MD03G1085400.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010786
Physical Location & Seq
Reverse (-)
6898086 .. 6900171
2086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1085400.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGGCGGAGACTTGCTACGGAGTTGTGAGCGAAAGCGACTCGGCGTCGAATGCCTGCGAGACCAGTTCACGAGCTGCGAGGAGGAGGAGGATGGAGATCCGGCGGTTCAAATTCGTCTCCGGAGTGGCCCCACCGGCGCCGGAGTCACCGGAAGGTTCGGAGAAGCAGAAGAAAGCCGCGTCCCGTACGATGTCGTTGCCTTCTCGGGAGTTGGAAAGCGCCTTGCTGTGCTCCGGTTCTTCGGACGAAGAGAAAATCCCCCCGAGTCGAAAGAAGTCAACGAAGTTCCGAGCGTCCACGTCATCGAACGTTACAGAAGCTTCGAGGGAGATGCTCAGATACGGTGTGTCCTCCGTCTGCGGCCGCCGTCGAGATATGGAGGACGCTGTGTCCGTGCACCCCTCGTTTTGCCGACGCATCCGAGACTCCGCTGCTCAGTTGCATTACTTGGGCGTCTACGACGGCCACGGCTGCTCACATGTGGCGACGAAATGCAGAGAGTGGATGCATGAGCTGGTGAAGGACGAAGTGGAGAGCATGGAAGAGTGGAAGACAGCGATGGAGAGGAGCTTTGTGCGGATGGACAAGGAGGTGGTGGCGTGGGGCCGCGAAGGCGGCATCGGAGTGAGCAATTGCCGTTGTGAGCTTCAAACTCCGGAGTCCGAGGCTGCCGGATCCACCGCTGTCGTCGCAGTCATCGCTCCCGATAAGATCGTCGTGGCTAACTGCGGCGACTCGAGAGCCGTGCTCTGTCGCAACGGCAAGGCCTTTCCTCTCTCTGTTGATCACAAGCCGGATCGCCCCGATGAGTTGAATCGGATCCAGGAGGCGGGTGGGCGGGTCATATACTGGGACGGCCCACGGGTTCTCGGAGTTCTGGCGATGTCAAGAGCCATAGGCGACAACTACTTGAAGCCGTACGTGAGCTGCGTTCCAGAGGTGACGATCACGGACAGGACGGTGGAGGACGAGTGTCTGATCCTGGCGAGCGACGGGCTGTGGGACGTGGTGTCGAACGAAACGGCATGCGGGGTGGCGAGAATGTGTCTGAGAGGGAAACGGACGGCTTCCGCGATGGAGCGCGCGGGGGCGGAGGCGGTGGCGAAGGTGGCGTCGGACAGGGCGTGTTCCGACGCGTCGATACTGCTGACGAAGCTGGCATTGGCCAGGCACAGCGCTGACAACGTGAGCGTTATCGTGGTGAACCTCAGACGCAACACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005801 GO:0005829 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009657 GO:0009658 GO:0009719 GO:0009725 GO:0009737 GO:0009787 GO:0009788 GO:0009791 GO:0009937 GO:0009939 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010029 GO:0010030 GO:0010033 GO:0010035 GO:0010118 GO:0010150 GO:0010154 GO:0010162 GO:0010431 GO:0010646 GO:0010647 GO:0010648 GO:0012505 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0021700 GO:0022414 GO:0022611 GO:0023051 GO:0023056 GO:0023057 GO:0031090 GO:0032501 GO:0032502 GO:0032504 GO:0033106 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048581 GO:0048582 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048838 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051094 GO:0051239 GO:0051240 GO:0051241 GO:0061458 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080050 GO:0090693 GO:0097305 GO:0097438 GO:0098588 GO:0099402 GO:0140096 GO:1900140 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1902039 GO:1905957 GO:1905958 GO:2000026 GO:2000033 GO:2000034 GO:2000241 GO:2000242 GO:2000692
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

408

Amino Acids

44.43

