Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005806

Physical Location & Seq

Genomic Coordinates Forward (+)

10558635 .. 10564893

Gene Structure 6259 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1117300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2223 bp

ATGGCGACCGGAGCTGTACCGGCTTCTTTTACGGGTCTGAAAAGCAGGGACTCAAGTTTCGGGTTTGCTAAAAGTATGGATTTTGCGAGGGTTTCTGATTTGAAGAGGTTCAAGTCTGGCAGAACAAGAATCTCAGTGATACGAAACTCAAATCCAGGCTCGGATATTGCTGAGCTCAAGCCTGCATCTGAAGGCAGCCCTTTGTTAGTTCCTAGGCAAAAGTATTGTGAATCCATACACAAAACTGTCAGGAGGAAAACGCGCACAGTTATGGTTGGAAATGTGGCTATTGGTAGTGAGCATCCCATAAGGATTCAAACAATGACCACAACTGACACAAAGGATGTTGCTGCTACAGTTGAACAGGTAATGAGAATAGCAGATAAGGGAGCAGATATTGTTCGGATAACAGTTCAAGGGAGGAAAGAAGCAGATGCTTGTTTTGAAATTAAAAATTCCCTTGTGCAGAAAAATTATGATATACCTTTGGTGGCAGATATTCATTTTGCTCCTACTGTTGCACTGCGGGTCGCTGAATGCTTTGACAAAATTCGTGTCAACCCTGGAAATTTTGCTGATAGACGGGCCCAGTTTGAGAAGTTAGAGTACACGGAAGATGACTATCAGAAAGAACTTGAGCACATTGAGCAGGTTTTTACTCCATTGGTTGAAAAGTGTAAGAAGTATGGAAGAGCAATGCGTATTGGGACAAACCACGGGAGCCTTTCAGATCGTATAATGAGCTATTATGGGGATTCTCCTCGAGGAATGGTTGAATCTGCATTTGAGTTTGCAAGAATTTGCCGGAAGTTGGACTACCATAATTTTGTTTTTTCAATGAAAGCAAGCAACCCGGTTATCATGGTCCAGGCATATCGTTTGCTTGTAGCTGAAATGTATGTTCAAGGCTGGGATTATCCATTGCACTTGGGAGTTACTGAAGCTGGAGAAGGTGAGGATGGGCGAATGAAATCTGCAATTGGTATTGGAACCCTTCTTCAGGATGGCTTGGGTGATACAATTAGGGTTTCACTTACTGAAGCACCAGAGGAGGAGATAGATCCCTGCAGACGATTGGCCAACCTTGGTAAGAGAGCAGCGGATCTTCAGCAAGGAGTGGCTCCATTTGAAGAGAAGCACCGACATTATTTTGAATTTCAGCGTCGATCTGGTCAACTGCCAATACAAAAGGAGGGTGAAGAGGTGGATTATAGAGGTGTCTTGCACCGTGATGGCTCTGTTCTGATGTCAGTATCCCTTGACCAGTTGAAGACACCAGAGCTCCTGTACAAGTCACTAGCAACAAAACTTGTTCTCGGAATGCCATTTAAGGATCTTGCAACCGTTGATTCAATCTTATTGAGACAACTTCCACCTGTTGATGATGCTGATTCTCGGCTAGCTCTCAAAAGGTTGATAGATGTAAGTATGGGTGTTATAACACCATTGTCAGAACAGCTAACAAAGCCATTGCCCAATGCCATGGTTCTGGTAAATTCGAAGGAGTTATCAACTGGTGCATACAAGCTTTTGCCAGAAGGTACACGGGTGGTGGTCTCATTACATGGTGATGAACCCTATGAAGTGCTGGATATTCTCAAGGGCATTGATGCTACAATGATTCTCCATGATCTGCCCTTTGGTGAAGATAAAACAAGCCGAGTGCATGCTGCAAGGAGGCTATTCGAGTATCTGGGCGACAATGCTCTAAACTTACCTGTAATACACCATATTCAGTTTCCAAGTGGAATCCATGGGGATGACTTGGTTATCGGTGCTGGTACCAATGCGGGGGCCCTTTTAGTAGATGGACTTGGAGATGGTCTTCTATTGGAAGCCCCAGACAAGGACTTTGATTACCTGAGAAACACATCTTTCAATTTACTACAAGGATGTAGAATGCGGAATACAAAGACGGAGTATGTTTCATGCCCATCCTGTGGCCGAACTTTGTTTGATCTTCAAGAAATAAGTGCACAAATACGAGAGAAGACATCACACTTGCCTGGCGTTTCAATTGCGATCATGGGTTGCATTGTAAATGGACCCGGGGAGATGGCTGATGCAGACTTTGGGTATGTTGGTGGCGCTCCTGGAAAGATTGACCTCTACGTTGGGAAGACGGTGGTGAAGCGTGGAATTGAGATGGAGCATGCAACCGATGCCTTGATCCAGCTAATAAAAGATCACGGCCGCTGGGTTGACCCTCCTGCGGAAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006081 GO:0006082 GO:0006090 GO:0006629 GO:0006644 GO:0006720 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009240 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009627 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009862 GO:0009863 GO:0009941 GO:0009987 GO:0010033 GO:0014070 GO:0016491 GO:0016725 GO:0019288 GO:0019637 GO:0019682 GO:0019752 GO:0023052 GO:0031967 GO:0031975 GO:0032787 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046429 GO:0046490 GO:0046677 GO:0048037 GO:0050789 GO:0050794 GO:0050896 GO:0051536 GO:0051539 GO:0051540 GO:0051704 GO:0051707 GO:0051716 GO:0052592 GO:0055114 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0090407 GO:0098542 GO:1901135 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130

