MD03G1094800.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010729
Physical Location & Seq
Forward (+)
8026966 .. 8029732
2767 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1094800.v1.1.491

Sequence Viewer

Length: 2169 bp
ATGGGGAACGGCTTCGGGAAAGTAAGCGAGTGCTTCACCGGCGTCAAGGAACGCCGCCGTCACGGCAGACAAGACGTGTCAGTTTTACCATCGGACCAATACGACGAGGGACTCGGGCACTCCTTCTGCTACGTCCGACCCGACCCTACTTGGCTCGCATCCTCCAAAGTCCACTCCGAAGAAGCAACCACTTTCAGAACTATCTCCGGTGCTTCCGTCAGCGCCAATACTTCCACCCCTCTCTCCACCTCCCTACTCGATCTTTACTCCTACAACAGCATCGATCGAGCGGCGGCGTTCGAGAGCTCCACCTCTTTCGCTTCGATTCCACTGCAACCCATCCCGAGAAATCTGATCAATTCAGGTCCGATTTCGGCTAATTTTGGAGCTGGGGTTCCCGGGTCGGGTCCTCTGGAAAGAGGGTTCCTTTCGGGTCCTATCGAGCGCGGGTTCATGTCGGGTCCACTCGAGCGGGGCATCTTCTCGGGTCCGATGGAAAAGGGTTACTCCGAGCAGTTGCCCCGGAGCTTCTCTATCGGCGGTCTCGGATTTAAATCGAGATCCCGGAAGGGGAAATTGATTGGGGCTTTACGGCGGGCGATATCGAAGACGGTATACCGGGGTCAAAACGCCATCGTTTTGCCAATAAAAAGCCGAGTCGTTTCGGTGAAGGAGGATTGGGTTGTCGGCGGCGGGCAGGAGAAGATTAATCACAACGAGAATTTGACCGTTAGCAGCATGAATTTCAGTAGCGAGGGAAGTTTGGAAGACGACGAGTCGTTGGGGAGTCAGAATCTCCACTGGGCGCAGGGGAAGGCAGGGGAGGATCGGGTGCACGTCGTCGTTTCGGAGGAGGACGGGTGGGTTTTCGTCGGGATTTACGACGGATTCAATGGCCCGGATGCACCGGATTACTTGTTGTCTAATTTGTACACGGCTGTGAACAAAGAGCTTAAAGGGTTGTTGTGGGATGAGAAGGCGGAATCGGGTGCGGTGGCCGCCCCTGCAACCTCCCCTGTTCGCTCCGAAGATTCGAATTCGGACAGTGATGGTGACTTGGGAAGGAATCCGGTGGTGGATGAGCAAGAGAATTATCCTTGTGCAAGTGGGGAGGGGAATTTCGATTCGAATTCGAGGAGAAAAGAAGGGAGGAATTTGAAAACAAAGTACCGCGGCACGGCGAGGAAATGGGAGGATAATCAGAGGAGGTGGAGGTGTGAGTGGGATAGAGAGAGAGTGGAGCTTGATAGGAGGTTGAAGGAACAGATGAGTAAGTTTTCGGGCTCCGATGCATCCACCGGAGCTTCTTGTGCGAATCACTCGGATGTTTTGAAGGCTCTGTCTGAAGCCTTGAGAAAAACAGAGGAGGCATATTTGGAAGTTGCTGATGAGATGCTCTTGGAAAATCCTGAATTGGCTCTGATGGGTTCTTGTGTTCTTGTGATGCTGATGAAGGGTGAGGATGTTTATGTGATGAATGTGGGCGATAGTCGCGTGGTTTTGGCTCAGAAGGCGGAACCCGATTATTGGTTAGGGAAGATTAGGCAGGATTTGGAGAGGATTAATGAGGAAACTTTGAATGATATGGAAGGATGTGACGGCGAGAGAGACAATGTGATTCCCAATTTGACTGCTTTTCAGCTGACCACGGATCACAGCACAAGTGAAGAAGAGGAAGTTCAGAGAATCCGAAATGAACACCCGAAAGATGCTTGCGCTGTGATGAATGACCGTGTCAAGGGCTCCTTGAAGGTCACTCGGGCTTTCGGTGCTGGTTTTCTCAAACAGCCTAAATGGAACAATGCACTTCTAGAGGCGTTCCAAATCGACTACATAGGGACTTCCCCATACATCACTTGTTCTCCTTCTCTCCACTACCACAGATTAGGTCCCAAAGACAGGTTTTTGATATTATCCTCCGATGGATTGTATCAATACTTTACCAACGAAGAAGCTGTTTCTGAAGTTGAACTTTTCATCACATTGCAACCTGAAGGAGATCCGGCGCAGCATCTGGTTGAGGAAGTGCTGTTTCGTGCTGCAAAAAAGGCCGGAATGGACTTCCACGACCTTCTCCAAATACCACAAGGGGATCGAAGGCGGTACCATGATGATGTTTCTATCATCGTTATTTCATTAGAAGGAAGAATATGGAGATCATGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0071944 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