Weight (kDa)

8.1

Isoelectric Point (pI)

55.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 113 - 372 4.9e-63 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010786)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29380 AT5G59220 AT5G59220
fragaria_vesca FvH4_3g37010 FvH4_3g37010
malus_domestica MD03G1085400.v1.1 MD11G1093100.v1.1
prunus_persica Prupe.6G068800_v2.0.a1
pyrus_communis pycom03g06750 pycom11g07870
rosa_chinensis RchiOBHm_Chr5g0066401
rosa_laevigata RLG00000035860
rosa_multiflora Rmu_ssc0000249.1_g000014
rosa_roxburghii Rroxscaffold_1G00014640
rosa_rugosa Rorug05G0378000
rosa_samantha Rh5AG436700 Rh5BG452900 Rh5CG475300 Rh5DG468100
rosa_wichuraiana Rw5G040890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 136
AccI GTMKAC 1 cut(s) 456
AccII CGCG 6 cut(s) 179, 609, 1073, 1083, 1085, 1136
AccIII TCCGGA 2 cut(s) 119, 655
AclI AACGTT 1 cut(s) 309
AclWI GGATC 7 cut(s) 91, 669, 682, 804, 814, 827, 973
AcoI YGGCCR 3 cut(s) 361, 463, 1164
AcsI RAATTY 1 cut(s) 110
AcyI GRCGYC 4 cut(s) 44, 137, 453, 1112
AfaI GTAC 2 cut(s) 187, 920
AfeI AGCGCT 1 cut(s) 1177
AfiI CCNNNNNNNGG 1 cut(s) 829
AflIII ACRYGT 3 cut(s) 478, 1134, 1218
AgsI TTSAA 4 cut(s) 109, 650, 814, 913
AhdI GACNNNNNGTC 3 cut(s) 43, 388, 1009
AjiI CACGTC 2 cut(s) 300, 1006
AjnI CCWGG 3 cut(s) 822, 981, 1166
AjuI GAANNNNNNNTTGG 2 cut(s) 1145, 1177
AluBI AGCT 7 cut(s) 74, 320, 514, 570, 646, 927, 1156
AluI AGCT 7 cut(s) 74, 320, 514, 570, 646, 927, 1156
Alw21I GWGCWC 3 cut(s) 233, 399, 750
Alw26I GTCTC 4 cut(s) 2, 53, 121, 417
Alw44I GTGCAC 1 cut(s) 395
AlwI GGATC 7 cut(s) 91, 669, 682, 804, 814, 827, 973
Ama87I CYCGRG 3 cut(s) 204, 262, 736
Aor13HI TCCGGA 2 cut(s) 119, 655
Aor51HI AGCGCT 1 cut(s) 1177
AoxI GGCC 7 cut(s) 126, 361, 463, 604, 765, 856, 1164
ApaLI GTGCAC 1 cut(s) 395
ApeKI GCWGC 5 cut(s) 74, 431, 471, 668, 927
ApoI RAATTY 1 cut(s) 110
ArsI GACNNNNNNTTYG 2 cut(s) 1097, 1129
AspLEI GCGC 5 cut(s) 139, 221, 1083, 1085, 1178
AspS9I GGNCC 3 cut(s) 127, 604, 857
AsuHPI GGTGA 4 cut(s) 138, 529, 952, 1213
AvaI CYCGRG 3 cut(s) 204, 262, 736
BaeGI GKGCMC 1 cut(s) 399
BalI TGGCCA 1 cut(s) 1166
BamHI GGATCC 2 cut(s) 674, 819
BanI GGYRCC 1 cut(s) 136
BauI CACGAG 1 cut(s) 69
BbsI GAAGAC 1 cut(s) 557
Bbv12I GWGCWC 3 cut(s) 233, 399, 750
BbvI GCAGC 5 cut(s) 61, 418, 458, 655, 914
BccI CCATC 4 cut(s) 85, 553, 574, 1069
BcgI CGANNNNNNTGC 6 cut(s) 178, 212, 313, 347, 727, 761
BciT130I CCWGG 3 cut(s) 824, 983, 1168
BclI TGATCA 1 cut(s) 784
BcoDI GTCTC 4 cut(s) 2, 53, 121, 417
BfoI RGCGCY 3 cut(s) 140, 222, 1179
BglI GCCNNNNNGGC 2 cut(s) 134, 612
Bme1390I CCNGG 3 cut(s) 824, 983, 1168
BmeRI GACNNNNNGTC 3 cut(s) 43, 388, 1009