Pfam Domains

Protein Families

GcpE

Protein Analysis

741

Amino Acids

81.91

Weight (kDa)

6.07

Isoelectric Point (pI)

36.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GcpE	PF04551	87 - 356	5.2e-90	GcpE TIM-barrel domain
GcpE_C	PF26540	640 - 728	3.3e-24	GcpE protein C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005806)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60600 AT5G60600 AT5G60600 AT5G60600 AT5G60600
fragaria_vesca	FvH4_3g32460 FvH4_3g32460 FvH4_3g32460
malus_domestica	MD03G1117300.v1.1 MD11G1135000.v1.1
prunus_persica	Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1
pyrus_communis	pycom11g11210
rosa_chinensis	RchiOBHm_Chr5g0059471
rosa_laevigata	RLG00000035288
rosa_multiflora	Rmu_sc0000375.1_g000001 Rmu_sc0001977.1_g000002
rosa_roxburghii	Rroxscaffold_1G00021070
rosa_rugosa	Rorug05G0322800 Rorug05G0322900 Rorug05G0322900
rosa_samantha	Rh5BG398900 Rh5DG413800
rosa_wichuraiana	Rw5G036470 Rw5G036510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1439
AbsI	CCTCGAGG	1 cut(s)	762
Acc36I	ACCTGC	1 cut(s)	640
Acc65I	GGTACC	1 cut(s)	1781
AccB1I	GGYRCC	1 cut(s)	1781
AccB7I	CCANNNNNTGG	1 cut(s)	1940
AccII	CGCG	1 cut(s)	262
AciI	CCGC	6 cut(s)	526, 1100, 1790, 1903, 2194, 2213
AclWI	GGATC	4 cut(s)	1055, 1110, 1341, 2164
AcoI	YGGCCR	3 cut(s)	1077, 1942, 2191
AcsI	RAATTY	6 cut(s)	454, 549, 568, 798, 1154, 1495
AcuI	CTGAAG	5 cut(s)	210, 960, 983, 1059, 1091
AfaI	GTAC	5 cut(s)	18, 608, 1289, 1543, 1783
AfiI	CCNNNNNNNGG	6 cut(s)	811, 1000, 1791, 1846, 1940, 2212
AjnI	CCWGG	5 cut(s)	154, 562, 867, 2005, 2092
Alw21I	GWGCWC	4 cut(s)	177, 642, 1284, 1978
Alw26I	GTCTC	2 cut(s)	1357, 1561
Alw44I	GTGCAC	1 cut(s)	1974
AlwI	GGATC	4 cut(s)	1055, 1110, 1341, 2164
Ama87I	CYCGRG	2 cut(s)	762, 2048
AoxI	GGCC	5 cut(s)	585, 1077, 1794, 1942, 2191
ApaI	GGGCCC	2 cut(s)	589, 1798
ApaLI	GTGCAC	1 cut(s)	1974
ApeKI	GCWGC	4 cut(s)	195, 350, 1097, 1670
ApoI	RAATTY	6 cut(s)	454, 549, 568, 798, 1154, 1495
ArsI	GACNNNNNNTTYG	6 cut(s)	41, 73, 540, 572, 1835, 1867
Asp700I	GAANNNNTTC	1 cut(s)	107
Asp718I	GGTACC	1 cut(s)	1781
AspA2I	CCTAGG	1 cut(s)	212
AspLEI	GCGC	2 cut(s)	264, 2090
