MD11G1110000.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010729
Physical Location & Seq
Forward (+)
9719909 .. 9723186
3278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1110000.v1.1.491

Sequence Viewer

Length: 2163 bp
ATGGGGAACGGCTTCGGGAAAGTGAGCGGGTGCTTCACCGGCGTCAAGGAAGGCCGCCGTCACGGCAGACACGACGAGTCAGTTTTAGAATCGAACCAATACGACGAGGGACTCGGCCACTCCTTCTGCTATGTCCGACCCGACCCAGCTTGGCTCGCATCCTCCAAAGTCCACTCCGAAGAAGCAACCACCTTCAGAACCATCTCCGGCGCTTCCGTCAGCGCCAATACCTCCACCCCTCTATCCACCTCCCTAATCGATCTCTACTCCTACAACAGCATCGATCGTGCTGCCGCTTTCGAGAGCTCCACCTCTTTCGCTTCGATTCCACTGCAGCCCATCCCGAGAAATCTGATCAACTCGGGTCCGATTTCGGGCGCTGGGATTCCCGGGTCGGGTCCTTTGGAAAGAGGGTTCCTATCGGGTCCGATCGAGCGCGGGTTCATGTCCGGTCCACTCGATCGGGGCATCTTCTCGGGTCCTATGGAAAAGGGTTACTCCGAGCAGTTGCCTCGGAGCTTCTCGATCGGCGGTCTCGGGTTTAAATCGAGATCCGGGAAGGGCAAGCTTATTCAGGTATTACGGCGAGCGATATCGAAGACGATGTATCGGGGTCAGAACGCCATCGCTATGCCGATAAAAAGCCGCGTCGTTTCGGTCAAGGAGGATTGGGTTGTCGGCGGCGGACAGGAGAAGATTAATCACAACGAGAATTTGACCGTTAGCAGCGTGAATTTCAGTAGCGAGGGGAGTTTGGAAGACGACGAGTCGTTGGCGAGTCAGAATCTTCAGTGGGCGCAGGGAAAGGCAGGTGAAGATCGGGTGCACGTAGTCGTTTCGGAGGAGGACGGGTGGGTTTTCGTCGGGATTTACGACGGATTTAACGGCCCCGATGCGCCGGATTACTTGATGTCTAACTTGTACACGGCTGTGAACAAGGAGCTTAAAGGGTTGTTGTGGGATGAGAAGGCGGAATCGGGTGCCATTGCCGCCCCTGCAACCTCCCCTGTTCGCTCCGAAGATTCGAATTCGGAGAGTGATGGTGATTTGGGAAGGAATCCGGTGGTGGATGAGCAAGAGAATTATCCTTGTGCAAGTGGGGAGGGGAATTTAGATTCGAATTCGAGGAGAAAAGAAGGGAGGAATTTGAAAACAAAGTACCGCGGCACGGGGAGGAAATGGGAGGATAGTCAGAGGAGGTGGAGGTGTGAGTGGGATAGAGAGAGAGTAGAGCTTGATCGGAGGTTAAAGGAACAGCTCAGTAAGTTTTTGGGTTCCGATGGATCGTCCACCGGAGCTTCAAGTGCGAATCACTCGGATGTTTTGAAAGCTCTGTCTGAAGCCTTGAGAAAAACAGAGGAGGCCTATTTGGAAGTTGCTGATAGGATGCTTTTGGAAAATCCTGAATTGGCTCTGATGGGTTCTTGTGTTCTTGTAATGCTGATGAAGGGTGAGGATGTTTATGTGATGAATGTGGGCGATAGTCGGGTGGTTTTGGCGCACAAGGCGGAACCCGATTATTGGTTAGGGAAGATTAGGCAGGATTTGGAGAGGATTAATGAGGAAACTTTGAATGATATGGAAGGATGTGATGGCGAGAGAGGCAATGCGATTCCCAATTTGACTGCTTTTCAGCTGACCACGGATCACAGCACAAGTGAAGAAGAGGAAGTTCAGAGAATCCGAAATGAACACCCCGAGGATGCTTGCGCTGTGATGAATGACCGTGTCAAGGGCTCGTTGAAGGTCACTCGGGCATTCGGTGCAGGTTTTCTCAAACAGCCTAAATGGAACAATGCACTTCTAGAGGCGTTCCAAATCGACTACATAGGGACTTCCCCGTACATCACTTGTTCTCCGTCTCTCCACCACCACAGATTAGGTCCCAAAGACAGGTTTTTGATATTATCCTCTGATGGATTGTATCAATACTTTACCAACGAAGAAGCTGTTTCCGAAGTTGAACTTTTCATCACATTGCAACCTGAAGGAGATCCGGCGCAGCATCTGGTTGAGGAAGTGCTGTTTCGTGCTGCAAAAAAGGCCGGAATGGACTTCCACGACCTTCTCCAAATACCACAAGGGGATCGAAGGCGGTACCATGATGATGTTTCTGTCATTGTTATCTCCCTAGAAGGAAGAATATGGAGATCATGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0071944 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

