesca	FvH4_3g37910 FvH4_3g37910 FvH4_3g37910 FvH4_3g37910 FvH4_3g37910
malus_domestica	MD03G1077800.v1.1 MD05G1167500.v1.1 MD11G1082200.v1.1
prunus_persica	Prupe.6G062600_v2.0.a1
pyrus_communis	pycom03g06170 pycom11g07050
rosa_chinensis	RchiOBHm_Chr5g0068321
rosa_laevigata	RLG00000035965
rosa_multiflora	Rmu_sc0001418.1_g000022
rosa_roxburghii	Rroxscaffold_1G00012440 Rroxscaffold_1G00013180
rosa_rugosa	Rorug05G0388800
rosa_samantha	Rh5AG447300 Rh5BG465400 Rh5CG487000 Rh5DG479000
rosa_wichuraiana	Rw5G041770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1676
Acc16I	TGCGCA	2 cut(s)	828, 2198
Acc36I	ACCTGC	3 cut(s)	246, 490, 1676
AccB1I	GGYRCC	1 cut(s)	4
AccBSI	CCGCTC	1 cut(s)	1816
AccI	GTMKAC	3 cut(s)	1431, 1902, 2397
AccII	CGCG	4 cut(s)	128, 185, 204, 2529
AciI	CCGC	7 cut(s)	45, 72, 202, 529, 774, 1816, 2527
AclWI	GGATC	7 cut(s)	208, 539, 654, 667, 1925, 1938, 2225
AcoI	YGGCCR	3 cut(s)	544, 634, 1448
AcsI	RAATTY	7 cut(s)	131, 325, 475, 1004, 1293, 1351, 2072
AcuI	CTGAAG	4 cut(s)	926, 929, 1185, 1830
AcyI	GRCGYC	1 cut(s)	1631
AfaI	GTAC	8 cut(s)	236, 506, 617, 1263, 1670, 1837, 2495, 2532
AfiI	CCNNNNNNNGG	5 cut(s)	488, 669, 670, 719, 2316
AgeI	ACCGGT	1 cut(s)	2324
AjnI	CCWGG	7 cut(s)	249, 546, 718, 871, 1569, 2005, 2310
Alw21I	GWGCWC	2 cut(s)	697, 1521
Alw26I	GTCTC	3 cut(s)	224, 1444, 1744
AlwI	GGATC	7 cut(s)	208, 539, 654, 667, 1925, 1938, 2225
AlwNI	CAGNNNCTG	2 cut(s)	1759, 2459
Ama87I	CYCGRG	1 cut(s)	840
AoxI	GGCC	5 cut(s)	544, 634, 1448, 2215, 2356
ApeKI	GCWGC	5 cut(s)	410, 526, 824, 829, 2472
ApoI	RAATTY	7 cut(s)	131, 325, 475, 1004, 1293, 1351, 2072
AsiGI	ACCGGT	1 cut(s)	2324
AsiSI	GCGATCGC	1 cut(s)	208
AspLEI	GCGC	3 cut(s)	829, 847, 2199
AspS9I	GGNCC	1 cut(s)	1573
AsuHPI	GGTGA	1 cut(s)	2351
AsuII	TTCGAA	1 cut(s)	1591
AvaI	CYCGRG	1 cut(s)	840
AvaII	GGWCC	1 cut(s)	1573
BalI	TGGCCA	3 cut(s)	546, 636, 1450
BamHI	GGATCC	2 cut(s)	659, 1930
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	3 cut(s)	697, 1521, 1808
BbsI	GAAGAC	3 cut(s)	740, 1580, 1829
Bbv12I	GWGCWC	2 cut(s)	697, 1521
BbvCI	CCTCAGC	1 cut(s)	777
