MD11G1085500.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0018893
Physical Location & Seq
Forward (+)
7200718 .. 7201466
749 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1085500.v1.1.491

Sequence Viewer

Length: 540 bp
ATGCACGTGAAAAGATGTGAAAATTTGAAGACATTGGGTGTTGTAATTCCACAAAGAAAGAAGTTGGAGAATAACTTGAAGAAGGATTCAACAAGTCATGATTTCAGCACTTCTCCAACACGTTCATCAAATTGGTGCCCTGCTGGATGTGGATGCGCACCATATTCAAGACCAAACGCCCACCGCCCCATTGAAATATTGCCACGTCCAATTAACCTGGAGGTTACACAAACTAATCTTGAGGACTCAAATGATAATGATAATCTCGAAAATCTTACTGTAAGTGACTGTAATTCGATGGAAGTGATTTTCCAACTCAAGGGACCGAAGCATGAAGAAAGTAGTCACTCCATTGAAGCATTCAATAAATTGAGTTCCTTGCGGTTAGATAGATTGCCAGGTTTAACGCGTGTTTGGGAGATGGGTAGTTCACAACCGATGTTGACAGGAAGCAGCTTCGGAAACTTGAAATCGCTGGAGGTTGGTTTTTGCAACCAGTTGAAATACTTGTTTTCATCGTCCATAGTCAAACTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

180

Amino Acids

20.05

Weight (kDa)