AspS9I	GGNCC	6 cut(s)	585, 586, 865, 1794, 1795, 2045
AsuC2I	CCSGG	3 cut(s)	854, 2049, 2050
AsuHPI	GGTGA	6 cut(s)	965, 1025, 1208, 1580, 1655, 2140
AsuII	TTCGAA	1 cut(s)	1499
AsuNHI	GCTAGC	1 cut(s)	1399
AvaI	CYCGRG	2 cut(s)	762, 2048
AvaII	GGWCC	2 cut(s)	865, 2045
AvrII	CCTAGG	1 cut(s)	212
BaeGI	GKGCMC	3 cut(s)	589, 1798, 1978
BaeI	ACNNNNGTAYC	2 cut(s)	1533, 1566
BalI	TGGCCA	1 cut(s)	1079
BanI	GGYRCC	1 cut(s)	1781
BanII	GRGCYC	4 cut(s)	177, 589, 1284, 1798
BarI	GAAGNNNNNNTAC	4 cut(s)	590, 622, 800, 832
BbsI	GAAGAC	4 cut(s)	1277, 1817, 1997, 2126
Bbv12I	GWGCWC	4 cut(s)	177, 642, 1284, 1978
BbvI	GCAGC	4 cut(s)	207, 337, 1109, 1657
BccI	CCATC	8 cut(s)	953, 998, 1226, 1802, 1814, 1942, 2050, 2140
BceAI	ACGGC	1 cut(s)	2206
BciT130I	CCWGG	5 cut(s)	156, 564, 869, 2007, 2094
BciVI	GTATCC	1 cut(s)	1264
BcnI	CCSGG	3 cut(s)	854, 2049, 2050
BcoDI	GTCTC	2 cut(s)	1357, 1561
BfaI	CTAG	3 cut(s)	213, 1298, 1400
BfmI	CTRYAG	2 cut(s)	354, 1066
BfoI	RGCGCY	1 cut(s)	2091
BfuAI	ACCTGC	1 cut(s)	640
BfuI	GTATCC	1 cut(s)	1264
BisI	GCNGC	5 cut(s)	196, 351, 1098, 1671, 2194
BlnI	CCTAGG	1 cut(s)	212
BlpI	GCTNAGC	1 cut(s)	171
BlsI	GCNGC	5 cut(s)	197, 352, 1099, 1672, 2195
Bme1390I	CCNGG	8 cut(s)	156, 564, 854, 869, 2007, 2049, 2050, 2094
Bme18I	GGWCC	2 cut(s)	865, 2045
BmeT110I	CYCGRG	2 cut(s)	762, 2048
BmgT120I	GGNCC	6 cut(s)	585, 586, 865, 1794, 1795, 2045
BmiI	GGNNCC	8 cut(s)	587, 722, 991, 1122, 1783, 1795, 1796, 2047
BmrFI	CCNGG	8 cut(s)	156, 564, 854, 869, 2007, 2049, 2050, 2094
BmrI	ACTGGG	1 cut(s)	583
BmsI	GCATC	7 cut(s)	194, 310, 424, 1375, 1600, 2053, 2152
BmtI	GCTAGC	1 cut(s)	1403
BmuI	ACTGGG	1 cut(s)	583
BpiI	GAAGAC	4 cut(s)	1277, 1817, 1997, 2126
BpmI	CTGGAG	1 cut(s)	966
Bpu1102I	GCTNAGC	1 cut(s)	171
Bpu14I	TTCGAA	1 cut(s)	1499
BpuEI	CTTGAG	4 cut(s)	37, 161, 656, 1583
BpuMI	CCSGG	3 cut(s)	854, 2049, 2050
BsaBI	GATNNNNATC	1 cut(s)	621
BsaI	GGTCTC	1 cut(s)	1561
BsaJI	CCNNGG	8 cut(s)	212, 562, 715, 1084, 1482, 1753, 2048, 2049
BsaWI	WCCGGW	1 cut(s)	8
BsaXI	ACNNNNNCTCC	2 cut(s)	1266, 1296
Bsc4I	CCNNNNNNNGG	6 cut(s)	811, 1000, 1791, 1846, 1940, 2212
Bse118I	RCCGGY	1 cut(s)	19
Bse1I	ACTGG	3 cut(s)	589, 1264, 1519
Bse3DI	GCAATG	3 cut(s)	702, 920, 1469
