Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005806

Physical Location & Seq

Genomic Coordinates Forward (+)

12418783 .. 12424720

Gene Structure 5938 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1135000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2226 bp

ATGGCGACCGGAGCTGTACCAGCTTCCTTTACGGGTCTAAAAGGCAGGGACTCGAGTTTCGGGTTTGCTAAAAGTATGGATTTTGTGAGGGTTTCTGATTTGAAGAGGTTCAAGTCTGGCAGAACAAGAATCTCAGTGATACGAAACTCAAATCCAGGGTCAGATATTGCCGAGCTCAAGCCTGCATCTGAAGGCAGCCCTTTGTTAGGTTTTCCTCGGCAAAAGTATTGTGAATCTGTACATAAAACTGTCAGGAGGAAAACACGCACAGTTATGGTTGGAAATGTGGCTATTGGTAGTGAGCATCCCATAAGGATTCAAACAATGACCACAACTGACACAAAGGATGTCGCTGCTACAGTTGAACAGGTAATGAGAATAGCAGATAAGGGAGCAGATATTGTTCGGATAACAGTTCAGGGGAGGAAAGAAGCAGATGCTTGTTTTGAAATTAAAAATTCCCTCGTGCAGAAGAATTATGATATTCCTTTGGTGGCAGATATTCATTTTGCTCCTACTGTTGCACTGCGAGTCGCTGACTGCTTTGACAAAATTCGTGTCAACCCTGGAAATTTTGCTGATAGACGGGCCCAGTTTGAGAAGCTAGAGTACACGGAAGACGACTATCAGAAAGAACTTGAGCATATTGAGCAGGTTTTTACTCCATTGGTTGAAAAGTGTAAGAAGTATGGAAGGGCAATGCGTATTGGCACAAACCATGGGAGCCTTTCAGATCGTATAATGAGCTATTATGGGGATTCCCCTAGAGGAATGGTTGAATCTGCATTTGAGTTTGCAAGGATTTGCCGGAAGTTGGACTACCATAATTTTGTTTTTTCAATGAAAGCAAGCAACCCAGTTATCATGGTCCAGGCGTATCGTCTGCTTGTAGCTGAAATGTATGTTCAAGGCTGGGATTATCCATTGCACTTGGGAGTTACTGAAGCTGGAGAAGGTGAGGATGGGCGAATGAAATCTGCGATTGGTATTGGAACCCTTCTTCAGGATGGCTTGGGTGATACAATTAGGGTTTCACTTACTGAAGCACCGGAGGAAGAGATAGATCCTTGCCGAAGATTGGCCAACCTTGGTATGAGAGCAGCTGAACTTCAGCAAGGAGTGGCTCCATTTGAAGAGAAGCACCGACATTATTTTGATTTTCAGCGTCGATCTGGTCAACTGCCAATGCAAAAGGAGGGTGAAGAGGTGGATTATAGAGGTGTCCTGCACCGTGATGGATCTGTTCTCATGTCAGTATCCCTTGATCAGTTGAAGACACCGGAGCTCCTGTACAAGTCACTAGCAGCAAAACTCATTCTGGGAATGCCATTTAAGGATCTTGCAACAGTTGACTCGATCTTATTGAGACAACTTCCACCTGTTGACGATGCTGATTCTCGGCTAGCTCTCAAAAGGTTGATAGATGTAAGTATGGGTGTTATCACACCATTGTCAGAACAGCTAACAAAGCCATTGCCCAATGCCATGGTTCTGGTAAATTCAAAGGAGTTATCAACTGGTGCATACAAGCTTTTGCCAGAAGGTACACGCTTGGTGGTCTCACTACGTGGTGATGAACCCTATGAAGTGCTGGATATTCTCAAAGGCATTGATGCTACAATGATTCTCCATGATCTGCCCTTCGGTGAAGATAAAATAAGCCGAGTGCATGCTGCAAGGAGGCTATTCGAGTATCTGGGAGACAATGCTCTAAACTTTCCCGTAATACACCATATTCAGTTTCCAAGTGGAATCCATAGGGATGACTTGGTTATTGGTGCTGGTACCAATGCGGGGGCCCTTTTAGTAGATGGACTTGGAGATGGTCTCCTATTGGAAGCCCCAGACAAGGACTTTGATTTCCTTAGAAACACATCCTTCAATTTACTACAAGGATGTAGAATGCGGAATACAAAGACGGAGTACGTTTCATGCCCATCCTGCGGCCGAACTTTGTTTGATCTTCAAGAAATAAGTGCACAAATACGAGAGAAGACATCACACTTGCCTGGTGTTTCGATTGCAATCATGGGTTGCATTGTAAATGGACCCGGGGAGATGGCTGATGCAGACTTTGGCTATGTTGGCGGTGCTCCTGGAAAGATTGACCTCTATGTTGGGAAGACCGTGGTGAAGCGTGGAATTGAGATGGAGCATGCAACCGATGCCTTGATCCAGCTAATAAAGGATCACGGCCGCTGGGTTGACCCTCCTGCTGAAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006081 GO:0006082 GO:0006090 GO:0006629 GO:0006644 GO:0006720 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009240 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009627 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009862 GO:0009863 GO:0009941 GO:0009987 GO:0010033 GO:0014070 GO:0016491 GO:0016725 GO:0019288 GO:0019637 GO:0019682 GO:0019752 GO:0023052 GO:0031967 GO:0031975 GO:0032787 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046429 GO:0046490 GO:0046677 GO:0048037 GO:0050789 GO:0050794 GO:0050896 GO:0051536 GO:0051539 GO:0051540 GO:0051704 GO:0051707 GO:0051716 GO:0052592 GO:0055114 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0090407 GO:0098542 GO:1901135 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130

