RchiOBHm_Chr5g0064391

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010729
Physical Location & Seq
Reverse (-)
70311945 .. 70315208
3264 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34038

Sequence Viewer

Length: 2208 bp
ATGGGCAACAGCTTTGGAAAAGTTAGCGTCTGCTTCACCGGCGCTAAAGGAGTTCATCGTCGAACCCAAGATGAACCGCTTGTATTATCCGACACCTACGACGAGGGCCTCGGCCACTCTTTCTGCTATGTCCGACCCGACCCGACCTGGCTGGCCTCTTCTAAGGTCCACTCCGAGGAGGCCACCACCTTCAAAACCATCTCCGGAGCTTCGGTCAGCGCCAACACGTCGACGCCTCTGTCCACGGCCGTCCTCGATCTCTACTCTTACAACAGCATCGATCGGGCGGCGGCTTTCGAGTCCTCCACCTCTTTCGCCTCGATTCCTCTGCAGCCCATCCCGCGAAATCTCATCAATTCGGGTCCGATTTCGGGGAACTTCGGAGCGGGGATCCCCGGGTCGGGTCCGCTCGAGCGAGGGTTCCTGTCCGGCCCAATCGAGCGGGCGTTCATGTCCGGCCCGCTCGACCGTGGGCTCTTCTCCGGGCCCATGGAGAAGGGGTACTCGGAGCAGTTTCAGAGGAGCTTCTCCCACGGCGGCGGGTTCGCTTTCAAGCCGAGATCGGGGAAGCGGAAACTGATTCGGGTTCTTCAGAGAGCGATATCGAAGACGATATATCGTGGCCAGAACTCCATTCTCGCGCCGATTAAAAACCGCGTCGTTTCGGTGAAAGATCAGGATTGGATCGTCGGCGGCGGCGGCGGAGGACTGCAAGATAGTAAGCTTCAAAATCACAACGAGAATTTGACCGTGAGCTCTGTGAATTTCAGCAGTGAGGGGAGTTTGGAAGACGACGAGTCGTTGTCGAGTCAAAATCTTCAGTGGGCGCAAGGGAAAGCAGGGGAGGATCGAGTCCACGTCGTCGTTTCGGAGGAGGACGGCTGGGTTTTCGTCGGAATCTACGATGGATTCAACGGCCCAGATGCGCCGGATTATCTTCTATCGAATTTGTACACAGCTGTCCATAAGGAGCTGAAAGGGTTGATTTGGGACGAGAGGCTTGAATCAGCTGCGGTGGCCGCGCCTGCGACTTCCCCTGTTCACCCAGAATATGCGGATTCGGTTTCGGTTTCGATTTCGATTTCGGATAGTGATTTGGGAAGGAATCAAGTGGCAGATGCTTGTTCTCAGTTTGTTGAGGAAGAGAATTATCCTTGTGCAAGTGGGGATGTGAATTTCGATTCCAATTCCAAGAAAAGGGAGTCCAGGAACCCGAAAACAAAGTACCGGAGCTCCGGCCGGAAATGGGAGGATAATCAGAGGAGGTGGAGGTGTGAATGGGATAGAGAGAGAGTTGAGCTTGATCGGAGATTGAAGGAACAGCTTAATAGGTCCAGCGCAGATGGGTCATCGGCTACTAACCACACTGATGTTTTGAAGGCTTTGTCTCAAGCTTTGAGGAAAACAGAGGAGGCGTATTTGGAAGTTGCGGATAAAATGCTCTTGGAAAATCCGGAGTTGGCCTTGATGGGGTCTTGTGTTCTAGTGATGTTGATGAAGGGGGAGGATGTTTATGTGATGAATGTGGGTGATAGTCGGGTTGTTTTGGCCCAGAAGGCGGAACCGGATTATTGGCTTGGGAAGATTAGGCAGGATTTGGAGAGGATCAATGAGGAAACATTACATGATCTTGAGGACGGCGAAAGACAGAATGCAATTCCCAATTTGAGCGCTGTTCAGCTGACGATGGATCACAGTACTAGCGAGCAAGAGGAAGTTCATAGAATCCGAAATGAACACCCAGAGGATGCTTGTGCTGTCATGAATGATCGTGTTAAAGGTTCATTGAAGGTCACTAGAGCTTTTGGTGCCGGCTTTCTCAAACAGCCTAAATGGAACAATGCACTTCTAGAGGCATTCCAAATTGATTATATAGGGACTTCCCCCTACATCACTTGCTTCCCATATCTCCACCATCACAGATTAGGCCCTAAAGACAGGTTCTTGATATTATCCTCGGATGGGTTGTATCAGTACTTCACGAACGAAGAAGCTGTTTCTGAAGTTGAACTTTTCATCACATTACAACCTGAAGGAGATCCGGCACAGCATCTTGTAGAGGAAGTATTGTTTCGTGCTGCCAAAAAAGCTGGTATGGACTTCCACGAATTGCTTCAAATACCACAAGGGGATCGGAGGCGGTACCATGATGATGTTTCCATCATCGTTATTTCTTTAGAGGGAAGAATATGGAGATCATGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0071944 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

