Rw5G039770

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010729
Physical Location & Seq
Reverse (-)
70295160 .. 70298030
2871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039770.1

Sequence Viewer

Length: 2211 bp
ATGGGCAACAGCTTTGGAAAAGTTAGCGTCTGCTTCACCGGCGCTAAAGGAGTTCATCGTCGAACCCAAGATGAACCGCTTGTATTATCCGACACCTACGACGAGGGCCTCGGCCACTCTTTCTGCTATGTCCGACCCGACCCGACCTGGCTGGCCTCTTCTAAGGTCCACTCCGAGGAGGCCACCACCTTCAAAACCATCTCCGGAGCTTCGGTCAGCGCCAACACGTCGACGCCTCTGTCCACGGCCGTCGTCGATCTCTACTCTTACAACAGCATCGATCGGGCGGCGGCTTTCGAGTCCTCCACCTCTTTCGCCTCGATTCCGCTGCAGCCCATCCCGCGAAATCTCATCAATTCGGGTCCGATTTCGGGGAACTTCGGAGCGGGGATCCCCGGGTCGGGTCCGCTCGAGCGAGGGTTCCTGTCCGGCCCAATCGAGCGGGCGTTCATGTCCGGCCCGCTCGACCGTGGGCTGTTCTCCGGGCCCATGGAGAAGGGGTACTCGGAGCAGTTTCAGAGGAGCTTCTCCCACGGCGGCGGGTTCGCTTTCAAGCCGAGATCGGGGAAGCGGAAACTGATTCGGGTTCTTCAGAGAGCGATATCGAAGACGATATATCGCGGCCAGAACTCGATTCTGGCGCCGATTAAAAACCGCGTCGTTTCGGTGAAAGATCAGGATTGGATCGTCGGCGGCGGCGGCGGCGGAGGACTACAAGATAGTAAGCTTCAAAATCACAACGAGAATTTGACCGTGAGCTCTGTGAATTTCAGCAGTGAGGGGAGTTTGGAGGACGACGAGTCGTTGTCGAGTCAAAATCTTCAGTGGGCGCAAGGGAAAGCAGGGGAGGATCGAGTCCACGTCGTCGTTTCGGAGGAGGACGGCTGGGTTTTCGTCGGAATCTACGATGGATTCAACGGCCCAGATGCGCCGGATTATCTTCTATCGAATCTGTACACAGCTGTCCATAAGGAGCTGAAAGGTTTGATTTGGGACGAGAGGCTTGAATCAGCTGCGGTGGCCGCGCCTGCGACTTCCCCTGTTCACCCAGAATATTCGGATTCGGTTTCGGTTTCGATTTCGATTTCGGATAGTGATTTGGGAAGGAATCAAGTGGCAGATGCTTGTTCTCAGTTTGTTGAGGAAGAGAATTATCCTTGTGCAAGTGGGGATGTGAATTTCGATTCCAATTCCAAGAAAAGGGAGTCCAGGAACCCGAAAACAAAGTACCGGAGCTCCGGTCGGAAATGGGAGGATAATCAGAGGAGGTGGAGGTGTGAATGGGATAGAGAGAGAGTTGAGCTTGATCGGAGATTGAAGGAACAGCTTAATAGGTCCAGCGCAGATGGGTCATCGGCTACTAACCACTCTGATGTTTTGAAGGCTTTGTCTCAAGCTTTGAGGAAAACAGAGGAGGCCTATTTGGAAGTAGCGGATAAAATGCTCTTGGAAAATCCTGAGTTGGCCTTGATGGGGTCTTGTGTTCTTGTGATGTTGATGAAGGGGGAGGATGTTTATGTGATGAATGTGGGTGATAGTCGGGTTGTTTTGGCCCAGAAGGCGGAACCGGATTATTGGCTTGGGAAGATTAGGCAGGATTTGGAGAGGATTAATGAGGAAACATTACATGATCTTGAGGACGGCGAAAGACAGAATGCAATTCCCAATTTGAGCGCTGTTCAGCTGACGATGGATCACAGTACTAGCGAGCAAGAGGAAGTTGATAGAATCCGAAATGAACACCCAGAGGATGCTTGTGCTGTCATGAATGATCGTGTTAAAGGTTCATTGAAGGTCACTAGAGCTTTTGGTGCCGGCTTTCTCAAACAGCCTAAATGGAACAGTGCACTTCTAGAGGCATTCCAAATTGATTATATAGGGACTTCCCCCTACATCACTTGCTTCCCATATCTCCATCATCACAGATTAGGCCCTAAAGACAGGTTCTTGATATTATCCTCGGATGGGTTGTATCAGTACTTCACGAACGAAGAAGCTGTTTCTGAAGTTGAACTTTTCATCACATTGCAACCTGAAGGAGATCCGGCACAGCATCTTGTAGAGGAAGTATTGTTTCGTGCTGCCAAAAAAGCTGGTATGGACTTCCACGAATTGCTTCAAATACCACAAGGGGATCGGAGGCGGTACCATGATGATGTTTCCATCATCGTTATTTCTTTAGAGGGAAGAATATGGAGATCATGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0071944 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