Pfam Domains

Protein Families

GcpE

Protein Analysis

742

Amino Acids

82.15

Weight (kDa)

6.11

Isoelectric Point (pI)

35.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GcpE	PF04551	88 - 357	7.9e-90	GcpE TIM-barrel domain
GcpE_C	PF26540	641 - 729	3.3e-24	GcpE protein C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005806)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60600 AT5G60600 AT5G60600 AT5G60600 AT5G60600
fragaria_vesca	FvH4_3g32460 FvH4_3g32460 FvH4_3g32460
malus_domestica	MD03G1117300.v1.1 MD11G1135000.v1.1
prunus_persica	Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1
pyrus_communis	pycom11g11210
rosa_chinensis	RchiOBHm_Chr5g0059471
rosa_laevigata	RLG00000035288
rosa_multiflora	Rmu_sc0000375.1_g000001 Rmu_sc0001977.1_g000002
rosa_roxburghii	Rroxscaffold_1G00021070
rosa_rugosa	Rorug05G0322800 Rorug05G0322900 Rorug05G0322900
rosa_samantha	Rh5BG398900 Rh5DG413800
rosa_wichuraiana	Rw5G036470 Rw5G036510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	643
Acc65I	GGTACC	1 cut(s)	1784
AccB1I	GGYRCC	1 cut(s)	1784
AciI	CCGC	5 cut(s)	1793, 1906, 1944, 2088, 2197
AclWI	GGATC	5 cut(s)	1058, 1246, 1344, 2167, 2196
AcoI	YGGCCR	3 cut(s)	1080, 1945, 2194
AcsI	RAATTY	4 cut(s)	457, 552, 571, 1498
AcuI	CTGAAG	5 cut(s)	210, 963, 986, 1062, 1094
AdeI	CACNNNGTG	1 cut(s)	1568
AfaI	GTAC	7 cut(s)	18, 240, 611, 1292, 1546, 1786, 1925
AfiI	CCNNNNNNNGG	7 cut(s)	206, 814, 1003, 1078, 1794, 1849, 1943
AjnI	CCWGG	5 cut(s)	154, 565, 870, 2008, 2095
Alw21I	GWGCWC	4 cut(s)	177, 1287, 1981, 2095
Alw26I	GTCTC	4 cut(s)	1360, 1564, 1695, 1832
Alw44I	GTGCAC	1 cut(s)	1977
AlwI	GGATC	5 cut(s)	1058, 1246, 1344, 2167, 2196
Ama87I	CYCGRG	2 cut(s)	52, 2051
AoxI	GGCC	5 cut(s)	588, 1080, 1797, 1945, 2194
ApaI	GGGCCC	2 cut(s)	592, 1801
ApaLI	GTGCAC	1 cut(s)	1977
ApeKI	GCWGC	5 cut(s)	195, 353, 1100, 1304, 1673
ApoI	RAATTY	4 cut(s)	457, 552, 571, 1498
ArsI	GACNNNNNNTTYG	8 cut(s)	41, 73, 143, 175, 543, 575, 1838, 1870
Asp700I	GAANNNNTTC	1 cut(s)	107
Asp718I	GGTACC	1 cut(s)	1784
AspS9I	GGNCC	6 cut(s)	588, 589, 868, 1797, 1798, 2048