735

Amino Acids

81.71

Weight (kDa)

5.43

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 439 - 677 1e-29 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07630 AT2G28890
fragaria_vesca FvH4_3g35990 FvH4_3g35990 FvH4_3g35990
malus_domestica MD03G1094800.v1.1 MD11G1110000.v1.1
prunus_persica Prupe.6G078500_v2.0.a1
pyrus_communis pycom03g07750 pycom11g09070
rosa_chinensis RchiOBHm_Chr5g0064391
rosa_laevigata RLG00000035720
rosa_multiflora Rmu_sc0000806.1_g000039
rosa_roxburghii Rroxscaffold_1G00016610
rosa_rugosa Rorug05G0364300
rosa_samantha Rh5AG422600 Rh5BG438400 Rh5CG461100 Rh5DG451600
rosa_wichuraiana Rw5G039770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 238
Acc65I GGTACC 1 cut(s) 2142
AccB1I GGYRCC 2 cut(s) 1808, 2142
AccBSI CCGCTC 4 cut(s) 386, 409, 442, 463
AccI GTMKAC 1 cut(s) 230
AccII CGCG 4 cut(s) 343, 641, 657, 1022
AccIII TCCGGA 2 cut(s) 203, 1453
AclWI GGATC 8 cut(s) 385, 398, 692, 855, 1613, 1698, 2033, 2139
AcoI YGGCCR 5 cut(s) 112, 246, 622, 1017, 1237
AcsI RAATTY 4 cut(s) 742, 763, 946, 1174
AcuI CTGAAG 4 cut(s) 575, 803, 2022, 2052
AcyI GRCGYC 1 cut(s) 233
AfaI GTAC 6 cut(s) 503, 953, 1226, 1699, 1976, 2144
AfeI AGCGCT 1 cut(s) 1672
AflIII ACRYGT 1 cut(s) 225
AhdI GACNNNNNGTC 1 cut(s) 796
AjiI CACGTC 2 cut(s) 228, 859
AjnI CCWGG 2 cut(s) 146, 1205
Alw21I GWGCWC 2 cut(s) 758, 1235
Alw26I GTCTC 1 cut(s) 1392
AlwI GGATC 8 cut(s) 385, 398, 692, 855, 1613, 1698, 2033, 2139
Ama87I CYCGRG 2 cut(s) 395, 410
Aor13HI TCCGGA 2 cut(s) 203, 1453
Aor51HI AGCGCT 1 cut(s) 1672
ApaI GGGCCC 1 cut(s) 489
ApeKI GCWGC 3 cut(s) 331, 1010, 2078
ApoI RAATTY 4 cut(s) 742, 763, 946, 1174
Asp700I GAANNNNTTC 1 cut(s) 2112
Asp718I GGTACC 1 cut(s) 2142
AspLEI GCGC 8 cut(s) 44, 221, 643, 829, 928, 1024, 1340, 1673
AsuC2I CCSGG 3 cut(s) 396, 397, 484
AsuHPI GGTGA 4 cut(s) 28, 679, 1034, 1541
AvaI CYCGRG 2 cut(s) 395, 410
AvaII GGWCC 4 cut(s) 166, 362, 404, 1332
BaeGI GKGCMC 1 cut(s) 489