BbvI	GCAGC	5 cut(s)	422, 538, 811, 841, 2484
BccI	CCATC	5 cut(s)	536, 650, 800, 1636, 2105
BceAI	ACGGC	1 cut(s)	136
BcgI	CGANNNNNNTGC	2 cut(s)	1895, 1929
BciT130I	CCWGG	7 cut(s)	251, 548, 720, 873, 1571, 2007, 2312
BciVI	GTATCC	3 cut(s)	716, 879, 1745
BcoDI	GTCTC	3 cut(s)	224, 1444, 1744
BfaI	CTAG	2 cut(s)	455, 572
BfmI	CTRYAG	3 cut(s)	558, 2037, 2451
BfuAI	ACCTGC	3 cut(s)	246, 490, 1676
BfuI	GTATCC	3 cut(s)	716, 879, 1745
BglI	GCCNNNNNGGC	1 cut(s)	1643
BisI	GCNGC	6 cut(s)	411, 527, 825, 830, 2473, 2527
BlsI	GCNGC	6 cut(s)	412, 528, 826, 831, 2474, 2528
Bme1390I	CCNGG	7 cut(s)	251, 548, 720, 873, 1571, 2007, 2312
Bme18I	GGWCC	1 cut(s)	1573
BmeT110I	CYCGRG	1 cut(s)	840
BmgT120I	GGNCC	1 cut(s)	1573
BmiI	GGNNCC	3 cut(s)	6, 661, 1932
BmrFI	CCNGG	7 cut(s)	251, 548, 720, 873, 1571, 2007, 2312
BmsI	GCATC	7 cut(s)	343, 382, 397, 779, 868, 944, 2371
BpiI	GAAGAC	3 cut(s)	740, 1580, 1829
BpmI	CTGGAG	2 cut(s)	401, 894
Bpu10I	CCTNAGC	3 cut(s)	777, 1515, 2385
Bpu14I	TTCGAA	1 cut(s)	1591
BpuEI	CTTGAG	2 cut(s)	1193, 1304
BsaAI	YACGTR	2 cut(s)	1265, 1835
BsaBI	GATNNNNATC	4 cut(s)	1041, 1320, 2000, 2292
BsaHI	GRCGYC	1 cut(s)	1631
BsaJI	CCNNGG	8 cut(s)	282, 462, 537, 587, 2005, 2234, 2310, 2499
BsaWI	WCCGGW	1 cut(s)	2324
Bsc4I	CCNNNNNNNGG	5 cut(s)	488, 669, 670, 719, 2316
Bse118I	RCCGGY	1 cut(s)	2324
Bse3DI	GCAATG	4 cut(s)	227, 989, 1001, 1251
Bse8I	GATNNNNATC	4 cut(s)	1041, 1320, 2000, 2292
BseBI	CCWGG	7 cut(s)	251, 548, 720, 873, 1571, 2007, 2312
BseDI	CCNNGG	8 cut(s)	282, 462, 537, 587, 2005, 2234, 2310, 2499
BseGI	GGATG	8 cut(s)	53, 547, 770, 1042, 1090, 1111, 2116, 2386
BseJI	GATNNNNATC	4 cut(s)	1041, 1320, 2000, 2292
BseLI	CCNNNNNNNGG	5 cut(s)	488, 669, 670, 719, 2316
BseMI	GCAATG	4 cut(s)	227, 989, 1001, 1251
BseMII	CTCAG	5 cut(s)	768, 1506, 1534, 2399, 2504
BseRI	GAGGAG	2 cut(s)	902, 1476
BseXI	GCAGC	5 cut(s)	422, 538, 811, 841, 2484
BseYI	CCCAGC	2 cut(s)	430, 1298
BsgI	GTGCAG	3 cut(s)	949, 1353, 1794
Bsh1236I	CGCG	4 cut(s)	128, 185, 204, 2529
Bsh1285I	CGRYCG	3 cut(s)	72, 208, 321
BshFI	GGCC	5 cut(s)	546, 636, 1450, 2217, 2358
BshNI	GGYRCC	1 cut(s)	4