AsuC2I	CCSGG	2 cut(s)	2052, 2053
AsuHPI	GGTGA	6 cut(s)	968, 1028, 1211, 1583, 1658, 2143
AsuNHI	GCTAGC	1 cut(s)	1402
AvaI	CYCGRG	2 cut(s)	52, 2051
AvaII	GGWCC	2 cut(s)	868, 2048
BaeGI	GKGCMC	3 cut(s)	592, 1801, 1981
BalI	TGGCCA	1 cut(s)	1082
BanI	GGYRCC	1 cut(s)	1784
BanII	GRGCYC	4 cut(s)	177, 592, 1287, 1801
BarI	GAAGNNNNNNTAC	4 cut(s)	593, 625, 803, 835
BauI	CACGAG	1 cut(s)	464
BbsI	GAAGAC	4 cut(s)	624, 1280, 2000, 2129
Bbv12I	GWGCWC	4 cut(s)	177, 1287, 1981, 2095
BbvI	GCAGC	5 cut(s)	207, 340, 1112, 1316, 1660
BccI	CCATC	8 cut(s)	956, 1001, 1229, 1805, 1817, 1945, 2053, 2143
BceAI	ACGGC	1 cut(s)	2209
BciT130I	CCWGG	5 cut(s)	156, 567, 872, 2010, 2097
BciVI	GTATCC	1 cut(s)	1267
BclI	TGATCA	1 cut(s)	1264
BcnI	CCSGG	2 cut(s)	2052, 2053
BcoDI	GTCTC	4 cut(s)	1360, 1564, 1695, 1832
BfaI	CTAG	4 cut(s)	605, 765, 1301, 1403
BfmI	CTRYAG	1 cut(s)	357
BfuAI	ACCTGC	1 cut(s)	643
BfuI	GTATCC	1 cut(s)	1267
BisI	GCNGC	7 cut(s)	196, 354, 1101, 1305, 1674, 1945, 2197
BlsI	GCNGC	7 cut(s)	197, 355, 1102, 1306, 1675, 1946, 2198
Bme1390I	CCNGG	7 cut(s)	156, 567, 872, 2010, 2052, 2053, 2097
Bme18I	GGWCC	2 cut(s)	868, 2048
BmeT110I	CYCGRG	2 cut(s)	52, 2051
BmgT120I	GGNCC	6 cut(s)	588, 589, 868, 1797, 1798, 2048
BmiI	GGNNCC	8 cut(s)	590, 725, 994, 1125, 1786, 1798, 1799, 2050
BmrFI	CCNGG	7 cut(s)	156, 567, 872, 2010, 2052, 2053, 2097
BmrI	ACTGGG	2 cut(s)	586, 851
BmsI	GCATC	7 cut(s)	194, 313, 427, 1378, 1603, 2056, 2155
BmtI	GCTAGC	1 cut(s)	1406
BmuI	ACTGGG	2 cut(s)	586, 851
BpiI	GAAGAC	4 cut(s)	624, 1280, 2000, 2129
BplI	GAGNNNNNCTC	2 cut(s)	1812, 1844
BpmI	CTGGAG	1 cut(s)	969
BpuEI	CTTGAG	2 cut(s)	161, 659
BpuMI	CCSGG	2 cut(s)	2052, 2053
BsaAI	YACGTR	1 cut(s)	1568
BsaBI	GATNNNNATC	1 cut(s)	2024
BsaI	GGTCTC	2 cut(s)	1564, 1832
BsaJI	CCNNGG	9 cut(s)	155, 215, 565, 718, 1087, 1485, 2051, 2052, 2127
BsaWI	WCCGGW	3 cut(s)	8, 1048, 1279
BsaXI	ACNNNNNCTCC	4 cut(s)	1269, 1274, 1299, 1304
Bsc4I	CCNNNNNNNGG	7 cut(s)	206, 814, 1003, 1078, 1794, 1849, 1943
Bse1I	ACTGG	3 cut(s)	592, 857, 1522
Bse3DI	GCAATG	3 cut(s)	705, 923, 1472