736

Amino Acids

81.71

Weight (kDa)

5.36

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 442 - 678 2e-29 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07630 AT2G28890
fragaria_vesca FvH4_3g35990 FvH4_3g35990 FvH4_3g35990
malus_domestica MD03G1094800.v1.1 MD11G1110000.v1.1
prunus_persica Prupe.6G078500_v2.0.a1
pyrus_communis pycom03g07750 pycom11g09070
rosa_chinensis RchiOBHm_Chr5g0064391
rosa_laevigata RLG00000035720
rosa_multiflora Rmu_sc0000806.1_g000039
rosa_roxburghii Rroxscaffold_1G00016610
rosa_rugosa Rorug05G0364300
rosa_samantha Rh5AG422600 Rh5BG438400 Rh5CG461100 Rh5DG451600
rosa_wichuraiana Rw5G039770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 238
Acc65I GGTACC 1 cut(s) 2145
AccB1I GGYRCC 3 cut(s) 640, 1811, 2145
AccBSI CCGCTC 4 cut(s) 386, 409, 442, 463
AccI GTMKAC 1 cut(s) 230
AccII CGCG 4 cut(s) 343, 621, 657, 1025
AccIII TCCGGA 1 cut(s) 203
AclWI GGATC 7 cut(s) 385, 398, 692, 858, 1701, 2036, 2142
AcoI YGGCCR 4 cut(s) 112, 246, 622, 1020
AcsI RAATTY 3 cut(s) 745, 766, 1177
AcuI CTGAAG 4 cut(s) 575, 806, 2025, 2055
AcyI GRCGYC 2 cut(s) 233, 641
AfaI GTAC 6 cut(s) 503, 956, 1229, 1702, 1979, 2147
AfeI AGCGCT 1 cut(s) 1675
AfiI CCNNNNNNNGG 9 cut(s) 175, 371, 400, 401, 563, 1200, 1473, 1561, 1965
AflIII ACRYGT 1 cut(s) 225
AhdI GACNNNNNGTC 1 cut(s) 799
AjiI CACGTC 2 cut(s) 228, 862
AjnI CCWGG 2 cut(s) 146, 1208
Alw21I GWGCWC 3 cut(s) 761, 1238, 1849
Alw26I GTCTC 1 cut(s) 1395
Alw44I GTGCAC 1 cut(s) 1845
AlwI GGATC 7 cut(s) 385, 398, 692, 858, 1701, 2036, 2142
Ama87I CYCGRG 2 cut(s) 395, 410
Aor13HI TCCGGA 1 cut(s) 203
Aor51HI AGCGCT 1 cut(s) 1675
ApaI GGGCCC 1 cut(s) 489
ApaLI GTGCAC 1 cut(s) 1845
ApeKI GCWGC 4 cut(s) 328, 331, 1013, 2081
ApoI RAATTY 3 cut(s) 745, 766, 1177
AseI ATTAAT 1 cut(s) 1611
Asp700I GAANNNNTTC 1 cut(s) 2115
Asp718I GGTACC 1 cut(s) 2145
AspLEI GCGC 8 cut(s) 44, 221, 643, 832, 931, 1027, 1343, 1676
AsuC2I CCSGG 3 cut(s) 396, 397, 484
AsuHPI GGTGA 4 cut(s) 28, 679, 1037, 1544
AvaI CYCGRG 2 cut(s) 395, 410