8.54

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 88 - 177 1e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 157
AccB1I GGYRCC 1 cut(s) 135
AccII CGCG 1 cut(s) 409
AciI CCGC 2 cut(s) 184, 382
AcsI RAATTY 1 cut(s) 22
AcvI CACGTG 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 319
AflIII ACRYGT 2 cut(s) 119, 407
AgsI TTSAA 9 cut(s) 28, 79, 90, 168, 194, 356, 364, 469, 502
AjiI CACGTC 1 cut(s) 206
AjnI CCWGG 2 cut(s) 216, 397
AluBI AGCT 1 cut(s) 456
AluI AGCT 1 cut(s) 456
ApeKI GCWGC 1 cut(s) 453
ApoI RAATTY 1 cut(s) 22
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 323
AvaII GGWCC 1 cut(s) 323
BaeGI GKGCMC 1 cut(s) 140
BanI GGYRCC 1 cut(s) 135
BbrPI CACGTG 1 cut(s) 7
BbsI GAAGAC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 465
BccI CCATC 2 cut(s) 292, 415
BciT130I CCWGG 2 cut(s) 218, 399
BisI GCNGC 1 cut(s) 454
BlsI GCNGC 1 cut(s) 455
Bme1390I CCNGG 2 cut(s) 218, 399
Bme18I GGWCC 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 2 cut(s) 137, 324
BmrFI CCNGG 2 cut(s) 218, 399
BmsI GCATC 1 cut(s) 143
BpiI GAAGAC 1 cut(s) 35
BpmI CTGGAG 2 cut(s) 239, 497
BpuEI CTTGAG 2 cut(s) 260, 302
BsaAI YACGTR 1 cut(s) 7
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 496
BseBI CCWGG 2 cut(s) 218, 399
BseGI GGATG 2 cut(s) 152, 158
BseLI CCNNNNNNNGG 1 cut(s) 319
BseNI ACTGG 1 cut(s) 496
BseSI GKGCMC 1 cut(s) 140
BseXI GCAGC 1 cut(s) 465
Bsh1236I CGCG 1 cut(s) 409
BshNI GGYRCC 1 cut(s) 135
BslFI GGGAC 1 cut(s) 336
BslI CCNNNNNNNGG 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 336
BsmI GAATGC 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 140
BspACI CCGC 2 cut(s) 184, 382
BspFNI CGCG 1 cut(s) 409
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 2 cut(s) 137, 324
BspT107I GGYRCC 1 cut(s) 135
BsrI ACTGG 1 cut(s) 496
Bst2UI CCWGG 2 cut(s) 218, 399
Bst4CI ACNGT 2 cut(s) 280, 290
BstBAI YACGTR 1 cut(s) 7
BstF5I GGATG 2 cut(s) 152, 158
BstFNI CGCG 1 cut(s) 409
BstHHI GCGC 1 cut(s) 158
BstNI CCWGG 2 cut(s) 218, 399
BstSCI CCNGG 2 cut(s) 216, 397
BstSLI GKGCMC 1 cut(s) 140
BstUI CGCG 1 cut(s) 409
BstV1I GCAGC 1 cut(s) 465
BstV2I GAAGAC 1 cut(s) 35
BtrI CACGTC 1 cut(s) 206
BtsCI GGATG 2 cut(s) 152, 158
CciI TCATGA 1 cut(s) 97
CfoI GCGC 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 323
CviAII CATG 2 cut(s) 98, 332
CviJI RGCY 1 cut(s) 456
CviKI_1 RGCY 1 cut(s) 456
Eco47I GGWCC 1 cut(s) 323
Eco72I CACGTG 1 cut(s) 7
EcoRII CCWGG 2 cut(s) 216, 397
FaeI CATG 2 cut(s) 101, 335
FaiI YATR 4 cut(s) 99, 163, 333, 524
FaqI GGGAC 1 cut(s) 336
FatI CATG 2 cut(s) 97, 331
Fnu4HI GCNGC 1 cut(s) 454
FokI GGATG 2 cut(s) 159, 165
Fsp4HI GCNGC 1 cut(s) 454
FspAI RTGCGCAY 1 cut(s) 157
FspI TGCGCA 1 cut(s) 157
GlaI GCGC 1 cut(s) 157
GluI GCNGC 1 cut(s) 454
GsuI CTGGAG 2 cut(s) 239, 497
HhaI GCGC 1 cut(s) 158
Hin1II CATG 2 cut(s) 101, 335
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HincII GTYRAC 1 cut(s) 444
HindII GTYRAC 1 cut(s) 444
HinfI GANTC 2 cut(s) 86, 245
Hpy166II GTNNAC 2 cut(s) 431, 444
Hpy188I TCNGA 1 cut(s) 461
Hpy188III TCNNGA 4 cut(s) 98, 168, 239, 266
Hpy8I GTNNAC 2 cut(s) 431, 444
HpyAV CCTTC 1 cut(s) 76
HpyCH4III ACNGT 2 cut(s) 280, 290
HpyCH4IV ACGT 3 cut(s) 6, 121, 205
HpyCH4V TGCA 2 cut(s) 4, 492
HpySE526I ACGT 3 cut(s) 6, 121, 205
Hsp92II CATG 2 cut(s) 101, 335
HspAI GCGC 1 cut(s) 156
LpnPI CCDG 9 cut(s) 129, 153, 203, 230, 384, 411, 432, 461, 509
Lsp1109I GCAGC 1 cut(s) 465
LweI GCATC 1 cut(s) 143
MaeII ACGT 3 cut(s) 6, 121, 205
MaeIII GTNAC 3 cut(s) 223, 284, 344
MboII GAAGA 3 cut(s) 40, 91, 347
MhlI GDGCHC 1 cut(s) 140
MluCI AATT 6 cut(s) 22, 45, 130, 210, 292, 368
MluI ACGCGT 1 cut(s) 407
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 3 cut(s) 45, 140, 337
MnlI CCTC 3 cut(s) 214, 235, 472
MseI TTAA 2 cut(s) 213, 404
MspR9I CCNGG 2 cut(s) 218, 399
Mva1269I GAATGC 1 cut(s) 359
MvaI CCWGG 2 cut(s) 218, 399
MvnI CGCG 1 cut(s) 409
NlaIII CATG 2 cut(s) 101, 335
NlaIV GGNNCC 2 cut(s) 137, 324
NmuCI GTSAC 2 cut(s) 284, 344
NsbI TGCGCA 1 cut(s) 157
PagI TCATGA 1 cut(s) 97
PctI GAATGC 1 cut(s) 359
PfeI GAWTC 1 cut(s) 86
PkrI GCNGC 1 cut(s) 455
PleI GAGTC 1 cut(s) 239
PmaCI CACGTG 1 cut(s) 7
PmlI CACGTG 1 cut(s) 7
PpsI GAGTC 1 cut(s) 239
Ppu21I YACGTR 1 cut(s) 7
Psp6I CCWGG 2 cut(s) 216, 397
PspCI CACGTG 1 cut(s) 7
PspGI CCWGG 2 cut(s) 216, 397
PspN4I GGNNCC 2 cut(s) 137, 324
PspPI GGNCC 1 cut(s) 323
SaqAI TTAA 2 cut(s) 213, 404
SatI GCNGC 1 cut(s) 454
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 2 cut(s) 218, 399
SduI GDGCHC 1 cut(s) 140
SetI ASST 8 cut(s) 9, 124, 208, 219, 225, 403, 458, 483
SfaNI GCATC 1 cut(s) 143
SinI GGWCC 1 cut(s) 323
SmlI CTYRAG 2 cut(s) 239, 317
SmoI CTYRAG 2 cut(s) 239, 317
Sse9I AATT 6 cut(s) 22, 45, 130, 210, 292, 368
SsiI CCGC 2 cut(s) 184, 382
SspI AATATT 1 cut(s) 198
StyD4I CCNGG 2 cut(s) 216, 397
TaaI ACNGT 2 cut(s) 280, 290
TaiI ACGT 3 cut(s) 9, 124, 208
TaqI TCGA 2 cut(s) 267, 296
TaqII GACCGA 1 cut(s) 340
TasI AATT 6 cut(s) 22, 45, 130, 210, 292, 368
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 2 cut(s) 213, 404
Tru9I TTAA 2 cut(s) 213, 404
TseFI GTSAC 2 cut(s) 284, 344
TseI GCWGC 1 cut(s) 453
Tsp45I GTSAC 2 cut(s) 284, 344
TspDTI ATGAA 3 cut(s) 114, 348, 504
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.