MD03G1119200.v1.1

Serine threonine-protein phosphatase 6 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008361
Physical Location & Seq
Forward (+)
10901575 .. 10911999
10425 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1119200.v1.1.491

Sequence Viewer

Length: 2562 bp
ATGTTTTGGAAGCTCACTTCTCTTTCTGCCTCATCGCCTGTGGAGACAGTGCTAGACAAGGAAAATTTTACATTAGAGGAGCTTCTGGATGAAGAAGAGATCATCCAAGAGTGCAAAGCCTTAAACACTCGTCTCATTAATTTTTTACGAGATAGAGACCAGGTGGAGCAATTATTGCGTTATATTGTTGAAGAAGCTCCTGAGGATGCTGAAAGCAAACGAGCCTTCAAGTTCCCCTTCATTGCTTGTGAGGTTTTTACATGTGAAATTGATGTTATCTTAAAGACTTTGGTGGATGAAGAGAAGCTAATGAACTTACTTTTCTCCTTCTTGGAACCAAATCGTCCTCATAGTGCCCCGCTGGCTGGGTATTTCAGCAAGGTTGTTGTATGCCTAATGATGCGGAAGACAGTCCCACTTATGAATTATGTTCAAGCCCATCAGGATGTTTTCCGTCAGCTAGTTGATTTGATAGGAATTACGTCCATCATGGAGGTTTTGGTTCGACTTGTTGGTGCAGATGACCATGTGTATCCCAATTTTGTTGATGTGATGCAATGGTTAGCTGAAAGTAATCTGCTTGAAATGATCGTAGATAAGTTGAGCCCAAATAATCCTCCTGAAGTTCATGCTAATGCAGCAGAAACACTTTCTGCTATAACTCGAAATGCTCCATCGGCCCTAGCCAACAAACTTTCCAGTCCAAGTTTTGTTACAAGAATATTTGGTCATGCATTGGAAGATTCACATTCAAAGTCTGGTCTTGTACACTCGCTTTCGGTCTGCATCTCTTTGTTGGATCCGAAAAGATCAGCAGTTTCTTCTCCCTTGTTCCATTCTTTTCGGAGCCAACATATGTACGAGTCTCCAATCCCTGTTAATCCAGATACTGTCAGTACAATGCTCCCAAAACTTGGCGACTTGCTTATGCTTTTGAATGTGTCATCGGATGAGAAAACATTGCCAACAACATACGGAGAGTTGAGGCCTCCACTTGGAAAGCATCGTTTAAAGATTGTGGAGTTCATTGCTGTTCTACTGAGATCAGGCAATGAAGAGGCAGAAAAGGAATTGGTTAGCTCGGGAACCATCCAACGGGTTATTGATCTTTTCTTTGAGTACCCGTACAATAGTTCCTTGCATCATCATGTAGATAGTATAATATCGTCATGCTTGGAAAGCAAGAGTGATGCCATTGTTGACCATCTTTTTCGAGAGTGTGATTTAATTGGAAAGTTCCTTCAAACAGATAAACATCCTATTCTATCTGGTGATGCTAATAAGCCAACAGTACCTGCTGCCGGAAAATCAGCACCACGGGCAGGAAACCTTGGGCACATAACACGAATTTCTAATAAGCTCATTCAATTGGGAAACAGCCAAAGCCGTGTTCAGGCATGCCTTCAGGAAAATAGCGAATGGAATGAGTGGCAAACAACAGTTTTGCAGGGGCGCAATGCAGTTGAAAATGTTTATCGATGGGCTTGCGGTCGCCCAACTGCTTTGCAAGACAGGACAAGGGATAGCGACGAGGATGACATGAATGACAGAAATTTTGATGTAGCAGCTCTAGCAAATAATCTAAGCCAGGCATTTAGATACAAAATTTATGAGAATGAGGACGCAGGAGAGGACCAGGGAGCTCATGATCGAGATGATGAGGATGTCTACTTTGACGATGAATCTGCTGAAGTGGTCATATCATCCTTGCGTCTTGGTGATGACCAAGGAAGCAGCAGTTTATTCACAAATTCCAACTGGTTTGCATTCCAAGATGATAGAATTGGTGATGAACCTGTGGGCACATCACTGTCGGAGATGGAAGAAGTAAACTTGAACGGGACTGCGAATGGCGGTAATAGCAGTAGTGATGATGAAGTTGTGGTAGGGGATGATGATGAGTTGGCCGGAAGTAAAGATTCCATCAATGGCACATCCAGTTCCAACACAAACCTTATGAATGGATTTAGCAACAGTGGAAACATGAACCCAGATGGCGAGAAGACAAGTGCATCCAATGAATTGGGGGGTTTCTTCAGATTTGAGACATCCGATAATGATGACATGTATGGAGACAGGCCTTTGCCTGAATGGGTGGGATGGAGTGAAGCTTCAGATTTGCAATTCGGCGGTGCAAGTGTAAATCCATTTGAGGATCATGATGACTCGGTTGTCAATCCTTCCACTGCCGAGGTAGTGGCATCTGATGTTGGTGCTAGTTTGCACTCAAGTGGAGAATCTACACTTTCAAATGGCTCGCCTCCATCCTCCACTGCTTCTACTGCAGGGTCAGTGGGCAGTGATGTGAGCCAGAGATCAGCTGCTGTGCCATCACTGTTTGAAGAGGATGTTGAATTTGTCGGTGTGGAGTTGGAAGGTACTGAGAAGGCTATGGAGCAGGCTCTTAAAGAGGGCATAGTAGGGGAGGCAGGGCCATTGAAGAAAAACATTATGCCGAAGGTGCTAGAAAAGGAGAACCCTGCCGATGGTGAGGCTGCAAATAAGGAGTTCAATGACGCCAACTACTGGAGGGTTGATCAGGAAGTGGCTGTTTTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAPS

Protein Analysis

854

Amino Acids

93.86

Weight (kDa)

4.5

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAPS PF04499 131 - 491 9.4e-67 SIT4 phosphatase-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2171
Acc36I ACCTGC 1 cut(s) 1303
AccB7I CCANNNNNTGG 2 cut(s) 914, 2526
AccI GTMKAC 1 cut(s) 1668
AciI CCGC 5 cut(s) 359, 403, 1488, 1854, 2130
AclWI GGATC 3 cut(s) 794, 807, 2163
AcoI YGGCCR 1 cut(s) 1905
AcsI RAATTY 6 cut(s) 64, 1347, 1552, 1605, 1750, 2354
AcuI CTGAAG 5 cut(s) 642, 1388, 1710, 2020, 2097
AcyI GRCGYC 1 cut(s) 2517
AfaI GTAC 7 cut(s) 768, 860, 898, 1121, 1127, 1293, 2380
AfiI CCNNNNNNNGG 9 cut(s) 365, 914, 995, 1095, 1301, 1322, 1393, 2486, 2526
AflIII ACRYGT 2 cut(s) 260, 2064
AjnI CCWGG 3 cut(s) 159, 1587, 1635
AjuI GAANNNNNNNTTGG 2 cut(s) 1374, 1406
AleI CACNNNNGTG 1 cut(s) 2229
Alw21I GWGCWC 1 cut(s) 1645
Alw26I GTCTC 6 cut(s) 38, 137, 150, 870, 2039, 2067
AlwI GGATC 3 cut(s) 794, 807, 2163
AlwNI CAGNNNCTG 3 cut(s) 890, 1295, 2324
Ama87I CYCGRG 1 cut(s) 1081
AoxI GGCC 5 cut(s) 678, 986, 1905, 2078, 2432
ApeKI GCWGC 6 cut(s) 638, 1298, 1565, 1734, 2321, 2495
ApoI RAATTY 6 cut(s) 64, 1347, 1552, 1605, 1750, 2354
ArsI GACNNNNNNTTYG 2 cut(s) 1538, 1570
AseI ATTAAT 1 cut(s) 138
AspLEI GCGC 1 cut(s) 1455
AspS9I GGNCC 3 cut(s) 679, 1633, 2432
AsuHPI GGTGA 4 cut(s) 1283, 1730, 1799, 2501
