MD11G1086200.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0018893
Physical Location & Seq
Forward (+)
7263828 .. 7265687
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1086200.v1.1.491

Sequence Viewer

Length: 792 bp
ATGAGAGGATGTTGTGGTCTAAAATATGTGTTCTCGCATTTAATTGCCCGAGAACTTTTGAATCTTCAAGAGGTAAAGATATCAAAATGTCCGGATATGAAAACTATAGTCAGAATTACAGAGGAAAATGAAGAAGAGGCGATAAAATACATGATTTTGTTTCCGAAACTGAACACATTTGAACTGGTAGACCTGCCTCGTCTCTCAAGTCTTTGTCCAGAGGGATTTACATTTCTATGGTCATCCGCAAAAAATATGCGCGTGGAAAACTGTGAAAAATTGAAGACATTGGGTGCCGTAATTCCACAAAGAAAGAAGTTGGAGAATAACATGAAGAAGGATTCAACAAGTCATGATTTCAGCACTTCCCCAACAAGTTCATCAAATTGGTGCCCCGCTGGATGTGGATGCGCACCATATTCAAGACCAAACGCCCACCGCTCCATTGAAATATTGCCACGTCCAGTTAATTTGGAGGTTACACCAACTAATCTTGAGGACTCAAATGATGATGATAATCTTGAAAATCTTACTGTAAGTGACTGTAATTCAATGGAAGTGATTTTCCAACTAAAGGGACCGAAGAATGAAGAAAGTAATCACTCCATTGAAGCATTCAATAAATTGAGGTCCTTCCAGTTACATAAGTTGCCAAGTTTAACGCGTGTTTGGGAGGTGGGTAGTTCACAACCAATGTTCACAGGAAGCAGTTTTGGAAACTTGAAATCGTTGCAGGTCGATTGTTGCAACCAGTGTTCTAAAAATCGGCCTAGGCGGCCGCCTAGGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

264

Amino Acids

29.84

Weight (kDa)

