Rh5AG436700

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010786
Physical Location & Seq
Forward (+)
75061406 .. 75062971
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG436700.1

Sequence Viewer

Length: 1203 bp
ATGGCGGAGATTTGTTACGGGGTTTTGAGCGAGAACGAGGCGTCGAGCGCGTGCGAGACGAGCTCACGGGCCGCGAGGCGGAGGAGGATGGAGATCAGGCGGTTCAAGTTCGTCTCCGGCGTGGCGGCCCCGGCCGAGTCGACGGATAATGCCGAGAAGCGAAAGAGACTAGAGTGTCGTACTGTTTCTCGGGGCATGGAGAAGTGCGGTGACGAAGAGAAGGGTAATCTGGTCAAAATTGAAAGCGAGGAGGGGGTTTCGAGTCTAACGATTTCCTCCAAGTGTCCGTCCACGTCAGCACCTGTACTGAATCCTGACGCCAAGTTCGGAATGGTTTCCGTTTGTGGAAGACGACGAGATATGGAAGACGCCGTCTCTATCCACCCCTCCTTTTACCGCGGTGACCAAGAAACTGCCAACAATCCTCACTTCTACGCCGTGTACGACGGCCACGGCTGCTCTCATGTGGCGACGAGGTGTAGGGAGCGGTTCCATGAGGTGGTGAAGGAGGAGATAGAAAACAAGGCTCTTCATGAGTGGCAGAGCGCGTTGGAGAGGAGCTTCACGCGCATGGACATGGAGGTGACTGCGTGGACCCAAAGCACCGTCGCCGGAGCCAACTGTCGGTGCGAGCTTCAAATGCCGGAGTGCGAGGCCGTCGGATCCACCGCCGTTGTCGCTATTGTCACGCCCGATAAGATTATCGTCGCTAACTGTGGTGATTCCAGAGCCGTCCTTTGCCGTGACGGCAAGGCCGTACCTCTTTCCTCCGATCACAAGCCGGATCGTCCAGACGAGCTGAACCGGATCGAGGAGGCGGGTGGCCGGGTTATATATTGGGACGGTCCACGGGTTCTCGGAGTTCTGGCCATGTCAAGAGCCATAGGTGACAACTATTTGAAACCGTACGTGAGCTGCGAGCCGGAGGTGACGATAACGGATCGGACGGCGGAGGACGACTGTCTGATTCTGGCGAGCGACGGCCTGTGGGACGTGGTGTCGAACGACACGGCGTGTGGGGTGGCGCTAATGTGCCTGAAAGGGAAAGCGCACGCGCCTGCGATGGAGAGTGAGGGATCGGAGGTGGAGAACTCCGACAGGGCGTGTTCCGACGCGTCGACGCTGCTGACGAAGCTTGCGTTGGCGAGGCACAGCACTGACAACGTTAGCGTGGTTGTGGTCAATCTTAAGAGCAACACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005801 GO:0005829 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009657 GO:0009658 GO:0009719 GO:0009725 GO:0009737 GO:0009787 GO:0009788 GO:0009791 GO:0009937 GO:0009939 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010029 GO:0010030 GO:0010033 GO:0010035 GO:0010118 GO:0010150 GO:0010154 GO:0010162 GO:0010431 GO:0010646 GO:0010647 GO:0010648 GO:0012505 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0021700 GO:0022414 GO:0022611 GO:0023051 GO:0023056 GO:0023057 GO:0031090 GO:0032501 GO:0032502 GO:0032504 GO:0033106 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048581 GO:0048582 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048838 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051094 GO:0051239 GO:0051240 GO:0051241 GO:0061458 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080050 GO:0090693 GO:0097305 GO:0097438 GO:0098588 GO:0099402 GO:0140096 GO:1900140 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1902039 GO:1905957 GO:1905958 GO:2000026 GO:2000033 GO:2000034 GO:2000241 GO:2000242 GO:2000692