BalI TGGCCA 1 cut(s) 624
BamHI GGATCC 1 cut(s) 390
BanI GGYRCC 2 cut(s) 1808, 2142
BanII GRGCYC 4 cut(s) 477, 489, 758, 1235
BarI GAAGNNNNNNTAC 2 cut(s) 1961, 1993
BbsI GAAGAC 2 cut(s) 614, 795
Bbv12I GWGCWC 2 cut(s) 758, 1235
BbvI GCAGC 3 cut(s) 343, 997, 2065
BccI CCATC 9 cut(s) 206, 344, 899, 1337, 1462, 1681, 1923, 1955, 2168
BceAI ACGGC 6 cut(s) 233, 261, 550, 895, 931, 1654
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 2 cut(s) 148, 1207
BcnI CCSGG 3 cut(s) 396, 397, 484
BcoDI GTCTC 1 cut(s) 1392
BfaI CTAG 4 cut(s) 1484, 1701, 1797, 1850
BfmI CTRYAG 1 cut(s) 329
BfoI RGCGCY 3 cut(s) 45, 222, 1674
BglI GCCNNNNNGGC 1 cut(s) 1556
BmcAI AGTACT 2 cut(s) 1699, 1976
Bme1390I CCNGG 5 cut(s) 148, 396, 397, 484, 1207
Bme18I GGWCC 4 cut(s) 166, 362, 404, 1332
BmeRI GACNNNNNGTC 1 cut(s) 796
BmeT110I CYCGRG 2 cut(s) 395, 410
BmgBI CACGTC 2 cut(s) 228, 859
BmiI GGNNCC 9 cut(s) 363, 392, 405, 422, 487, 1211, 1563, 1810, 2144
BmrFI CCNGG 5 cut(s) 148, 396, 397, 484, 1207
BmsI GCATC 5 cut(s) 285, 913, 1108, 1738, 2059
BpiI GAAGAC 2 cut(s) 614, 795
BpuEI CTTGAG 2 cut(s) 1374, 1652
BpuMI CCSGG 3 cut(s) 396, 397, 484
Bsa29I ATCGAT 1 cut(s) 279
BsaHI GRCGYC 1 cut(s) 233
BsaJI CCNNGG 9 cut(s) 109, 174, 243, 394, 395, 469, 489, 532, 1956
BsaWI WCCGGW 4 cut(s) 203, 1227, 1453, 1564
BsaXI ACNNNNNCTCC 7 cut(s) 198, 228, 485, 515, 1217, 1247, 2185
Bse118I RCCGGY 2 cut(s) 38, 1811
BseAI TCCGGA 2 cut(s) 203, 1453
BseBI CCWGG 2 cut(s) 148, 1207
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 9 cut(s) 109, 174, 243, 394, 395, 469, 489, 532, 1956
BseGI GGATG 5 cut(s) 336, 1174, 1513, 1753, 1966
BseMII CTCAG 1 cut(s) 1142
BseRI GAGGAG 5 cut(s) 191, 535, 887, 1276, 1424
BseSI GKGCMC 1 cut(s) 489
BseX3I CGGCCG 2 cut(s) 246, 1237
BseXI GCAGC 3 cut(s) 343, 997, 2065
BseYI CCCAGC 1 cut(s) 882
Bsh1236I CGCG 4 cut(s) 343, 641, 657, 1022
Bsh1285I CGRYCG 4 cut(s) 249, 283, 469, 1240
BshNI GGYRCC 2 cut(s) 1808, 2142
BshVI ATCGAT 1 cut(s) 279
BsiEI CGRYCG 4 cut(s) 249, 283, 469, 1240
BsiHKAI GWGCWC 2 cut(s) 758, 1235
BsiHKCI CYCGRG 2 cut(s) 395, 410
BslFI GGGAC 2 cut(s) 1004, 1891
BsmAI GTCTC 1 cut(s) 1392
BsmFI GGGAC 2 cut(s) 1004, 1891
BsmI GAATGC 2 cut(s) 1657, 1856
BsoBI CYCGRG 2 cut(s) 395, 410
Bsp120I GGGCCC 1 cut(s) 485