BshTI	ACCGGT	1 cut(s)	2324
BsiEI	CGRYCG	3 cut(s)	72, 208, 321
BsiHKAI	GWGCWC	2 cut(s)	697, 1521
BsiHKCI	CYCGRG	1 cut(s)	840
BsiSI	CCGG	3 cut(s)	65, 167, 2325
BslFI	GGGAC	2 cut(s)	994, 2171
BslI	CCNNNNNNNGG	5 cut(s)	488, 669, 670, 719, 2316
BsmAI	GTCTC	3 cut(s)	224, 1444, 1744
BsmBI	CGTCTC	1 cut(s)	224
BsmFI	GGGAC	2 cut(s)	994, 2171
BsnI	GGCC	5 cut(s)	546, 636, 1450, 2217, 2358
BsoBI	CYCGRG	1 cut(s)	840
Bsp119I	TTCGAA	1 cut(s)	1591
Bsp1286I	GDGCHC	3 cut(s)	697, 1521, 1808
Bsp143I	GATC	9 cut(s)	205, 213, 318, 531, 659, 1279, 1930, 2211, 2230
Bsp19I	CCATGG	2 cut(s)	282, 462
BspACI	CCGC	7 cut(s)	45, 72, 202, 529, 774, 1816, 2527
BspANI	GGCC	5 cut(s)	546, 636, 1450, 2217, 2358
BspCNI	CTCAG	5 cut(s)	769, 1507, 1533, 2398, 2503
BspFNI	CGCG	4 cut(s)	128, 185, 204, 2529
BspHI	TCATGA	1 cut(s)	1842
BspLI	GGNNCC	3 cut(s)	6, 661, 1932
BspMAI	CTGCAG	3 cut(s)	562, 2041, 2455
BspMI	ACCTGC	3 cut(s)	246, 490, 1676
BspPI	GGATC	7 cut(s)	208, 539, 654, 667, 1925, 1938, 2225
BspQI	GCTCTTC	1 cut(s)	1806
BspT104I	TTCGAA	1 cut(s)	1591
BspT107I	GGYRCC	1 cut(s)	4
BsrBI	CCGCTC	1 cut(s)	1816
BsrDI	GCAATG	4 cut(s)	227, 989, 1001, 1251
BsrFI	RCCGGY	1 cut(s)	2324
BssAI	RCCGGY	1 cut(s)	2324
BssECI	CCNNGG	8 cut(s)	282, 462, 537, 587, 2005, 2234, 2310, 2499
BssMI	GATC	9 cut(s)	205, 213, 318, 531, 659, 1279, 1930, 2211, 2230
BssNI	GRCGYC	1 cut(s)	1631
BssT1I	CCWWGG	4 cut(s)	282, 462, 537, 2499
Bst2UI	CCWGG	7 cut(s)	251, 548, 720, 873, 1571, 2007, 2312
Bst6I	CTCTTC	2 cut(s)	1806, 2514
BstACI	GRCGYC	1 cut(s)	1631
BstAPI	GCANNNNNTGC	1 cut(s)	11
BstBAI	YACGTR	2 cut(s)	1265, 1835
BstBI	TTCGAA	1 cut(s)	1591
BstC8I	GCNNGC	6 cut(s)	174, 441, 693, 1098, 2015, 2041
BstDEI	CTNAG	6 cut(s)	777, 1515, 1520, 2385, 2444, 2490
BstDSI	CCRYGG	3 cut(s)	282, 462, 587
BstEII	GGTNACC	1 cut(s)	1561
BstF5I	GGATG	8 cut(s)	53, 547, 770, 1042, 1090, 1111, 2116, 2386
BstFNI	CGCG	4 cut(s)	128, 185, 204, 2529
BstHHI	GCGC	3 cut(s)	829, 847, 2199
BstKTI	GATC	9 cut(s)	208, 216, 321, 534, 662, 1282, 1933, 2214, 2233
BstMAI	GTCTC	3 cut(s)	224, 1444, 1744
BstMBI	GATC	9 cut(s)	205, 213, 318, 531, 659, 1279, 1930, 2211, 2230