723

Amino Acids

80.09

Weight (kDa)

5.25

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 544 - 664 3.2e-22 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07630 AT2G28890
fragaria_vesca FvH4_3g35990 FvH4_3g35990 FvH4_3g35990
malus_domestica MD03G1094800.v1.1 MD11G1110000.v1.1
prunus_persica Prupe.6G078500_v2.0.a1
pyrus_communis pycom03g07750 pycom11g09070
rosa_chinensis RchiOBHm_Chr5g0064391
rosa_laevigata RLG00000035720
rosa_multiflora Rmu_sc0000806.1_g000039
rosa_roxburghii Rroxscaffold_1G00016610
rosa_rugosa Rorug05G0364300
rosa_samantha Rh5AG422600 Rh5BG438400 Rh5CG461100 Rh5DG451600
rosa_wichuraiana Rw5G039770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2103
AccB1I GGYRCC 1 cut(s) 2103
AccBSI CCGCTC 2 cut(s) 290, 472
AccI GTMKAC 1 cut(s) 615
AccII CGCG 3 cut(s) 447, 1173, 1494
AclWI GGATC 5 cut(s) 555, 834, 1659, 1994, 2100
AcoI YGGCCR 1 cut(s) 996
AcsI RAATTY 6 cut(s) 721, 742, 1036, 1117, 1129, 1153
AcuI CTGAAG 3 cut(s) 1365, 1983, 2013
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 3 cut(s) 932, 1169, 2105
AfiI CCNNNNNNNGG 6 cut(s) 404, 570, 1177, 1527, 1738, 1899
AflIII ACRYGT 1 cut(s) 75
AgsI TTSAA 7 cut(s) 892, 1159, 1258, 1333, 1579, 1750, 1970
AhdI GACNNNNNGTC 1 cut(s) 775
AjiI CACGTC 2 cut(s) 76, 838
AleI CACNNNNGTG 1 cut(s) 938
AloI GAACNNNNNNTCC 4 cut(s) 378, 407, 410, 439
AluBI AGCT 8 cut(s) 306, 389, 528, 952, 1243, 1304, 1642, 1955
AluI AGCT 8 cut(s) 306, 389, 528, 952, 1243, 1304, 1642, 1955
Alw21I GWGCWC 2 cut(s) 308, 837
Alw26I GTCTC 2 cut(s) 548, 1602
Alw44I GTGCAC 1 cut(s) 833
AlwI GGATC 5 cut(s) 555, 834, 1659, 1994, 2100
AlwNI CAGNNNCTG 1 cut(s) 2014
Ama87I CYCGRG 6 cut(s) 113, 343, 398, 467, 484, 1758
AoxI GGCC 3 cut(s) 895, 996, 2049
ApaLI GTGCAC 1 cut(s) 833
ApeKI GCWGC 3 cut(s) 735, 2008, 2039
ApoI RAATTY 6 cut(s) 721, 742, 1036, 1117, 1129, 1153
AseI ATTAAT 2 cut(s) 708, 1563
Asp700I GAANNNNTTC 1 cut(s) 11
Asp718I GGTACC 1 cut(s) 2103
AspLEI GCGC 5 cut(s) 224, 447, 808, 1718, 2008
AspS9I GGNCC 8 cut(s) 94, 365, 407, 434, 461, 488, 896, 1889
AsuC2I CCSGG 6 cut(s) 399, 400, 523, 565, 620, 899
AsuHPI GGTGA 4 cut(s) 28, 679, 1064, 1469
AsuII TTCGAA 2 cut(s) 1034, 1127
AvaI CYCGRG 6 cut(s) 113, 343, 398, 467, 484, 1758
AvaII GGWCC 7 cut(s) 94, 365, 407, 434, 461, 488, 1889