Bsp1286I GDGCHC 4 cut(s) 477, 489, 758, 1235
Bsp13I TCCGGA 2 cut(s) 203, 1453
Bsp1407I TGTACA 1 cut(s) 951
Bsp19I CCATGG 1 cut(s) 489
BspCNI CTCAG 1 cut(s) 1141
BspDI ATCGAT 1 cut(s) 279
BspEI TCCGGA 2 cut(s) 203, 1453
BspFNI CGCG 4 cut(s) 343, 641, 657, 1022
BspHI TCATGA 1 cut(s) 1761
BspLI GGNNCC 9 cut(s) 363, 392, 405, 422, 487, 1211, 1563, 1810, 2144
BspMAI CTGCAG 1 cut(s) 333
BspPI GGATC 8 cut(s) 385, 398, 692, 855, 1613, 1698, 2033, 2139
BspQI GCTCTTC 1 cut(s) 482
BspT107I GGYRCC 2 cut(s) 1808, 2142
BsrBI CCGCTC 4 cut(s) 386, 409, 442, 463
BsrFI RCCGGY 2 cut(s) 38, 1811
BsrGI TGTACA 1 cut(s) 951
BssAI RCCGGY 2 cut(s) 38, 1811
BssECI CCNNGG 9 cut(s) 109, 174, 243, 394, 395, 469, 489, 532, 1956
BssNI GRCGYC 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 489
Bst2UI CCWGG 2 cut(s) 148, 1207
Bst4CI ACNGT 3 cut(s) 470, 751, 1697
Bst6I CTCTTC 3 cut(s) 163, 482, 1137
BstACI GRCGYC 1 cut(s) 233
BstAUI TGTACA 1 cut(s) 951
BstC8I GCNNGC 6 cut(s) 153, 444, 461, 1026, 1706, 1813
BstDEI CTNAG 2 cut(s) 162, 1128
BstDSI CCRYGG 4 cut(s) 243, 469, 489, 532
BstF5I GGATG 5 cut(s) 336, 1174, 1513, 1753, 1966
BstFNI CGCG 4 cut(s) 343, 641, 657, 1022
BstH2I RGCGCY 3 cut(s) 45, 222, 1674
BstHHI GCGC 8 cut(s) 44, 221, 643, 829, 928, 1024, 1340, 1673
BstMAI GTCTC 1 cut(s) 1392
BstMCI CGRYCG 4 cut(s) 249, 283, 469, 1240
BstMWI GCNNNNNNNGC 9 cut(s) 39, 340, 699, 1016, 1019, 1025, 1556, 1808, 2087
BstNI CCWGG 2 cut(s) 148, 1207
BstSCI CCNGG 5 cut(s) 146, 394, 395, 482, 1205
BstSFI CTRYAG 1 cut(s) 329
BstSLI GKGCMC 1 cut(s) 489
BstUI CGCG 4 cut(s) 343, 641, 657, 1022
BstV1I GCAGC 3 cut(s) 343, 997, 2065
BstV2I GAAGAC 2 cut(s) 614, 795
BstX2I RGATCY 2 cut(s) 390, 2038
BstYI RGATCY 2 cut(s) 390, 2038
BstZI CGGCCG 2 cut(s) 246, 1237
Bsu15I ATCGAT 1 cut(s) 279
BsuTUI ATCGAT 1 cut(s) 279
BtgI CCRYGG 4 cut(s) 243, 469, 489, 532
BtrI CACGTC 2 cut(s) 228, 859
BtsCI GGATG 5 cut(s) 336, 1174, 1513, 1753, 1966
BtsI GCAGTG 1 cut(s) 778
BtsIMutI CAGTG 3 cut(s) 778, 827, 1365
Cac8I GCNNGC 6 cut(s) 153, 444, 461, 1026, 1706, 1813
CciI TCATGA 1 cut(s) 1761
CfoI GCGC 8 cut(s) 44, 221, 643, 829, 928, 1024, 1340, 1673
Cfr10I RCCGGY 2 cut(s) 38, 1811
Cfr9I CCCGGG 1 cut(s) 395
ClaI ATCGAT 1 cut(s) 279
CseI GACGC 3 cut(s) 16, 241, 646