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

400

Amino Acids

43.68

Weight (kDa)

5.32

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 108 - 389 3.8e-66 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010786)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29380 AT5G59220 AT5G59220
fragaria_vesca FvH4_3g37010 FvH4_3g37010
malus_domestica MD03G1085400.v1.1 MD11G1093100.v1.1
prunus_persica Prupe.6G068800_v2.0.a1
pyrus_communis pycom03g06750 pycom11g07870
rosa_chinensis RchiOBHm_Chr5g0066401
rosa_laevigata RLG00000035860
rosa_multiflora Rmu_ssc0000249.1_g000014
rosa_roxburghii Rroxscaffold_1G00014640
rosa_rugosa Rorug05G0378000
rosa_samantha Rh5AG436700 Rh5BG452900 Rh5CG475300 Rh5DG468100
rosa_wichuraiana Rw5G040890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 174
AccB7I CCANNNNNTGG 1 cut(s) 499
AccBSI CCGCTC 1 cut(s) 487
AccI GTMKAC 2 cut(s) 140, 1118
AccII CGCG 7 cut(s) 50, 74, 399, 548, 568, 1055, 1115
AclI AACGTT 1 cut(s) 1164
AclWI GGATC 6 cut(s) 657, 670, 792, 815, 948, 1084
AcoI YGGCCR 4 cut(s) 132, 448, 823, 867
AcyI GRCGYC 3 cut(s) 41, 318, 369
AdeI CACNNNGTG 1 cut(s) 1014
AfaI GTAC 5 cut(s) 181, 306, 443, 759, 908
AfiI CCNNNNNNNGG 4 cut(s) 78, 499, 624, 811
AflII CTTAAG 1 cut(s) 1187
AflIII ACRYGT 2 cut(s) 1113, 1197
AgsI TTSAA 4 cut(s) 106, 242, 638, 901
AhdI GACNNNNNGTC 1 cut(s) 997
AjiI CACGTC 2 cut(s) 294, 994
AjuI GAANNNNNNNTTGG 2 cut(s) 1124, 1156
AluBI AGCT 6 cut(s) 63, 561, 634, 799, 915, 1135
AluI AGCT 6 cut(s) 63, 561, 634, 799, 915, 1135
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 4 cut(s) 50, 118, 160, 379
AlwI GGATC 6 cut(s) 657, 670, 792, 815, 948, 1084
AlwNI CAGNNNCTG 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 189
AoxI GGCC 9 cut(s) 69, 126, 132, 448, 654, 753, 823, 867, 982
ApeKI GCWGC 3 cut(s) 456, 915, 1123
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
Asp700I GAANNNNTTC 1 cut(s) 334
AspLEI GCGC 6 cut(s) 50, 548, 570, 1027, 1051, 1057
AspS9I GGNCC 4 cut(s) 69, 127, 594, 845
AsuC2I CCSGG 2 cut(s) 131, 827
AsuHPI GGTGA 7 cut(s) 221, 413, 514, 595, 731, 899, 940
AvaI CYCGRG 1 cut(s) 189
AvaII GGWCC 2 cut(s) 594, 845
BalI TGGCCA 1 cut(s) 869
BamHI GGATCC 1 cut(s) 662
BanII GRGCYC 1 cut(s) 65
BbsI GAAGAC 2 cut(s) 355, 372
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 3 cut(s) 443, 902, 1110
BccI CCATC 2 cut(s) 82, 1057
BcnI CCSGG 2 cut(s) 131, 827
BcoDI GTCTC 4 cut(s) 50, 118, 160, 379
BfaI CTAG 1 cut(s) 170
BfoI RGCGCY 1 cut(s) 1028
BfrI CTTAAG 1 cut(s) 1187
BglI GCCNNNNNGGC 1 cut(s) 747
BisI GCNGC 5 cut(s) 72, 126, 457, 916, 1124
