Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005806

Physical Location & Seq

Genomic Coordinates Forward (+)

25767843 .. 25774969

Gene Structure 7127 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00021070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2223 bp

ATGGCTACTGGAGCTGTACCGGCTTCCTTTACGGGCCTAAAAGGCAGGGAGACGAGGTTGGGGTTCACTAAAAGCATGGATTTTGTGAGGATTTCTGATTTGAAGCGGTTTAAGTCTGGCAGAACTAGGATTTCAGTGATAAGGAACTCGAATCCTGGATCGGATATTGCTGAACTCAAGCCTGCATCTGAAGGCAGCCCTTTGTTAGTTCCTAGACAAAAGTATTGTGAATCCATACACAAAACTGTCAGAAGAAAAACAAGGACGGTGATGGTTGGAAATGTGGCTATTGGCAGTGAGCATCCCATAAGGATTCAAACAATGACAACAAGTGACACAAAGGATGTATCTGCTACAGTTGAAGAGGTGATGAGAATAGCAGACAAGGGTGCAGATATTGTTCGGATAACAGTTCAAGGGAAGAAGGAAGCAGATGCTTGTTATGAGATTAAAAATTCCCTGGTGCAGAAAAATTACAATATACCTCTGGTGGCAGATATTCATTTTGCTCCTTCTGTTGCGTTGCGAGTTGCTGAATGCTTTGACAAAATTCGTGTCAACCCTGGAAATTTTGCTGATAGACGCGCACAATTTGAGAAGTTAGAGTACACAGAAGATGACTATGAGAAAGAACTTGAGCACATTGAGCAGGTTTTTACTCCATTGGTTGAAAAATGTAAGAAGTATGGAAGAGCAATGCGTATTGGCACAAACCATGGGAGCCTTTCAGATCGTATAATGAGCTACTATGGAGATTCACCTAGAGGAATGGTAGAATCTGCATTTGAGTTTGCGAGGATCTGCAGGAAGTTGGACTACCATAACTTTCTTTTCTCAATGAAAGCAAGCAATCCGGTTATTATGGTCCAAGCTTATCGTCTTCTTGTGGCTGAAATGTATGTTCAAGGCTGGGATTATCCATTACACTTGGGTGTCACTGAAGCTGGAGAAGGTGAGGATGGACGGATGAAATCTGCAATTGGTATTGGGACCCTTCTTCAGGATGGTTTGGGTGATACAATTAGGGTTTCACTTACTGAAGCACCCGAGGAGGAGATCGATCCTTGCAAAAGATTGGCCAACCTTGGTAAAAGAGCAGCTGATCTCCAGCAAGGAGTGGATCCATTTGAAGAGAAACACCGACATTACTTTGATTTTCAGCGGCGATCTGGTCAACTGCCAGTGCAAAAGGAGGGTGATGAGGTGGATTATAGAGGGGTCCTGCATCGTGATGGCTCAGTTCTGATGTCAGTCTCCCTTGATCAGTTGAAGACACCAGAGCTTCTGTACAAGTCACTTGCAGCAAAACTTGTTGTGGGCATGCCATTTAAGGATCTGGCAACAGTTGACTCGATCTTATTGAGACAACTACCACCTGTTGACGACGATGATTCTCGTCTGGCTCTCAAAAGGTTGATAGATGTAAGTATGGGTGTTATAACGCCATTGTCAGAGCAGCTTACGAAACCATTACCAAATGCCATGGTTCTGCTAAATTCAAAGGAATTATCAACTGGTGCATACAAGCTTTTGCCAGAAGGTACACGCTTGGTTGTCTCGGTCCGTGGTGATGAACCCTATGAAGTTCTGGATCTTCTCAAAGGCCTTGATGTTACAATGCTTCTCCATGACCTGCCATTCAGTGAAGAGAAAATAAGCCGAGTGCATGCAGCAAGAAGGCTTTTTGAGTATCTGGGAGACAATGATCTAAACTTTCCTGTAATACACCATATTCAGTTTCCAAGTGGAATTCATAGGGATGACTTGGTTATTGGTGCTGGTACCAATGCGGGAGCCCTTTTAGTAGATGGGCTTGGAGATGGTCTCCTATTAGAAGCCCCTGACAAGGATTTCGAGTTCATTAGAAACACATCTTTCAATTTACTACAAGGTTGTAGAATGCGGAACACAAAGACGGAGTATGTCTCTTGCCCATCATGTGGAAGAACTTTGTTTGATCTGCAAGAAATAAGTGCACAAATACGAGAGAAGACATCGCACTTGCCTGGTGTTTCAATTGCAATCATGGGTTGCATTGTAAATGGACCTGGGGAGATGGCAGATGCAGACTTTGGGTATGTTGGTGGTGCTCCTGGAAAGATTGATCTTTATGTTGGGAAGACCGTGGTGAAGCGTGGAATAGAGATGGAGCATGCAACTGATGCCTTGATCCAGCTAATAAAAGATCATGATCGCTGGGTAGACCCTCCAGCGGAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006081 GO:0006082 GO:0006090 GO:0006629 GO:0006644 GO:0006720 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009240 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009627 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009862 GO:0009863 GO:0009941 GO:0009987 GO:0010033 GO:0014070 GO:0016491 GO:0016725 GO:0019288 GO:0019637 GO:0019682 GO:0019752 GO:0023052 GO:0031967 GO:0031975 GO:0032787 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046429 GO:0046490 GO:0046677 GO:0048037 GO:0050789 GO:0050794 GO:0050896 GO:0051536 GO:0051539 GO:0051540 GO:0051704 GO:0051707 GO:0051716 GO:0052592 GO:0055114 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0090407 GO:0098542 GO:1901135 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130

