MD03G1081500.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0018893
Physical Location & Seq
Forward (+)
6561931 .. 6565196
3266 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1081500.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGTTTGCTTGTTTCGGTCTAGAATATGTGTTCTTGCATTTAATTGCCAGGGAACTTTTGAATCTTGACGAGGTAAGCATATTAGAATGTCCGAACATGGAAACTATATTCAGAATCACAGAGGAAAATGAAGAAGAGGCGACAAAAGACATGATTTTGTTTCCAAAACTGAACACATTTGAACTAGTAGACCTGCCTCGTCTCACAAGTCTTTGTCCAGAGGGATTTACATTTTTATGGTCATCCACAATAGATATGTTTGTGATAGGATGCGAGAAATTGAAGACATTGGGTGCTGTAATTCCACAGAGAAACAAGTTAGAGAAGAACATTGAGAAGGATTCAATAACTCATGATTTCAACACTTCTCCAACACGTTCATCAAATTGGTGCCCTGCTGGATGTGGATGTACACCGTATTCAAGAGCGAATGCTCATCGCTGCATTGAAATATTGCCACGTCCAATTAATCTGGAGGTTACACCAACTAATCTTGAGGGCTCAAATGATAATGATAATCTCGAAAATCTTCAAGTAAGCAACTGTGCGTCATTAGAAGTGATTTTCCAAGTCAAGGGACCAAGGCATGAAGAAAATATCCACTCCGTTGAAGCATTCAATGAACTGAGGATGTTGGGTTTACATTATTTGCCAAGCTTAACAAGTATTTGGGAAACGGGTACTTCACAACCGATGTTCACAGGCGGCAGCTTCGGAAACTTGAAATCGTTGGACGTCGTTCATTGTAACCAGTTGAAATATTTGTTTTCGTCTTCCATAGCCAAACTTCTTGTCAGTATAGAGGACATAGAAGTTCAGAACTGTGAGCAAATAGAAGAAATCGTTGATGCAGAAGAAGAAACTGATGAAATAATTGCATTACCTAAATTGAACTCCCTCAAGCTTGGAAATCTGTCAAACCTCAAGTATTTTTGTGGTGAAGCTTATAGTTTGAAGTTGCCGTCTTTGGTGTCATTGAGGGTTATTAATTTGCAAAATTTCAAGATATTTGCTCCTAAGCTTATTGACACACATCCCCTACTGCAAGTATACATCATGTCGGATCCGAATCGACCATACATCATGTTGGAACGACCGGCTGAATGGATGGGGGACCTCAATGCCACCGTGAGGAATATTTACGACACAAGGTATGCTTTAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

389

Amino Acids

44.03

Weight (kDa)

4.82

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 4 - 97 8.8e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 172 - 276 1.5e-23 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 233 - 335 7.4e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 738
Acc36I ACCTGC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 390
AccI GTMKAC 2 cut(s) 189, 1050
AciI CCGC 1 cut(s) 705
AclWI GGATC 2 cut(s) 1058, 1071
AcsI RAATTY 1 cut(s) 997
AcyI GRCGYC 1 cut(s) 735
AfaI GTAC 2 cut(s) 412, 682
AfiI CCNNNNNNNGG 2 cut(s) 574, 1131
AflIII ACRYGT 1 cut(s) 374
AhlI ACTAGT 1 cut(s) 184
AjiI CACGTC 1 cut(s) 461
AjnI CCWGG 1 cut(s) 47
AluBI AGCT 5 cut(s) 657, 711, 904, 944, 1021
AluI AGCT 5 cut(s) 657, 711, 904, 944, 1021
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 2 cut(s) 1058, 1071
ApeKI GCWGC 2 cut(s) 441, 708
ApoI RAATTY 1 cut(s) 997
ArsI GACNNNNNNTTYG 2 cut(s) 777, 809
AseI ATTAAT 2 cut(s) 468, 987
Asp700I GAANNNNTTC 1 cut(s) 528