Csp6I GTAC 6 cut(s) 502, 952, 1225, 1698, 1975, 2143
CviAII CATG 6 cut(s) 451, 490, 1625, 1762, 2147, 2199
CviQI GTAC 6 cut(s) 502, 952, 1225, 1698, 1975, 2143
DdeI CTNAG 2 cut(s) 162, 1128
DrdI GACNNNNNNGTC 1 cut(s) 238
DriI GACNNNNNGTC 1 cut(s) 796
DseDI GACNNNNNNGTC 1 cut(s) 238
EaeI YGGCCR 5 cut(s) 112, 246, 622, 1017, 1237
EagI CGGCCG 2 cut(s) 246, 1237
Eam1104I CTCTTC 3 cut(s) 163, 482, 1137
Eam1105I GACNNNNNGTC 1 cut(s) 796
EarI CTCTTC 3 cut(s) 163, 482, 1137
EciI GGCGGA 2 cut(s) 717, 1574
Ecl136II GAGCTC 2 cut(s) 756, 1233
EclXI CGGCCG 2 cut(s) 246, 1237
Eco130I CCWWGG 1 cut(s) 489
Eco24I GRGCYC 4 cut(s) 477, 489, 758, 1235
Eco32I GATATC 1 cut(s) 603
Eco47I GGWCC 4 cut(s) 166, 362, 404, 1332
Eco47III AGCGCT 1 cut(s) 1672
Eco52I CGGCCG 2 cut(s) 246, 1237
Eco53kI GAGCTC 2 cut(s) 756, 1233
Eco57I CTGAAG 4 cut(s) 575, 803, 2022, 2052
Eco88I CYCGRG 2 cut(s) 395, 410
EcoICRI GAGCTC 2 cut(s) 756, 1233
EcoO109I RGGNCCY 2 cut(s) 106, 1928
EcoRII CCWGG 2 cut(s) 146, 1205
EcoRV GATATC 1 cut(s) 603
EcoT14I CCWWGG 1 cut(s) 489
EcoT38I GRGCYC 4 cut(s) 477, 489, 758, 1235
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 6 cut(s) 454, 493, 1628, 1765, 2150, 2202
FalI AAGNNNNNCTT 2 cut(s) 1995, 2027
FaqI GGGAC 2 cut(s) 1004, 1891
FatI CATG 6 cut(s) 450, 489, 1624, 1761, 2146, 2198
FauI CCCGC 5 cut(s) 348, 379, 435, 468, 533
FblI GTMKAC 1 cut(s) 230
FokI GGATG 5 cut(s) 323, 1181, 1520, 1760, 1973
FriOI GRGCYC 4 cut(s) 477, 489, 758, 1235
FspBI CTAG 4 cut(s) 1484, 1701, 1797, 1850
GlaI GCGC 8 cut(s) 43, 220, 642, 828, 927, 1023, 1339, 1672
GsaI CCCAGC 1 cut(s) 886
HaeII RGCGCY 3 cut(s) 45, 222, 1674
HgaI GACGC 3 cut(s) 16, 241, 646
HhaI GCGC 8 cut(s) 44, 221, 643, 829, 928, 1024, 1340, 1673
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 6 cut(s) 454, 493, 1628, 1765, 2150, 2202
Hin6I GCGC 8 cut(s) 42, 219, 641, 827, 926, 1022, 1338, 1671
HinP1I GCGC 8 cut(s) 42, 219, 641, 827, 926, 1022, 1338, 1671
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HindIII AAGCTT 2 cut(s) 722, 1392
HphI GGTGA 4 cut(s) 28, 679, 1034, 1541
Hpy166II GTNNAC 6 cut(s) 169, 231, 243, 856, 954, 1042
Hpy188III TCNNGA 8 cut(s) 204, 677, 1454, 1631, 1762, 1850, 1945, 1981
Hpy8I GTNNAC 6 cut(s) 169, 231, 243, 856, 954, 1042