AvaII GGWCC 4 cut(s) 166, 362, 404, 1335
BaeGI GKGCMC 2 cut(s) 489, 1849
BamHI GGATCC 1 cut(s) 390
BanI GGYRCC 3 cut(s) 640, 1811, 2145
BanII GRGCYC 3 cut(s) 489, 761, 1238
BarI GAAGNNNNNNTAC 2 cut(s) 1964, 1996
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 3 cut(s) 761, 1238, 1849
BbvI GCAGC 4 cut(s) 315, 343, 1000, 2068
BccI CCATC 9 cut(s) 206, 344, 902, 1340, 1465, 1684, 1923, 1958, 2171
BceAI ACGGC 6 cut(s) 233, 261, 550, 898, 934, 1657
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 2 cut(s) 148, 1210
BcnI CCSGG 3 cut(s) 396, 397, 484
BcoDI GTCTC 1 cut(s) 1395
BfaI CTAG 3 cut(s) 1704, 1800, 1853
BfmI CTRYAG 1 cut(s) 329
BfoI RGCGCY 4 cut(s) 45, 222, 644, 1677
BglI GCCNNNNNGGC 1 cut(s) 1559
BmcAI AGTACT 2 cut(s) 1702, 1979
Bme1390I CCNGG 5 cut(s) 148, 396, 397, 484, 1210
Bme18I GGWCC 4 cut(s) 166, 362, 404, 1335
BmeRI GACNNNNNGTC 1 cut(s) 799
BmeT110I CYCGRG 2 cut(s) 395, 410
BmgBI CACGTC 2 cut(s) 228, 862
BmrFI CCNGG 5 cut(s) 148, 396, 397, 484, 1210
BmsI GCATC 5 cut(s) 285, 916, 1111, 1741, 2062
BpiI GAAGAC 1 cut(s) 614
BpuEI CTTGAG 2 cut(s) 1377, 1655
BpuMI CCSGG 3 cut(s) 396, 397, 484
Bsa29I ATCGAT 1 cut(s) 279
BsaHI GRCGYC 2 cut(s) 233, 641
BsaJI CCNNGG 9 cut(s) 109, 174, 243, 394, 395, 469, 489, 532, 1959
BsaWI WCCGGW 4 cut(s) 203, 1230, 1238, 1567
BsaXI ACNNNNNCTCC 9 cut(s) 198, 228, 485, 515, 1220, 1225, 1250, 1255, 2188
Bsc4I CCNNNNNNNGG 9 cut(s) 175, 371, 400, 401, 563, 1200, 1473, 1561, 1965
Bse118I RCCGGY 2 cut(s) 38, 1814
Bse3DI GCAATG 1 cut(s) 2024
BseAI TCCGGA 1 cut(s) 203
BseBI CCWGG 2 cut(s) 148, 1210
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 9 cut(s) 109, 174, 243, 394, 395, 469, 489, 532, 1959
BseGI GGATG 5 cut(s) 336, 1177, 1516, 1756, 1969
BseLI CCNNNNNNNGG 9 cut(s) 175, 371, 400, 401, 563, 1200, 1473, 1561, 1965
BseMI GCAATG 1 cut(s) 2024
BseMII CTCAG 2 cut(s) 1145, 1449
BseRI GAGGAG 5 cut(s) 191, 535, 890, 1279, 1427
BseSI GKGCMC 2 cut(s) 489, 1849
BseX3I CGGCCG 1 cut(s) 246
BseXI GCAGC 4 cut(s) 315, 343, 1000, 2068
BseYI CCCAGC 1 cut(s) 885
Bsh1236I CGCG 4 cut(s) 343, 621, 657, 1025
Bsh1285I CGRYCG 4 cut(s) 249, 283, 469, 1243
BshNI GGYRCC 3 cut(s) 640, 1811, 2145
BshVI ATCGAT 1 cut(s) 279
BsiEI CGRYCG 4 cut(s) 249, 283, 469, 1243
BsiHKAI GWGCWC 3 cut(s) 761, 1238, 1849