AvaI CYCGRG 1 cut(s) 1081
AvaII GGWCC 1 cut(s) 1633
AxyI CCTNAGG 1 cut(s) 201
BaeGI GKGCMC 3 cut(s) 358, 1338, 1805
BamHI GGATCC 1 cut(s) 799
BanII GRGCYC 2 cut(s) 608, 1645
BbsI GAAGAC 2 cut(s) 413, 2009
Bbv12I GWGCWC 1 cut(s) 1645
BbvI GCAGC 6 cut(s) 650, 1285, 1577, 1746, 2308, 2482
BceAI ACGGC 1 cut(s) 1371
BcgI CGANNNNNNTGC 4 cut(s) 1467, 1501, 1667, 1701
BciT130I CCWGG 3 cut(s) 161, 1589, 1637
BciVI GTATCC 1 cut(s) 543
BclI TGATCA 1 cut(s) 2536
BcoDI GTCTC 6 cut(s) 38, 137, 150, 870, 2039, 2067
BfaI CTAG 6 cut(s) 53, 461, 683, 1571, 2217, 2465
BfmI CTRYAG 1 cut(s) 2283
BfuAI ACCTGC 1 cut(s) 1303
BfuI GTATCC 1 cut(s) 543
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 6 cut(s) 639, 1299, 1566, 1735, 2322, 2496
BlsI GCNGC 6 cut(s) 640, 1300, 1567, 1736, 2323, 2497
Bme1390I CCNGG 3 cut(s) 161, 1589, 1637
Bme18I GGWCC 1 cut(s) 1633
BmeT110I CYCGRG 1 cut(s) 1081
BmgT120I GGNCC 3 cut(s) 679, 1633, 2432
BmiI GGNNCC 4 cut(s) 336, 801, 848, 1087
BmrFI CCNGG 3 cut(s) 161, 1589, 1637
BpiI GAAGAC 2 cut(s) 413, 2009
BpmI CTGGAG 1 cut(s) 2548
BpuEI CTTGAG 1 cut(s) 2212
Bsa29I ATCGAT 1 cut(s) 1477
BsaBI GATNNNNATC 1 cut(s) 1254
BsaHI GRCGYC 1 cut(s) 2517
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 5 cut(s) 1316, 1330, 1636, 1726, 2190
Bsc4I CCNNNNNNNGG 9 cut(s) 365, 914, 995, 1095, 1301, 1322, 1393, 2486, 2526
Bse1I ACTGG 4 cut(s) 699, 1763, 1938, 2531
Bse21I CCTNAGG 1 cut(s) 201
Bse3DI GCAATG 6 cut(s) 240, 563, 959, 1026, 1057, 1462
Bse8I GATNNNNATC 1 cut(s) 1254
BseBI CCWGG 3 cut(s) 161, 1589, 1637
BseCI ATCGAT 1 cut(s) 1477
BseDI CCNNGG 5 cut(s) 1316, 1330, 1636, 1726, 2190
BseJI GATNNNNATC 1 cut(s) 1254
BseLI CCNNNNNNNGG 9 cut(s) 365, 914, 995, 1095, 1301, 1322, 1393, 2486, 2526
BseMI GCAATG 6 cut(s) 240, 563, 959, 1026, 1057, 1462
BseMII CTCAG 3 cut(s) 192, 1031, 2373
BseNI ACTGG 4 cut(s) 699, 1763, 1938, 2531
BseRI GAGGAG 1 cut(s) 92
BseSI GKGCMC 3 cut(s) 358, 1338, 1805
BseXI GCAGC 6 cut(s) 650, 1285, 1577, 1746, 2308, 2482
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 537
Bsh1285I CGRYCG 1 cut(s) 1492
BshFI GGCC 5 cut(s) 680, 988, 1907, 2080, 2434
BshVI ATCGAT 1 cut(s) 1477