721

Amino Acids

79.57

Weight (kDa)

5.27

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 542 - 662 1.1e-22 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07630 AT2G28890
fragaria_vesca FvH4_3g35990 FvH4_3g35990 FvH4_3g35990
malus_domestica MD03G1094800.v1.1 MD11G1110000.v1.1
prunus_persica Prupe.6G078500_v2.0.a1
pyrus_communis pycom03g07750 pycom11g09070
rosa_chinensis RchiOBHm_Chr5g0064391
rosa_laevigata RLG00000035720
rosa_multiflora Rmu_sc0000806.1_g000039
rosa_roxburghii Rroxscaffold_1G00016610
rosa_rugosa Rorug05G0364300
rosa_samantha Rh5AG422600 Rh5BG438400 Rh5CG461100 Rh5DG451600
rosa_wichuraiana Rw5G039770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 800
Acc36I ACCTGC 2 cut(s) 800, 1757
Acc65I GGTACC 1 cut(s) 2097
AccB1I GGYRCC 2 cut(s) 980, 2097
AccBSI CCGCTC 1 cut(s) 27
AccII CGCG 3 cut(s) 438, 648, 1164
AclWI GGATC 5 cut(s) 546, 1291, 1653, 1988, 2094
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 6 cut(s) 712, 733, 1027, 1108, 1120, 1144
AcuI CTGAAG 4 cut(s) 178, 773, 1359, 2007
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 4 cut(s) 923, 1160, 1844, 2099
AfiI CCNNNNNNNGG 6 cut(s) 374, 395, 1168, 1521, 1732, 1893
AgsI TTSAA 6 cut(s) 1150, 1302, 1327, 1573, 1744, 1964
AhdI GACNNNNNGTC 1 cut(s) 766
AleI CACNNNNGTG 1 cut(s) 929
AloI GAACNNNNNNTCC 2 cut(s) 398, 430
Alw21I GWGCWC 2 cut(s) 308, 828
Alw26I GTCTC 2 cut(s) 539, 1866
Alw44I GTGCAC 1 cut(s) 824
AlwI GGATC 5 cut(s) 546, 1291, 1653, 1988, 2094
AlwNI CAGNNNCTG 1 cut(s) 2008
Ama87I CYCGRG 7 cut(s) 343, 361, 389, 475, 536, 1697, 1752
AoxI GGCC 5 cut(s) 52, 115, 886, 1362, 2043
ApaLI GTGCAC 1 cut(s) 824
ApeKI GCWGC 5 cut(s) 290, 334, 726, 2002, 2033
ApoI RAATTY 6 cut(s) 712, 733, 1027, 1108, 1120, 1144
AseI ATTAAT 2 cut(s) 699, 1557
Asp700I GAANNNNTTC 1 cut(s) 11
Asp718I GGTACC 1 cut(s) 2097
AspLEI GCGC 9 cut(s) 212, 224, 380, 438, 799, 898, 1501, 1712, 2002
AspS9I GGNCC 7 cut(s) 365, 398, 425, 452, 479, 887, 1883