BaeGI GKGCMC 2 cut(s) 120, 837
BanI GGYRCC 1 cut(s) 2103
BanII GRGCYC 3 cut(s) 308, 1286, 1745
BarI GAAGNNNNNNTAC 2 cut(s) 599, 631
BbsI GAAGAC 2 cut(s) 614, 774
Bbv12I GWGCWC 2 cut(s) 308, 837
BbvI GCAGC 3 cut(s) 747, 2020, 2026
BccI CCATC 7 cut(s) 97, 347, 487, 641, 1043, 1417, 1916
BceAI ACGGC 7 cut(s) 25, 42, 79, 608, 951, 1194, 1615
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BclI TGATCA 1 cut(s) 354
BcnI CCSGG 6 cut(s) 399, 400, 523, 565, 620, 899
BcoDI GTCTC 2 cut(s) 548, 1602
BfaI CTAG 1 cut(s) 1811
BfoI RGCGCY 1 cut(s) 225
BglI GCCNNNNNGGC 1 cut(s) 63
BisI GCNGC 9 cut(s) 55, 291, 294, 691, 736, 999, 1174, 2009, 2040
BlsI GCNGC 9 cut(s) 56, 292, 295, 692, 737, 1000, 1175, 2010, 2041
Bme1390I CCNGG 6 cut(s) 399, 400, 523, 565, 620, 899
Bme18I GGWCC 7 cut(s) 94, 365, 407, 434, 461, 488, 1889
BmeRI GACNNNNNGTC 1 cut(s) 775
BmeT110I CYCGRG 6 cut(s) 113, 343, 398, 467, 484, 1758
BmgBI CACGTC 2 cut(s) 76, 838
BmgT120I GGNCC 8 cut(s) 94, 365, 407, 434, 461, 488, 896, 1889
BmrFI CCNGG 6 cut(s) 399, 400, 523, 565, 620, 899
BmrI ACTGGG 1 cut(s) 811
BmuI ACTGGG 1 cut(s) 811
BpiI GAAGAC 2 cut(s) 614, 774
Bpu14I TTCGAA 2 cut(s) 1034, 1127
BpuEI CTTGAG 1 cut(s) 1372
BpuMI CCSGG 6 cut(s) 399, 400, 523, 565, 620, 899
Bsa29I ATCGAT 1 cut(s) 282
BsaBI GATNNNNATC 1 cut(s) 553
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 5 cut(s) 398, 521, 619, 1171, 1647
BsaWI WCCGGW 4 cut(s) 206, 907, 1069, 1298
BsaXI ACNNNNNCTCC 7 cut(s) 378, 408, 845, 875, 1846, 1876, 2146
Bsc4I CCNNNNNNNGG 6 cut(s) 404, 570, 1177, 1527, 1738, 1899
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 806
Bse3DI GCAATG 1 cut(s) 1982
Bse8I GATNNNNATC 1 cut(s) 553
BseCI ATCGAT 1 cut(s) 282
BseDI CCNNGG 5 cut(s) 398, 521, 619, 1171, 1647
BseGI GGATG 9 cut(s) 158, 339, 907, 976, 1084, 1292, 1330, 1468, 1598
BseJI GATNNNNATC 1 cut(s) 553
BseLI CCNNNNNNNGG 6 cut(s) 404, 570, 1177, 1527, 1738, 1899
BseMI GCAATG 1 cut(s) 1982
BseMII CTCAG 1 cut(s) 1520
BseNI ACTGG 1 cut(s) 806
BseRI GAGGAG 4 cut(s) 866, 1150, 1219, 1379
BseSI GKGCMC 2 cut(s) 120, 837
BseXI GCAGC 3 cut(s) 747, 2020, 2026
BseYI CCCAGC 1 cut(s) 389
Bsh1236I CGCG 3 cut(s) 447, 1173, 1494
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 3 cut(s) 897, 998, 2051
BshNI GGYRCC 1 cut(s) 2103
BshVI ATCGAT 1 cut(s) 282
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 2 cut(s) 308, 837