BlsI GCNGC 5 cut(s) 73, 127, 458, 917, 1125
Bme1390I CCNGG 2 cut(s) 131, 827
Bme18I GGWCC 2 cut(s) 594, 845
BmeRI GACNNNNNGTC 1 cut(s) 997
BmeT110I CYCGRG 1 cut(s) 189
BmgBI CACGTC 2 cut(s) 294, 994
BmgT120I GGNCC 4 cut(s) 69, 127, 594, 845
BmiI GGNNCC 5 cut(s) 129, 491, 596, 616, 664
BmrFI CCNGG 2 cut(s) 131, 827
BoxI GACNNNNGTC 1 cut(s) 960
BpiI GAAGAC 2 cut(s) 355, 372
BplI GAGNNNNNCTC 2 cut(s) 47, 79
BpuMI CCSGG 2 cut(s) 131, 827
BsaAI YACGTR 2 cut(s) 910, 1200
BsaBI GATNNNNATC 1 cut(s) 92
BsaHI GRCGYC 3 cut(s) 41, 318, 369
BsaJI CCNNGG 4 cut(s) 129, 397, 451, 848
BsaWI WCCGGW 1 cut(s) 804
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 499, 624, 811
Bse8I GATNNNNATC 1 cut(s) 92
BseDI CCNNGG 4 cut(s) 129, 397, 451, 848
BseGI GGATG 1 cut(s) 93
BseJI GATNNNNATC 1 cut(s) 92
BseLI CCNNNNNNNGG 4 cut(s) 78, 499, 624, 811
BseRI GAGGAG 5 cut(s) 97, 263, 524, 571, 827
BseX3I CGGCCG 1 cut(s) 132
BseXI GCAGC 3 cut(s) 443, 902, 1110
Bsh1236I CGCG 7 cut(s) 50, 74, 399, 548, 568, 1055, 1115
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 9 cut(s) 71, 128, 134, 450, 656, 755, 825, 869, 984
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 65
BsiHKCI CYCGRG 1 cut(s) 189
BsiSI CCGG 8 cut(s) 117, 131, 612, 644, 782, 805, 826, 923
BsiWI CGTACG 1 cut(s) 906
BslFI GGGAC 2 cut(s) 854, 1004
BslI CCNNNNNNNGG 4 cut(s) 78, 499, 624, 811
BsmAI GTCTC 4 cut(s) 50, 118, 160, 379
BsmBI CGTCTC 3 cut(s) 50, 118, 379
BsmFI GGGAC 2 cut(s) 854, 1004
BsnI GGCC 9 cut(s) 71, 128, 134, 450, 656, 755, 825, 869, 984
BsoBI CYCGRG 1 cut(s) 189
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 7 cut(s) 93, 662, 772, 784, 807, 940, 1076
BspANI GGCC 9 cut(s) 71, 128, 134, 450, 656, 755, 825, 869, 984
BspFNI CGCG 7 cut(s) 50, 74, 399, 548, 568, 1055, 1115
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 5 cut(s) 129, 491, 596, 616, 664
BspPI GGATC 6 cut(s) 657, 670, 792, 815, 948, 1084
BspQI GCTCTTC 1 cut(s) 534
BspTI CTTAAG 1 cut(s) 1187
BsrBI CCGCTC 1 cut(s) 487
BssECI CCNNGG 4 cut(s) 129, 397, 451, 848
BssMI GATC 7 cut(s) 93, 662, 772, 784, 807, 940, 1076
BssNI GRCGYC 3 cut(s) 41, 318, 369
Bst4CI ACNGT 7 cut(s) 184, 607, 623, 716, 845, 906, 962
Bst6I CTCTTC 2 cut(s) 210, 534
BstACI GRCGYC 3 cut(s) 41, 318, 369
BstAFI CTTAAG 1 cut(s) 1187
BstBAI YACGTR 2 cut(s) 910, 1200
BstC8I GCNNGC 7 cut(s) 52, 632, 920, 976, 1053, 1059, 1137
BstDSI CCRYGG 3 cut(s) 397, 451, 848
BstEII GGTNACC 1 cut(s) 401
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 7 cut(s) 50, 74, 399, 548, 568, 1055, 1115
BstH2I RGCGCY 1 cut(s) 1028
BstHHI GCGC 6 cut(s) 50, 548, 570, 1027, 1051, 1057