BstMCI	CGRYCG	3 cut(s)	72, 208, 321
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 182, 436, 1339, 1643, 2040, 2469, 2495
BstNI	CCWGG	7 cut(s)	251, 548, 720, 873, 1571, 2007, 2312
BstNSI	RCATGY	1 cut(s)	1682
BstPI	GGTNACC	1 cut(s)	1561
BstSCI	CCNGG	7 cut(s)	249, 546, 718, 871, 1569, 2005, 2310
BstSFI	CTRYAG	3 cut(s)	558, 2037, 2451
BstUI	CGCG	4 cut(s)	128, 185, 204, 2529
BstV1I	GCAGC	5 cut(s)	422, 538, 811, 841, 2484
BstV2I	GAAGAC	3 cut(s)	740, 1580, 1829
BstX2I	RGATCY	3 cut(s)	659, 1930, 2230
BstYI	RGATCY	3 cut(s)	659, 1930, 2230
BsuI	GTATCC	3 cut(s)	716, 879, 1745
BsuRI	GGCC	5 cut(s)	546, 636, 1450, 2217, 2358
BtgI	CCRYGG	3 cut(s)	282, 462, 587
BtsCI	GGATG	8 cut(s)	53, 547, 770, 1042, 1090, 1111, 2116, 2386
BtsI	GCAGTG	3 cut(s)	937, 2034, 2448
BtsIMutI	CAGTG	4 cut(s)	937, 1408, 2034, 2448
BveI	ACCTGC	3 cut(s)	246, 490, 1676
Cac8I	GCNNGC	6 cut(s)	174, 441, 693, 1098, 2015, 2041
CaiI	CAGNNNCTG	2 cut(s)	1759, 2459
CciI	TCATGA	1 cut(s)	1842
CfoI	GCGC	3 cut(s)	829, 847, 2199
Cfr10I	RCCGGY	1 cut(s)	2324
Cfr13I	GGNCC	1 cut(s)	1573
CseI	GACGC	2 cut(s)	187, 1620
Csp6I	GTAC	8 cut(s)	235, 505, 616, 1262, 1669, 1836, 2494, 2531
CspAI	ACCGGT	1 cut(s)	2324
CviQI	GTAC	8 cut(s)	235, 505, 616, 1262, 1669, 1836, 2494, 2531
DdeI	CTNAG	6 cut(s)	777, 1515, 1520, 2385, 2444, 2490
DpnI	GATC	9 cut(s)	207, 215, 320, 533, 661, 1281, 1932, 2213, 2232
DpnII	GATC	9 cut(s)	205, 213, 318, 531, 659, 1279, 1930, 2211, 2230
EaeI	YGGCCR	3 cut(s)	544, 634, 1448
Eam1104I	CTCTTC	2 cut(s)	1806, 2514
EarI	CTCTTC	2 cut(s)	1806, 2514
EciI	GGCGGA	1 cut(s)	34
Ecl136II	GAGCTC	2 cut(s)	695, 1519
Eco130I	CCWWGG	4 cut(s)	282, 462, 537, 2499
Eco24I	GRGCYC	3 cut(s)	697, 1521, 1808
Eco47I	GGWCC	1 cut(s)	1573
Eco53kI	GAGCTC	2 cut(s)	695, 1519
Eco57I	CTGAAG	4 cut(s)	926, 929, 1185, 1830
Eco88I	CYCGRG	1 cut(s)	840
Eco91I	GGTNACC	1 cut(s)	1561
EcoICRI	GAGCTC	2 cut(s)	695, 1519
EcoO65I	GGTNACC	1 cut(s)	1561
EcoRII	CCWGG	7 cut(s)	249, 546, 718, 871, 1569, 2005, 2310
EcoT14I	CCWWGG	4 cut(s)	282, 462, 537, 2499
EcoT38I	GRGCYC	3 cut(s)	697, 1521, 1808