AsuC2I CCSGG 3 cut(s) 390, 391, 556
AsuHPI GGTGA 4 cut(s) 28, 824, 1055, 1463
AsuII TTCGAA 2 cut(s) 1025, 1118
AvaI CYCGRG 7 cut(s) 343, 361, 389, 475, 536, 1697, 1752
AvaII GGWCC 6 cut(s) 365, 398, 425, 452, 479, 1883
BaeGI GKGCMC 1 cut(s) 828
BanI GGYRCC 2 cut(s) 980, 2097
BanII GRGCYC 2 cut(s) 308, 1739
BbsI GAAGAC 2 cut(s) 605, 765
Bbv12I GWGCWC 2 cut(s) 308, 828
BbvI GCAGC 5 cut(s) 277, 346, 738, 2014, 2020
BccI CCATC 8 cut(s) 209, 347, 632, 1034, 1274, 1411, 1586, 1910
BceAI ACGGC 6 cut(s) 25, 42, 79, 599, 901, 942
BcgI CGANNNNNNTGC 6 cut(s) 272, 306, 313, 347, 494, 528
BclI TGATCA 1 cut(s) 354
BcnI CCSGG 3 cut(s) 390, 391, 556
BcoDI GTCTC 2 cut(s) 539, 1866
BfaI CTAG 2 cut(s) 1805, 2132
BfmI CTRYAG 1 cut(s) 332
BfoI RGCGCY 3 cut(s) 213, 225, 381
BfuAI ACCTGC 2 cut(s) 800, 1757
BglI GCCNNNNNGGC 1 cut(s) 63
Bme1390I CCNGG 3 cut(s) 390, 391, 556
Bme18I GGWCC 6 cut(s) 365, 398, 425, 452, 479, 1883
BmeRI GACNNNNNGTC 1 cut(s) 766
BmeT110I CYCGRG 7 cut(s) 343, 361, 389, 475, 536, 1697, 1752
BmgT120I GGNCC 7 cut(s) 365, 398, 425, 452, 479, 887, 1883
BmrFI CCNGG 3 cut(s) 390, 391, 556
BmsI GCATC 7 cut(s) 167, 288, 477, 883, 1377, 1693, 2014
BpiI GAAGAC 2 cut(s) 605, 765
Bpu14I TTCGAA 2 cut(s) 1025, 1118
BpuEI CTTGAG 1 cut(s) 1366
BpuMI CCSGG 3 cut(s) 390, 391, 556
Bsa29I ATCGAT 2 cut(s) 258, 282
BsaAI YACGTR 1 cut(s) 829
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 5 cut(s) 389, 512, 1162, 1641, 1698
BsaWI WCCGGW 3 cut(s) 449, 1060, 1292
BsaXI ACNNNNNCTCC 5 cut(s) 836, 866, 1840, 1870, 2140
Bsc4I CCNNNNNNNGG 6 cut(s) 374, 395, 1168, 1521, 1732, 1893
Bse118I RCCGGY 1 cut(s) 38
Bse3DI GCAATG 3 cut(s) 984, 1612, 1976
BseCI ATCGAT 2 cut(s) 258, 282
BseDI CCNNGG 5 cut(s) 389, 512, 1162, 1641, 1698
BseGI GGATG 9 cut(s) 158, 339, 967, 1075, 1324, 1392, 1462, 1592, 1708
BseLI CCNNNNNNNGG 6 cut(s) 374, 395, 1168, 1521, 1732, 1893
BseMI GCAATG 3 cut(s) 984, 1612, 1976
BseMII CTCAG 1 cut(s) 1273
BseRI GAGGAG 4 cut(s) 857, 1141, 1210, 1373
BseSI GKGCMC 1 cut(s) 828
BseXI GCAGC 5 cut(s) 277, 346, 738, 2014, 2020
BseYI CCCAGC 2 cut(s) 145, 380
BsgI GTGCAG 1 cut(s) 1785
Bsh1236I CGCG 3 cut(s) 438, 648, 1164
Bsh1285I CGRYCG 4 cut(s) 286, 432, 463, 528
BshFI GGCC 5 cut(s) 54, 117, 888, 1364, 2045