BsiHKCI CYCGRG 6 cut(s) 113, 343, 398, 467, 484, 1758
BslFI GGGAC 3 cut(s) 123, 1852, 1875
BslI CCNNNNNNNGG 6 cut(s) 404, 570, 1177, 1527, 1738, 1899
BsmAI GTCTC 2 cut(s) 548, 1602
BsmFI GGGAC 3 cut(s) 123, 1852, 1875
BsnI GGCC 3 cut(s) 897, 998, 2051
Bso31I GGTCTC 1 cut(s) 548
BsoBI CYCGRG 6 cut(s) 113, 343, 398, 467, 484, 1758
Bsp119I TTCGAA 2 cut(s) 1034, 1127
Bsp1286I GDGCHC 5 cut(s) 120, 308, 837, 1286, 1745
Bsp1407I TGTACA 1 cut(s) 930
Bsp143I GATC 9 cut(s) 259, 283, 354, 560, 826, 1651, 1999, 2092, 2156
BspANI GGCC 3 cut(s) 897, 998, 2051
BspCNI CTCAG 1 cut(s) 1519
BspDI ATCGAT 1 cut(s) 282
BspFNI CGCG 3 cut(s) 447, 1173, 1494
BspPI GGATC 5 cut(s) 555, 834, 1659, 1994, 2100
BspT104I TTCGAA 2 cut(s) 1034, 1127
BspT107I GGYRCC 1 cut(s) 2103
BspTNI GGTCTC 1 cut(s) 548
BsrBI CCGCTC 2 cut(s) 290, 472
BsrDI GCAATG 1 cut(s) 1982
BsrFI RCCGGY 1 cut(s) 38
BsrGI TGTACA 1 cut(s) 930
BsrI ACTGG 1 cut(s) 806
BssAI RCCGGY 1 cut(s) 38
BssECI CCNNGG 5 cut(s) 398, 521, 619, 1171, 1647
BssMI GATC 9 cut(s) 259, 283, 354, 560, 826, 1651, 1999, 2092, 2156
BssNAI GTATAC 1 cut(s) 616
BssNI GRCGYC 1 cut(s) 42
Bst1107I GTATAC 1 cut(s) 616
Bst4CI ACNGT 4 cut(s) 613, 730, 1046, 1733
Bst6I CTCTTC 1 cut(s) 1665
BstACI GRCGYC 1 cut(s) 42
BstAUI TGTACA 1 cut(s) 930
BstBI TTCGAA 2 cut(s) 1034, 1127
BstC8I GCNNGC 4 cut(s) 156, 597, 695, 1714
BstDEI CTNAG 1 cut(s) 1506
BstDSI CCRYGG 2 cut(s) 1171, 1647
BstF5I GGATG 9 cut(s) 158, 339, 907, 976, 1084, 1292, 1330, 1468, 1598
BstFNI CGCG 3 cut(s) 447, 1173, 1494
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 5 cut(s) 224, 447, 808, 1718, 2008
BstKTI GATC 9 cut(s) 262, 286, 357, 563, 829, 1654, 2002, 2095, 2159
BstMAI GTCTC 2 cut(s) 548, 1602
BstMBI GATC 9 cut(s) 259, 283, 354, 560, 826, 1651, 1999, 2092, 2156
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 9 cut(s) 39, 63, 998, 1004, 1310, 1491, 1511, 1769, 2048
BstSCI CCNGG 6 cut(s) 397, 398, 521, 563, 618, 897
BstSLI GKGCMC 2 cut(s) 120, 837
BstUI CGCG 3 cut(s) 447, 1173, 1494
BstV1I GCAGC 3 cut(s) 747, 2020, 2026
BstV2I GAAGAC 2 cut(s) 614, 774
BstX2I RGATCY 2 cut(s) 560, 1999
BstYI RGATCY 2 cut(s) 560, 1999
BstZ17I GTATAC 1 cut(s) 616
Bsu15I ATCGAT 1 cut(s) 282
BsuRI GGCC 3 cut(s) 897, 998, 2051
BsuTUI ATCGAT 1 cut(s) 282
BtgI CCRYGG 2 cut(s) 1171, 1647
BtrI CACGTC 2 cut(s) 76, 838
BtsCI GGATG 9 cut(s) 158, 339, 907, 976, 1084, 1292, 1330, 1468, 1598
BtsI GCAGTG 1 cut(s) 329