8.42

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 3 - 97 9.7e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 172 - 251 8e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 412
Acc36I ACCTGC 2 cut(s) 201, 724
AccB1I GGYRCC 2 cut(s) 293, 390
AccBSI CCGCTC 1 cut(s) 441
AccI GTMKAC 1 cut(s) 189
AccII CGCG 2 cut(s) 261, 664
AccIII TCCGGA 1 cut(s) 91
AciI CCGC 5 cut(s) 246, 396, 439, 775, 779
AcoI YGGCCR 1 cut(s) 776
AfiI CCNNNNNNNGG 1 cut(s) 574
AflIII ACRYGT 1 cut(s) 662
AjiI CACGTC 1 cut(s) 461
Alw26I GTCTC 1 cut(s) 206
Ama87I CYCGRG 1 cut(s) 48
Aor13HI TCCGGA 1 cut(s) 91
AoxI GGCC 2 cut(s) 767, 776
AspA2I CCTAGG 2 cut(s) 770, 782
AspLEI GCGC 3 cut(s) 261, 413, 789
AspS9I GGNCC 2 cut(s) 578, 630
AvaI CYCGRG 1 cut(s) 48
AvaII GGWCC 2 cut(s) 578, 630
AvrII CCTAGG 2 cut(s) 770, 782
BaeGI GKGCMC 1 cut(s) 395
BanI GGYRCC 2 cut(s) 293, 390
BbsI GAAGAC 1 cut(s) 290
BceAI ACGGC 1 cut(s) 281
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 3 cut(s) 771, 783, 790
BfmI CTRYAG 1 cut(s) 105
BfoI RGCGCY 1 cut(s) 790
BfuAI ACCTGC 2 cut(s) 201, 724
BglI GCCNNNNNGGC 2 cut(s) 775, 784
BisI GCNGC 2 cut(s) 776, 779
BlnI CCTAGG 2 cut(s) 770, 782
BlsI GCNGC 2 cut(s) 777, 780
Bme18I GGWCC 2 cut(s) 578, 630
BmeT110I CYCGRG 1 cut(s) 48
BmgBI CACGTC 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 578, 630
BmiI GGNNCC 3 cut(s) 295, 392, 579
BmsI GCATC 1 cut(s) 398
BpiI GAAGAC 1 cut(s) 290
BpuEI CTTGAG 2 cut(s) 190, 515
BsaBI GATNNNNATC 1 cut(s) 516
BsaJI CCNNGG 2 cut(s) 770, 782
BsaWI WCCGGW 1 cut(s) 91
Bsc4I CCNNNNNNNGG 1 cut(s) 574
Bse1I ACTGG 4 cut(s) 189, 464, 637, 751
Bse8I GATNNNNATC 1 cut(s) 516
BseAI TCCGGA 1 cut(s) 91
BseDI CCNNGG 2 cut(s) 770, 782
BseGI GGATG 4 cut(s) 14, 242, 407, 413
BseJI GATNNNNATC 1 cut(s) 516
BseLI CCNNNNNNNGG 1 cut(s) 574
BseNI ACTGG 4 cut(s) 189, 464, 637, 751
BseSI GKGCMC 1 cut(s) 395
BseX3I CGGCCG 1 cut(s) 776
Bsh1236I CGCG 2 cut(s) 261, 664
Bsh1285I CGRYCG 1 cut(s) 779
BshFI GGCC 2 cut(s) 769, 778
BshNI GGYRCC 2 cut(s) 293, 390
BsiEI CGRYCG 1 cut(s) 779
BsiHKCI CYCGRG 1 cut(s) 48
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 1 cut(s) 591
BslI CCNNNNNNNGG 1 cut(s) 574
BsmAI GTCTC 1 cut(s) 206
BsmBI CGTCTC 1 cut(s) 206
BsmFI GGGAC 1 cut(s) 591
BsmI GAATGC 1 cut(s) 614
BsnI GGCC 2 cut(s) 769, 778
BsoBI CYCGRG 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 395
Bsp13I TCCGGA 1 cut(s) 91
BspACI CCGC 5 cut(s) 246, 396, 439, 775, 779
BspANI GGCC 2 cut(s) 769, 778
BspEI TCCGGA 1 cut(s) 91
BspFNI CGCG 2 cut(s) 261, 664
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 3 cut(s) 295, 392, 579
BspMI ACCTGC 2 cut(s) 201, 724
BspT107I GGYRCC 2 cut(s) 293, 390
BsrBI CCGCTC 1 cut(s) 441
BsrI ACTGG 4 cut(s) 189, 464, 637, 751
BssECI CCNNGG 2 cut(s) 770, 782
BssT1I CCWWGG 2 cut(s) 770, 782
Bst4CI ACNGT 3 cut(s) 272, 535, 545
Bst6I CTCTTC 1 cut(s) 129
BstF5I GGATG 4 cut(s) 14, 242, 407, 413
BstFNI CGCG 2 cut(s) 261, 664
BstH2I RGCGCY 1 cut(s) 790
BstHHI GCGC 3 cut(s) 261, 413, 789
BstMAI GTCTC 1 cut(s) 206
BstMCI CGRYCG 1 cut(s) 779
BstMWI GCNNNNNNNGC 2 cut(s) 775, 784
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 1 cut(s) 395
BstUI CGCG 2 cut(s) 261, 664
BstV2I GAAGAC 1 cut(s) 290
BstZI CGGCCG 1 cut(s) 776
BsuRI GGCC 2 cut(s) 769, 778
BtrI CACGTC 1 cut(s) 461
BtsCI GGATG 4 cut(s) 14, 242, 407, 413
BtsIMutI CAGTG 1 cut(s) 758
BveI ACCTGC 2 cut(s) 201, 724
CciI TCATGA 1 cut(s) 352
CciNI GCGGCCGC 1 cut(s) 776
CfoI GCGC 3 cut(s) 261, 413, 789
Cfr13I GGNCC 2 cut(s) 578, 630
CviAII CATG 3 cut(s) 151, 331, 353
CviJI RGCY 2 cut(s) 769, 778
CviKI_1 RGCY 2 cut(s) 769, 778
EaeI YGGCCR 1 cut(s) 776
EagI CGGCCG 1 cut(s) 776
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
EclXI CGGCCG 1 cut(s) 776
Eco130I CCWWGG 2 cut(s) 770, 782
Eco32I GATATC 1 cut(s) 81