BshNI GGYRCC 2 cut(s) 980, 2097
BshVI ATCGAT 2 cut(s) 258, 282
BsiEI CGRYCG 4 cut(s) 286, 432, 463, 528
BsiHKAI GWGCWC 2 cut(s) 308, 828
BsiHKCI CYCGRG 7 cut(s) 343, 361, 389, 475, 536, 1697, 1752
BslFI GGGAC 3 cut(s) 123, 1846, 1869
BslI CCNNNNNNNGG 6 cut(s) 374, 395, 1168, 1521, 1732, 1893
BsmAI GTCTC 2 cut(s) 539, 1866
BsmBI CGTCTC 1 cut(s) 1866
BsmFI GGGAC 3 cut(s) 123, 1846, 1869
BsmI GAATGC 1 cut(s) 1757
BsnI GGCC 5 cut(s) 54, 117, 888, 1364, 2045
Bso31I GGTCTC 1 cut(s) 539
BsoBI CYCGRG 7 cut(s) 343, 361, 389, 475, 536, 1697, 1752
Bsp119I TTCGAA 2 cut(s) 1025, 1118
Bsp1286I GDGCHC 3 cut(s) 308, 828, 1739
Bsp1407I TGTACA 1 cut(s) 921
BspANI GGCC 5 cut(s) 54, 117, 888, 1364, 2045
BspCNI CTCAG 1 cut(s) 1272
BspDI ATCGAT 2 cut(s) 258, 282
BspFNI CGCG 3 cut(s) 438, 648, 1164
BspMAI CTGCAG 1 cut(s) 336
BspMI ACCTGC 2 cut(s) 800, 1757
BspPI GGATC 5 cut(s) 546, 1291, 1653, 1988, 2094
BspT104I TTCGAA 2 cut(s) 1025, 1118
BspT107I GGYRCC 2 cut(s) 980, 2097
BspTNI GGTCTC 1 cut(s) 539
BsrBI CCGCTC 1 cut(s) 27
BsrDI GCAATG 3 cut(s) 984, 1612, 1976
BsrFI RCCGGY 1 cut(s) 38
BsrGI TGTACA 1 cut(s) 921
BssAI RCCGGY 1 cut(s) 38
BssECI CCNNGG 5 cut(s) 389, 512, 1162, 1641, 1698
BssNI GRCGYC 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 721, 1727
Bst6I CTCTTC 1 cut(s) 1659
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 1763
BstAUI TGTACA 1 cut(s) 921
BstBAI YACGTR 1 cut(s) 829
BstBI TTCGAA 2 cut(s) 1025, 1118
BstC8I GCNNGC 4 cut(s) 156, 566, 588, 1708
BstDEI CTNAG 1 cut(s) 1259
BstDSI CCRYGG 2 cut(s) 1162, 1641
BstF5I GGATG 9 cut(s) 158, 339, 967, 1075, 1324, 1392, 1462, 1592, 1708
BstFNI CGCG 3 cut(s) 438, 648, 1164
BstH2I RGCGCY 3 cut(s) 213, 225, 381
BstHHI GCGC 9 cut(s) 212, 224, 380, 438, 799, 898, 1501, 1712, 2002
BstMAI GTCTC 2 cut(s) 539, 1866
BstMCI CGRYCG 4 cut(s) 286, 432, 463, 528
BstSCI CCNGG 3 cut(s) 388, 389, 554
BstSFI CTRYAG 1 cut(s) 332
BstSLI GKGCMC 1 cut(s) 828
BstUI CGCG 3 cut(s) 438, 648, 1164
BstV1I GCAGC 5 cut(s) 277, 346, 738, 2014, 2020
BstV2I GAAGAC 2 cut(s) 605, 765
BstX2I RGATCY 2 cut(s) 551, 1993
BstYI RGATCY 2 cut(s) 551, 1993
Bsu15I ATCGAT 2 cut(s) 258, 282
BsuRI GGCC 5 cut(s) 54, 117, 888, 1364, 2045
BsuTUI ATCGAT 2 cut(s) 258, 282