BstKTI GATC 7 cut(s) 96, 665, 775, 787, 810, 943, 1079
BstMAI GTCTC 4 cut(s) 50, 118, 160, 379
BstMBI GATC 7 cut(s) 93, 662, 772, 784, 807, 940, 1076
BstMCI CGRYCG 1 cut(s) 135
BstMWI GCNNNNNNNGC 9 cut(s) 47, 60, 131, 456, 567, 640, 677, 747, 1132
BstPAI GACNNNNGTC 1 cut(s) 960
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 2 cut(s) 129, 825
BstUI CGCG 7 cut(s) 50, 74, 399, 548, 568, 1055, 1115
BstV1I GCAGC 3 cut(s) 443, 902, 1110
BstV2I GAAGAC 2 cut(s) 355, 372
BstX2I RGATCY 1 cut(s) 662
BstYI RGATCY 1 cut(s) 662
BstZI CGGCCG 1 cut(s) 132
BsuRI GGCC 9 cut(s) 71, 128, 134, 450, 656, 755, 825, 869, 984
BtgI CCRYGG 3 cut(s) 397, 451, 848
BtgZI GCGATG 1 cut(s) 1076
BtrI CACGTC 2 cut(s) 294, 994
BtsCI GGATG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 1155
Cac8I GCNNGC 7 cut(s) 52, 632, 920, 976, 1053, 1059, 1137
CaiI CAGNNNCTG 1 cut(s) 302
CciI TCATGA 1 cut(s) 532
CfoI GCGC 6 cut(s) 50, 548, 570, 1027, 1051, 1057
Cfr13I GGNCC 4 cut(s) 69, 127, 594, 845
Cfr42I CCGCGG 1 cut(s) 400
CseI GACGC 6 cut(s) 30, 326, 377, 1104, 1121, 1129
Csp6I GTAC 5 cut(s) 180, 305, 442, 758, 907
CspCI CAANNNNNGTGG 2 cut(s) 655, 690
CviAII CATG 7 cut(s) 196, 464, 494, 533, 571, 577, 871
CviQI GTAC 5 cut(s) 180, 305, 442, 758, 907
DpnI GATC 7 cut(s) 95, 664, 774, 786, 809, 942, 1078
DpnII GATC 7 cut(s) 93, 662, 772, 784, 807, 940, 1076
DraIII CACNNNGTG 1 cut(s) 1014
DrdI GACNNNNNNGTC 1 cut(s) 174
DriI GACNNNNNGTC 1 cut(s) 997
DseDI GACNNNNNNGTC 1 cut(s) 174
EaeI YGGCCR 4 cut(s) 132, 448, 823, 867
EagI CGGCCG 1 cut(s) 132
Eam1104I CTCTTC 2 cut(s) 210, 534
Eam1105I GACNNNNNGTC 1 cut(s) 997
EarI CTCTTC 2 cut(s) 210, 534
EciI GGCGGA 3 cut(s) 20, 94, 965
Ecl136II GAGCTC 1 cut(s) 63
EclXI CGGCCG 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 65
Eco47I GGWCC 2 cut(s) 594, 845
Eco52I CGGCCG 1 cut(s) 132
Eco53kI GAGCTC 1 cut(s) 63
Eco88I CYCGRG 1 cut(s) 189
Eco91I GGTNACC 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 63
EcoO65I GGTNACC 1 cut(s) 401
EcoT38I GRGCYC 1 cut(s) 65
Esp3I CGTCTC 3 cut(s) 50, 118, 379
FaeI CATG 7 cut(s) 199, 467, 497, 536, 574, 580, 874
FaqI GGGAC 2 cut(s) 854, 1004
FatI CATG 7 cut(s) 195, 463, 493, 532, 570, 576, 870
FauI CCCGC 1 cut(s) 811
FblI GTMKAC 2 cut(s) 140, 1118
Fnu4HI GCNGC 5 cut(s) 72, 126, 457, 916, 1124
FokI GGATG 1 cut(s) 100
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 5 cut(s) 72, 126, 457, 916, 1124
FspBI CTAG 1 cut(s) 170
GlaI GCGC 6 cut(s) 49, 547, 569, 1026, 1050, 1056
GluI GCNGC 5 cut(s) 72, 126, 457, 916, 1124
HaeII RGCGCY 1 cut(s) 1028