AspS9I GGNCC 2 cut(s) 578, 1114
AsuHPI GGTGA 1 cut(s) 950
AvaII GGWCC 2 cut(s) 578, 1114
BaeGI GKGCMC 1 cut(s) 395
BamHI GGATCC 1 cut(s) 1063
BanI GGYRCC 1 cut(s) 390
BanII GRGCYC 1 cut(s) 503
BbsI GAAGAC 2 cut(s) 290, 765
BbvI GCAGC 2 cut(s) 428, 720
BccI CCATC 1 cut(s) 1102
BceAI ACGGC 1 cut(s) 946
BciT130I CCWGG 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 206
BcuI ACTAGT 1 cut(s) 184
BfaI CTAG 2 cut(s) 20, 185
BfuAI ACCTGC 1 cut(s) 201
BisI GCNGC 3 cut(s) 442, 706, 709
BlsI GCNGC 3 cut(s) 443, 707, 710
Bme1390I CCNGG 1 cut(s) 49
Bme18I GGWCC 2 cut(s) 578, 1114
BmgBI CACGTC 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 578, 1114
BmiI GGNNCC 4 cut(s) 392, 579, 1065, 1115
BmrFI CCNGG 1 cut(s) 49
BmsI GCATC 2 cut(s) 260, 838
BpiI GAAGAC 2 cut(s) 290, 765
BpmI CTGGAG 1 cut(s) 494
Bpu10I CCTNAGC 1 cut(s) 1017
BpuEI CTTGAG 3 cut(s) 515, 884, 908
BsaBI GATNNNNATC 1 cut(s) 1068
BsaHI GRCGYC 1 cut(s) 735
BsaJI CCNNGG 2 cut(s) 48, 581
Bsc4I CCNNNNNNNGG 2 cut(s) 574, 1131
Bse118I RCCGGY 1 cut(s) 1096
Bse1I ACTGG 1 cut(s) 751
Bse8I GATNNNNATC 1 cut(s) 1068
BseBI CCWGG 1 cut(s) 49
BseDI CCNNGG 2 cut(s) 48, 581
BseGI GGATG 7 cut(s) 242, 275, 407, 413, 636, 1033, 1113
BseJI GATNNNNATC 1 cut(s) 1068
BseLI CCNNNNNNNGG 2 cut(s) 574, 1131
BseMII CTCAG 1 cut(s) 617
BseNI ACTGG 1 cut(s) 751
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 2 cut(s) 428, 720
Bsh1285I CGRYCG 1 cut(s) 1097
BshNI GGYRCC 1 cut(s) 390
BsiEI CGRYCG 1 cut(s) 1097
BsiSI CCGG 1 cut(s) 1097
BslFI GGGAC 2 cut(s) 591, 1127
BslI CCNNNNNNNGG 2 cut(s) 574, 1131
BsmAI GTCTC 1 cut(s) 206
BsmBI CGTCTC 1 cut(s) 206
BsmFI GGGAC 2 cut(s) 591, 1127
BsmI GAATGC 2 cut(s) 436, 614
Bsp1286I GDGCHC 2 cut(s) 395, 503
Bsp1407I TGTACA 1 cut(s) 410
Bsp143I GATC 1 cut(s) 1063
BspACI CCGC 1 cut(s) 705
BspCNI CTCAG 1 cut(s) 618
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 4 cut(s) 392, 579, 1065, 1115
BspMI ACCTGC 1 cut(s) 201
BspPI GGATC 2 cut(s) 1058, 1071
BspT107I GGYRCC 1 cut(s) 390
BsrFI RCCGGY 1 cut(s) 1096
BsrGI TGTACA 1 cut(s) 410
BsrI ACTGG 1 cut(s) 751
BssAI RCCGGY 1 cut(s) 1096
BssECI CCNNGG 2 cut(s) 48, 581
BssMI GATC 1 cut(s) 1063
BssNAI GTATAC 1 cut(s) 1051
BssNI GRCGYC 1 cut(s) 735
BssT1I CCWWGG 1 cut(s) 581
Bst1107I GTATAC 1 cut(s) 1051
Bst2UI CCWGG 1 cut(s) 49
Bst4CI ACNGT 4 cut(s) 417, 545, 824, 1129
Bst6I CTCTTC 1 cut(s) 129
BstACI GRCGYC 1 cut(s) 735
BstAUI TGTACA 1 cut(s) 410
BstDEI CTNAG 2 cut(s) 626, 1017
BstF5I GGATG 7 cut(s) 242, 275, 407, 413, 636, 1033, 1113