BsiEI CGRYCG 1 cut(s) 1492
BsiHKAI GWGCWC 1 cut(s) 1645
BsiHKCI CYCGRG 1 cut(s) 1081
BsiSI CCGG 2 cut(s) 1302, 1908
BslFI GGGAC 2 cut(s) 398, 1855
BslI CCNNNNNNNGG 9 cut(s) 365, 914, 995, 1095, 1301, 1322, 1393, 2486, 2526
BsmAI GTCTC 6 cut(s) 38, 137, 150, 870, 2039, 2067
BsmBI CGTCTC 1 cut(s) 137
BsmFI GGGAC 2 cut(s) 398, 1855
BsmI GAATGC 1 cut(s) 1766
BsnI GGCC 5 cut(s) 680, 988, 1907, 2080, 2434
Bso31I GGTCTC 1 cut(s) 150
BsoBI CYCGRG 1 cut(s) 1081
Bsp1286I GDGCHC 5 cut(s) 358, 608, 1338, 1645, 1805
Bsp1407I TGTACA 1 cut(s) 766
BspACI CCGC 5 cut(s) 359, 403, 1488, 1854, 2130
BspANI GGCC 5 cut(s) 680, 988, 1907, 2080, 2434
BspCNI CTCAG 3 cut(s) 193, 1032, 2374
BspDI ATCGAT 1 cut(s) 1477
BspHI TCATGA 2 cut(s) 1645, 2158
BspLI GGNNCC 4 cut(s) 336, 801, 848, 1087
BspMAI CTGCAG 1 cut(s) 2287
BspMI ACCTGC 1 cut(s) 1303
BspPI GGATC 3 cut(s) 794, 807, 2163
BspTNI GGTCTC 1 cut(s) 150
BsrDI GCAATG 6 cut(s) 240, 563, 959, 1026, 1057, 1462
BsrGI TGTACA 1 cut(s) 766
BsrI ACTGG 4 cut(s) 699, 1763, 1938, 2531
BssECI CCNNGG 5 cut(s) 1316, 1330, 1636, 1726, 2190
BssNI GRCGYC 1 cut(s) 2517
BssT1I CCWWGG 2 cut(s) 1330, 1726
Bst2UI CCWGG 3 cut(s) 161, 1589, 1637
Bst4CI ACNGT 8 cut(s) 49, 412, 892, 1291, 1441, 1812, 1976, 2337
Bst6I CTCTTC 4 cut(s) 90, 294, 1050, 2337
BstACI GRCGYC 1 cut(s) 2517
BstAPI GCANNNNNTGC 1 cut(s) 175
BstAUI TGTACA 1 cut(s) 766
BstC8I GCNNGC 5 cut(s) 363, 1399, 1486, 2258, 2400
BstDEI CTNAG 4 cut(s) 201, 1040, 1583, 2382
BstDSI CCRYGG 1 cut(s) 1316
BstHHI GCGC 1 cut(s) 1455
BstMAI GTCTC 6 cut(s) 38, 137, 150, 870, 2039, 2067
BstMCI CGRYCG 1 cut(s) 1492
BstNI CCWGG 3 cut(s) 161, 1589, 1637
BstNSI RCATGY 3 cut(s) 264, 1401, 2068
BstSCI CCNGG 3 cut(s) 159, 1587, 1635
BstSFI CTRYAG 1 cut(s) 2283
BstSLI GKGCMC 3 cut(s) 358, 1338, 1805
BstV1I GCAGC 6 cut(s) 650, 1285, 1577, 1746, 2308, 2482
BstV2I GAAGAC 2 cut(s) 413, 2009
BstX2I RGATCY 1 cut(s) 799
BstXI CCANNNNNNTGG 1 cut(s) 2023
BstYI RGATCY 1 cut(s) 799
Bsu15I ATCGAT 1 cut(s) 1477
Bsu36I CCTNAGG 1 cut(s) 201
BsuI GTATCC 1 cut(s) 543
BsuRI GGCC 5 cut(s) 680, 988, 1907, 2080, 2434
BsuTUI ATCGAT 1 cut(s) 1477