Pfam Domains

Protein Families

GcpE

Protein Analysis

740

Amino Acids

82.33

Weight (kDa)

5.92

Isoelectric Point (pI)

35.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GcpE	PF04551	87 - 356	5.9e-89	GcpE TIM-barrel domain
GcpE_C	PF26540	640 - 728	3.3e-24	GcpE protein C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005806)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60600 AT5G60600 AT5G60600 AT5G60600 AT5G60600
fragaria_vesca	FvH4_3g32460 FvH4_3g32460 FvH4_3g32460
malus_domestica	MD03G1117300.v1.1 MD11G1135000.v1.1
prunus_persica	Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1 Prupe.6G101000_v2.0.a1
pyrus_communis	pycom11g11210
rosa_chinensis	RchiOBHm_Chr5g0059471
rosa_laevigata	RLG00000035288
rosa_multiflora	Rmu_sc0000375.1_g000001 Rmu_sc0001977.1_g000002
rosa_roxburghii	Rroxscaffold_1G00021070
rosa_rugosa	Rorug05G0322800 Rorug05G0322900 Rorug05G0322900
rosa_samantha	Rh5BG398900 Rh5DG413800
rosa_wichuraiana	Rw5G036470 Rw5G036510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1439
Acc36I	ACCTGC	2 cut(s)	640, 1641
Acc65I	GGTACC	1 cut(s)	1781
AccB1I	GGYRCC	1 cut(s)	1781
AccB7I	CCANNNNNTGG	1 cut(s)	1940
AccI	GTMKAC	1 cut(s)	2202
AccII	CGCG	1 cut(s)	585
AciI	CCGC	5 cut(s)	106, 1162, 1790, 1903, 2213
AclWI	GGATC	8 cut(s)	166, 806, 1055, 1115, 1128, 1341, 1599, 2164
AcoI	YGGCCR	1 cut(s)	1077
AcsI	RAATTY	5 cut(s)	454, 549, 568, 1495, 1749
AcuI	CTGAAG	4 cut(s)	210, 960, 983, 1059
AfaI	GTAC	5 cut(s)	18, 608, 1289, 1543, 1783
AfiI	CCNNNNNNNGG	4 cut(s)	1000, 1846, 1940, 2212
AjnI	CCWGG	6 cut(s)	154, 459, 562, 2005, 2047, 2092
AleI	CACNNNNGTG	1 cut(s)	930
AloI	GAACNNNNNNTCC	2 cut(s)	1841, 1873
AluBI	AGCT	9 cut(s)	14, 744, 872, 944, 1100, 1282, 1459, 1528, 2176
AluI	AGCT	9 cut(s)	14, 744, 872, 944, 1100, 1282, 1459, 1528, 2176
Alw21I	GWGCWC	3 cut(s)	642, 1978, 2092
Alw26I	GTCTC	7 cut(s)	44, 1258, 1357, 1561, 1692, 1829, 1930
Alw44I	GTGCAC	1 cut(s)	1974
AlwI	GGATC	8 cut(s)	166, 806, 1055, 1115, 1128, 1341, 1599, 2164