BmeT110I CYCGRG 3 cut(s) 204, 262, 736
BmgBI CACGTC 2 cut(s) 300, 1006
BmgT120I GGNCC 3 cut(s) 127, 604, 857
BmiI GGNNCC 5 cut(s) 129, 138, 605, 676, 821
BmrFI CCNGG 3 cut(s) 824, 983, 1168
BmrI ACTGGG 1 cut(s) 859
BmsI GCATC 4 cut(s) 321, 426, 495, 627
BmuI ACTGGG 1 cut(s) 859
BoxI GACNNNNGTC 1 cut(s) 972
BpiI GAAGAC 1 cut(s) 557
BplI GAGNNNNNCTC 2 cut(s) 732, 764
BsaAI YACGTR 2 cut(s) 922, 1221
BsaBI GATNNNNATC 1 cut(s) 95
BsaHI GRCGYC 4 cut(s) 44, 137, 453, 1112
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 3 cut(s) 466, 663, 860
BsaWI WCCGGW 4 cut(s) 119, 148, 233, 655
BsaXI ACNNNNNCTCC 2 cut(s) 956, 986
Bsc4I CCNNNNNNNGG 1 cut(s) 829
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 2 cut(s) 63, 854
Bse8I GATNNNNATC 1 cut(s) 95
BseAI TCCGGA 2 cut(s) 119, 655
BseBI CCWGG 3 cut(s) 824, 983, 1168
BseDI CCNNGG 3 cut(s) 466, 663, 860
BseGI GGATG 4 cut(s) 96, 417, 510, 585
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 829
BseMII CTCAG 4 cut(s) 349, 449, 1040, 1222
BseNI ACTGG 2 cut(s) 63, 854
BsePI GCGCGC 1 cut(s) 1081
BseRI GAGGAG 4 cut(s) 94, 97, 100, 580
BseSI GKGCMC 1 cut(s) 399
BseX3I CGGCCG 1 cut(s) 361
BseXI GCAGC 5 cut(s) 61, 418, 458, 655, 914
Bsh1236I CGCG 6 cut(s) 179, 609, 1073, 1083, 1085, 1136
Bsh1285I CGRYCG 1 cut(s) 364
BshFI GGCC 7 cut(s) 128, 363, 465, 606, 767, 858, 1166
BshNI GGYRCC 1 cut(s) 136
BsiEI CGRYCG 1 cut(s) 364
BsiHKAI GWGCWC 3 cut(s) 233, 399, 750
BsiHKCI CYCGRG 3 cut(s) 204, 262, 736
BsiSI CCGG 9 cut(s) 100, 120, 134, 140, 149, 234, 656, 672, 794
BsiWI CGTACG 2 cut(s) 185, 918
BslFI GGGAC 3 cut(s) 166, 866, 1016
BslI CCNNNNNNNGG 1 cut(s) 829
BsmAI GTCTC 4 cut(s) 2, 53, 121, 417
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 3 cut(s) 166, 866, 1016
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 7 cut(s) 128, 363, 465, 606, 767, 858, 1166
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 3 cut(s) 204, 262, 736
Bsp1286I GDGCHC 3 cut(s) 233, 399, 750
Bsp13I TCCGGA 2 cut(s) 119, 655
Bsp143I GATC 8 cut(s) 96, 674, 711, 784, 796, 819, 945, 978
BspANI GGCC 7 cut(s) 128, 363, 465, 606, 767, 858, 1166
BspCNI CTCAG 4 cut(s) 348, 448, 1041, 1221
BspEI TCCGGA 2 cut(s) 119, 655
BspFNI CGCG 6 cut(s) 179, 609, 1073, 1083, 1085, 1136
BspLI GGNNCC 5 cut(s) 129, 138, 605, 676, 821
BspPI GGATC 7 cut(s) 91, 669, 682, 804, 814, 827, 973
BspT107I GGYRCC 1 cut(s) 136
BspTNI GGTCTC 1 cut(s) 53
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 2 cut(s) 63, 854
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 3 cut(s) 466, 663, 860
BssHII GCGCGC 1 cut(s) 1081
BssMI GATC 8 cut(s) 96, 674, 711, 784, 796, 819, 945, 978
BssNI GRCGYC 4 cut(s) 44, 137, 453, 1112