BtgI CCRYGG 1 cut(s) 1316
BtgZI GCGATG 1 cut(s) 18
BtsI GCAGTG 3 cut(s) 2184, 2271, 2305
BtsIMutI CAGTG 8 cut(s) 54, 1808, 1981, 2184, 2271, 2298, 2305, 2333
BveI ACCTGC 1 cut(s) 1303
Cac8I GCNNGC 5 cut(s) 363, 1399, 1486, 2258, 2400
CaiI CAGNNNCTG 3 cut(s) 890, 1295, 2324
CciI TCATGA 2 cut(s) 1645, 2158
CfoI GCGC 1 cut(s) 1455
Cfr13I GGNCC 3 cut(s) 679, 1633, 2432
ClaI ATCGAT 1 cut(s) 1477
CseI GACGC 3 cut(s) 1631, 1700, 2525
CsiI ACCWGGT 1 cut(s) 159
Csp6I GTAC 7 cut(s) 767, 859, 897, 1120, 1126, 1292, 2379
CviQI GTAC 7 cut(s) 767, 859, 897, 1120, 1126, 1292, 2379
DdeI CTNAG 4 cut(s) 201, 1040, 1583, 2382
DraI TTTAAA 1 cut(s) 1011
DrdI GACNNNNNNGTC 1 cut(s) 2171
DseDI GACNNNNNNGTC 1 cut(s) 2171
EaeI YGGCCR 1 cut(s) 1905
Eam1104I CTCTTC 4 cut(s) 90, 294, 1050, 2337
EarI CTCTTC 4 cut(s) 90, 294, 1050, 2337
Ecl136II GAGCTC 1 cut(s) 1643
Eco130I CCWWGG 2 cut(s) 1330, 1726
Eco147I AGGCCT 2 cut(s) 988, 2080
Eco24I GRGCYC 2 cut(s) 608, 1645
Eco31I GGTCTC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 1633
Eco53kI GAGCTC 1 cut(s) 1643
Eco57I CTGAAG 5 cut(s) 642, 1388, 1710, 2020, 2097
Eco81I CCTNAGG 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 1081
EcoICRI GAGCTC 1 cut(s) 1643
EcoRII CCWGG 3 cut(s) 159, 1587, 1635
EcoT14I CCWWGG 2 cut(s) 1330, 1726
EcoT22I ATGCAT 1 cut(s) 736
EcoT38I GRGCYC 2 cut(s) 608, 1645
ErhI CCWWGG 2 cut(s) 1330, 1726
Esp3I CGTCTC 1 cut(s) 137
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FaqI GGGAC 2 cut(s) 398, 1855
FauI CCCGC 1 cut(s) 366
FauNDI CATATG 1 cut(s) 855
FbaI TGATCA 1 cut(s) 2536
FblI GTMKAC 1 cut(s) 1668
Fnu4HI GCNGC 6 cut(s) 639, 1299, 1566, 1735, 2322, 2496
FriOI GRGCYC 2 cut(s) 608, 1645
Fsp4HI GCNGC 6 cut(s) 639, 1299, 1566, 1735, 2322, 2496
FspBI CTAG 6 cut(s) 53, 461, 683, 1571, 2217, 2465
GlaI GCGC 1 cut(s) 1454
GluI GCNGC 6 cut(s) 639, 1299, 1566, 1735, 2322, 2496
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 2548
HaeIII GGCC 5 cut(s) 680, 988, 1907, 2080, 2434
HapII CCGG 2 cut(s) 1302, 1908
HgaI GACGC 3 cut(s) 1631, 1700, 2525
HhaI GCGC 1 cut(s) 1455
Hin1I GRCGYC 1 cut(s) 2517
Hin6I GCGC 1 cut(s) 1453
HinP1I GCGC 1 cut(s) 1453