HpyAV CCTTC 9 cut(s) 199, 490, 1095, 1309, 1372, 1492, 1549, 1783, 2027
HpyCH4III ACNGT 3 cut(s) 470, 751, 1697
HpyCH4IV ACGT 2 cut(s) 227, 858
HpyCH4V TGCA 5 cut(s) 331, 712, 1160, 1655, 1844
HpyF10VI GCNNNNNNNGC 9 cut(s) 39, 340, 699, 1016, 1019, 1025, 1556, 1808, 2087
HpyF3I CTNAG 2 cut(s) 162, 1128
HpySE526I ACGT 2 cut(s) 227, 858
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 6 cut(s) 454, 493, 1628, 1765, 2150, 2202
HspAI GCGC 8 cut(s) 42, 219, 641, 827, 926, 1022, 1338, 1671
Kpn2I TCCGGA 2 cut(s) 203, 1453
KpnI GGTACC 1 cut(s) 2146
KroI GCCGGC 1 cut(s) 1811
KroNI GCCGGC 1 cut(s) 1813
LguI GCTCTTC 1 cut(s) 482
LmnI GCTCC 7 cut(s) 206, 383, 508, 522, 970, 1230, 1238
Lsp1109I GCAGC 3 cut(s) 343, 997, 2065
LweI GCATC 5 cut(s) 285, 913, 1108, 1738, 2059
MaeI CTAG 4 cut(s) 1484, 1701, 1797, 1850
MaeII ACGT 2 cut(s) 227, 858
MaeIII GTNAC 1 cut(s) 1792
MbiI CCGCTC 4 cut(s) 386, 409, 442, 463
MflI RGATCY 2 cut(s) 390, 2038
MhlI GDGCHC 4 cut(s) 477, 489, 758, 1235
MlsI TGGCCA 1 cut(s) 624
MluNI TGGCCA 1 cut(s) 624
MlyI GAGTC 5 cut(s) 308, 806, 817, 861, 1211
MmeI TCCRAC 3 cut(s) 114, 157, 874
Mox20I TGGCCA 1 cut(s) 624
MroI TCCGGA 2 cut(s) 203, 1453
MroNI GCCGGC 1 cut(s) 1811
MroXI GAANNNNTTC 1 cut(s) 2112
MscI TGGCCA 1 cut(s) 624
MseI TTAA 3 cut(s) 648, 1326, 1776
MslI CAYNNNNRTG 2 cut(s) 1368, 2151
Msp20I TGGCCA 1 cut(s) 624
MspA1I CMGCKG 3 cut(s) 959, 1010, 1681
MspR9I CCNGG 5 cut(s) 148, 396, 397, 484, 1207
Mva1269I GAATGC 2 cut(s) 1657, 1856
MvaI CCWGG 2 cut(s) 148, 1207
MvnI CGCG 4 cut(s) 343, 641, 657, 1022
MwoI GCNNNNNNNGC 9 cut(s) 39, 340, 699, 1016, 1019, 1025, 1556, 1808, 2087
NaeI GCCGGC 1 cut(s) 1813
NciI CCSGG 3 cut(s) 396, 397, 484
NcoI CCATGG 1 cut(s) 489
NgoMIV GCCGGC 1 cut(s) 1811
NlaIII CATG 6 cut(s) 454, 493, 1628, 1765, 2150, 2202
NlaIV GGNNCC 9 cut(s) 363, 392, 405, 422, 487, 1211, 1563, 1810, 2144
NmeAIII GCCGAG 2 cut(s) 90, 582
NmuCI GTSAC 1 cut(s) 1792
PaeR7I CTCGAG 1 cut(s) 410
PagI TCATGA 1 cut(s) 1761
PciSI GCTCTTC 1 cut(s) 482
PcsI WCGNNNNNNNCGW 1 cut(s) 900
PctI GAATGC 2 cut(s) 1657, 1856
PdiI GCCGGC 1 cut(s) 1813
PdmI GAANNNNTTC 1 cut(s) 2112
PfeI GAWTC 9 cut(s) 322, 580, 897, 909, 1004, 1058, 1105, 1181, 1725
PfoI TCCNGGA 1 cut(s) 1205
Ple19I CGATCG 1 cut(s) 283
PleI GAGTC 5 cut(s) 307, 805, 816, 860, 1210