BssSI CACGAG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 69
Bst2UI CCWGG 3 cut(s) 824, 983, 1168
Bst4CI ACNGT 2 cut(s) 344, 961
Bst6I CTCTTC 2 cut(s) 243, 537
BstACI GRCGYC 4 cut(s) 44, 137, 453, 1112
BstBAI YACGTR 2 cut(s) 922, 1221
BstC8I GCNNGC 5 cut(s) 55, 988, 1027, 1083, 1158
BstDEI CTNAG 4 cut(s) 335, 435, 1049, 1208
BstDSI CCRYGG 2 cut(s) 466, 860
BstF5I GGATG 4 cut(s) 96, 417, 510, 585
BstFNI CGCG 6 cut(s) 179, 609, 1073, 1083, 1085, 1136
BstH2I RGCGCY 3 cut(s) 140, 222, 1179
BstHHI GCGC 5 cut(s) 139, 221, 1083, 1085, 1178
BstKTI GATC 8 cut(s) 99, 677, 714, 787, 799, 822, 948, 981
BstMAI GTCTC 4 cut(s) 2, 53, 121, 417
BstMBI GATC 8 cut(s) 96, 674, 711, 784, 796, 819, 945, 978
BstMCI CGRYCG 1 cut(s) 364
BstMWI GCNNNNNNNGC 9 cut(s) 50, 134, 471, 612, 615, 689, 698, 1109, 1153
BstNI CCWGG 3 cut(s) 824, 983, 1168
BstNSI RCATGY 2 cut(s) 482, 1029
BstPAI GACNNNNGTC 1 cut(s) 972
BstSCI CCNGG 3 cut(s) 822, 981, 1166
BstSLI GKGCMC 1 cut(s) 399
BstUI CGCG 6 cut(s) 179, 609, 1073, 1083, 1085, 1136
BstV1I GCAGC 5 cut(s) 61, 418, 458, 655, 914
BstV2I GAAGAC 1 cut(s) 557
BstX2I RGATCY 3 cut(s) 96, 674, 819
BstYI RGATCY 3 cut(s) 96, 674, 819
BstZI CGGCCG 1 cut(s) 361
BsuRI GGCC 7 cut(s) 128, 363, 465, 606, 767, 858, 1166
BtgI CCRYGG 2 cut(s) 466, 860
BtgZI GCGATG 4 cut(s) 572, 682, 896, 1088
BtrI CACGTC 2 cut(s) 300, 1006
BtsCI GGATG 4 cut(s) 96, 417, 510, 585
Cac8I GCNNGC 5 cut(s) 55, 988, 1027, 1083, 1158
CciNI GCGGCCGC 1 cut(s) 361
CfoI GCGC 5 cut(s) 139, 221, 1083, 1085, 1178
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 3 cut(s) 127, 604, 857
CseI GACGC 9 cut(s) 33, 168, 282, 392, 423, 442, 1101, 1125, 1142
Csp6I GTAC 2 cut(s) 186, 919
CviAII CATG 4 cut(s) 479, 509, 538, 1026
CviQI GTAC 2 cut(s) 186, 919
DdeI CTNAG 4 cut(s) 335, 435, 1049, 1208
DinI GGCGCC 1 cut(s) 138
DpnI GATC 8 cut(s) 98, 676, 713, 786, 798, 821, 947, 980
DpnII GATC 8 cut(s) 96, 674, 711, 784, 796, 819, 945, 978
DriI GACNNNNNGTC 3 cut(s) 43, 388, 1009
EaeI YGGCCR 3 cut(s) 361, 463, 1164
EagI CGGCCG 1 cut(s) 361
Eam1104I CTCTTC 2 cut(s) 243, 537
Eam1105I GACNNNNNGTC 3 cut(s) 43, 388, 1009
EarI CTCTTC 2 cut(s) 243, 537
EciI GGCGGA 2 cut(s) 20, 1106
EclXI CGGCCG 1 cut(s) 361
Eco147I AGGCCT 1 cut(s) 767
Eco31I GGTCTC 1 cut(s) 53
Eco47III AGCGCT 1 cut(s) 1177
Eco52I CGGCCG 1 cut(s) 361
Eco88I CYCGRG 3 cut(s) 204, 262, 736
EcoRII CCWGG 3 cut(s) 822, 981, 1166
EcoT22I ATGCAT 1 cut(s) 510
EgeI GGCGCC 1 cut(s) 138
EheI GGCGCC 1 cut(s) 138
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 4 cut(s) 482, 512, 541, 1029
FaiI YATR 8 cut(s) 377, 480, 510, 539, 845, 847, 896, 1027
FaqI GGGAC 3 cut(s) 166, 866, 1016
FatI CATG 4 cut(s) 478, 508, 537, 1025