BtsIMutI CAGTG 3 cut(s) 329, 799, 1051
Cac8I GCNNGC 4 cut(s) 156, 597, 695, 1714
CaiI CAGNNNCTG 1 cut(s) 2014
CfoI GCGC 5 cut(s) 224, 447, 808, 1718, 2008
Cfr10I RCCGGY 1 cut(s) 38
Cfr13I GGNCC 8 cut(s) 94, 365, 407, 434, 461, 488, 896, 1889
Cfr42I CCGCGG 1 cut(s) 1174
Cfr9I CCCGGG 1 cut(s) 398
ClaI ATCGAT 1 cut(s) 282
CseI GACGC 1 cut(s) 31
Csp6I GTAC 3 cut(s) 931, 1168, 2104
CviAII CATG 4 cut(s) 454, 739, 2108, 2160
CviQI GTAC 3 cut(s) 931, 1168, 2104
DdeI CTNAG 1 cut(s) 1506
DpnI GATC 9 cut(s) 261, 285, 356, 562, 828, 1653, 2001, 2094, 2158
DpnII GATC 9 cut(s) 259, 283, 354, 560, 826, 1651, 1999, 2092, 2156
DraI TTTAAA 1 cut(s) 553
DriI GACNNNNNGTC 1 cut(s) 775
EaeI YGGCCR 1 cut(s) 996
Eam1104I CTCTTC 1 cut(s) 1665
Eam1105I GACNNNNNGTC 1 cut(s) 775
EarI CTCTTC 1 cut(s) 1665
EciI GGCGGA 2 cut(s) 995, 1529
Ecl136II GAGCTC 1 cut(s) 306
Eco24I GRGCYC 3 cut(s) 308, 1286, 1745
Eco31I GGTCTC 1 cut(s) 548
Eco32I GATATC 1 cut(s) 603
Eco47I GGWCC 7 cut(s) 94, 365, 407, 434, 461, 488, 1889
Eco53kI GAGCTC 1 cut(s) 306
Eco57I CTGAAG 3 cut(s) 1365, 1983, 2013
Eco88I CYCGRG 6 cut(s) 113, 343, 398, 467, 484, 1758
EcoICRI GAGCTC 1 cut(s) 306
EcoO109I RGGNCCY 3 cut(s) 407, 434, 1889
EcoRI GAATTC 2 cut(s) 1036, 1129
EcoRV GATATC 1 cut(s) 603
EcoT22I ATGCAT 1 cut(s) 1294
EcoT38I GRGCYC 3 cut(s) 308, 1286, 1745
FaeI CATG 4 cut(s) 457, 742, 2111, 2163
FalI AAGNNNNNCTT 4 cut(s) 1730, 1762, 1956, 1988
FaqI GGGAC 3 cut(s) 123, 1852, 1875
FatI CATG 4 cut(s) 453, 738, 2107, 2159
FauI CCCGC 4 cut(s) 440, 465, 588, 686
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 1 cut(s) 615
Fnu4HI GCNGC 9 cut(s) 55, 291, 294, 691, 736, 999, 1174, 2009, 2040
FokI GGATG 9 cut(s) 145, 326, 914, 983, 1091, 1279, 1337, 1475, 1605
FriOI GRGCYC 3 cut(s) 308, 1286, 1745
Fsp4HI GCNGC 9 cut(s) 55, 291, 294, 691, 736, 999, 1174, 2009, 2040
FspBI CTAG 1 cut(s) 1811
GlaI GCGC 5 cut(s) 223, 446, 807, 1717, 2007
GluI GCNGC 9 cut(s) 55, 291, 294, 691, 736, 999, 1174, 2009, 2040
GsaI CCCAGC 1 cut(s) 393
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 3 cut(s) 897, 998, 2051
HgaI GACGC 1 cut(s) 31
HhaI GCGC 5 cut(s) 224, 447, 808, 1718, 2008
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 4 cut(s) 457, 742, 2111, 2163
Hin6I GCGC 5 cut(s) 222, 445, 806, 1716, 2006
HinP1I GCGC 5 cut(s) 222, 445, 806, 1716, 2006
HphI GGTGA 4 cut(s) 28, 679, 1064, 1469
Hpy166II GTNNAC 6 cut(s) 172, 464, 616, 835, 933, 943