Ama87I	CYCGRG	1 cut(s)	1046
AoxI	GGCC	3 cut(s)	34, 1077, 1603
ApaLI	GTGCAC	1 cut(s)	1974
ApeKI	GCWGC	5 cut(s)	195, 1097, 1301, 1456, 1670
ApoI	RAATTY	5 cut(s)	454, 549, 568, 1495, 1749
ArsI	GACNNNNNNTTYG	4 cut(s)	540, 572, 1835, 1867
Asp718I	GGTACC	1 cut(s)	1781
AspLEI	GCGC	1 cut(s)	587
AspS9I	GGNCC	6 cut(s)	34, 865, 990, 1219, 1561, 2045
AsuHPI	GGTGA	8 cut(s)	280, 379, 750, 965, 1025, 1208, 1580, 2140
AvaI	CYCGRG	1 cut(s)	1046
AvaII	GGWCC	5 cut(s)	865, 990, 1219, 1561, 2045
BaeGI	GKGCMC	1 cut(s)	1978
BalI	TGGCCA	1 cut(s)	1079
BamHI	GGATCC	1 cut(s)	1120
BanI	GGYRCC	1 cut(s)	1781
BanII	GRGCYC	1 cut(s)	1798
BarI	GAAGNNNNNNTAC	4 cut(s)	590, 622, 800, 832
BbsI	GAAGAC	4 cut(s)	872, 1277, 1997, 2126
Bbv12I	GWGCWC	3 cut(s)	642, 1978, 2092
BbvI	GCAGC	5 cut(s)	207, 1109, 1313, 1468, 1682
BccI	CCATC	9 cut(s)	265, 953, 998, 1226, 1802, 1814, 1942, 2050, 2140
BciT130I	CCWGG	6 cut(s)	156, 461, 564, 2007, 2049, 2094
BclI	TGATCA	1 cut(s)	1261
BcoDI	GTCTC	7 cut(s)	44, 1258, 1357, 1561, 1692, 1829, 1930
BfaI	CTAG	3 cut(s)	126, 213, 762
BfmI	CTRYAG	2 cut(s)	354, 802
BfuAI	ACCTGC	2 cut(s)	640, 1641
BglI	GCCNNNNNGGC	1 cut(s)	42
BisI	GCNGC	6 cut(s)	196, 1098, 1163, 1302, 1457, 1671
BlsI	GCNGC	6 cut(s)	197, 1099, 1164, 1303, 1458, 1672
Bme1390I	CCNGG	6 cut(s)	156, 461, 564, 2007, 2049, 2094
Bme18I	GGWCC	5 cut(s)	865, 990, 1219, 1561, 2045
BmeT110I	CYCGRG	1 cut(s)	1046
BmgT120I	GGNCC	6 cut(s)	34, 865, 990, 1219, 1561, 2045
BmiI	GGNNCC	7 cut(s)	722, 991, 992, 1122, 1220, 1783, 1795
BmrFI	CCNGG	6 cut(s)	156, 461, 564, 2007, 2049, 2094
BmsI	GCATC	6 cut(s)	194, 310, 424, 1234, 2053, 2152
BpiI	GAAGAC	4 cut(s)	872, 1277, 1997, 2126
BplI	GAGNNNNNCTC	4 cut(s)	1809, 1841, 1910, 1942
BpmI	CTGGAG	4 cut(s)	30, 966, 1091, 2193
BpuEI	CTTGAG	2 cut(s)	161, 656
Bsa29I	ATCGAT	1 cut(s)	1059
BsaBI	GATNNNNATC	1 cut(s)	2190
BsaI	GGTCTC	1 cut(s)	1829
BsaJI	CCNNGG	9 cut(s)	459, 562, 715, 1047, 1084, 1482, 1564, 2048, 2124
BsaWI	WCCGGW	1 cut(s)	853
BsaXI	ACNNNNNCTCC	2 cut(s)	41, 71