BtgI CCRYGG 2 cut(s) 1162, 1641
BtgZI GCGATG 1 cut(s) 610
BtsCI GGATG 9 cut(s) 158, 339, 967, 1075, 1324, 1392, 1462, 1592, 1708
BtsI GCAGTG 1 cut(s) 329
BtsIMutI CAGTG 2 cut(s) 329, 797
BveI ACCTGC 2 cut(s) 800, 1757
Cac8I GCNNGC 4 cut(s) 156, 566, 588, 1708
CaiI CAGNNNCTG 1 cut(s) 2008
CfoI GCGC 9 cut(s) 212, 224, 380, 438, 799, 898, 1501, 1712, 2002
Cfr10I RCCGGY 1 cut(s) 38
Cfr13I GGNCC 7 cut(s) 365, 398, 425, 452, 479, 887, 1883
Cfr42I CCGCGG 1 cut(s) 1165
Cfr9I CCCGGG 1 cut(s) 389
ClaI ATCGAT 2 cut(s) 258, 282
CseI GACGC 2 cut(s) 31, 637
Csp6I GTAC 4 cut(s) 922, 1159, 1843, 2098
CviAII CATG 3 cut(s) 445, 2102, 2154
CviQI GTAC 4 cut(s) 922, 1159, 1843, 2098
DdeI CTNAG 1 cut(s) 1259
DraI TTTAAA 1 cut(s) 544
DriI GACNNNNNGTC 1 cut(s) 766
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 1659
Eam1105I GACNNNNNGTC 1 cut(s) 766
EarI CTCTTC 1 cut(s) 1659
EciI GGCGGA 3 cut(s) 699, 986, 1523
Ecl136II GAGCTC 1 cut(s) 306
Eco147I AGGCCT 1 cut(s) 1364
Eco24I GRGCYC 2 cut(s) 308, 1739
Eco31I GGTCTC 1 cut(s) 539
Eco32I GATATC 1 cut(s) 594
Eco47I GGWCC 6 cut(s) 365, 398, 425, 452, 479, 1883
Eco53kI GAGCTC 1 cut(s) 306
Eco57I CTGAAG 4 cut(s) 178, 773, 1359, 2007
Eco88I CYCGRG 7 cut(s) 343, 361, 389, 475, 536, 1697, 1752
EcoICRI GAGCTC 1 cut(s) 306
EcoO109I RGGNCCY 3 cut(s) 398, 479, 1883
EcoRI GAATTC 2 cut(s) 1027, 1120
EcoRV GATATC 1 cut(s) 594
EcoT38I GRGCYC 2 cut(s) 308, 1739
Esp3I CGTCTC 1 cut(s) 1866
FaeI CATG 3 cut(s) 448, 2105, 2157
FalI AAGNNNNNCTT 2 cut(s) 1950, 1982
FaqI GGGAC 3 cut(s) 123, 1846, 1869
FatI CATG 3 cut(s) 444, 2101, 2153
FauI CCCGC 2 cut(s) 20, 431
FbaI TGATCA 1 cut(s) 354
FokI GGATG 9 cut(s) 145, 326, 974, 1082, 1331, 1399, 1469, 1599, 1715
FriOI GRGCYC 2 cut(s) 308, 1739
FspBI CTAG 2 cut(s) 1805, 2132
GlaI GCGC 9 cut(s) 211, 223, 379, 437, 798, 897, 1500, 1711, 2001
GsaI CCCAGC 2 cut(s) 149, 384
HaeII RGCGCY 3 cut(s) 213, 225, 381
HaeIII GGCC 5 cut(s) 54, 117, 888, 1364, 2045
HgaI GACGC 2 cut(s) 31, 637
HhaI GCGC 9 cut(s) 212, 224, 380, 438, 799, 898, 1501, 1712, 2002
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 3 cut(s) 448, 2105, 2157
Hin6I GCGC 9 cut(s) 210, 222, 378, 436, 797, 896, 1499, 1710, 2000
HinP1I GCGC 9 cut(s) 210, 222, 378, 436, 797, 896, 1499, 1710, 2000