Bse8I	GATNNNNATC	1 cut(s)	2024
BseBI	CCWGG	5 cut(s)	156, 567, 872, 2010, 2097
BseDI	CCNNGG	9 cut(s)	155, 215, 565, 718, 1087, 1485, 2051, 2052, 2127
BseGI	GGATG	8 cut(s)	304, 352, 967, 1012, 1768, 1874, 1901, 1937
BseJI	GATNNNNATC	1 cut(s)	2024
BseLI	CCNNNNNNNGG	7 cut(s)	206, 814, 1003, 1078, 1794, 1849, 1943
BseMI	GCAATG	3 cut(s)	705, 923, 1472
BseMII	CTCAG	1 cut(s)	147
BseNI	ACTGG	3 cut(s)	592, 857, 1522
BseSI	GKGCMC	3 cut(s)	592, 1801, 1981
BseX3I	CGGCCG	2 cut(s)	1945, 2194
BseXI	GCAGC	5 cut(s)	207, 340, 1112, 1316, 1660
BseYI	CCCAGC	2 cut(s)	912, 2199
BsgI	GTGCAG	2 cut(s)	488, 1211
Bsh1285I	CGRYCG	3 cut(s)	9, 1948, 2197
BshFI	GGCC	5 cut(s)	590, 1082, 1799, 1947, 2196
BshNI	GGYRCC	1 cut(s)	1784
BsiEI	CGRYCG	3 cut(s)	9, 1948, 2197
BsiHKAI	GWGCWC	4 cut(s)	177, 1287, 1981, 2095
BsiHKCI	CYCGRG	2 cut(s)	52, 2051
BsiSI	CCGG	5 cut(s)	9, 808, 1049, 1280, 2052
BslFI	GGGAC	1 cut(s)	62
BslI	CCNNNNNNNGG	7 cut(s)	206, 814, 1003, 1078, 1794, 1849, 1943
BsmAI	GTCTC	4 cut(s)	1360, 1564, 1695, 1832
BsmFI	GGGAC	1 cut(s)	62
BsmI	GAATGC	2 cut(s)	1329, 1908
BsnI	GGCC	5 cut(s)	590, 1082, 1799, 1947, 2196
Bso31I	GGTCTC	2 cut(s)	1564, 1832
BsoBI	CYCGRG	2 cut(s)	52, 2051
Bsp120I	GGGCCC	2 cut(s)	588, 1797
Bsp1286I	GDGCHC	6 cut(s)	177, 592, 1287, 1801, 1981, 2095
Bsp1407I	TGTACA	2 cut(s)	238, 1290
Bsp19I	CCATGG	2 cut(s)	718, 1485
BspACI	CCGC	5 cut(s)	1793, 1906, 1944, 2088, 2197
BspANI	GGCC	5 cut(s)	590, 1082, 1799, 1947, 2196
BspCNI	CTCAG	1 cut(s)	146
BspLI	GGNNCC	8 cut(s)	590, 725, 994, 1125, 1786, 1798, 1799, 2050
BspMI	ACCTGC	1 cut(s)	643
BspOI	GCTAGC	1 cut(s)	1406
BspPI	GGATC	5 cut(s)	1058, 1246, 1344, 2167, 2196
BspT107I	GGYRCC	1 cut(s)	1784
BspTNI	GGTCTC	2 cut(s)	1564, 1832
BsrDI	GCAATG	3 cut(s)	705, 923, 1472
BsrGI	TGTACA	2 cut(s)	238, 1290
BsrI	ACTGG	3 cut(s)	592, 857, 1522
BssECI	CCNNGG	9 cut(s)	155, 215, 565, 718, 1087, 1485, 2051, 2052, 2127
BssSI	CACGAG	1 cut(s)	464
BssT1I	CCWWGG	3 cut(s)	718, 1087, 1485
Bst2BI	CACGAG	1 cut(s)	464
Bst2UI	CCWGG	5 cut(s)	156, 567, 872, 2010, 2097
Bst4CI	ACNGT	8 cut(s)	250, 271, 361, 415, 520, 1232, 1348, 2128