Bsc4I	CCNNNNNNNGG	4 cut(s)	1000, 1846, 1940, 2212
Bse118I	RCCGGY	1 cut(s)	19
Bse1I	ACTGG	3 cut(s)	13, 1181, 1519
Bse3DI	GCAATG	1 cut(s)	702
Bse8I	GATNNNNATC	1 cut(s)	2190
BseBI	CCWGG	6 cut(s)	156, 461, 564, 2007, 2049, 2094
BseCI	ATCGAT	1 cut(s)	1059
BseDI	CCNNGG	9 cut(s)	459, 562, 715, 1047, 1084, 1482, 1564, 2048, 2124
BseGI	GGATG	6 cut(s)	301, 349, 964, 972, 1009, 1765
BseJI	GATNNNNATC	1 cut(s)	2190
BseLI	CCNNNNNNNGG	4 cut(s)	1000, 1846, 1940, 2212
BseMI	GCAATG	1 cut(s)	702
BseMII	CTCAG	1 cut(s)	1251
BseNI	ACTGG	3 cut(s)	13, 1181, 1519
BseRI	GAGGAG	2 cut(s)	1064, 1067
BseSI	GKGCMC	1 cut(s)	1978
BseXI	GCAGC	5 cut(s)	207, 1109, 1313, 1468, 1682
BseYI	CCCAGC	2 cut(s)	909, 2196
BsgI	GTGCAG	2 cut(s)	411, 485
Bsh1236I	CGCG	1 cut(s)	585
BshFI	GGCC	3 cut(s)	36, 1079, 1605
BshNI	GGYRCC	1 cut(s)	1781
BshVI	ATCGAT	1 cut(s)	1059
BsiHKAI	GWGCWC	3 cut(s)	642, 1978, 2092
BsiHKCI	CYCGRG	1 cut(s)	1046
BsiSI	CCGG	2 cut(s)	20, 854
BslFI	GGGAC	1 cut(s)	1003
BslI	CCNNNNNNNGG	4 cut(s)	1000, 1846, 1940, 2212
BsmAI	GTCTC	7 cut(s)	44, 1258, 1357, 1561, 1692, 1829, 1930
BsmBI	CGTCTC	1 cut(s)	44
BsmFI	GGGAC	1 cut(s)	1003
BsmI	GAATGC	2 cut(s)	542, 1905
BsnI	GGCC	3 cut(s)	36, 1079, 1605
Bso31I	GGTCTC	1 cut(s)	1829
BsoBI	CYCGRG	1 cut(s)	1046
Bsp1286I	GDGCHC	4 cut(s)	642, 1798, 1978, 2092
Bsp1407I	TGTACA	1 cut(s)	1287
Bsp19I	CCATGG	2 cut(s)	715, 1482
BspACI	CCGC	5 cut(s)	106, 1162, 1790, 1903, 2213
BspANI	GGCC	3 cut(s)	36, 1079, 1605
BspCNI	CTCAG	1 cut(s)	1250
BspDI	ATCGAT	1 cut(s)	1059
BspFNI	CGCG	1 cut(s)	585
BspHI	TCATGA	1 cut(s)	2188
BspLI	GGNNCC	7 cut(s)	722, 991, 992, 1122, 1220, 1783, 1795
BspMAI	CTGCAG	1 cut(s)	806
BspMI	ACCTGC	2 cut(s)	640, 1641
BspPI	GGATC	8 cut(s)	166, 806, 1055, 1115, 1128, 1341, 1599, 2164
BspQI	GCTCTTC	1 cut(s)	685
BspT107I	GGYRCC	1 cut(s)	1781
BspTNI	GGTCTC	1 cut(s)	1829
BsrDI	GCAATG	1 cut(s)	702
BsrFI	RCCGGY	1 cut(s)	19
BsrGI	TGTACA	1 cut(s)	1287
BsrI	ACTGG	3 cut(s)	13, 1181, 1519
BssAI	RCCGGY	1 cut(s)	19
BssECI	CCNNGG	9 cut(s)	459, 562, 715, 1047, 1084, 1482, 1564, 2048, 2124