Hpy188III TCNNGA 8 cut(s) 16, 301, 343, 413, 558, 874, 1409, 1811
Hpy8I GTNNAC 6 cut(s) 172, 464, 616, 835, 933, 943
Hpy99I CGWCG 6 cut(s) 107, 776, 842, 845, 875, 887
HpyCH4III ACNGT 4 cut(s) 613, 730, 1046, 1733
HpyCH4IV ACGT 3 cut(s) 75, 132, 837
HpyCH4V TGCA 9 cut(s) 334, 835, 905, 1007, 1103, 1292, 1805, 1987, 2042
HpyF10VI GCNNNNNNNGC 9 cut(s) 39, 63, 998, 1004, 1310, 1491, 1511, 1769, 2048
HpyF3I CTNAG 1 cut(s) 1506
HpySE526I ACGT 3 cut(s) 75, 132, 837
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 4 cut(s) 457, 742, 2111, 2163
HspAI GCGC 5 cut(s) 222, 445, 806, 1716, 2006
KpnI GGTACC 1 cut(s) 2107
Ksp22I TGATCA 1 cut(s) 354
KspI CCGCGG 1 cut(s) 1174
Kzo9I GATC 9 cut(s) 259, 283, 354, 560, 826, 1651, 1999, 2092, 2156
LmnI GCTCC 8 cut(s) 311, 386, 525, 1028, 1240, 1289, 1301, 1748
Lsp1109I GCAGC 3 cut(s) 747, 2020, 2026
MaeI CTAG 1 cut(s) 1811
MaeII ACGT 3 cut(s) 75, 132, 837
MaeIII GTNAC 5 cut(s) 59, 503, 1052, 1595, 1753
MalI GATC 9 cut(s) 261, 285, 356, 562, 828, 1653, 2001, 2094, 2158
MbiI CCGCTC 2 cut(s) 290, 472
MboI GATC 9 cut(s) 259, 283, 354, 560, 826, 1651, 1999, 2092, 2156
MflI RGATCY 2 cut(s) 560, 1999
MhlI GDGCHC 5 cut(s) 120, 308, 837, 1286, 1745
MlyI GAGTC 4 cut(s) 105, 666, 785, 796
MmeI TCCRAC 1 cut(s) 160
Mph1103I ATGCAT 1 cut(s) 1294
MroXI GAANNNNTTC 1 cut(s) 11
MseI TTAA 4 cut(s) 552, 708, 954, 1563
MslI CAYNNNNRTG 3 cut(s) 938, 1323, 2112
MspA1I CMGCKG 2 cut(s) 1173, 1642
MspR9I CCNGG 6 cut(s) 399, 400, 523, 565, 620, 899
MvnI CGCG 3 cut(s) 447, 1173, 1494
MwoI GCNNNNNNNGC 9 cut(s) 39, 63, 998, 1004, 1310, 1491, 1511, 1769, 2048
NciI CCSGG 6 cut(s) 399, 400, 523, 565, 620, 899
NdeII GATC 9 cut(s) 259, 283, 354, 560, 826, 1651, 1999, 2092, 2156
NlaIII CATG 4 cut(s) 457, 742, 2111, 2163
NmeAIII GCCGAG 1 cut(s) 680
NmuCI GTSAC 4 cut(s) 59, 1052, 1595, 1753
NsiI ATGCAT 1 cut(s) 1294
NspV TTCGAA 2 cut(s) 1034, 1127
OliI CACNNNNGTG 1 cut(s) 938
PaeR7I CTCGAG 1 cut(s) 467
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 879
PdmI GAANNNNTTC 1 cut(s) 11
PflFI GACNNNGTC 1 cut(s) 1733
PfoI TCCNGGA 1 cut(s) 563
PkrI GCNGC 9 cut(s) 56, 292, 295, 692, 737, 1000, 1175, 2010, 2041
Ple19I CGATCG 1 cut(s) 286
PleI GAGTC 4 cut(s) 105, 665, 784, 795
PpsI GAGTC 4 cut(s) 105, 665, 784, 795
PpuMI RGGWCCY 3 cut(s) 407, 434, 1889
PshBI ATTAAT 2 cut(s) 708, 1563
Psp124BI GAGCTC 1 cut(s) 308
Psp5II RGGWCCY 3 cut(s) 407, 434, 1889