Bst6I	CTCTTC	5 cut(s)	98, 1050, 1128, 1197, 2214
BstAPI	GCANNNNNTGC	1 cut(s)	2165
BstAUI	TGTACA	2 cut(s)	238, 1290
BstBAI	YACGTR	1 cut(s)	1568
BstC8I	GCNNGC	5 cut(s)	183, 850, 1404, 1671, 2157
BstDEI	CTNAG	2 cut(s)	133, 1865
BstDSI	CCRYGG	3 cut(s)	718, 1485, 2127
BstENI	CCTNNNNNAGG	2 cut(s)	204, 1001
BstF5I	GGATG	8 cut(s)	304, 352, 967, 1012, 1768, 1874, 1901, 1937
BstMAI	GTCTC	4 cut(s)	1360, 1564, 1695, 1832
BstMCI	CGRYCG	3 cut(s)	9, 1948, 2197
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 20, 649, 1468, 1941, 2085, 2165
BstNI	CCWGG	5 cut(s)	156, 567, 872, 2010, 2097
BstNSI	RCATGY	2 cut(s)	1673, 2159
BstSCI	CCNGG	7 cut(s)	154, 565, 870, 2008, 2050, 2051, 2095
BstSFI	CTRYAG	1 cut(s)	357
BstSLI	GKGCMC	3 cut(s)	592, 1801, 1981
BstV1I	GCAGC	5 cut(s)	207, 340, 1112, 1316, 1660
BstV2I	GAAGAC	4 cut(s)	624, 1280, 2000, 2129
BstX2I	RGATCY	3 cut(s)	1063, 1238, 1336
BstXI	CCANNNNNNTGG	2 cut(s)	1486, 1492
BstYI	RGATCY	3 cut(s)	1063, 1238, 1336
BstZI	CGGCCG	2 cut(s)	1945, 2194
BsuI	GTATCC	1 cut(s)	1267
BsuRI	GGCC	5 cut(s)	590, 1082, 1799, 1947, 2196
BtgI	CCRYGG	3 cut(s)	718, 1485, 2127
BtsCI	GGATG	8 cut(s)	304, 352, 967, 1012, 1768, 1874, 1901, 1937
BtsI	GCAGTG	1 cut(s)	524
BtsIMutI	CAGTG	2 cut(s)	141, 524
BveI	ACCTGC	1 cut(s)	643
Cac8I	GCNNGC	5 cut(s)	183, 850, 1404, 1671, 2157
Cfr13I	GGNCC	6 cut(s)	588, 589, 868, 1797, 1798, 2048
Cfr9I	CCCGGG	1 cut(s)	2051
CseI	GACGC	1 cut(s)	1154
Csp6I	GTAC	7 cut(s)	17, 239, 610, 1291, 1545, 1785, 1924
CviAII	CATG	9 cut(s)	719, 865, 1249, 1486, 1631, 1670, 1932, 2029, 2156
CviQI	GTAC	7 cut(s)	17, 239, 610, 1291, 1545, 1785, 1924
DdeI	CTNAG	2 cut(s)	133, 1865
DraIII	CACNNNGTG	1 cut(s)	1568
EaeI	YGGCCR	3 cut(s)	1080, 1945, 2194
EagI	CGGCCG	2 cut(s)	1945, 2194
Eam1104I	CTCTTC	5 cut(s)	98, 1050, 1128, 1197, 2214
EarI	CTCTTC	5 cut(s)	98, 1050, 1128, 1197, 2214
Ecl136II	GAGCTC	2 cut(s)	175, 1285
EclXI	CGGCCG	2 cut(s)	1945, 2194
Eco130I	CCWWGG	3 cut(s)	718, 1087, 1485
Eco24I	GRGCYC	4 cut(s)	177, 592, 1287, 1801
Eco31I	GGTCTC	2 cut(s)	1564, 1832
Eco47I	GGWCC	2 cut(s)	868, 2048