BssT1I	CCWWGG	3 cut(s)	715, 1084, 1482
Bst2UI	CCWGG	6 cut(s)	156, 461, 564, 2007, 2049, 2094
Bst4CI	ACNGT	6 cut(s)	247, 268, 358, 412, 1345, 2125
Bst6I	CTCTTC	5 cut(s)	357, 685, 1125, 1641, 2211
BstAPI	GCANNNNNTGC	1 cut(s)	2162
BstAUI	TGTACA	1 cut(s)	1287
BstC8I	GCNNGC	5 cut(s)	183, 847, 1322, 1668, 2154
BstDEI	CTNAG	1 cut(s)	1237
BstDSI	CCRYGG	4 cut(s)	715, 1482, 1564, 2124
BstENI	CCTNNNNNAGG	1 cut(s)	998
BstF5I	GGATG	6 cut(s)	301, 349, 964, 972, 1009, 1765
BstFNI	CGCG	1 cut(s)	585
BstHHI	GCGC	1 cut(s)	587
BstMAI	GTCTC	7 cut(s)	44, 1258, 1357, 1561, 1692, 1829, 1930
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 20, 42, 646, 2162
BstNI	CCWGG	6 cut(s)	156, 461, 564, 2007, 2049, 2094
BstNSI	RCATGY	3 cut(s)	1324, 1670, 2156
BstSCI	CCNGG	6 cut(s)	154, 459, 562, 2005, 2047, 2092
BstSFI	CTRYAG	2 cut(s)	354, 802
BstSLI	GKGCMC	1 cut(s)	1978
BstUI	CGCG	1 cut(s)	585
BstV1I	GCAGC	5 cut(s)	207, 1109, 1313, 1468, 1682
BstV2I	GAAGAC	4 cut(s)	872, 1277, 1997, 2126
BstX2I	RGATCY	4 cut(s)	798, 1120, 1333, 1591
BstYI	RGATCY	4 cut(s)	798, 1120, 1333, 1591
Bsu15I	ATCGAT	1 cut(s)	1059
BsuRI	GGCC	3 cut(s)	36, 1079, 1605
BsuTUI	ATCGAT	1 cut(s)	1059
BtgI	CCRYGG	4 cut(s)	715, 1482, 1564, 2124
BtgZI	GCGATG	1 cut(s)	1980
BtsCI	GGATG	6 cut(s)	301, 349, 964, 972, 1009, 1765
BtsI	GCAGTG	1 cut(s)	301
BtsIMutI	CAGTG	5 cut(s)	141, 301, 936, 1188, 1648
BveI	ACCTGC	2 cut(s)	640, 1641
Cac8I	GCNNGC	5 cut(s)	183, 847, 1322, 1668, 2154
CciI	TCATGA	1 cut(s)	2188
CfoI	GCGC	1 cut(s)	587
Cfr10I	RCCGGY	1 cut(s)	19
Cfr13I	GGNCC	6 cut(s)	34, 865, 990, 1219, 1561, 2045
ClaI	ATCGAT	1 cut(s)	1059
CpoI	CGGWCCG	1 cut(s)	1561
CseI	GACGC	1 cut(s)	591
Csp6I	GTAC	5 cut(s)	17, 607, 1288, 1542, 1782
CspI	CGGWCCG	1 cut(s)	1561
CviQI	GTAC	5 cut(s)	17, 607, 1288, 1542, 1782
DdeI	CTNAG	1 cut(s)	1237
EaeI	YGGCCR	1 cut(s)	1077
Eam1104I	CTCTTC	5 cut(s)	357, 685, 1125, 1641, 2211
EarI	CTCTTC	5 cut(s)	357, 685, 1125, 1641, 2211
Eco130I	CCWWGG	3 cut(s)	715, 1084, 1482