ErhI	CCWWGG	4 cut(s)	282, 462, 537, 2499
Esp3I	CGTCTC	1 cut(s)	224
FalI	AAGNNNNNCTT	2 cut(s)	427, 459
FaqI	GGGAC	2 cut(s)	994, 2171
FauNDI	CATATG	1 cut(s)	1218
FblI	GTMKAC	3 cut(s)	1431, 1902, 2397
Fnu4HI	GCNGC	6 cut(s)	411, 527, 825, 830, 2473, 2527
FokI	GGATG	8 cut(s)	40, 554, 757, 1029, 1097, 1118, 2123, 2393
FriOI	GRGCYC	3 cut(s)	697, 1521, 1808
Fsp4HI	GCNGC	6 cut(s)	411, 527, 825, 830, 2473, 2527
FspBI	CTAG	2 cut(s)	455, 572
FspI	TGCGCA	2 cut(s)	828, 2198
GlaI	GCGC	3 cut(s)	828, 846, 2198
GluI	GCNGC	6 cut(s)	411, 527, 825, 830, 2473, 2527
GsaI	CCCAGC	2 cut(s)	434, 1302
GsuI	CTGGAG	2 cut(s)	401, 894
HaeIII	GGCC	5 cut(s)	546, 636, 1450, 2217, 2358
HapII	CCGG	3 cut(s)	65, 167, 2325
HgaI	GACGC	2 cut(s)	187, 1620
HhaI	GCGC	3 cut(s)	829, 847, 2199
Hin1I	GRCGYC	1 cut(s)	1631
Hin6I	GCGC	3 cut(s)	827, 845, 2197
HinP1I	GCGC	3 cut(s)	827, 845, 2197
HincII	GTYRAC	1 cut(s)	1924
HindII	GTYRAC	1 cut(s)	1924
HindIII	AAGCTT	2 cut(s)	1202, 2403
HpaII	CCGG	3 cut(s)	65, 167, 2325
HphI	GGTGA	1 cut(s)	2351
Hpy166II	GTNNAC	5 cut(s)	616, 1432, 1903, 1924, 2398
Hpy188I	TCNGA	7 cut(s)	22, 339, 419, 626, 1003, 2082, 2102
Hpy8I	GTNNAC	5 cut(s)	616, 1432, 1903, 1924, 2398
Hpy99I	CGWCG	2 cut(s)	100, 1494
HpyAV	CCTTC	5 cut(s)	429, 1085, 1169, 1948, 2309
HpyCH4IV	ACGT	3 cut(s)	233, 1264, 1834
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 182, 436, 1339, 1643, 2040, 2469, 2495
HpyF3I	CTNAG	6 cut(s)	777, 1515, 1520, 2385, 2444, 2490
HpySE526I	ACGT	3 cut(s)	233, 1264, 1834
Hsp92I	GRCGYC	1 cut(s)	1631
HspAI	GCGC	3 cut(s)	827, 845, 2197
Kzo9I	GATC	9 cut(s)	205, 213, 318, 531, 659, 1279, 1930, 2211, 2230
LguI	GCTCTTC	1 cut(s)	1806
LmnI	GCTCC	2 cut(s)	169, 2270
Lsp1109I	GCAGC	5 cut(s)	422, 538, 811, 841, 2484
LweI	GCATC	7 cut(s)	343, 382, 397, 779, 868, 944, 2371
MaeI	CTAG	2 cut(s)	455, 572
MaeII	ACGT	3 cut(s)	233, 1264, 1834
MaeIII	GTNAC	5 cut(s)	1120, 1182, 1523, 1561, 2019
MalI	GATC	9 cut(s)	207, 215, 320, 533, 661, 1281, 1932, 2213, 2232
MbiI	CCGCTC	1 cut(s)	1816
MboI	GATC	9 cut(s)	205, 213, 318, 531, 659, 1279, 1930, 2211, 2230
MfeI	CAATTG	2 cut(s)	1394, 2484