BsiHKCI CYCGRG 2 cut(s) 395, 410
BslFI GGGAC 2 cut(s) 1007, 1894
BslI CCNNNNNNNGG 9 cut(s) 175, 371, 400, 401, 563, 1200, 1473, 1561, 1965
BsmAI GTCTC 1 cut(s) 1395
BsmFI GGGAC 2 cut(s) 1007, 1894
BsmI GAATGC 2 cut(s) 1660, 1859
BsoBI CYCGRG 2 cut(s) 395, 410
Bsp120I GGGCCC 1 cut(s) 485
Bsp1286I GDGCHC 4 cut(s) 489, 761, 1238, 1849
Bsp13I TCCGGA 1 cut(s) 203
Bsp1407I TGTACA 1 cut(s) 954
Bsp19I CCATGG 1 cut(s) 489
BspCNI CTCAG 2 cut(s) 1144, 1450
BspDI ATCGAT 1 cut(s) 279
BspEI TCCGGA 1 cut(s) 203
BspFNI CGCG 4 cut(s) 343, 621, 657, 1025
BspHI TCATGA 1 cut(s) 1764
BspMAI CTGCAG 1 cut(s) 333
BspPI GGATC 7 cut(s) 385, 398, 692, 858, 1701, 2036, 2142
BspT107I GGYRCC 3 cut(s) 640, 1811, 2145
BsrBI CCGCTC 4 cut(s) 386, 409, 442, 463
BsrDI GCAATG 1 cut(s) 2024
BsrFI RCCGGY 2 cut(s) 38, 1814
BsrGI TGTACA 1 cut(s) 954
BssAI RCCGGY 2 cut(s) 38, 1814
BssECI CCNNGG 9 cut(s) 109, 174, 243, 394, 395, 469, 489, 532, 1959
BssNI GRCGYC 2 cut(s) 233, 641
BssT1I CCWWGG 1 cut(s) 489
Bst2UI CCWGG 2 cut(s) 148, 1210
Bst4CI ACNGT 4 cut(s) 470, 754, 1700, 1844
Bst6I CTCTTC 2 cut(s) 163, 1140
BstACI GRCGYC 2 cut(s) 233, 641
BstAUI TGTACA 1 cut(s) 954
BstC8I GCNNGC 6 cut(s) 153, 444, 461, 1029, 1709, 1816
BstDEI CTNAG 3 cut(s) 162, 1131, 1458
BstDSI CCRYGG 4 cut(s) 243, 469, 489, 532
BstF5I GGATG 5 cut(s) 336, 1177, 1516, 1756, 1969
BstFNI CGCG 4 cut(s) 343, 621, 657, 1025
BstH2I RGCGCY 4 cut(s) 45, 222, 644, 1677
BstHHI GCGC 8 cut(s) 44, 221, 643, 832, 931, 1027, 1343, 1676
BstMAI GTCTC 1 cut(s) 1395
BstMCI CGRYCG 4 cut(s) 249, 283, 469, 1243
BstNI CCWGG 2 cut(s) 148, 1210
BstSCI CCNGG 5 cut(s) 146, 394, 395, 482, 1208
BstSFI CTRYAG 1 cut(s) 329
BstSLI GKGCMC 2 cut(s) 489, 1849
BstUI CGCG 4 cut(s) 343, 621, 657, 1025
BstV1I GCAGC 4 cut(s) 315, 343, 1000, 2068
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 2 cut(s) 390, 2041
BstYI RGATCY 2 cut(s) 390, 2041
BstZI CGGCCG 1 cut(s) 246
Bsu15I ATCGAT 1 cut(s) 279
BsuTUI ATCGAT 1 cut(s) 279
BtgI CCRYGG 4 cut(s) 243, 469, 489, 532
BtrI CACGTC 2 cut(s) 228, 862
BtsCI GGATG 5 cut(s) 336, 1177, 1516, 1756, 1969
BtsI GCAGTG 1 cut(s) 781
BtsIMutI CAGTG 3 cut(s) 781, 830, 1849
Cac8I GCNNGC 6 cut(s) 153, 444, 461, 1029, 1709, 1816
CciI TCATGA 1 cut(s) 1764
CfoI GCGC 8 cut(s) 44, 221, 643, 832, 931, 1027, 1343, 1676
