MD17G1142600.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0018893
Physical Location & Seq
Forward (+)
12871503 .. 12873132
1630 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1142600.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGAAGTGATTTTCCAACTCAAGGGACCGAAGCATGAAGAAAATAGTCACTCCGTTGAAGCATTCAATAAATTGAGGTTCTTGAAGTTATATACATTGCCAAGTTTAACGCGTGTTTGGGAAACGGATAGTTCACAACCGATGTTGACAGGAAGCAGCTTCGGAAACTTGAAATCGCTGAAGGTCGGTTTTTGCAACCAGTTGAAATACTTGTTTTCATCGTCCACAGTCAAACTTCTTGTGAGTATAGAGGACATAGAAGTTGAATTCTGTGAGAAGATGGAAGAAATCGTTGCCGCAGAAGAAGAAACTGATGAAATAATTACGTTACCTAAAGTGAAGATCATGTGGCTTCGAAGTCTGCCAAAACTCAAGTATTTTTGTGGTGAAGCTTATACTTTGAAGTTGCCGTCTTTGGAGGTCTTGGTGGTTACTGACGTGCAAAACTTAAGCCTATTCGCTCCAAAGCTTATTGCCACACATCCCCGATTGCAAGTACGCACCGCGTTGGGAGTGGTTGAATGGAAGGGGGACCTCAATGCCACTCTAAGGAATATTTACGTCACAAGAACAACAATTCAAAGAAGGTTGTGGCTTCAACAATTCGTTTACTATCTCATATGTACAAACCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

212

Amino Acids

24.35

Weight (kDa)