MflI	RGATCY	3 cut(s)	659, 1930, 2230
MhlI	GDGCHC	3 cut(s)	697, 1521, 1808
MlsI	TGGCCA	3 cut(s)	546, 636, 1450
MluNI	TGGCCA	3 cut(s)	546, 636, 1450
MlyI	GAGTC	4 cut(s)	35, 452, 980, 2300
MmeI	TCCRAC	1 cut(s)	135
Mox20I	TGGCCA	3 cut(s)	546, 636, 1450
MscI	TGGCCA	3 cut(s)	546, 636, 1450
MseI	TTAA	6 cut(s)	786, 801, 902, 1380, 1544, 1796
MslI	CAYNNNNRTG	4 cut(s)	642, 994, 1256, 1724
Msp20I	TGGCCA	3 cut(s)	546, 636, 1450
MspA1I	CMGCKG	4 cut(s)	191, 379, 529, 776
MspI	CCGG	3 cut(s)	65, 167, 2325
MspR9I	CCNGG	7 cut(s)	251, 548, 720, 873, 1571, 2007, 2312
MunI	CAATTG	2 cut(s)	1394, 2484
MvaI	CCWGG	7 cut(s)	251, 548, 720, 873, 1571, 2007, 2312
MvnI	CGCG	4 cut(s)	128, 185, 204, 2529
MwoI	GCNNNNNNNGC	8 cut(s)	11, 182, 436, 1339, 1643, 2040, 2469, 2495
NcoI	CCATGG	2 cut(s)	282, 462
NdeI	CATATG	1 cut(s)	1218
NdeII	GATC	9 cut(s)	205, 213, 318, 531, 659, 1279, 1930, 2211, 2230
NlaIV	GGNNCC	3 cut(s)	6, 661, 1932
NmeAIII	GCCGAG	1 cut(s)	2017
NmuCI	GTSAC	3 cut(s)	1120, 1182, 2019
NsbI	TGCGCA	2 cut(s)	828, 2198
NspI	RCATGY	1 cut(s)	1682
NspV	TTCGAA	1 cut(s)	1591
PaeR7I	CTCGAG	1 cut(s)	840
PagI	TCATGA	1 cut(s)	1842
PaqCI	CACCTGC	1 cut(s)	1676
PciSI	GCTCTTC	1 cut(s)	1806
PcsI	WCGNNNNNNNCGW	1 cut(s)	1270
PfeI	GAWTC	7 cut(s)	61, 256, 627, 818, 960, 1248, 1607
PflFI	GACNNNGTC	2 cut(s)	197, 1436
PfoI	TCCNGGA	2 cut(s)	718, 871
PinAI	ACCGGT	1 cut(s)	2324
PkrI	GCNGC	6 cut(s)	412, 528, 826, 831, 2474, 2528
Ple19I	CGATCG	2 cut(s)	208, 321
PleI	GAGTC	4 cut(s)	35, 452, 980, 2300
PpsI	GAGTC	4 cut(s)	35, 452, 980, 2300
Ppu21I	YACGTR	2 cut(s)	1265, 1835
Psp124BI	GAGCTC	2 cut(s)	697, 1521
Psp6I	CCWGG	7 cut(s)	249, 546, 718, 871, 1569, 2005, 2310
PspEI	GGTNACC	1 cut(s)	1561
PspFI	CCCAGC	2 cut(s)	430, 1298
PspGI	CCWGG	7 cut(s)	249, 546, 718, 871, 1569, 2005, 2310
PspN4I	GGNNCC	3 cut(s)	6, 661, 1932
PspPI	GGNCC	1 cut(s)	1573
PsrI	GAACNNNNNNTAC	2 cut(s)	218, 250
PstI	CTGCAG	3 cut(s)	562, 2041, 2455
PstNI	CAGNNNCTG	2 cut(s)	1759, 2459
PsuI	RGATCY	3 cut(s)	659, 1930, 2230
PsyI	GACNNNGTC	2 cut(s)	197, 1436
PvuI	CGATCG	2 cut(s)	208, 321
PvuII	CAGCTG	2 cut(s)	191, 379