HincII GTYRAC 1 cut(s) 1201
HindII GTYRAC 1 cut(s) 1201
HindIII AAGCTT 1 cut(s) 2109
HinfI GANTC 6 cut(s) 743, 863, 1682, 1919, 2165, 2237
HpaII CCGG 2 cut(s) 1302, 1908
HphI GGTGA 4 cut(s) 1283, 1730, 1799, 2501
Hpy166II GTNNAC 4 cut(s) 769, 1201, 1669, 1831
Hpy188I TCNGA 8 cut(s) 804, 846, 949, 1816, 2039, 2053, 2116, 2206
Hpy8I GTNNAC 4 cut(s) 769, 1201, 1669, 1831
Hpy99I CGWCG 1 cut(s) 1532
HpyAV CCTTC 9 cut(s) 235, 247, 337, 1250, 1412, 2190, 2369, 2380, 2452
HpyCH4III ACNGT 8 cut(s) 49, 412, 892, 1291, 1441, 1812, 1976, 2337
HpyCH4IV ACGT 1 cut(s) 482
HpyF3I CTNAG 4 cut(s) 201, 1040, 1583, 2382
HpySE526I ACGT 1 cut(s) 482
Hsp92I GRCGYC 1 cut(s) 2517
HspAI GCGC 1 cut(s) 1453
Ksp22I TGATCA 1 cut(s) 2536
LmnI GCTCC 8 cut(s) 79, 166, 202, 676, 846, 909, 1640, 2395
Lsp1109I GCAGC 6 cut(s) 650, 1285, 1577, 1746, 2308, 2482
MabI ACCWGGT 1 cut(s) 159
MaeI CTAG 6 cut(s) 53, 461, 683, 1571, 2217, 2465
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 1 cut(s) 712
MfeI CAATTG 1 cut(s) 1367
MflI RGATCY 1 cut(s) 799
MhlI GDGCHC 5 cut(s) 358, 608, 1338, 1645, 1805
MlyI GAGTC 2 cut(s) 872, 2159
MmeI TCCRAC 6 cut(s) 777, 1117, 1779, 1794, 1968, 2352
Mph1103I ATGCAT 1 cut(s) 736
MseI TTAA 7 cut(s) 122, 138, 281, 879, 1010, 1226, 2406
MslI CAYNNNNRTG 4 cut(s) 444, 633, 1146, 2229
MspA1I CMGCKG 2 cut(s) 361, 2321
MspI CCGG 2 cut(s) 1302, 1908
MspR9I CCNGG 3 cut(s) 161, 1589, 1637
MunI CAATTG 1 cut(s) 1367
Mva1269I GAATGC 1 cut(s) 1766
MvaI CCWGG 3 cut(s) 161, 1589, 1637
NdeI CATATG 1 cut(s) 855
NlaIV GGNNCC 4 cut(s) 336, 801, 848, 1087
NmeAIII GCCGAG 1 cut(s) 2215
NsiI ATGCAT 1 cut(s) 736
NspI RCATGY 3 cut(s) 264, 1401, 2068
OliI CACNNNNGTG 1 cut(s) 2229
PaeI GCATGC 1 cut(s) 1401
PagI TCATGA 2 cut(s) 1645, 2158
PceI AGGCCT 2 cut(s) 988, 2080
PciI ACATGT 2 cut(s) 260, 2064
PctI GAATGC 1 cut(s) 1766
PfeI GAWTC 4 cut(s) 743, 1682, 1919, 2237
PflMI CCANNNNNTGG 2 cut(s) 914, 2526
PkrI GCNGC 6 cut(s) 640, 1300, 1567, 1736, 2323, 2497
PleI GAGTC 2 cut(s) 871, 2159
PpsI GAGTC 2 cut(s) 871, 2159
PscI ACATGT 2 cut(s) 260, 2064
PshBI ATTAAT 1 cut(s) 138
Psp124BI GAGCTC 1 cut(s) 1645