Cfr10I RCCGGY 2 cut(s) 38, 1814
Cfr9I CCCGGG 1 cut(s) 395
ClaI ATCGAT 1 cut(s) 279
CseI GACGC 3 cut(s) 16, 241, 646
Csp6I GTAC 6 cut(s) 502, 955, 1228, 1701, 1978, 2146
CviAII CATG 6 cut(s) 451, 490, 1628, 1765, 2150, 2202
CviQI GTAC 6 cut(s) 502, 955, 1228, 1701, 1978, 2146
DdeI CTNAG 3 cut(s) 162, 1131, 1458
DinI GGCGCC 1 cut(s) 642
DrdI GACNNNNNNGTC 1 cut(s) 238
DriI GACNNNNNGTC 1 cut(s) 799
DseDI GACNNNNNNGTC 1 cut(s) 238
EaeI YGGCCR 4 cut(s) 112, 246, 622, 1020
EagI CGGCCG 1 cut(s) 246
Eam1104I CTCTTC 2 cut(s) 163, 1140
Eam1105I GACNNNNNGTC 1 cut(s) 799
EarI CTCTTC 2 cut(s) 163, 1140
EciI GGCGGA 2 cut(s) 720, 1577
Ecl136II GAGCTC 2 cut(s) 759, 1236
EclXI CGGCCG 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 489
Eco147I AGGCCT 1 cut(s) 1418
Eco24I GRGCYC 3 cut(s) 489, 761, 1238
Eco32I GATATC 1 cut(s) 603
Eco47I GGWCC 4 cut(s) 166, 362, 404, 1335
Eco47III AGCGCT 1 cut(s) 1675
Eco52I CGGCCG 1 cut(s) 246
Eco53kI GAGCTC 2 cut(s) 759, 1236
Eco57I CTGAAG 4 cut(s) 575, 806, 2025, 2055
Eco88I CYCGRG 2 cut(s) 395, 410
EcoICRI GAGCTC 2 cut(s) 759, 1236
EcoO109I RGGNCCY 2 cut(s) 106, 1931
EcoRII CCWGG 2 cut(s) 146, 1208
EcoRV GATATC 1 cut(s) 603
EcoT14I CCWWGG 1 cut(s) 489
EcoT38I GRGCYC 3 cut(s) 489, 761, 1238
EgeI GGCGCC 1 cut(s) 642
EheI GGCGCC 1 cut(s) 642
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 6 cut(s) 454, 493, 1631, 1768, 2153, 2205
FalI AAGNNNNNCTT 2 cut(s) 1998, 2030
FaqI GGGAC 2 cut(s) 1007, 1894
FatI CATG 6 cut(s) 450, 489, 1627, 1764, 2149, 2201
FauI CCCGC 5 cut(s) 348, 379, 435, 468, 533
FblI GTMKAC 1 cut(s) 230
FokI GGATG 5 cut(s) 323, 1184, 1523, 1763, 1976
FriOI GRGCYC 3 cut(s) 489, 761, 1238
FspBI CTAG 3 cut(s) 1704, 1800, 1853
GlaI GCGC 8 cut(s) 43, 220, 642, 831, 930, 1026, 1342, 1675
GsaI CCCAGC 1 cut(s) 889
HaeII RGCGCY 4 cut(s) 45, 222, 644, 1677
HgaI GACGC 3 cut(s) 16, 241, 646
HhaI GCGC 8 cut(s) 44, 221, 643, 832, 931, 1027, 1343, 1676
Hin1I GRCGYC 2 cut(s) 233, 641
Hin1II CATG 6 cut(s) 454, 493, 1631, 1768, 2153, 2205
Hin6I GCGC 8 cut(s) 42, 219, 641, 830, 929, 1025, 1341, 1674
HinP1I GCGC 8 cut(s) 42, 219, 641, 830, 929, 1025, 1341, 1674
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HindIII AAGCTT 2 cut(s) 725, 1395
HphI GGTGA 4 cut(s) 28, 679, 1037, 1544