FauI CCCGC 4 cut(s) 823, 831, 1022, 1078
FbaI TGATCA 1 cut(s) 784
FblI GTMKAC 1 cut(s) 456
FokI GGATG 4 cut(s) 103, 404, 517, 592
GlaI GCGC 5 cut(s) 138, 220, 1082, 1084, 1177
HaeII RGCGCY 3 cut(s) 140, 222, 1179
HaeIII GGCC 7 cut(s) 128, 363, 465, 606, 767, 858, 1166
HapII CCGG 9 cut(s) 100, 120, 134, 140, 149, 234, 656, 672, 794
HgaI GACGC 9 cut(s) 33, 168, 282, 392, 423, 442, 1101, 1125, 1142
HhaI GCGC 5 cut(s) 139, 221, 1083, 1085, 1178
Hin1I GRCGYC 4 cut(s) 44, 137, 453, 1112
Hin1II CATG 4 cut(s) 482, 512, 541, 1029
Hin6I GCGC 5 cut(s) 137, 219, 1081, 1083, 1176
HinP1I GCGC 5 cut(s) 137, 219, 1081, 1083, 1176
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HindIII AAGCTT 1 cut(s) 318
HinfI GANTC 7 cut(s) 38, 143, 265, 425, 659, 734, 814
HpaII CCGG 9 cut(s) 100, 120, 134, 140, 149, 234, 656, 672, 794
HphI GGTGA 4 cut(s) 138, 529, 952, 1213
Hpy166II GTNNAC 6 cut(s) 68, 279, 297, 397, 457, 1204
Hpy188III TCNNGA 9 cut(s) 69, 120, 206, 371, 656, 704, 738, 888, 935
Hpy8I GTNNAC 6 cut(s) 68, 279, 297, 397, 457, 1204
HpyAV CCTTC 5 cut(s) 146, 210, 514, 605, 1099
HpyCH4III ACNGT 2 cut(s) 344, 961
HpyCH4IV ACGT 6 cut(s) 299, 309, 921, 1005, 1185, 1220
HpyCH4V TGCA 4 cut(s) 397, 442, 495, 508
HpyF10VI GCNNNNNNNGC 9 cut(s) 50, 134, 471, 612, 615, 689, 698, 1109, 1153
HpyF3I CTNAG 4 cut(s) 335, 435, 1049, 1208
HpySE526I ACGT 6 cut(s) 299, 309, 921, 1005, 1185, 1220
Hsp92I GRCGYC 4 cut(s) 44, 137, 453, 1112
Hsp92II CATG 4 cut(s) 482, 512, 541, 1029
HspAI GCGC 5 cut(s) 137, 219, 1081, 1083, 1176
KasI GGCGCC 1 cut(s) 136
Kpn2I TCCGGA 2 cut(s) 119, 655
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 8 cut(s) 96, 674, 711, 784, 796, 819, 945, 978
LmnI GCTCC 4 cut(s) 236, 567, 706, 1078
Lsp1109I GCAGC 5 cut(s) 61, 418, 458, 655, 914
LweI GCATC 4 cut(s) 321, 426, 495, 627
MaeII ACGT 6 cut(s) 299, 309, 921, 1005, 1185, 1220
MaeIII GTNAC 3 cut(s) 144, 310, 940
MalI GATC 8 cut(s) 98, 676, 713, 786, 798, 821, 947, 980
MboI GATC 8 cut(s) 96, 674, 711, 784, 796, 819, 945, 978
MboII GAAGA 5 cut(s) 181, 231, 260, 554, 562
MfeI CAATTG 1 cut(s) 631
MflI RGATCY 3 cut(s) 96, 674, 819
MhlI GDGCHC 3 cut(s) 233, 399, 750
MlsI TGGCCA 1 cut(s) 1166
MluCI AATT 2 cut(s) 110, 631
MluI ACGCGT 1 cut(s) 1134
MluNI TGGCCA 1 cut(s) 1166
Mly113I GGCGCC 1 cut(s) 137
MlyI GAGTC 6 cut(s) 32, 152, 274, 419, 668, 728
MmeI TCCRAC 3 cut(s) 192, 1095, 1155
Mox20I TGGCCA 1 cut(s) 1166
Mph1103I ATGCAT 1 cut(s) 510
MroI TCCGGA 2 cut(s) 119, 655
MscI TGGCCA 1 cut(s) 1166
MslI CAYNNNNRTG 1 cut(s) 623
Msp20I TGGCCA 1 cut(s) 1166
MspA1I CMGCKG 2 cut(s) 431, 683
MspI CCGG 9 cut(s) 100, 120, 134, 140, 149, 234, 656, 672, 794
MspR9I CCNGG 3 cut(s) 824, 983, 1168