Bse8I	GATNNNNATC	1 cut(s)	621
BseBI	CCWGG	5 cut(s)	156, 564, 869, 2007, 2094
BseDI	CCNNGG	8 cut(s)	212, 562, 715, 1084, 1482, 1753, 2048, 2049
BseGI	GGATG	7 cut(s)	301, 349, 964, 1009, 1765, 1898, 1934
BseJI	GATNNNNATC	1 cut(s)	621
BseLI	CCNNNNNNNGG	6 cut(s)	811, 1000, 1791, 1846, 1940, 2212
BseMI	GCAATG	3 cut(s)	702, 920, 1469
BseMII	CTCAG	3 cut(s)	147, 162, 1853
BseNI	ACTGG	3 cut(s)	589, 1264, 1519
BseRI	GAGGAG	3 cut(s)	750, 1064, 1067
BseSI	GKGCMC	3 cut(s)	589, 1798, 1978
BseX3I	CGGCCG	1 cut(s)	2191
BseXI	GCAGC	4 cut(s)	207, 337, 1109, 1657
BseYI	CCCAGC	2 cut(s)	909, 2196
BsgI	GTGCAG	1 cut(s)	485
Bsh1236I	CGCG	1 cut(s)	262
Bsh1285I	CGRYCG	2 cut(s)	9, 2194
BshFI	GGCC	5 cut(s)	587, 1079, 1796, 1944, 2193
BshNI	GGYRCC	1 cut(s)	1781
BsiEI	CGRYCG	2 cut(s)	9, 2194
BsiHKAI	GWGCWC	4 cut(s)	177, 642, 1284, 1978
BsiHKCI	CYCGRG	2 cut(s)	762, 2048
BsiSI	CCGG	5 cut(s)	9, 20, 805, 854, 2049
BslFI	GGGAC	2 cut(s)	62, 721
BslI	CCNNNNNNNGG	6 cut(s)	811, 1000, 1791, 1846, 1940, 2212
BsmAI	GTCTC	2 cut(s)	1357, 1561
BsmFI	GGGAC	2 cut(s)	62, 721
BsmI	GAATGC	3 cut(s)	542, 1326, 1905
BsnI	GGCC	5 cut(s)	587, 1079, 1796, 1944, 2193
Bso31I	GGTCTC	1 cut(s)	1561
BsoBI	CYCGRG	2 cut(s)	762, 2048
Bsp119I	TTCGAA	1 cut(s)	1499
Bsp120I	GGGCCC	2 cut(s)	585, 1794
Bsp1286I	GDGCHC	6 cut(s)	177, 589, 642, 1284, 1798, 1978
Bsp1407I	TGTACA	1 cut(s)	1287
Bsp1720I	GCTNAGC	1 cut(s)	171
Bsp19I	CCATGG	2 cut(s)	1482, 1753
BspACI	CCGC	6 cut(s)	526, 1100, 1790, 1903, 2194, 2213
BspANI	GGCC	5 cut(s)	587, 1079, 1796, 1944, 2193
BspCNI	CTCAG	3 cut(s)	146, 163, 1854
BspFNI	CGCG	1 cut(s)	262
BspLI	GGNNCC	8 cut(s)	587, 722, 991, 1122, 1783, 1795, 1796, 2047
BspMAI	CTGCAG	1 cut(s)	1070
BspMI	ACCTGC	1 cut(s)	640
BspOI	GCTAGC	1 cut(s)	1403
BspPI	GGATC	4 cut(s)	1055, 1110, 1341, 2164
BspQI	GCTCTTC	1 cut(s)	685
BspT104I	TTCGAA	1 cut(s)	1499
BspT107I	GGYRCC	1 cut(s)	1781
BspTNI	GGTCTC	1 cut(s)	1561
BsrDI	GCAATG	3 cut(s)	702, 920, 1469
BsrFI	RCCGGY	1 cut(s)	19
BsrGI	TGTACA	1 cut(s)	1287
BsrI	ACTGG	3 cut(s)	589, 1264, 1519
BssAI	RCCGGY	1 cut(s)	19
BssECI	CCNNGG	8 cut(s)	212, 562, 715, 1084, 1482, 1753, 2048, 2049
BssT1I	CCWWGG	4 cut(s)	212, 1084, 1482, 1753