PspFI CCCAGC 1 cut(s) 389
PspPI GGNCC 8 cut(s) 94, 365, 407, 434, 461, 488, 896, 1889
PspPPI RGGWCCY 3 cut(s) 407, 434, 1889
PspXI VCTCGAGB 1 cut(s) 467
PstNI CAGNNNCTG 1 cut(s) 2014
PsuI RGATCY 2 cut(s) 560, 1999
PsyI GACNNNGTC 1 cut(s) 1733
PvuI CGATCG 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 1642
RsaI GTAC 3 cut(s) 932, 1169, 2105
RsaNI GTAC 3 cut(s) 931, 1168, 2104
RseI CAYNNNNRTG 3 cut(s) 938, 1323, 2112
SacI GAGCTC 1 cut(s) 308
SacII CCGCGG 1 cut(s) 1174
SaqAI TTAA 4 cut(s) 552, 708, 954, 1563
SatI GCNGC 9 cut(s) 55, 291, 294, 691, 736, 999, 1174, 2009, 2040
Sau3AI GATC 9 cut(s) 259, 283, 354, 560, 826, 1651, 1999, 2092, 2156
Sau96I GGNCC 8 cut(s) 94, 365, 407, 434, 461, 488, 896, 1889
SchI GAGTC 4 cut(s) 105, 666, 785, 796
ScrFI CCNGG 6 cut(s) 399, 400, 523, 565, 620, 899
SduI GDGCHC 5 cut(s) 120, 308, 837, 1286, 1745
Sfr274I CTCGAG 1 cut(s) 467
Sfr303I CCGCGG 1 cut(s) 1174
SfuI TTCGAA 2 cut(s) 1034, 1127
SgrAI CRCCGGYG 1 cut(s) 38
SgrBI CCGCGG 1 cut(s) 1174
SinI GGWCC 7 cut(s) 94, 365, 407, 434, 461, 488, 1889
SlaI CTCGAG 1 cut(s) 467
SmaI CCCGGG 1 cut(s) 400
SmiI ATTTAAAT 1 cut(s) 553
SmiMI CAYNNNNRTG 3 cut(s) 938, 1323, 2112
SmlI CTYRAG 2 cut(s) 467, 1351
SmoI CTYRAG 2 cut(s) 467, 1351
SspMI CTAG 1 cut(s) 1811
SstI GAGCTC 1 cut(s) 308
StyD4I CCNGG 6 cut(s) 397, 398, 521, 563, 618, 897
SwaI ATTTAAAT 1 cut(s) 553
TaaI ACNGT 4 cut(s) 613, 730, 1046, 1733
TaiI ACGT 3 cut(s) 78, 135, 840
TatI WGTACW 1 cut(s) 930
TauI GCSGC 6 cut(s) 57, 293, 296, 693, 1001, 1176
Tru1I TTAA 4 cut(s) 552, 708, 954, 1563
Tru9I TTAA 4 cut(s) 552, 708, 954, 1563
TscAI CASTG 3 cut(s) 336, 806, 1051
TseFI GTSAC 4 cut(s) 59, 1052, 1595, 1753
TseI GCWGC 3 cut(s) 735, 2008, 2039
Tsp45I GTSAC 4 cut(s) 59, 1052, 1595, 1753
TspDTI ATGAA 8 cut(s) 442, 755, 1466, 1490, 1710, 1739, 1966, 2124
TspGWI ACGGA 3 cut(s) 205, 900, 1664
TspMI CCCGGG 1 cut(s) 398
TspRI CASTG 3 cut(s) 336, 806, 1051
Tth111I GACNNNGTC 1 cut(s) 1733
VneI GTGCAC 1 cut(s) 833
VpaK11BI GGWCC 7 cut(s) 94, 365, 407, 434, 461, 488, 1889
VspI ATTAAT 2 cut(s) 708, 1563
XapI RAATTY 6 cut(s) 721, 742, 1036, 1117, 1129, 1153
XbaI TCTAGA 1 cut(s) 1810
XhoI CTCGAG 1 cut(s) 467
XmaI CCCGGG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 615
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 1 cut(s) 1811
Zsp2I ATGCAT 1 cut(s) 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.