BstKTI GATC 1 cut(s) 1066
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 1 cut(s) 1063
BstMCI CGRYCG 1 cut(s) 1097
BstNI CCWGG 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 47
BstSLI GKGCMC 1 cut(s) 395
BstV1I GCAGC 2 cut(s) 428, 720
BstV2I GAAGAC 2 cut(s) 290, 765
BstX2I RGATCY 1 cut(s) 1063
BstYI RGATCY 1 cut(s) 1063
BstZ17I GTATAC 1 cut(s) 1051
BtgZI GCGATG 1 cut(s) 422
BtrI CACGTC 1 cut(s) 461
BtsCI GGATG 7 cut(s) 242, 275, 407, 413, 636, 1033, 1113
BveI ACCTGC 1 cut(s) 201
CciI TCATGA 1 cut(s) 352
Cfr10I RCCGGY 1 cut(s) 1096
Cfr13I GGNCC 2 cut(s) 578, 1114
CseI GACGC 1 cut(s) 537
Csp6I GTAC 2 cut(s) 411, 681
CviAII CATG 6 cut(s) 97, 151, 353, 587, 1057, 1084
CviJI RGCY 8 cut(s) 501, 657, 711, 782, 904, 944, 1021, 1100
CviKI_1 RGCY 8 cut(s) 501, 657, 711, 782, 904, 944, 1021, 1100
CviQI GTAC 2 cut(s) 411, 681
DdeI CTNAG 2 cut(s) 626, 1017
DpnI GATC 1 cut(s) 1065
DpnII GATC 1 cut(s) 1063
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 581
Eco24I GRGCYC 1 cut(s) 503
Eco47I GGWCC 2 cut(s) 578, 1114
EcoO109I RGGNCCY 1 cut(s) 1114
EcoRII CCWGG 1 cut(s) 47
EcoT14I CCWWGG 1 cut(s) 581
EcoT38I GRGCYC 1 cut(s) 503
ErhI CCWWGG 1 cut(s) 581
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 6 cut(s) 100, 154, 356, 590, 1060, 1087
FalI AAGNNNNNCTT 1 cut(s) 1141
FaqI GGGAC 2 cut(s) 591, 1127
FatI CATG 6 cut(s) 96, 150, 352, 586, 1056, 1083
FblI GTMKAC 2 cut(s) 189, 1050
Fnu4HI GCNGC 3 cut(s) 442, 706, 709
FokI GGATG 7 cut(s) 229, 282, 414, 420, 643, 1020, 1120
FriOI GRGCYC 1 cut(s) 503
Fsp4HI GCNGC 3 cut(s) 442, 706, 709
FspBI CTAG 2 cut(s) 20, 185
GluI GCNGC 3 cut(s) 442, 706, 709
GsuI CTGGAG 1 cut(s) 494
HapII CCGG 1 cut(s) 1097
HgaI GACGC 1 cut(s) 537
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 6 cut(s) 100, 154, 356, 590, 1060, 1087
HindIII AAGCTT 4 cut(s) 655, 902, 942, 1019
HinfI GANTC 4 cut(s) 61, 114, 341, 1069
HpaII CCGG 1 cut(s) 1097
HphI GGTGA 1 cut(s) 950
Hpy166II GTNNAC 5 cut(s) 190, 413, 641, 699, 1051
Hpy188I TCNGA 6 cut(s) 93, 113, 716, 819, 1063, 1068
Hpy188III TCNNGA 9 cut(s) 20, 65, 218, 353, 423, 473, 494, 521, 1003
Hpy8I GTNNAC 5 cut(s) 190, 413, 641, 699, 1051
Hpy99I CGWCG 1 cut(s) 740
HpyAV CCTTC 1 cut(s) 331
HpyCH4III ACNGT 4 cut(s) 417, 545, 824, 1129
HpyCH4IV ACGT 3 cut(s) 376, 460, 735
HpyCH4V TGCA 6 cut(s) 37, 444, 851, 878, 994, 1045
HpyF3I CTNAG 2 cut(s) 626, 1017
HpySE526I ACGT 3 cut(s) 376, 460, 735