Bst2UI	CCWGG	5 cut(s)	156, 564, 869, 2007, 2094
Bst4CI	ACNGT	8 cut(s)	247, 268, 358, 412, 517, 1229, 1345, 2125
Bst6I	CTCTTC	5 cut(s)	98, 685, 1125, 1194, 2211
BstAPI	GCANNNNNTGC	1 cut(s)	2162
BstAUI	TGTACA	1 cut(s)	1287
BstBI	TTCGAA	1 cut(s)	1499
BstC8I	GCNNGC	5 cut(s)	183, 847, 1401, 1668, 2154
BstDEI	CTNAG	3 cut(s)	133, 171, 1862
BstDSI	CCRYGG	3 cut(s)	715, 1482, 1753
BstENI	CCTNNNNNAGG	1 cut(s)	998
BstF5I	GGATG	7 cut(s)	301, 349, 964, 1009, 1765, 1898, 1934
BstFNI	CGCG	1 cut(s)	262
BstH2I	RGCGCY	1 cut(s)	2091
BstHHI	GCGC	2 cut(s)	264, 2090
BstMAI	GTCTC	2 cut(s)	1357, 1561
BstMCI	CGRYCG	2 cut(s)	9, 2194
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 20, 646, 1465, 2162
BstNI	CCWGG	5 cut(s)	156, 564, 869, 2007, 2094
BstNSI	RCATGY	2 cut(s)	1670, 2156
BstSCI	CCNGG	8 cut(s)	154, 562, 852, 867, 2005, 2047, 2048, 2092
BstSFI	CTRYAG	2 cut(s)	354, 1066
BstSLI	GKGCMC	3 cut(s)	589, 1798, 1978
BstUI	CGCG	1 cut(s)	262
BstV1I	GCAGC	4 cut(s)	207, 337, 1109, 1657
BstV2I	GAAGAC	4 cut(s)	1277, 1817, 1997, 2126
BstX2I	RGATCY	3 cut(s)	1060, 1102, 1333
BstXI	CCANNNNNNTGG	2 cut(s)	1483, 1489
BstYI	RGATCY	3 cut(s)	1060, 1102, 1333
BstZI	CGGCCG	1 cut(s)	2191
BsuI	GTATCC	1 cut(s)	1264
BsuRI	GGCC	5 cut(s)	587, 1079, 1796, 1944, 2193
BtgI	CCRYGG	3 cut(s)	715, 1482, 1753
BtsCI	GGATG	7 cut(s)	301, 349, 964, 1009, 1765, 1898, 1934
BtsI	GCAGTG	1 cut(s)	521
BtsIMutI	CAGTG	2 cut(s)	141, 521
BveI	ACCTGC	1 cut(s)	640
Cac8I	GCNNGC	5 cut(s)	183, 847, 1401, 1668, 2154
CfoI	GCGC	2 cut(s)	264, 2090
Cfr10I	RCCGGY	1 cut(s)	19
Cfr13I	GGNCC	6 cut(s)	585, 586, 865, 1794, 1795, 2045
Cfr9I	CCCGGG	1 cut(s)	2048
CseI	GACGC	1 cut(s)	1151
Csp6I	GTAC	5 cut(s)	17, 607, 1288, 1542, 1782
CviAII	CATG	9 cut(s)	862, 1483, 1565, 1628, 1667, 1754, 1929, 2026, 2153
CviQI	GTAC	5 cut(s)	17, 607, 1288, 1542, 1782
DdeI	CTNAG	3 cut(s)	133, 171, 1862
EaeI	YGGCCR	3 cut(s)	1077, 1942, 2191
EagI	CGGCCG	1 cut(s)	2191
Eam1104I	CTCTTC	5 cut(s)	98, 685, 1125, 1194, 2211
EarI	CTCTTC	5 cut(s)	98, 685, 1125, 1194, 2211
Ecl136II	GAGCTC	2 cut(s)	175, 1282
EclXI	CGGCCG	1 cut(s)	2191
Eco130I	CCWWGG	4 cut(s)	212, 1084, 1482, 1753
Eco24I	GRGCYC	4 cut(s)	177, 589, 1284, 1798