Eco52I	CGGCCG	2 cut(s)	1945, 2194
Eco53kI	GAGCTC	2 cut(s)	175, 1285
Eco57I	CTGAAG	5 cut(s)	210, 963, 986, 1062, 1094
Eco88I	CYCGRG	2 cut(s)	52, 2051
EcoICRI	GAGCTC	2 cut(s)	175, 1285
EcoNI	CCTNNNNNAGG	2 cut(s)	204, 1001
EcoO109I	RGGNCCY	2 cut(s)	1797, 1798
EcoRII	CCWGG	5 cut(s)	154, 565, 870, 2008, 2095
EcoT14I	CCWWGG	3 cut(s)	718, 1087, 1485
EcoT38I	GRGCYC	4 cut(s)	177, 592, 1287, 1801
ErhI	CCWWGG	3 cut(s)	718, 1087, 1485
FaeI	CATG	9 cut(s)	722, 868, 1252, 1489, 1634, 1673, 1935, 2032, 2159
FaqI	GGGAC	1 cut(s)	62
FatI	CATG	9 cut(s)	718, 864, 1248, 1485, 1630, 1669, 1931, 2028, 2155
FauI	CCCGC	1 cut(s)	1786
FbaI	TGATCA	1 cut(s)	1264
Fnu4HI	GCNGC	7 cut(s)	196, 354, 1101, 1305, 1674, 1945, 2197
FokI	GGATG	8 cut(s)	291, 359, 974, 1019, 1775, 1861, 1908, 1924
FriOI	GRGCYC	4 cut(s)	177, 592, 1287, 1801
Fsp4HI	GCNGC	7 cut(s)	196, 354, 1101, 1305, 1674, 1945, 2197
FspBI	CTAG	4 cut(s)	605, 765, 1301, 1403
GluI	GCNGC	7 cut(s)	196, 354, 1101, 1305, 1674, 1945, 2197
GsaI	CCCAGC	2 cut(s)	916, 2203
GsuI	CTGGAG	1 cut(s)	969
HaeIII	GGCC	5 cut(s)	590, 1082, 1799, 1947, 2196
HapII	CCGG	5 cut(s)	9, 808, 1049, 1280, 2052
HgaI	GACGC	1 cut(s)	1154
Hin1II	CATG	9 cut(s)	722, 868, 1252, 1489, 1634, 1673, 1935, 2032, 2159
HincII	GTYRAC	5 cut(s)	562, 1178, 1351, 1384, 2206
HindII	GTYRAC	5 cut(s)	562, 1178, 1351, 1384, 2206
HindIII	AAGCTT	1 cut(s)	1529
HpaII	CCGG	5 cut(s)	9, 808, 1049, 1280, 2052
HphI	GGTGA	6 cut(s)	968, 1028, 1211, 1583, 1658, 2143
Hpy166II	GTNNAC	8 cut(s)	562, 612, 1178, 1351, 1384, 1547, 1979, 2206
Hpy188I	TCNGA	7 cut(s)	97, 163, 190, 408, 630, 733, 1456
Hpy188III	TCNNGA	3 cut(s)	253, 1004, 1967
Hpy8I	GTNNAC	8 cut(s)	562, 612, 1178, 1351, 1384, 1547, 1979, 2206
Hpy99I	CGWCG	1 cut(s)	1170
HpyAV	CCTTC	7 cut(s)	185, 687, 947, 1007, 1535, 1651, 1888
HpyCH4III	ACNGT	8 cut(s)	250, 271, 361, 415, 520, 1232, 1348, 2128
HpyCH4IV	ACGT	2 cut(s)	1567, 1926
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 20, 649, 1468, 1941, 2085, 2165
HpyF3I	CTNAG	2 cut(s)	133, 1865
HpySE526I	ACGT	2 cut(s)	1567, 1926
Hsp92II	CATG	9 cut(s)	722, 868, 1252, 1489, 1634, 1673, 1935, 2032, 2159