Eco147I	AGGCCT	1 cut(s)	1605
Eco24I	GRGCYC	1 cut(s)	1798
Eco31I	GGTCTC	1 cut(s)	1829
Eco47I	GGWCC	5 cut(s)	865, 990, 1219, 1561, 2045
Eco57I	CTGAAG	4 cut(s)	210, 960, 983, 1059
Eco88I	CYCGRG	1 cut(s)	1046
EcoNI	CCTNNNNNAGG	1 cut(s)	998
EcoO109I	RGGNCCY	2 cut(s)	990, 1219
EcoRI	GAATTC	1 cut(s)	1749
EcoRII	CCWGG	6 cut(s)	154, 459, 562, 2005, 2047, 2092
EcoT14I	CCWWGG	3 cut(s)	715, 1084, 1482
EcoT38I	GRGCYC	1 cut(s)	1798
ErhI	CCWWGG	3 cut(s)	715, 1084, 1482
Esp3I	CGTCTC	1 cut(s)	44
FaqI	GGGAC	1 cut(s)	1003
FauI	CCCGC	1 cut(s)	1783
FbaI	TGATCA	1 cut(s)	1261
FblI	GTMKAC	1 cut(s)	2202
Fnu4HI	GCNGC	6 cut(s)	196, 1098, 1163, 1302, 1457, 1671
FokI	GGATG	6 cut(s)	288, 356, 971, 979, 1016, 1772
FriOI	GRGCYC	1 cut(s)	1798
Fsp4HI	GCNGC	6 cut(s)	196, 1098, 1163, 1302, 1457, 1671
FspBI	CTAG	3 cut(s)	126, 213, 762
GlaI	GCGC	1 cut(s)	586
GluI	GCNGC	6 cut(s)	196, 1098, 1163, 1302, 1457, 1671
GsaI	CCCAGC	2 cut(s)	913, 2200
GsuI	CTGGAG	4 cut(s)	30, 966, 1091, 2193
HaeIII	GGCC	3 cut(s)	36, 1079, 1605
HapII	CCGG	2 cut(s)	20, 854
HgaI	GACGC	1 cut(s)	591
HhaI	GCGC	1 cut(s)	587
Hin6I	GCGC	1 cut(s)	585
HinP1I	GCGC	1 cut(s)	585
HincII	GTYRAC	4 cut(s)	559, 1175, 1348, 1381
HindII	GTYRAC	4 cut(s)	559, 1175, 1348, 1381
HindIII	AAGCTT	2 cut(s)	870, 1526
HinfI	GANTC	7 cut(s)	151, 230, 313, 755, 776, 1349, 1391
HpaII	CCGG	2 cut(s)	20, 854
HphI	GGTGA	8 cut(s)	280, 379, 750, 965, 1025, 1208, 1580, 2140
Hpy166II	GTNNAC	9 cut(s)	66, 559, 609, 1175, 1348, 1381, 1544, 1976, 2203
Hpy188I	TCNGA	8 cut(s)	97, 163, 190, 251, 405, 730, 1245, 1453
Hpy188III	TCNNGA	4 cut(s)	1001, 1229, 1589, 2189
Hpy8I	GTNNAC	9 cut(s)	66, 559, 609, 1175, 1348, 1381, 1544, 1976, 2203
Hpy99I	CGWCG	1 cut(s)	1388
HpyAV	CCTTC	7 cut(s)	185, 418, 522, 944, 1004, 1532, 1671
HpyCH4III	ACNGT	6 cut(s)	247, 268, 358, 412, 1345, 2125
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 20, 42, 646, 2162
HpyF3I	CTNAG	1 cut(s)	1237
HspAI	GCGC	1 cut(s)	585
KflI	GGGWCCC	1 cut(s)	990
KpnI	GGTACC	1 cut(s)	1785