Eco47I GGWCC 2 cut(s) 578, 630
Eco52I CGGCCG 1 cut(s) 776
Eco88I CYCGRG 1 cut(s) 48
EcoO109I RGGNCCY 1 cut(s) 630
EcoRV GATATC 1 cut(s) 81
EcoT14I CCWWGG 2 cut(s) 770, 782
ErhI CCWWGG 2 cut(s) 770, 782
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 3 cut(s) 154, 334, 356
FaqI GGGAC 1 cut(s) 591
FatI CATG 3 cut(s) 150, 330, 352
FauI CCCGC 1 cut(s) 403
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 2 cut(s) 776, 779
FokI GGATG 4 cut(s) 21, 229, 414, 420
Fsp4HI GCNGC 2 cut(s) 776, 779
FspAI RTGCGCAY 1 cut(s) 412
FspBI CTAG 3 cut(s) 771, 783, 790
FspI TGCGCA 1 cut(s) 412
GlaI GCGC 3 cut(s) 260, 412, 788
GluI GCNGC 2 cut(s) 776, 779
HaeII RGCGCY 1 cut(s) 790
HaeIII GGCC 2 cut(s) 769, 778
HapII CCGG 1 cut(s) 92
HhaI GCGC 3 cut(s) 261, 413, 789
Hin1II CATG 3 cut(s) 154, 334, 356
Hin6I GCGC 3 cut(s) 259, 411, 787
HinP1I GCGC 3 cut(s) 259, 411, 787
HinfI GANTC 3 cut(s) 61, 341, 500
HpaII CCGG 1 cut(s) 92
Hpy166II GTNNAC 3 cut(s) 190, 686, 699
Hpy188I TCNGA 2 cut(s) 113, 165
Hpy188III TCNNGA 7 cut(s) 68, 92, 218, 353, 423, 494, 521
Hpy8I GTNNAC 3 cut(s) 190, 686, 699
HpyAV CCTTC 2 cut(s) 331, 643
HpyCH4III ACNGT 3 cut(s) 272, 535, 545
HpyCH4IV ACGT 1 cut(s) 460
HpyCH4V TGCA 2 cut(s) 733, 747
HpyF10VI GCNNNNNNNGC 2 cut(s) 775, 784
HpySE526I ACGT 1 cut(s) 460
Hsp92II CATG 3 cut(s) 154, 334, 356
HspAI GCGC 3 cut(s) 259, 411, 787
Kpn2I TCCGGA 1 cut(s) 91
LmnI GCTCC 1 cut(s) 446
LweI GCATC 1 cut(s) 398
MaeI CTAG 3 cut(s) 771, 783, 790
MaeII ACGT 1 cut(s) 460
MaeIII GTNAC 3 cut(s) 478, 539, 639
MbiI CCGCTC 1 cut(s) 441
MboII GAAGA 7 cut(s) 56, 143, 146, 295, 346, 595, 602
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 8 cut(s) 42, 114, 278, 300, 385, 469, 547, 623
MluI ACGCGT 1 cut(s) 662
MlyI GAGTC 1 cut(s) 494
MmeI TCCRAC 2 cut(s) 300, 592
MnlI CCTC 9 cut(s) 64, 115, 130, 207, 214, 469, 490, 621, 667
MroI TCCGGA 1 cut(s) 91
MseI TTAA 3 cut(s) 41, 468, 659
MslI CAYNNNNRTG 1 cut(s) 235
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 1 cut(s) 92
Mva1269I GAATGC 1 cut(s) 614
MvnI CGCG 2 cut(s) 261, 664
MwoI GCNNNNNNNGC 2 cut(s) 775, 784
NlaIII CATG 3 cut(s) 154, 334, 356
NlaIV GGNNCC 3 cut(s) 295, 392, 579
NmuCI GTSAC 1 cut(s) 539
NotI GCGGCCGC 1 cut(s) 776
NsbI TGCGCA 1 cut(s) 412
PagI TCATGA 1 cut(s) 352
PctI GAATGC 1 cut(s) 614
PfeI GAWTC 2 cut(s) 61, 341
PkrI GCNGC 2 cut(s) 777, 780
PleI GAGTC 1 cut(s) 494
PpsI GAGTC 1 cut(s) 494
PpuMI RGGWCCY 1 cut(s) 630
Psp5II RGGWCCY 1 cut(s) 630
PspN4I GGNNCC 3 cut(s) 295, 392, 579
PspPI GGNCC 2 cut(s) 578, 630
PspPPI RGGWCCY 1 cut(s) 630
RseI CAYNNNNRTG 1 cut(s) 235
SaqAI TTAA 3 cut(s) 41, 468, 659
SatI GCNGC 2 cut(s) 776, 779
Sau96I GGNCC 2 cut(s) 578, 630
SchI GAGTC 1 cut(s) 494
SduI GDGCHC 1 cut(s) 395
SetI ASST 7 cut(s) 75, 195, 463, 480, 632, 678, 738
SfaNI GCATC 1 cut(s) 398
SfcI CTRYAG 1 cut(s) 105
SfiI GGCCNNNNNGGCC 1 cut(s) 775
SinI GGWCC 2 cut(s) 578, 630
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 2 cut(s) 205, 494
SmoI CTYRAG 2 cut(s) 205, 494
Sse9I AATT 8 cut(s) 42, 114, 278, 300, 385, 469, 547, 623
SsiI CCGC 5 cut(s) 246, 396, 439, 775, 779
SspI AATATT 1 cut(s) 453
SspMI CTAG 3 cut(s) 771, 783, 790
StyI CCWWGG 2 cut(s) 770, 782
TaaI ACNGT 3 cut(s) 272, 535, 545
TaiI ACGT 1 cut(s) 463
TaqI TCGA 1 cut(s) 738
TaqII GACCGA 1 cut(s) 595
TasI AATT 8 cut(s) 42, 114, 278, 300, 385, 469, 547, 623
TauI GCSGC 2 cut(s) 778, 781
TfiI GAWTC 2 cut(s) 61, 341
Tru1I TTAA 3 cut(s) 41, 468, 659
Tru9I TTAA 3 cut(s) 41, 468, 659
TscAI CASTG 1 cut(s) 758
TseFI GTSAC 1 cut(s) 539
Tsp45I GTSAC 1 cut(s) 539
TspDTI ATGAA 5 cut(s) 113, 144, 347, 369, 603
TspRI CASTG 1 cut(s) 758
VpaK11BI GGWCC 2 cut(s) 578, 630
XmaJI CCTAGG 2 cut(s) 770, 782
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 3 cut(s) 771, 783, 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.