MunI CAATTG 1 cut(s) 631
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 3 cut(s) 824, 983, 1168
MvnI CGCG 6 cut(s) 179, 609, 1073, 1083, 1085, 1136
MwoI GCNNNNNNNGC 9 cut(s) 50, 134, 471, 612, 615, 689, 698, 1109, 1153
NarI GGCGCC 1 cut(s) 137
NdeII GATC 8 cut(s) 96, 674, 711, 784, 796, 819, 945, 978
NlaIII CATG 4 cut(s) 482, 512, 541, 1029
NlaIV GGNNCC 5 cut(s) 129, 138, 605, 676, 821
NmeAIII GCCGAG 1 cut(s) 20
NmuCI GTSAC 2 cut(s) 144, 940
NotI GCGGCCGC 1 cut(s) 361
NsiI ATGCAT 1 cut(s) 510
NspI RCATGY 2 cut(s) 482, 1029
PaeI GCATGC 1 cut(s) 1029
PaeR7I CTCGAG 1 cut(s) 736
PauI GCGCGC 1 cut(s) 1081
PceI AGGCCT 1 cut(s) 767
PciI ACATGT 1 cut(s) 478
PcsI WCGNNNNNNNCGW 4 cut(s) 390, 927, 1070, 1121
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 1 cut(s) 814
Pfl23II CGTACG 2 cut(s) 185, 918
PfoI TCCNGGA 1 cut(s) 822
PleI GAGTC 6 cut(s) 32, 151, 273, 419, 667, 728
PluTI GGCGCC 1 cut(s) 140
PpsI GAGTC 6 cut(s) 32, 151, 273, 419, 667, 728
Ppu21I YACGTR 2 cut(s) 922, 1221
PscI ACATGT 1 cut(s) 478
PshAI GACNNNNGTC 1 cut(s) 972
Psp1406I AACGTT 1 cut(s) 309
Psp6I CCWGG 3 cut(s) 822, 981, 1166
PspGI CCWGG 3 cut(s) 822, 981, 1166
PspLI CGTACG 2 cut(s) 185, 918
PspN4I GGNNCC 5 cut(s) 129, 138, 605, 676, 821
PspPI GGNCC 3 cut(s) 127, 604, 857
PsuI RGATCY 3 cut(s) 96, 674, 819
PteI GCGCGC 1 cut(s) 1081
RsaI GTAC 2 cut(s) 187, 920
RsaNI GTAC 2 cut(s) 186, 919
RseI CAYNNNNRTG 1 cut(s) 623
Sau3AI GATC 8 cut(s) 96, 674, 711, 784, 796, 819, 945, 978
Sau96I GGNCC 3 cut(s) 127, 604, 857
SchI GAGTC 6 cut(s) 32, 152, 274, 419, 668, 728
ScrFI CCNGG 3 cut(s) 824, 983, 1168
SduI GDGCHC 3 cut(s) 233, 399, 750
SfaNI GCATC 4 cut(s) 321, 426, 495, 627
SfoI GGCGCC 1 cut(s) 138
Sfr274I CTCGAG 1 cut(s) 736
SgrAI CRCCGGYG 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 736
SmiMI CAYNNNNRTG 1 cut(s) 623
SmlI CTYRAG 1 cut(s) 736
SmoI CTYRAG 1 cut(s) 736
SphI GCATGC 1 cut(s) 1029
Sse9I AATT 2 cut(s) 110, 631
SseBI AGGCCT 1 cut(s) 767
SspDI GGCGCC 1 cut(s) 136
StuI AGGCCT 1 cut(s) 767
StyD4I CCNGG 3 cut(s) 822, 981, 1166
TaaI ACNGT 2 cut(s) 344, 961
TaiI ACGT 6 cut(s) 302, 312, 924, 1008, 1188, 1223
TaqI TCGA 8 cut(s) 47, 268, 305, 323, 370, 737, 1013, 1139
TasI AATT 2 cut(s) 110, 631
TauI GCSGC 6 cut(s) 179, 363, 366, 609, 618, 732
TfiI GAWTC 1 cut(s) 814
TseFI GTSAC 2 cut(s) 144, 940
TseI GCWGC 5 cut(s) 74, 431, 471, 668, 927
Tsp45I GTSAC 2 cut(s) 144, 940
TspGWI ACGGA 5 cut(s) 33, 343, 382, 965, 1075
VneI GTGCAC 1 cut(s) 395
XapI RAATTY 1 cut(s) 110
XceI RCATGY 2 cut(s) 482, 1029
XcmI CCANNNNNNNNNTGG 1 cut(s) 830
XhoI CTCGAG 1 cut(s) 736
XmiI GTMKAC 1 cut(s) 456
Zsp2I ATGCAT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.