Hpy166II GTNNAC 7 cut(s) 169, 231, 243, 859, 957, 1045, 1847
Hpy188III TCNNGA 8 cut(s) 204, 677, 1457, 1634, 1765, 1853, 1948, 1984
Hpy8I GTNNAC 7 cut(s) 169, 231, 243, 859, 957, 1045, 1847
HpyAV CCTTC 9 cut(s) 199, 490, 1098, 1312, 1375, 1495, 1552, 1786, 2030
HpyCH4III ACNGT 4 cut(s) 470, 754, 1700, 1844
HpyCH4IV ACGT 2 cut(s) 227, 861
HpyCH4V TGCA 5 cut(s) 331, 1163, 1658, 1847, 2029
HpyF3I CTNAG 3 cut(s) 162, 1131, 1458
HpySE526I ACGT 2 cut(s) 227, 861
Hsp92I GRCGYC 2 cut(s) 233, 641
Hsp92II CATG 6 cut(s) 454, 493, 1631, 1768, 2153, 2205
HspAI GCGC 8 cut(s) 42, 219, 641, 830, 929, 1025, 1341, 1674
KasI GGCGCC 1 cut(s) 640
Kpn2I TCCGGA 1 cut(s) 203
KpnI GGTACC 1 cut(s) 2149
KroI GCCGGC 1 cut(s) 1814
KroNI GCCGGC 1 cut(s) 1816
LmnI GCTCC 7 cut(s) 206, 383, 508, 522, 973, 1233, 1241
Lsp1109I GCAGC 4 cut(s) 315, 343, 1000, 2068
LweI GCATC 5 cut(s) 285, 916, 1111, 1741, 2062
MaeI CTAG 3 cut(s) 1704, 1800, 1853
MaeII ACGT 2 cut(s) 227, 861
MaeIII GTNAC 1 cut(s) 1795
MbiI CCGCTC 4 cut(s) 386, 409, 442, 463
MboII GAAGA 9 cut(s) 150, 581, 619, 812, 932, 1157, 1597, 2003, 2199
MflI RGATCY 2 cut(s) 390, 2041
MhlI GDGCHC 4 cut(s) 489, 761, 1238, 1849
Mly113I GGCGCC 1 cut(s) 641
MlyI GAGTC 5 cut(s) 308, 809, 820, 864, 1214
MmeI TCCRAC 4 cut(s) 114, 157, 877, 1223
MroI TCCGGA 1 cut(s) 203
MroNI GCCGGC 1 cut(s) 1814
MroXI GAANNNNTTC 1 cut(s) 2115
MseI TTAA 4 cut(s) 648, 1329, 1611, 1779
MslI CAYNNNNRTG 2 cut(s) 1371, 2154
MspA1I CMGCKG 4 cut(s) 328, 962, 1013, 1684
MspR9I CCNGG 5 cut(s) 148, 396, 397, 484, 1210
Mva1269I GAATGC 2 cut(s) 1660, 1859
MvaI CCWGG 2 cut(s) 148, 1210
MvnI CGCG 4 cut(s) 343, 621, 657, 1025
NaeI GCCGGC 1 cut(s) 1816
NarI GGCGCC 1 cut(s) 641
NciI CCSGG 3 cut(s) 396, 397, 484
NcoI CCATGG 1 cut(s) 489
NgoMIV GCCGGC 1 cut(s) 1814
NlaIII CATG 6 cut(s) 454, 493, 1631, 1768, 2153, 2205
NmeAIII GCCGAG 2 cut(s) 90, 582
NmuCI GTSAC 1 cut(s) 1795
PaeR7I CTCGAG 1 cut(s) 410
PagI TCATGA 1 cut(s) 1764
PceI AGGCCT 1 cut(s) 1418
PcsI WCGNNNNNNNCGW 1 cut(s) 903
PctI GAATGC 2 cut(s) 1660, 1859
PdiI GCCGGC 1 cut(s) 1816
PdmI GAANNNNTTC 1 cut(s) 2115
PfoI TCCNGGA 1 cut(s) 1208
Ple19I CGATCG 1 cut(s) 283
PleI GAGTC 5 cut(s) 307, 808, 819, 863, 1213
PluTI GGCGCC 1 cut(s) 644
PpsI GAGTC 5 cut(s) 307, 808, 819, 863, 1213
PshBI ATTAAT 1 cut(s) 1611
Psp124BI GAGCTC 2 cut(s) 761, 1238