8.28

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 1 - 92 7.5e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 50 - 155 7.3e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 112, 506
AciI CCGC 2 cut(s) 297, 504
AcsI RAATTY 1 cut(s) 266
AcuI CTGAAG 1 cut(s) 200
AfaI GTAC 2 cut(s) 498, 625
AfiI CCNNNNNNNGG 2 cut(s) 22, 549
AflII CTTAAG 1 cut(s) 448
AflIII ACRYGT 1 cut(s) 110
AjiI CACGTC 1 cut(s) 439
AluBI AGCT 3 cut(s) 159, 392, 469
AluI AGCT 3 cut(s) 159, 392, 469
ApeKI GCWGC 1 cut(s) 156
ApoI RAATTY 1 cut(s) 266
AspS9I GGNCC 2 cut(s) 26, 532
AsuHPI GGTGA 1 cut(s) 398
AsuII TTCGAA 1 cut(s) 355
AvaII GGWCC 2 cut(s) 26, 532
BbvI GCAGC 1 cut(s) 168
BccI CCATC 1 cut(s) 274
BceAI ACGGC 1 cut(s) 394
BfrI CTTAAG 1 cut(s) 448
BisI GCNGC 2 cut(s) 157, 297
BlsI GCNGC 2 cut(s) 158, 298
Bme18I GGWCC 2 cut(s) 26, 532
BmgBI CACGTC 1 cut(s) 439
BmgT120I GGNCC 2 cut(s) 26, 532
BmiI GGNNCC 2 cut(s) 27, 533
Bpu14I TTCGAA 1 cut(s) 355
BpuEI CTTGAG 2 cut(s) 5, 356
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 549
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 1 cut(s) 95
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 2 cut(s) 22, 549
BseMI GCAATG 1 cut(s) 95
BseNI ACTGG 1 cut(s) 199
BseXI GCAGC 1 cut(s) 168
Bsh1236I CGCG 2 cut(s) 112, 506
BslFI GGGAC 2 cut(s) 39, 545
BslI CCNNNNNNNGG 2 cut(s) 22, 549
BsmFI GGGAC 2 cut(s) 39, 545
BsmI GAATGC 1 cut(s) 62
Bsp119I TTCGAA 1 cut(s) 355
Bsp1407I TGTACA 1 cut(s) 623
Bsp143I GATC 1 cut(s) 342
BspACI CCGC 2 cut(s) 297, 504
BspFNI CGCG 2 cut(s) 112, 506
BspLI GGNNCC 2 cut(s) 27, 533
BspT104I TTCGAA 1 cut(s) 355
BspTI CTTAAG 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 95
BsrGI TGTACA 1 cut(s) 623
BsrI ACTGG 1 cut(s) 199
BssMI GATC 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 229
BstAFI CTTAAG 1 cut(s) 448
BstAUI TGTACA 1 cut(s) 623
BstBI TTCGAA 1 cut(s) 355
BstDEI CTNAG 1 cut(s) 548
BstF5I GGATG 1 cut(s) 481
BstFNI CGCG 2 cut(s) 112, 506
BstKTI GATC 1 cut(s) 345
BstMBI GATC 1 cut(s) 342
BstUI CGCG 2 cut(s) 112, 506
BstV1I GCAGC 1 cut(s) 168
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 1 cut(s) 481
Cfr13I GGNCC 2 cut(s) 26, 532
Csp6I GTAC 2 cut(s) 497, 624
CviAII CATG 2 cut(s) 35, 346
CviJI RGCY 6 cut(s) 159, 352, 392, 453, 469, 595
CviKI_1 RGCY 6 cut(s) 159, 352, 392, 453, 469, 595
CviQI GTAC 2 cut(s) 497, 624
DdeI CTNAG 1 cut(s) 548
DpnI GATC 1 cut(s) 344
DpnII GATC 1 cut(s) 342
Eco47I GGWCC 2 cut(s) 26, 532
Eco57I CTGAAG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 532
EcoRI GAATTC 1 cut(s) 266
FaeI CATG 2 cut(s) 38, 349
FaiI YATR 9 cut(s) 36, 91, 93, 248, 257, 347, 396, 620, 622
FaqI GGGAC 2 cut(s) 39, 545
FatI CATG 2 cut(s) 34, 345
FauNDI CATATG 1 cut(s) 620
Fnu4HI GCNGC 2 cut(s) 157, 297
FokI GGATG 1 cut(s) 468
Fsp4HI GCNGC 2 cut(s) 157, 297
GluI GCNGC 2 cut(s) 157, 297
Hin1II CATG 2 cut(s) 38, 349
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HindIII AAGCTT 2 cut(s) 390, 467
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 4 cut(s) 134, 147, 225, 610
Hpy188I TCNGA 1 cut(s) 164
Hpy188III TCNNGA 1 cut(s) 82
Hpy8I GTNNAC 4 cut(s) 134, 147, 225, 610
HpyAV CCTTC 3 cut(s) 175, 520, 579
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 3 cut(s) 326, 438, 561
HpyCH4V TGCA 3 cut(s) 195, 442, 493
HpyF3I CTNAG 1 cut(s) 548
HpySE526I ACGT 3 cut(s) 326, 438, 561
Hsp92II CATG 2 cut(s) 38, 349
Kzo9I GATC 1 cut(s) 342
LmnI GCTCC 1 cut(s) 466
LpnPI CCDG 2 cut(s) 135, 212
Lsp1109I GCAGC 1 cut(s) 168
MaeII ACGT 3 cut(s) 326, 438, 561
MaeIII GTNAC 4 cut(s) 47, 327, 430, 562
MalI GATC 1 cut(s) 344
MboI GATC 1 cut(s) 342
MboII GAAGA 6 cut(s) 50, 289, 296, 314, 317, 352
MluCI AATT 5 cut(s) 71, 266, 321, 576, 602
MluI ACGCGT 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 40
MnlI CCTC 4 cut(s) 69, 244, 412, 545
MseI TTAA 2 cut(s) 107, 449
MspCI CTTAAG 1 cut(s) 448
Mva1269I GAATGC 1 cut(s) 62
MvnI CGCG 2 cut(s) 112, 506
NdeI CATATG 1 cut(s) 620
NdeII GATC 1 cut(s) 342
NlaIII CATG 2 cut(s) 38, 349
NlaIV GGNNCC 2 cut(s) 27, 533
NmuCI GTSAC 2 cut(s) 47, 562
NspV TTCGAA 1 cut(s) 355
PctI GAATGC 1 cut(s) 62
PkrI GCNGC 2 cut(s) 158, 298
PpuMI RGGWCCY 1 cut(s) 532
Psp5II RGGWCCY 1 cut(s) 532
PspN4I GGNNCC 2 cut(s) 27, 533
PspPI GGNCC 2 cut(s) 26, 532
PspPPI RGGWCCY 1 cut(s) 532
RsaI GTAC 2 cut(s) 498, 625
RsaNI GTAC 2 cut(s) 497, 624
SaqAI TTAA 2 cut(s) 107, 449
SatI GCNGC 2 cut(s) 157, 297
Sau3AI GATC 1 cut(s) 342
Sau96I GGNCC 2 cut(s) 26, 532
SfuI TTCGAA 1 cut(s) 355
SinI GGWCC 2 cut(s) 26, 532
SmlI CTYRAG 3 cut(s) 20, 371, 448
SmoI CTYRAG 3 cut(s) 20, 371, 448
Sse9I AATT 5 cut(s) 71, 266, 321, 576, 602
SsiI CCGC 2 cut(s) 297, 504
SspI AATATT 1 cut(s) 556
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 3 cut(s) 329, 441, 564
TaqI TCGA 1 cut(s) 355
TaqII GACCGA 1 cut(s) 43
TasI AATT 5 cut(s) 71, 266, 321, 576, 602
TatI WGTACW 1 cut(s) 623
TauI GCSGC 1 cut(s) 299
Tru1I TTAA 2 cut(s) 107, 449
Tru9I TTAA 2 cut(s) 107, 449
TseFI GTSAC 2 cut(s) 47, 562
TseI GCWGC 1 cut(s) 156
Tsp45I GTSAC 2 cut(s) 47, 562
TspDTI ATGAA 3 cut(s) 51, 207, 330
TspGWI ACGGA 2 cut(s) 43, 140
Vha464I CTTAAG 1 cut(s) 448
VpaK11BI GGWCC 2 cut(s) 26, 532
XapI RAATTY 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.