Psp6I CCWGG 3 cut(s) 159, 1587, 1635
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 3 cut(s) 159, 1587, 1635
PspN4I GGNNCC 4 cut(s) 336, 801, 848, 1087
PspPI GGNCC 3 cut(s) 679, 1633, 2432
PstI CTGCAG 1 cut(s) 2287
PstNI CAGNNNCTG 3 cut(s) 890, 1295, 2324
PsuI RGATCY 1 cut(s) 799
PvuII CAGCTG 1 cut(s) 2321
RsaI GTAC 7 cut(s) 768, 860, 898, 1121, 1127, 1293, 2380
RsaNI GTAC 7 cut(s) 767, 859, 897, 1120, 1126, 1292, 2379
RseI CAYNNNNRTG 4 cut(s) 444, 633, 1146, 2229
SacI GAGCTC 1 cut(s) 1645
SaqAI TTAA 7 cut(s) 122, 138, 281, 879, 1010, 1226, 2406
SatI GCNGC 6 cut(s) 639, 1299, 1566, 1735, 2322, 2496
Sau96I GGNCC 3 cut(s) 679, 1633, 2432
SchI GAGTC 2 cut(s) 872, 2159
ScrFI CCNGG 3 cut(s) 161, 1589, 1637
SduI GDGCHC 5 cut(s) 358, 608, 1338, 1645, 1805
SexAI ACCWGGT 1 cut(s) 159
SfcI CTRYAG 1 cut(s) 2283
SinI GGWCC 1 cut(s) 1633
SmiMI CAYNNNNRTG 4 cut(s) 444, 633, 1146, 2229
SmlI CTYRAG 1 cut(s) 2227
SmoI CTYRAG 1 cut(s) 2227
SphI GCATGC 1 cut(s) 1401
SseBI AGGCCT 2 cut(s) 988, 2080
SsiI CCGC 5 cut(s) 359, 403, 1488, 1854, 2130
SspI AATATT 1 cut(s) 723
SspMI CTAG 6 cut(s) 53, 461, 683, 1571, 2217, 2465
SstI GAGCTC 1 cut(s) 1645
StuI AGGCCT 2 cut(s) 988, 2080
StyD4I CCNGG 3 cut(s) 159, 1587, 1635
StyI CCWWGG 2 cut(s) 1330, 1726
TaaI ACNGT 8 cut(s) 49, 412, 892, 1291, 1441, 1812, 1976, 2337
TaiI ACGT 1 cut(s) 485
TaqI TCGA 5 cut(s) 505, 664, 1213, 1477, 1651
TaqII GACCGA 1 cut(s) 769
TatI WGTACW 2 cut(s) 766, 896
TfiI GAWTC 4 cut(s) 743, 1682, 1919, 2237
Tru1I TTAA 7 cut(s) 122, 138, 281, 879, 1010, 1226, 2406
Tru9I TTAA 7 cut(s) 122, 138, 281, 879, 1010, 1226, 2406
TscAI CASTG 8 cut(s) 54, 1815, 1981, 2191, 2278, 2298, 2305, 2340
TseI GCWGC 6 cut(s) 638, 1298, 1565, 1734, 2321, 2495
TspGWI ACGGA 2 cut(s) 443, 990
TspRI CASTG 8 cut(s) 54, 1815, 1981, 2191, 2278, 2298, 2305, 2340
Van91I CCANNNNNTGG 2 cut(s) 914, 2526
VpaK11BI GGWCC 1 cut(s) 1633
VspI ATTAAT 1 cut(s) 138
XapI RAATTY 6 cut(s) 64, 1347, 1552, 1605, 1750, 2354
XceI RCATGY 3 cut(s) 264, 1401, 2068
XmiI GTMKAC 1 cut(s) 1668
XspI CTAG 6 cut(s) 53, 461, 683, 1571, 2217, 2465
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.