PpsI GAGTC 5 cut(s) 307, 805, 816, 860, 1210
Psp124BI GAGCTC 2 cut(s) 758, 1235
Psp6I CCWGG 2 cut(s) 146, 1205
PspFI CCCAGC 1 cut(s) 882
PspGI CCWGG 2 cut(s) 146, 1205
PspN4I GGNNCC 9 cut(s) 363, 392, 405, 422, 487, 1211, 1563, 1810, 2144
PspOMI GGGCCC 1 cut(s) 485
PspXI VCTCGAGB 1 cut(s) 410
PstI CTGCAG 1 cut(s) 333
PsuI RGATCY 2 cut(s) 390, 2038
PvuI CGATCG 1 cut(s) 283
PvuII CAGCTG 3 cut(s) 959, 1010, 1681
RsaI GTAC 6 cut(s) 503, 953, 1226, 1699, 1976, 2144
RsaNI GTAC 6 cut(s) 502, 952, 1225, 1698, 1975, 2143
RseI CAYNNNNRTG 2 cut(s) 1368, 2151
SacI GAGCTC 2 cut(s) 758, 1235
SalI GTCGAC 1 cut(s) 229
SapI GCTCTTC 1 cut(s) 482
SaqAI TTAA 3 cut(s) 648, 1326, 1776
ScaI AGTACT 2 cut(s) 1699, 1976
SchI GAGTC 5 cut(s) 308, 806, 817, 861, 1211
ScrFI CCNGG 5 cut(s) 148, 396, 397, 484, 1207
SduI GDGCHC 4 cut(s) 477, 489, 758, 1235
SfaNI GCATC 5 cut(s) 285, 913, 1108, 1738, 2059
SfcI CTRYAG 1 cut(s) 329
Sfr274I CTCGAG 1 cut(s) 410
SgrAI CRCCGGYG 1 cut(s) 38
SgrDI CGTCGACG 1 cut(s) 229
SinI GGWCC 4 cut(s) 166, 362, 404, 1332
SlaI CTCGAG 1 cut(s) 410
SmaI CCCGGG 1 cut(s) 397
SmiMI CAYNNNNRTG 2 cut(s) 1368, 2151
SmlI CTYRAG 3 cut(s) 410, 1389, 1631
SmoI CTYRAG 3 cut(s) 410, 1389, 1631
SspMI CTAG 4 cut(s) 1484, 1701, 1797, 1850
SstI GAGCTC 2 cut(s) 758, 1235
StyD4I CCNGG 5 cut(s) 146, 394, 395, 482, 1205
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 3 cut(s) 470, 751, 1697
TaiI ACGT 2 cut(s) 230, 861
TaqII GACCGA 1 cut(s) 202
TatI WGTACW 3 cut(s) 951, 1697, 1974
TauI GCSGC 7 cut(s) 290, 293, 540, 696, 699, 702, 1022
TfiI GAWTC 9 cut(s) 322, 580, 897, 909, 1004, 1058, 1105, 1181, 1725
Tru1I TTAA 3 cut(s) 648, 1326, 1776
Tru9I TTAA 3 cut(s) 648, 1326, 1776
TscAI CASTG 3 cut(s) 778, 827, 1372
TseFI GTSAC 1 cut(s) 1792
TseI GCWGC 3 cut(s) 331, 1010, 2078
Tsp45I GTSAC 1 cut(s) 1792
TspMI CCCGGG 1 cut(s) 395
TspRI CASTG 3 cut(s) 778, 827, 1372
VpaK11BI GGWCC 4 cut(s) 166, 362, 404, 1332
XapI RAATTY 4 cut(s) 742, 763, 946, 1174
XbaI TCTAGA 1 cut(s) 1849
XhoI CTCGAG 1 cut(s) 410
XmaI CCCGGG 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 230
XmnI GAANNNNTTC 1 cut(s) 2112
XspI CTAG 4 cut(s) 1484, 1701, 1797, 1850
ZrmI AGTACT 2 cut(s) 1699, 1976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.