HindIII AAGCTT 1 cut(s) 566
HphI GGTGA 4 cut(s) 28, 824, 1055, 1463
Hpy166II GTNNAC 6 cut(s) 172, 455, 826, 924, 934, 1290
Hpy188III TCNNGA 8 cut(s) 16, 301, 343, 523, 549, 865, 1403, 1805
Hpy8I GTNNAC 6 cut(s) 172, 455, 826, 924, 934, 1290
Hpy99I CGWCG 6 cut(s) 77, 107, 653, 767, 866, 878
HpyCH4III ACNGT 2 cut(s) 721, 1727
HpyCH4IV ACGT 1 cut(s) 828
HpyCH4V TGCA 8 cut(s) 334, 826, 998, 1094, 1766, 1799, 1981, 2036
HpyF3I CTNAG 1 cut(s) 1259
HpySE526I ACGT 1 cut(s) 828
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 3 cut(s) 448, 2105, 2157
HspAI GCGC 9 cut(s) 210, 222, 378, 436, 797, 896, 1499, 1710, 2000
KpnI GGTACC 1 cut(s) 2101
Ksp22I TGATCA 1 cut(s) 354
KspI CCGCGG 1 cut(s) 1165
LmnI GCTCC 5 cut(s) 311, 516, 940, 1019, 1295
Lsp1109I GCAGC 5 cut(s) 277, 346, 738, 2014, 2020
LweI GCATC 7 cut(s) 167, 288, 477, 883, 1377, 1693, 2014
MaeI CTAG 2 cut(s) 1805, 2132
MaeII ACGT 1 cut(s) 828
MaeIII GTNAC 3 cut(s) 59, 494, 1747
MbiI CCGCTC 1 cut(s) 27
MflI RGATCY 2 cut(s) 551, 1993
MhlI GDGCHC 3 cut(s) 308, 828, 1739
MluCI AATT 9 cut(s) 712, 733, 1027, 1081, 1108, 1120, 1144, 1406, 1618
MlyI GAGTC 4 cut(s) 86, 105, 776, 787
MmeI TCCRAC 1 cut(s) 160
MroXI GAANNNNTTC 1 cut(s) 11
MseI TTAA 6 cut(s) 543, 699, 882, 945, 1247, 1557
MslI CAYNNNNRTG 4 cut(s) 629, 929, 1317, 2106
MspA1I CMGCKG 2 cut(s) 1164, 1636
MspR9I CCNGG 3 cut(s) 390, 391, 556
Mva1269I GAATGC 1 cut(s) 1757
MvnI CGCG 3 cut(s) 438, 648, 1164
NciI CCSGG 3 cut(s) 390, 391, 556
NlaIII CATG 3 cut(s) 448, 2105, 2157
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 2 cut(s) 59, 1747
NspV TTCGAA 2 cut(s) 1025, 1118
OliI CACNNNNGTG 1 cut(s) 929
PaqCI CACCTGC 1 cut(s) 800
PceI AGGCCT 1 cut(s) 1364
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 870
PctI GAATGC 1 cut(s) 1757
PdmI GAANNNNTTC 1 cut(s) 11
PflFI GACNNNGTC 1 cut(s) 1727
PfoI TCCNGGA 1 cut(s) 554
Ple19I CGATCG 4 cut(s) 286, 432, 463, 528
PleI GAGTC 4 cut(s) 85, 105, 775, 786
PpsI GAGTC 4 cut(s) 85, 105, 775, 786
Ppu21I YACGTR 1 cut(s) 829
PpuMI RGGWCCY 3 cut(s) 398, 479, 1883
PshBI ATTAAT 2 cut(s) 699, 1557
Psp124BI GAGCTC 1 cut(s) 308
Psp5II RGGWCCY 3 cut(s) 398, 479, 1883
PspFI CCCAGC 2 cut(s) 145, 380
PspPI GGNCC 7 cut(s) 365, 398, 425, 452, 479, 887, 1883