Psp6I CCWGG 2 cut(s) 146, 1208
PspFI CCCAGC 1 cut(s) 885
PspGI CCWGG 2 cut(s) 146, 1208
PspOMI GGGCCC 1 cut(s) 485
PspXI VCTCGAGB 1 cut(s) 410
PstI CTGCAG 1 cut(s) 333
PsuI RGATCY 2 cut(s) 390, 2041
PvuI CGATCG 1 cut(s) 283
PvuII CAGCTG 3 cut(s) 962, 1013, 1684
RsaI GTAC 6 cut(s) 503, 956, 1229, 1702, 1979, 2147
RsaNI GTAC 6 cut(s) 502, 955, 1228, 1701, 1978, 2146
RseI CAYNNNNRTG 2 cut(s) 1371, 2154
SacI GAGCTC 2 cut(s) 761, 1238
SalI GTCGAC 1 cut(s) 229
SaqAI TTAA 4 cut(s) 648, 1329, 1611, 1779
ScaI AGTACT 2 cut(s) 1702, 1979
SchI GAGTC 5 cut(s) 308, 809, 820, 864, 1214
ScrFI CCNGG 5 cut(s) 148, 396, 397, 484, 1210
SduI GDGCHC 4 cut(s) 489, 761, 1238, 1849
SfaNI GCATC 5 cut(s) 285, 916, 1111, 1741, 2062
SfcI CTRYAG 1 cut(s) 329
SfoI GGCGCC 1 cut(s) 642
Sfr274I CTCGAG 1 cut(s) 410
SgrAI CRCCGGYG 1 cut(s) 38
SgrDI CGTCGACG 1 cut(s) 229
SinI GGWCC 4 cut(s) 166, 362, 404, 1335
SlaI CTCGAG 1 cut(s) 410
SmaI CCCGGG 1 cut(s) 397
SmiMI CAYNNNNRTG 2 cut(s) 1371, 2154
SmlI CTYRAG 3 cut(s) 410, 1392, 1634
SmoI CTYRAG 3 cut(s) 410, 1392, 1634
SseBI AGGCCT 1 cut(s) 1418
SspDI GGCGCC 1 cut(s) 640
SspI AATATT 1 cut(s) 1055
SspMI CTAG 3 cut(s) 1704, 1800, 1853
SstI GAGCTC 2 cut(s) 761, 1238
StuI AGGCCT 1 cut(s) 1418
StyD4I CCNGG 5 cut(s) 146, 394, 395, 482, 1208
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 4 cut(s) 470, 754, 1700, 1844
TaiI ACGT 2 cut(s) 230, 864
TaqII GACCGA 1 cut(s) 202
TatI WGTACW 3 cut(s) 954, 1700, 1977
TauI GCSGC 9 cut(s) 290, 293, 540, 624, 696, 699, 702, 705, 1025
Tru1I TTAA 4 cut(s) 648, 1329, 1611, 1779
Tru9I TTAA 4 cut(s) 648, 1329, 1611, 1779
TscAI CASTG 3 cut(s) 781, 830, 1849
TseFI GTSAC 1 cut(s) 1795
TseI GCWGC 4 cut(s) 328, 331, 1013, 2081
Tsp45I GTSAC 1 cut(s) 1795
TspDTI ATGAA 9 cut(s) 44, 87, 439, 1514, 1538, 1752, 1776, 1781, 2008
TspMI CCCGGG 1 cut(s) 395
TspRI CASTG 3 cut(s) 781, 830, 1849
VneI GTGCAC 1 cut(s) 1845
VpaK11BI GGWCC 4 cut(s) 166, 362, 404, 1335
VspI ATTAAT 1 cut(s) 1611
XapI RAATTY 3 cut(s) 745, 766, 1177
XbaI TCTAGA 1 cut(s) 1852
XhoI CTCGAG 1 cut(s) 410
XmaI CCCGGG 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 230
XmnI GAANNNNTTC 1 cut(s) 2115
XspI CTAG 3 cut(s) 1704, 1800, 1853
ZrmI AGTACT 2 cut(s) 1702, 1979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.