RgaI	GCGATCGC	1 cut(s)	208
RsaI	GTAC	8 cut(s)	236, 506, 617, 1263, 1670, 1837, 2495, 2532
RsaNI	GTAC	8 cut(s)	235, 505, 616, 1262, 1669, 1836, 2494, 2531
RseI	CAYNNNNRTG	4 cut(s)	642, 994, 1256, 1724
SacI	GAGCTC	2 cut(s)	697, 1521
SapI	GCTCTTC	1 cut(s)	1806
SaqAI	TTAA	6 cut(s)	786, 801, 902, 1380, 1544, 1796
SatI	GCNGC	6 cut(s)	411, 527, 825, 830, 2473, 2527
Sau3AI	GATC	9 cut(s)	205, 213, 318, 531, 659, 1279, 1930, 2211, 2230
Sau96I	GGNCC	1 cut(s)	1573
SchI	GAGTC	4 cut(s)	35, 452, 980, 2300
ScrFI	CCNGG	7 cut(s)	251, 548, 720, 873, 1571, 2007, 2312
SduI	GDGCHC	3 cut(s)	697, 1521, 1808
SfaAI	GCGATCGC	1 cut(s)	208
SfaNI	GCATC	7 cut(s)	343, 382, 397, 779, 868, 944, 2371
SfcI	CTRYAG	3 cut(s)	558, 2037, 2451
Sfr274I	CTCGAG	1 cut(s)	840
SfuI	TTCGAA	1 cut(s)	1591
SgfI	GCGATCGC	1 cut(s)	208
SinI	GGWCC	1 cut(s)	1573
SlaI	CTCGAG	1 cut(s)	840
SmiMI	CAYNNNNRTG	4 cut(s)	642, 994, 1256, 1724
SmlI	CTYRAG	3 cut(s)	840, 1172, 1283
SmoI	CTYRAG	3 cut(s)	840, 1172, 1283
SsiI	CCGC	7 cut(s)	45, 72, 202, 529, 774, 1816, 2527
SspI	AATATT	1 cut(s)	1765
SspMI	CTAG	2 cut(s)	455, 572
SstI	GAGCTC	2 cut(s)	697, 1521
StyD4I	CCNGG	7 cut(s)	249, 546, 718, 871, 1569, 2005, 2310
StyI	CCWWGG	4 cut(s)	282, 462, 537, 2499
TaiI	ACGT	3 cut(s)	236, 1267, 1837
TaqI	TCGA	4 cut(s)	162, 841, 1591, 2517
TatI	WGTACW	1 cut(s)	1668
TauI	GCSGC	1 cut(s)	2529
TfiI	GAWTC	7 cut(s)	61, 256, 627, 818, 960, 1248, 1607
Tru1I	TTAA	6 cut(s)	786, 801, 902, 1380, 1544, 1796
Tru9I	TTAA	6 cut(s)	786, 801, 902, 1380, 1544, 1796
TscAI	CASTG	4 cut(s)	937, 1408, 2041, 2455
TseFI	GTSAC	3 cut(s)	1120, 1182, 2019
TseI	GCWGC	5 cut(s)	410, 526, 824, 829, 2472
Tsp45I	GTSAC	3 cut(s)	1120, 1182, 2019
TspGWI	ACGGA	3 cut(s)	39, 576, 2120
TspRI	CASTG	4 cut(s)	937, 1408, 2041, 2455
Tth111I	GACNNNGTC	2 cut(s)	197, 1436
VpaK11BI	GGWCC	1 cut(s)	1573
XapI	RAATTY	7 cut(s)	131, 325, 475, 1004, 1293, 1351, 2072
XbaI	TCTAGA	1 cut(s)	454
XceI	RCATGY	1 cut(s)	1682
XhoI	CTCGAG	1 cut(s)	840
XmiI	GTMKAC	3 cut(s)	1431, 1902, 2397
XspI	CTAG	2 cut(s)	455, 572

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.