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 6 cut(s) 100, 154, 356, 590, 1060, 1087
Kzo9I GATC 1 cut(s) 1063
LmnI GCTCC 1 cut(s) 1018
Lsp1109I GCAGC 2 cut(s) 428, 720
LweI GCATC 2 cut(s) 260, 838
MaeI CTAG 2 cut(s) 20, 185
MaeII ACGT 3 cut(s) 376, 460, 735
MaeIII GTNAC 2 cut(s) 478, 746
MalI GATC 1 cut(s) 1065
MboI GATC 1 cut(s) 1063
MflI RGATCY 1 cut(s) 1063
MhlI GDGCHC 2 cut(s) 395, 503
MmeI TCCRAC 4 cut(s) 395, 711, 1041, 1068
MroXI GAANNNNTTC 1 cut(s) 528
MseI TTAA 6 cut(s) 41, 468, 659, 987, 1160, 1165
MslI CAYNNNNRTG 1 cut(s) 235
MspI CCGG 1 cut(s) 1097
MspR9I CCNGG 1 cut(s) 49
Mva1269I GAATGC 2 cut(s) 436, 614
MvaI CCWGG 1 cut(s) 49
NdeII GATC 1 cut(s) 1063
NlaIII CATG 6 cut(s) 100, 154, 356, 590, 1060, 1087
NlaIV GGNNCC 4 cut(s) 392, 579, 1065, 1115
PagI TCATGA 1 cut(s) 352
PctI GAATGC 2 cut(s) 436, 614
PdmI GAANNNNTTC 1 cut(s) 528
PfeI GAWTC 4 cut(s) 61, 114, 341, 1069
PkrI GCNGC 3 cut(s) 443, 707, 710
PpuMI RGGWCCY 1 cut(s) 1114
PshBI ATTAAT 2 cut(s) 468, 987
Psp5II RGGWCCY 1 cut(s) 1114
Psp6I CCWGG 1 cut(s) 47
PspGI CCWGG 1 cut(s) 47
PspN4I GGNNCC 4 cut(s) 392, 579, 1065, 1115
PspPI GGNCC 2 cut(s) 578, 1114
PspPPI RGGWCCY 1 cut(s) 1114
PsuI RGATCY 1 cut(s) 1063
RsaI GTAC 2 cut(s) 412, 682
RsaNI GTAC 2 cut(s) 411, 681
RseI CAYNNNNRTG 1 cut(s) 235
SaqAI TTAA 6 cut(s) 41, 468, 659, 987, 1160, 1165
SatI GCNGC 3 cut(s) 442, 706, 709
Sau3AI GATC 1 cut(s) 1063
Sau96I GGNCC 2 cut(s) 578, 1114
ScrFI CCNGG 1 cut(s) 49
SduI GDGCHC 2 cut(s) 395, 503
SfaNI GCATC 2 cut(s) 260, 838
SinI GGWCC 2 cut(s) 578, 1114
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 3 cut(s) 494, 899, 923
SmoI CTYRAG 3 cut(s) 494, 899, 923
SpeI ACTAGT 1 cut(s) 184
SsiI CCGC 1 cut(s) 705
SspI AATATT 3 cut(s) 453, 761, 1138
SspMI CTAG 2 cut(s) 20, 185
StyD4I CCNGG 1 cut(s) 47
StyI CCWWGG 1 cut(s) 581
TaaI ACNGT 4 cut(s) 417, 545, 824, 1129
TaiI ACGT 3 cut(s) 379, 463, 738
TaqI TCGA 2 cut(s) 522, 1072
TaqII GACCGA 1 cut(s) 5
TatI WGTACW 1 cut(s) 410
TauI GCSGC 1 cut(s) 708
TfiI GAWTC 4 cut(s) 61, 114, 341, 1069
Tru1I TTAA 6 cut(s) 41, 468, 659, 987, 1160, 1165
Tru9I TTAA 6 cut(s) 41, 468, 659, 987, 1160, 1165
TseI GCWGC 2 cut(s) 441, 708
TspDTI ATGAA 6 cut(s) 144, 369, 603, 636, 731, 882
TspGWI ACGGA 1 cut(s) 595
VpaK11BI GGWCC 2 cut(s) 578, 1114
VspI ATTAAT 2 cut(s) 468, 987
XapI RAATTY 1 cut(s) 997
XbaI TCTAGA 1 cut(s) 19
XmiI GTMKAC 2 cut(s) 189, 1050
XmnI GAANNNNTTC 1 cut(s) 528
XspI CTAG 2 cut(s) 20, 185
ZraI GACGTC 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.