Ksp22I	TGATCA	1 cut(s)	1261
LguI	GCTCTTC	1 cut(s)	685
LmnI	GCTCC	6 cut(s)	11, 514, 720, 1793, 2095, 2149
Lsp1109I	GCAGC	5 cut(s)	207, 1109, 1313, 1468, 1682
LweI	GCATC	6 cut(s)	194, 310, 424, 1234, 2053, 2152
MaeI	CTAG	3 cut(s)	126, 213, 762
MaeIII	GTNAC	4 cut(s)	332, 934, 1293, 1612
MfeI	CAATTG	2 cut(s)	978, 2016
MflI	RGATCY	4 cut(s)	798, 1120, 1333, 1591
MhlI	GDGCHC	4 cut(s)	642, 1798, 1978, 2092
MlsI	TGGCCA	1 cut(s)	1079
MluNI	TGGCCA	1 cut(s)	1079
MlyI	GAGTC	1 cut(s)	1343
MmeI	TCCRAC	2 cut(s)	256, 792
Mox20I	TGGCCA	1 cut(s)	1079
MscI	TGGCCA	1 cut(s)	1079
MseI	TTAA	3 cut(s)	111, 450, 1329
MslI	CAYNNNNRTG	3 cut(s)	930, 1230, 1758
Msp20I	TGGCCA	1 cut(s)	1079
MspA1I	CMGCKG	3 cut(s)	1100, 1162, 2213
MspI	CCGG	2 cut(s)	20, 854
MspR9I	CCNGG	6 cut(s)	156, 461, 564, 2007, 2049, 2094
MunI	CAATTG	2 cut(s)	978, 2016
Mva1269I	GAATGC	2 cut(s)	542, 1905
MvaI	CCWGG	6 cut(s)	156, 461, 564, 2007, 2049, 2094
MvnI	CGCG	1 cut(s)	585
MwoI	GCNNNNNNNGC	5 cut(s)	11, 20, 42, 646, 2162
NcoI	CCATGG	2 cut(s)	715, 1482
NlaIV	GGNNCC	7 cut(s)	722, 991, 992, 1122, 1220, 1783, 1795
NmeAIII	GCCGAG	1 cut(s)	1685
NmuCI	GTSAC	3 cut(s)	332, 934, 1293
NspI	RCATGY	3 cut(s)	1324, 1670, 2156
OliI	CACNNNNGTG	1 cut(s)	930
PaeI	GCATGC	3 cut(s)	1324, 1670, 2156
PagI	TCATGA	1 cut(s)	2188
PceI	AGGCCT	1 cut(s)	1605
PciSI	GCTCTTC	1 cut(s)	685
PctI	GAATGC	2 cut(s)	542, 1905
PfeI	GAWTC	6 cut(s)	151, 230, 313, 755, 776, 1391
PflMI	CCANNNNNTGG	1 cut(s)	1940
PfoI	TCCNGGA	2 cut(s)	154, 2092
PkrI	GCNGC	6 cut(s)	197, 1099, 1164, 1303, 1458, 1672
PleI	GAGTC	1 cut(s)	1343
PpsI	GAGTC	1 cut(s)	1343
PpuMI	RGGWCCY	2 cut(s)	990, 1219
PsiI	TTATAA	1 cut(s)	1439
Psp5II	RGGWCCY	2 cut(s)	990, 1219
Psp6I	CCWGG	6 cut(s)	154, 459, 562, 2005, 2047, 2092
PspFI	CCCAGC	2 cut(s)	909, 2196
PspGI	CCWGG	6 cut(s)	154, 459, 562, 2005, 2047, 2092
PspN4I	GGNNCC	7 cut(s)	722, 991, 992, 1122, 1220, 1783, 1795
PspPI	GGNCC	6 cut(s)	34, 865, 990, 1219, 1561, 2045
PspPPI	RGGWCCY	2 cut(s)	990, 1219
PstI	CTGCAG	1 cut(s)	806