KpnI	GGTACC	1 cut(s)	1788
Ksp22I	TGATCA	1 cut(s)	1264
LmnI	GCTCC	9 cut(s)	11, 392, 517, 723, 1129, 1282, 1290, 2098, 2152
Lsp1109I	GCAGC	5 cut(s)	207, 340, 1112, 1316, 1660
LweI	GCATC	7 cut(s)	194, 313, 427, 1378, 1603, 2056, 2155
MaeI	CTAG	4 cut(s)	605, 765, 1301, 1403
MaeII	ACGT	2 cut(s)	1567, 1926
MaeIII	GTNAC	2 cut(s)	937, 1296
MflI	RGATCY	3 cut(s)	1063, 1238, 1336
MhlI	GDGCHC	6 cut(s)	177, 592, 1287, 1801, 1981, 2095
MlsI	TGGCCA	1 cut(s)	1082
MluNI	TGGCCA	1 cut(s)	1082
MlyI	GAGTC	3 cut(s)	44, 540, 1346
MmeI	TCCRAC	2 cut(s)	259, 795
Mox20I	TGGCCA	1 cut(s)	1082
MroXI	GAANNNNTTC	1 cut(s)	107
MscI	TGGCCA	1 cut(s)	1082
MseI	TTAA	2 cut(s)	453, 1332
MslI	CAYNNNNRTG	3 cut(s)	272, 1233, 1761
Msp20I	TGGCCA	1 cut(s)	1082
MspA1I	CMGCKG	2 cut(s)	1103, 2199
MspI	CCGG	5 cut(s)	9, 808, 1049, 1280, 2052
MspR9I	CCNGG	7 cut(s)	156, 567, 872, 2010, 2052, 2053, 2097
Mva1269I	GAATGC	2 cut(s)	1329, 1908
MvaI	CCWGG	5 cut(s)	156, 567, 872, 2010, 2097
MwoI	GCNNNNNNNGC	7 cut(s)	11, 20, 649, 1468, 1941, 2085, 2165
NciI	CCSGG	2 cut(s)	2052, 2053
NcoI	CCATGG	2 cut(s)	718, 1485
NheI	GCTAGC	1 cut(s)	1402
NlaIII	CATG	9 cut(s)	722, 868, 1252, 1489, 1634, 1673, 1935, 2032, 2159
NlaIV	GGNNCC	8 cut(s)	590, 725, 994, 1125, 1786, 1798, 1799, 2050
NmeAIII	GCCGAG	4 cut(s)	196, 196, 1378, 1688
NmuCI	GTSAC	1 cut(s)	1296
NspI	RCATGY	2 cut(s)	1673, 2159
PaeI	GCATGC	2 cut(s)	1673, 2159
PaeR7I	CTCGAG	1 cut(s)	52
PctI	GAATGC	2 cut(s)	1329, 1908
PdmI	GAANNNNTTC	1 cut(s)	107
PfeI	GAWTC	8 cut(s)	129, 233, 316, 758, 779, 1394, 1624, 1752
PfoI	TCCNGGA	1 cut(s)	2095
PkrI	GCNGC	7 cut(s)	197, 355, 1102, 1306, 1675, 1946, 2198
PleI	GAGTC	3 cut(s)	44, 539, 1346
PpsI	GAGTC	3 cut(s)	44, 539, 1346
Ppu21I	YACGTR	1 cut(s)	1568
Psp124BI	GAGCTC	2 cut(s)	177, 1287
Psp6I	CCWGG	5 cut(s)	154, 565, 870, 2008, 2095
PspFI	CCCAGC	2 cut(s)	912, 2199
PspGI	CCWGG	5 cut(s)	154, 565, 870, 2008, 2095
PspN4I	GGNNCC	8 cut(s)	590, 725, 994, 1125, 1786, 1798, 1799, 2050
PspOMI	GGGCCC	2 cut(s)	588, 1797
PspPI	GGNCC	6 cut(s)	588, 589, 868, 1797, 1798, 2048