Eco31I	GGTCTC	1 cut(s)	1561
Eco47I	GGWCC	2 cut(s)	865, 2045
Eco52I	CGGCCG	1 cut(s)	2191
Eco53kI	GAGCTC	2 cut(s)	175, 1282
Eco57I	CTGAAG	5 cut(s)	210, 960, 983, 1059, 1091
Eco88I	CYCGRG	2 cut(s)	762, 2048
EcoICRI	GAGCTC	2 cut(s)	175, 1282
EcoNI	CCTNNNNNAGG	1 cut(s)	998
EcoO109I	RGGNCCY	2 cut(s)	1794, 1795
EcoRII	CCWGG	5 cut(s)	154, 562, 867, 2005, 2092
EcoT14I	CCWWGG	4 cut(s)	212, 1084, 1482, 1753
EcoT38I	GRGCYC	4 cut(s)	177, 589, 1284, 1798
ErhI	CCWWGG	4 cut(s)	212, 1084, 1482, 1753
FaeI	CATG	9 cut(s)	865, 1486, 1568, 1631, 1670, 1757, 1932, 2029, 2156
FaqI	GGGAC	2 cut(s)	62, 721
FatI	CATG	9 cut(s)	861, 1482, 1564, 1627, 1666, 1753, 1928, 2025, 2152
FauI	CCCGC	2 cut(s)	519, 1783
Fnu4HI	GCNGC	5 cut(s)	196, 351, 1098, 1671, 2194
FokI	GGATG	7 cut(s)	288, 356, 971, 1016, 1772, 1905, 1921
FriOI	GRGCYC	4 cut(s)	177, 589, 1284, 1798
Fsp4HI	GCNGC	5 cut(s)	196, 351, 1098, 1671, 2194
FspBI	CTAG	3 cut(s)	213, 1298, 1400
GlaI	GCGC	2 cut(s)	263, 2089
GluI	GCNGC	5 cut(s)	196, 351, 1098, 1671, 2194
GsaI	CCCAGC	2 cut(s)	913, 2200
GsuI	CTGGAG	1 cut(s)	966
HaeII	RGCGCY	1 cut(s)	2091
HaeIII	GGCC	5 cut(s)	587, 1079, 1796, 1944, 2193
HapII	CCGG	5 cut(s)	9, 20, 805, 854, 2049
HgaI	GACGC	1 cut(s)	1151
HhaI	GCGC	2 cut(s)	264, 2090
Hin1II	CATG	9 cut(s)	865, 1486, 1568, 1631, 1670, 1757, 1932, 2029, 2156
Hin6I	GCGC	2 cut(s)	262, 2088
HinP1I	GCGC	2 cut(s)	262, 2088
HincII	GTYRAC	3 cut(s)	559, 1175, 2203
HindII	GTYRAC	3 cut(s)	559, 1175, 2203
HindIII	AAGCTT	1 cut(s)	1526
HpaII	CCGG	5 cut(s)	9, 20, 805, 854, 2049
HphI	GGTGA	6 cut(s)	965, 1025, 1208, 1580, 1655, 2140
Hpy166II	GTNNAC	6 cut(s)	559, 609, 1175, 1544, 1976, 2203
Hpy188III	TCNNGA	3 cut(s)	250, 1001, 1964
Hpy8I	GTNNAC	6 cut(s)	559, 609, 1175, 1544, 1976, 2203
Hpy99I	CGWCG	1 cut(s)	1167
HpyAV	CCTTC	5 cut(s)	185, 944, 1004, 1495, 1532
HpyCH4III	ACNGT	8 cut(s)	247, 268, 358, 412, 517, 1229, 1345, 2125
HpyCH4IV	ACGT	1 cut(s)	2112
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 20, 646, 1465, 2162
HpyF3I	CTNAG	3 cut(s)	133, 171, 1862
HpySE526I	ACGT	1 cut(s)	2112
Hsp92II	CATG	9 cut(s)	865, 1486, 1568, 1631, 1670, 1757, 1932, 2029, 2156
HspAI	GCGC	2 cut(s)	262, 2088