PspPPI RGGWCCY 3 cut(s) 398, 479, 1883
PstI CTGCAG 1 cut(s) 336
PstNI CAGNNNCTG 1 cut(s) 2008
PsuI RGATCY 2 cut(s) 551, 1993
PsyI GACNNNGTC 1 cut(s) 1727
PvuI CGATCG 4 cut(s) 286, 432, 463, 528
PvuII CAGCTG 1 cut(s) 1636
RsaI GTAC 4 cut(s) 923, 1160, 1844, 2099
RsaNI GTAC 4 cut(s) 922, 1159, 1843, 2098
RseI CAYNNNNRTG 4 cut(s) 629, 929, 1317, 2106
SacI GAGCTC 1 cut(s) 308
SacII CCGCGG 1 cut(s) 1165
SaqAI TTAA 6 cut(s) 543, 699, 882, 945, 1247, 1557
Sau96I GGNCC 7 cut(s) 365, 398, 425, 452, 479, 887, 1883
SchI GAGTC 4 cut(s) 86, 105, 776, 787
ScrFI CCNGG 3 cut(s) 390, 391, 556
SduI GDGCHC 3 cut(s) 308, 828, 1739
SfaNI GCATC 7 cut(s) 167, 288, 477, 883, 1377, 1693, 2014
SfcI CTRYAG 1 cut(s) 332
Sfr303I CCGCGG 1 cut(s) 1165
SfuI TTCGAA 2 cut(s) 1025, 1118
SgrAI CRCCGGYG 1 cut(s) 38
SgrBI CCGCGG 1 cut(s) 1165
SinI GGWCC 6 cut(s) 365, 398, 425, 452, 479, 1883
SmaI CCCGGG 1 cut(s) 391
SmiMI CAYNNNNRTG 4 cut(s) 629, 929, 1317, 2106
SmlI CTYRAG 1 cut(s) 1345
SmoI CTYRAG 1 cut(s) 1345
Sse9I AATT 9 cut(s) 712, 733, 1027, 1081, 1108, 1120, 1144, 1406, 1618
SseBI AGGCCT 1 cut(s) 1364
SspMI CTAG 2 cut(s) 1805, 2132
SstI GAGCTC 1 cut(s) 308
StuI AGGCCT 1 cut(s) 1364
StyD4I CCNGG 3 cut(s) 388, 389, 554
TaaI ACNGT 2 cut(s) 721, 1727
TaiI ACGT 1 cut(s) 831
TaqII GACCGA 1 cut(s) 646
TasI AATT 9 cut(s) 712, 733, 1027, 1081, 1108, 1120, 1144, 1406, 1618
TatI WGTACW 1 cut(s) 921
TauI GCSGC 6 cut(s) 57, 296, 648, 684, 992, 1167
Tru1I TTAA 6 cut(s) 543, 699, 882, 945, 1247, 1557
Tru9I TTAA 6 cut(s) 543, 699, 882, 945, 1247, 1557
TscAI CASTG 2 cut(s) 336, 797
TseFI GTSAC 2 cut(s) 59, 1747
TseI GCWGC 5 cut(s) 290, 334, 726, 2002, 2033
Tsp45I GTSAC 2 cut(s) 59, 1747
TspDTI ATGAA 6 cut(s) 433, 1460, 1484, 1704, 1733, 1960
TspGWI ACGGA 4 cut(s) 205, 891, 1658, 1848
TspMI CCCGGG 1 cut(s) 389
TspRI CASTG 2 cut(s) 336, 797
Tth111I GACNNNGTC 1 cut(s) 1727
VneI GTGCAC 1 cut(s) 824
VpaK11BI GGWCC 6 cut(s) 365, 398, 425, 452, 479, 1883
VspI ATTAAT 2 cut(s) 699, 1557
XapI RAATTY 6 cut(s) 712, 733, 1027, 1108, 1120, 1144
XbaI TCTAGA 1 cut(s) 1804
XmaI CCCGGG 1 cut(s) 389
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 2 cut(s) 1805, 2132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.