HaeIII GGCC 9 cut(s) 71, 128, 134, 450, 656, 755, 825, 869, 984
HapII CCGG 8 cut(s) 117, 131, 612, 644, 782, 805, 826, 923
HgaI GACGC 6 cut(s) 30, 326, 377, 1104, 1121, 1129
HhaI GCGC 6 cut(s) 50, 548, 570, 1027, 1051, 1057
Hin1I GRCGYC 3 cut(s) 41, 318, 369
Hin1II CATG 7 cut(s) 199, 467, 497, 536, 574, 580, 874
Hin6I GCGC 6 cut(s) 48, 546, 568, 1025, 1049, 1055
HinP1I GCGC 6 cut(s) 48, 546, 568, 1025, 1049, 1055
HincII GTYRAC 2 cut(s) 141, 1119
HindII GTYRAC 2 cut(s) 141, 1119
HindIII AAGCTT 1 cut(s) 1133
HinfI GANTC 5 cut(s) 137, 262, 310, 722, 967
HpaII CCGG 8 cut(s) 117, 131, 612, 644, 782, 805, 826, 923
HphI GGTGA 7 cut(s) 221, 413, 514, 595, 731, 899, 940
Hpy166II GTNNAC 6 cut(s) 141, 291, 442, 594, 848, 1119
Hpy188I TCNGA 9 cut(s) 329, 662, 772, 860, 945, 966, 1081, 1096, 1111
Hpy188III TCNNGA 5 cut(s) 314, 533, 726, 791, 876
Hpy8I GTNNAC 6 cut(s) 141, 291, 442, 594, 848, 1119
HpyAV CCTTC 2 cut(s) 214, 499
HpyCH4III ACNGT 7 cut(s) 184, 607, 623, 716, 845, 906, 962
HpyCH4IV ACGT 5 cut(s) 293, 909, 993, 1164, 1199
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 60, 131, 456, 567, 640, 677, 747, 1132
HpySE526I ACGT 5 cut(s) 293, 909, 993, 1164, 1199
Hsp92I GRCGYC 3 cut(s) 41, 318, 369
Hsp92II CATG 7 cut(s) 199, 467, 497, 536, 574, 580, 874
HspAI GCGC 6 cut(s) 48, 546, 568, 1025, 1049, 1055
KspI CCGCGG 1 cut(s) 400
Kzo9I GATC 7 cut(s) 93, 662, 772, 784, 807, 940, 1076
LguI GCTCTTC 1 cut(s) 534
LmnI GCTCC 3 cut(s) 484, 558, 614
Lsp1109I GCAGC 3 cut(s) 443, 902, 1110
MaeI CTAG 1 cut(s) 170
MaeII ACGT 5 cut(s) 293, 909, 993, 1164, 1199
MaeIII GTNAC 8 cut(s) 14, 209, 401, 583, 685, 743, 887, 928
MalI GATC 7 cut(s) 95, 664, 774, 786, 809, 942, 1078
MbiI CCGCTC 1 cut(s) 487
MboI GATC 7 cut(s) 93, 662, 772, 784, 807, 940, 1076
MboII GAAGA 4 cut(s) 227, 360, 377, 521
MflI RGATCY 1 cut(s) 662
MhlI GDGCHC 1 cut(s) 65
MlsI TGGCCA 1 cut(s) 869
MluCI AATT 1 cut(s) 237
MluI ACGCGT 1 cut(s) 1113
MluNI TGGCCA 1 cut(s) 869
MlyI GAGTC 2 cut(s) 146, 271
MmeI TCCRAC 4 cut(s) 531, 640, 1119, 1134
Mox20I TGGCCA 1 cut(s) 869
MroXI GAANNNNTTC 1 cut(s) 334
MscI TGGCCA 1 cut(s) 869
MseI TTAA 1 cut(s) 1188
MslI CAYNNNNRTG 3 cut(s) 569, 575, 581
Msp20I TGGCCA 1 cut(s) 869
MspA1I CMGCKG 1 cut(s) 399
MspCI CTTAAG 1 cut(s) 1187
MspI CCGG 8 cut(s) 117, 131, 612, 644, 782, 805, 826, 923
MspR9I CCNGG 2 cut(s) 131, 827
MvnI CGCG 7 cut(s) 50, 74, 399, 548, 568, 1055, 1115
MwoI GCNNNNNNNGC 9 cut(s) 47, 60, 131, 456, 567, 640, 677, 747, 1132
NciI CCSGG 2 cut(s) 131, 827
NdeII GATC 7 cut(s) 93, 662, 772, 784, 807, 940, 1076
NlaIII CATG 7 cut(s) 199, 467, 497, 536, 574, 580, 874