KpnI	GGTACC	1 cut(s)	1785
LguI	GCTCTTC	1 cut(s)	685
LmnI	GCTCC	8 cut(s)	11, 389, 514, 720, 1126, 1287, 2095, 2149
Lsp1109I	GCAGC	4 cut(s)	207, 337, 1109, 1657
LweI	GCATC	7 cut(s)	194, 310, 424, 1375, 1600, 2053, 2152
MaeI	CTAG	3 cut(s)	213, 1298, 1400
MaeII	ACGT	1 cut(s)	2112
MaeIII	GTNAC	2 cut(s)	934, 1293
MfeI	CAATTG	2 cut(s)	978, 2016
MflI	RGATCY	3 cut(s)	1060, 1102, 1333
MhlI	GDGCHC	6 cut(s)	177, 589, 642, 1284, 1798, 1978
MlsI	TGGCCA	1 cut(s)	1079
MluNI	TGGCCA	1 cut(s)	1079
MlyI	GAGTC	1 cut(s)	44
MmeI	TCCRAC	2 cut(s)	256, 792
Mox20I	TGGCCA	1 cut(s)	1079
MroXI	GAANNNNTTC	1 cut(s)	107
MscI	TGGCCA	1 cut(s)	1079
MseI	TTAA	2 cut(s)	450, 1329
MslI	CAYNNNNRTG	4 cut(s)	269, 1230, 1569, 1758
Msp20I	TGGCCA	1 cut(s)	1079
MspA1I	CMGCKG	2 cut(s)	1100, 2196
MspI	CCGG	5 cut(s)	9, 20, 805, 854, 2049
MspR9I	CCNGG	8 cut(s)	156, 564, 854, 869, 2007, 2049, 2050, 2094
MunI	CAATTG	2 cut(s)	978, 2016
Mva1269I	GAATGC	3 cut(s)	542, 1326, 1905
MvaI	CCWGG	5 cut(s)	156, 564, 869, 2007, 2094
MvnI	CGCG	1 cut(s)	262
MwoI	GCNNNNNNNGC	5 cut(s)	11, 20, 646, 1465, 2162
NciI	CCSGG	3 cut(s)	854, 2049, 2050
NcoI	CCATGG	2 cut(s)	1482, 1753
NheI	GCTAGC	1 cut(s)	1399
NlaIII	CATG	9 cut(s)	865, 1486, 1568, 1631, 1670, 1757, 1932, 2029, 2156
NlaIV	GGNNCC	8 cut(s)	587, 722, 991, 1122, 1783, 1795, 1796, 2047
NmeAIII	GCCGAG	2 cut(s)	1375, 1685
NmuCI	GTSAC	1 cut(s)	1293
NspI	RCATGY	2 cut(s)	1670, 2156
NspV	TTCGAA	1 cut(s)	1499
PaeI	GCATGC	2 cut(s)	1670, 2156
PaeR7I	CTCGAG	1 cut(s)	762
PciSI	GCTCTTC	1 cut(s)	685
PctI	GAATGC	3 cut(s)	542, 1326, 1905
PdmI	GAANNNNTTC	1 cut(s)	107
PfeI	GAWTC	9 cut(s)	129, 230, 313, 755, 776, 1349, 1391, 1621, 1749
PflMI	CCANNNNNTGG	1 cut(s)	1940
PfoI	TCCNGGA	1 cut(s)	2092
PkrI	GCNGC	5 cut(s)	197, 352, 1099, 1672, 2195
PleI	GAGTC	1 cut(s)	44
PpsI	GAGTC	1 cut(s)	44
PsiI	TTATAA	1 cut(s)	1439
Psp124BI	GAGCTC	2 cut(s)	177, 1284
Psp6I	CCWGG	5 cut(s)	154, 562, 867, 2005, 2092
PspFI	CCCAGC	2 cut(s)	909, 2196
PspGI	CCWGG	5 cut(s)	154, 562, 867, 2005, 2092
PspN4I	GGNNCC	8 cut(s)	587, 722, 991, 1122, 1783, 1795, 1796, 2047
PspOMI	GGGCCC	2 cut(s)	585, 1794
PspPI	GGNCC	6 cut(s)	585, 586, 865, 1794, 1795, 2045
PspXI	VCTCGAGB	1 cut(s)	762