PspXI	VCTCGAGB	1 cut(s)	52
PsuI	RGATCY	3 cut(s)	1063, 1238, 1336
PvuII	CAGCTG	1 cut(s)	1103
RsaI	GTAC	7 cut(s)	18, 240, 611, 1292, 1546, 1786, 1925
RsaNI	GTAC	7 cut(s)	17, 239, 610, 1291, 1545, 1785, 1924
RseI	CAYNNNNRTG	3 cut(s)	272, 1233, 1761
SacI	GAGCTC	2 cut(s)	177, 1287
SaqAI	TTAA	2 cut(s)	453, 1332
SatI	GCNGC	7 cut(s)	196, 354, 1101, 1305, 1674, 1945, 2197
Sau96I	GGNCC	6 cut(s)	588, 589, 868, 1797, 1798, 2048
SchI	GAGTC	3 cut(s)	44, 540, 1346
ScrFI	CCNGG	7 cut(s)	156, 567, 872, 2010, 2052, 2053, 2097
SduI	GDGCHC	6 cut(s)	177, 592, 1287, 1801, 1981, 2095
SfaNI	GCATC	7 cut(s)	194, 313, 427, 1378, 1603, 2056, 2155
SfcI	CTRYAG	1 cut(s)	357
Sfr274I	CTCGAG	1 cut(s)	52
SinI	GGWCC	2 cut(s)	868, 2048
SlaI	CTCGAG	1 cut(s)	52
SmaI	CCCGGG	1 cut(s)	2053
SmiMI	CAYNNNNRTG	3 cut(s)	272, 1233, 1761
SmlI	CTYRAG	3 cut(s)	52, 176, 638
SmoI	CTYRAG	3 cut(s)	52, 176, 638
SphI	GCATGC	2 cut(s)	1673, 2159
SsiI	CCGC	5 cut(s)	1793, 1906, 1944, 2088, 2197
SspMI	CTAG	4 cut(s)	605, 765, 1301, 1403
SstI	GAGCTC	2 cut(s)	177, 1287
StyD4I	CCNGG	7 cut(s)	154, 565, 870, 2008, 2050, 2051, 2095
StyI	CCWWGG	3 cut(s)	718, 1087, 1485
TaaI	ACNGT	8 cut(s)	250, 271, 361, 415, 520, 1232, 1348, 2128
TaiI	ACGT	2 cut(s)	1570, 1929
TaqI	TCGA	5 cut(s)	53, 1168, 1355, 1689, 2018
TatI	WGTACW	3 cut(s)	238, 609, 1290
TauI	GCSGC	2 cut(s)	1947, 2199
TfiI	GAWTC	8 cut(s)	129, 233, 316, 758, 779, 1394, 1624, 1752
Tru1I	TTAA	2 cut(s)	453, 1332
Tru9I	TTAA	2 cut(s)	453, 1332
TscAI	CASTG	2 cut(s)	141, 531
TseFI	GTSAC	1 cut(s)	1296
TseI	GCWGC	5 cut(s)	195, 353, 1100, 1304, 1673
Tsp45I	GTSAC	1 cut(s)	1296
TspDTI	ATGAA	6 cut(s)	494, 857, 986, 1590, 1599, 1920
TspGWI	ACGGA	2 cut(s)	629, 1934
TspMI	CCCGGG	1 cut(s)	2051
TspRI	CASTG	2 cut(s)	141, 531
VneI	GTGCAC	1 cut(s)	1977
VpaK11BI	GGWCC	2 cut(s)	868, 2048
XagI	CCTNNNNNAGG	2 cut(s)	204, 1001
XapI	RAATTY	4 cut(s)	457, 552, 571, 1498
XceI	RCATGY	2 cut(s)	1673, 2159
XhoI	CTCGAG	1 cut(s)	52
XmaI	CCCGGG	1 cut(s)	2051
XmnI	GAANNNNTTC	1 cut(s)	107
XspI	CTAG	4 cut(s)	605, 765, 1301, 1403

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.