NlaIV GGNNCC 5 cut(s) 129, 491, 596, 616, 664
NmeAIII GCCGAG 2 cut(s) 160, 178
NmuCI GTSAC 7 cut(s) 209, 401, 583, 685, 743, 887, 928
PagI TCATGA 1 cut(s) 532
PciSI GCTCTTC 1 cut(s) 534
PcsI WCGNNNNNNNCGW 7 cut(s) 117, 266, 441, 753, 915, 1127, 1136
PdmI GAANNNNTTC 1 cut(s) 334
PfeI GAWTC 3 cut(s) 310, 722, 967
Pfl23II CGTACG 1 cut(s) 906
PflFI GACNNNGTC 1 cut(s) 371
PflMI CCANNNNNTGG 1 cut(s) 499
PkrI GCNGC 5 cut(s) 73, 127, 458, 917, 1125
PleI GAGTC 2 cut(s) 145, 270
PpsI GAGTC 2 cut(s) 145, 270
Ppu21I YACGTR 2 cut(s) 910, 1200
PshAI GACNNNNGTC 1 cut(s) 960
Psp124BI GAGCTC 1 cut(s) 65
Psp1406I AACGTT 1 cut(s) 1164
PspEI GGTNACC 1 cut(s) 401
PspLI CGTACG 1 cut(s) 906
PspN4I GGNNCC 5 cut(s) 129, 491, 596, 616, 664
PspPI GGNCC 4 cut(s) 69, 127, 594, 845
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 1 cut(s) 662
PsyI GACNNNGTC 1 cut(s) 371
RsaI GTAC 5 cut(s) 181, 306, 443, 759, 908
RsaNI GTAC 5 cut(s) 180, 305, 442, 758, 907
RseI CAYNNNNRTG 3 cut(s) 569, 575, 581
SacI GAGCTC 1 cut(s) 65
SacII CCGCGG 1 cut(s) 400
SalI GTCGAC 2 cut(s) 139, 1117
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 1 cut(s) 1188
SatI GCNGC 5 cut(s) 72, 126, 457, 916, 1124
Sau3AI GATC 7 cut(s) 93, 662, 772, 784, 807, 940, 1076
Sau96I GGNCC 4 cut(s) 69, 127, 594, 845
SchI GAGTC 2 cut(s) 146, 271
ScrFI CCNGG 2 cut(s) 131, 827
SduI GDGCHC 1 cut(s) 65
Sfr303I CCGCGG 1 cut(s) 400
SgrBI CCGCGG 1 cut(s) 400
SgrDI CGTCGACG 1 cut(s) 1117
SinI GGWCC 2 cut(s) 594, 845
SmiMI CAYNNNNRTG 3 cut(s) 569, 575, 581
SmlI CTYRAG 1 cut(s) 1187
SmoI CTYRAG 1 cut(s) 1187
Sse9I AATT 1 cut(s) 237
SspMI CTAG 1 cut(s) 170
SstI GAGCTC 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 129, 825
TaaI ACNGT 7 cut(s) 184, 607, 623, 716, 845, 906, 962
TaiI ACGT 5 cut(s) 296, 912, 996, 1167, 1202
TaqI TCGA 6 cut(s) 44, 140, 260, 810, 1001, 1118
TasI AATT 1 cut(s) 237
TatI WGTACW 1 cut(s) 304
TauI GCSGC 2 cut(s) 74, 128
TfiI GAWTC 3 cut(s) 310, 722, 967
Tru1I TTAA 1 cut(s) 1188
Tru9I TTAA 1 cut(s) 1188
TscAI CASTG 1 cut(s) 1162
TseFI GTSAC 7 cut(s) 209, 401, 583, 685, 743, 887, 928
TseI GCWGC 3 cut(s) 456, 915, 1123
Tsp45I GTSAC 7 cut(s) 209, 401, 583, 685, 743, 887, 928
TspDTI ATGAA 1 cut(s) 521
TspGWI ACGGA 4 cut(s) 158, 276, 328, 953
TspRI CASTG 1 cut(s) 1162
Tth111I GACNNNGTC 1 cut(s) 371
Van91I CCANNNNNTGG 1 cut(s) 499
Vha464I CTTAAG 1 cut(s) 1187
VpaK11BI GGWCC 2 cut(s) 594, 845
XcmI CCANNNNNNNNNTGG 1 cut(s) 328
XmiI GTMKAC 2 cut(s) 140, 1118
XmnI GAANNNNTTC 1 cut(s) 334
XspI CTAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.