PstI	CTGCAG	1 cut(s)	1070
PsuI	RGATCY	3 cut(s)	1060, 1102, 1333
RsaI	GTAC	5 cut(s)	18, 608, 1289, 1543, 1783
RsaNI	GTAC	5 cut(s)	17, 607, 1288, 1542, 1782
RseI	CAYNNNNRTG	4 cut(s)	269, 1230, 1569, 1758
SacI	GAGCTC	2 cut(s)	177, 1284
SapI	GCTCTTC	1 cut(s)	685
SaqAI	TTAA	2 cut(s)	450, 1329
SatI	GCNGC	5 cut(s)	196, 351, 1098, 1671, 2194
Sau96I	GGNCC	6 cut(s)	585, 586, 865, 1794, 1795, 2045
SchI	GAGTC	1 cut(s)	44
ScrFI	CCNGG	8 cut(s)	156, 564, 854, 869, 2007, 2049, 2050, 2094
SduI	GDGCHC	6 cut(s)	177, 589, 642, 1284, 1798, 1978
SfaNI	GCATC	7 cut(s)	194, 310, 424, 1375, 1600, 2053, 2152
SfcI	CTRYAG	2 cut(s)	354, 1066
Sfr274I	CTCGAG	1 cut(s)	762
SfuI	TTCGAA	1 cut(s)	1499
SinI	GGWCC	2 cut(s)	865, 2045
SlaI	CTCGAG	1 cut(s)	762
SmaI	CCCGGG	1 cut(s)	2050
SmiMI	CAYNNNNRTG	4 cut(s)	269, 1230, 1569, 1758
SmlI	CTYRAG	5 cut(s)	52, 176, 635, 762, 1598
SmoI	CTYRAG	5 cut(s)	52, 176, 635, 762, 1598
SphI	GCATGC	2 cut(s)	1670, 2156
SsiI	CCGC	6 cut(s)	526, 1100, 1790, 1903, 2194, 2213
SspMI	CTAG	3 cut(s)	213, 1298, 1400
SstI	GAGCTC	2 cut(s)	177, 1284
StyD4I	CCNGG	8 cut(s)	154, 562, 852, 867, 2005, 2047, 2048, 2092
StyI	CCWWGG	4 cut(s)	212, 1084, 1482, 1753
TaaI	ACNGT	8 cut(s)	247, 268, 358, 412, 517, 1229, 1345, 2125
TaiI	ACGT	1 cut(s)	2115
TaqI	TCGA	4 cut(s)	763, 1165, 1499, 1686
TatI	WGTACW	2 cut(s)	606, 1287
TauI	GCSGC	1 cut(s)	2196
TfiI	GAWTC	9 cut(s)	129, 230, 313, 755, 776, 1349, 1391, 1621, 1749
Tru1I	TTAA	2 cut(s)	450, 1329
Tru9I	TTAA	2 cut(s)	450, 1329
TscAI	CASTG	2 cut(s)	141, 528
TseFI	GTSAC	1 cut(s)	1293
TseI	GCWGC	4 cut(s)	195, 350, 1097, 1670
Tsp45I	GTSAC	1 cut(s)	1293
TspDTI	ATGAA	6 cut(s)	491, 854, 983, 1587, 1596, 1917
TspGWI	ACGGA	2 cut(s)	626, 1931
TspMI	CCCGGG	1 cut(s)	2048
TspRI	CASTG	2 cut(s)	141, 528
Van91I	CCANNNNNTGG	1 cut(s)	1940
VneI	GTGCAC	1 cut(s)	1974
VpaK11BI	GGWCC	2 cut(s)	865, 2045
XagI	CCTNNNNNAGG	1 cut(s)	998
XapI	RAATTY	6 cut(s)	454, 549, 568, 798, 1154, 1495
XceI	RCATGY	2 cut(s)	1670, 2156
XhoI	CTCGAG	1 cut(s)	762
XmaI	CCCGGG	1 cut(s)	2048
XmaJI	CCTAGG	1 cut(s)	212
XmnI	GAANNNNTTC	1 cut(s)	107
XspI	CTAG	3 cut(s)	213, 1298, 1400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.