PsuI	RGATCY	4 cut(s)	798, 1120, 1333, 1591
PvuII	CAGCTG	1 cut(s)	1100
RsaI	GTAC	5 cut(s)	18, 608, 1289, 1543, 1783
RsaNI	GTAC	5 cut(s)	17, 607, 1288, 1542, 1782
RseI	CAYNNNNRTG	3 cut(s)	930, 1230, 1758
Rsr2I	CGGWCCG	1 cut(s)	1561
RsrII	CGGWCCG	1 cut(s)	1561
SapI	GCTCTTC	1 cut(s)	685
SaqAI	TTAA	3 cut(s)	111, 450, 1329
SatI	GCNGC	6 cut(s)	196, 1098, 1163, 1302, 1457, 1671
Sau96I	GGNCC	6 cut(s)	34, 865, 990, 1219, 1561, 2045
SchI	GAGTC	1 cut(s)	1343
ScrFI	CCNGG	6 cut(s)	156, 461, 564, 2007, 2049, 2094
SduI	GDGCHC	4 cut(s)	642, 1798, 1978, 2092
SfaNI	GCATC	6 cut(s)	194, 310, 424, 1234, 2053, 2152
SfcI	CTRYAG	2 cut(s)	354, 802
SinI	GGWCC	5 cut(s)	865, 990, 1219, 1561, 2045
SmiMI	CAYNNNNRTG	3 cut(s)	930, 1230, 1758
SmlI	CTYRAG	2 cut(s)	176, 635
SmoI	CTYRAG	2 cut(s)	176, 635
SphI	GCATGC	3 cut(s)	1324, 1670, 2156
SseBI	AGGCCT	1 cut(s)	1605
SsiI	CCGC	5 cut(s)	106, 1162, 1790, 1903, 2213
SspMI	CTAG	3 cut(s)	126, 213, 762
StuI	AGGCCT	1 cut(s)	1605
StyD4I	CCNGG	6 cut(s)	154, 459, 562, 2005, 2047, 2092
StyI	CCWWGG	3 cut(s)	715, 1084, 1482
TaaI	ACNGT	6 cut(s)	247, 268, 358, 412, 1345, 2125
TaqI	TCGA	4 cut(s)	149, 1059, 1352, 1854
TaqII	GACCGA	1 cut(s)	1549
TatI	WGTACW	2 cut(s)	606, 1287
TauI	GCSGC	1 cut(s)	1165
TfiI	GAWTC	6 cut(s)	151, 230, 313, 755, 776, 1391
Tru1I	TTAA	3 cut(s)	111, 450, 1329
Tru9I	TTAA	3 cut(s)	111, 450, 1329
TscAI	CASTG	5 cut(s)	141, 301, 943, 1188, 1648
TseFI	GTSAC	3 cut(s)	332, 934, 1293
TseI	GCWGC	5 cut(s)	195, 1097, 1301, 1456, 1670
Tsp45I	GTSAC	3 cut(s)	332, 934, 1293
TspDTI	ATGAA	7 cut(s)	491, 854, 983, 1587, 1596, 1742, 1849
TspGWI	ACGGA	3 cut(s)	979, 1553, 1931
TspRI	CASTG	5 cut(s)	141, 301, 943, 1188, 1648
Van91I	CCANNNNNTGG	1 cut(s)	1940
VneI	GTGCAC	1 cut(s)	1974
VpaK11BI	GGWCC	5 cut(s)	865, 990, 1219, 1561, 2045
XagI	CCTNNNNNAGG	1 cut(s)	998
XapI	RAATTY	5 cut(s)	454, 549, 568, 1495, 1749
XceI	RCATGY	3 cut(s)	1324, 1670, 2156
XmiI	GTMKAC	1 cut(s)	2202
XspI	CTAG	3 cut(s)	126, 213, 762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.